Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 5,693,183 T→G 33.8% R238R (CGT→CGG)  pyrC' → Dihydroorotase‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1435,693,1830TG33.8% 44.4 / 9.2 61R238R (CGT→CGG) pyrC'Dihydroorotase‑like protein
Reads supporting (aligned to +/- strand):  ref base T (18/21);  new base G (6/16);  total (24/37)
Fisher's exact test for biased strand distribution p-value = 1.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGC‑TGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCAGCCGCCGCTGCG  >  ACB143/5693051‑5693317
                                                                                                                                     |                                                                                                                                      
aGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGC‑TGGTGGAACAGAGCGGCGTGCGTGCGCCCGTCAGCCAGATCACCAGCGCCCGTGgc                                                                                                                                     >  1:234859/1‑136 (MQ=255)
 ggTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGC‑TGGTGGAACAGAGCGGCGTGCGGGCGCCCTTCAGCCCGATCACCACCGCCCGTGgcg                                                                                                                                    >  7:71718/1‑136 (MQ=255)
     ccATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCACCCTGCTGC‑TGGTGGAACAGAGCGGCGGGGGGGGGCCCTTCCGCCCCGTCACCCGCCCCCGGGGcgcgcg                                                                                                                                >  4:85623/1‑136 (MQ=255)
     ccATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGC‑TGGTGGAACAGAGCGGCGTGGGTGCGCAGATCAGCCAGATAAACCCCGCCCGGGGcgcgcg                                                                                                                                >  6:95679/1‑136 (MQ=255)
     ccATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGC‑TGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGcgcgcg                                                                                                                                >  8:118491/1‑136 (MQ=255)
      cATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGC‑TGGTGGAACAGAGCGGCGTGCGTGGGCACATAAACCCGAAAAACACCGCCCGTGgcgcgccg                                                                                                                               >  4:178705/1‑134 (MQ=255)
      cATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGC‑TGGTGGAACAGAGAGGCGTGCGGGCGCGCATGAGACAGATCAACCGCCCCCGTGgcgcccca                                                                                                                               >  2:77925/1‑132 (MQ=255)
            cAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCGGGCGCGCAACCTGCTGC‑TGGTGGACCAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGGGGCGCGCGGCTGATc                                                                                                                         <  8:128220/136‑1 (MQ=255)
            cAGCTTCCTCGGCCGGCCGGGCATCCCGGAGAGCGCCTAAACCGTGGCCCTGGCGCGCAACCTGCTGC‑TGGTGGAACAGCGCGGCGTGCGGGCGCACTTCAGCCATATCACCAGCGCCCGTGGCGCGCGGCTGATc                                                                                                                         <  3:247480/136‑1 (MQ=255)
             aGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGC‑TGGTGGATCAGAGCGGCGGGCGGGGGCCCGTCAGCCAGCTGCCCAGCCCCCGGGGCGCGCGGcggctag                                                                                                                        >  5:318228/1‑130 (MQ=255)
                  ccTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGC‑TGGTGGAACAGAGCGGCGTGCGGGGGGCCTTCAGCCCGATCACCCCCGCCCGTGGCGCGCGGCGGCTCgccccg                                                                                                                   >  7:58435/1‑134 (MQ=255)
                   ctgggCGTGCCGGGCAGCCCGGAGCGCGCCTAAACCGGGGCCCTGGCGCGAAACCTGCTGC‑TGGGGTAACCGCGCGGCGGGCGGGCGCACTTCCGCCATACCCCCCGCGCCCGGGGCGCGCGGCTGATCgcccagg                                                                                                                  <  8:372233/133‑1 (MQ=255)
                   cTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGC‑TGGTGGAACAGAGCGGCGTGCGTGCGAACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCgcccagg                                                                                                                  <  5:196037/136‑1 (MQ=255)
                   cTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGC‑TGGTGGAACAGAGCGGCGGGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCgcccagg                                                                                                                  <  2:125293/136‑1 (MQ=255)
                       gCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGC‑TGGTGGAACAGAGCGGCGTGCGTGGGCCCTTCAGCCCGCTCACCCGCGCCCGTGGCGCGCGGCGGATCgcccaggcc                                                                                                                >  1:48431/1‑134 (MQ=255)
                       gCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGC‑TGGTGGAACAGAGCGGCGTGCGTGCGCACATCAGCCAGATCACCCCCCCCCGTGGCGCGCGGCTGCTAgccccggcccc                                                                                                              >  6:303693/1‑135 (MQ=255)
                       gCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGC‑TGGTGGAACAGAGCGGCGTGCGGGCGCACATCAGGCACAGAACCCACCCCCGGGGGGCGCGGGGGCTCgcccacgcgca                                                                                                              >  2:207625/1‑136 (MQ=255)
                              gggCATCCCGGAGAGCGCCGAAACCGTGGCCCGGGCGCGCAACCTGCTGC‑GGGTGGACCAGAGCGGCGTGCGGGCGCACTTCAGCCAGCTCACCAGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTg                                                                                                       <  5:382688/136‑1 (MQ=255)
