Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 4,747,238 T→G 7.4% S294A (TCG→GCG)  macB → Macrolide export ATP‑binding/permease proteinMacB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1434,747,2380TG 7.4% 666.7 / 2.4 313S294A (TCG→GCG) macBMacrolide export ATP‑binding/permease proteinMacB
Reads supporting (aligned to +/- strand):  ref base T (151/127);  new base G (19/16);  total (170/143)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 320 total aligned reads displayed.

CTGGACCGTGGTGCAACCCAGCACGGTGCCTGGAAAGGTGAACTGCTCGAATCGCTGCAGGCGGCATGGCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGGTGGTGGTGATGTTGGCTGTCGGTGAAGGCAGCAAACGCCAGGTAATGGCACAGATGGCCGCCTTCGGCTCCAACATCCTATACCTCAATGGCAGCCCCCCGACCCTGCGCGAACCGGCTGGGCGCATCACC  >  ACB143/4747103‑4747372
                                                                                                                                       |                                                                                                                                      
cTGGACCGTGGTGCAACCCAGCTCGGTGCCTGGAAAGGTGAACTGCTCGAATCGCTGCAGGCGGCATGGCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGt                                                                                                                                        <  2:373958/136‑1 (MQ=255)
cTGGACCGTGGGTCAACCCAGCACGGTGCCGGGAAAGGTGAACTGCTCGAATCGCTGCAGGCGGCATGGCGAGGGATGTGGATCAACCGCTTCCGCACGGCCCTGACCCTGCGCGGAATCATCATCGGAGTCGCGt                                                                                                                                        <  5:243614/136‑1 (MQ=255)
    aCCGTGGTGCAACCCAGCACGGTGCCTGGAAAGGTGAACTGCTCGAATCGCTGCAGGCGGCATGGCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCggg                                                                                                                                    >  3:36350/1‑135 (MQ=255)
     ccGTGGTGCAACCCAGCACGGTGCCTGGAAAGGTGAACTGCTCGAATCGCTGCAGGCGGCATGGCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCggtg                                                                                                                                   <  1:190007/136‑1 (MQ=255)
       gtggtgCAACCCAGCACGGTGCCTGGAAAGGTGAACTGCTCGAATCGCTGCAGGCGGCATGGCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCggtggt                                                                                                                                 <  7:126883/136‑1 (MQ=255)
        tggtgCAACCCAGCACGGTGCCTGGAAAGGTGAACTGCTCGAATCGCTGCAGGCGGCATGGCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCggtggtg                                                                                                                                <  5:199828/136‑1 (MQ=255)
           tgCAACCCAGCACGGTTCCTGGAAAGGTGAACTGCTCGAATCGCTGCACGCGGCATGGCGAGTGATGTGGATCAACCGCTTCCGCACGGCACAGACCCTGCTCGGAAGCATCAACGGGGTCGCGTCggtggtgggg                                                                                                                             >  6:390267/1‑134 (MQ=255)
           tgCAACCCAGCACGGTGCCTGGAAAGGTGAACTGCTCGAATCGCTGCAGGCGGCATGGCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGgtggtggtg                                                                                                                             >  6:43294/1‑136 (MQ=255)
           tgCAACCCAGCACGGTGCCTGGAAAGGTGAACTGCTCGAATCGCTGCAGGCGGCATGGCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTAGggggtggtg                                                                                                                             >  7:354024/1‑136 (MQ=255)
           tgCAACCCAGCACGGTGCCTGGAAAGGTGAACTGCTCGAATCGCTGCAGGCGGCATGGCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGGCGgtggtggtg                                                                                                                             >  2:340773/1‑136 (MQ=255)
