Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 1,838,093 C→T 8.3% D31D (GAC→GAT)  cseB → Transcriptional regulatory protein CseB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1431,838,0930CT 8.3% 152.2 / 3.8 48D31D (GAC→GAT) cseBTranscriptional regulatory protein CseB
Reads supporting (aligned to +/- strand):  ref base C (17/27);  new base T (2/2);  total (19/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.27e-01

TGCCGAGGCATTGGTCGCAGTGCGGTTGCCTGGCAGGTGGCGAATGCTCAATAATCACGCAATTGAGAATGCATGGCAGTTGCCAATGAATCCTGTATCTATTTACAACACCAACATCCTTGCCATCGAGGACGACCCGGTGCTTGGTGCCTATCTGCAGGAAGAGCTGCAGCGTGGCGGCTGCCAGGTCACCTGGTGCCGCAATGGCCTGGAGGGCCTGGAAACGGCTGGCCGCCAGGCTTTCGATGTGGTGCTGATGGACATCCTGCTG  >  ACB143/1837958‑1838228
                                                                                                                                       |                                                                                                                                       
tGCCGAGGCATTGGTCGCAGTGCGGTTGCCTGGCAGGTGGCGAATGCTCAATAATCACGCAATTGAGAATGCATGGCAGTTGCCAATGAATCCTGTATCTATTTACAACACCAACATCCTTGCCATCGAGgacgac                                                                                                                                         >  7:306141/1‑136 (MQ=255)
tGCCGAGGCATTGGTCGCAGTGCGGTTGCCTGGCAGGTGGCGAATGCTCAATAATCACGCAATTGAGAATGCATGGCAGTTGCCAATGAATCCTGTATCTATTTACAACACCAACATCCTTGCCATCGAGgacgac                                                                                                                                         >  7:311662/1‑136 (MQ=255)
  ccGAGGCATTGGTCGCAGTGCGGTTGCCTGGCAGGTGGCGAATGCTCAATAATCACGCAATTGAGAATGCATGGCAGTTGCCAATGAATCCTGTATCTATTTACAACACCAACATCCTTGCCATCGAGGACGAccc                                                                                                                                       >  2:321452/1‑136 (MQ=255)
  ccGAGGCATTGGTCGCAGTGCGGTTGCCTGGCAGGTGGCGAATGCTCAATAATCACGCAATTGAGAATGCATGGCAGTTGCCAATGAATCCTGTATCTATTTACAACACCAACATCCTTGCCATCGAGGACGAccc                                                                                                                                       >  3:244111/1‑136 (MQ=255)
     aGGCATTGGTCGCAGTGCGGTTGCCTGGCAGGTGGCGAATGCTCAATAATCACGCAATTGAGAATGCATGGCAGTTGCCAATGAATCCTGTATCTATTTACAACACCAACATCCTTGCCATCGAGGACGACCCGGt                                                                                                                                    <  1:39098/136‑1 (MQ=255)
     aGGCATTGGTCGCAGTGCGGTTGCCTGGCAGGTGGCGAATGCTCAATAATCACGCAATTGAGAATGCATGGCAGTTGCCAATGAATCCTGTATCTATTTACAACACCAACATCCTTGCCATCGAGGACGACCCGGt                                                                                                                                    <  3:311311/136‑1 (MQ=255)
                        gTTGCCTGGCAGGTGGCGAATGCTCAATAATCACGCAATTGAGAATGCATGGCAGTTGCCAATGAATCCTGTATCTATTTACAACACCACCATCCTTGCCATCGAGGACGACCCGGTGCTTGGTGCCTATCTGCAg                                                                                                                 <  3:367392/136‑1 (MQ=255)
                            ccTGGCAGGTGGCGAATGCTCAATAATCACGCAATTGAGAATGCATGGCAGTTGCCAATGAATCCTGTATCTATTTACAACACCAACATCCTTGCCATCGAGGACGACCCGGTGCTTGGTGCCTATCTGCAGGaa                                                                                                              <  8:302714/135‑1 (MQ=255)
                                 cAGGTGGCGAATGCTCAATAATCACGCAATTGAGAATGCATGGCAGTTGCCAATGAATCCTGTATCTATTTACAACACCAACATCCTTGCCATCGAGGACGACCCGGTGCTTGGTGCCTATCTGCAGGAAGAGCTg                                                                                                        >  8:194639/1‑136 (MQ=255)
