Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 5,612,860 C→T 5.8% A597T (GCC→ACC)  msbA ← Lipid A export ATP‑binding/permease proteinMsbA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1435,612,8600CT 5.8% 235.7 / 3.5 69A597T (GCC→ACC) msbALipid A export ATP‑binding/permease proteinMsbA
Reads supporting (aligned to +/- strand):  ref base C (34/31);  new base T (2/2);  total (36/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.56e-01

AATAATGAACAGGGGCAGGATATTAGCACAGGGTAGTCAATGGCTTTATGGCCAGATCATCCCTGGAAAATTCCCGTGACAATCGAGGCCATGACGGCCCCGATCAAGGAGGGTTCAGGTGATATCGGCCTTGGCCGGCTCATCCAGCCCCATGGCATGCAAACGGGCATAATGGCCATTAGCCGCAAGCAGCTCGGTGTGAGTACCGCGCTCGACCAGGCGGCCCTGGTCCATGACAAGGATCTGGTCAGCCTTCTCGATGGTCGA  >  ACB143/5612726‑5612992
                                                                                                                                      |                                                                                                                                    
aataatGAACCGGGGCAGCATATTAGCACAGGGTAGTCAATGGCTTTATGGCCAGATCATCCCTGGAAAATTCCCGTGACAATCGAGGCCATGACGGCCCCGATCAAGGAGGGTTCAGGTGATATCGGCCTTGGcc                                                                                                                                     <  4:332119/136‑1 (MQ=255)
   aatGAACAGGGGCAGGATATTAGCACAGGGTAGTCAATGGCTTTATGGCCAGATCATCCCTGGAAAATTCCCGTGACAATCGAGGCCATGACGGCCCCGATCAAGGAGGGTTCAGGTGATATCGGCCTTGGCCGGc                                                                                                                                  >  8:210804/1‑136 (MQ=255)
    atGAACAGGGGCAGGATATTAGCACAGGGTAGTCAATGGCTTTATGGCCAGATCATCCCTGGAAAATTCCCGTGACAATCGAGGCCATGACGGCCCCGATCAAGGAGGGTTCAGGTGATATCGGCCTTGGCCTGCt                                                                                                                                 >  4:85644/1‑136 (MQ=255)
     ttaaCAGGGGCAGGATATTAGCACAGGGTAGTCAATGGCTTTATGGCCAGATCATCCCTGGAAAATTCCCGTGACAATCGAGTCCATGACGGCCCCGATCAAGGAGGGTTCAGGTGATATCGGCCTTGGCCGGCTc                                                                                                                                <  1:153137/134‑1 (MQ=255)
     tGAACAGGGGCAGTATATTAGCACAGGGTAGTCAATGGCGTTATGGCCAGATCATCCCTGGAAAATTCCCGTGACAATCGAGGCCATGACGGCCCCGATCAAGGAGGGTTCAGGTGATATCGGCCTTGGCCGGCTc                                                                                                                                <  1:160177/136‑1 (MQ=255)
     tGAACAGGGGCAGGATATTCGCACAGGGTACTCAATGGCGTTATGGCCAGATCATCCCTGGAAAATTCCCGTGACAATCGAGGCCATGACGGCCCCGATCAAGGAGGGTTCAGGTGATATCGGCCTTGGCCGGCTc                                                                                                                                <  3:30459/136‑1 (MQ=255)
     tGAACAGGGGCAGGATATTAGCACAGGGTAGTCAATGGCTTTATGGCCAGATCATCCCTGGAAAATTCCCGTGACAATCGAGGCCATGACGGCCCCGATCAAGGAGGGTTCAGGTGATATCGGCCTTGGCCGGCTc                                                                                                                                <  8:102990/136‑1 (MQ=255)