           tgCAACCCAGCACGGTGCCTGGAAAGGTGAACTGCTCGAATCGCTGCAGGCGGCATGGCGAGTGATGTGGATCAACCGCTTCCACACGGCCCTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGgtggtggtg                                                                                                                             >  7:318639/1‑136 (MQ=255)
               aCCCAGCACGGTGCCTGGAAAGGTGAACTGCTCGAATCGCTGCAGGCGGCATGGCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGGTGGTGGTGATGt                                                                                                                         <  2:82409/136‑1 (MQ=255)
                  cAGCACGGTGCCTGGAAAGGTGAACTGCTCGAATCGCTGCAGGCGGCATGGCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGGTGGTGGTGATGTTgg                                                                                                                      >  8:211833/1‑136 (MQ=255)
                        ggTGCCTGGAAAGGTGAACTGCTCGAATCGCTGCAGGCGGCATGGCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGGTGGTGGTGATGTTGGCTGTCg                                                                                                                >  1:238516/1‑136 (MQ=255)
                        ggTGCCTGGAAAGGTGAACTGCTCGAATCGCTGCAGGCGGCATGGCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGGTGGTGGTGATGTTGGCTGTCg                                                                                                                >  5:86073/1‑136 (MQ=255)
                         gTGCCTGGAAAGGTGAACTGCTCGAATCGCTGCAGGCGGCATGGCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGGTGGTGGTGATGTTGGCTGTCgg                                                                                                               <  6:316662/136‑1 (MQ=255)
                             cTGGAAAGGTGAACTGCTCGAATCGCTGCAGTCTGCATGGCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGGTGGTGGTGTTGTTGGGGGTCGGTg                                                                                                             >  1:80333/1‑134 (MQ=255)
                             cTGGAAAGGTGAACTGCTCGAATCGCTGCAGGCGGCATGGGGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGGTGGGGGTGATGTTGGCTGTCGGTg                                                                                                             >  8:371177/1‑134 (MQ=255)
                             cTGGAAAGGTGAACTGCTCGAATCGCTGCAGGCGGCATGGCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGGTGGTGGTGATGTTGGCTGTCGGTg                                                                                                             >  1:21636/1‑134 (MQ=255)
                             cTGGAAAGGTGAACTGCTCGAATCGCTGCAGGCGGCATGGCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGGTGGTGGTGATGTTGGCTGTCGGTg                                                                                                             >  8:269855/1‑134 (MQ=255)
                             cTGGAAAGGTGAACTGCTCGAATCGCTGCAGGCGGCATGGCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGGTGGTGGTGATGTTGGCTGTCGGTg                                                                                                             >  3:278072/1‑134 (MQ=255)
                             cTGGAAAGGTGAACTGCTCGAATCGCTGCAGGCGGCATGGCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGGTGGTGGTGATGTTGGCTGTCGGTg                                                                                                             >  6:295579/1‑134 (MQ=255)
                             cTGGAAAGGTGAACTGCTCGAATCGCTGCAGGCGGCATGGCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGGTGGTGGTGATGTTGGCTGTCGGTg                                                                                                             >  4:380696/1‑134 (MQ=255)
