Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB143	1,550,590	C→T	5.7%	K213K (AAG→AAA)	CJ019_01464 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB143	1,550,590	0	C	T	5.7%	244.6 / 3.2	70	K213K (AAG→AAA)	CJ019_01464	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (44/22);  new base T (2/2);  total (46/24)
Fisher's exact test for biased strand distribution p-value = 6.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.00e-01

GCGACAGCATCTGCACCAGGAAGCTGGGAATCCAGTGCAGGTCTACCCCCAAGCCCCGCAGCACCAGGTACAAGACGCTGTAGCGCAGTCCCCAGTGGATGCAGGTCAGGGTGAACACCAGGCTCAGCCTGCGCTTGGGCAGGCGCCAGGTCTGCGCCAGTGCTGCAACGAAGTGCAGCAGTTTGCGCGCCCAGCGACGTTTGCGCGGCTGGCCCATCCCCATGCGCTGCAACAGCCTGCCGTTCATGCGCATTATCGGGCGCCG  >  ACB143/1550457‑1550721                                                                                                                                      |                                                                                                                                    gCGACAGCATCTGCACCAGGAAGCTGGGAATCCAGTGCAGGTCTACCCCCAAGCCCCGCAGCACCAGGTACAAGACGCTGTAGCGCAGTCCCCAGTGGATGCAGGTCAGGGTGAACACCAGGCTCAGCCTGCGCtt                                                                                                                                   <  4:187736/136‑1 (MQ=255) gCGACAGCATCTGCACCAGGAAGCTGGGAATCCAGTGCAGGTCTACCCCCAAGCCCCGCAGCACCAGGTACAAGACGCTGTAGCGCAGTCCCCAGTGGATGCAGGTCAGGGTGAACACCAGGCTCAGCCTGCGCtt                                                                                                                                   <  4:232184/136‑1 (MQ=255) gCGACAGCATCTGCACCAGGAAGCTGGGAATCCAGTGCAGGTCTACCCCCAAGCCCCGCAGCACCAGGTACAAGACGCTGTAGCGCAGTCCCCAGTGGATGCAGGTCAGGGTGAACACCAGGCTCAGCCTGCGCtt                                                                                                                                   <  7:279522/136‑1 (MQ=255)   gACAGCATCTGCACCAGGAAGCTGGGAATCCAGTGCAGGTCTACCCCCAAGCCCCGCAGCACCAGGTACAAGACGCTGTAGCGCAGTCCCCAGTGGATGCAGGTCAGGGTGAACACCAGGCTCAGCCTGCGCTTgg                                                                                                                                 <  3:111720/136‑1 (MQ=255)   gACAGCATCTGCACCAGGAAGCTGGGAATCCAGTGCAGGTCTACCCCCAAGCCCCGCAGCACCAGGTACAAGACGCTGTAGCGCAGTCCCCAGTGGATGCAGGTCAGGGTGAACACCAGGCTCAGCCTGCGCTTgg                                                                                                                                 <  6:311557/136‑1 (MQ=255)   gACAGCATCTGCACCAGGAAGCTGGGAATCCAGTGCAGGTCTACCCCCAAGCCCCGCAGCACCAGGTACAAGACGCTGTAGCGCAGTCCCCAGTGGATGCAGGTCAGGGTGAACACCAGGCTCAGCCTGCGCTTgg                                                                                                                                 <  2:105544/136‑1 (MQ=255)   gACAGCATCTGCACCAGGAAGCTGGGAATCCAGTGCAGGTCTACCCCCAAGCCCCGCAGCACCAGGTACAAGACGCTGTAGCGCAGTCCCCAGTGGATGCAGGTCAGGGTGAACACCAGGCTCAGCCTGCGCTTgg                                                                                                                                 <  7:109225/136‑1 (MQ=255)             gCACCAGGAAGCTGGGAATCCAGTGCAGGTCTACCCCCAAGCCCCGCAGCACCAGGTACAAGACGCTGTAGCGCAGTCCCCAGTGGATGCAGGTCAGGGTGAACACCAGGCTCAGCCTGCGCTTGGGCAGgcgc                                                                                                                         >  7:359302/1‑134 (MQ=255)                   ggAAGCTGGGAATCCAGTGCAGGTCTACCCCCAAGCCCCGCAGCACCAGGTACAAGACGCTGTAGCGCAGTCCCCAGTGGATGCAGGGCAGGGTGAACACCAGGCTCAGCCTGCGCTTGGGCAGGCGCCAGGTCTg                                                                                                                 <  2:315994/136‑1 (MQ=255)                      aGCTGGGAATCCAGTGCAGGTCTACCCCCAAGCCCCGCAGCACCAGGTACAAGACGCTGTAGCGCAGTCCCCAGGGGATGCAGGTCAGGGTGAACACCAGGCTCAGCCTGCGCTTGGGCAGGCGCCAGGTCTgcgc                                                                                                              <  6:380476/136‑1 (MQ=255)                        cTGGGAATCCAGTGCAGGTCTACCCCCAAGCCCCGCAGCACCAGGTACAAGACGCTGTAGCGCAGTCCCCAGTGGATGCAGGTCAGGGTGAACACCAGGCTCAGCCTGCGCTTGGGCAGGCGCCAGGTCTGCGCCa                                                                                                            <  5:275244/136‑1 (MQ=255)                          gggAATCCAGTGCAGGTCTAAAAACAAGCCCCGCAGCACCAGGTACAAGACGCTGTAGCGCAGTCCCCAGTGGATGCAGGTCAGGGTGAACACCAGGCTCAGCCTGCGCTTGGGCAGGCGCCAGGTCTGCGCCAGt                                                                                                          >  5:317858/1‑136 (MQ=255)                           ggAATCCAGTGCAGGTCTACCCCCAAGCCCCGCAGCACCAGGTACAAGACGCTGTAGCGCAGTCCCCAGTGGATGCAGGTCAGGGTGAACACCAGGCTCAGCCTGCGCTTGGGCAGGCGCCAGGTCTGCGCCAGt                                                                                                          >  4:56648/1‑135 (MQ=255)                           ggAATCCAGTGCAGGTCTACCCCCAAGCCCCGCAGCACCAGGTACAAGACGCTGTAGCGCAGTCCCCAGTGGATGCAGGTCAGGGTGAACACCAGGCTCAGCCTGCGCTTGGGCAGGCGCCAGGTCTGCGCCAGt                                                                                                          <  3:56648/135‑1 (MQ=255)                                 cAGTGCAGGTCTACCCCCAAGCCCCGCAGCACCAGGTACAAGACGCTGTAGCGCAGTCCCCAGTGGATGCAGGTCAGGGTGAACACCAGGCTCAGCCTGCGCTTGGGCAGGCGCCAGGTCTGCGCCAGTGCTGCaa                                                                                                   >  3:168248/1‑136 (MQ=255)                                 cAGTGCAGGTCTACCCCCAAGCCCCGCAGCACCAGGTACAAGACGCTGTAGCGCAGTCCCCAGTGGATGCAGGTCAGGGTGAACACCAGGCTCAGCCTGCGCTTGGGCAGGCGCCAGGTCTGCGCCAGTGCTGCaa                                                                                                   <  3:242104/136‑1 (MQ=255)                                     gCAGGTCTACCCCCAAGCCCCGCAGCACCAGGTACAAGACGCTGTAGCGCAGTCCCCAGTGGATGCAGGTCAGGGTGAACACCAGGCTCAGCCTGCGCTTGGGCAGGCGCCAGGTCTGCGCCAGTGCTGCAACg                                                                                                 >  2:127137/1‑134 (MQ=255)                                            tACCCCCAAGCCCCGCAGCACCAGGTACAAGACGCTGTAGCGCAGTCCCCAGTGGATGCAGGTCAGGGTGAACACCAGGCTCAGCCTGCGCTTGGGCAGGCGCCAGGTCTGCGCCAGTGCTGCAACGAAGTgcag                                                                                         >  1:86510/1‑135 (MQ=255)                                            