      gAACTGGGGCAGGATATTTGCACAGGGCAGTCAATGGCTTTCTGGCCAGATCATCCCTGGAAAATTCCGGTGACAATCGAGGCCATGACGGCCCCGATCAAGGAGGGTTCAGGTGATATCGGCCTTGGCCGGCTc                                                                                                                                <  4:352974/135‑1 (MQ=255)
                aGGATATTAGCACAGGGTAGTCAATGGCTTTATGGCCAGATCATCCCTGGAAAATTCCCGTGACAATCGAGGCCATGACGGCCCCGATCAAGGAGGGTTCAGGTGATGTCGGCCTTGGCCGGCTCATCCAGCCCCa                                                                                                                     >  2:342413/1‑136 (MQ=255)
                  gATATTAGCACAGGGTAGTCAATGGCTTTATGGCCAGATCATCCCTGGAAAATTCCCGTGACAATCGAGGCCATGACGGCCCCGATCAAGGAGGGTTCAGGTGATATCGGCCTTGGCCGGCTCATCCAGCCCCATg                                                                                                                   >  6:257634/1‑136 (MQ=255)
                         gCACAGGGTAGTCAATGGCTTTATGGCCAGATCATCCCTGGAAAATTCCCGTGACAATCGAGGCCATGACGGCCCCGATCAAGGAGGGTTCAGGTGATATCGGCCTTGGCCGGCTCATCCCGCCCCATGGCATGc                                                                                                             >  6:366902/1‑135 (MQ=255)
                         gCACAGGGTAGTCAATGGCTTTATGGCCAGATCATCCCTGGAAAATTCCCGTGACAATCGAGGCCATGACGGCCCCGATCAAGGAGGGTTCAGGTGATATCGGCCTTGGCCGGCTCATCCAGCCCCATGGCATGc                                                                                                             <  8:51863/135‑1 (MQ=255)
                         gCACAGGGTAGTCAATGGCTTTATGGCCAGATCATCCCTGGAAAATTCCCGTGACAATCGAGGCCATGACGGCCCCGATCAAGGAGGGTTCAGGTGATATCGGCCTTGGCCGGCTCATCCAGCCCCATGGCATGc                                                                                                             >  1:104523/1‑135 (MQ=255)
                         gCACAGGGTAGTCAATGGCTTTATGGCCAGATCATCCCTGGAAAATTCCCGTGACAATCGAGGCCATGACGGCCCCGATCAAGGAGGGTTCAGGGGATATCGGCCTTGGCCGGCTCATCCAGCCCCATGGCATGc                                                                                                             <  8:57305/135‑1 (MQ=255)
                          cacaGGGTAGTCAATGGCTTTATGGCCAGATCATCCCTGGAAAATTCCCGTGACAATCGAGGCCATGACGGCCCCGATCAAGGAGGGTTCAGGTGATATCGGCCTTGGCCGGCTCATCCAGCCCCATGGCATGCa                                                                                                            >  6:274380/1‑135 (MQ=255)
                          cacaGGGTAGTCAATGGCTTTATGGCCAGATCATCCCTGGAAAATTCCCGTGACAATCGAGGCCATGACGGCCCCGATCAAGGAGGGTTCAGGTGATATCGGCCTTGGCCGGCTCATCCAGCCCCATGGCATGCa                                                                                                            >  7:158631/1‑135 (MQ=255)
                           acaGGGTAGTCAATGGCTTTATGGCCAGATCATCCCTGGAAAATTCCCGTGACAATCGAGGCCATGACGGCCCCGATCAAGGAGGGTTCAGGTGATATCGGCCTTGGCCGGCTCATCCAGCCCCATGGCATGCaaa                                                                                                          <  4:138580/136‑1 (MQ=255)
                               ggTAGTCAATGGCTTTATGGCCAGATCATCCCTGGAAAATTCCCGTGACAATCGAGGCCATGACGGCCCCGATCAAGGAGGGTTCAGGTGATATCGGCCTTGGCCGGCTCATCCAGCCCCATGGCATGCAAACggg                                                                                                      >  4:44319/1‑136 (MQ=255)
