BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB143	2,106,773	T→G	15.9%	E190D (GAA→GAC)	CJ019_02004 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB143	2,106,773	0	T	G	15.9%	252.6 / 9.9	125	E190D (GAA→GAC)	CJ019_02004	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (37/65); new base G (21/2); total (58/67)
Fisher's exact test for biased strand distribution p-value = 1.10e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 125 total aligned reads displayed.

CATGGGTTGGCAGCACGATCGATGTGACCCAGTCCGTGTTGATCAGGTTCATCGCATAAAGGGCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCCAGCAGGATCTCCTCACGCTCGTCGCTGATCGAGCTGAGCATCATGTAAGACGAGTTGAACGGTTGGTACTGGAGGTCGATCCTGTGCCCCGTGGCCGCGTAGATCTCCACCGGCAGGGCGAT  >  ACB143/2106646‑2106904

cATGGGTTGGCAGCACGATCGATGTGACCCAGTCCGTGTTGATCAGGTTCATCGCATAAAGGGCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTc                                                                                                                             >  7:117997/1‑136 (MQ=255)

 aTGGGTTGGCAGCACGATCGATGTGACCCAGTCCGTGTTGATCAGGTTCATCGCATAAAGGGCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGGTGGAtttg                                                                                                                             >  8:258195/1‑134 (MQ=255)

    ggTTGGCAGCACGATCGATGTGACCCAGTCCGTGTTGATCAGGTTCATCGCATAAAGGGCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCcag                                                                                                                          <  8:185074/135‑1 (MQ=255)

      ttGGCAGCACGATCGATGTGACCCAGTCCGTGTTGATCAGGTTCATCGCATAAAGGGCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCcagcag                                                                                                                       <  2:225930/136‑1 (MQ=255)

      ttGGCAGCACGATCGATGTGACCCAGTCCGTGTTGATCAGGTTCATCGCATAAAGGGCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCcagcag                                                                                                                       <  8:152567/136‑1 (MQ=255)

      ttGGCAGCACGATCGATGTGACCCAGTCCGTGTTGATCAGGTTCATCGCATAAAGGGCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCcagcag                                                                                                                       <  7:134992/136‑1 (MQ=255)

      ttGGCAGCACGATCGATGTGACCCAGTCCGTGTTGATCAGGTTCATCGCATAAAGGGCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGGTGGATTTGcagcag                                                                                                                       >  3:233098/1‑136 (MQ=255)

           agcaCGATCGATGTGACCCAGTCCGTGTTGATCAGGTTCATCGCATAAAGGGCAACGGTCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCCAGCAGGAtc                                                                                                                   >  1:115040/1‑135 (MQ=255)

           agcaCGATCGATGTGACCCAGTCCGTGTTGATCAGGTTCATCGCATAAAGGGCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTGGATTTAAAGCAGGAtct                                                                                                                  >  8:32435/1‑136 (MQ=255)

           agcaCGATCGATGTGACCCAGTCCGTGTTGATCAGGTTCATCGCATAAAGGGCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCCAGGAGGAtct                                                                                                                  >  6:170641/1‑136 (MQ=255)

           agcaCGATCGATGTGACCCAGTCCGTGTTGATCAGGTTCATCGCATAAAGGGCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCCAGCAGGAtc                                                                                                                   >  4:181910/1‑135 (MQ=255)

           agcaCGATCGATGTGACCCAGTCCGTGTTGATCAGGTTCATCGCATAAAGGGCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCCAGCACGAtc                                                                                                                   >  3:364954/1‑135 (MQ=255)

           agcaCGATCGATGTGACCCAGTCCGTGTTGATCAGGTTCATCGCATAAAGGGCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTACAGCAGGAtct                                                                                                                  >  6:297438/1‑136 (MQ=255)

           agcaCGATCGATGTGACCCAGTCCGTGTTGATCAGGTTCATCGCATAAAGGGCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTACAGCAGGAtc                                                                                                                   >  1:71271/1‑135 (MQ=255)