tACCCCCAAGCCCCGCAGCACCAGGTACAAGACGCTGTAGCGCAGTCCCCAGTGGATGCAGGTCAGGGTGAACACCAGGCTCAGCCTGCGCTTGGGCAGGCGCCAGGTCTGCGCCAGTGCTGCAACGAAGTgcag                                                                                         >  5:281878/1‑135 (MQ=255)                                                 ccAAGCCCCGCAGCACCAGGTACAAGACGCTGTAGCGCAGTCCCCAGTGGATGCAGGTCAGGGTGAACACCAGGCTCAGCCTGCGCTTGGGCAGGCGCCAGGTCTGCGCCAGTGCTGCAACGAAGTGCAGCAGttt                                                                                   >  6:133230/1‑136 (MQ=255)                                                    aGCCCCGCAGCACCAGGTACAAGACGCTGTAGCGCAGTCCCCAGTGGATGCAGGTCAGGGTGAACACCAGGCTCAGCCTGCGCTTGGGCAGGCGCCAGGTCTGCGCCAGTGCTGCAACGAAGTGCAGCAGTTTgcg                                                                                <  3:153923/136‑1 (MQ=255)                                                      ccccGCAGCACCAGGTACAAGACGCTGTAGCGCAGTCCCCAGTGGATGCAGGTCAGGGTGAACACCAGGCTCAGCCTGCGCTTGGGCAGGCGCCAGGTCTGCGCCAGTGCTGCAACGAAGTGCAGCAGTTTgcgcg                                                                              >  5:379420/1‑136 (MQ=255)                                                      ccccGCAGCACCAGGTACAAGACGCTGTAGCGCAGTCCCCAGTGGATGCAGGTCAGGGTGAACACCAGGCTCAGCCTGCGCTTGGGCAGGCGCCAGGTCTGCGCCAGTGCTGCAACGAAGTGCAGCAGTTTgcgcg                                                                              >  5:336909/1‑136 (MQ=255)                                                            agcaCCAGGTACAAGACGCTGTAGCGCAGTCCCCAGTGGATGCAGGTCAGGGTGAACACCAGGCTCAGCCTGCGCTTGGGCAGGCGCCAGGTCTGCGCCAGTGCTGCAACGAAGTGCAGCAGTTTGCGCGCCCAGc                                                                        >  4:256764/1‑136 (MQ=255)                                                                      aCAAGACGCTGTAGCGCAGTCCCCAGTGGATGCAGGTCAGGGTGAACACCAGGCTCAGCCTGCGCTTGGGCAGGCGCCAGGTCTGCGCCAGTGCTGCAACGAAGTGCAGCAGTTTGCGCGCCCAGCGACGTTTgcg                                                              >  4:66002/1‑136 (MQ=255)                                                                      aCAAGACGCTGTAGCGCAGTCCCCAGTGGATGCAGGTCAGGGTGAACACCAGGCTCAGCCTGCGCTTGGGCAGGCGCCAGGTCTGCGCCAGTGCTGCAACGAAGTGCAGCAGTTTGCGCGCCCAGCGACGTTTgcg                                                              >  8:354901/1‑136 (MQ=255)                                                                      aCAAGACGCTGTAGCGCAGTCCCCAGTCGATGCAGGACAGGGTGAACACCAGGCTCACCCTGCGCTTGGGCATGCGCCAGGTCTCCGCCAGTGCTGCAACGAAATGCAGCAGTTTCCGCGCCCAGCGAAGTTTgcg                                                              >  5:202035/1‑136 (MQ=255)                                                                       cAAGACGCTGTAGCGCAGTCCCCAGTGGATGCAGGTCAGGGTGAACACCAGGCTCAGCCTGCGCTTGGGCAGGCGCCAGGTCTGCGCCAGTGCTGCAACGAAGTGCAGCAGTTTGCGCGCCCAGCGACGTTTgcgc                                                             <  6:207864/136‑1 (MQ=255)                                                                           aCGCTGTAGCGCAGTCCCCAGTGGATGCAGGTCAGGGTGAACACCAGGCTCAGCCTGCGCTTGGGCAGGCGCCAGGTCTGCGCCAGTGCTGCAACGAAGTGCAGCAGTTTGCGCGCCCAGCGACGTTTGCGCGGCt                                                         >  6:262656/1‑136 (MQ=255)                                                                              