Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 4,761,978 T→G 12.7% intergenic (‑27/+16) lip2 ← / ← grsB Lipase 2/Gramicidin S synthase 2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1434,761,9780TG12.7% 651.4 / 7.7 302intergenic (‑27/+16)lip2/grsBLipase 2/Gramicidin S synthase 2
Reads supporting (aligned to +/- strand):  ref base T (145/106);  new base G (2/49);  total (147/155)
Fisher's exact test for biased strand distribution p-value = 3.60e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 322 total aligned reads displayed.

GGCGGCGCGCCTCGTCCGCCGCCAGGGCATGCATGGGCAGCACCTTGCCGGCGCTGCGTCCGGCCTCCACCAGTTGCAGGTAGGCCGCCAGGTCAGGGTTCAGGGACATCGTTCGATTCTCCAGAGGGGCCGCGCTGGCGGCCCCGAAAGCTCATGAAAGTTCAATGAAGCAGGCGTGCGTGAGGTTGGGTTCGAGCAGCGCTTGTACGTCGTCGAGCAGGGTGGCCTCATGCGGCACCTGGAAGTGCCCACAGGCCAGCAGCCGCGCAGT  >  ACB143/4761843‑4762113
                                                                                                                                       |                                                                                                                                       
ggcggcGCGCCTCGTCCGCCGCCAGGGCATGCATGGGCAGCACCTTGCCGGCGCTGCGTCCGGCCTCCACCAGTTGCAGGTAGGCCGCCAGGTCAGGGTTCAGGGACATCGTTCGATTCTCCAGAGGGGCCGCGCt                                                                                                                                         >  7:178023/1‑136 (MQ=255)
   ggcgCGCCTCGTCCGCCGCCAGGGCATGCATGGGCAGCACCTTGCCGGCGCTGCGTCCGGCCTCCACCAGTTGGAGGTAGGCCGCCAGGTCAGGGTTCAGGGACATCGTTCGATTCTCCAGAGGGGCCGCGCTggc                                                                                                                                      >  1:339624/1‑136 (MQ=255)
   ggcgCGCCTCGTCCGCCGCCAGGGCATGCATGGGCAGCACCTTGCCGGCGCTGCGTCCGGCCTCCACCAGTTGCAGGTAGGCCGCCAGGTCAGGGTTCAGGGACATCGTTCGATTCTCCAGAGGGGCCGCGCTggc                                                                                                                                      >  6:219905/1‑136 (MQ=255)
     cgcgcCTCGTCCGCCGCCAGGGCATGCATGGGCAGCACCTTGCCGGCGCTGCGTCCGGCCTCCACCAGTTGCAGGTAGGCCGCCAGGTCAGGGTTCAGGGACATCGTTCGATTCTCCAGAGGGGCCGCGCTggcgg                                                                                                                                    >  1:316089/1‑136 (MQ=255)
        gcCTCGGCCGCCGCCAGGGCATGCAGGGGCAGCACCTTGCCGGCGCTGCGGCCGGCCTCCACCAGTTGCAGGTAGGCCGCCAGGTCAGGGTTCAGGGACATCGTTCGATTCACCAGCGGGGCCGCGCGGGCGGccc                                                                                                                                 <  5:368096/136‑1 (MQ=255)
        gcCGCGCCCGCCGCCCGGGCATGCATGGGCAGCACCTTGCCGGCGCTGCGTCCGGCCGCCACCAGTTGCAGGAAGGCCGCCAGGTCAGGGTGCCGGGACATCGTTCGATTCTCCCGCGGGGCCGCGCTGGCGGccc                                                                                                                                 <  7:25107/136‑1 (MQ=255)
              tCCGCCGCCAGGGCATGCATGGGCAGCACCTTGCCGGCGCTGCGTCCGGCCTCCACCAGTTGCAGGTAGTCCGCCAGGTCAGGGTTCAGGGACATCGTTCGATTCTCCAGAGGGGCCGCGCTGGGGGCCCCGAAAg                                                                                                                           >  6:376863/1‑136 (MQ=255)
                cgccgcCAGGGCATGCATGGGCAGCACCTTGCCGGCGCTGCGTCCGGCCTCCACCAGTTGCAGGTCGGCCGCCAGGTCAGGGTTCAGGGACATCGTTCGATTCTCCAGAGGGGCCGCGCTGGCGGCCCCGAAAGCt                                                                                                                         <  2:23722/136‑1 (MQ=255)
                  ccgccTGGGCATGCTTGGGCCGAACGTGGCGGGGGCTGCGCCCGGCCTCCACCTGTTGCAGGTCGGCCGCCAGGGCTGGGTGCTGGCACATCGTTCGATTCTCCAGTGGGGCGGCGCTGGGGGCCCCGAAAGCTCa                                                                                                                       <  6:367373/136‑1 (MQ=255)