           agcaCGATCGATGTGACCCAGTCCGTGTTGATCAGGTTCATCGCATAAAGGGCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTGGGATTTACAGCAGGAtct                                                                                                                  >  2:350060/1‑136 (MQ=255)

           agcaCGATCGATGTGACCCAGTCCGTGTTGATCAGGTTCATCGCATAAAGGGCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGGTGGTTTTCCAGCAGGctct                                                                                                                  >  5:235286/1‑136 (MQ=255)

           agcaCGATCGATGTGACCCAGTCCGTGTTGATCAGGTTCATCGCATAAAGGGCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGGTCGATTTGCAGCAGGGtct                                                                                                                  >  7:216799/1‑136 (MQ=255)

             caCGATCGATGTGACCCAGTCCGTGTTGATCAGGTTCATCGCATAAAGGGAAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCCAGCAGGATctcc                                                                                                                <  6:135918/136‑1 (MQ=255)

                     atGTGACCCAGTCCGTGTTGATCAGGTTCATCGCATAAAGGGCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCCAGCAGGATCTCCTACCGCtc                                                                                                        >  6:140500/1‑136 (MQ=255)

                     atGTGACCCAGTCCGTGTTGATCAGGTTCATCGCATAAAGGGCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTAGATTTACAGCAGGATCTCCTCACGc                                                                                                          >  6:146906/1‑134 (MQ=255)

                          aCCCAGTCCGTGTTGATCAGGTTCATCGCATAAAGGGCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTGGATTTACAGCAGGATCTCCTAAAGCGCGgcgc                                                                                                   >  8:208218/1‑136 (MQ=255)

                            ccAGTCCGTGTTGATCAGGTTCATCGCATAAAGGGCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCCAGCAGGATCTCCTCACGCTCGTCGCTg                                                                                                 <  2:27668/136‑1 (MQ=255)

                            ccAGTCCGTGTTGATCAGGTTCATCGCATAAAGGGCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCCAGCAGGATCTCCTCACGCTCGTCGCTg                                                                                                 <  4:302731/136‑1 (MQ=255)

                             cAGTCCGTGTTGATCAGGTTCATCGCATAAAGGGCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCCAGCAGGATCTCCTCACGCTCGTCGCTGa                                                                                                >  1:11461/1‑136 (MQ=255)

                                tCCGTGTTGATCAGGTTCATCGCATAAAGGGCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCCAGCAGGATCTCCTCACGCTCGTCGCTGATCg                                                                                             <  5:161373/136‑1 (MQ=255)

                                tCCGTGTTGATCAGGTTCATCGCATAAAGGGCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCCAGCAGGATCTCCTCACGCCCGTCGCTGATc                                                                                              <  8:336917/135‑1 (MQ=255)

                                   gtgtTGATCAGGTTCATCGCATAAAGGGCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTGGATTTTAACCAGGATCACCTCACCCACGTCGCTGAACGAGc                                                                                          >  4:217143/1‑136 (MQ=255)

                                   gtgtTGATCAGGTTCATCGCATAAAGGGCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCCAGCAGGATCTCCTAACGCTCGTAGGTGATagagg                                                                                          >  2:13192/1‑135 (MQ=255)

                                   gtgtTGATCAGGTTCATCGCATAAAGGGCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTACAGCAGGATCTACTAACGCTCGTCGCTGATCGAGc                                                                                          >  8:283118/1‑136 (MQ=255)

                                   gtgtTGATCAGGTTCATCGCATAAAGGGCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGATCGCGCAATTCGATACGGCTATCGTCGCGGTAGTATTGCAGGCGGATCGCCACACCCTCGTCGCCGGTCGAGc                                                                                          >  3:287127/1‑136 (MQ=255)

                                      ttGATCAGGTTCATCGCATAAAGGGCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCCAGCAGGATCTCCTCACGCTCGTCGCTGATCGAGCTGa                                                                                       <  1:67371/136‑1 (MQ=255)

