Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 740,674 T→G 29.2% L38R (CTG→CGG)  CJ019_00704 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB143740,6740TG29.2% 56.7 / 2.9 50L38R (CTG→CGG) CJ019_00704hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (24/10);  new base G (10/6);  total (34/16)
Fisher's exact test for biased strand distribution p-value = 7.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCATGTAGGAGGATCTCCGTATGAGAACGGTTTTGTTGTGTCTTGGGCTTGCAAGTGCAGGCTTCTGTGTGGGCTCTCACTGGCCGACACCACTCTGGGTCATCGTCGTGTTGTGGGTGGTGGGGGGGGCGCTGTGGGTTCTGGCTGAATGGCTGGAGCGGAGGGGGCGCAATCAATGATGCGCGCCAATCTAGACGAGCTGGTGATCGTGAGCTGTTTGTTTCCAGCTATGAGGTGGTCAGCTTCTATTGCGCGATCCGTGCTCA  >  ACB143/740542‑740807
                                                                                                                                    |                                                                                                                                     
ccATGTAGGAGGATCTCCGTATGAGAACGGTTTTGTTGTGTCTTGGGCTTGCAAGTGCAGGCTTCTGTGTGGGCTCTCACTGGCCGACACCACTCTGGGTCATCGTCGTGTTGTGGGTGGGGGGGGGGGCGCtgtg                                                                                                                                    >  5:78745/1‑136 (MQ=255)
 cATGTAGTTTGATCTCGGTTTGAGAATGTTTTTGTGTTGTTTTTGGCTTTCAAGTGCTGGCTTTTGTGTGGGCGCTCACTGGCCTACACCACTCTGGGTCATCGTCGTGTTGTGGGTGGTGGGGGGGGCGCTGTgg                                                                                                                                   <  4:3693/136‑1 (MQ=255)
 cATGTAGGAGGTTCTCCGTATGAGAACGGTTTTGTTGTGTCTTGGGCTTGCAAGTGCAGGATTCTTGGTGGGCTCTCACTGGCCGACACCACTCTGGGTCATCGTCGTGTTGTGGGTGGTGGGGGGGGCGCTGTgg                                                                                                                                   <  8:321427/136‑1 (MQ=255)
      aggaggATCTCCGTATGAGAACGGTTTTGTTGTGTCTTGGGCTTGCAAGTGCAGGCTTCTGTGTGGGCTCTCACTGGCCGACACCACTCTGGGTCATCGTCGTGGTGTGGGTGGTGGGGGGGGCGCTGTggggtgt                                                                                                                              >  7:72949/1‑132 (MQ=255)
         cggaTCTCCGTATGATAACGGTTTTGTTGTGGCTGGGGCTTGCAAGTGCAGGCTTCTGTGTGGGCTCTCACTGGCCGACACCACTCTGGGTCATCGTCGTGTTGTGGGTGGTGGGGGGGGCGCTGTGGGTTCTGGc                                                                                                                           <  3:357962/135‑1 (MQ=255)
          ggaTCTCCGTATGAGAACGGTTTTGTTGTGTCTTGGGCTTGCAAGTGCAGGCTTCTGTGTGGGCTCTCACTGGCCGACACCACTCTGGGTCATCGTCGTGTTGTGGGTGGTGGGGGGGGCGCTGTGGGTTCTGGCt                                                                                                                          >  6:92693/1‑136 (MQ=255)
             tctcCGTATGAGAACGGTTTTGTTGTGTCTTGGGCTTGCAAGTGCAGGCTTCTGTGTGGGCTCTCACTGGCCGACACCACTCTGGGTCATCGTCGTGTTGTGGGTGGTGGGGGGGGCGCTGTGGGTTCTGGGTGaa                                                                                                                       >  3:9002/1‑136 (MQ=255)
             tctcCGTATGAGAACGGTTTTGTTGTGTCTTGGGCTTGCAAGTGCAGGCTTCTGTGTGGGCTCTCACTGGCCGACACCACTCTGGGTCATCGTCGTGGTGTGGGTGGTGGGGGGGGCGCGGTGGGTTGTGGCGGaa                                                                                                                       >  3:3766/1‑136 (MQ=255)