                               ggTAGTCAATGGCTTTATGGCCAGATCATCCCTGGAAAATTCCCGTGACAATCGAGGCCATGACGGCCCCGATCAAGGAGGGTTCAGGTGATATCGGCCTTGGCCGGCTCATCCAGCCCCATGGCATGCAAACggg                                                                                                      >  2:133383/1‑136 (MQ=255)
                                 tAGTCAATGGCTTTATGGCCAGATCATCCCTGGAAAATTCCCGTGACAATCGAGGCCATGACGGCCCCGATCAAGGAGGGTTCAGGTGATATCGGCCTTGGCCGGCTCATCCAGCCCCATGGCATGCAAACGGGCa                                                                                                    >  5:335436/1‑136 (MQ=255)
                                  aGTCAATGGCTTTATGGCCAGATCATCCCTGGAAAATTCCCGTGACAATCGAGGCCATGACGGCCCCGATCAAGGAGGGTTCAGGTGATATCGGCCTTGGCCGGCTCATCCAGCCCCATGGCATGCAAACGGGCa                                                                                                    >  2:378077/1‑135 (MQ=255)
                                  aGTCAATGGCTTTATGGCCAGATCATCCCTGGAAAATTCCCGTGACAATCGAGGCCATGACGGCCCCGATCAAGGAGGGTTCAGGTGATATCGGCCTTGGCCGGCTCATCCAGCCCCATGGCATGCAAACGGGCa                                                                                                    >  1:98980/1‑135 (MQ=255)
                                  aGTCAATGGCTTTATGGCCAGATCATCCCTGGAAAATTCCCGTGACAATCGAGGCCATGACGGCCCCGATCAAGGAGGGTTCAGGTGATATCGGCCTTGGCCGGCTCATCCAGCCCCATGGCATGCAAACGGGCa                                                                                                    >  4:227768/1‑135 (MQ=255)
                                  aGTCAATGGCTTTATGGCCAGATCATCCCTGGAAAATTCCCGTGACAATCGAGGCCATGACGGCCCCGATCAAGGAGGGTTCAGGTGATATCGGCCTTGGCCGGCTCATCCAGCCCCATGGCATGCAAACGGGCa                                                                                                    >  8:331981/1‑135 (MQ=255)
                                  aGTCAATGGCTTTATGGCCAGATCATCCCTGGAAAATTCCCGTGACAATCGAGGCCATGACGGCCCCGATCAAGGAGGGTTCAGGTGATATCGGCCTTGGCCGGCTCATCCAGCCCCATGGCATGCAAACGGGCa                                                                                                    >  3:46276/1‑135 (MQ=255)
                                  aGTCAATGGCTTTATGGCCAGATCATCCCTGGAAAATTCCCGTGACAATCGAGGCCATGACGGCCCCGATCAAGGAGGGTTCAGGTGATATCGGCCTTGGCCGGCTCATCCAGCCCCATGGCATGCAAACGGGCa                                                                                                    >  2:229046/1‑135 (MQ=255)
                                        tGGCTTTATGGCCAGATCATCCCTGGAAAATTCCCGTGACAATCGAGGCCATGACGGCCCCGATCAAGGAGGGTTCAGGTGATATCGGCCTTGGCCGGCTCATCCAGCCCCATGGCATGCAAACGGGCATAATGGc                                                                                             <  5:257634/136‑1 (MQ=255)
                                            tttATGGCCAGATCATCCCTGGAAAATTCCCGTGACAATCGAGGCCATGACGGCCCCGATCAAGGAGGGTTCAGGTGATATCGGCCTTGGCCGGCTCATCCAGCCCCATGGCATGCAAACGGGCATAATGGCCAt                                                                                          >  2:134741/1‑135 (MQ=255)
                                                 ggCCAGATCATCCCTGGAAAATTCCCGTGACAATCGAGGCCATGACGGCGCCGATCAAGGAGGGTTCAGGTGATATCGGCCTTGGCCGGCTCATCCAGCCCCATGGCATGCAAACGGGCATAATGGCCATTAGCCg                                                                                    >  7:145524/1‑136 (MQ=255)
