Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 1,630,560 T→A 19.1% V412V (GTA→GTT)  czcS ← Sensor protein CzcS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1431,630,5600TA19.1% 65.7 / 3.3 40V412V (GTA→GTT) czcSSensor protein CzcS
Reads supporting (aligned to +/- strand):  ref base T (18/14);  new base A (3/5);  total (21/19)
Fisher's exact test for biased strand distribution p-value = 4.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTGCATGATCGACTTCACGATCGCCAACCCCAACCCGGTCCCGCCCTCCAGCCGCGAACGCCCCGAACCTACCCGGTAAAACCGCTCGAACAAGCGCGACTGGTGCTCTGCAGGCACACCCGGACCCTGGTTCTCCACGGATAGAATGACTTCGCTGCCCGAGCGTTCGATGCGCAGAGCCACGGGTTTACCCTCGGG  >  ACB143/1630425‑1630688
                                                                                                                                       |                                                                                                                                
aaaCACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTGCATGATCGACTTCACGATCGCCAACCCCAACCCGGTCCCGCCCTCCAGCCGCGAACGCCCCGAACCt                                                                                                                                  <  7:265582/136‑1 (MQ=255)
       aGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTGCATGATCGACTTCACGATCGCCAACCCCCACCCCGTCCCGCCCTCCACCCGCGCAAGCCCCGAACCTAccccg                                                                                                                            >  2:257095/1‑133 (MQ=255)
            aaaCCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTGCATGATCGACTTCACGATCGCCAACCCCCACCCGGTCCCGCCCTCCAGCCGCGGACGCCCCGAACCTACCCGGTAAAAc                                                                                                                      >  7:303228/1‑136 (MQ=255)
                 gggTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCACGCCATGCAACTGCATGATCGACTTCACGATCGCCAACCCCAACCCGGTCCCGCCCTACACCCCCGAACGCCCCCAACCAACCCGGTAAAACCGcgc                                                                                                                 >  7:263798/1‑134 (MQ=255)
                      ggCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTGCATGATCGACTTCACGATCGCCAACCCCAACCCGGTCCCGCCCTCCAGCCGCGAACGCCCCGCACCTACCCCGTAAAACCGCGCGAAc                                                                                                             >  4:318285/1‑135 (MQ=255)
                        ccccAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTCCATGATCGACTTCACGATCGCCAACCCCAACCCGGTCCCGCCCTCCAGCCGCGAACGCCCCGAACCTACCCGGTAAAACCGCTCGAACAAg                                                                                                          <  1:242948/136‑1 (MQ=255)
                           cAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATCCAACTGCATGATCGACTTCACGATCGCCAACCCCAACCCGGTCCCGCCCTCCAGCCGCGAACGCCCCGAACCTACCCGATAAAACCGCTCGAACACgcgc                                                                                                       <  3:22047/136‑1 (MQ=255)
                                ggCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTGCATGATCGACTTCACGATCGCCAACCCCAACCCGGTCCCGCCCTCCAGCCGCGCACGCCCCGAACCTACCCGGTAAAACCGCTCGAACAAGCGCGACTg                                                                                                  >  3:49294/1‑136 (MQ=255)
                                    gctgctGACCTCGACCCGCCCGCCATGCAACTGCATGATCGACTTCACGATCGCCAACCCCAACCCGGTCCCGCCCTACAGCCGCGAACGCCCCGAACCTACCCGGTAAAACCGCTCGAACAAGCGCGACTGGTGc                                                                                              >  7:318402/1‑136 (MQ=255)
                                     ctgctgACCTCGACCCGCCCGCCATGCAACTGCATGATCGACTTCACGATCGCCAACCCCAACCCGGTCCCGCCCACCACCCGCGAACGCCCCGAACCTACCCGGTAAAACCCCTCGAACAAGCGCGACTGGTGct                                                                                             >  3:122978/1‑136 (MQ=255)
                                      tgctgACCTCGACCCGCCCGCCATGCAACTGCATGATCGACTTCACGATCGCCAACCCCAACCCGGTCCCGCCCTCCAGCCGCGAACGCCCCGAACCTACCCGGTAAAACCGCTCGAACAAGCGCGACTGGTGctc                                                                                            >  4:151640/1‑136 (MQ=255)
                                        ctgACCTCGACCCGCCCGCCATGCAACTGCATGATCGACTTCACGATCGCCAACCCCAACCCGGTCCCGCCCTCCCGCCGCGAACGCCCCGAACCTACCCGGTAAAACCGCGCGAACAAGCGCGACTGGTGCTCTg                                                                                          >  5:64748/1‑136 (MQ=255)
                                               cgccccgcccgCCATGCAACTGCATGATCGACTTCACGATCGCCAACCCCAACCCGGTCCCGCCCTCCAGCCGCGAACGCCCCGAACCTACCCGGTAAAACCGCTCGAACAAGCGCGACTGGTGCTCTGCAGGcac                                                                                   <  5:380588/133‑1 (MQ=255)
                                                gACCCGCCCGCCATGCAACTGCATGATCGACTTCACGATCGCCAACCCCAACCCCGTCCCGCCCACCACCCGCGCACGCCCCGAACCAACCCGGTAAAACCGCACGAACAACCGCGACGGGTGCTCGGCAGGcaca                                                                                  >  8:170930/1‑136 (MQ=255)
                                                   ccgcccgcCATGCAACTGCATGATCGACTTCACGATCGCCAACCCCAACCCGGTCCCGCCCTCCAGCCGCGAACGCCCCGAACCTACCCGGTAAAACCGCTCGAACAAGCGCGACTGGTGCTCTGCAGGCACAccc                                                                               >  6:172710/1‑136 (MQ=255)
                                                     gcccgccATGCAACTGCATGATCGACTTCACGATCGCCAACCCCAACCCGGTCCCGCCCTCCAGCCGCGCACGCCCCGAACCTACCCGGTAAAACCGCTCGAACAAGCGCGACTGGTGCTCTGCAGGCACACCCgg                                                                             >  2:261547/1‑136 (MQ=255)
                                                          ccATGCAACTGCATGATCGACTTCACGATCGCCAACCCCAACCCGGTCCCGCCCTCCAGCCGCGAACGCCCCGAACCTACCCGGTAAAACCGCTCGAACAAGCGCGACTGGTGCTCTGCAGGCACACCCGGACCCt                                                                        <  1:272403/136‑1 (MQ=255)
                                                                     cATGATCGACTTCACGATCGCCAACCCCAACCCGGTCCCGCCCTCCAGCCGCGAACGCCCCGAACCTACCCGTTAAAACCGCTCGAACAAGCGCGACTGGTGCTCTGCAGGCACACCCGGACCCTGGTTCTCCAc                                                              <  7:374702/135‑1 (MQ=255)
                                                                          tCGACTTCACGATCGCCAACCCCAACCCGGTCCCGCCCTCCAGCCGCGAACGCCCCGAACCTACCCGGTAAAACCGCTCGAACAAGCGCGACTGGTGCTCTGCAGGCACACCCGGACCCTGGTTCTCCACGGa                                                           >  7:42615/1‑133 (MQ=255)
                                                                          tCGACTTCACGATCGCCAACCCCAAACCGGTCCCGCCCACCAGCCGCGCACGCCCCCCACCAACCCCGGAAAACCGCTCCAACCATCGCCACTGGTGCACAGCAGGCAAACCCCGACCCTGGTTCTCCCCggcaa                                                         >  7:368600/1‑132 (MQ=255)
                                                                           cGACTACACCATCGCAAACCCCACCCCGGGCCCCCCCTCCAGCCGCGAACGCCCCCAACCAACCCGTAAAAACCGCGCGACCACCCGCGACTGGTGCTCTGCAGGCACACCCGGACCCTGGTTCTCCACGGATAGa                                                       <  1:166616/136‑1 (MQ=255)
                                                                                    gATCGCCAACCCCAACCCGGTCCCGCCCTCCAGCCGCGAACGCCCCGAACCTACCCGGTAAAACCGCTCGAACAAGCGCGACTGGTGCTCTGCAGGCACACCCGGACCCTGGTTCTCCACGGATAGAATGACTTCg                                              >  1:64911/1‑136 (MQ=255)
                                                                                      tCGCCAACCCCAACCCGGTCCCGCCCTCCAGCCGCGCACGCCCCGAACCTACCCGGTAAAACCGCTCGAACAAGCGCGACTGGTGCTCTGCAGGCACACCCGGACCCTGGGTCTCCACGGATAGAATGAATTCGCt                                            >  6:58063/1‑136 (MQ=255)
                                                                                                      ggTCCCGCCCTCCAGCCGCGAACGCCCCGAACCTACCCGGTAAAACCGCTCGAACAAGCGCGACTGGTGCTCTGCAGGCACACCCGGACCCTGGTTCTCCACGGATAGAATGACTTCGCTGCCCGAGCGTTCGATg                            <  8:34910/136‑1 (MQ=255)
                                                                                                       gccccGCCCTCCAGCCGCGAACGCCCCGAACCTACCCGGTAAAACCGCTCGAACAAGCGCGACTGGTGCTCTGCAGGCACACCCGGACCCTGGTTCTCCACGGATAGAATGACTTCGCTGCCCGAGCGTTCGATgc                           <  7:380124/134‑1 (MQ=255)
                                                                                                       gTCCCGCCCTCCAGCCGCGAACGCCCCGAACCTCCCCGGTAAAACCGCTCGAACAAGCGCGACTGGTGCTCTGCAGGCACACCCGGACCCTGGTTCTCCACGGATAGAATGACTTCGCTGCCCGAGCGTTCGATgc                           <  4:122978/136‑1 (MQ=255)
                                                                                                          ccGCCCTCCAGCCGCGAACGCCCCGAACCTACCCGGTAAAACCGCTCGAACAAGCGCGACTGGTGCTCTGCAGGCACACCCGGACCCTGGTTCTCCACGGATAGAATGACTTCGCTGCCCGAGCGTTCGATgcg                          >  5:348155/1‑134 (MQ=255)
                                                                                                           cGCCCTCCAGCCGCGAACGCCCCGAACCTACCCGGTAAACCCGCTCGCACAAGCGCGCCTGGTGCTCTGCAGGCACCCCCGGCCCCTGGTGCGCCACGGATAGAGTGCCTTCGCTGCCCGAGCGTTCGATGCGCag                       >  3:372318/1‑136 (MQ=255)
                                                                                                           cGCCCTCCAGCCGCGAACGCCCCGAACCTACCCGGTAAAACCGCTCGAACAAGCGCGCCTGGTGCTCTGCAGGCACACCCGGACCCTGGTTCTCCACGGATAGAATGACTTCGCTGCCCGAGCGTTCGATGCGCag                       >  7:263036/1‑136 (MQ=255)
                                                                                                             ccgtCCAGCCGCGAACGCCGCGACCAAACCGGGTAAACCCGCTCGACCACGCGCGACTGGTGCTCTGCAGGCTCAACCGGACCCTGGTTCTCCACGGATAGAATGACTTCGCTGCCCGAGCGTTCGATGCGCagag                     <  4:397257/133‑1 (MQ=255)
                                                                                                                    gccgccACCGCCCCGACCCACCCCGGAAAACCCGCGCCACCACCCGCGACTGGTGCGCTGCAGGCACACCCGGACCCTGGTTCTCCACGGATAGAATGACTTCGCTGCCCGAGCGTTCGATGCGCAGAGCCACggg              <  6:237404/136‑1 (MQ=255)
                                                                                                                    gCCGCGACCGCCCCGAACCTACCCGGTAAAACCGCTCGAACAAGCGCGACTGGTGCTCTGCAGGCACACCCGGACCCTGGTTCTCCACGGATAGAATGACTTCGCTGCCCGAGCGTTCGATGCGCAGAGCCACggg              <  4:99984/136‑1 (MQ=255)
                                                                                                                    gCCGCGACCGCCCCGAACCTACCCGGTAAAACCGCTCGAACAAGCGCGACTGGTGCTCTGCAGGCACACCCGGACCCTGGTTCTCCACGGATAGAATGACTTCGCTGCCCGAGCGTTCGATGCGCAGAGCCACggg              <  1:261547/136‑1 (MQ=255)
                                                                                                                    gCCGCGAACCCCCCGAACCTACCCGGTAAAACCGCGCGAACACGCGCGACTGGTGCTCTGCAGGCACACCCGGACCCTGGTTCTCCACGGATAGAATGACTTCGCTGCCCGAGCGTTCGATGCGCAGAGCCACggg              <  1:370259/136‑1 (MQ=255)
                                                                                                                    cccGCGAACGCCCCGAACCTACCCGGTAAAACCGCTCGAACAAGCGCGACTGGTGCTCTGCAGGCACACCCGGACCCTGGTTCTCCACGGATAGAATGACTTCGCTGCCCGAGCGTTCGATGCGCAGAGCCACggg              <  8:353233/135‑1 (MQ=255)
                                                                                                                         gAACGCCCCGAACCTACCCGGTAAAACCGCTCTAACAAGCGCGACTGGTGCTCTGCAGGCACACCCGGACCCTGGTTCTCCACGGATAGAATGACTTCGCTGCCCGAGCGTTCGATGCGCAGAGCCACGGGTTTAc         <  2:297655/136‑1 (MQ=255)
                                                                                                                         caaCGCCCCCAACCACCCCGTAAAAACCGCTCGAACACGCGCGACTGGTGCTCTGCAGGCACACCCGGACCCTGGTTCTCCACGGATAGAATGACTTCGCTGCCCGAGCGTTCGATGCGCAGAGCCACGGGTTTAc         <  5:51312/135‑1 (MQ=255)
                                                                                                                           aCGCCCCGAACCTACCCGGTAAAACCGCTCGAACAAGCGCGACTGGTGCTCTGCAGGCACACCCGGACCCTGGTTCTCCACGGATAGAATGACTTCGCTGCCCGAGCGTTCGATGCGCAGAGCCACGGGTTTAccc       >  2:37681/1‑136 (MQ=255)
                                                                                                                             gCCCCGAACCTACCCGGTAAAACCGCTCGAACAAGCGCGACTGGTGCTCTGCAGGCACACCCGGACCCTGGTTCTCCACGGATAGAATGACTTCGCTGCCCGAGCGTTCGATGCGCAGAGCCACGTGTTTACCCTc     >  3:269482/1‑136 (MQ=255)
                                                                                                                              ccccGAACCTACCCGGTAAAACCGCGCGAACAAGCGCGACTGGTGCTCTGCAGGCACACCCGGACCCTGGTTCTCCACGGATAGAATGACTTCGCTGCCCGAGCGTTCGATGCGCAGAGCCACGGGTTTACCCTCg    <  6:101908/136‑1 (MQ=255)
                                                                                                                                ccGAACCAACCCGGTAAAACCGCTCGAACACGCGCGACTGGTGCTCTGCAGGCACACCCGGACCCTGGTTCTCCACGGATAGAATGACTTCGCTGCCCGAGCGTTCGATGCGCAGAGCCACGGGTTTACCCTCggg  <  4:153574/136‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                
AAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTGCATGATCGACTTCACGATCGCCAACCCCAACCCGGTCCCGCCCTCCAGCCGCGAACGCCCCGAACCTACCCGGTAAAACCGCTCGAACAAGCGCGACTGGTGCTCTGCAGGCACACCCGGACCCTGGTTCTCCACGGATAGAATGACTTCGCTGCCCGAGCGTTCGATGCGCAGAGCCACGGGTTTACCCTCGGG  >  ACB143/1630425‑1630688

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