cTGTAGCGCAGTCCCCAGTGGATGCAGGTCAGGGTGAACACCAGGCTCAGCCTGCGCTTGGGCAGGCGCCAGGTCTGCGCCAGTGCTGCAACGAAGTGCAGCAGTTTGCGCGCCCAGCGACGTTTGCGCGGCTGGc                                                      <  4:167243/136‑1 (MQ=255)                                                                                gTAGCGCAGTCCCCAGTGGATGCAGGTCAGGGTGAACACCAGGCTCAGCCTGCGCTTGGGCAGGCGCCAGGTCTGCGCCAGTGCTGCAACGAAGTGCAGCAGTTTGCGCGCCCAGCGACGTTTGCGCGGCTGGcc                                                     >  8:343998/1‑135 (MQ=255)                                                                                  aGCGCAGTCCCCAGTGGATGCAGGTCAGGGTGAACACCAGGCTCAGCCTGCGCTTGGGCAGGCGCCAGGTCTGCGCCAGTGCTGCAACGAAGTGCAGCAGTTTGCGCGCCCAGCGACGTTTGCGCGGCTGGCCCAt                                                  >  6:157159/1‑136 (MQ=255)                                                                                  aGCGCAGTCCCCAGTGGATGCAGGTCAGGGTGAACACCAGGCTCAGCCTGCGCTTGGGCAGGCGCCAGGTCTGCGCCAGTGCTGCAACGAAGTGCAGCAGTTTGCGCGCCCAGCGACGTTTGCGCGGCTGGCCCAt                                                  >  3:276017/1‑136 (MQ=255)                                                                                  aGCGCAGTCCCCAGTGGATGCAGGTCAGGGTGAACACCAGGCTCAGCCTGCGCTTGGGCAGGCGCCAGGTCTGCGCCAGTGCTGCAACGAAGTGCAGCAGTTTGCGCGCCCAGCGACGTTTGCGCGGCTGGCCCAt                                                  >  3:241631/1‑136 (MQ=255)                                                                                  aGCGCAGTCCCCAGTGGATGCAGGTCAGGGTGAACACCAGGCTCAGCCTGCGCTTGGGCAGGCGCCAGGTCTGCGCCAGTGCTGCAACGAAGTGCAGCAGTTTGCGCGCCCAGCGACGTTTGCGCGGCTGGCCCAt                                                  >  6:372355/1‑136 (MQ=255)                                                                                  aGCGCAGTCCCCAGTGGATGCAGGTCAGGGTGAACACCAGGCTCAGCCTGCGCTTGGGCAGGCGCCAGGTCTGCGCCAGTGCTGCAACGAAGTGCAGCAGTTTGCGCGCCCAGCGACGTTTGCGCGGCTGGCCCAt                                                  >  7:224275/1‑136 (MQ=255)                                                                                  aGCGCAGTCCCCAGTGGATGCAGGTCAGGGTGAACACCAGGCTCAGCCTGCGCTTGGGCAGGCGCCAGGTCTGCGCCAGTGCTGCAACGAAGTGCAGCAGTTTGCGCGCCCAGCGACGTTTGCGCGGCTGGCCCAt                                                  >  8:300684/1‑136 (MQ=255)                                                                                    cgcCGTCCCCAGTGGCTGCAGGGCAGGGTGAACACGAGGCTCAGCCTGCGCTTGGGCAGGCGCCAGGTCTGCGCCAGTGCTGCAACGAAGTGCAGGATTTTGCGCGCCCAGCGACGTTTGAGCGGCTGGCCCATcc                                                <  5:1881/136‑1 (MQ=255)                                                                                      cAGTCCCCAGTGGATGCAGGTCAGGGTGAACACCAGGCTCAGCCTGCGCTTGGGCAGGCGCCAGGTCTGCGCCAGTGCTGCAACGAAGTGCAGCAGTTTGCGCGCCCAGCGACGTTTGCGCGGCTGGCCCATcccc                                              <  4:168248/136‑1 (MQ=255)                                                                                       aGTCCCCAGTGGATGCAGGTCAGGGTGAACACCAGGCTCAGCCTGAGCTTGGGCAGGCGCCAGGTCTGCGCCAGTGCAGCAACGAAGTGCAGCAGTTTGCGCGCCCAGCGACGTTTGCGAGGCTGGCCCATcccc                                              >  6:219030/1‑135 (MQ=255)                                                                                        