                                       tGATCAGGTTCATCGCATAAAGGGCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCCAGCAGGATCTCCTCACGCTCGTCGCTGATCGAGCTGAg                                                                                      >  2:314592/1‑136 (MQ=255)

                                       tGATCAGGTTCATCGCATAAAGGGCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCAAGCAGGATCTCCACACGCTCGTCGCTGATCGCGCTGAg                                                                                      >  1:241818/1‑136 (MQ=255)

                                       tGATCAGGTTCATCGCATAAAGGGCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTACACCAGGATATCCACCCCCACGTCCCTGATCGAGCTGAg                                                                                      >  7:109995/1‑136 (MQ=255)

                                       tGATCAGGTTCATCGCATAAAGGGCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGGTGGATTTGCAACAGGATCACCACACCCACGTCGCTGATCGAGCGGAg                                                                                      >  4:31205/1‑136 (MQ=255)

                                       tGATCAGGTTCATCGCATAAAGGGCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGGTCGATTTCCAGCAGGATCTCCTCAAGCTCGGCGCAGATCGAGCagag                                                                                      >  1:384006/1‑136 (MQ=255)

                                         aTCAGGTTCATCGCATAAAGGGCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGGTGGATTTACAGCAGGATATACAAACCCTCGTCGCGGATAGACCTGAGc                                                                                     >  2:346506/1‑135 (MQ=255)

                                           cAGGTTCATCGCATAAAGGGCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTACAGCAGGATCTCCTCACCCTCGTCGCTGATCGAGCTGAGaat                                                                                   >  6:325732/1‑135 (MQ=255)

                                            aGGTTCATCGCATAAAGGGCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGGTGGATTTCCACCAGGATCTCCTCACGCGCGTCGCGGATAGAACTGAGcatca                                                                                 >  6:107674/1‑136 (MQ=255)

                                               ttCATCGCATAAAGGGCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCCAGCAGGATCTCCTCACGCTCGTCGCTGATCGAGCTGAGCATCATGt                                                                              <  8:309617/136‑1 (MQ=255)

                                                 cATCGCATAAAGTGCTACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTAGAGCAATTCGATGCGGCTATCGTCTCGTTCGATTTCCAGCAGGATCTACTCACGCTCGTCGCTGATCGAGCTGAGCATCATGTaa                                                                            <  2:169581/136‑1 (MQ=255)

                                                 cATCGCATAAAGGGCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTGCATTTACAGCAGGGAATACACAAGCACCTCGCTGATCGACCAGAGCATAAAGTa                                                                             >  8:186697/1‑135 (MQ=255)

                                                 cATCGCATAAAGGGCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTACAGCACGATCTACTCACGCTCGTCGCGGATCGAGCTGAGCATCATGTaa                                                                            >  4:339246/1‑136 (MQ=255)

                                                 cATCGCATAAAGGGCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGGTAGATTTACAGCAGGATCTCCTCACGCGCGTCGCTGATAGAGCTGAGCATCATGt                                                                              >  7:203575/1‑134 (MQ=255)

                                                 cATCGCATAAAGGGCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGGACGATTTACACCAGGAAAACAACACCCACGTCGCTGATCCAGCGGAGAAACATGTa                                                                             >  6:192622/1‑135 (MQ=255)

                                                       aTAAAGGGCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCCAGCAGGGTCTCCTCACGCTCGTCGCTGATCGAGCTGAGCATCATGTAAGACGAg                                                                      >  6:378711/1‑136 (MQ=255)

                                                       aTAAAGGGCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCCAGCAGGATCTCCTCACGCTCGTCGCTGATCGAGCTGAGCATCATGTAAGACGAg                                                                      <  6:174167/136‑1 (MQ=255)

