Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB143	1,721,874	A→C	29.8%	W97G (TGG→GGG)	CJ019_01617 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB143	1,721,874	0	A	C	29.8%	50.9 / 2.2	37	W97G (TGG→GGG)	CJ019_01617	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (21/4);  new base C (5/7);  total (26/11)
Fisher's exact test for biased strand distribution p-value = 1.83e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCGCGGGTAAACCCATTCCTACAGAAACGAGCAAGCCTGCCGTTTCATGATGTACGCCAGGTGATTTCCTCTTCACCGTCGGCGCTGATGCGGACCCAGCGGTCAGCATCTTCTTCCCCGTCTTCCTCGACCCAGCCACCCGGCGCGCAGCGCACTTCCACACCCAGCGCGGCAAATGCGGCGCGGGCGCAGGCAACGTCATCGGCCCACGGCGTCTGGTCGCTTTCCAGATACAGGCTGTTCCATTTCCCTACAGCCTTGGGTAACC  >  ACB143/1721741‑1722008                                                                                                                                      |                                                                                                                                       tCGCGGGTAAACCCATTCCTACAGAAACGAGCAAGCCTGCCGTTTCATGATGTACGCCAGGTGATTTCCTCTTCACCGTCGGCGCTGATGCGGACCCAGCGGTCAGCATCTTCTTCCCCGTCTTCCTCGACCCAGc                                                                                                                                      >  8:18235/1‑136 (MQ=255) tCGCGGGTAAACCCATTCCTACAGAAACGAGCAAGCCTGCCGTTTCATGATGTACGCCAGGTGATTTCCTCTTCACCGTCGGCGCTGATGCGGACCCAGCGGTCAGCATCTTCTTCCCCGTCTTCCTCGACCCAGc                                                                                                                                      >  1:283829/1‑136 (MQ=255) tCGCGGGTAAACCCATTCCTACAGAAACGAGCAAGCCTGCCGTTTCATGATGTACGCCAGGTGATTTCCTCTTCACCGTCGGCGCTGATGCGGACCCAGCGGTCAGCATCGTCTTCCCCGGCTTCCTCGACCCCGc                                                                                                                                      >  1:401913/1‑136 (MQ=255) tCGCGGGTAAACCCATTCCTACAGAAACGAGCAAGCCTGCCATTTCATGATGTACGCCAGGTGATTTCCTCTTCACCGTCGGCGCTGATGCGGACCCAGCGGTCCGCATCTTCTTCCCCGTCTTCCTCGACCCAGc                                                                                                                                      >  1:212523/1‑136 (MQ=255)  cgcgGGTAAACCCATTCCTACAGAAACGAGCAAGCCTGCCGTTTCATGATGTACGCCAGGTGATTTCCTCTTCACCGTCGGCGCTGATGCGGACCCAGCGGTCAGCATCTTCTTCCCCGTCTTCCTCGACCCAGc                                                                                                                                      <  1:273162/135‑1 (MQ=255)      ggTAAACCCATTCCTACAGAAACGAGCAAGCCTGCCGTTTCATGATGTACGCCAGGTGATTTCCTCTTCACCGTCGGCGCTGATGCGGACCCAGCGGTCAGCATCTTCTTCCCCGTCTTCCTCGACCCAGCCAccc                                                                                                                                 >  6:357879/1‑136 (MQ=255)       gTAAACCCATTCCTACAGAAACGAGCAAGCCTGCCGTTTCATGATGTACGCCAGGTGATTTCCTCTTCACCGTCGGCGCTGATGCGGACCCAGCGGTCAGCATCTTCTTCCCCGTCTTCCTCGACCCAGCCACCCg                                                                                                                                >  2:215590/1‑136 (MQ=255)       gTAAACCCATTCCTACAGAAACGAGCAAGCCTGCCGTTTCATGAAGTACGCCAGGTGATTTCCTATTCACCGTCGGCGCTGATGCGGACCCAGCGGTCATGAACTTCTTACCCATCTTCCTAGACCCAGCCAcccc                                                                                                                                >  2:282694/1‑135 (MQ=255)                    tACAGAAACGAGCAAGCCTGCCGTTTCATGATGTACGCCAGGTGATTTCCTCTTCACCGTCGGCGCTGATGCGGCCCCAGCGGTCAGCATCTTCTTCCCCGTCTTCCTCGACCCAGCCACCCGGCGCGCAGCGCAc                                                                                                                   >  5:273124/1‑136 (MQ=255)                    tACAGAAACGAGCAAGCCTGCCGTTTCATGATGTACGCCAGGTGATTTCCTCTTCACCGTCGGCGCTGATGCGGACCCAGCGGTCAGCATCTTCTTCCCCGTCTTCCTCGACCCCGCCACCCGGCgcgcagcgccc                                                                                                                   >  8:109291/1‑134 (MQ=255)                    tACAGAAACGAGCAAGCCTGCCGTTTCATGATGTACGCCAGGTGATTTCCTCTTCACCGTCGGCGCTGATGCGGACCCAGCGGTCAGCATCTTCATCCCCGTCTTCCTCGACCCCGCCACCCGGCgcgcagcgca                                                                                                                    >  2:387690/1‑135 (MQ=255)                    tACAGAAACGAGCAAGCCTGCCGTTTCATGATGTACGCCAGGTGATTTCCTCTTCACCGTCGGCGCTGATGCGGACCCAGCGGTCAGCATATTATTACCCGTCGTGCTCGCCCCAGCCCCCCGGCgcgccgcccca                                                                                                                   >  1:286617/1‑132 (MQ=255)                                 aaGCCTGCCGTTTCATGATGTACGCCAGGTGATTTCCTCTTCACCGTCGGCGCTGATGCGGACCCAGCGGTCAGCCTCTTCTTCCCCGTCTTCCTCGACCCCGCCACCCGGCGCGCAGCGCACATCCACACCCAgc                                                                                                      >  3:220684/1‑136 (MQ=255)                                 aaGCCTGCCGTTTCATGATGTACGCCAGGTGATTTCCTCTTCACCGTCGGCGCTGAAGCGGACCCAGCGGTCAGCATCTTCTTCCCCGTCTTACTAGACCCAGCCACCCGGCGCGCAGCGCACATACACACCCAgc                                                                                                      >  1:287198/1‑136 (MQ=255)                                                          atggTTATTACCTCTTCCCCGCCGGTGCGGTTGCGCACCCCGCGGGAACAATCTGCTACCCGTTCTCCCACGCCCCCCCCCCCGGGCGGGCCGGGCACTTCCACACCCAGCGCGGCAAATGCGGCGCGGGCGCAgg                                                                             <  6:198232/134‑1 (MQ=255)                                                               gATTTCCTCTTCACCGTCGGCGCTGATGCGGACCCAGCGGTCAGCATCTTCTTCCCCGTCTTCCTCGACCCCGCCACCCGGCGCGCAGCGCCCTTCCACACCCAGCGCGGCAAATGCGGCGCGGGCGCAGGCAACg                                                                        >  5:321737/1‑136 (MQ=255)                                                                aTTTCCTCTTCACCGTCGGCGCTGATGCGGACCCAGCGGTCAGCATCTTCTTCCCCGTCTTCCTCGACCCAGCCACCCGGCGCGCCGCGCACTTCCACACCCAGCGCGGCAAATGCGGCGCGGGCGCAGGCAACGt                                                                       >  1:317285/1‑136 (MQ=255)                                                                aTTTCCTCTTCACCGTCGGCGCTGATGCGGACCCAGCGGTCAGCATCTTCTTCCCCGTCTTCCTCGACCCAGCCACCCGGCGCGCAGCGCACTTCCACACCCAGCGCGGCAAATGCGGCGCGGGCGCAGGCAACGt                                                                       >  1:48565/1‑136 (MQ=255)                                                                  ttCCTCTTCACCGTCGGCGCTGATGCGGACCCAGCGGTCAGCATCTTCTTCCCCGTCTTCCTCGACCCAGCCACCCGGCGCGCCGCGCACTTCCACACCCAGCGCGGCAAATGCGGCGCGGGCGCAGGCAACGTCa                                                                     >  7:144757/1‑136 (MQ=255)                                                                          ccccGGCGGGGGTGAGGCGCCCCCCGCGGTCCCAATTTTCTCCCCCGTCGACCGCCCCCCCCCCCCCGGGCGCGCCCGCCACTTCCACACCCAGCGCGGCAAATGCGGCGCGGGCGCAGGCAACGTCATCGGc                                                                <  3:315320/131‑1 (MQ=255)                                                                                cGGCGCTGATGCGGACCCAGCGGTCAGCATCTTCTTCCCCGTCTTCCTCGACCCAGCCACCCGGCGCGCAGCGCACTTCCACACCCAGCGCGGCAAATGCGGCGCGGGCGCAGGCAACGTCATCGGCCCCCGGCgg                                                       >  2:141351/1‑135 (MQ=255)                                                                                  gcgcTGATGCGGACCCAGCGGTCAGCATCTTCTTCCCCGTCTTCCTCGACCCAGCCACCCGGCGCGCAGCGCACTTCCACACCCCGCGCGGCAAATGCGGCGCGGGCGCAGGCAACGTCATCGGCCCACGGCGTCt                                                     >  3:277275/1‑136 (MQ=255)                                                                                  gcgcTGATGCGGACCCAGCGGTCAGCATCTTCTTCCCCGTCTTCCTCGACCCAGCCACCCGGCGCGCAGCGCACTTCCACACCCAGCGCGGCAAATGCGGCGCGGGCGCAGGCAACGTCATCGGCCCACGGCGTCt                                                     >  1:31517/1‑136 (MQ=255)                                                                                                 cAGCGGTCAGCATCTTCTTCCCCGTCTTCCTCGACCCAGCCACCCGGCGCGCAGAGCACTTCCACACCCAGCGCGGCAAATGCGGCGCGGGCGCAGGCAACGTCATCGGCCCACGGCGTCTGGTCGCTTTCCAGAt                                      <  5:357879/136‑1 (MQ=255)                                                                                                     ggTCAGCATCTTCTTCCCCGTCTTCCTCGACCCAGCCACCCGGCGCGCAGCGCACTTCCACACCCAGCGCGGCAAATGCGGCGCGGGCGCAGGCAACGTCATCGGCCCACGGCGTCTGGTCGCTTTCCAGATACAg                                  >  2:89823/1‑136 (MQ=255)                                                                                                           cATCTTCTCCCCCGTCTCCCTCCCCCCCCCCCCCCGGCGCGCCGCGCACTTCCACACCCAGCGCGGCAAATGCGGCGCGGGCGCAGGCAACGTCATCGGCCCACGGCGTCTGGTCGCTTTCCAGATACAGGCTGtt                            <  8:232951/136‑1 (MQ=255)                                                                                                           attcttccTCCCCGTGTCCCCCCCCCCCGCCCCCGGGCGCGCCGCGCACTTCCACACCCAGCGCGGCAAATGCGGCGCGGGCGCAGGCAACGTCATCGGCCCACGGCGTCTGGTCGCTTTCCAGATACAGGCTGtt                            <  7:224215/134‑1 (MQ=255)                                                                                                           aaTCTTCTCCCCCGTATCCCTCCCCCCCCCCCCCCGGCGCCCAGCGCACTTCCACACCCAGCGCGGCAAATGCGGCGCGGGCGCAGGCAACGTCATCGGCCCACGGCGTCTGGTCGCTTTCCAGATACAGGCTGtt                            <  8:36757/135‑1 (MQ=255)                                                                                                            tttttcttcCCCGGCATCCTCCCCCCAGCCCCCCGGCGCGCCGCCCACTTCCACACCCAGCGCGGCAAATGCGGCGCGGGCGCAGGCAACGTCATCGGCCCACGGCGTCTGGTCGCTTTCCAGATACAGGCTGTTc                           <  7:294888/133‑1 (MQ=255)                                                                                                            ttcttctCCCCGGTCTTCCTCCACCCCCCCCCCCGCCGCGCCCCGCACTTCCACACCCAGCGCGGCAAATGCGGCGCGGGCGCAGGCAACGTCATCGGCCCACGGCGTCTGGTCGCTTTCCAGATACAGGCTGtt                            <  6:298807/134‑1 (MQ=255)                                                                                                            aTCTTCTCCCCCGTCTCCCACCCCCCCCCCCCCCGGCGCGCCCCCCACTTCCACACCCAGCGCGGCAAATGCGGCGCGGGCGCAGGCAACGTCATCGGCCCACGGCGTCTGGTCGCTTTCCAGATACAGGCTGTTc                           <  4:180044/136‑1 (MQ=255)                                                                                                                  ttcCCCGTCTTCCTCGACCCAGCCACCCGGCGCGCAGCGCACTTCCACACCCAGCGCGGCAAATGCGGCGCGGGCGCAGGCAACGTCATCGGCCCACGGCGTCTGGTCGCTTTCCAGATACAGGCTGTTCCATGTc                     >  5:144674/1‑136 (MQ=255)                                                                                                                   tcCCCGTCTTCCTCGACCCAGCCACCCGGCGCGCAGCGCACTTCCACACCCAGCGCGGCAAATGCGGCGCGGGCGCAGGCAACGTCATCGGCCCACGGCGTCTGGTCGCTTTCCAGATACAGGCTGTTCCATTTcc                    >  7:305001/1‑136 (MQ=255)                                                                                                                     cccGTCTCCCCCCCCCCACCCCCCGGGCGCCCCGCCCGCTTCCACACCCAGCGCGGCAAATGCGGCGCGGGCGCAGGCAACGTCATCGGCCCACGGCGTCTGGTCGCTTTCCAGATACAGGCTGTTCCATTTcc                    <  2:365665/134‑1 (MQ=255)                                                                                                                            tCCTCGACCCAGCCACCCGGCGCGCAGCGCACTTCCACACCCAGCGCGGCAAATGCGGCGCGGGCGCAGGCAACGTCATCGGCCCACGGCGTCTGGTCGCTTTCCAGATACAGGCTGTTCCATTTCCCTACAGCCt           >  5:297078/1‑136 (MQ=255)                                                                                                                            tCCTCGACCCAGCCACCCGGCGCGCAGCGCACTTCCACACCCAGCGCGGCAAATGCGGCGCGGGCGCAGGCAACGTCATCGGCCCACGGCGTCTGGTCGCTTTCCAGATACAGGCTGTTCCATTTCCCTACAGCCt           >  1:33660/1‑136 (MQ=255)                                                                                                                                   cccAGCCACCCGGCGCGCAGCGCACTTCCACACCCAGCGCGGCAAATGCGGCGCGGGCGCAGGCAACGTCATCGGCCCACGGCGTCTGGTCGCTTTCCAGATACAGGCTGTTCCATTTCCCTACAGCCTTGGGTaa    >  4:42888/1‑136 (MQ=255)                                                                                                                                      aGCCACCCGGCGCGCAGCGCACTTCCACACCCAGCGCGGCAAATGCGGCGCGGGCGCAGGCAACGTCATCGGCCCACGGCGTCTGGTCGCTTTCCAGATACAGGCTGTTCCATTTCCCTACAGCCTTGGGTAAcc  >  1:25804/1‑135 (MQ=255)                                                                                                                                      |                                                                                                                                       TCGCGGGTAAACCCATTCCTACAGAAACGAGCAAGCCTGCCGTTTCATGATGTACGCCAGGTGATTTCCTCTTCACCGTCGGCGCTGATGCGGACCCAGCGGTCAGCATCTTCTTCCCCGTCTTCCTCGACCCAGCCACCCGGCGCGCAGCGCACTTCCACACCCAGCGCGGCAAATGCGGCGCGGGCGCAGGCAACGTCATCGGCCCACGGCGTCTGGTCGCTTTCCAGATACAGGCTGTTCCATTTCCCTACAGCCTTGGGTAACC  >  ACB143/1721741‑1722008

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