                             cTGGAAAGGTGAACTGCTCGAATCGCTGCAGGCGGCATGGCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGGTGGTGGTGATGTTGGCTGTCGGTGaa                                                                                                           >  2:24639/1‑136 (MQ=255)
                             cTGGAAAGGTGAACTGCTCGAATCGCTGCAGGCGGCATGGCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGGGGGGGGTGCTGTTTGCGTTCgggg                                                                                                             >  2:324870/1‑132 (MQ=255)
                             cTGGAAAGGTGAACAGCTCGAATCGCTGCAGGCGGCATGGAGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGGTGGTGGTGATGTTGGCTGTCGGTg                                                                                                             >  7:45005/1‑134 (MQ=255)
                                      tGAACTGCTCGAATCGCTGCAGGCGGCATGGCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGGTGGTGGTGATGTTGGCTGTCGGTGAAGGCAGCaaa                                                                                                  <  7:185381/136‑1 (MQ=255)
                                         aCTGCTCGAATCGCTGCAGGCGGCATGGCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCggtgg                                                                                                                                  <  8:184853/101‑1 (MQ=255)
                                         aCTGCTCGAATCGCTGCAGGCGGCATGGCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGGCCGTGGTGGTGATGTTGGCTGTCCGTGAAGGCCGCAAACGc                                                                                               >  7:206274/1‑136 (MQ=255)
                                             cTCGAATCGCTGCAGGCGGCATGGCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGGTGGTGGTGATGTTGGCTGTCGGTGAAGGCAGCAAACGCCAgg                                                                                           <  7:149944/136‑1 (MQ=255)
                                                gAATCGCTGCAGGCGGCATGGCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGGTGGTGGTGTTGTTGGGTGTCGGTGAAGGCCGCAAACGCCAGGTaa                                                                                        >  6:236089/1‑136 (MQ=255)
                                                gAATCGCTGCAGGCGGCATGGCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGGGGGTGGTGATGTTGGGTGTCGGTGAAGGCAGCAAACGCCAGGGaa                                                                                        >  3:198023/1‑136 (MQ=255)
                                                   tCGCTGCAGGCGGCATGGCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGGTGGTGGTGATGTTGGCTGTCGGTGAAGGCAGCAAACGCCAGGTAATgg                                                                                     <  7:269855/136‑1 (MQ=255)
                                                     gCTGCAGGCGGCATGGCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGGTGGTGGTGATGTTGGCTGTCGGTGGAGGCAGCAAACGCCAGGTAATGGca                                                                                   >  6:381047/1‑136 (MQ=255)
                                                         cAGGCGGCATGGCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGGTGGTGGTGATGTTGGCTGTCGGTGAAGGCAGCAAACGCCAGGTAATGGCACAGa                                                                               <  5:143030/136‑1 (MQ=255)
                                                         cAGGCGGCATGGCGAGTGATGTGGATCAACCGCTTCAGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGGTGGTGGTGATGTTGGCTGTCGGTGAAGGCAGCAAACGCCAGGTAATGGCACAGa                                                                               <  7:66481/136‑1 (MQ=255)
                                                                    gCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGGTGGTGGTGATGTTGGCTGTCCGTGAAGGCAGCAACCCCCCGGTAATGGCCCAGATGGCCGCCATc                                                                    >  6:169967/1‑136 (MQ=255)