              ctcCGTATGAGAACGGTTTTGTTGTGTCTTGGGCTTGCAAGTGCAGGCTTCTGTGTGGGCTCTCACTGGCCGACACCACTCTGGGTCATCGTCGTGTTGTTGGTGGTGGGGGGGGCGCGGTGGGTTCTGGCTGAAt                                                                                                                      >  1:366128/1‑136 (MQ=255)
                ccGTATGAGAACGGTTTTGTTGTGTCTTGGGCTTGCAAGTGCAGGCTTCTGTGTGGGCTCTCACTGGCCGACACCACTCTGGGTCATCGTCGTGTTGTGGGTGGTGGGGGGGGCGCGGTGGGGTCTGGCTGAATgg                                                                                                                    >  4:371433/1‑136 (MQ=255)
                ccGTATGAGAACGGTTTTGTTGTGTCTTGGGCTTGCAAGTGCAGGCTTCTGTGTGGGCTCTCACTGGCCGACACCACTCTGGGTCATCGTCGTGTTGTGGGTGGGGGGGGGGGCGCGGTGGGGTGTGGGTGAATgg                                                                                                                    >  3:90846/1‑136 (MQ=255)
                ccGTATGAGAACGGTTTTGTTGTGTCTTGGGCTTGCAAGTGCAGGCTTCTGTGTGGGCTCTCACTGGCCGACACCACTCTGGGGCATCGTCGTGGTGTGGGTGGTGGGGGGGGCGCGGGGGGTTCTGGCTGAATgg                                                                                                                    >  1:346264/1‑136 (MQ=255)
                 cGTATGAGAACGGTTTTGTTGTGTCTTGGTCTTGCAAGTGCAGGCTTCTGTGTGGGCTCTCACTGGCCGACACCACTCTGGGTCATAGTCGTGTTGTGGGTGGTGGGGGGGGCGCTGTGGGTTGGGGCTGCATggg                                                                                                                   >  1:73356/1‑135 (MQ=255)
                  gTATGAGAACGGTTTTGTTGTGTCTTGGGCTTGCAAGTGCAGGCTTCTGTGTGGGCTCTCACTGGCCGACACCACTCTGGGTCATCGTCGTGTTGTGGGTGGTGGGGGGGGCGCTGTGGGTTCGGGCTGAATGGCt                                                                                                                  >  4:226629/1‑136 (MQ=255)
                          aCGGTTTTGTTGTGTCTTGGGCTTGCAAGTGCAGGCTTCTGTGTGGGCTCTCACTGGCCGACACCACTCTGGGTCATCGTCGTGTTGTGGGTGGTGGGGGGGGCGCTGTGGGTTCTGGCTGAATGGCTGGAGCg                                                                                                            >  6:98610/1‑134 (MQ=255)
                            ggTTTTGTTGTGTCTTGGGCTTGCAAGTGCAGGCTTCTGTGTGGGCTCTCACTGGCCGACACCACTCTGGGTCATCGTCGTGTTGTGGGTGGTGGGGGGGGCGCTGTGGGTTGTGGGTGAATGGCTGGCGCGgggg                                                                                                        >  4:30880/1‑136 (MQ=255)
                             gTTTTGTTGTGGGTTGGGGTTTCATGTGCTGGGGGCTGGGTGGGCGCTCCCGGGCCGCCACCACTGTGGGGCATGGTGGGGGTGGGGGGGGGGGGGGGGGCGCTGTGGGTTCTGGCTGAATGGCTGGAGCGGAggg                                                                                                       <  4:214676/136‑1 (MQ=255)
                              ttttGTTGTGTCTTGGGCTTGCAAGTGCAGGCTTCTGTGTGGGCTCTCACTGGCCGACACCACTCTGGGTCATCGTCGTGTTGTGGGTGGTGGGGGGGGCGCTGTGGGTTCTGGCTGAATGGCTGGAGCGGAgggg                                                                                                      >  8:28743/1‑136 (MQ=255)
                                ttgttgTGTCTTGGGCTTGCAAGTGCAGGCTTCTGTGTGGGCTCTCACTGGCCGACACCACTCTGGGTCATCGTCGTGTTGTGGGTGGTGGGGGGGGCGCTGTGGGTTCGGGCTGAATGGCTGGAGCGGAGGGGgc                                                                                                    >  8:103199/1‑136 (MQ=255)