                              gggCAGCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGC‑TGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGAGCACCCGCGCCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTg                                                                                                       <  1:339505/136‑1 (MQ=255)
                                            gcgcCTAAACGGGGGCCCGGGCGCGCAACCTGCTGC‑TGGTGGAACCGCGGGGGGGGGGTGCGCAATTCACCCACATCCCCCCCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAc                                                                                         <  7:154438/136‑1 (MQ=255)
                                            gcgcCTAAACCGTGGCCCGGGCGCGCAACCTGCTGG‑TGGGGCAACAGCGCGGGGGGCGGGCCCACTTCAGCCACACCCCCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAc                                                                                         <  6:211661/136‑1 (MQ=255)
                                            gcgcCGAAACCGTGGCCCTGGCGCGCAACCTGCTGC‑TGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAc                                                                                         <  7:82271/136‑1 (MQ=255)
                                             ggcCGAACCCGTGGCCCTGGCGCGCAACCTGCGGC‑TGGTGGAACAGAGCGGCGTGCGTGCGCATTTCAGCCAGAGCACCAGCGCCGGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAcc                                                                                        <  7:247526/135‑1 (MQ=255)
                                                 gaaccggTGGCCCGGGCGCGCACCCGGCTGG‑TGGTGGACCCGCGCGGGGGGCGTGCGCACTGCCCCCCGACCCCCCGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAccgccg                                                                                    <  1:206840/130‑1 (MQ=255)
                                                 gAAACGGTGGCCCGGGCGCGCAACCTGCTGC‑GGGTGGAACAGCGCGGCGTGCGGGCGCACTTCAGCCATATCACCAGCGCCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAccgccg                                                                                    <  7:206598/136‑1 (MQ=255)
                                                 gAAACCGTGGCCCTGGCGCGCAACCTGCTGC‑TGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAccgccg                                                                                    <  5:183900/136‑1 (MQ=255)
                                                 gAAACCGTGGCCCTGGCGCGCAACCTGCTGC‑TGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAccgccg                                                                                    <  7:118491/136‑1 (MQ=255)
                                                   aaCCGTGGCCCTGGCGCGAAACCTGCTGC‑TGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGa                                                                                   <  2:334858/135‑1 (MQ=255)
                                                     ccGTGGCCCTGGCGCGCAACCTGCTGC‑TGGTGGAACAGAGCGGCGTGCGGGGGGCCCTAAGCCCGCAAACCCACCCCCCGGGCGCGCCGCCGGTCGCCCCCGCCCCCGCCGTGGGCCGGCCGGGGAcccccgcggg                                                                                >  2:167595/1‑132 (MQ=255)
                                                      ggTGGCCCTGGCGCGCAACCTGCTGC‑TGGTTTACCATAGGGGCGGGGGTGCGCACTTCACCCATATACCCCCCCCCGGTGGGGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                                               <  6:389901/135‑1 (MQ=255)
                                                      cggggCCCTGGCGCGCAACCTGCTGC‑TGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCCGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                                               <  8:317746/133‑1 (MQ=255)
                                                      cgggccccGGGCGCGCAACCGGCGGC‑TGGGGCAACCTCCCGGGGGGCGGGCGCACTTCAGCCCGACCCCCCGCGCCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                                               <  2:396533/131‑1 (MQ=255)
                                                      cGTGGCCCTGGCGCGCAACGTGCTGC‑GGGTGGAACTGAGCGGCGGGCGTGCGCACTTCAGCCATATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                                               <  4:304723/136‑1 (MQ=255)
                                                      cGTGGCCCTGGCGCGCAACCTGCTGC‑TGGGGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                                               <  7:65981/136‑1 (MQ=255)
                                                      cGTGGCCCTGGCGCGAAACCTGCTGC‑TGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                                               <  3:93616/136‑1 (MQ=255)
                                                           cccTGGCGCGCAACCTGCTGC‑TGGTGGAACAGAGCGGCGTGCGTGCGCACGTCAGCCACATAACCACCCCCCGTTGCGCCCCGCGGCACGCCCCCGCCCACGCCGTGGGGCTGCCGGTAAACCCCGATTTAtcg                                                                            >  8:287091/1‑134 (MQ=255)
                                                               ggCGCGAAACCGCCTGC‑TGGTGCAACCTCGCGGGGTGCTTGCGAACTCCTCCCCTATCACCCCCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTAc                                                                      <  8:324670/136‑1 (MQ=255)
                                                                gcgcgcAACCTGCTGC‑TGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCCGGCCTTGGGCCTGCCGGGAACCGCCGATGTAGCGCTGTAcc                                                                     >  1:256237/1‑136 (MQ=255)
                                                                   cgaacccTGCTGCGTGGT‑GAACATAGCGGCTTGCGGGCGCACTTCACCCAGACCACCCGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGc                                                                  <  4:303250/131‑1 (MQ=255)