                                                                      gAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGGTGGTGGTGATGTTGGCTGTCGGTGAAGGCAGCAAACGCCAGGTAATGGCACAGATGGCCGCCTTCgg                                                                  <  7:284980/136‑1 (MQ=255)
                                                                         taagggggATCAACCGCTTCCGCACGGCACTGACCCTGGTCGGAATCTTAAGCGGAGTCGCGTCGGTGGGGGTGATGTTGGCTGTCGGTTAAGGCAGCAAACGCCAGGTAATGGCACAGATGGCCGCCTTCGGCTc                                                               <  4:280722/130‑1 (MQ=255)
                                                                         tGATGTGTATCAACCGCTTCCGCACGCCCATTCCCCTGCTCGTAATCATCATCGTAGGCGCGGCGGTGGTGGTGATTTTGGCTGTCGGTGAAGGCAGCAAACGCCAGGTAATGGCACAGATGGCCGCCTTCGGCTc                                                               <  8:341347/136‑1 (MQ=255)
                                                                         tGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGGTGGTGGTGATGTTGGCTGTCGGTGAAGGCAGCAAACGCCAGGTAATGGCACAGATGGCCGCCTTCGGCt                                                                <  3:29525/135‑1 (MQ=255)
                                                                         tGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGGTGGTGGTGATGTTGGCTGTCGGTGAAGGCAGCAAACGCCAGGTAATGGCACAGATGGCCGCCTTCGGCt                                                                <  2:75941/135‑1 (MQ=255)
                                                                         tGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGGTGGTGGTGATGTTGGCTGTCGGTGAAGGCAGCAAACGCCAGGTAATGGCACAGATGGCCGCCTTCGGCt                                                                <  3:322058/135‑1 (MQ=255)
                                                                          gATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGTAATCATCATCGGAGTCGCGTCGGTGGGGGGGATGTTGGCTGTCGGTGAAGGCAGCAAACGCCAGGTAATGGCACAGATGGCCGCCTTCGGCTcc                                                              <  4:1470/136‑1 (MQ=255)
                                                                          gATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGGCGGTGGTGGTTATGTTGGCTGTCGGTGAAGGCAGCAAACGCCAGGTAATGGCACAGATGGCCGCCTTCGGCTc                                                               <  5:43294/135‑1 (MQ=255)
                                                                                gATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGGTGGTGGTGATGTTGGCTGTCGGTGAAGGCAGCAAACGCCAGGTAATGGCACAGATGGCCGCCTTCGGCTCCAACATc                                                        <  6:343882/136‑1 (MQ=255)
                                                                                 aTCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGGTGGTGGTGATGTTGGCTGTCGGTGAAGGCAGCAAACGCCAGGTAATGGCACAGATGGCCGCCTTCGGCt                                                                <  6:81759/127‑1 (MQ=255)
                                                                                 aTCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGGTGGTGGGGATGTTGGCGGTCGGTGAAGGGAGCAAACGCCAGGTAATGGCCCAGATGGCCGCCTTCGGCTCCCACCTcc                                                       >  4:123642/1‑136 (MQ=255)
                                                                                 aTCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGGGGGTGGTGATGTTGGCGGTCGGTGAAGGCAGCAAACCCCCGGTAAAGGCACAGATGGCCGCCATCGGCTCCCACAta                                                        >  8:240106/1‑134 (MQ=255)
                                                                                      ccGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGGTGGTGGTGATGTTGGCTGTCGGTGAAGGCAGCAAACGCCAGGTAATGGCACAGATGGCCGCCTTCGGCTCCAACATCCTATAc                                                  >  2:51579/1‑136 (MQ=255)