                          gcattcttGGGCAGCACATGGCCGGCGATGCGTCCGGCCGCAACCTTTTGCTGTTAGGCCGCCTGGTCTGGGTTCAGGGACTTCGTTCGATTCTCCAGAGGGGCCGCGCTTGCGGCCCCGAAAGCTCATGAAAGtt                                                                                                               <  7:63641/136‑1 (MQ=255)
                            aggcatgGGCAGCACCTTGCCGGCGCTGCGTCCGGCCTCCACCAGTTGCCGGTAGGCCGCCAGGTCAGGGTTCAGGGACATCGTTCGATTCTCCCGCGGGGCCGCGCTGGCGGCCCCGAAAGCTCATGAAAGTTCa                                                                                                             <  1:229422/134‑1 (MQ=255)
                                    gctgcaCCTTGCCGGCGCGGCGTCCGGCCTCCACCAGTTGCAGGTAGGCCGCCCGGGCTGGGTTCAGGGACATCGTTCGATTCTCCACAGGGGCCGCGCGGGCGGCCCCGAAAGCTCATGAAAGTTCAATGAAGCa                                                                                                     <  2:77018/133‑1 (MQ=255)
                                      agcaCCTTGCCGGCGCTGCGTCCGGCCTCCACCAGTTGCAGGTAGGCCGCCAGGTCAGGGTTCAGGGACATCGTTCGATTCTCCAGAGGGGCCGCGCTGGCGGCCCCGAAAGCTCATGAAAGTTCAATGAAGCAgg                                                                                                   >  2:307034/1‑136 (MQ=255)
                                       gcaCCTTGCCGGCGCTGCGTCCGGCCTCCACCAGTTGCAGGTAGGCCGCCAGGTCAGGGTTCAGGGACATCGTTCGATTCTCCAGAGGGGCCGCGCTGGCGGCCCCGAAAGCTCATGAAAGTTCAATGAAGCAGgc                                                                                                  >  1:375631/1‑136 (MQ=255)
                                        cacaTTGCCGGCGCTGCGGCCGGCCTCCACCAGTTGCAGGTCGGCCGCCAGGTCAGGGTTCAGGGACATCGTGCGATTCGCCAGAGGGGCCGCGCGGGCGGCCCCGAAAGCTCATGAAAGTTCAATGAAGCAGgcg                                                                                                 <  4:311318/136‑1 (MQ=255)
                                          cgttGCCGGCGCGGCGTCCGGCCTCCACCCGTTGCAGGTAGGCCGCCAGGTCCGGGTTCAGGGACATCGTTCTTTTCCCCAGAGGGGCCGCGCGGGCGGCCCCGAAAGCTCATGAAAGTTCAATGAAGCAGgcgtg                                                                                               <  6:156989/134‑1 (MQ=255)
                                           cTTGCCGGCGCTGCGTCCGGCCTCCACCAGTTGCAGGTAGGCCGCCAGTGCAGGGTTCAGGGACCTCGTTCGATTCTACAGAGGGGCCGCGATGGCGGCCCCGAAAGCTCATGAAACTTCAATGAAGAAGgcgtgc                                                                                              >  6:128436/1‑136 (MQ=255)
                                           cTTGCCGGCGCTGCGTCCGGCCTCCACCAGTTGCAGGTAGGCCGCCAGGTCAGGGTTCAGGGACATCGTTCGATTCTCCAGAGGGGCCGCGCTGGCGGCCCCGAAAGCTCATGAAAGTTCAATGACGCAGgcgtgc                                                                                              >  2:176245/1‑136 (MQ=255)
                                           attGCCGGCGCTGCGTCCGGCCTCCACCAGTTGCAGGTAGGCCGCCCGGTCAGGGTTCAGGGACATCGTTCGATTCTCCAGAGGGGCCGCGCGGGCGGCCCCGAAAGCTCCTTAAAGTTCAATGAAGCAGgcgtgc                                                                                              >  4:119853/2‑136 (MQ=255)
                                             tGCCGGCGCTGCGTCCGGCCTCCACCAGTTGCAGGTAGGCCGCCAGGTCAGGGTTCAGGGACATCGTTCGATTCTCCAGAGGGGCCGCGCTGGCGGCCCCGCAAGCTCATGAAAGTTCAATGAAGCAGgcgtgcgt                                                                                            >  6:7942/1‑136 (MQ=255)
                                                cGGCGCTGCGTCCGGCCTCCTCCAGTTGCAGGTAGGCCGCCAGGTCAGGGTTCAGGGACATCGTTCGATTCTCCAGAGGGGCCGCGCTGGCGGCCCCGAAAGCTCATGAAAGTTCAATGAAGCAGGCGTGCGTGAg                                                                                         >  4:204770/1‑136 (MQ=255)
                                                cGGCGCTGCGTCCGGCCTCCACCAGTTGCAGGTAGGCCGCCAGGTCAGGGTTCAGGGACATCGTTCGATTCTCCAGAGGGGCCGCGCTGGCGGCCCCGAAAGCTCATGAAAGTTCAATGAAGCAGGCGTGCGTGAg                                                                                         >  7:282244/1‑136 (MQ=255)
                                                    gcTGCGGCGGGCCTCCACCTGTGGCAGGTCGGCCGCCAGGGCAGGGTTCAGGGACATCGTTCGTTTCTCCAGCGGGGCCGCGCGGGCGGCCCCGAAAGCTCATGAAAGTTCAATGAAGCAGGCGTGCGTGAGGTTg                                                                                     <  4:94571/136‑1 (MQ=255)