                                                 ggCCAGATCATCCCTGGAAAATTCCCGTGACAATCGAGGCCATGACGGCCCCGATCAAGGAGGGTTCAGGTGATATCGGCCTTGGCCGGCTCATCCAGCCCCATGGCATGCAAACGGGCATAATGGCCATTAGCCg                                                                                    >  3:82268/1‑136 (MQ=255)
                                                 ggCCAGATCATCCCTGGAAAATTCCCGTGACAATCGAGGCCATGACGGCCCCGATCAAGGAGGGTTCAGGTGATATCGGCCTTGGCCGGCTCATCCAGCCCCATGGCATGCAAACGGGCATAATGGCCATTAGCCg                                                                                    >  7:88507/1‑136 (MQ=255)
                                                  gCCAGATCATCCCTGGAAAATTCCCGTGACAATCGAGGCCATGACGGCCCCGATCAAGGAGGGTTCAGGTGATATCGGCCTTGGCCGGCTCATCCAGCCCCATGGCATGCAAACGGGCATAATGGCCATTAGCCGc                                                                                   <  4:46683/136‑1 (MQ=255)
                                                       atcatcCCTGGAAAATTCCCGTGACAATCGAGGCCAGGACGGCCCCGATCAAGGAGGGTTCAGGTGATATCGGCCTTGGCCGGCTCATCCAGCCCCATGGCATGCAAACGGGCATAATGGCCATTAGCCGCAagca                                                                              <  2:151903/136‑1 (MQ=255)
                                                        tcatcCCTGGAAAATTCCCGTGACAATCGAGGCCATGACGGCCCCGATCAAGGAGGGTTCAGGTGATATCGGCCTTGGCCGGCTCATCCAGCCCCATGGCATGCAAACGGGCATAATGGCCATTAGCCGCAagcag                                                                             <  3:46374/136‑1 (MQ=255)
                                                        tcatcCCTGGAAAATTCCCGTGACAATCGAGGCCATGACGGCCCCGATCAAGGAGGGTTCAGGTGATATCGGCCTTGGCCGGCTCATCCAGCCCCATGGCATGCAAACGGGCATAATGGCCATTAGCCGCAagcag                                                                             <  1:126009/136‑1 (MQ=255)
                                                        tcatcCCTGGAAAATTCCCGTGACAATCGAGGCCATGACGGCCCCGATCAAGGAGGGTTCAGGTGATATCGGCCTTGGCCGGCTCATCCAGCCCCATGGCATGCAAACGGGCATAATGGCCATTAGCCGCAagcag                                                                             >  3:342024/1‑136 (MQ=255)
                                                           tcCCTGGAAAATTCCCGTGACAATCGAGGCCATGACGGCCCCGATCAAGGAGGGTTCAGGTGATATCGGCCTTGGCCGGCTCATCCAGCCCCATGGCATGCAAACGGGCATAATGGCCATTAGCCGCAAGCAGCTc                                                                          <  8:261106/136‑1 (MQ=255)
                                                           tcCCTGGAAAATTCCCGTGACAATCGAGGCCATGACGGCCCCGATCAAGGAGGGTTCAGGTGATATCGGCCTTGGCCGGCTCATCCAGCCCCATGGCATGCAAACGGGCATAATGGCCATTAGCCGCAAGCAGCTc                                                                          <  6:72626/136‑1 (MQ=255)
                                                             ccTGGAAAATTCCCGTTACAATCGAGGCCATGACGGCCCCGATCAAGGAGGGTTCAGGTGATATCGGCCTTGGCCGGCTCATCCAGCCCCATGGCATGCAAACGGGCATAATGGCCATTAGCCGCAAGCAGCTCgg                                                                        <  2:216278/136‑1 (MQ=255)
                                                                                 aTCGAGGCCATGACGGCCCCGATCAAGGAGGGTTCAGGTGATATCGGCCTTGGCCGGATCATCCAGCCCCATGGCATGCAAACGGGCATAATGGCCATTAGCCGCAAGCAGCTCGGTGTGAGTACCGCGCTCGAcc                                                    <  7:278013/136‑1 (MQ=255)