                                                                     cAACCTGCTGC‑TGGTGGAACAGAGCGGCGTGCGGGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTg                                                                <  1:351070/136‑1 (MQ=255)
                                                                       aCCTGGTGG‑GGGTGCAACAGCGGGGGGTGCGGGCGCATTCCAGCCAGAGCCCCCGGGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGAt                                                              <  4:290884/136‑1 (MQ=255)
                                                                                             gcggggGGCGGGCGCACTCCCGCCCCACCCCCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTgcg                                         <  6:2826/136‑1 (MQ=255)
                                                                                              cggggTGCGTGCCCACTTCCCCCACATCCCCAGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTgcgc                                        <  5:14696/136‑1 (MQ=255)
                                                                                               ggcgTGCGTGCGCACTTCAGCCAGATCACCCCCGCCCCTGGCGCGCGGCTGATCGCCCCGGCCCCCGCCGTGGGCCTGCCCGGAACCGCCGCTGTAGCGCTGTACCCCCTGATCCTCACGGGAGAGTCGCTGcgcg                                       >  8:60286/1‑136 (MQ=255)
                                                                                               ggcgTGCGGGCGCACTTCCGCCCGCTCACCCGCGCCCGGGGCGCGCGGCTGATCGCCCCCGCCCCGGCCTTGGGCCTGCCGGTAACCGCCCCTGTAGCGCTGTACCAGCTGCTACTCACGGCCGCGTCgcggcggg                                       >  6:190152/1‑134 (MQ=255)
                                                                                                 cgtgcgtgCGCCCGTAAGCCAGATCACCCGCCCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCCGGAACCGCCGATGTAGCGCGGTACCAGCTGATACGCACGGGCGCGTCGCGGcgcgcg                                     >  4:261488/1‑134 (MQ=255)
                                                                                                  gtgcgtgcGCACTGCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt                                    <  8:83605/136‑1 (MQ=255)
                                                                                                  gtgcgggcGCACTTCAGCCAGATCACCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt                                    <  6:130176/136‑1 (MQ=255)
                                                                                                  gggggtgcgCAGTTCACCATAACCACCCGCGCCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt                                    <  2:357631/132‑1 (MQ=255)
                                                                                                  gggggtgcgAAGTTCCGCCAGATCACCCGCGCCGGGGGGGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt                                    <  6:319702/132‑1 (MQ=255)
                                                                                                  gggcgtgcgCACTTCCCCCATAACCCCCGGGCCGGTGGGGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt                                    <  3:92051/134‑1 (MQ=255)
                                                                                                  gggcgggcgCACTTCCGCCAGACCCCCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt                                    <  3:177453/134‑1 (MQ=255)
                                                                                                    gcgtgcgCACTTAAGCCCGATCACCAGCCCCCGTGGCGCGCGGCTGATAGCCCAGGCCCAGGCCTTGGGCCTGCCGGGAACCGCCGATGTAGCGCGGTACCACCAGCTCCTCCCGGCCGAGTCGCTGCGCGAGGtc                                  >  6:297875/1‑136 (MQ=255)
                                                                                                       tgcgCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTcc                               <  7:110464/136‑1 (MQ=255)
                                                                                                                         cACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCAg             >  7:96965/1‑136 (MQ=255)
                                                                                                                         cACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCAg             >  5:151046/1‑136 (MQ=255)
                                                                                                                         cACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGAGCGAGTTCTCCAGCCTGTACCACGTGCAg             <  2:75428/136‑1 (MQ=255)
                                                                                                                          aCCTGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGAGCGAGTTCTCCAGCCTGTACCACGTGCAg             <  2:75422/135‑1 (MQ=255)
                                                                                                                          aCCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCAgc            >  8:149807/1‑136 (MQ=255)
                                                                                                                           ccAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCAgcc           >  1:334039/1‑136 (MQ=255)
                                                                                                                                    gTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGGTCTCCAGCCTGTACCCCGTGCAGCCGCCGCAgcg  >  2:115720/1‑136 (MQ=255)
                                                                                                                                     |                                                                                                                                      
AGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGC‑TGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCAGCCGCCGCTGCG  >  ACB143/5693051‑5693317

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