                                                                                        gCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGGTGGTGGTGATGTTGGCTGTCGGTGAAGGCAGCAAACGGCAGGTAATGGCACAGATGGCCGCCTTCGGCTCCAACATCCTATAACt                                                >  5:344782/1‑136 (MQ=255)
                                                                                        gCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGGCGGGGGTGGTGTTGTTGGGGGTCGGTGAAGGCAGCAAACGCCAGGGTATGGCACAGATGGCCGCCTGCGGCTCCACCAACCTATAccg                                                >  4:78898/1‑135 (MQ=255)
                                                                                             cGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGGTGGTTGTGATGTTGGCTGTCGGTGAAGGCAGCAAACGCCAGGTAATGGCACAGATGGCCGCCATCGGCTCCAACATCCTATACCTCAATg                                           <  5:309589/136‑1 (MQ=255)
                                                                                             cGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGGTGGTGGTGATGTTGGGTGTCGGTGAAGGCAGCAAACGCCAGGTAATGGCACAGATGGCCGCCTTCGGCTCCAACATCCTATACCTCAATg                                           >  3:93524/1‑136 (MQ=255)
                                                                                              ggaCGGCACTGACCCTGCTCGGAATCATCCTCGGAGTCGCGGCGGTGGTGGTGATGTTGGCTGTCGGTCATGGCAGCAAACGCCAGGTAATGGCACAGATGGCCGCCTTCGGCTCCAACATCCTATACCTCAATgg                                          <  2:157170/134‑1 (MQ=255)
                                                                                              gCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGGTGGTGGTGATGTTGGCTGTCGGTGAAGGCAGCAAACGCCAGGTAATGGCACAGATGGCCGCCTTCGGCTCCAACATCCTATACCTCAATgg                                          <  3:190639/136‑1 (MQ=255)
                                                                                              gCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGGTGGTGGTGATGTTGGCTGTCGGTGAAGGCAGCAAACGCCAGGTAATGGCACAGATGGCCGCCTTCGGCTCCAACATCCTATACCTCAATgg                                          <  5:81447/136‑1 (MQ=255)
                                                                                              gCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGGTGGTGGGTATGTTGGCTGTCGGTGAAGGCAGCAAACGCCAGGTAATGGCACAGATGGCCGCCTTCGGCTCCAACATCCTATACCTCAATgg                                          <  5:290716/136‑1 (MQ=255)
                                                                                              gCACGGCACTGACCCTGCTCAGAATCATCATCGGAGTCGCGTCGGTGGTGGTGATGTTGGCTGTCGGTGAAGGCAGCAAACGCCAGGTAATGGCACAGATGGCCGCCTTCGGCTCCAACATCCTATACCTCAATgg                                          <  4:67670/136‑1 (MQ=255)
                                                                                                            cTGCTCGGAATCATCATCGGAGTCGCGTCGGTGGTGGTGATGTTGGCTGTCGGTGAAGgca                                                                                                       <  7:238504/61‑1 (MQ=255)
                                                                                                            cTGCTCGGAATCATCATCGGAGTCGCGTCGGTGGTGGTGATGTTGGCTGTCGGTGAAGgca                                                                                                       >  8:238504/1‑61 (MQ=255)
                                                                                                                 cGGAATCATCATCGGAGTCGCGTGGGTGGTGGTGTTGTTGGGTGTCGGTGAAGGCCGCAAACGCCAGGTAATGGCACAGATGGCCGCCGTCGGCGCCAACATCCTATACCTCAATGGCCGCCCCCCGACCCAgcgc                       >  1:354455/1‑136 (MQ=255)
                                                                                                                 cGGAATCATCATCGGAGTCGCGTCGGTGGTGGTGATGTTTGGTGTCGGGGAAGGCAGCCAAAGCCAGGTAATGGCACAGATGGCCGCCGTCGGCTCCAACATACTATAACTCAATGGCAGCCCCCCCCCCCAgcgc                       >  2:137917/1‑136 (MQ=255)