                                              gCTTCCACGTGCAGGCTGCGGTGGGGGCTCCCCCGGCCCCACACCACGCTGGGGCTTCGGCGTGTTGGGGGGGGGGGGGGGGGCGCGGGGGGTGCTGGCGGAAGGGCTGGAGCGGAGGGGGCGCAATCAATGATgc                                                                                      <  4:90846/136‑1 (MQ=255)
                                                     aGTGCAGGCTTCTGTGTGGGCTCTCACTGGCCGACACCACTCTGGGTCATCGTCGTGTTGTGGGTGGTGGGGGGGGCGCTGTGGGTTGTGGCTGAATGGGTGGTGCGGAGGGGGGGCAATCAATGATGCGCGccca                                                                               >  7:66495/1‑134 (MQ=255)
                                                     aGTGCAGGCTTCTGTGTGGGCTCTCACTGGCCGACACCACTCTGGGTCATCGTCGTGTTGTGGGTGGTGGGGGGGGCGCTGTGGGTTCTGGCTGAATGGCTGGAGCGGAGGGGGCGCAATCAATGATGCGCGCCaa                                                                               >  7:72881/1‑136 (MQ=255)
                                                     aGTGCAGGCTTCTGTGTGGGCTCTCACTCGCCGACACCACTCTCGGTCATCGTCGTGTTGTGGGTGGTGGGGGGGGCGCGGAGGGTTCTGGCAGAATCGCACGAGCCGAGGGCGCGCAATCAATGAAGCCCCCCa                                                                                >  2:973/1‑135 (MQ=255)
                                                               tCTGTGTGGGCTCTCACTGGCCGACACCACTCTGGGTCATCGTCGTGTTGTGGGTGGTGGGGGGGGCGCTGTGGGTTCTGGCTGAATGGCTGGAGCGGAGGGGGCGCAATCAATGATGCGCGCCAATCTAGACGAg                                                                     >  7:121531/1‑136 (MQ=255)
                                                                      gggCGCGCACTGGCCGACCCCACTGTGGGTCATCGGCGTGTTGTGGGGGGTGGGGGGGGCGCTGTGGGTGCTGGCTGAATGGCTGGAGCGGAGGGGGCGCAATCAATGATGCGCGCCAATCTAGACAAGCTGGTGa                                                              <  7:170967/136‑1 (MQ=255)
                                                                         ctctCACTGGCCGACACCACTCTGGGTCATCGTCGTGTTGTGGGTGGTGGGGGGGGCGCTGTGGGTTCTGGCTGAATGGCTGGAGCGGAGGGGGCGCAATCAATGATGCGCGCCAATCTAGACGAGCTGGTGATCg                                                           >  6:264658/1‑136 (MQ=255)
                                                                         ctctCACTGGCCGACACCACTCTGGGTCATCGTCGTGTTGTGGGGGGTGGGGGGGGCGGGGGGGGTTGGGGCGGGATTGGGGGGGCGGGGGGGGGGGAATCAATGAATCGGGCCCATAAAGAAGTGCTGGTggtag                                                           >  6:54977/1‑132 (MQ=255)
                                                                           ctcACTGGCCGACACCACTCTGGGTCATCGTCGTGTTGTGGGTGGTGGGGGGGGCGCTGTGGGTTCTGGCTGAATGGCTGGAGCGGAGGGGGCGCAATCAATGATGCGCGCCAATCTAGACGAGCTGGTGATCGt                                                          >  8:2787/1‑135 (MQ=255)
                                                                           ctcACTGGCCGACACCACTCTGGGTCATCGTCGTGTTGTGGGTGGTGGGGGGGGCGCGGTGGGTTGTGGCTGAATGGCTGGAGCGGAGGGGGCGCAATCAATGATGCGCGCCAATCTAGACGAGCTGGTGATCGt                                                          >  5:19885/1‑135 (MQ=255)
                                                                           ctcACTGGCCGACACCACTCTGGGTCATCGTCGTGTTGTGGGTGGTGGGGGGGGCGCGGTGGGTTGTGGCTGAATGGCTGGAGCGGAGGGGGCGCAATCAATGATGCGCGCCAATCTAGACGAGCTGGGGATCGTg                                                         >  3:243707/1‑136 (MQ=255)
                                                                           ctcACTGGCCGACACCACTCTGGGTCATCGTCGTGTTGTGGGTGGTGGGGGGGGCGCGGGGGGTTGTGGCGGAATGGCTGGCGCGGAGGGGGGGCAATCAATGATGCGCGCCCATCTAGACGAGCGGGGGATCggt                                                         >  4:90688/1‑134 (MQ=255)