                                 cAGGTGGCGAATGCTCAATAATCACGCAATTGAGAATGCATGGCAGTTGCCAATGAATCCTGTATCTATTTACAACACCAACATCCTTGCCATCGAGGACGACCCGGTGCTTGGTGCATATCTGCAGGAAGAGCTg                                                                                                        >  8:319170/1‑136 (MQ=255)
                                                 aataatCACGCAATTGAGAATGCATGGCAGTTGCCAATGAATCCTGTATCTATTTACAACACCAACATCCTTGCCATCGAGGACGACCCGGTGCTTGGTGCCTATCTGCAGGAAGAGCTGCAGCGTGGCGGCTGc                                                                                         >  8:55231/1‑135 (MQ=255)
                                                      tCACGCAATTGAGAATGCATGGCAGTTGCCAATGAATCCTGTATCTATTTACAACACCAACATCCTTGCCATCGAGGACGACCCGGTGCTTGGTGCCTATCTGCAGGAAGAGCTGCAGCGTGGCGGCTGCCAGGTc                                                                                   <  7:260937/136‑1 (MQ=255)
                                                           cAATTGAGAATGCATGGCAGTTGCCAATGAATCCTGTATCTATTTACAACACCAACATCCTTGCCATCGAGGACGACCCGGTGCTTGGTGCCTATCTGCAGGAAGAGCTGCAGCGTGGCGGCTGCCAGGTCACCTg                                                                              <  5:94010/136‑1 (MQ=255)
                                                                gagaATGCACGGCAGTTGCCAATGAATCCTGTATCTAATTACAACACCCACATCCTTGCCATCGAGGACGACCCGGTGCTTGGTGCCTATCTGCAGGAAGAGCTGCAGCGTGGCGGCTGCCAGGTCACCTGGTGcc                                                                         <  8:107611/136‑1 (MQ=255)
                                                                 agaATGCATGTCAGTTGCCAATGAATCCTGTATCTATTTACAACACCAACATCCTTGCAATAGAGGACGACCCTGTGCTTGGTGCCTATCTGCAGGAAGAGCTGCAGCGTGGCGGCTGCCAGGTCACCTGGTGcc                                                                         <  1:312614/135‑1 (MQ=255)
                                                                 agaATGCATGGCAGTTGCCAATGAATCCTGTATCTATTTACAACACCAACATCCTTGCCATCGAGGACGACCCGGTGCTTGGTGCCTATCTGCAGGAAGAGCTGCAGCGTGGCGGCTGCCAGGTCACCTGGTGcc                                                                         <  8:107479/135‑1 (MQ=255)
                                                                 agaATGCATGGCAGTTGCCAATGAATCCTGTATCTATTTACAACACCAACATCCTTGCCATCGAGGACGACCCGGTGCTTGGTGCCTATCTGCAGGAAGAGCTGCAGCGTGGCGGCTGCCAGGTCACCTGGTGcc                                                                         <  3:399636/135‑1 (MQ=255)
                                                                 agaATGCATGGCAGTTGCCAATGAATCCTGTATCTATTTACAACACCAACATCCTTGCCATCGAGGACGACCCGGTGCTTGGTGCCTATCTGCAGGAAGAGCTGCAGCGTGGCGGCTGCCAGGTCACCTGGTGcc                                                                         <  2:385020/135‑1 (MQ=255)
                                                                 agaATGCATGGCAGTTGCCAATGAATCCTGTATCTATTAACAACACCAACATCCTTGCCCTCGAGGACGACCCGGTGCTTGGTGCCTATCTGCAGGAAGAGCTGCAGCGTGGCGGCTGCCAGGTCACCTGGTGcc                                                                         <  5:28955/135‑1 (MQ=255)
                                                                 agaATGCATGGCAGTTGCAAATGAATCCTGTATCTAATAACAACACAAACATCCTTGCCATCGAGGACGACCCGGTGCTTGGTGCCTATCTGCATGAAGAGCTGCAGCGTGGCGGCTGCCAGGACACCTGGTGcc                                                                         <  8:377826/135‑1 (MQ=255)
                                                                         tGGCAGTTGCCAATGAATCCTGTATCTATTTACAACACCAACATCCTTGCCATCGAGGACGACCCGGTGCTTGGTGCCTATCTGCAGGAAGAGCTGCAGCGTGGCGGCTGCCAGGTCACCTGGTGCCGCAATGGcc                                                                >  5:156782/1‑136 (MQ=255)
