Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB143	5,693,175	A→C	43.8%	S236R (AGC→CGC)	pyrC' →	Dihydroorotase‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB143	5,693,175	0	A	C	43.8%	20.1 / 15.8	62	S236R (AGC→CGC)	pyrC'	Dihydroorotase‑like protein
Reads supporting (aligned to +/- strand):  ref base A (14/19);  new base C (11/17);  total (25/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGGCCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGC‑TGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCAGCC  >  ACB143/5693041‑5693308                                                                                                                                        |                                                                                                                                      tGGCCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGC‑TGGTGGAACAGAGCGGCGTGCGGGCGCACATCCGCCACATACCCAg                                                                                                                                      >  3:157338/1‑136 (MQ=255)      cACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGC‑TGGTGGAACAGAGCGGCGTGCGTGGGCCCGTCAGCCCGATAACCAGCGccc                                                                                                                                 >  5:319702/1‑136 (MQ=255)           aGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGC‑TGGTGGAACAGAGCGGCGTGCGTGCGCCCGTCAGCCAGATCACCAGCGCCCGTGgc                                                                                                                            >  1:234859/1‑136 (MQ=255)            ggTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGC‑TGGTGGAACAGAGCGGCGTGCGGGCGCCCTTCAGCCCGATCACCACCGCCCGTGgcg                                                                                                                           >  7:71718/1‑136 (MQ=255)                ccATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCACCCTGCTGC‑TGGTGGAACAGAGCGGCGGGGGGGGGCCCTTCCGCCCCGTCACCCGCCCCCGGGGcgcgcg                                                                                                                       >  4:85623/1‑136 (MQ=255)                ccATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGC‑TGGTGGAACAGAGCGGCGTGGGTGCGCAGATCAGCCAGATAAACCCCGCCCGGGGcgcgcg                                                                                                                       >  6:95679/1‑136 (MQ=255)                ccATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGC‑TGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGcgcgcg                                                                                                                       >  8:118491/1‑136 (MQ=255)                 cATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGC‑TGGTGGAACAGAGCGGCGTGCGTGGGCACATAAACCCGAAAAACACCGCCCGTGgcgcgccg                                                                                                                      >  4:178705/1‑134 (MQ=255)                 cATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGC‑TGGTGGAACAGAGAGGCGTGCGGGCGCGCATGAGACAGATCAACCGCCCCCGTGgcgcccca                                                                                                                      >  2:77925/1‑132 (MQ=255)                       cAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCGGGCGCGCAACCTGCTGC‑TGGTGGACCAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGGGGCGCGCGGCTGATc                                                                                                                <  8:128220/136‑1 (MQ=255)                       cAGCTTCCTCGGCCGGCCGGGCATCCCGGAGAGCGCCTAAACCGTGGCCCTGGCGCGCAACCTGCTGC‑TGGTGGAACAGCGCGGCGTGCGGGCGCACTTCAGCCATATCACCAGCGCCCGTGGCGCGCGGCTGATc                                                                                                                <  3:247480/136‑1 (MQ=255)                        aGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGC‑TGGTGGATCAGAGCGGCGGGCGGGGGCCCGTCAGCCAGCTGCCCAGCCCCCGGGGCGCGCGGcggctag                                                                                                               >  5:318228/1‑130 (MQ=255)                             ccTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGC‑TGGTGGAACAGAGCGGCGTGCGGGGGGCCTTCAGCCCGATCACCCCCGCCCGTGGCGCGCGGCGGCTCgccccg                                                                                                          >  7:58435/1‑134 (MQ=255)                              ctgggCGTGCCGGGCAGCCCGGAGCGCGCCTAAACCGGGGCCCTGGCGCGAAACCTGCTGC‑TGGGGTAACCGCGCGGCGGGCGGGCGCACTTCCGCCATACCCCCCGCGCCCGGGGCGCGCGGCTGATCgcccagg                                                                                                         <  8:372233/133‑1 (MQ=255)                              cTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGC‑TGGTGGAACAGAGCGGCGTGCGTGCGAACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCgcccagg                                                                                                         <  5:196037/136‑1 (MQ=255)                              cTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGC‑TGGTGGAACAGAGCGGCGGGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCgcccagg                                                                                                         <  2:125293/136‑1 (MQ=255)                                  gCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGC‑TGGTGGAACAGAGCGGCGTGCGTGGGCCCTTCAGCCCGCTCACCCGCGCCCGTGGCGCGCGGCGGATCgcccaggcc                                                                                                       >  1:48431/1‑134 (MQ=255)                                  gCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGC‑TGGTGGAACAGAGCGGCGTGCGTGCGCACATCAGCCAGATCACCCCCCCCCGTGGCGCGCGGCTGCTAgccccggcccc                                                                                                     >  6:303693/1‑135 (MQ=255)                                  gCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGC‑TGGTGGAACAGAGCGGCGTGCGGGCGCACATCAGGCACAGAACCCACCCCCGGGGGGCGCGGGGGCTCgcccacgcgca                                                                                                     >  2:207625/1‑136 (MQ=255)                                         gggCATCCCGGAGAGCGCCGAAACCGTGGCCCGGGCGCGCAACCTGCTGC‑GGGTGGACCAGAGCGGCGTGCGGGCGCACTTCAGCCAGCTCACCAGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTg                                                                                              <  5:382688/136‑1 (MQ=255)                                         gggCAGCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGC‑TGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGAGCACCCGCGCCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTg                                                                                              <  1:339505/136‑1 (MQ=255)                                                       gcgcCTAAACGGGGGCCCGGGCGCGCAACCTGCTGC‑TGGTGGAACCGCGGGGGGGGGGTGCGCAATTCACCCACATCCCCCCCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAc                                                                                <  7:154438/136‑1 (MQ=255)                                                       gcgcCTAAACCGTGGCCCGGGCGCGCAACCTGCTGG‑TGGGGCAACAGCGCGGGGGGCGGGCCCACTTCAGCCACACCCCCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAc                                                                                <  6:211661/136‑1 (MQ=255)                                                       gcgcCGAAACCGTGGCCCTGGCGCGCAACCTGCTGC‑TGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAc                                                                                <  7:82271/136‑1 (MQ=255)                                                        ggcCGAACCCGTGGCCCTGGCGCGCAACCTGCGGC‑TGGTGGAACAGAGCGGCGTGCGTGCGCATTTCAGCCAGAGCACCAGCGCCGGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAcc                                                                               <  7:247526/135‑1 (MQ=255)                                                            gaaccggTGGCCCGGGCGCGCACCCGGCTGG‑TGGTGGACCCGCGCGGGGGGCGTGCGCACTGCCCCCCGACCCCCCGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAccgccg                                                                           <  1:206840/130‑1 (MQ=255)                                                            gAAACGGTGGCCCGGGCGCGCAACCTGCTGC‑GGGTGGAACAGCGCGGCGTGCGGGCGCACTTCAGCCATATCACCAGCGCCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAccgccg                                                                           <  7:206598/136‑1 (MQ=255)                                                            gAAACCGTGGCCCTGGCGCGCAACCTGCTGC‑TGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAccgccg                                                                           <  7:118491/136‑1 (MQ=255)                                                            gAAACCGTGGCCCTGGCGCGCAACCTGCTGC‑TGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAccgccg                                                                           <  5:183900/136‑1 (MQ=255)                                                              aaCCGTGGCCCTGGCGCGAAACCTGCTGC‑TGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGa                                                                          <  2:334858/135‑1 (MQ=255)                                                                ccGTGGCCCTGGCGCGCAACCTGCTGC‑TGGTGGAACAGAGCGGCGTGCGGGGGGCCCTAAGCCCGCAAACCCACCCCCCGGGCGCGCCGCCGGTCGCCCCCGCCCCCGCCGTGGGCCGGCCGGGGAcccccgcggg                                                                       >  2:167595/1‑132 (MQ=255)                                                                 ggTGGCCCTGGCGCGCAACCTGCTGC‑TGGTTTACCATAGGGGCGGGGGTGCGCACTTCACCCATATACCCCCCCCCGGTGGGGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                                      <  6:389901/135‑1 (MQ=255)                                                                 cggggCCCTGGCGCGCAACCTGCTGC‑TGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCCGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                                      <  8:317746/133‑1 (MQ=255)                                                                 cgggccccGGGCGCGCAACCGGCGGC‑TGGGGCAACCTCCCGGGGGGCGGGCGCACTTCAGCCCGACCCCCCGCGCCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                                      <  2:396533/131‑1 (MQ=255)                                                                 cGTGGCCCTGGCGCGCAACGTGCTGC‑GGGTGGAACTGAGCGGCGGGCGTGCGCACTTCAGCCATATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                                      <  4:304723/136‑1 (MQ=255)                                                                 cGTGGCCCTGGCGCGCAACCTGCTGC‑TGGGGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                                      <  7:65981/136‑1 (MQ=255)                                                                 cGTGGCCCTGGCGCGAAACCTGCTGC‑TGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                                      <  3:93616/136‑1 (MQ=255)                                                                      cccTGGCGCGCAACCTGCTGC‑TGGTGGAACAGAGCGGCGTGCGTGCGCACGTCAGCCACATAACCACCCCCCGTTGCGCCCCGCGGCACGCCCCCGCCCACGCCGTGGGGCTGCCGGTAAACCCCGATTTAtcg                                                                   >  8:287091/1‑134 (MQ=255)                                                                          ggCGCGAAACCGCCTGC‑TGGTGCAACCTCGCGGGGTGCTTGCGAACTCCTCCCCTATCACCCCCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTAc                                                             <  8:324670/136‑1 (MQ=255)                                                                           gcgcgcAACCTGCTGC‑TGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCCGGCCTTGGGCCTGCCGGGAACCGCCGATGTAGCGCTGTAcc                                                            >  1:256237/1‑136 (MQ=255)                                                                              cgaacccTGCTGCGTGGT‑GAACATAGCGGCTTGCGGGCGCACTTCACCCAGACCACCCGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGc                                                         <  4:303250/131‑1 (MQ=255)                                                                                cAACCTGCTGC‑TGGTGGAACAGAGCGGCGTGCGGGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTg                                                       <  1:351070/136‑1 (MQ=255)                                                                                  aCCTGGTGG‑GGGTGCAACAGCGGGGGGTGCGGGCGCATTCCAGCCAGAGCCCCCGGGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGAt                                                     <  4:290884/136‑1 (MQ=255)                                                                                                        gcggggGGCGGGCGCACTCCCGCCCCACCCCCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTgcg                                <  6:2826/136‑1 (MQ=255)                                                                                                         cggggTGCGTGCCCACTTCCCCCACATCCCCAGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTgcgc                               <  5:14696/136‑1 (MQ=255)                                                                                                          ggcgTGCGTGCGCACTTCAGCCAGATCACCCCCGCCCCTGGCGCGCGGCTGATCGCCCCGGCCCCCGCCGTGGGCCTGCCCGGAACCGCCGCTGTAGCGCTGTACCCCCTGATCCTCACGGGAGAGTCGCTGcgcg                              >  8:60286/1‑136 (MQ=255)                                                                                                          ggcgTGCGGGCGCACTTCCGCCCGCTCACCCGCGCCCGGGGCGCGCGGCTGATCGCCCCCGCCCCGGCCTTGGGCCTGCCGGTAACCGCCCCTGTAGCGCTGTACCAGCTGCTACTCACGGCCGCGTCgcggcggg                              >  6:190152/1‑134 (MQ=255)                                                                                                            cgtgcgtgCGCCCGTAAGCCAGATCACCCGCCCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCCGGAACCGCCGATGTAGCGCGGTACCAGCTGATACGCACGGGCGCGTCGCGGcgcgcg                            >  4:261488/1‑134 (MQ=255)                                                                                                             gtgcgtgcGCACTGCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt                           <  8:83605/136‑1 (MQ=255)                                                                                                             gtgcgggcGCACTTCAGCCAGATCACCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt                           <  6:130176/136‑1 (MQ=255)                                                                                                             gggggtgcgCAGTTCACCATAACCACCCGCGCCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt                           <  2:357631/132‑1 (MQ=255)                                                                                                             gggggtgcgAAGTTCCGCCAGATCACCCGCGCCGGGGGGGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt                           <  6:319702/132‑1 (MQ=255)                                                                                                             gggcgtgcgCACTTCCCCCATAACCCCCGGGCCGGTGGGGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt                           <  3:92051/134‑1 (MQ=255)                                                                                                             gggcgggcgCACTTCCGCCAGACCCCCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt                           <  3:177453/134‑1 (MQ=255)                                                                                                               gcgtgcgCACTTAAGCCCGATCACCAGCCCCCGTGGCGCGCGGCTGATAGCCCAGGCCCAGGCCTTGGGCCTGCCGGGAACCGCCGATGTAGCGCGGTACCACCAGCTCCTCCCGGCCGAGTCGCTGCGCGAGGtc                         >  6:297875/1‑136 (MQ=255)                                                                                                                  tgcgCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTcc                      <  7:110464/136‑1 (MQ=255)                                                                                                                                    cACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCAg    >  7:96965/1‑136 (MQ=255)                                                                                                                                    cACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCAg    >  5:151046/1‑136 (MQ=255)                                                                                                                                    cACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGAGCGAGTTCTCCAGCCTGTACCACGTGCAg    <  2:75428/136‑1 (MQ=255)                                                                                                                                     aCCTGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGAGCGAGTTCTCCAGCCTGTACCACGTGCAg    <  2:75422/135‑1 (MQ=255)                                                                                                                                     aCCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCAgc   >  8:149807/1‑136 (MQ=255)                                                                                                                                      ccAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCAgcc  >  1:334039/1‑136 (MQ=255)                                                                                                                                        |                                                                                                                                      TGGCCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGC‑TGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCAGCC  >  ACB143/5693041‑5693308

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