                                                                                       gCCATGACGGCCCCGATCAAGGAGGGTTCAGGTGATATCGGCCTTGGCCGGCTCATCCAGCCCCATGGCATGCAAACGGGCATAATGGCCATTAGCCGCAAGCAGCTCGGTGTGAGTACCGCGCTCGACCAggcgg                                              <  3:117252/136‑1 (MQ=255)
                                                                                       gCCATGACGGCCCCGATCAAGGAGGGTTCAGGTGATATCGGCCTTGGCCGGCTCATCCAGCCCCATGGCATGCAAACGGGCATAATGGCCATTAGCCGCAAGCAGCTCGGTGTGAGTACCGCGCGCGACCAggcgg                                              <  7:190770/136‑1 (MQ=255)
                                                                                         aaTGACGGCCCCGATCAAGGAGGGTTCAGGTGCTATCGGCCTTGGCCGGCTCATCCAGCCCCATGGCATGCAAACGGGCATAATGGCCATTAGCCGCAAGCAGCTCGGTGTGAGTCCCGCGCTCGACCAGGCGGcc                                            <  5:306461/135‑1 (MQ=255)
                                                                                             aCGGCCCCGATCAAGGAGGGTTCAGGTGATATCGGCCTTGGCCGGCTCATCCAGCCCCATGGCATGCAAACGGGCATAATGGCCATTAGCCGCAAGCAGCTCGGTGTGAGTACCGCGCTCGACCAGGCGGCCCTg                                         <  1:68952/135‑1 (MQ=255)
                                                                                               ggCCCCGATCAAGGAGGGTTCAGGTGATATCGGCCTTGGCCGGCTCATCCAGCCCCATGGCATGCAAACGGGCATAATGGCCATTAGCCGCAAGCAGCTCGGTGTGAGTACCGCGCTCGACCAGGCGGCCCTGGTc                                      >  4:71826/1‑136 (MQ=255)
                                                                                                   ccGATCAAGGAGGGTTCAGGTGATATCGGCCTTGGCCGGCTCATCCAGCCCCATGGCATGCAAACGGGCATAATGGCCATTAGCCGCAAGCAGCTCGGTGTGAGTACCGCGCTCGACCAGGCGGCCCTGGTCCATg                                  <  1:133383/136‑1 (MQ=255)
                                                                                                   ccGATCAAGGAGGGTTCAGGTGATATCGGCCTTGGCCGGCTCATCCAGCCCCATGGCATGCAAACGGGCATAATGGCCATTAGCCGCAAGCAGCTCGGTGTGAGTACCGCGCTCGACCAGGCGGCCCTGGTCCATg                                  <  1:378077/136‑1 (MQ=255)
                                                                                                   ccGATCAAGGAGGGTTCAGGTGATATCGGCCTTGGCCGGCTCATCCAGCCCCATGGCATGCAAACGGGCATAATGGCCATTAGCCGCAAGCAGCTCGGTGTGAGTACCGCGCTCGACCAGGCGGCCCTGGTCCATg                                  <  1:125575/136‑1 (MQ=255)
                                                                                                   ccGATCAAGGAGGGTTCAGGTGATATCGGCCTTGGCCGGCTCATCCAGCCCCATGGCATGCAAACGGGCATAATGGCCATTAGCCGCAAGCAGCTCGGTGTGAGTACCGCGCTCGACCAGGCGGCCCTGGTCCATg                                  <  3:197031/136‑1 (MQ=255)
                                                                                                         aaGGAGGGTTCAGGTGATATCGGCCTTGGCCGGCTCATCCAGCCCCATGGCATGCAAACGGGCATAATGGCCATTAGCCGCAAGCAGCTCGGTGTGAGTACCGCGCTCGACCAGGCGGCCCTGGTCCATGACAAg                             >  1:278547/1‑135 (MQ=255)