                                                                           gCAGTTGCCAATGAATCCTGTATCTATTTACAACACCAACATCCTTGCCATCGAGGACGACCCGGTGCTTGGTGCCTATCTGCAGGAAGAGCTGCAGCGTGGCGGCTGCCAGGTCACCTGGTGCCGCAATGGCCt                                                               <  1:321452/135‑1 (MQ=255)
                                                                           gCAGTTGCCAATGAATCCTGTATCTATTTACAACACCAACATCCTTGCCATCGAGGACGACCCGGTGCTTGGTGCCTATCTGCAGGAAGAGCTGCAGCGTGGCGGCTGCCAGGTCACCTGGTGCCGCAATGGCCTg                                                              >  3:216599/1‑136 (MQ=255)
                                                                           gCAGTTGCCAATGAATCCTGTATCTATTTACAACACCAACATCCTTGCCATCGAGGACGACCCGGTGCTTGGTGCCTATCTGCAGGAAGAGCTGCAGCGTGGCGGCTGCCAGGTCACCTGGTGCCGCAATGGCCTg                                                              >  8:178900/1‑136 (MQ=255)
                                                                                  ccAATGAATCCTGTATCTATTTACAACACCAACATCCTTGCCATCGAGGACGACCCGGTGCTTGGTGCCTATCTGCAGGAAGAGCTGCAGCGTGGCGGCTGCCAGGTCACCTGGTGCCGCAATGGCCTGGAGGGcc                                                       >  6:201373/1‑136 (MQ=255)
                                                                                    aaTGAATCCTGTATCTATTTACAACACCAACATCCTTGCCATCGAGGACGACCCGGTGCTTGGTGCCTATCTGCAGGAAGAGCTGCAGCGTGGCGGCTGCCAGGTCACCTGGTGCCGCAATGGCCTGGAGGGCCTg                                                     >  5:284420/1‑136 (MQ=255)
                                                                                          tCCTGTATCTATTTACAACACCAACATCCTTGCCATCGCGGACGACCCGGTGCTTGGTGCCTATCTGCAGGAAGAGCTGCAGCGTGGCGGCTGCCAGGTCACCTGGTGCCGCAATGGCCTGGAGGGCCTGGAAACg                                               <  6:120619/136‑1 (MQ=255)
                                                                                             tGTATCTATTTACAACACCAACATCCTTGCCATCGAGGACGACCCGGTGCTTGGTGCCTATCTGCAGGAAGAGCTGCAGCGTGGCGGCTGCCAGGTCACCTGGTGCCGCAATGGCCTGGAGGGCCTGGAAACGGCt                                            >  8:290314/1‑136 (MQ=255)
                                                                                                       tACAACACCAACATCCTTGCCATCGAGGACGACCCGGTGCTTGGTGCCTATCTGCAGGAAGAGCTGCAGCGTGGCGGCTGCCAGGTCACCTGGTGCCGCAATGGCCTGGAGGGCCTGGACACGGCTGGCCGCCAgg                                  >  5:278678/1‑136 (MQ=255)
                                                                                                       tACAACACCAACATCCTTGCCATCGAGGACGACCCGGTGCTTGGTGCCTATCTGCAGGAAGAGCTGCAGCGTGGCGGCTGCCAGGTCACCTGGTGCCGCAATGGCCTGGAGGGCCTGGAAACGGCTGGCCGCCAgg                                  >  4:9872/1‑136 (MQ=255)
                                                                                                       tACAACACCAACATCCTTGCCATCGAGGACGACCCGGTGCTTGGTGCCTATCTGCAGGAAGAGCTGCAGCGTGGCGGCTGCCAGGTCACCTGGTGCCGCAATGGCCTGGAGGGCCTGGAAACGGCTGGCCGCCAgg                                  >  3:140375/1‑136 (MQ=255)
                                                                                                        acaacaCCAACATCCTTCCCATCGAGGACGACCCGGTGCTTGGTGCCTATCTGCAGGAAGAGCTGCAGCGTGGCGGCTGCCAGGTCACCTGGTGCCGCAATGGCCTGGAGGGCCTGGAAACGGCTGGCCGCCAGGc                                 <  1:374386/136‑1 (MQ=255)
                                                                                                            cacCAACATCCTTGCCATCGAGGACGACCCGGTGCTTGGTGCCTATCTGCAGGAAGAGCTGCAGCGTGGCGGCTGCCAGGTCACCTGGTGCCGCAATGGCCTGGAGGGCCTGGAAACGGCTGGCCGCCAGGCTTTc                             <  3:9872/136‑1 (MQ=255)
                                                                                                             acCAACATCGTTGCCTTCCAGGACGACCCGGTGCTTGGTGCCTATCTGCAGGAAGAGCTGCAGCGTGGCGGCTGCCAGGTCACCTGGTGCCGCAATGGCCTGGAGGGCCTGGAAACGGCTGGCCGCCAGGCTTTCg                            <  2:18668/136‑1 (MQ=255)
                                                                                                             acCAACATCCTTGCCATCGAGGACGACCCGGTGCTTGGTGCCTATCTGCAGGAAGAGCTGCAGCGTGGCGGCTGCCAGGTCACCTGGTGCCGCAATGGCCTGGAGGGCCTGGAAACGGCTGGCCGCCAGGCTTTCg                            <  4:216599/136‑1 (MQ=255)
                                                                                                                aaCCTCCTTCCCATGGAGGACGACCCGGGGCTTGGTGCCTATCTGCAGGAAGAGCTGCAGCGTGGCGGCTGCCAGGTCACCTGGTGCCGCAATGGCCTGGAGGGCCTGGAAACGGCTGGCCGCCAGGCTTTCGAtg                         <  6:45959/136‑1 (MQ=255)
                                                                                                                aaCATCCTTGCCATCGAGGACGACCCGGTGCTTGGTGCCTATCTGCAGGAAGAGCTGCAGCGTGGCGGCTGCCAGGTCACCTGGTGCCGCAATGGCCTGGAGGGCCTGGAAACGGCTGGCCGCCAGGCTTTCGAtg                         >  4:32597/1‑136 (MQ=255)
                                                                                                                aaCATCCTTGCCATCGAGGACGACCCGGTGCTTGGTGCCTATCTGCAGGAAGAGCTGCAGCGTGGCGGCTGCCAGGTCACCTGGTGCCGCAATGGCCTGGAGGGCCTGGAAACGGCTGGCCGCCAGGCTTTCGAtg                         <  5:201373/136‑1 (MQ=255)
                                                                                                                  cATCCTTGCCATCGAGGACGATCCGGTGCTTGGTGCCTATCTGCAGGAAGAGCTGCAGCGTGGCGGCTGCCAGGTCACCTGGTGCCGCAATGGCCTGGAGGGCCTGGAAACGGCTGGCCGCCAGGCTTTCGAtgtg                       >  4:119173/1‑136 (MQ=255)
                                                                                                                  cATCCTTGCCATCGAGGACGATCCGGTGCTTGGTGCCTATCTGCAGGAAGAGCTGCAGCGTGGCGGCTGCCAGGTCACCTGGTGCCGCAATGGCCTGGAGGGCCTGGAAACGGCTGGCCGCCAGGCTTTCGAtgtg                       >  4:113397/1‑136 (MQ=255)
                                                                                                                  cATCCTTGCCATCGAGGACGACCCGGTGCTTGGTGCCTATCTGCAGGAAGAGCTGCAGCGTGGCGGCTGCCAGGTCACCTGGTGCCGCAATGGCCTGGAGGGCCTGGAAACGGCTGGCCGCCAGGCTTTCGAtgtg                       <  3:349370/136‑1 (MQ=255)
                                                                                                                  cATCCTTGCCATCGAGGACGACCCGGTGCTTGGTGCCTATCTGCAGGAAGAGCTGCAGCGTGGCGGCTGCCAGGTCACCTGGTGCCGCAATGGCCTGGAGGGCCTGGAAACGGCTGGCCGCCAGGCTTTCGAtgtg                       >  8:272134/1‑136 (MQ=255)
                                                                                                                    tCCTTGCCATCGAGGACGATCCGGTGCTTGGTGCCTATCTGCAGGAAGAGCTGCAGCGTGGCGGCTGCCAGGTCACCTGGTGCCGCAATGGCCTGGAGGGCCTGGAAACGGCTGGCCGCCAGGCTTTCGATgtggt                     <  3:119173/136‑1 (MQ=255)
                                                                                                                    tCCTTGCCATCGAGGACGATCCGGTGCTTGGTGCCTATCTGCAGGAAGAGCTGCAGCGTGGCGGCTGCCAGGTCACCTGGTGCCGCAATGGCCTGGAGGGCCTGGAAACGGCTGGCCGCCAGGCTTTCGATgtggt                     <  3:113397/136‑1 (MQ=255)
                                                                                                                    tCCTTGCCATCGAGGACGACCCGGTGCTTGGTGCCTATCTGCAGGAAGAGCTGCAGCGTGGCGGCTGCCAGGTCACCTGGTGCCGCAATGGCCTGGAGGGCCTGGAAACGGCTGGCCGCCAGGCTTTCGATgtggt                     <  3:388186/136‑1 (MQ=255)
                                                                                                                         gCCATCGAGGACGACCCGGTGCTTGGTGCCTATCTGCAGGAAGAGCTGCAGCGTGGCGGCTGCCAGGTCACCTGGTGCCGCAATGGCCTGGAGGGCCTGGAAACGGCTGGCCGCCAGGCTTTCGATGTGGTGCt                  <  8:11921/134‑1 (MQ=255)
                                                                                                                         gCCATCGAGGACGACCCGGTGCTTGGTGCCTATCTGCAGGAAGAGCTGCAGCGTGGCGGCTGCCAGGTCACCTGGTGCCGCAATGGCCTGGAGGGCCTGGAAACGGCTGGCCGCCAGGCTTTCGATGTGGTGCt                  <  8:209323/134‑1 (MQ=255)
                                                                                                                           cATCGAGGACGACCCGGTGCTTGGTGCCTATCTGCAGGAAGAGCTGCAGCGTGGCGGCTGCCAGGTCACCTGGTGCCGCAATGGCCTGGAGGGCCTGGAAACGGCTGGCCGCCAGGCTTTCGATGTGGTGCTGATg              >  3:100686/1‑136 (MQ=255)
                                                                                                                            aTCGAGGACGACCCGGTGCTTGGTGCCTATCTGCAGGAAGAGCTGCAGCGTGGCGGCTGCCAGGTCACCTGGTGCCGCAATGGCCTGGAGGGCCTGGAAACGGCTGGCCGCCAGGCTTTCGATGTGGTGCTGATg              >  8:74051/1‑135 (MQ=255)
                                                                                                                               gAGGACGACCCGGTGCTTGGTGCCTATCTGCAGGAAGAGCTGCAGCGTGGCGGCTGCCAGGTCACCTGGTGCCGCAATGGCCTGGAGGGCCTGGAAACGGCTGGCCGCCAGGCTTTCGATGTGGTGCTGATGGACa          <  2:343123/136‑1 (MQ=255)
                                                                                                                                aGGACGACCCGGTGCTTGGTGCCTATCTGCAGTAAGAGCTGCAGCGTGGCCGCTGCCAGGTCACCTGGTGCCGCAATGGCCTGGAGGGCCTGGAAACGGCTCGCCGCCAGGCTTTCGATGTGGTGCTGATGGACAt         >  6:339346/1‑136 (MQ=255)
                                                                                                                                 ggACGACCCGGTGCTTGGTGCCTATCTGCAGGAAGAGCTGCAGCGTGGCGGCTGCCAGGTCACCTGGTGCCGCAATGGCCTGGAGGGCCTGGAAACGGCTGGCCGCCAGGCTTTCGATGTGGTGCTGATGGACATc        <  8:52747/136‑1 (MQ=255)
                                                                                                                                      acCCGGTGCTTGGTGCCTATCTGCAGGAAGAGCTGCAGCGTGGCGGCTGCCAGGTCACCTGGTGCCGCAATGGCCTGGAGGGCCTGGAAACGGCTGGCCGCCAGGCTTTCGATGTGGTGCTGATGGACATCctgct   <  8:72808/136‑1 (MQ=255)
                                                                                                                                       cccGGTGCTTGGTGCCTATCTGCAGGAAGAGCTGCAGCGTGGCGGCTGCCAGGTCACCTGGTGCCGCAATGGCCTGGAGGGCCTGGAAACGGCTGGCCGCCAGGCTTTCGATGTGGTGCTGATGGACATCctgctg  >  8:183985/1‑136 (MQ=255)
                                                                                                                                       cccGGTGCTTGGTGCCTATCTGCAGGAAGAGCTGCAGCGTGGCGGCTGCCAGGTCACCTGGTGCCGCAATGGCCTGGAGGGCCTGGAAACGGCTGGCCGCCAGGCTTTCGATGTGGTGCTGATGGACATCctgctg  >  7:144899/1‑136 (MQ=255)
                                                                                                                                       |                                                                                                                                       
TGCCGAGGCATTGGTCGCAGTGCGGTTGCCTGGCAGGTGGCGAATGCTCAATAATCACGCAATTGAGAATGCATGGCAGTTGCCAATGAATCCTGTATCTATTTACAACACCAACATCCTTGCCATCGAGGACGACCCGGTGCTTGGTGCCTATCTGCAGGAAGAGCTGCAGCGTGGCGGCTGCCAGGTCACCTGGTGCCGCAATGGCCTGGAGGGCCTGGAAACGGCTGGCCGCCAGGCTTTCGATGTGGTGCTGATGGACATCCTGCTG  >  ACB143/1837958‑1838228

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