Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 535,788 C→T 9.1% S326S (TCC→TCT)  rpoC → DNA‑directed RNA polymerase subunit beta'

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB143535,7880CT 9.1% 136.6 / 4.1 44S326S (TCC→TCT) rpoCDNA‑directed RNA polymerase subunit beta'
Reads supporting (aligned to +/- strand):  ref base C (21/19);  new base T (2/2);  total (23/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.12e-01

GCTGGATCTGTCGGCGCCGGACATCATCGTGCGCAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTACCGTAGGTCCGACCCTGCGTCTGCACCAGTGCGGTCTGCCGAAGAA  >  ACB143/535656‑535922
                                                                                                                                    |                                                                                                                                      
gctgGATCTGTCGGCGCCGGACATCATCGTGCGCAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTg                                                                                                                                     >  5:234672/1‑136 (MQ=255)
    gagcTGTCGGCGCCGGACATCATCGTGCGCAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGcc                                                                                                                                  <  8:327666/132‑1 (MQ=255)
        tGTCGGCGCCGGACATCATCGTGCGCAACGAAAAGCGCATGCTGCAGGAAGCGGTTGAGGCTCTGCTGGACAACGGCCGTCGTTGTCGCGGCATCACTCTCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACaa                                                                                                                             >  6:364067/1‑135 (MQ=255)
          tCGGCGCCGGACATCATCGTGCGCAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATg                                                                                                                            <  6:13023/135‑1 (MQ=255)
             gcgcCGGACATCATCGTGCGCAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATc                                                                                                                         >  6:372693/1‑135 (MQ=255)
                   gACATCATCGTGCGCAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTa                                                                                                                  <  1:378719/136‑1 (MQ=255)
                       tcatcGTGCGCAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGc                                                                                                               >  2:338452/1‑135 (MQ=255)
                                gtaaCGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGCGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGtt                                                                                                      >  5:154154/3‑135 (MQ=255)
                                gcAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGttt                                                                                                     >  8:106544/1‑136 (MQ=255)
                                  aaCGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTcc                                                                                                   >  2:47387/1‑136 (MQ=255)
                                     gAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGt                                                                                                 >  6:338293/1‑135 (MQ=255)
                                            gcATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACtt                                                                                         <  4:238496/136‑1 (MQ=255)
                                                 ctgcAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCg                                                                                    >  8:313478/1‑136 (MQ=255)
                                                 ctgcAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCg                                                                                    >  8:185116/1‑136 (MQ=255)
                                                  tgcAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCgg                                                                                   >  7:53628/1‑136 (MQ=255)
                                                      ggAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGGCGCGCCATCACTGGCTCGAACAAGCGGCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAg                                                                               <  1:329699/136‑1 (MQ=255)
                                                      ggAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGCGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAg                                                                               <  6:154154/136‑1 (MQ=255)
                                                           cGGTTGACGGTCTGCTGGACAACGGCCGTCGTGTTCGCGCCATCAGTGGCTCGAACAAGCGTCCTCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCgtgt                                                                          <  2:365194/136‑1 (MQ=255)
                                                            ggTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGtt                                                                         <  6:73884/136‑1 (MQ=255)
                                                                 aCGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGacta                                                                    >  7:284672/1‑136 (MQ=255)
                                                                    ctctGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTc                                                                 <  7:267975/136‑1 (MQ=255)
                                                                    ctctGCTGGACAACGGCCGACGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCAGAAGTCCCTGCCCGACATGATCAAAGGTAACCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTc                                                                 >  4:344614/1‑136 (MQ=255)
                                                                          tgGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCg                                                           >  5:336752/1‑136 (MQ=255)
                                                                           ggACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGt                                                          >  6:33519/1‑136 (MQ=255)
                                                                                aCGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGt                                                     <  1:250916/136‑1 (MQ=255)
                                                                                aCGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGt                                                     <  5:114285/136‑1 (MQ=255)
                                                                                    ccGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACAt                                                                                                                             <  5:94269/60‑1 (MQ=255)
                                                                                    ccGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACAt                                                                                                                             >  6:94269/1‑60 (MQ=255)
                                                                                    ccGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAAtt                                                 >  2:111188/1‑136 (MQ=255)
                                                                                    ccGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAAtt                                                 <  3:116562/136‑1 (MQ=255)
                                                                                     cgtcgtGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATTATCAAAGGAAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTa                                                <  7:119722/136‑1 (MQ=255)
                                                                                      gtcgtGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTAc                                               <  6:234672/136‑1 (MQ=255)
                                                                                      gtcgtGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTAc                                               >  7:355877/1‑136 (MQ=255)
                                                                                      gtcgcGGTCGTGCGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATCAc                                               <  7:357135/136‑1 (MQ=255)
                                                                                            gTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTACCGTAgg                                         <  8:112244/136‑1 (MQ=255)
                                                                                                    aTCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTACCGTAGGTCCGAccc                                 >  8:50830/1‑136 (MQ=255)
                                                                                                    aTCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTACCGTAGGTCCGAccc                                 <  2:283272/136‑1 (MQ=255)
                                                                                                       aCTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTACCGTAGGTCCGACCCTg                               <  3:338577/135‑1 (MQ=255)
                                                                                                              ctaaCAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATCACCGTAGGTCCATACCTGCGTCTGCa                       >  1:169073/3‑136 (MQ=255)
                                                                                                                   aaGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTACCGTAGGTCCGACCCTGCGTCTGCACCAGt                  >  2:118955/1‑136 (MQ=255)
                                                                                                                        tCCGCTGAAGTCCCTTGACGACATGATCAAAGGTAAGCAAGTGCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTACCGTAGGTCCGACCCTGCGTCTGCACCAGTGCGGt             <  6:336752/136‑1 (MQ=255)
                                                                                                                           gCTGAAGTCCCTGGCCGACATTATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTACCGTAGGTCCGACCCTGCGTCTGCACCAGTGCGGTCTg          <  1:395610/136‑1 (MQ=255)
                                                                                                                             tGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTACCGTAGGTCCGACCCTGCGTCTGCACCAGTGCGGTCTGcc        >  5:239166/1‑136 (MQ=255)
                                                                                                                                  tCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTACCGTAGGTCCGACCCTGCGTCTGCACCAGTGCGGTCTGCCgaag    >  8:208326/1‑135 (MQ=255)
                                                                                                                                    ccTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTACCGTAGGTCCGACCCTGCGTCTGCACCAGTGCGGTCTGCCgaagaa  <  5:33827/135‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                      
GCTGGATCTGTCGGCGCCGGACATCATCGTGCGCAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTACCGTAGGTCCGACCCTGCGTCTGCACCAGTGCGGTCTGCCGAAGAA  >  ACB143/535656‑535922

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