                                                                                                         aaGGAGGGTTCAGGTGATATCGGCCTTGGCCGGCTCATCCAGCCCCATGGCATGCAAACGGGCATAATGGCCATTAGCCGCAAGCAGCTCGGTGTGAGTACCGCGCTCGACCAGGCGGCCCTGGTCCATGACAAg                             <  1:134741/135‑1 (MQ=255)
                                                                                                             aggGTTCAGGTGATATCGGCCTTGGCCGGCTCATCCAGCCCCATGGCATGCAAACGGGCATAATGGCCATTAGCCGCAAGCAGCTCGGTGTGAGTACCGCGCTCGACCAGGCGGCCCTGGTCCATGACAAGGATCt                        <  6:335436/136‑1 (MQ=255)
                                                                                                              gggTTCAGGTGATATCGGCCTTGGCCGGCTCATCCAGCCCCATGGCATGCAAACGGGc                                                                                                     <  5:339220/58‑1 (MQ=255)
                                                                                                              gggTTCAGGTGATATCGGCCTTGGCCGGCTCATCCAGCCCCATGGCATGCAAACGGGc                                                                                                     >  6:339220/1‑58 (MQ=255)
                                                                                                                  tCAGGTGATATCGGCCTTGGCCGGCTCATCCAGCCCCATGGCATGCAAACGGGCATAATGGCCATTAGCCGCAAGCAGCTCGGTGTGAGTACCGCGCTCGACCAGGCGGCCCTGGTCCATGACAAGGATCTGGt                     >  7:221398/1‑134 (MQ=255)
                                                                                                                   cAGGTGATATCGGCCTTGGCCGGCTCATCCAGCCCCATGGCATGCAAACGGGCATAATGGCCATTAGCCGCAAGCAGCTCGGTGTGAGTACCGCGCTCGACCAGGCGGCCCTGGTCCATGACAAGGATCTGGTCAg                  >  4:23352/1‑136 (MQ=255)
                                                                                                                    aGGTGATATCGGCCTTGGTCGGCTCATCCAGCCCCATGGCATGCAAACGGGCATAATGGCCATTAGCCGCAAGCAGCTCGGTGTGAGTACCGCGCTCGACCAggcgg                                              <  6:112/107‑1 (MQ=255)
                                                                                                                    aGGTGATATCGGCCTTGGTCGGCTCATCCAGCCCCATGGCATGCAAACGGGCATAATGGCCATTAGCCGCAAGCAGCTCGGTGTGAGTACCGCGCTCGACCAggcgg                                              <  6:116/107‑1 (MQ=255)
                                                                                                                    aGGTGATATCGGCCTTGGTCGGCTCATCCAGCCCCATGGCATGCAAACGGGCATAATGGCCATTAGCCGCAAGCAGCTCGGTGTGAGTACCGCGCTCGACCAggcgg                                              >  5:116/1‑107 (MQ=255)
                                                                                                                    aGGTGATATCGGCCTTGGTCGGCTCATCCAGCCCCATGGCATGCAAACGGGCATAATGGCCATTAGCCGCAAGCAGCTCGGTGTGAGTACCGCGCTCGACCAggcgg                                              >  5:112/1‑107 (MQ=255)
                                                                                                                      gTGATATCGGCCTTGGCCGGCTCATCCAGCCCCATGGCATGCAAACGGGCATAATGGCCATTAGCCGCAAGCAGCTCGGTGTGAGTACCGCGCTCGACCAGGCGGCCCTGGTCCATGACAAGGATCTGGTCAGCCt               <  1:177007/136‑1 (MQ=255)