                                                                           ctcACTGGCCGACACCACTCTGGGTCATCGTCGTGTTGTGGGGTGTGGGGGGGGCGCTGGGGGTTGATGCGGAATGGCGGGCGGGGACGGGGCGCAATCAATGATGCGCGCCAATCATGACGCGCTGGTTCTCGTg                                                         >  2:365085/1‑136 (MQ=255)
                                                                           ctcACTGCCCGACACCACTCGGGGTCATGGGCGGGTTGGGGGGGGTGGGGGGGGCGCGGTGGGTTCTGGCTGAATGGCGGGAGCGGAGGGGGCGCAATCAATGATGCGCGCCAATCTAGACGAGCTGGTGATCGTg                                                         <  4:243707/136‑1 (MQ=255)
                                                                                     gccaccccTCTGGGTCATCGGCGGGGGGGGGGGGGGGGGGGGGGCGCTGGGGGTTCTGGCTGAATGGCTGGAGCGGAGGGGGCGCAATCAATGATGCGCGCCAATCTAGACGAGCTGGTGATCGTGAGCtgtttgt                                               <  8:24275/134‑1 (MQ=255)
                                                                                     gccaccacTCGGGGGCATCGGCGTGTGGGGGGGGGGGGGGGGGGCGCGGTGGGTTCTGGCTGAATGGCTGGAGCGGAGGGGGCGCAATCAATGATGCGCGCCAATCTAGACGAGCTGGTGATCGTGAGCtgtttgt                                               <  2:256443/134‑1 (MQ=255)
                                                                                     gACACCACTCTGGGTCATCGTCGTGTTGTGGGTGGTGGGGGGGGCGCTGTGGGTTCTGGCTGAATGGCTGGAGCGGAGGGGGCGCAATCAATGATGCGCGCCAATCTAGACGAGCTGGTGATCGTGAGCtgtttgt                                               >  7:24275/1‑136 (MQ=255)
                                                                                     ccccccacTCTGGGTTATCGGCGGGTGGGGGGTGGGGGGGGGGGCGCTGTGGGTTCTGGCTGAATGGCTGGAGCGGAGGGGGCGCAATCAATGATGCGCGCCAATCTAGACGAGCTGGTGATCGTGAGCtgtttgt                                               <  6:333113/132‑1 (MQ=255)
                                                                                       ccccacTCTGGGGCAGCGTCGGGGTGTGGGTGGGGGGGGGGGCGCTGTGGGTTCTGGCTCAATGGCTGGAGCGGAGGGGGCGCAATCAATGATGCGCGCCAATCTAGACGAGCTGGTGATCGTGAGCtgtttgt                                               <  8:24939/132‑1 (MQ=255)
                                                                                         ccacTCTGGGTCATCGTCGTGTTGTGGGTGGTGGGGGGGGCGCTGTGGGTTCTGGCTGAATGGCTGGAGCGGAGGGGGCGCAATCAATGATGCGCGCCAATCTAGACGAGCTGGTGATCGTGAGCTGTTTGTTTc                                            >  7:9702/1‑135 (MQ=255)
                                                                                          cacTGTGGGGCATCGTCGTGGTGGGGGTGGGGGGGGGGGCGCGGGGGGTTCTGGCTGAATGGCGGGAGCGGAGGGGGCGCAATCAATGATGCGCGCCAATCTAGACGAGCTGGTGATCGTGAGCTGTTTGTTTCCa                                          <  2:355333/136‑1 (MQ=255)
                                                                                                    cATCGTCGTGGTGTGGGTGGTGGGGGGGGCGCTGTGGGTTCTGGCTGAATGGCTGGAGCGGAGGGGGCGCAATCAATGATGCGCGCCAATCTAGACGAGGGGGTGATCGTGAGCTGTTTGTTTCCAGCTATGAggt                                >  3:223643/1‑136 (MQ=255)
                                                                                                     cgcggcgtGGTGGGGGGGGTGGGGGGGGCGCGGGGGGTGCTGGCGGAATGGCTGGCGCGGAGGGGGCGCAATCAATGATGCGCGCCAATCTAGACGAGCTGGTGATCGTGAGCTGTTTGTTTCCAGCTATGAggtg                               <  4:9002/131‑1 (MQ=255)