gtgcccAGTGGATTCAGGTCAGGGTGAACACCAGGATCAGCCTTCGCTTGGGCAGGCGCCAGGTCTTCGCCAGTGCTGCAACGAAGTGCAGCAGTTTGCGCGCCCAGCGACGTTTGCGCGGCTGGCCCATCCCCAt                                            <  8:71288/133‑1 (MQ=255)                                                                                           cccAGTGGATGCAGTTCAGGGTGAACACCAGGCTCAGCCTGCGCTTGGGCAGGCGCCAGGTCTGCGCCAGTGCTGCAACGAAGTGCAGCAGTTTGCGCGCCCAGCGACGTTTGCGCGGCTGGCCCATCCCCATgcg                                         <  7:314648/136‑1 (MQ=255)                                                                                            ccAGTGGATGCAGGTCAGGGTGAACACCAGGCTCAGCCTGCGTTTGGGCAGGCGCCAGGTCTGCGCCAGTGCTGCa                                                                                                    >  1:14967/1‑76 (MQ=255)                                                                                            ccAGTGGATGCAGGTCAGGGTGAACACCAGGCTCAGCCTGCGTTTGGGCAGGCGCCAGGTCTGCGCCAGTGCTGCa                                                                                                    >  1:8390/1‑76 (MQ=255)                                                                                            ccAGTGGATGCAGGTCAGGGTGAACACCAGGCTCAGCCTGCGTTTGGGCAGGCGCCAGGTCTGCGCCAGTGCTGCa                                                                                                    <  2:14967/76‑1 (MQ=255)                                                                                            ccAGTGGATGCAGGTCAGGGTGAACACCAGGCTCAGCCTGCGTTTGGGCAGGCGCCAGGTCTGCGCCAGTGCTGCa                                                                                                    <  2:8390/76‑1 (MQ=255)                                                                                            ccAGTGGATGCAGGTCAGGGTGAACACCAGGCTCAGCCTGCGCTTGGGCAGGCGCCAGGTCTGCGCCAGTGCTGCAACGAAGTGCAGCAGTTTGCGCGCCCAGCGACGTTTGCGCGGCTGGCCCATCCCCATgcgc                                        >  5:171698/1‑136 (MQ=255)                                                                                            ccAGTGGATGCAGGTCAGGGTGAACACCAGGCTCAGCCTGCGCTTGGGCAGGCGCCAGGTCTGCGCCAGTGCTGCAACGAAGTGCAGCAGTTTGCGCGCCCAGCGACGTTTGCGCGGCTGGCCCATCCCCATgcgc                                        >  2:77446/1‑136 (MQ=255)                                                                                            caaGTGGATGCAGGTAAGGGTGAACACCAGGATCAGCCTGCGCTTGGGCAGGCGCCAGGTCTGCGCCAGTGCTGCAACGAAGTGCAGCAGTTTGCGCGCCCAGCGACGTTAGCGCGGCTGGCCCATCCCCATgcgc                                        >  3:340228/3‑136 (MQ=255)                                                                                              aGTGGATGCAGGTCAGGGTGAACACCAGGCTCAGCCTGCGCTTGGGCAGGCGCCAGGTCTGCGCCAGTGCTGCAACGAAGTGCAGCAGTTTGCGCGCCCAGCGACGTTTGCGCGGCTGGCCCATCCCCATGCGCTg                                      >  4:193381/1‑136 (MQ=255)                                                                                              aGTGGATGCAGGTCAGGGTGAACACCAGGCTCAGCCTGCGCTTGGGCAGGCGCCAGGTCTGCGCCAGTGCTGCAACGAAGTGCAGCAGTTTGCGCGCCCAGCGACGTTTGCGCGGCTGGCCCATCCCCATGCGCTg                                      >  5:281469/1‑136 (MQ=255)                                                                                                      