                                                                                                                 cGGAATCATCATCGGAGTCGCGTCGGTGGTGGTGATGTTGGGTGTCGGTGAAGGCAGCAAACGCCAGGTAATGGCCCAGATGGCCGCCTTCGGCTCCCACATCCTATACCTCAATGGCAGCCCCCCGCCCCTgcgc                       >  4:342674/1‑136 (MQ=255)
                                                                                                                 cGGAATCATCATCGGAGTCGCGTCGGTGGTGGTGATGTTGGCTGTCGGTGGAGGCAGCAAACGCCAGGTAATGGCACAGATGGCCGCCATCGGCTCCAACATCCTATACCTCAATGGCAGCCCCCCGCCCCGgcgc                       >  4:326498/1‑136 (MQ=255)
                                                                                                                 cGGAATCATCATCGGAGTCGCGTCGGTGGTGGTGATGTTGGCTGTCGGTGAAGGCCGCCAACGCCACGTAATGGCCCCGCTGGCCGCCTTCGGCTCCAGCATCCTAATCCTCAATGGCCGCCCCCCGACCCTgcgc                       >  2:304980/1‑136 (MQ=255)
                                                                                                                 cGGAATCATCATCGGAGTCGCGTCGGTGGTGGTGATGTTGGCTGTCGGTGAAGGCAGCAAACGCCAGGTAATGGCACAGATTGCCGCCTTCGGCTCCAACATCCTATACCTCAATGGCAGCCCCCCGACCCTgcgc                       >  3:210709/1‑136 (MQ=255)
                                                                                                                 cGGAATCATCATCGGAGTCGCGTCGGTGGTGGTGATGTTGGCTGTCGGTGAAGGCAGCAAACGCCAGGTAATGGCACAGATGgccgc                                                                        >  8:299062/1‑87 (MQ=255)
                                                                                                                 cGGAATCATCATCGGAGTCGCGTCGGTGGTGGTGATGTTGGCTGTCGGTGAAGGCAGCAAACGCCAGGTAATGGCACAGATGGCCGCCTTCGGCTCCAACATc                                                        >  3:105966/1‑103 (MQ=255)
                                                                                                                 cGGAATCATCATCGGAGTCGCGTCGGTGGTGGTGATGTTGGCTGTCGGTGAAGGCAGCAAACGCCAGGTAATGGCACAGATGGCCGCCTTCGGCTCCAACATc                                                        <  1:47395/103‑1 (MQ=255)
                                                                                                                 cGGAATCATCATCGGAGTCGCGTCGGTGGTGGTGATGTTGGCTGTCGGTGAAGGCAGCAAACGCCAGGTAATGGCACAGATGGCCGCCTTCGGCTCCAACATCCTATACCTCAATGGCAGCCCCCCGCCCCAgcgc                       >  7:840/1‑136 (MQ=255)
                                                                                                                 cGGAATCATCATCGGAGTCGCGTCGGTGGTGGTGATGTTGGCTGTCGGTGAAGGCAGCAAACGCCAGGTAATGGCACAGATGGCCGCCTTCGGCTCCAACATCCTATACCTCAATGGCAGCCCCCCGACCCTgcgc                       >  5:161957/1‑136 (MQ=255)
                                                                                                                 cGGAATCATCATCGGAGTCGCGTCGGTGGTGGTGATGTTGGCTGTCGGTGAAGGCAGCAAACGCCAGGTAATGGCACAGATGGCCGCCTTCGGCTCCAACATCCTATACCTCAATGGCAGCCCCCCGACCCTgcgc                       >  7:250542/1‑136 (MQ=255)
                                                                                                                 cGGAATCATCATCGGAGTCGCGTCGGTGGTGGTGATGTTGGCTGTCGGTGAAGGCAGCAAACGCCAGGTAATGGCACAGATGGCCGCCTTCGGCTCCAACATCCTATACCTCAATGGCAGCCCCCCGACCCTgcgc                       >  7:295662/1‑136 (MQ=255)
                                                                                                                 cGGAATCATCATCGGAGTCGCGTCGGTGGTGGTGATGTTGGCTGTCGGTGAAGGCAGCAAACGCCAGGTAATGGCACAGATGGCCGCCTTCGGCTCCAACATCCTATACCTCAATGGCAGCCCCCCGACCCTgcgc                       >  2:1817/1‑136 (MQ=255)
                                                                                                                 cGGAATCATCATCGGAGTCGCGTCGGTGGTGGTGATGTTGGCTGTCGGTGAAGGCAGCAAACGCCAGGTAATGGCACAGATGGCCGCCTTCGGCTCCAACATACTATACCTCAATGGCAGCCCCCCGACCCAgcgc                       >  8:38183/1‑136 (MQ=255)