                                                                                                                         atatCGGCCTTGGCCGGCTCATCCAGCCCCATGGCATGCAAACGGGCATAATGGCCATTAGCCGCAAGCAGCTCGGTGTGAGTACCGCGCTCGACCAGGCGGCCCTGGTCCATGACAAGGATCTGGTCAGCCTtct            >  5:109480/1‑136 (MQ=255)
                                                                                                                            tCGGCCTTGGCCGGCTCATCCAGCCCCATGGCATGCAAACGGGCATAATGGCCATTAGCCGCAAGCAGCTCGGTGTGAGTACCGCGCTCGACCCGGCGGCCCTGGTCCATGACAAGGATCTGGTCAGCCTTCTCGa         >  4:208099/1‑136 (MQ=255)
                                                                                                                            tCGGCCTTGGCCGGCTCATCCAGCCCCATGGCATGCAAACGGGCATAATGGCCATTAGCCGCAAGCAGCTCGGTGTGAGTACCGCGCTCGACCAGGCGGCCCTGGTCCATGACAAGGATCTGGTCAGCCTTCTCg          <  4:397775/135‑1 (MQ=255)
                                                                                                                            tCGGCCTTGGCCGGCTCATCCAGCCCCATGGCATGCAAACGGGCATAATGGCCATTAGCCGCAAGCAGCTCGGTGTGAGTACCGCGCTCGACCAGGCGGCCCTGGTCCATGACAAGGATCTGGTCAGCCTTCTCGa         >  5:346144/1‑136 (MQ=255)
                                                                                                                             cGGCCTTGGCCGGCTCATCCAGCCCCATGGCATGCAAACGGGCATAATGGCCATTAGCCGCAAGCAGCTCGGTGTGAGTACCGCGCTCGACCAGGCGGCCCTGGTCCATGACAAGGCTCTGGTCAGCCTTCTCGAt        >  5:295925/1‑136 (MQ=255)
                                                                                                                              ggCCTTGGCCGGCTCATCCAGCCCCATGGCATGCa                                                                                                            <  6:300292/35‑1 (MQ=255)
                                                                                                                              ggCCTTGGCCGGCTCATCCAGCCCCATGGCATGCa                                                                                                            >  5:300292/1‑35 (MQ=255)
                                                                                                                              ggCCTTGGCCGGCTCATCCAGCCCCATGGCATGCAAACGGGCATAATGGCCATTAGCCGCAAGCAGCTCGGAGAGAGTACCGCGCTCGACCAGGCGGCCCTGGTCGATGACAAGTATCTGGTCAGCCTTCTCGATg       >  2:117595/1‑136 (MQ=255)
                                                                                                                                   tGGCCGGCTCATCCAGCCCCATGGCATGCAAACGGGCATAATGGCCATTAGCCGCAAGCAGCTCGGTGTGAGTACCGCGCTCGACCAGGCGGCCCTGGTCCATGACAAGGATCTGGTCAGCCTTCTCGATGGTCGa  >  3:137057/1‑136 (MQ=255)
                                                                                                                                      |                                                                                                                                    
AATAATGAACAGGGGCAGGATATTAGCACAGGGTAGTCAATGGCTTTATGGCCAGATCATCCCTGGAAAATTCCCGTGACAATCGAGGCCATGACGGCCCCGATCAAGGAGGGTTCAGGTGATATCGGCCTTGGCCGGCTCATCCAGCCCCATGGCATGCAAACGGGCATAATGGCCATTAGCCGCAAGCAGCTCGGTGTGAGTACCGCGCTCGACCAGGCGGCCCTGGTCCATGACAAGGATCTGGTCAGCCTTCTCGATGGTCGA  >  ACB143/5612726‑5612992

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