cAGGTCAGGGTGAACACCAGGCTCAGCCTGCGCTTGGGCAGGCGCCAGGTCTGCGCCAGTGCTGCAACGAAGTGCAGCAGTTTGCGCGCCCAGCGACGTTTGCGCGGCTGGCCCATCCCCATGCGCTGCAACAGcc                              <  6:317858/136‑1 (MQ=255)                                                                                                      cAGGTCAGGGTGAACACCAGGCTCAGCCTGCGCTTGGGCAGGCGCCAGGTCTGCGCCAGTGCTGCAACGAAGTGCAGCAGTTTGCGCGCCCAGCGACGTTTGCGCGGCTGGCCCATCCCCATGCGCTGCAACAGcc                              >  1:399386/1‑136 (MQ=255)                                                                                                      cAGGTCAGGGTGAACACCAGGCTCAGCCTGCGCTTGGGCAGGCGCCAGGTCTGCGCCAGTGCTGCAACGAAGTGCAGCAGTTTGCGCGCCCAGCGACGTTTGCGCGGCTGGCCCATCCCCATGCGCTGCAACAGcc                              >  8:272889/1‑136 (MQ=255)                                                                                                      cAGGTCAGGGTGAACACCAGGCTCAGCCTGCGCTTGGGCAGGCGCCAGGTCTGCGCCAGTGCTGCAACGAAGTGCAGCAGTTTGCGCGCCCAGCGACGTTTGCGCGGCTGGCCCATCCCCATGCGCTGCAACAGcc                              >  7:209611/1‑136 (MQ=255)                                                                                                      cAGGTCAGGGTGAACACCAGGCTCAGCCTGCGCTTGGGCAGGCGCCAGGTCTGCGCCAGTGCTGCAACGAAGTGCAGCAGTTTGCGCGCCCAGCGACGTTTGCGCGGCTGGCCCATCCCCATGCGCTGCAACAGcc                              >  3:163803/1‑136 (MQ=255)                                                                                                      cAGGTCAGGGTGAACACCAGGCTCAGCCTGCGCTTGGGCAGGCGCCAGGTCTGCGCCAGTGCTGCAACGAAGTGCAGCAGTTTGCGCGCCCAGCGACGTTTGCGCGGCTGGCCCATCCCCATGCGCTGCAACAGcc                              >  5:7176/1‑136 (MQ=255)                                                                                                        ggTCAGGGTGAACACCAGGCTCAGCCTGCGCTTGGGCAGGCGCCAGGTCTGCGCCAGTGCTGCAACGAAGTGCAGCAGTTTGCGCGCCCAGCGACGTTTGCGCGGCTGGCCCATCCCCATGCGCTGCAACAGCCTg                            >  6:312855/1‑136 (MQ=255)                                                                                                        ggTCAGGGTGAACACCAGGCTCAGCCTGCGCTTGGGCAGGCGCCAGGTCTGCGCCAGTGCTGCAACGAAGTGCAGCAGTTTGCGCGCCCAGCGACGTTTGCGCGGCTGGCCCATCCCCATGCGCTGCAACAGCCTg                            >  5:56695/1‑136 (MQ=255)                                                                                                           cAGGGTGAACACCAGGCTCAGCCTGCGCTTGGGCAGGCGCCAGGTCTGCGCCAGTGCTGCAACGAAGTGCAGCAGTTTGCGCGCCCAGCGACGTTTGCGCGGCTGGCCCATCCCCATGCGCTGCAACAGCCTGCCg                         >  5:40661/1‑136 (MQ=255)                                                                                                             gggTGAACACCAGGCTCAGCCTGCGCTTGGGCAGGCGCCAGGTCTGCGCCAGTGCTGCAACGAAGTGCAGCAGTTTGCGCGCCCAGCGACGTTTGCGCGGCTGGCCCATCCCCATGCGCTGCAACAGCCTGCCGtt                       <  2:164245/136‑1 (MQ=255)                                                                                                              ggTGAACACCAGGCTCAGCCTGCGCTTGGGCAGGCGCCAGGTCTGCGCCAGTGCTGCAACGAAGTGCAGCAGTTTGCGCGCCCAGCGACGTTTGCGCGGCTGGCCCATCCCCATGCGCTGCAACAGCCTGCCGTTc                      <  6:40661/136‑1 (MQ=255)                                                                                                                      ccAGGCTCAGCCTGCGCTTGGGCAGGCGCCAGGTCTGCGCCAGTGCTGCAACGAAGTGCAGCAGTTTGCGCGCCCAGCGACGTTTGCGCGGCTGGCCCATCCCCATGCGCTGCAACAGCCTGCCGTTCATGCGCAt              >  2:306792/1‑136 (MQ=255)                                                                                                                      ccAGGCTCAGCCTGCGCTTGGGCAGGCGCCAGGTCTGCGCCAGTGCTGCAACGAAGTGCAGCAGTTTGCGCGCCCAGCGACGTTTGCGCGGCTGGCCCATCCCCATGCGCTGCAACAGCCTGCCGTTCATGCGCAt              >  2:180711/1‑136 (MQ=255)                                                                                                                      ccAGGCTCAGCCTGCGCTTGGGCAGGCGCCAGGTCTGCGCCAGTGCTGCAACGAAGTGCAGCAGTTTGCGCGCCCAGCGACGTTTGCGCGGCTGGCCCATCCCCATGCGCTGCAACAGCCTGCCGTTCATGCGCAt              >  4:153496/1‑136 (MQ=255)                                                                                                                      ccAGGCTCAGCCTGCGCTTGGGCAGGCGCCAGGTCTGCGCCAGTGCTGCAACGAAGTGCAGCAGTTTGCGCGCCCAGCGACGTTTGCGCGGCTGGCCCATCCCCATGCGCTGCAACAGCCTGCCGTTCATGCGCAt              >  1:48936/1‑136 (MQ=255)                                                                                                                           cTCAGCCTGCGCTTGGGCAGGCGCCAGGTCTGCGCCAGTGCTGCAACGAAGTGCAGCAGTTTGCGCGCCCAGCGACGTTTGCGCGGCTGGCCCATCCCCATGCGCTGCAACAGCCTGCCGTTCATGCGCATTATCg         >  5:44287/1‑136 (MQ=255)                                                                                                                           cTCAGCCTGCGCTTGGGCAGGCGCCAGGTCTGCGCCAGTGCTGCAACGAAGTGCAGCAGTTTGCGCGCCCAGCGACGTTTGCGCGGCTGGCCCATCCCCATGCGCTGCAACAGCCTGCCGTTCATGCGCATTATCg         >  1:273073/1‑136 (MQ=255)                                                                                                                           cTCAGCCTGCGCTTGGGCAGGCGCCAGGTCTGCGCAAGTGCTGCAACGAAGTGCAGCAGTTTGCGCGCCCACCGACGTTTGCGCGGCTGGCCCATCCCCATGCGCTGCAACAGCCTGCCGTTCATGCGCATTATCg         >  6:287683/1‑136 (MQ=255)                                                                                                                                 cTGCGCTTGGGCAGGCGCCAGGTCTGCGCCAGTGCTGCAACGAAGTGCAGCAGTTTGCGCGCCCAGCGACGTTTGCGCGGCTGGCCCATCCCCATGCGCTGCAACAGCCTGCCGTTCATGCGCATTATCGGGCGcc   >  3:170387/1‑136 (MQ=255)                                                                                                                                    cgcTTGGGCAGGCGCCAGGTCTGCGCCAGTGCTGCAACGAAGTGCAGCAGGTTGCGCGCCCAGCGACGTTTGCGCGGCTGGCCCATCCCCATGCGCTGCAACAGCCTGCCGTTCATGCGCATTATCGGGCGCCg  <  5:372509/134‑1 (MQ=255)                                                                                                                                      |                                                                                                                                    GCGACAGCATCTGCACCAGGAAGCTGGGAATCCAGTGCAGGTCTACCCCCAAGCCCCGCAGCACCAGGTACAAGACGCTGTAGCGCAGTCCCCAGTGGATGCAGGTCAGGGTGAACACCAGGCTCAGCCTGCGCTTGGGCAGGCGCCAGGTCTGCGCCAGTGCTGCAACGAAGTGCAGCAGTTTGCGCGCCCAGCGACGTTTGCGCGGCTGGCCCATCCCCATGCGCTGCAACAGCCTGCCGTTCATGCGCATTATCGGGCGCCG  >  ACB143/1550457‑1550721

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