                                                                                                                 cGGAATCATCATCGGAGTCGCGTCGGTGGTGGTGATGTTGGCTGTCGGTGAAGGCAGCAAACGCCAGGTAATGGCACAGATGGCCCCCATCGGCTCCAACATCCTATACCGCAATGGCCGCCCCACGACCCAgcgc                       >  6:195883/1‑136 (MQ=255)
                                                                                                                 cGGAATCATCATCGGAGTCGCGTCGGTGGTGGTGATGTTGGCTGTCGGTGAAGGCAGCAAACCCCAGGTAATGGCACAGATGGCCGCCTTCGGCTCCAACATCCTATACCTCAATGGCAGCCCCCCGACCCTgcgc                       >  3:389227/1‑136 (MQ=255)
                                                                                                                 cGGAATCATCATCGGAGTCGCGTCGGTGGTGGTGATGTTGGCGGTCGGTGTAGGCAGCAAACGCCATGTAATGGGACAGATGGCCGCCGTCGGCTCCAACATCCTATACCACAATGGCAGCCCCCCCCCCCTgcgc                       >  1:143979/1‑136 (MQ=255)
                                                                                                                 cGGAATCATCATCGGAGTCGCGTCGGTGGTGGTGAATTTGGCGGTGGGTGGAGGCAGCAAAAGCCAGCTTATGGCACAGAATGACGCCGACGGCTCCAACATCCTATAACAAAAACGCCGCCCCCCCACCCGgcgc                       >  8:350708/1‑136 (MQ=255)
                                                                                                                 cGGAATCATCATCGGAGTCGCGGGGGTGGTGGTGATGTTGGGTGTGGGTGAAGGCAGCAAACGCCAGGGAATGGCACAGATGGCCGCCTTCGGCTCCAACATCCTATAACTCAAAGGCAGCCCCCCCCCCCAgcgc                       >  1:396449/1‑136 (MQ=255)
                                                                                                                 cGGAATCATCATCGGAGTCGCGGGGGGGGTGGTGTAGTTGGGTGTCGGTGTAGGCCGCAAACCCCCCCTAAAGGCCCCGATGGCCGCCATCGGGAACAACCACCAATACCGCCATGGCAGCCCCCCGCCCCGgcgc                       >  6:274836/1‑136 (MQ=255)
                                                                                                                 cGGAATCATCATCGGAGTCGCGGCGGTGGTGGTGATGTTGGCTGTCGGTGAAGGCAGCAAACGCCAGGTAATTGCACAGATGGCCGCCTTCGGCTGCAACATCCTATACCTCAATGGCCGCCCCCCCCCCCGgcgc                       >  1:309925/1‑136 (MQ=255)
                                                                                                                 cGGAAACATCATCGGAGTCGCGTCGGGGGTGGTGTTGTTGGCTGGGGGTGAAGGCCGCAAACGCCAGGTTATGGGCCAGATGGCCGCCGTCGGCTGCAACATCCGATACCTCAATTGCAGCCCCCCCCCCCAgcgc                       >  3:146173/1‑136 (MQ=255)
                                                                                                                  ggATTCCTCTTCGGAGTGGCGTCGGTGGTGGTGATGTTGGCTGTCTGTGAAGGCAGGATACGCCAGGTAATGGCACAGATGGCCGCCTTCGGCTCCAACATCCTATACCTCAATGGCAGCCCCCCGACCCTgcg                        <  3:376888/134‑1 (MQ=255)
                                                                                                                  ggAATCATCATGGTAGTCGCGGCGGTGGTTGGGATCTTGCTTGTCGTTTAAGGCAGAAAACGCCAGGTAATGGCACAGATGGCCGCCTTCGGCTCCAACATCCTATACCTCAAAGGCAGCCCCCCGACCCTgcgc                       <  4:230156/135‑1 (MQ=255)
                                                                                                                       catcatcGGAGTCGCGTCGGTGGTGGTGATGTTGGCTGTCGGTGAAGGCAGCAAACGCCAGGTAATGGCACAGATGGCCGCCTTCGGCTCCAACATCCTATACCTCAATGGCAGCCCCCCGACCCTGCGCGAACCg                 >  1:55470/1‑136 (MQ=255)
                                                                                                                           atcGGAGTCGCGTCGGTGGTGGTGATGTTGGCTGTCGGTGAAGGCAGCAAACGCCAGGTAATGGCACAGATGGCCGCCTTCGGCTCCAACATCCTATACCTCAAt                                            <  1:289247/105‑1 (MQ=255)
                                                                                                                            tcGGAGTCGCGTCGGTGGTGGTGATGTTGGCTGTCGGTGAAGGCAGCAAACGCCAGGTAATGGCACAGATGGCCGCCTTCGGCTCCAACAACCTATACCTCAATGGCAGCCCCCCGACCCTGCGCGAACCCGCTgg            >  2:284572/1‑136 (MQ=255)