                                                                                                     aTCGTCGTGTTGTGGGTGGTGGGGGGGGCGCTGTGGGGTGTGGCGGAATGGCTGGAGCGGAGGGGGCGCCATCAATTATGCGCGCCAAACTAGACGAGCTGGTGATCGTGAGCTGTTTGTTTCCAACTATGAggtg                               >  1:301549/1‑136 (MQ=255)
                                                                                                       cggcgtGTGGGGGGGGGTGGGGGGGGCGCTGTGGGGGCTGGCTGAATGGCTGGAGCGGAGGGGGCGCAATCAATGATGCGCGCCAATCTAGACGAGCTGGTGATCGTGAGCTGTTTGTTTCCAGCTATGAggtg                               <  4:3766/131‑1 (MQ=255)
                                                                                                               tgtgGGTGGTGGGGGGGGCGCTGTGGGTTAAGGCTGAATGGCTGGAGCCGAGGGGGCGCAATCAATGATGCGCCCCAAACTAGACGAGCAGGTGATAGTGACCGGTTTGTTTGCAGCAATGAGGTGGTCAGCTTc                      >  4:247187/1‑135 (MQ=255)
                                                                                                                  ggggggggggggggGCGCTGTGGGTTCTGGCTGAATGGCTGGAGCGGAGGGGGCGCAATCAATGATGCGCGCCAATCTAGACGAGCTGGTGATCGTGAGCTGTTTGTTTCCAGCTATGAGGTGGTCAGCTTCTAtt                  <  3:17568/136‑1 (MQ=255)
                                                                                                                      ggtggGGGGGGCGCTGTGGGTTCTGGCTGAATGGCTGGAGCGGAGGGGGCGCAATCAATGATGCGCGCCAATCTAGACGAGCTGGTGATCGTGAGCTGTTTGTTTCCAGCTATGAGGTGGTCAGCTTCTATTgcgc              >  5:272822/1‑136 (MQ=255)
                                                                                                                      ggtggGGGGGGCGCTGTGGGTTCTGGCTGAATGGCTGGAGCGGAGGGGGCGCAATCAATGATGCGCGCCAATCTAGACGAGCTGGTGATCGTGAGCTGTTTGTTTCCAGCTATGAGGTGGTCAGCTTCTATTgcgc              >  4:240060/1‑136 (MQ=255)
                                                                                                                         ggggggggCGCGGGGGGTTCTGGCGGCCTGGCGGGAGCGGAGGGGGCGCAATCAATGATGCGCGCCAATCTAGACGAGCTGGTGATCGTGAGCTGTTTGTTTCCAGCTATGAGGTGGTCAGCTTCTATTGCGCGAt           <  5:63977/136‑1 (MQ=255)
                                                                                                                           ggggggCGCTGTGGGTTCTGGCTGAATGGCTGGAGCGGAGGGGGCGCAATCAATGATGCGCGCCAATCTAGACGAGCTGGTGATCGTGAGCTGTTTGTTTCCAGCTATGAGGTGGTCAGCTTCTATTGCGCGATcc         >  8:344540/1‑136 (MQ=255)
                                                                                                                                  gcTGTGGGTTCTGGCTGAATGGCTGGAGCGGAGGGGGCGCAATCAATGATGCGCGCCAATCTAGACGAGCTGGTGATCGTGAGCTGTTTGTTTCCAGCTATGAGGTGGTCAGCTTCTATTGCGCGATCCGTGCTCa  >  5:326219/1‑136 (MQ=255)
                                                                                                                                    |                                                                                                                                     
CCATGTAGGAGGATCTCCGTATGAGAACGGTTTTGTTGTGTCTTGGGCTTGCAAGTGCAGGCTTCTGTGTGGGCTCTCACTGGCCGACACCACTCTGGGTCATCGTCGTGTTGTGGGTGGTGGGGGGGGCGCTGTGGGTTCTGGCTGAATGGCTGGAGCGGAGGGGGCGCAATCAATGATGCGCGCCAATCTAGACGAGCTGGTGATCGTGAGCTGTTTGTTTCCAGCTATGAGGTGGTCAGCTTCTATTGCGCGATCCGTGCTCA  >  ACB143/740542‑740807

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