                                                     cTGCGTCCGGCCTCCACCAGTTGCAGGTAGGCCGCCAGGTCAGGGTTCAGGGACATCGTTCGATTCTCCAGAGGGGCCGCGCTGGCGGCCCCGAAAGCTCATGAAAGTTCAATGAAGCAGGCGTGCGTGAGGTTgg                                                                                    >  1:154070/1‑136 (MQ=255)
                                                     cTGCGTCCGGCCTCCACCAGTTGCAGGTAGGCCGCCAGGTCAGGGTTCAGGGACATCGTTCGATTCTCCAGAGGGGCCGCGCTGGCGGCCCCGAAAGCTCATGAAAGTTCAATGAAGCAGGCGTGCGTGAGGTTgg                                                                                    >  3:328492/1‑136 (MQ=255)
                                                     cTGCGTCCGGCCTCCACCAGTTGCAGGTAGGCCGCCAGGTCAGGGTTCAGGGACATCGTTCGATTCTCCAGAGGGGCCGCGCTGGCGGCCCCGAAAGCTCATGAAAGTTCAATGAAGCAGGCGTGCGTGAGGTTgg                                                                                    >  3:166623/1‑136 (MQ=255)
                                                           gcGGCCGCCACCTGGTCCTGGTCGCCGGCCTGGTCTGGTTTCCGGGAAATCGTTGGATTCTCCCGCGGGCCCGCGCGGGGGGCCCCGAAAGCTCATGAAAGTTCAATGAAGCAGGCGTGCGTGAGGTTGGGTTCGa                                                                              <  6:306058/135‑1 (MQ=255)
                                                           ccGGCCTCCACCAGTTGCAGGTAGGCCGCCAGGTCAGGGTTCAGGGACAGCGGTCGATTCTCCAGAGGGGCCGCGCGGGCGGCCCCGAAAGCTCATGCAACATCATAGAAGAAGGCGTGCGTGAGGTTGGGtag                                                                                >  6:119991/1‑132 (MQ=255)
                                                            cGGCCTCCACCCGTTGCAGGTAGGCCGCCAGGTCAGGGTTCAGGGACAGCGTTCTATTCTCCAGAGGGGCCGCGCTGGCGGCCCCGAAAGCTCATGAAAGTTCAATGAAGCAGGCGTGCGTGAGGTTGGGGTCGag                                                                             <  7:302036/136‑1 (MQ=255)
                                                                   caccaGTTGCAGGTAGGCCGCCAGGTCAGGGTTCAGGGACATCGTTCGATTCTCCAGAGGGGCCGCGCTGGCGGCCCCGAAAGCTCATGAAAGTTCAATGAAGCAGGCGTGCGTGAGGTTGGGTTCGAGCAGCGCt                                                                      >  8:234682/1‑136 (MQ=255)
                                                                    accaGTTGCAGGTAGGCCGCCAGGTCTGGGTTCAGGGACATCGTTCGATTCTCCAGCGGGGCCGCGCTGGCGGCCCCGAAAGCTCATGAAAGTTCAATGAAGCAGGCGTGCGTGAGGTTGGGTTCGAGCAGCGCtt                                                                     <  7:129250/136‑1 (MQ=255)
                                                                    accaGTTGCAGGTAGGCCGCCAGGTCAGGGTTCAGGGACATCGTTCGATTCTCCAGAGGGGCCGCGCTGGCGGCCCCGAAAGCTCATGAAAGTTCAATGAAGCAGGCGTGCGTGAGGTTGGGTTCGAGCAGCGCtt                                                                     <  2:277844/136‑1 (MQ=255)
                                                                      caGTTGCAGGTAGGCCGCCAGGTCAGGGTTCAGGGACATCGTTCGATTCTCCAGAGGGGCCGCGCTGGCGGCCCCGAAAGCTCATGAAAGTTCAATGAAGCAGGCGTGCGTGAGGTTGGGTTCGAGCAGCGCTTg                                                                    >  7:559/1‑135 (MQ=255)
                                                                      caGTTGCAGGTAGGCCGCCAGGTCAGGGTTCAGGGACATCGTTCGATTCTCCAGAGGGGCCGCGCTGGCGGCCCCGAAAGCTCATGAAAGTTCAATGAAGCAGGCGTGCGTGAGGTTGGGTTCGAGCAGCGCTTGt                                                                   <  1:244907/136‑1 (MQ=255)
                                                                         ttGCAGTTAGGCCGCCTGGTCAGGGTTCCGGGACAGCGTTCGATTCTCCAGAGGGGCCGCGCTGGCGGCCCCGAAAGCTCATGAAAGTTCAATGAAGCAGGCGTGCGTGAGGTTGGGTTCGAGCAGCGCTTGTAcg                                                                <  5:262554/136‑1 (MQ=255)
                                                                         ttGCAGGTCGGCCGCCAGTTCAGGGGTCTGGGACATCGTTCGATTCTCCAGAGGGGCCGCGCTGGCGGCCCCGAAAGCTCATGAAAGTTCAATGAAGCAGGCGTGCGTGAGGTTGGGTTCGAGCAGCGCTTGTAcg                                                                <  2:122964/136‑1 (MQ=255)