                                                                                                                              ggAGTCGCGTCGGTGGTGGTGTTGTTGGCTGTCGGTGACGGCAGCAAACGCCAGGTAATGGCACAGATGGCCGCCTTCGGCTCCAACATCCTATACCTCAATGGCAGCCCCCCGACCCTGCGCGAACCGGCTGGgc          <  4:189352/136‑1 (MQ=255)
                                                                                                                              ggAGTCGCGTCGGTGGTGGTGATGTTGGCTGTCGGTGAAGGCAGCAAACGCCAGGTAATGGCACAGATGGCCGCCTTCGGCTCCAACATCCTATACCTCAATGGCAGCCCCCCGACCCTGCGCGAACCGGCTGGgc          <  1:332383/136‑1 (MQ=255)
                                                                                                                              ggAGTCGCGTCGGTGGTGGTGATGTTGGCTGTCGGTGAAGGCAGCAAACGCCAGGTAATGGCACAGATGGCCGCCTTCGGCTCCAACATCCTATACCTCAATGGCAGCCCCCCGACCCTGCGCGAACCGGCTGGgc          <  4:210709/136‑1 (MQ=255)
                                                                                                                              ggAGTCGCGGCGGTGGTGGTGATGTTGGCTGTCGGTGAAGGCAGCAAACGCCAGGTAATGGCACAGATGGCCGCCTTCGGCTCCAACATCCTATACCTCAATGGCAGCCCCCCGACCCTGCGCGAACCGGCTGGgc          <  5:279153/136‑1 (MQ=255)
                                                                                                                              ggAGTCGCGGCGGTGGTGGTCATGTTGGCTGTCGGTGAAGGCAGCACACGCCAGGTAATGGCACAGATGGCCGCCTTCGGCTCCAACATCCTATACCTCAATGGCAGCCCCCCGACCCTGCGCGAACCGGCTGGgc          <  1:179921/136‑1 (MQ=255)
                                                                                                                               gAGTCGCGTCGGTGGTGGTGATGTTGGCTGTCGGTGAAGGCAGCAAACGCCAGGTAATGGCACAGATGGCCGCCTTCGGCTCCAACATCCTATACCTCAATGGCAGCCCCCCGACCCTGCGCGAACCGGCTGGgcg         <  3:236237/136‑1 (MQ=255)
                                                                                                                               gAGTCGCGTCGGTGGGGGTGATGTTGGCTGTCTGTGAAGTCAGCAAACGCCAGGTAATGGCACAGATGGCCGCCTTCGGCTCCAACATCCTATACCTCAATGGCAGCCCCCCGACCCTGCGCGAACCGGCTGGgcg         <  1:323025/136‑1 (MQ=255)
                                                                                                                                  tCGCGTCGGTGGTGGTGATGTTGGCTGTCGGTGAAGGCAGCAAACGCCAGGTAATGGCACAGATGGCCGCCTTCGGCTCCAACATCCTATACCTCAATGGCAGCCCCCCGACCCTGCGCGAACCGGCTGGGCGCAt      <  1:95397/136‑1 (MQ=255)
                                                                                                                                   cgcgTGGGCGGTGGTGATGTTGGCTGTCGGTTAAGGCATCAAACGCCAGGTAATGGCACAGATGGCCGCCTTCGGCTCCAACATCCTATACCTCAATGGCAGCCCCCCGACCCTGCGCGAACCGGCTGGGCGCATc     <  8:120122/136‑1 (MQ=255)
                                                                                                                                    gcgTCGGTGGTGGTGATGTTGGCTGTCGGTGAAGGCAGCAAACGCCAGGTAATGGCACAGATGGCCGCCTTCGGCTCCAACATCCTATACCTCAATGGCAGCCCCCCGACCCTGCGCGAACCGGCTGGGCGCATc     <  5:118821/135‑1 (MQ=255)
                                                                                                                                     cgTCGGTGGTGGTGATGTTGGCTGTCGGTGCAGGCAGCAAACGCCACGTAATGGCACAGATGGCCGCCTTCGGCTCCAACATCCTATACCTCAATGGCCGCCCCCCGCCCCTGCGCGCACCGGCTGGGCGGATCAc   >  2:217464/1‑136 (MQ=255)
                                                                                                                                       |                                                                                                                                      
CTGGACCGTGGTGCAACCCAGCACGGTGCCTGGAAAGGTGAACTGCTCGAATCGCTGCAGGCGGCATGGCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGGTGGTGGTGATGTTGGCTGTCGGTGAAGGCAGCAAACGCCAGGTAATGGCACAGATGGCCGCCTTCGGCTCCAACATCCTATACCTCAATGGCAGCCCCCCGACCCTGCGCGAACCGGCTGGGCGCATCACC  >  ACB143/4747103‑4747372

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: