Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 1,699,121 C→T 5.6% R44C (CGT→TGT)  CJ019_01595 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1431,699,1210CT 5.6% 244.1 / 4.1 71R44C (CGT→TGT) CJ019_01595hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (38/29);  new base T (2/2);  total (40/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

TCGCATTGAAGCCGACTACGCGAACATTCTCTGAGCACTGCCGCCTGCTCGTCCTCGGCCTGGCCCTGCTGAGCCTTGGCGGCTGCAGCAGCCTGTTGTTCTACCCCGAGCGTGGTCAGGCCTTCACCCCTGAGCGTGCCAAGCTGGAATACCGCGATGTCACGCTGACCACTGCTGATGGCATCCGCCTTCACGGTTGGTGGCTGCCAGCCAAGGCCGGGGTCGAGGTCAAGGGCACGGTGCTGCACCTTCACGGCAACGGCGGTAA  >  ACB143/1698987‑1699254
                                                                                                                                      |                                                                                                                                     
tCGCATTGAAGCCGACTACGCGAACATTCTCTGAGCACTGCCGCCTGCTCGTCCTCGGCCTGGCCCTGCTGAGCCTTGGCGGCTGCAGCAGCCTGTTGTTCTACCCCGAGCGTGGTCAGGCCTTCACCCCTGAGCg                                                                                                                                      <  2:83045/136‑1 (MQ=255)
 cGCATTGAAGCCGACTACGCGAACATTCTCTGAGCACTGCCGCCTGCTCGTCCTCGGCCTGGCCCTGCTGAGCCTTGGCGGCTGCAGCAGCCTGTTGTTCTACCCCGAGCGTGGTCAGGCCTTCACCCCTGAGCGt                                                                                                                                     >  1:403042/1‑136 (MQ=255)
      tGAAGCCGACTACGCGAACATTCTCTGAGCACTGCCGCCTGCTCGTCCTCGGCCTGGCCCTGCTGAGCCTTGGCGGCTGCAGCAGCCTGTTGTTCTACCCCGAGCGTGGTCAGGCCTTCACCCCTGAGTGTGcc                                                                                                                                  >  8:220398/1‑134 (MQ=255)
      tGAAGCCGACTACGCGAACATTCTCTGAGCACTGCCGCCTGCTCGTCCTCGGCCTGGCCCTGCTGAGCCTTGGCGGCTGCAGCAGCCTGTTGTTCTACCCCGAGCGTGGTCAGGCCTTCACCCCTGAGTGTGCCaa                                                                                                                                >  4:134188/1‑136 (MQ=255)
          gCCGACTACGCGAACATTCTCTGAGCACTGCCGCCTGCTCGTCCTCGGCCTGGCCCTGCTGAGCCTTGGCGGCTGCAGCAGCCTGTTGTTCTACCCCGAGCGTGGTCAGGCCTTCACCCCTGAGCGTGCCAAGCTg                                                                                                                            <  7:30667/136‑1 (MQ=255)
                 aCGCGAACATTCTCTGAGCACTGCCGCCTGCTCGTCCTCGGCCTGGCCCTGCTGAGCCTTGGCGGCTGCAGCAGCCTGTTGTTCTACCCCGAGCGTGGTCAGGCCTTCACCCCTGAGCGTGCCAAGCTGGAATAcc                                                                                                                     >  4:227420/1‑136 (MQ=255)
                 aCGCGAACATTCTCTGAGCACTGCCGCCTGCTCGTCCTCGGCCTGGCCCTGCTGAGCCTTGGCGGCTGCAGCAGCCTGTTGTTCTACCCCGAGCGTGGTCAGGCCTTCACCCCTGAGCGTGCCAAGCTGGAATAcc                                                                                                                     >  7:80634/1‑136 (MQ=255)
                 aCGCGAACATTCTCTGAGCACTGCCGCCTGCTCGTCCTCGGCCTGGCCCTGCTGAGCCTTGGCGGCTGCAGCAGCCTGTTGTTCTACCCCGAGCGTGGTCAGGCCTTCACCCCTGAGCGTGCCAAGCTAGAATAcc                                                                                                                     >  2:383250/1‑136 (MQ=255)
                 aCGCGAACATTCTCTGAGCACTGCCGCCTGCTCGTCCTCGGCCTGGCCCTGCTGAGCCTTGGCGGCTGCAGCAGCCTGTTGTTCTACCCCGAGCGTGGTCAGGCCTTCACACCTGAGCGTGCCAAGCTGGAATAcc                                                                                                                     >  1:107573/1‑136 (MQ=255)
                     gAACATTCTCTGAGCACTGCCGCCTGCTCGTCCTCGGCCTGGCCCTGCTGAGCCTTGGCGGCTGCAGCAGCCTGTTGTTCTACCCCGAGCGTGGTCAGGCCTTCACCCCTGAGCGTGCCAAGCTGGAATACCGCGa                                                                                                                 <  1:29535/136‑1 (MQ=255)
                         aTTCTCTGAGCACTGCCGCCTGCTCGTCCTCGGCCTGGCCCTGCTGAGCCTTGGCGGCTGCAGCAGCCTGTTGTTCTACCCCGAGCGTGGTCAGGCCTTCACCCCTGAGCGTGCCAAGCTGGAATACCGCGATGTc                                                                                                             <  1:213314/136‑1 (MQ=255)
                         aTTCTCTGAGCACTGCCGCCTGCTCGTCCTCGGCCTGGCCCTGCTGAGCCTTGGCGGCTGCAGCAGCCTGTTGTTCTACCCCGAGCGTGGTCAGGCCTTCACCCCTGAGCGTGCCAAGCTGGAATACCGCGATGTc                                                                                                             <  3:338452/136‑1 (MQ=255)
                         aTTCTCTGAGCACTGCCGCCTGCTCGTCCTCGGCCTGGCCCTGCTGAGCCTTGGCGGCTGCAGCAGCCTGTTGTTCTACCCCGAGCGTGGTCAGACCTTCACCCCTGAGCGTGCCAAGCTGGAATACCGCGATGTc                                                                                                             <  5:73948/136‑1 (MQ=255)
                                 aGCACTGCCGCCTGCTCGTCCTCGGCCTGGCCCTGCTGAGCCTTGGCGGCTGCAGCAGCCTGTTGTTCTACCCCGAGCGTGGTCAGGCCTTCACCCCTGAGCGTGCCAAGCTGGAATACCGCGATGTCACGCt                                                                                                        <  6:123788/133‑1 (MQ=255)
                                 aGCACTGCCGCCTGCTCGTCCTCGGCCTGGCCCTGCTGAGCCTTGGCGGCTGCAGCAGCCTGTTGTTCTACCCCGAGCGTGGTCAGGCCTTCACCCCTGAGCGTGCCAAGCTGGAATACCGCGATGTCACGCTGa                                                                                                      <  1:266157/135‑1 (MQ=255)
                                 aGCACAGCCGCCTGCGCGTCCTCGGCCTGGCCCTGCTGAGCCTTGGCGGCTGCAGCAGCCTGCTGTTCTACCCCGAGCGTGGTCAGGCCTTCACCCCTGAGCGTGCCAAGCGGGAATACCGCGATGTCACGCTGa                                                                                                      <  1:109517/135‑1 (MQ=255)
                                    aCTGCCGCCTGCTCGTCCTCGGCCTGGCCCTGCTGAGCCTTGGCGGCTGCAGCAGCCTGTTGTTCTACCCCGAGCGTGGTCAGGCCTTCACCCCTGAGCGTGCCAAGCTGGAATACCGCGATGTCACGCTGACCAc                                                                                                  >  1:318298/1‑136 (MQ=255)
                                         cgccTGCTCGTCCTCGGCCTGGCCCTGCTGAGCCTTGGCGGCTGCAGCAGCCTGTTGTTCTACCCCGAGCGTGGTCAGGCCTTCACCCCTGAGCGTGCCAAGCTGGAATACCGCGATGTCACGCTGACCActgct                                                                                              >  8:276346/1‑135 (MQ=255)
                                         cgccTGCTCGTCCTCGGCCTGGCCCTGCTGAGCCTTGGCGGCTGCAGCAGCCTGTTGTTCTACCCCGAGCGTGGTCAGGCCTTCACCCCTGAGCGTGCCAAGCTGGAATACCGCGATGTCACGCTGACCActgct                                                                                              >  6:178804/1‑135 (MQ=255)
                                         cgccTGCTCGTCCTCGGCCTGGCCCTGCTGAGCCTTGGCGGCTGCAGCAGCCTGTTGTTCTACCCCGAGCGTGGTCAGGCCTTCACCCCTGAGCGTGCCAAGCTGGAATACCGCGATGTCACGCTGACCActgct                                                                                              >  1:108662/1‑135 (MQ=255)
                                              gCTCGTCCTCGGCCTGGCCCTGCTGAGCCTTGGCGGCTGCAGCAGCCTGTTGTTCTACCCCGAGCGTGGTCAGGCCTTCACCCCTGAGCGTGCCAAGCTGGAATACCGCGATGTCACGCTGACCACTGCTGATgg                                                                                         >  7:158532/1‑135 (MQ=255)
                                              gCTCGTCCTCGGCCTGGCCCTGCTGAGCCTTGGCGGCTGCAGCAGCCTGTTGTTCTACCCCGAGCGTGGTCAGGCCTTCACCCCTGAGCGTGCCAAGCTGGAATACCGCGATGTCACGCTGACCACTGCTGATgg                                                                                         >  1:63539/1‑135 (MQ=255)
                                              gCTCGTCCTCGGCCTGGCCCTGCTGAGCCTTGGCGGCTGCAGCAGCCTGTTGTTCTACCCCGAGCGTGGTCAGGCCTTCACCCCTGAGCGTGCCAAGCTGGAATACCGCGATGTCACGCTGACCACTGCTGATgg                                                                                         >  3:64821/1‑135 (MQ=255)
                                                tCGTCCTCGGCCTGGCCCTGCTGAGCCTTGGCGGCTGCAGCAGCCTGTTGTTCTACCCCGAGCGTGGTCAGGCCTTCACCCCTGAGCGTGCCAAGCTGGAATACCGCGATGTCACGCTGACCACTGCTGATGGCAt                                                                                      >  7:304330/1‑136 (MQ=255)
                                                tCGTCCTCGGCCTGGCCCTGCTGAGCCTTGGCGGCTGCAGCAGCCTGTTGTTCTACCCCGAGCGTGGTCAGGCCTTCACCCATGAGCGTGCCCAGCTGGAATACCGCGATGTCACGCTGACCACTGCTGCTGGCAt                                                                                      >  7:278564/1‑136 (MQ=255)
                                                        ggCCTGGCCCTGCTGAGCCTTGGCGGCTGCAGCAGCCTGTTGTTCTACCCCGAGCGTGGTCAGGCCTTCACCCCTGAGCGTGCCAAGCTGGAATACCGCGATGTCACGCTGACCACTGCTGATGGCATCCGCCt                                                                                <  5:178804/134‑1 (MQ=255)
                                                         gCCTGGCCCTGCTGAGCCTTGGCGGCTGCAGCAGCCTGTTGTTCTACCCCGAGCGTGGTCAGGCCTTCACCCCTGAGCGTGCCAAGCTGGAATACCGCGATGTCACGCTGACCACTGCTGATGGCATCCGCCTTCa                                                                             <  1:381279/136‑1 (MQ=255)
                                                              gCCCTGCTGAGCCTTGGCGGCTGCAGCAGCCTGTTGTTCTACCCCGAGCGTGGTCAGGCCTTCACCCCTGAGCGTGCCAAGCTGGAATACCGCGATGTCACGCTGACCACTGCTGATGGCATCCGCCTTCACgg                                                                          <  6:31531/134‑1 (MQ=255)
                                                                ccTGCTGAGCCTTGGCGGCTGCAGCAGCCTGTTGTTCTACCCCGAGCGTGGTCAGGCCTTCACCCCTGAGCGTGCCAAGCTGGAATACCGCGATGTCACGCTGACCACTGCTGATGGCATCCGCCTTCACGGTtgg                                                                      >  6:7185/1‑136 (MQ=255)
                                                                 ctgctgAGCCTTGGCGGCTGCAGCAGCCTGTTGTTCTACCCCGAGCGTGGTCAGGCCTTCACCCCTGAGCGTGCCAAGCTGGAATACCGCGATGTCACGCTGACCACTGCTGATGGCATCCGCCTTCACGGTtggt                                                                     >  1:358594/1‑136 (MQ=255)
                                                                    ctgAGCCTTGGCGGCTGCAGCAGCCTGTTGTTCTACCCCGAGCGTGGTCAGGCCTTCACCCCTGAGCGTGCCAAGCTGGAATACCGCGATGTCACGCTGACCACTGCTGATGGCATCCGCCTTCACGGTTGGTGGc                                                                  <  1:360532/136‑1 (MQ=255)
                                                                       aGCCTTGGCGGCTGCAGCAGCCTGTTGTTCAACCCCGAGCGTGGTCAGGCCTTCACCCCTGAGCGTGCCAAGCTGGAATACCGCGATGTCAAGCTGACCACTGCTGATGTCATCCGCCTTCACGGTGGGTGGCTgc                                                               >  7:331274/1‑136 (MQ=255)
                                                                         ccTTGGCGGCTGCAGCAGCCTGTTGTTCTACCCCGAGCGTGGTCAGGCCTTCACCCCTGAGCGTGCCAAGCTGGAATACCGCGATGTCACGCTGACCACTGCTGATGGCATCCGCCTTCACGGTTGGTGGCTgcca                                                             <  4:167771/136‑1 (MQ=255)
                                                                            tGGCGGCTGCAGCAGCCTGTTGTTCTACCCCGAGCGTGGTCAGGCCTTCACCCCTGAGCGTGCCAAGCTGGAATACCGCGATGTCACGCTGACCACTGCTGATGGCATCCGCCTTCACGGTTGGTGGCTgccagc                                                           >  5:282178/1‑135 (MQ=255)
                                                                            tGGCGGCTGCAGCAGCCTGTTGTTCTACCCCGAGCGTGGTCAGGCCTTCACCCCTGAGCGTGCCAAGCTGGAATACCGCGATGTCACGCTGACCACTGCTGATGGCATCCGCCTTCACGGTTGGTGGCTgccagc                                                           >  7:379225/1‑135 (MQ=255)
                                                                            tGGCGGCTGCAGCAGCCTGTTGTTCTACCCCGAGCGTGGTCAGGCCTTCACCCCTGAGCGTGCCAAGCTGGAATACCGCGATGTCACGCTGACCACTGCTGATGGCATCCGCCGTCACGGTTGGTGGCTgccagc                                                           >  1:3208/1‑135 (MQ=255)
                                                                               cggcTGCAGCAGCCTGTTGTTCTACCCCGAGCGTGGTCAGGCCTTCACCCCTGAGCGTGCCAAGCTGGAATACCGCGATGTCACGCTGACCACTGCTGATGGCATCCGCCt                                                                                <  2:375965/111‑1 (MQ=255)
                                                                               cggcTGCAGCAGCCTGTTGTTCTACCCCGAGCGTGGTCAGGCCTTCACCCCTGAGCGTGCCAAGCTGGAATACCGCGATGTCACGCTGACCACTGCTGATGGCATCCGCCt                                                                                >  1:375965/1‑111 (MQ=255)
                                                                                ggcTGCAGCAGCCTGTTGTTCTACCCCGAGCGTGGTCAGGCCTTCACCCCTGAGCGTGCCAAGCTGGAATACCGCGATGTCACGCTGACCACTGCTGATGGCATCCGCCTTCACGGTTGGTGGCTGCCAGCCAAgg                                                      >  7:215763/1‑136 (MQ=255)
                                                                                      agcagcCTGTTGTTCTACCCCGAGCGTGGTCAGGCGTTCCCCCATGCGCGTGCCAAGATGGATAACCGCGATGACACGATCCCCACTGCTGATGACATCCGCATTCAGGGTTGTTGGATCCCAGCCAGGGCCgggg                                                >  2:184222/1‑136 (MQ=255)
                                                                                               ttgttCTACCCCGAGCGTGGTCAGGCCTTCACCCCTGAGTGTGCCAAGCTGGAATACCGCGATGTCACGCTGACCACTGCTGATGGCATCCGCCTTCACGGTTGGTGGCTGCCAGCCAAGGCCGGGGTCGAGGt                                         <  3:134188/134‑1 (MQ=255)
                                                                                               ttgttCTACCCCGAGCGTGGTCAGGCCTTCACCCCTGAGTGTGCCAAGCTGGAATACCGCGATGTCACGCTGACCACTGCTGATGGCATCCGCCTTCACGGTTGGTGGCTGCCAGCCAAGGCCGGGGTCGAGGt                                         <  7:220398/134‑1 (MQ=255)
                                                                                                  ttCTACCCCGAGCGTGGTCAGGCCTTCACCCCTGAGCGTGCCAAGCTGGAATACCGCGATGTCACGCTGACCACTGCTGATGGCATCCGCCTTCACGGTTGGTGGCTGCCAGCCAAGGCCGGGGTCGAGGTCAAgg                                    <  5:228622/136‑1 (MQ=255)
                                                                                                  ttCTACCCCGAGCGTGGTCAGGCCTTCACCCCTGAGCGTGCCAAGCTGGAATACCGCGATGTCACGCTGACCACTGCTGATGGCATCCGCCTTCACGGTTGGTGGCTGCCAGCCAAGGCCGGGGTCGAGGTCAAgg                                    <  2:107573/136‑1 (MQ=255)
                                                                                                  ttCTACCCCGAGCGTGGTCAGGCCTTCACCCCTGAGCGTGCCAAGCTGGAATACCGCGATGTCACGCTGACCACTGCTGATGGCATCCGCCTTCACGGTTGGTGGCTGCCAGCCAAGGCCGGGGTCGAGGTCAAgg                                    <  1:63095/136‑1 (MQ=255)
                                                                                                  ttCTACCCCGAGCGTGGTCAGGCCTTCACCCCTGAGCGTGCCAAGCTGGAATACCGCGATGTCACGCTGACCACTGCTGATGGCATCCGCCTTCACGGTTGGTGGCTGCCAGCCAAGGCCGGGGTCGAGGTCAAgg                                    <  7:343038/136‑1 (MQ=255)
                                                                                                  ttCTACCCCGAGCGTGGTCAGGCCTTCACCCCTGAGCGTGCCAAGCTGGAATACCGCGATGTCACGCTGACCACTGCTGATGGCATCCGCCTTCACGGTTGGTGGCTGCCAGCCAAGGCCGGGGTCGAGGTCAAgg                                    <  7:243896/136‑1 (MQ=255)
                                                                                                  ttCTACCCCGAGCGTGGTCAGGCCTTCACCCCTGAGCGTGCCAAGCTGGAATACCGCGATGTCACGCTGACCACTGCTGATGGCATCCGCCTTCACGGTTGGTGGCTGCCAGCCAAGGCCGGGGTCGAGGTCAAgg                                    <  6:159832/136‑1 (MQ=255)
                                                                                                  ttCTACCCCGAGCGTGGTCAGGCCTTCACCCCTGAGCGTGCCAAGCTGGAATACCGCGATGTCACGCTGACCACTGCTGATGGCATCCGCCTTCACGGTTGGTGGCTGCCAGCCAAGGCCGGGGTCGAGGTCAAgg                                    <  5:159911/136‑1 (MQ=255)
                                                                                                  ttCTACCCCGAGCGTGGTCAGGCCTTCACCCCTGAGCGTGCCAAGCTGGAATACCGCGATGTCACGCTGACCACTGCTGATGGCATCCGCCTTCACGGTTGGTGGCTGCCAGCCAAGGCCGGGGTCGAGGTCAAgg                                    <  3:170349/136‑1 (MQ=255)
                                                                                                  gtCTACCCCGAGCGTGGTCAGGCCTTCACCCCTGAGCGGGCCAAGCTGGAATACCGCGATGTCACGCTGACCACTGCTGATGGCATCCGCCTTCACGGTTGGTGGCTGCCAGCCAAGGCCGGGGTCGAGGTCAAgg                                    <  8:96264/135‑1 (MQ=255)
                                                                                                    cTACCCCGAGCGTGGTCAGGCCTTCACCCCTGAGCGTGCCAAGCTGGAATACCGCGATGTCACGCTGACCACTGCTGATGGCATCCGCCTTCACGGTTGGTGGCTGCCAGCCAAGGCCGGGGTCGAGGTCAAggg                                   <  3:232557/135‑1 (MQ=255)
                                                                                                          cGAGCGTGGTCAGGCCTTCACCCCTGAGCGTGCCAAGCTGGAATACCGCGATGTCACGCTGACCACTGCTGATGGCATCCGCCTTCACGGTTGGTGGCTGCCAGCCAAGGCCggg                                                 >  6:85735/1‑115 (MQ=255)
                                                                                                          cGAGCGTGGTCAGGCCTTCACCCCTGAGCGTGCCAAGCTGGAATACCGCGATGTCACGCTGACCACTGCTGATGGCATCCGCCTTCACGGTTGGTGGCTGCCAGCCAAGGCCggg                                                 <  5:85735/115‑1 (MQ=255)
                                                                                                          cGAGCGTGGTCAGGCCTTCACCCCTGAGCGTGCCAAGCTGGAATACCGCGATGTCACGCTGACCACTGCTGATGGCATCCGCCTTCACGGTTGGTGGCTGCCAGCCAAGGCCGGGGTCGAGGTCAAGGGCACGGtg                            >  3:153806/1‑136 (MQ=255)
                                                                                                          cGAGCGTGGTCAGGCCTTCACCCCTGAGCGTGCCAAGCTGGAATACCGCGATGTCACGCTGACCACTGCTGATGGCATCCGCCTTCACGGTTGGTGGCTGCCAGCCAAGGCCGGGGTCGAGGTCAAGGGCACGGtg                            >  7:240041/1‑136 (MQ=255)
                                                                                                               gTGGTCAGGCCTTCATCCCTGAGCGTGCCAAGCTGGAATACCGCGATGTCACGCTGACCACTGCTGATGGCATCCGCCTTCACGGTTGGTGGCTGCCAGCCAAGGCCGGGGTCGAGGTCAAGGGCACGGTGCTGCa                       >  3:185251/1‑136 (MQ=255)
                                                                                                               gTGGTCAGGCCTTCACCCCTGAGCGTGCCAAGCTGGAATACCGCGATGTCACGCTGACCACTGCTGATGGc                                                                                        >  2:285711/1‑71 (MQ=255)
                                                                                                               gTGGTCAGGCCTTCACCCCTGAGCGTGCCAAGCTGGAATACCGCGATGTCACGCTGACCACTGCTGATGGc                                                                                        <  1:285711/71‑1 (MQ=255)
                                                                                                               gTGGTCAGGCCTTCACCCCTGAGCGTGCCAAGCTGGAATACCGCGATGTCACGCTGACCACTGCTGATGGCATCCGCCTTCACGGTTGGTGGCTGGCAGCCAAGGCCGGGGTCGAGGTCAAGGGCACTGTGCTGCa                       >  8:264906/1‑136 (MQ=255)
                                                                                                               gTGGTCAGGCCTTCACCCCTGAGCGTGCCAAGCTGGAATACCGCGATGTCACGCTGACCACTGCTGATGGCATCCGCCTTCACGGTTGGTGGCTGCCAGCCAAGGCCgggg                                                <  3:257024/111‑1 (MQ=255)
                                                                                                               gTGGTCAGGCCTTCACCCCTGAGCGTGCCAAGCTGGAATACCGCGATGTCACGCTGACCACTGCTGATGGCATCCGCCTTCACGGTTGGTGGCTGCCAGCCAAGGCCgggg                                                >  4:257024/1‑111 (MQ=255)
                                                                                                               gTGGTCAGGCCTTCACCCCTGAGCGTGCCAAGCTGGAATACCGCGATGTCACGCTGACCACTGCTGATGGCATCCGCCTTCACGGTTGGTGGCTGCCAGCCAAGGCCGGGGTCGAGGTCAAGGGCACGGTGCTGCa                       >  7:82412/1‑136 (MQ=255)
                                                                                                               gTGGTCAGGCCTTCACCCCTGAGCGTGCCAAGCTGGAATACCGCGATGTCACGCTGACCACTGCTGATGGCATCCGCCTTCACGGTTGGTGGCTGCCAGCCAAGGCCGGGGTCGAGGTCAAGGGCACGGTGCTGCa                       >  2:343782/1‑136 (MQ=255)
                                                                                                               gTGGTCAGGCCTTCACCCCTGAGCGTGCCAAGCTGGAATACCGCGATGTCACGCTGACCACTGCTGATGGCATCCGCCTTCACGGTTGGTGGCTGCCAGCCAAGGCCCGGGTCGAGGTCAAGGGCACGGTGCTGCa                       >  2:33213/1‑136 (MQ=255)
                                                                                                               gTGGTCAGGCCTTCACCCCTGAGCGTGCCAAGCTGGAATACCGCGATGTCACGATGACCACTGCTGATGGCATCCGCCTTCACTGTTGGTGGCTGCCAGCCAAGGCCGGGGTCGAGGTCAAGGTCACGGtgctgaa                       >  2:312678/1‑134 (MQ=255)
                                                                                                                        ccTTCACCCCTGAGCGTGCCAAGCTGGAATACCGCGATGTCACGCTGACCACTGCTGATGGCATCCGCCTTCACGGTTGGTGGCTGCCAGCCAAGGCCGGGGTCGAGGTCAAGGGCACGGTGCTGCACCTTCACgg              <  3:172785/136‑1 (MQ=255)
                                                                                                                                    aGCGTGCCAAGCTGGAATACCGCGATGTCACGCTGACCACTGCTGATGGCATCCGCCTTCACGGTTGGTGGCTGCCAGCCAAGGCCGGGGTCGAGGTCAAGGGCACGGTGCTGCCCCTTCACGGCAACGGCGGTaa  >  7:347447/1‑136 (MQ=255)
                                                                                                                                    aGCGTGCCAAGCTGGAATACCGCGATGTCACGCTGACCACTGCTGATGGCATCCGCCTTCACGGTTGGTGGCTGCCAGCCAAGGCCGGGGTCGAGGTCAAGGGCACGGTGCTGCACCTTCACGGCAACGGCGGTaa  >  7:43287/1‑136 (MQ=255)
                                                                                                                                    aGCGTGCCAAGCTGGAATACCGCGATGTCACGCTGACCACTGCTGATGGCATCCGCCTTCACGGTTGGTGGCTGCCAGCCAAGGCCGGGGTCGAGGTCAAGGGCACGGTGCTGCACCTTCACGGCAACGGCGGTaa  >  6:111702/1‑136 (MQ=255)
                                                                                                                                    aGCGTGCCAAGCTGGAATACCGCGATGTCACGCTGACCACTGCTGATGGCATCCGCCTTCACGGTTGGTGGCTGCCAGCCAAGGCCGGGGTCGAGGTCAAGGGCACGGTGCTGCACCTTCACGGCAACGGCGGTaa  >  3:151240/1‑136 (MQ=255)
                                                                                                                                      |                                                                                                                                     
TCGCATTGAAGCCGACTACGCGAACATTCTCTGAGCACTGCCGCCTGCTCGTCCTCGGCCTGGCCCTGCTGAGCCTTGGCGGCTGCAGCAGCCTGTTGTTCTACCCCGAGCGTGGTCAGGCCTTCACCCCTGAGCGTGCCAAGCTGGAATACCGCGATGTCACGCTGACCACTGCTGATGGCATCCGCCTTCACGGTTGGTGGCTGCCAGCCAAGGCCGGGGTCGAGGTCAAGGGCACGGTGCTGCACCTTCACGGCAACGGCGGTAA  >  ACB143/1698987‑1699254

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