                                                                         tggctggTCGGCCGCCTGGTCCGGGGTCAGCGACATCGTTCGTTTCTCCAGCGGGGCCGCGCTGGCGGCCCCGAAAGCTCATGAAAGTTCAATGAAGCAGGCGTGCGTGAGGTTGGGTTCGAGCAGCGCTTGTAcg                                                                <  4:230260/131‑1 (MQ=255)
                                                                              ggTAGGCCGCCAGGTCAGGGTTCAGGGACATCGTTCGATTCTCCAGAGGGGCCGCGCTGGCGGCCCCGAAAGCTCATGAAAGTTCAATGAAGCAGGCGTGCGTGAGGTTGGGTTCGAGCAGCGCTTGTACgtcgtc                                                           <  1:95080/136‑1 (MQ=255)
                                                                              ggTAGGCCGCCAGGTCAGGGTGCAGGTACATCGTTCGATTCTCCAGCGGGGCCGCGCTGGCGGCCCCGAAAGCTCATGAAAGTTCAATGAAGCAGGCGTGCGTGAGGTTGGGTTCGAGCAGCGCTTGTACgtcgtc                                                           <  7:98440/136‑1 (MQ=255)
                                                                                 aGGCCGCCAGGTCAGGGTTCAGGGACATCGTTCGATTCTCCAGAGGGGCCGCGCTGGCGGCCCCGAAAGCTCATGAAAGTTCAATGAAGCAGGCGTGCGTGAGGTTGGGTTCGAGCCGCGCTTGTACGTCGTCGAg                                                        >  4:371827/1‑136 (MQ=255)
                                                                                 aGGCCGCCAGGTCAGGGTTCAGGGACATCGTTCGATTCTCCAGAGGGGCCGCGCTGGCGGCCCCGAAAGCTCATGAAAGTTCAATGAAGCAGGCGTGCGTGAGGTTGGGTTCGAGCAGCGCTTGTACGTCGTCGAg                                                        >  8:26280/1‑136 (MQ=255)
                                                                                      gccAGGTCAGGGTTCAGGGACATCGTTCGATTCTCCAGAGGGGCCGCGCTGGCGGCCCCGAAAGCTCATGAAAGTTCAATGAAGCAGGCGTGCGTGAGGTTGGGTTCGAGCAGCGCTTGTACGTCGTCGAGCAggg                                                   >  2:287793/1‑136 (MQ=255)
                                                                                        cAGGTCAGGGTTCTGGTACATCGTTCGATTCTCCAGCGGGGCCGCGCTGGCGGCCCCGAAAGCTCATGAAAGTTCAATGAAGCAGGCGTGCGTGAGGTTGGGTTCGAGCAGCGCTTGTACGTCGTCGAGCAGGGTg                                                 <  5:177563/136‑1 (MQ=255)
                                                                                          ggTCAGGGTTCAGGGACATCGTTCGATTCTCCAGAGGGGCCGCGCTGGCGGCCCCGAAAGCTCATGAAAGTTCAATGAAGCAGGCGTGCGTGAGGTTGGGTTCGAGCAGCGCTTGTACGTCGTCGAGCAGGGTGGc                                               >  8:352947/1‑136 (MQ=255)
                                                                                             ccgggTGCAGGTTCATCGTTCGATTCTCCCGCGGGGCCGCGCGGGCGGCCCCGAAAGCTCATGAAAGTTCAATGAAGCAGGCGTGCGTGAGGTTGGGTTCGAGCAGCGCTTGTACGTCGTCGAGCAGGGTGGCCTc                                            <  2:261377/134‑1 (MQ=255)
                                                                                              aGGGTTCAGGGACATCGTTCGATTCTCCAGCGGGGCCGCGCTGGCGGCCCCGAAAGCTCATGAAAGTTCAATGAAGCAGGCGTGCGTGAGGTTGGGTTCGAGCAGCGCTTGTACGTCGTCGAGCAGGGTGGCCTc                                            <  5:148942/135‑1 (MQ=255)
                                                                                               gggTTCAGGGACATCGTTCGATTCTCCAGAGGGGCCGCGCTGGCGGCCCCGAAAGCTCATGAAAGTTCAATGAAGCAGGCGTGCGTGAGGTTGGGTTCGAGCAGCGCTTGTACGTCGTCGAGCAGGGTGGCCTCAt                                          <  8:111787/136‑1 (MQ=255)
                                                                                               gggTCCCGGGACATCGTTCGATTCTCCAGCGGGGCCGCGCGGGCGGCCCCGAAAGCTCATGAAAGTTCAATGAAGCAGGCGTGCGTGAGGTTGGGTTCGAGCAGCGCTTGTACGTCGTCGAGCAGGGTGGCCTCAt                                          <  3:151663/136‑1 (MQ=255)
                                                                                                ggTTCAGGGACATGGTTCGAATCACCAGGGGGGCCGGGCTGGGGGCCCCGAAAGCTCATGAAAGTTCAATGAAGCAGGCGTGCGTGAGGTTGGGTTCGAGCAGCGCTTGTACGTCGTCGAGCAGGGTGGCCTc                                            <  4:166623/133‑1 (MQ=255)