                                                        tataGGTCATCGGGCTGGCCGTTGTCGCCCTGATTAGGGCGGTCTCTCTATTCGATGCGGCTATCGTCGAGTTCGATTTCCAGCAGTATCTCCTCACGCTCGTCGCTGATCGAGCTGAGCATCATGTAATACGAGt                                                                     <  8:226901/133‑1 (MQ=255)

                                                        tAAAGGGCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCCAGCAGGATCTCCTCACGCTCGTCGCTGATCGAGCTGAGCATCATGTAAGACGAGt                                                                     <  5:169079/136‑1 (MQ=255)

                                                          aaGGGCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCCAGCAGGATCTCCTCACGCTCGTCGCTGATCGAGCTGAGCATCATGTAAGACGAGtt                                                                    <  4:372792/135‑1 (MQ=255)

                                                           aGGGCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTACAGCAGGATCTCCTCACGCTCGTCGCTGATCGAGCTGAGCATCATGTAAGACGAGTTg                                                                   >  1:95665/1‑135 (MQ=255)

                                                            gggCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCCAGCAGGATCTCCTCACGCTCGTCGCTGATCGAGCTGAGCATCATGTAAGACGAGTTGaa                                                                 <  4:89377/136‑1 (MQ=255)

                                                             ggCCACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCCAGCAGGATCTCCTCACGCTCGTCGCTGATCGAGCTGAGCATCATGTAAGACGAGTTGAAc                                                                <  4:250382/136‑1 (MQ=255)

                                                             ggCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCCAGCAGGATCTCCTCACGCTCGTCGCTGATCGAGCTGAGCATCATGTAAGACGAGTTGAAc                                                                <  5:298146/136‑1 (MQ=255)

                                                             ggCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCCAGCAGGATCTCCTCACGCTCGTCGCTGATCGAGCTGAGCATCATGTAAGACGAGTTGAAc                                                                <  1:111928/136‑1 (MQ=255)

                                                             ggCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCCAGCAGGATCTCCTCACGCTCGTCGCTGATCGAGCTGAGCATCATGTAAGACGAGTTGAAc                                                                <  3:193927/136‑1 (MQ=255)

                                                             ggCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCCAGCAGGATCTCCTCACGCTCGTCGCTGATCGAGCTGAGCATCATGTAAGACGAGTTGAAc                                                                <  3:162549/136‑1 (MQ=255)

                                                             ggCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGAGGCGGCTATCGTCGCGGTCGATTTCCAGCAGGATCTCCTCACGCTCGTCGCTGATCGAGCTGAGCATCATGTAAGACGAGTTGAAc                                                                <  1:253726/136‑1 (MQ=255)

                                                             ggCAACGGGCTGGCCGGTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCCAGCAGGATCTCCTCACGCTCGTCGCTGATCGAGCTGAGCATCATGTAAGACGAGTTGAAc                                                                <  2:371819/136‑1 (MQ=255)

                                                             ggCAAAGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCCAGCAGGATCTCCTCACGCTCGTCGCTGATCGAGCTGAGCATCATGTAAGACGAGTTGAAc                                                                <  5:24390/136‑1 (MQ=255)

                                                             agacaggggCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCCAGCAGGATCTCCTCACGCTCGTCGCTGATCGAGCTGAGCATCATGTAAGACGAGTTGAAc                                                                <  1:393828/130‑1 (MQ=255)

                                                               cAACGGGCTGGCCGTTGTCGCCCTTATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCCAGCAGGATCTCCTCACGCTCGTCGCTGATCGAGCTGAGCATCATGTAAGACGAGTTGAACgg                                                              <  5:374544/136‑1 (MQ=255)

                                                                aaCGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCCAGCAGGATCTCCTCACGCTCGTCGCTGATCGAGCTGAGCATCATGTAAGACGAGTTGAACGGt                                                             >  8:50245/1‑136 (MQ=255)

                                                                 aCGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTAGCGTCGCGGTCGATTTACACAAGGGGGTCCTCACGCGCGGCCCTGTTCGAGGTGAGCAGCATGTAAGCCGCGTTGAACGCtt                                                            >  2:81729/1‑136 (MQ=255)

                                                                    ggCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTGGATTTACAGCAGGATCTCCTAACCCTCGTCGCTGATCGAGCTGAGCATCATGTAAGACGAGTTGAACGGTTGGt                                                         >  6:293105/1‑136 (MQ=255)

                                                                     gCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCCAGCAGGATCTCCTCACGCTCGTCGCTGATCGAGCTGAGCATCATGTAAGACGAGTTGAACGGTTGGTa                                                        <  1:229242/136‑1 (MQ=255)

                                                                     gCTGGCAGTTGTCGCACTGATAAGGGAGCTCTCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCCAGCAGGATCTCCTAACTCTCGTCGCTGATCGAGCTGAGCATCATGTAAGAAGAGTTGAACGTTTGGTa                                                        <  3:213761/136‑1 (MQ=255)

                                                                          ccGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCCAGCAGGATCTCCTCACGCTCGTCGCTGATCGAGCTGAGCATCATGTAAGACGAGTTGAACGGTTGGTACTGGa                                                   <  5:126970/136‑1 (MQ=255)

                                                                          ccGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCCAGCAGGATCTCCTCACGCTCGTCGCTGATCGAGCTGAGCATCATGTAAGACGAGTTGAACGGTTGGTACTGGa                                                   <  8:376626/136‑1 (MQ=255)

                                                                          ccGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCCAGCAGGATCTCCTCACGCTCGTCGCTGATCGAGCTGAGCATCATGTAAGACGAGTTGAACGGTTGGTACTGGa                                                   <  8:145175/136‑1 (MQ=255)

                                                                          ccGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCCAGCAGGATCTCCTCACGCTCGTCGCTGATCGAGCTGAGCATCATGTAAGACGAGTTGAACGGTTGGTACTGGa                                                   <  7:186697/136‑1 (MQ=255)

                                                                          ccGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCCAGCAGGATCTCCTCACGCTCGTCGCTGATCGAGCTGAGCATCATGTAAGACGAGTTGAACGGTTGGTACTGGa                                                   <  4:131546/136‑1 (MQ=255)

                                                                          ccGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCCAGCAGGATCTCCTCACGCTCGTCGCTGATCGAGCTGAGCATCATGTAAGACGAGTTGAACGGTTGGTACTGGa                                                   <  5:278056/136‑1 (MQ=255)

                                                                          ccGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGGTCGATTTACAGCACGATCTCCAAACCCTCGGCGCTGATCGAGCTGAGCAACATGTAAGACGAGTTGAACCGTTGGTAATg                                                     >  3:60218/1‑134 (MQ=255)

                                                                            gTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTGGATTTCCAGCAGGAGCTCCTCACGCGCGGCGCTGATCGAGCGGAGCCTCATGTAAGGCGAGGTGCACGGGTGGTTCTGGAgg                                                 >  7:109594/1‑136 (MQ=255)

                                                                            gTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCCAGCAGGATCTCCTCACGCTCGTCGCTGATCGAGCTGAGCATCATGTAAGACGAGTTGAACGGTTGGTACTGGAgg                                                 <  8:235757/136‑1 (MQ=255)

                                                                            gTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCCAGCAGGATCTCCTCACGCTCGTCGCTGATCGAGCTGAGCATCATGTAAGACGAGTTGAACGGTTGGTACTGGAgg                                                 <  6:64304/136‑1 (MQ=255)

                                                                            gTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCCAGCAGGATCTCCTCACGCTCGTCGCTGATCGAGCTGAGCATCATGTAAGACGAGTTGAACGGTTGGTACTGGAgg                                                 <  6:368352/136‑1 (MQ=255)

                                                                                 cGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGGTGGATTTGCAGCAGGCTCTCCTCACGCGCGGCGCGGAGAGAGCTGAGCATAATGTTATACGAGTTGAACGGTTGGGTCTGGAgctgtgg                                            >  2:393828/1‑130 (MQ=255)

                                                                                  gCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTGGATTTACAGCCGGATCTCCTCACGCTCGTCGGGGATCGAGCGGAGCATCATGTTAGAAGAGTTGCACGGTTGGGAGTGGTGGTGGAta                                           >  4:163027/1‑135 (MQ=255)

                                                                                  gCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCCAGCAGGATCTCCTCACGCTCGTCGCTGATCGAGCTGAGCATCATGTAAGACGAGTTGAACGGTTGGTACTGGAGGTCGATc                                           <  1:74387/136‑1 (MQ=255)

                                                                                    ccTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCCAGCAGGATCTCCTCACGCTCGTCGCTGATCGAGCTGAGCATCATGTAAGACGAGTTGAACGGTTGGTACTGGAGGTCGATCCt                                         <  2:115040/136‑1 (MQ=255)

                                                                                            gggCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCCAGGAGGATCTCCTCACGCTCGTCGCTGATCGAGCTGAGCATCATGTAAGACGAGTGGAACGGTTGGTACTGGAGGTCGATCCTGTGCCCCg                                 <  3:361295/136‑1 (MQ=255)

                                                                                            gggCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCCAGCAGGATCTCCTCACGCTCGTCGCTGATCGAGCTGAGCATCATGTAAGACGAGTTGAACGGTTGGTACTGGAGGTCGATCCTGTGCCCCg                                 <  8:60051/136‑1 (MQ=255)

                                                                                            gggCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCCAGCAGGATCTCCTCACGCTCGTCGCTGATCGAGCTGAGCATCATGTAAGACGAGTTGAACGGTTGGTACTGGAGGTCGATCCTGTGCCCCg                                 <  3:31205/136‑1 (MQ=255)

                                                                                             ggCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCCAGCAGGATCTCCTCACGCTCGTCGCTGATCGAGCTGAGCATCATGTAAGACGAGTTGAACGGTTGGTACTGGAGGTCGATCCTGTGCCCCg                                 <  8:152992/135‑1 (MQ=255)

                                                                                                 cTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCCAGCAGGATCTCCTCACGCTCGTCGCTGATCGAGCTGAGCATCATGTAAGACGAGTTGAACGGTTGGTACTGGAGGTCGATCCTGTGCCCCGTGGcc                            <  8:179355/136‑1 (MQ=255)

                                                                                                 cTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCCAGCAGGATCTCCTCACGCTCGTCGCTGATCGAGCTGAGCATCATGTAAGACGAGTTGAACGGTTGGTACTGGAGGTCGATCCTGTGCCCCGTGGcc                            <  2:14426/136‑1 (MQ=255)

                                                                                                   cgcgCAATTCGATGCGGCTATCGTCGCGTTCGATTTGCAGCAGGATCTCCTCACGCTCGGCGCTGATCGTGCTGAGCATCATGTAAGACGAGTTGAACGGTTGGTTCTGGAGGTGGATCCTGTTCCCCGTGGCcgc                          >  6:365128/1‑136 (MQ=255)

                                                                                                    gcgcAATTCGATGCGGCTATCGTCGCGTTCGCTTTCCAGCAGGATCTACTCACGCTCGTCGCTGATAGAGCTGAGGATCATTTTAGACGAGGTGAACGGTTGGTTCTGGGGGTCGGTCCTGTGCCCCGTGGCcgcg                         >  8:42277/1‑136 (MQ=255)

                                                                                                     cgcAATTCGATGCGGCTATCGTCGCGTTGGATTTCCAGCAGGATCTCCTCACCCTCGTCGCGGATGGAGCTGAGCATCATGTTAGACGAGTTGAACGGGTGGTTCTGGAGGTCGATCCTGTGCCCCGGGGCcgcgg                        >  6:132362/1‑135 (MQ=255)

                                                                                                     cgcAATTCGATGCGGCTATCGTCGCGTGCGATTTGCAGCACGGTCTACACACGCGCGGCGCGGATCGAGCGGAGGATAAAGTTATACCAATTGAACGGTTGGTAGTTGAGGTCGATCCTGTGCCCCGGGGgcgcc                         >  4:382163/1‑134 (MQ=255)

                                                                                                     cgcAATTCGATGCGGCTATCGTCGCGGTCGATTTCCAGCAGGATATCCTCACCCTCGTCGCGGCTCGAGCGGAGCCTCATGTAAGACGAGTTGAACGGTTGGTACTGGGCGTCGCTCCTGTGCCCCGGGGCCGCGt                        >  2:252209/1‑136 (MQ=255)

                                                                                                     cgcAATTCGATGCGGCTATCGTCGCGGGGGATTTGCAGCAGGCTCTCCGCACGCGCGGCGGTGATAGAGCTGAGCATCATGTAAGACGAGTTGAACGGTTGGTGCTGGCGGTGGATCCTGTGCCCCGTGGCcgcgg                        >  6:346447/1‑135 (MQ=255)

                                                                                                         aTTCGATGCGGCTATCGTCGCGTTCGATTTCCAGCAGGATCTCCTCACGCTCGTCGCTGATCGAGCTGAGCATCATGTAAGACGAGTTGAACGGTTGGTACTGGAGGTCGATCCTGTGCCCCGTGGCCGCGTAGAt                    <  8:244777/136‑1 (MQ=255)

                                                                                                                 cGGCTATCGTCGCGTTCGATTTCCAGCAGGGTCTCCTCACGCTCGTCGCTGATCGAGCTGAGCATCATGTAAGACGAGTTGAACGGTTGGTACTGGAGGTCGATCCTGTGCCCCGTGGCCGCGTAGATCTCCAcc             <  5:378711/135‑1 (MQ=255)

                                                                                                                 cGGCTATCGTCGCGGTCGACTTCCAGCAGGATCTCCTCGCGCGCGTCGCTGCTCGAGCTGAGCTTCATGTAAGACGAGTTGAACGGGTGGTACTGGAGGTCGATCCTGTGCCCCGTGGCCGCGTAGATCTCCACCg            <  2:384315/136‑1 (MQ=255)

                                                                                                                  ggCTAACGTCGCGTTCGATTTCCAGCAGGATCTCCTCACGCTCGTCGCTGATCGAGCTGAGCATCATGTAAGACGACTTGAACGGTTGGTACTGGAGGTCGATCCTGTGCCCCGTGGCCGCGTAGATCTCCACCgg           <  8:144315/136‑1 (MQ=255)

                                                                                                                       tcgtcgCGTTCGATTTCCAGCAGGATCTCCTCACGCTCGTCGCTGGTCGAGCGGAGCATCATGTAAGACGAGTTGAACGGTTGGTTCTGGAGGTCGATCCTGTGCCCCGTGGCCGCGTAGATCTCCCCCGGCCggg      >  7:214138/1‑136 (MQ=255)

                                                                                                                           cgcgTTCGATTTCCAGCAGGATCTCCTCACGCTCGTCGCTGATCGAGCTGAGCATCATGTAAGACGAGTTGAACGGTTGGTACTGGAGGTCGATCCTGTGCCCCGTGGCCGCGTAGATCTCCACCGGCAGGGCGAt  <  3:61712/136‑1 (MQ=255)

CATGGGTTGGCAGCACGATCGATGTGACCCAGTCCGTGTTGATCAGGTTCATCGCATAAAGGGCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCCAGCAGGATCTCCTCACGCTCGTCGCTGATCGAGCTGAGCATCATGTAAGACGAGTTGAACGGTTGGTACTGGAGGTCGATCCTGTGCCCCGTGGCCGCGTAGATCTCCACCGGCAGGGCGAT  >  ACB143/2106646‑2106904

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`