                                                                                                ggTTCAGGGACATCGTTCGATTCTCCAGAGGGGCCGCGCTGGCGGCCCCGAAAGCTCATGAAAGTTCAATGAAGCAGGCGTGCGTGAGGTTGGGTTCGAGCAGCGCTTGTACGTCGTCGAGCAGGGTGGCCTCAt                                          <  7:214363/135‑1 (MQ=255)
                                                                                                        gACATCGTTCGATTCTCCAGAGGGGCCGCGCTGGCGGCCCCGAAAGCTCATGAAAGTTCAATGAAGCAGGCGTGCGTGAGGTTGGGTTCGAGCAGCGCTTGTACGTCGTCGAGCAGGGTGGCCTCATGCGGCACCt                                 >  3:252876/1‑136 (MQ=255)
                                                                                                        gACATCGTTCGATTCTCCAGAGGGGCCGCGCTGGCGGCCCCGAAAGCTCATGAAAGTTCAATGAAGCAGGCGTGCGTGAGGTTGGGTTCGAGCAGCGCTTGTACGTCGTCGAGCAGGGTGGCCTCATGCGGCACCt                                 >  5:47931/1‑136 (MQ=255)
                                                                                                        gACAACGTTAGATTCTCCAGAGGGGCCCCGCTGGCGGCCCCGAAAGCTCATGAAAATTCAAAGAAGCAGGCGTGCGTGAGGTTTGGTTCGAGCACCCCTTGTACCTCGTCGAGCACGGTGGCCTCAAGCAGCAc                                   >  8:72443/1‑134 (MQ=255)
                                                                                                         ccagcGTTCGATGCTCCCGAGGGGCCGCGCTGGCGGCCCCGAAAGCTCATGAAAGTTCAATGAAGCAGGCGTGCGTGAGGTTGGGTTCGAGCAGCGCTTGTACGTCGTCGAGCAGGGTGGCCTCATGCGGCACCTg                                <  6:293321/132‑1 (MQ=255)
                                                                                                               ttCGATTCTCCAGAGGGGCCGCGCTGGCGGCCCCGAAAGCTCATGAAAGTTCAATGAAGCAGGCGTGCGTGAGGTTGGGTTCGAGCAGCGCTTGTACGTCGTCGAGCAGGGTGGCCTCATGCGGCACCTGGAAGTg                          >  3:76257/1‑136 (MQ=255)
                                                                                                               ttCGATTCTCCAGAGGGGCCGCGCTGGCGGCCCCGAAAGCTCATGAAAGTTCAATGAAGCAGGCGTGCGTGAGGTTGGGTTCGAGCAGCGCTTGTACGTCGTCGAGCAGGGTGGCCTCATGCGGCACCTGGAAGTg                          >  4:264756/1‑136 (MQ=255)
                                                                                                               ttCGATTCTCCAGAGGGGCCGCGCTGGCGGCCCCGAAAGCTCATGAAAGTTCAATGAAGCAGGCGTGCGTGAGGTTGGGTTCGAGCAGCGCTTGTACGTCGTCGAGCAGGGTGGCCTCATGCGGCACCTGGAAGTg                          >  3:107908/1‑136 (MQ=255)
                                                                                                               ttCGATTCTCCAGAGGGGCCGCGCTGGCGGCCCCGAAAGCTCATGAAAGTTCAATGAAGCAGGCGTGCGTGAGGTTGGGTTCGAGCAGCGCTTGTACGTCGTCGAGCAGGGTGGCCTCATGCGGCACCTGGAAGTg                          >  3:276235/1‑136 (MQ=255)
                                                                                                               ttCGATTCTCCAGAGGGGCCGCGCTGGCGGCCCCGAAAGCTCATGAAAGTTCAATGAAGCAGGCGTGCGTGAGGTTGGGTTCGAGCAGCGCTTGTACGTCGTCGAGCAGGGTGGCCTCATGCGGCACCTGGAAGTg                          >  4:107081/1‑136 (MQ=255)
                                                                                                               ttCGATTCTCCAGAGGGGCCGCGCTGGCGGCCCCGAAAGCTCATGAAAGTTCAATGAAGCAGGCGTGCGTGAGGTGGGGTTCGAGACGCGCTGGTCCGTCGTCGCGCAGGGTGGCCTCATGCGGCCCCTGGAAGTg                          >  1:179874/1‑136 (MQ=255)
                                                                                                               ttCGATTCTCCAGAGGCGCCGCGATGGCGGCCCCGATAGCTCATGAAAGTTCAATGAAGCAGGCGTGCCTGAGCTTGGCTTCGAGCAGCGCTTGTACGTCGTCGCGCAGGCTGGCCTCATGCGGCACCTGGaccag                          >  2:64413/1‑132 (MQ=255)
                                                                                                                 cGATCCTCCCGCGGGGCCGCGCGGGCGGCCCCGAAAGCTCATGAAAGTTCAATGAAGCAGGCGTGCGTGAGGTTGGGTTCGAGCAGCGCTTGTACGTCGTCGAGCAGGGTGGCCTCATGCGGCACCTGGAAGTGc                         <  4:150462/135‑1 (MQ=255)
                                                                                                                  gATTCTCCAGAGGGGCCGCGCTGGCGGCCCCGAAAGCTCATGAAAGTTCAATGAAGCAGGCGTGCGTGAGGTTGGGTTCGAGCAGCGCTTGTACGTCGTCGAGCAGGGTGGCCTCATGCGGCACCTGGAAGTGcc                        >  8:379364/1‑135 (MQ=255)
                                                                                                                        cccgcggggCCGCGCGGGCGGCCCCGAAAGCTCATGAAAGTTCAATGAAGCAGGCGTGCGTGAGGTTGGGTGCGAGCAGCGCTTGTACGTCGTCGAGCAGGGTGGCCTCATGCGGCACCTGGAAGTGCCCACAGGc                 <  7:87099/131‑1 (MQ=255)
                                                                                                                        cccgagGGGCCGCGCTGGCGGCCCCGAAAGCTCATGAAAGTTCAATGAAGCAGGCGTGCGTGAGGTTGGGTTCGAGCAGCGCTTGTACGTCGTCGAGCAGGGTGGCCTCATGCGGCACCTGGAAGTGCCCACAGGc                 <  3:194610/133‑1 (MQ=255)
                                                                                                                        cccgagGGGCCGCGCGGGCGGCCCCGAAAGCTCAGGAAAGTTCAATGAAGCAGGCGTGCGTGAGGTTGGGTTCGGGCAGCGCTTGTACGTCGTCGAGCAGGGTGGCCTCATGCGGCACCTGGAAGTGCCCACAGGc                 <  4:291087/133‑1 (MQ=255)
                                                                                                                        ccAGAGGGGCCGCGCTGGCGGCCCCGAAAGCTCATGAAAGTTCAATGAAGCAGGCGTGCGTGAGGTTGGGTTCGAGCAGCGCTTGTACGTCGTCGAGCAGGGTGGCCTCATGCGGCACCTGGAAGTGCCCACAGGc                 <  1:274137/136‑1 (MQ=255)
                                                                                                                         ccgagGGGCCGCGCGGGCGGCCCCGAAAGCTCATGAAAGTTCAATGAAGCAGGCGTGCGTGAGGTTGGGTTCGAGCAGCGCTTGTACGTCGTCGAGCAGGGTGGCCTCATGCGGCACCTGGAAGTGCCCACAg                   <  6:193034/131‑1 (MQ=255)
                                                                                                                         ccgagGGGCCGCGCGGGCGGCCCCGAAAGCTCATGAAAGTTCAATGAAGCAGGCGTGCGTGAGGGTGGGTTCGAGCAGCGCTTGTACGTCGTCGAGCAGGGTGGCCTCATGCGGCACCTGGAAGTGCCCACAg                   <  8:188844/131‑1 (MQ=255)
                                                                                                                          agagGGGCCGCGCTGGCGGCCCCGAAAGCTCATGAAAGTTAAATGAAGCAGGCGTGCGTGAGGTTGGGTTCGAGCAGCGCTTGTAAGTCGTCGAGCAGGGTGGCCGCATGCGGCACCTGGAAGTGCTAACAGGCca               >  1:387728/1‑136 (MQ=255)
                                                                                                                                gCCGCGCTGGCGGCCCCGAAAGCTCATGAAAGTTCAATGAAGCAGGCGTGCGTGAGGTTGGGTTCGAGCAGCGCTTGTACGTCGTCGAGCAGGGTGGCCTCATGCGGCACCTGGAAGTGCCCACAGGCCAGCAGcc         <  5:219905/136‑1 (MQ=255)
                                                                                                                                gCCGCGCGGGCGGCCCCGAAAGCTCATGAAAGTTCAATGAAGCAGGCGTGCGTGAGGTTGGGTTCGAGCAGCGCTTGTACGTCGTCGAGCAGGGTGGCCTCATGCGGCACCTGGAAGTGCCCACAGGCCAGCAGcc         <  3:245394/136‑1 (MQ=255)
                                                                                                                                gCCGCGCGGGCGGCCCCGAAAGCTCATGAAAGTTCAATGAAGCAGGCGTGCGTGAGGTTGGGGTCGAGCAGCGCTTGTACGTCGTCGAGCAGGGTGGCCTCATGCGGCACCTGGAAGTGCCCACAGGCCAGCAGcc         <  8:343467/136‑1 (MQ=255)
                                                                                                                                 ccGCGCTGGCGGCCCCGAAAGCTCATGAAAGTTCAATGAAGCAGGCGTGCGTGAGGTTGGGTTCGAGCAGCGCTTGTACGTCGTCGAGCAGGGTGGCCTCATGCGGCACCTGGAAGTGCCCACAGGCCAGccgccg        >  5:284088/1‑136 (MQ=255)
                                                                                                                                 ccGCGCTGGCGGCCCCGAAAGCTCATGAAAGTTCAATGAAGCAGGCGTGCGTGAGGTTGGGTTCGAGCAGCGCTTGTACGTCGTCGAGCAGGGTGGCCTCATGCGGCACCTGGAAGTGCCCACAGGCCAGCAGCcg        >  7:102273/1‑136 (MQ=255)
                                                                                                                                 ccGCGCTGGCGGCCCCGAAAGCTCATGAAAGTTCAAAGAAGCAGGCGTGCGTGAGGTTGGGTTCGAGCAGCGCTTGTACGTCGTCGAGCAGGGTGGCCTCATGCGGCACCTGGAAGTGCCCACCGGCCAGCAGCcg        >  4:75209/1‑136 (MQ=255)
                                                                                                                                  cgcgcgGGGGGCCCCGAAAGCTCATGAAAGTTCAATGAAGCAGGCGTGCGTGAGGTTGGGTTCGAGCAGCGCTTGTACGTCGTCGAGCAGGGTGGCCTCATGCGGCACCTGGAAGTGCCCACAGGCCAGCAGCcgc       <  2:379960/136‑1 (MQ=255)
                                                                                                                                  cgcgcgGGCGGCCCCGAAAGCTCATGAAAGTTCAATGAAGCCGGCGTGCGTGAGGTTGGGTTCGAGCAGCGCTTGTACGTCGTCGAGCAGGGTGGCCTCATGCGGCACCTGGAAGTGCCCACAGGCCAGCAGCcgc       <  8:178023/136‑1 (MQ=255)
                                                                                                                                  cgcgcgGGCGGCCCCGAAAGCTCATGAAAGTTCAATGAAGCAGGCGTGCGTGAGGTTGGGTTCGAGCAGCGCTTGTACGTCGTCGAGCAGGGTGGCCTCATGCGGCACCTGGAAGTGCCCACAGGCCAGCAGCcgc       <  3:361101/136‑1 (MQ=255)
                                                                                                                                  cgcgcgGGCGGCCCCGAAAGCTCATGAAAGTTCAATGAAGCAGGCGTGCGTGAGGTTGGGGTCGAGCAGCGCGTGTACGTCGTCGAGCAGGGTGGCCTCATGCGGCACCTGGAAGTGCCCACAGGCCAGCAGCcgc       <  7:172022/136‑1 (MQ=255)
                                                                                                                                  cgcgCTGGGGCCCCCGAAAGCTCATGAAAGTTCAATGAAGCAGGCGTGCGTGAGGTTGGGTTCGAGCAGCGCTTGTACGTCGTCGAGCAGGGTGGCCTCATGCGGCACCTGGAAGTGCCCACAGGCCAGCAGCcgc       <  3:178138/136‑1 (MQ=255)
                                                                                                                                  cgcgCTGGCTGCCCCGAAAGCTCATGAAAGTTCAATGAAGCAGGCGTGCGTGAGGTTGGGTTCGAGCAGCGCTTGTACGTCGTCGAGCAGGGTGGCCTCATGCGGCACCTGGAAGTGCCAACAGGCCAGCAGCcgc       <  8:80011/136‑1 (MQ=255)
                                                                                                                                  cgcgCTGGCGGCCCCGAAAGCTCATGAAAGTTCAATGAAGCAGGCGTGCGTGAGGTTGGGTTCGAGCAGCGCTTGTACGTCGTCGAGCAGGGTGGCCTCATGCGGCACCTGGAAGTGCCCACAGGCCAGCAGCcgc       <  5:346730/136‑1 (MQ=255)
                                                                                                                                  cgcgCTGGCGGCCCCGAAAGCTCATGAAAGTTCAATGAAGCAGGCGTGCGTGAGGTTGGGTTCGAGCAGCGCTTGTACGTCGTCGAGCAGGGTGGCCTCATGCGGCACCTGGAAGTGCCCACAGGCCAGCAGCcgc       <  2:343867/136‑1 (MQ=255)
                                                                                                                                  cgcgCTGGCGGCCCCGAAAGCTCATGAAAGTTCAATGAAGCAGGCGTGCGTGAGGTTGGGTTCGAGCAGCGCTTGTACGTCGTCGAGCAGGGTGGCCTCATGCGGCACCTGGAAGTGCCCACAGGCCAGCAGCcgc       <  2:398378/136‑1 (MQ=255)
                                                                                                                                  cgcgCTGGCGGCCCCGAAAGCTCATGAAAGTTCAATGAAGCAGGCGTGCGTGAGGTTGGGTTCGAGCAGCGCTTGTACGTCGTCGAGCAGGGTGGCCTCATGCGGCACCTGGAAGTGCCCACAGGCCAGCAGCcgc       <  3:134218/136‑1 (MQ=255)
                                                                                                                                  cgcgCTGGCGGCCCCGAAAGCTCATGAAAGTTCAATGAAGCAGGCGTGCGTGAGGTTGGGTTCGAGCAGCGCTTGTACGTCGTCGAGCAGGGTGGCCTCATGCGGCACCTGGAAGTGCCCACAGGCCAGCAGCcgc       <  4:354876/136‑1 (MQ=255)
                                                                                                                                  cgcgCTGGCGGCCCCGAAAGCTCATGAAAGTTCAATGAAGCAGGCGTGCGTGAGGTTGGGTTCGAGCAGCGCTTGTACGTCGTCGAGCAGGGTGGCCTCATGCGGCACCTGGAAGTGCCCACAGGCCAGCAGCcgc       <  4:44891/136‑1 (MQ=255)
                                                                                                                                  cccgcgGGCGGCCCCGAAAGCTCATGAAAGTTCAATGAAGCAGGCGTGCGTGAGGTTGGGTTCGAGCAGCGCTTGTACGTCGTCGAGCAGGGTGGCCTCATGCGGCACCTGGAAGTGCCCACAGGCCAGCAGCcgc       <  2:160890/134‑1 (MQ=255)
                                                                                                                                   gcgcTGGCGGCCCCGAAAGCTCATGAAAGTTCAATGAAGCAGGCGTGCGTGAGGTTGGGTTCGAGCAGCGCTTGTACGTCGTCGAGCAGGGTGGCCTCATGCGGCACCTGGAAGTGCCCACAGGCCAGCAGCcgcg      <  8:282244/136‑1 (MQ=255)
                                                                                                                                   gcgcTGGCGGCCCCGAAAGCTCATGAAAGTTCAATGAAGCAGGCGTGCGTGAGGTTGGGTTCGAGCAGCGCTTGTACGTCGTCGAGCAGGGTGGCCTCATGCGGCACCTGGAAGTGCCCACAGGCCAGCAGCcgcg      <  1:297426/136‑1 (MQ=255)
                                                                                                                                   gcgcGGGCGGCCCCGAAAGCTCATGAAAGTTCAATGAAGCAGGCGTGCGTGAGGTTGGGTTCGAGCAGCGCTTGTACGTCGTCGAGCAGGGTGGCCTCATGCGGCACCTGGAAGTGCCCACAGGCCAGCAGCcgcg      <  1:57509/136‑1 (MQ=255)
                                                                                                                                    cgcTGGCGGCCCCGAAAGCTCATGAAAGTTCAATGAAGCAGGCGTGCGTGAGGTTGGGTTCGAGCAGCGCTTGTACGTCGTCGAGCAGGGTGGCCTc                                            >  4:244709/1‑97 (MQ=255)
                                                                                                                                    cgcTGGCGGCCCCGAAAGCTCATGAAAGTTCAATGAAGCAGGCGTGCGTGAGGTTGGGTTCGAGCAGCGCTTGTACGTCGTCGAGCAGGGTGGCCTCATGCGGCACCTGGAAGTGCCCACAGGCCAGCAGcc         <  2:97650/132‑1 (MQ=255)
                                                                                                                                    cgcTGGCGGCCCCGAAAGCTCATGAAAGTTCAATGAAGCAGGCGTGCGTGAGGTTGGGTTCGAGCAGCGCTTGTACGTCGTCGAGCAGGGTGGCCTCATGCGGCACCTGGAAGTGCCCACAGGCCAGCAGCcgcg      >  8:207952/1‑135 (MQ=255)
                                                                                                                                    cgcTGGCGGCCCCGAAAGCTCATGAAAGTTCAATGAAGCAGGCGTGCGTGAGGTTGGGTTCGAGCAGCGCTTGTACGTCGTCGAGCAGGGTGGCCTCATGCGGCACCTGGAAGTGCCCACAGGCCAGCAGCcgcg      >  8:57454/1‑135 (MQ=255)
                                                                                                                                    cgcTGGCGGCCCCGAAAGCTCATGAAAGTTCAATGAAGCAGGCGTGCGTGAGGTTGGGTTCGAGCAGCGCTTGTACGTCGTCGAGCAGGGTGGCCTCATGCGGCACCTGGAAGTGCCCACAGGCCAGCAGCcgcg      >  1:20555/1‑135 (MQ=255)
                                                                                                                                    cgcTGGCGGCCCCGAAAGCTCAAGAAAGTTCAAAGAAGCAGGCGTGCGTGAGGTTGGGTTCGAGCCGCGCTTGTACGTCGTCGAGCAGGGTGGCCTCATGCGGCAGCTGGAAGTGCCCACAGGCCAGCAGCcgcg      >  1:103465/1‑135 (MQ=255)
                                                                                                                                      cTGGCGGCCCCGAAAGCTCATGAAAGTTCAATGAAGCAGGCGTGCGTGAGGTTGGGTTCGAGCAGCGCTTGTACGTCGTCGAGCAGGGTGGCCTCATGCGGCACCTGGAAGTGCCCACAGGCCAGCAGCCGCGCAg   >  5:94531/1‑136 (MQ=255)
                                                                                                                                       tGGCGGCCCCGAAAGCTCATGAAAGTTCAATGAAGCAGGCGTGCGTGAGGTTGGGTTCGAGCAGCGCTTGTACGTCGTCGAGCAGGGTGGCCTCATGCGGCACCTGGAAGTGCCCACAGGCCAGCAGCCGCGCAgt  <  8:303119/136‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                       
GGCGGCGCGCCTCGTCCGCCGCCAGGGCATGCATGGGCAGCACCTTGCCGGCGCTGCGTCCGGCCTCCACCAGTTGCAGGTAGGCCGCCAGGTCAGGGTTCAGGGACATCGTTCGATTCTCCAGAGGGGCCGCGCTGGCGGCCCCGAAAGCTCATGAAAGTTCAATGAAGCAGGCGTGCGTGAGGTTGGGTTCGAGCAGCGCTTGTACGTCGTCGAGCAGGGTGGCCTCATGCGGCACCTGGAAGTGCCCACAGGCCAGCAGCCGCGCAGT  >  ACB143/4761843‑4762113

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: