Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 4,840,424 T→G 8.6% W620G (TGG→GGG)  copA_3 → putative copper‑importing P‑type ATPase A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1434,840,4240TG 8.6% 580.5 / 3.6 298W620G (TGG→GGG) copA_3putative copper‑importing P‑type ATPase A
Reads supporting (aligned to +/- strand):  ref base T (130/122);  new base G (23/13);  total (163/135)
Fisher's exact test for biased strand distribution p-value = 2.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 307 total aligned reads displayed.

GGGGTGGTCGACGGCCAGCGCCTGCGCATTGGTCAGGCTACCTTCGTCTGCGCCTTGAGTGGTGCGGAAATCCCCGCTGTACCCGAGCCGCGCGGCCAGTGGCTGCTGCTGGGCGACCGCCAGGGGCCACTGGCCTGGTTCGGCCTGGATGACCGCCTGCGCGACGATGCCCCGGCCCTGCTCGCTGCCTGCAAGGCGCGGGGCTGGCACACCCTGCTGCTGTCGGGCGACAGCTCGCCGATGGTTGCCGAAGTGGCCGCGCAGCTGGGTA  >  ACB143/4840289‑4840559
                                                                                                                                       |                                                                                                                                       
ggggTGGTCGACGGCCAGCGCCTGCGCATTGGTCAGGCTACCTTCGTCTGCGCCTTGAGTGGTGCGGAAATCCCCGCTGTACCCGAGCCGCGCGGCCAGTGGCTGCTGCTGGGCGACCGCCAGGGGCCACTGGCCt                                                                                                                                         >  1:342036/1‑136 (MQ=255)
 gggTGGTCGACGTCCAGCGCCTGCGCATTGGTCAGGCTACCTTCGTCTGCGCCTTGAGTGGTGCGGAAATCCCCGCTGTACCCGAGCCGCGCGGCCAGTGGCTGCTGTTGGGCGACCGCCAGGGGCCACTGGCCTg                                                                                                                                        <  5:367899/136‑1 (MQ=255)
 gggTGGTCGACGGCCAGCGCCTGCGCATTGGTCAGGCTACCTTCGTCTGCGCCTTGAGTGGTGCGGAAATCCCCGCTGTACCCGAGCCGCGCGGCCAGTGGCTGCTGCTGGGCGACCGCCAGGGGCCACTGGCCTg                                                                                                                                        <  8:2749/136‑1 (MQ=255)
     ggtCGACGGCCAGCGCCTGCGCATTGGTCAGGCTACCTTCGTCTGCGCCTTGAGTGGTGCGGAAATCCCCGCTGTACCCGAGCCGCGCGGCCAGTGGCTGCTGCTGGGCGGCCGCCCGGGGCCCCTGGCCGTGTTc                                                                                                                                    >  8:359794/1‑136 (MQ=255)
     ggtCGACGGCCAGCGCCTGCGCATTGGTCAGGCTACCTTCGTCTGCGCCTTGAGTGGTGCGGAAATCCCCGCTGTACCCGAGCCGCGCGGCCAGTGGCTGCTGCTGGGCGACCGCCAGGGGCCACTGGCCTGGTTc                                                                                                                                    >  2:212796/1‑136 (MQ=255)
     ggtCGACGGCCAGCGCCTGCGCATTGGTCAGGCTACCTTCGTCTGCGCCTTGAGTGGTGCGGAAATCCCCGCTGTACCCGAGCCGCGCGGCCAGTGGCTGCTGCTGGGCGACCGCCAGGGGCCACTGGCCTGGTTc                                                                                                                                    >  8:44950/1‑136 (MQ=255)
     ggtCGACGGCCAGCGCCTGCGCATTGGTCAGGCTACCTTCGTCTGCGCCTTGAGTGGTGCGGAAATCCCCGCTGTACCCGAGCCGCGCGGCCAGTGGCGGCTGCTGGGCGCCCGCCAGGGGCCACTGGCCGGGTTc                                                                                                                                    >  8:355507/1‑136 (MQ=255)
      gtCGACGGCCAGCGCCTGCGCATTGGTCAGGCTACCTTCGTCTGCGCCTTGAGTGGTGCGGAAATCCCCGCTGTACCCGAGCCGCGCGGCCAGTGTCTGCGGCTGGGCGACCGCCAGGGGCCACTGGCCTGGTTc                                                                                                                                    <  1:105362/135‑1 (MQ=255)
      gtCGACGGCCAGCGCCTGCGCATTGGTCAGGCTACCTTCGTCTGCGCCTTGAGTGGTGCGGAAATCCCCGCTGTACCCGAGCCGCGCGGCCAGTGGCTGCTGCTGGGCGACCGCCAGGGGCCACTGGCCTGGTTc                                                                                                                                    <  5:381308/135‑1 (MQ=255)
                 gcgcctgcgcctTGGTCAGGCTACCTTCGTCTGCGCCTTGAGTGGTGCGGAAATCCCCGCTGTACCCGCGCCGCGCGGCCAGTGGCTGCTGCTGGGCGACCGCCAGTGGCCAATGGACTTGTTCGGCCTGGATGAc                                                                                                                        <  7:38231/136‑1 (MQ=255)
                    ccTGCGCATTGGTCAGGCTACCTTCGTCTGCGCCTTGAGTGGTGCGGAAATCCCCGCTGTACCCGAGCCGCGCGGCCAGTGGCTGCTGCTGGGCGACCGCCAGGGGCCACTGGCCTGGTTAGGCCTGGATGACCGc                                                                                                                     >  7:177433/1‑136 (MQ=255)
                        cgcagcggTCAGTCTAACTTCCGCTGCGCCTTGAGTGGTGCGGAAATCCCCGCTGTACCCGAGCCGCGCGGCCAGTGGCGGCGGCTGGGCGACCGCCAGGGGCCACTGGCCTGGTTCGGCCTGGATGACCGCCt                                                                                                                   <  8:183395/128‑1 (MQ=255)
                        cgcATTGGTCAGGCTACCTTCGTCTGCGCCTTGAGTGGTGCGGAAATCCCCGCTGTACCCGAGCCGCGCGGCCAGTGGCTGCTGCTGGGCGACCGCCAGGGGCCACTGGCCTGGTTCGGCCTGGATGACCGCCt                                                                                                                   <  1:257484/134‑1 (MQ=255)
                        cgcATTGGTCAGGCTACCTTCGTCTGCGCCTTGAGTGGTGCGGAAATCCCCGCTGTACCCGAGCCGCGCGGCCAGTGGCTGCTGCTGGGCGACCGCCAGGGGCCACTGGCCTGGTTCGGCCTGGATGACCGCCt                                                                                                                   <  1:24117/134‑1 (MQ=255)
                         gcATTGGTCAGGCTACCTTCGTCTGCGCCTTGAGTGGTGCGGAAATCCCCGCTGTACCCGAGCCGCGCGGCCAGGGGCTGCTGCTGGGCGACCGCCAGGGGCCACTGGCCTGGTTCGGCCTGGATGACCGCCTgcg                                                                                                                <  1:148972/136‑1 (MQ=255)
                          cATTGGTCAGGCTACCTTCGTCTGCGCCTTGAGTGGTGCGGAAATCCCCGCTGTACCCGAGCCGCGCGGCCAGTGGCTGCTGCTGGGCGACCGCCAGGGGCCACTGGCCTGGTTCGGCCTGGATGACCGCCTgcgc                                                                                                               <  3:244494/136‑1 (MQ=255)
                          cATTGGTCAGGCTACCTTCGTCTGCGCCTTGAGTGGTGCGGAAATCCCCGCTGTACCCGAGCCGCGCGGCCAGTGGCTGCTGCTGGGCGACCGCCAGGGGCCACTGGCCTGGTTCGGCCTGGATGACCGCCTgcgc                                                                                                               <  3:325495/136‑1 (MQ=255)
                           aTTGGTCAGGCTACCTTCGTCTGCGCCTTGAGTGGTGCGGAAATCCCCGCTGTACCCGAGCCGCGCGGCCCGTGGCTGCTGCTGGGGGCCCGCCAGGGGCCACTGGCCTGGTTAGGCCTGGATGACCGCCTGcgcg                                                                                                              >  6:290438/1‑136 (MQ=255)
                           aTTGGTCAGGCTACCTTCGTCTGCGCCTTGAGTGGTGCGGAAATCCCCGCTGTACCCGAGCCGCGCGGCCAGTGGCTGCGGCTGGGCGCCCGCCAGGGGCCACTGGCCGGGTTAGGGCGGGATGGCCGCCGGcgcg                                                                                                              >  3:258727/1‑136 (MQ=255)
                            ttGGTCAGGCTACCTTCGTCTGCGCCTTGAGTGGTGCGGAAATCCCCGCTGTACCCGAGCCGCGCGGCCAGTGGCTGCTGCTGGGCGACCGCCAGGGGCCACTGGCCTGGTTCGGCCTGGATGACCGCCTGCGcga                                                                                                             <  1:62804/136‑1 (MQ=255)
                             tGGTCAGGCTACCTTCGTCTGCGCCTTGAGTGGTGCGGAAATCCCCGCTGTACCCGAGCCGCGCGGCCAGTGGCTGCTGCTGGGCGACCGCCAGGGGCCACTGGCCTGGTTCGGCCTGGATGACCGCCTGCGcga                                                                                                             <  4:332584/135‑1 (MQ=255)
                                 cAGTCTACCTTCGTCTGCGCCTTGAGTGGTGCGGAAATCCCCGCTGTACCCGAGCCGCGCGGCCAGTGGCGGCTGCTGGGCGACCGCCAGGGGCCACTGGCCTGGTTCGGCCTGGATGACCGCCTGCGCGACGATg                                                                                                        <  2:260910/136‑1 (MQ=255)
                                  aGGCTACCTTCGTCTGCGCCTTGAGTGGTGCGGAAATCCCCGCTGTACCCGAGCCGCGCGGCCAGTGGCTGCTGCTGGGCGACCGCCAGGGGCCACTGGCCTGGTTCGGCCTGGATGACCGCCTGCGCGACGATg                                                                                                        <  7:330989/135‑1 (MQ=255)
                                     cTACCTTCGTCTGCGCCTTGAGTGGTGCGGAAATCCCCGCTGTACCCGAGCCGCGCGGCCAGTGGCTGCTGCTGGGCGACCGCCAGGGGCCACTGGCCTGGTTCGGCCTGGATGACCGCCTGCGCGCCGATGcccc                                                                                                    >  8:374864/1‑136 (MQ=255)
                                       aCCTTCGTCTGCGCCTTGAGTGGTGCGGAAATCCCCGCTGTACCCGAGCCGCGCGGCCAGTGGCTGCTGCTGGGCGCCCGCCAGGGGCCACTGGCCGGGTTCGGCCTGGATGCCCGCCTGCGCGACGATGCCCCgg                                                                                                  >  7:73919/1‑136 (MQ=255)
                                       aCCTTCGTCTGCGCCTTGAGTGGTGCGGAAATCCCCGCTGTACCCGAGCCGCGCGGCCAGTGGCTGCAGCTGGGCGAACCCCAGGGGCCACAGGCCTGGTTCCGCCTGGATGACAGCCGGCGCGCCGATGcccccg                                                                                                  >  4:214904/1‑134 (MQ=255)
                                            cGTCTGCGCCTTGAGTGGTGCGGAAATCCCCGCTGTACCCGAGCCGCGCGGCCCGTGGCTGCTGCTGGGGGCCCGCCCGGGGCCACAGGCCGGGGTCGGCCGGGCTGACCCCCGGCGCGCCCATGCCCCGGcccgg                                                                                             >  2:13928/1‑134 (MQ=255)
                                            cGTCTGCGCCTTGAGTGGTGCGGAAATCCCCGCTGTACCCGAGCCGCGCGGCCAGTGGGGGCGGGGGGGCGCCCCCCCCGGGGCACAGGCCGGGGTGGGGCGGGGGTACCCCCAGCGCGCCCATGCCCCCGccccg                                                                                             >  5:253437/1‑134 (MQ=255)
                                            cGTCTGCGCCTTGAGTGGTGCGGAAATCCCCGCTGTACCCGAGCCGCGCGGCCAGTGGCTGGTGCTGGGCGCCCGCCAGGGGCCACAGGCCGGGTTAGGCCTGGATGACCCCCGGCGCGCCCATTCCCCCGccccg                                                                                             >  3:20610/1‑134 (MQ=255)
                                            cGTCTGCGCCTTGAGTGGTGCGGAAATCCCCGCTGTACCCGAGCCGCGCGGCCAGTGGCTGCTGCTGGGCGACCGCCAGGGGCCACAGGCCTGGTTCGGCCGGGATGACCCCCAGCGCGACGATGCCCCCGCCCTg                                                                                             >  4:196451/1‑136 (MQ=255)
                                            cGTCTGCGCCTTGAGTGGTGCGGAAATCCCCGCTGTACCCGAGCCGCGCGGCCAGTGGCTGCGGCTGGGCGCCCGCCAGGGGCCACTGGCCTGGTTCGGCCTGGATGACCGCCTGCGCGACGATGCCCCGGCCCTg                                                                                             >  5:298674/1‑136 (MQ=255)
                                                 gcgcCTTGAGTGGTGCGGAAATCCCCGCTGTACCCGCGCCGCGCGGCCAGTGGCTGCTGCTGGGCGACCGCCAGGGGCCACTGGCCGGGGTCGGCCTGGATGACCCCCTGCGCGCCGATGCCCCGGCCCTGCTCGc                                                                                        >  2:49631/1‑136 (MQ=255)
                                                 gcgcCTTGAGTGGTGCGGAAATCCCCGCTGTACCCGAGCCGCGCGGCCAGTGGCTGCTGCTGGGCGACCGCCAGGGGCCCCAGGCCTGGTTCGGCCTGGATGACCGCCTGCGCGCCGATTCCCCGGCCCAGCTCg                                                                                         >  4:90248/1‑135 (MQ=255)
                                                   gcCTTGAGTGGTGCGGAAATCCCCGCTGTACCCGAGCCGCGCCGCCAGTGGCTGCTGCTGGGCGCCCGCCAGGGGCCCCAGGCCGGGTTAGGCCTGGATGACCCCCAGCGCGCCGATGCCCCCGCCCGGCACGctg                                                                                      >  6:84722/1‑136 (MQ=255)
                                                     cTTGAGTGGTGCGGAAATCCCCGCTGTACCCGAGCCGCGCGGCCAGTGGCTGCTGCTGGGCGCCCGCCAGGGGCCACTTGCCTGGTTCGGCCTGGATGACCGCCTGCGCGACGATGCCCCGGCCCTGCTCGctgcc                                                                                    >  6:166110/1‑136 (MQ=255)
                                                     cTTGAGTGGTGCGGAAATCCCCGCTGTACCCGAGCCGCGCGGCCAGTGGCTGCTGCTGGGCGACCGCCAGGGGCCACTGGCCTGGTTCGGCCTGGATGACCGCCTGCGCGACGATGCCCCGGCCCTGCTCGctgcc                                                                                    >  1:293583/1‑136 (MQ=255)
                                                     cTTGAGTGGTGCGGAAATCCCCGCTGTACCCGAGCCGCGCGGCCAGTGGCTGCTGCTGGGCGACCGCCAGGGGCCACTGGCCTGGTTCGGCCTGGATGACCGCCTGCGCGACGATGCCCCGGCCCTGCTCGctgcc                                                                                    >  7:195498/1‑136 (MQ=255)
                                                     cTTGAGTGGTGCGGAAATCCCCGCTGTACCCGAGCCGCGCGGCCAGTGGCTGCTGCTGGGCGACCGCCAGGGGCCACTGGCCTGGTTCGGCCTGGATGACCGCCTGCGCGACGATGCCCCGGCCCTGCTCGctgcc                                                                                    >  7:290299/1‑136 (MQ=255)
                                                     cTTGAGTGGTGCGGAAATCCCCGCTGTACCCGAGCCGCGCGGCCACTGGCTGCTGCTGGGCGACCGCCAGGGGCCACTGGCCTGGTTCCGCCAGGATGAACGCCTGCGCGACGATGCCCCGGCCCTTCTCGctgcc                                                                                    >  6:132910/1‑136 (MQ=255)
                                                          gtggtgCGGAAATCCCCGCTGTACCCGAGCCGCGCGGCCCGTGGCTGCTGCTGGGCGACCGCCAGGGGCCACTTGGCTGGGTAGGCCTGGAAGCCCGCCTGCGCGACGATGCCCCGGCCCGGCTCGctgcctgg                                                                                 >  2:362251/1‑133 (MQ=255)
                                                          gtggtgCGGAAATCCCCGCTGTACCAGAGCCGCGCGGCCAGTGGCAGCTGCTTGGCCACCCACACGGGCCAATGGCCAGGTTCGGCCAGGATGACCCCCTGCGCTACGATGCCCCGGCCCTGCTCGctgcctgc                                                                                 >  5:189854/1‑134 (MQ=255)
                                                            ggtgCGGAAATCCCCGCTGTACCCGAGCCGCGCGGCCAGTGGCTGCTGCTGGGCGACCGCCAGGGGCCACTGGCCTGGTTCGGGCGGGATGACCGCCTGCGCGACGATGCCCCGGCCCTGCTCGCTGCCTGCAAgg                                                                             >  8:16635/1‑136 (MQ=255)
                                                            ggggCGGAATTCCCCGCTGTCCCCGAGCCGCGCGGCCTGTGGCTGCTGCTGGGCGACCGCCAGGGGCCACTGGCCGGGTTGGGCCTGGATGACCGCCTGCGCGACGATGCCCCGGCCCTGCTCGCTGCCTGCAAgg                                                                             <  4:364788/133‑1 (MQ=255)
                                                             gggCGGAAATCCCCGCTGTACCCGAGCCGCGCGGCCAGTGGCTGCTGCTGGGCGACCGCCAGGGGCCACTGGCCTGGTTCGGCCTGGATGACCGCCTGCGCGACGATGCCCCGGCCCTGCTCGCTGCCTGCAAgg                                                                             <  4:273276/133‑1 (MQ=255)
                                                              tgCGGAAATCCCCGCTGTACCCGAGCCGCGCGGCCAGGGGCTGCGGCGGGGCGACCGCCAGGGGCCACTGGCCTGGTTCGGCCTGGATGACCGCCTGCGCGACGATGCCCCGGCCCTGCTCGCTGCCTGCAAGgcg                                                                           <  2:200882/136‑1 (MQ=255)
                                                                 ggAAATCCCCGCTGTACCCGAGCCGCGCGGCCCGTGGCTGCGGCTGGGCCACCCCCACGGGCCACTGGCCAGGTTCGGCCCGGCTTACCGCCAGCGCGACGAAGCCCCGGCCCTGCTCCCAGCCGGCAAGgcgccg                                                                        >  4:301441/1‑134 (MQ=255)
                                                                 ggAAATCCCCGCTGTACCCGAGCCGCGCGGCCAGTGGCTGCTGCTGGGCGACCGCCAGGGGCCACTGGCCTGGTTCGGCCTGGATGACCGCCTGCGCGACGATGCCCCGGCCCTGCTCGCTGCCTGCAAGGCGCgg                                                                        >  8:305956/1‑136 (MQ=255)
                                                                 ggAAATCCCCGCTGTACCCGAGCCGCGCGGCCAGTGGCTGCTGCTGGGCGACCGCCAGGGGCCACTGGCCTGGTTCGGCCTGGATGACCGCCTGCGCGACGATGCCCCGGCCCTGCTCGCTGCCTGCAAGGCGCgg                                                                        >  4:293034/1‑136 (MQ=255)
                                                                 ggAAATCCCCGCTGTACCCGAGCCGCGCGGCCAGTGGCTGCTGCTGGGCGACCGCCAGGGGCCACTGGCCTGGTTCGGCCTGGATGACCGCCTGCGCGACGATGCCCCGGCCCTGCTCGCTGCCTGCAAGGCGCgg                                                                        >  6:386260/1‑136 (MQ=255)
                                                                 ggAAATCCCCGCTGTACCCGAGCCGCGCGGCCAGTGGCTGCTGCTGGGCGACCGCCAGGGGCCACAGGCCTGGTTCGGCCTGGATTACCGCCTGCGCGACGATGCCCCGGCCCTGCTCGCTGCCTGCAAGGCGCgg                                                                        >  4:102218/1‑136 (MQ=255)
                                                                  gAAATCCCCGCTGTACCCGAGCCGCGCGGCCAGTGGCTGCTGCTGGGCGACCGCCAGGGGCCACTGGCCTGGTTCGGCCTGGATGACCGCCTGCGCGACGATGCCCCGGCCCTGCTCGCTGCCTGCAAGGCGCggg                                                                       >  8:171612/1‑136 (MQ=255)
                                                                      tgcccGCTGTACCCGAGCCGCGCGGCCTGGGGCTGCTGCTGGGCGACCGCCAGGGGCCACTGGCCGGGGTCGGCCTGGATGACCTCCGGCGCGACGATGCCCCGGCCCTGCTCGCTGCCTGCAAGGCGGGGGGCTg                                                                   <  7:222059/134‑1 (MQ=255)
                                                                      tCCCCGCTGTACCCGAGCCGCGCGGCCCGTGGCTGCTGCTGGGCGACCGCCCGGGGCCCCAGGCCTGGTTCGGCCTGGTTGACCCCCTGCGCGACGATGCCCCGGCCCTGCGCGCTGCCTGCAAGGCGCGGGGCTg                                                                   >  6:327727/1‑136 (MQ=255)
                                                                      tCCCCGCTGTACCCGAGCCGCGCGGCCAGTGGCTGCTGCTGGGCGACCGCCAGGGGCCACTGGCCTGGTTc                                                                                                                                    <  2:93819/71‑1 (MQ=255)
                                                                      tCCCCGCTGTACCCGAGCCGCGCGGCCAGTGGCTGCTGCTGGGCGACCGCCAGGGGCCACTGGCCTGGTTc                                                                                                                                    >  1:93819/1‑71 (MQ=255)
                                                                      tCCCCGCTGTACCCGAGCCGCGCGGCCAGTGGCTGCTGCTGGGCGACCGCCAGGGGCCACTGGCCTGGTTCGGCCTGGATGACCGCCTGCGCGACGATGCCCCGGCCCTGCTCGCTGCCTGCAAGGCGCGGGGCTg                                                                   <  1:364480/136‑1 (MQ=255)
                                                                      tCCCCGCTGTACCCGAGCCGCGCGGCCAGTGGCTGCTGCTGGGCGACCGCCAGGGGCCACTGGCCTGGTTCGGCCTGGATGACCGCCTGCGCGACGATGCCCCGGCCCTGCTCGCTGCCTGCAAGGCGCGGGGCTg                                                                   <  3:113644/136‑1 (MQ=255)
                                                                        cccGCTGTCCCCGAGCCGCGCGGCCAGTGGCTGCTGCTGGGCGACCGCCAGGGGCCACTGGCCTGGTTCGGCCTGGATGACCGCCTGCGCGACGATGCCCCGGCCCTGCTCGCTGCCTGCAAGGCGCGGGGCTGGc                                                                 <  8:12718/136‑1 (MQ=255)
                                                                           gCTGTACCCGAGCCGCGCGGCCAGTGGCTGCTGCTGGGCGCCCGCCAGGGGCCACTGGCCTGGTTCGGCCTGGATGACCGCCTGCGCGACGATGCCCCCGCCCAGCTCCCTGCCTGCAAGGCGCGGGGCTGGcacc                                                              >  2:286915/1‑135 (MQ=255)
                                                                           gCTGTACCCGAGCCGCGCGGCCAGTGGCTGCTGCTGGGCGACCGCCAGGGGCCACTGGCCTGGTTCGGCCTGGATGACCGCCTGCCCGCCGCTGCCCCGGCCCTGCACGCTGCCTGCAAGGCGCGGGGCTGGcaca                                                              >  2:114158/1‑136 (MQ=255)
                                                                                  ccGAGCCGCGCGGCCAGTGGCTGCTGCTGGGCGACCGCCAGGGGCTACTGGCCTg                                                                                                                                        >  7:114419/1‑55 (MQ=255)
                                                                                  ccGAGCCGCGCGGCCAGTGGCTGCTGCTGGGCGACCGCCAGGGGCTACTGGCCTg                                                                                                                                        <  8:114419/55‑1 (MQ=255)
                                                                                    gAGCCGCGCGGCCAGTGGCTGCTGCTGGGCGACCGCCAGGGGCCACTGGCCTGGTTCGGCCTGGATGACCGCCTGCGCGACGATGCCCCGGCCCTGCTCGCTGCCTGCAAGGCGCGGGGCTGGCACCCCctgctgc                                                     >  5:93577/1‑136 (MQ=255)
                                                                                    gAGCCGCGCGGCCAGTGGCTGCTGCTGGGCGACCGCCAGGGGCCACTGGCCTGGTTCGGCCTGGATGACCGCCTGCGCGACGATGCCCCGGCCCTGCTCGCTGCCTGCAAGGCGCGGGGCTGGCACACCctgctgc                                                     >  5:164873/1‑136 (MQ=255)
                                                                                       ccGCGCGGCCCGTGGCTGCTGCTGGGCGACCGCCAGGGGCCACTGGGCTGGTTAGGCCGGGATGACCGCCTGGGCGACGATGCCCCGGCCCTGCTCGCTGCCTGCAAGGCGCGGGGCGGGGACCCCCGGCTGCTGt                                                  >  8:65472/1‑136 (MQ=255)
                                                                                          cgcgGCCAGTGGCTGCTGCTGGGCGACCGCCAGGGGCCACTGGCCTGGTTCGGCCTGGATGACCGCCTGCGCGCCGCTGCCCCGGCCCTGCTCGCTGCCTGCAAGGCGCGGGGCTGGCACACCCTGCTGCTGGCgg                                               >  7:342967/1‑136 (MQ=255)
                                                                                           gcgGCCAGTGGCTGCTGCTGGGCGACCGCCAGGGGCCACTGGCCTGGTTCGGCCTGGATGACCGCCTGCGCGACGATGCCCCGGCCCTGCTCGCTGCCTGCAAGGCGCGGGGCTGGCACACCCTGCTGCTGTCggg                                              <  5:356566/136‑1 (MQ=255)
                                                                                            cgGCCCGTGGCTGCGGCTGGGCGCCCCCCAGGGGCCACTGGCCTGGTTCGGCCGGGATGACCGCCTGCGCGCCGATTCCCCGGCCCAGCTCGCTGCCTGCAAGGCGCGGGGCTGGCCCACCCGGCTGCTGTCGGGc                                             >  4:154625/1‑136 (MQ=255)
                                                                                            cgGCCAGTGGCTGCTGCTGGGCGCCCGCCAGGGGCCACTGGCCTGGTTCGGCCTGGATGACCGCCTGCGCGACGATGCCCCGGCCCTGCTCGCTGCCTGCAAGGCGCGGGGCTGGCACACCCTGCTGCTGTCGGGc                                             >  6:68585/1‑136 (MQ=255)
                                                                                            cgGCCAGTGGCTGCGGCTGGGCGACCGCCAGGGGCCACTGGCCTGGTTCGGCCTGGATGACCGCCTGCGCGACGATGCCCCGGCCCTGCTCGCTGCCTGCAAGGCGCGGGGCTGGCACACCCTGCTGCTGTCGGGc                                             <  3:136425/136‑1 (MQ=255)
                                                                                              gCCCGTGGCTGCTGCTGGGCGACCGCCAGGGGCCACTGGCCTGGTTCGGGCTGGATGACCGCCTGCGCGACGCTGCCCCGGCCCTGCTCGCGGCCTGCAAGGCGCGGGGCTGGCCCCCCCTGCTGCTGTCGGGCgc                                           >  5:116771/1‑135 (MQ=255)
                                                                                              gCCAGTGGCTGCTGCTGGGCGACCGCCAGGGGCCACTGGCCTGGTTCGGCCTGGATGACCGCCTGCGCGACGATGCCCCGGCCCTGCTCGCTGCCTGCAAGGCGCGGGGCTGGCACACCCTGCTGCTGTCGGGCGa                                           <  5:310972/136‑1 (MQ=255)
                                                                                                     gctgctgcgGGGCGACCGCCAGGGGCCACTGGCCGGGTTCGGCCTGGATGACCGCCTGCGCGACGATGCCCCGGCCCTGCTCGCGGCCTGCAAGGCGCGGGGCTGGCACACCCTGCTGCTGTCGGGCGACAGCTCg                                    <  5:40704/136‑1 (MQ=255)
                                                                                                     gctgctgcaGGGCGACCGCCAGGGGCCACTGGCCAGGTTCGGCCTGGATGACCCCCAGCGCGACGATGCCCCGGCCCTGCTCGCATCCTGCAAGGCGCGGGGCTGGCACACCCTGCTGCTGTCGGGCGACAGCACg                                    >  2:328247/1‑136 (MQ=255)
                                                                                                       tgctgctgGGCGACCGCCAGGGGCCACTGGCCTGGTTCGGCCTGGATGACCGCCTGCGCGACGATGCCCCGGCCCTGCTCGCTGCCTGCAAGGCGCGGGGCTGGCACACCCTGCTGCTGTCGGGCGACAGCTCGcc                                  <  2:66263/136‑1 (MQ=255)
                                                                                                            ctgGGCGACCGCCAGGGGCCACTGGCCTGGTTCGGCCTGGATGACCGCCTGCGCGACGATGCCCCGGCCCTGCTCGCTGCCTGCAAGGCGCGGGGCTGGCACCCCCTGCTGCTGTCGGGCGACAGCTCGCCGATgg                             >  2:82724/1‑136 (MQ=255)
                                                                                                            ctgGGCGACCGCCAGGGGCCACTGGCCTGGTTCGGCCTGGATGACCGCCTGCGCGACGATGCCCCGGCCCTGCTCGCTGCCTGCAAGGCGCGGGGCTGGCACACCCTGCTGCTGTCGGGCGACAGCTCGCCGATgg                             >  6:277962/1‑136 (MQ=255)
                                                                                                            ctgGGCGACCGCCAGGGGCCACTGGCCTGGTTCGGCCTGGATGACCGCCTGCGCGACGATGCCCCGGCCCTGCTCGCTGCCTGCAAGGCGCGGGGCTGGCACACCCTGCTGCTGTCGGGCGACAGCTCGCCGATgg                             >  1:17154/1‑136 (MQ=255)
                                                                                                                gCGACCGCCAGGGGCCACTGGCCTGGTTCGGCCTGGATGACCGCCTGCGCGACGATGCCCCGGCCCTGCTCGCTGCCTGCAAGGCGCGGGGCTGGCACACCCTGCTGCTGTCGGGCGACAGCTCGCCGATGGTTGc                         >  8:189017/1‑136 (MQ=255)
                                                                                                                  gACCGCCAGGGGCCACTGGCCTGGTTCGGCCTGGATGACCGCCTGCGCGACGATGCCCCGGCCCTGCTCGCTGCCTGCAAGGCGCGGGGCTGGCACACCCTGCTGCTGTCGGGCGACAGCTCGCCGATGGTTGcc                        >  2:232520/1‑135 (MQ=255)
                                                                                                                  gACCGCCAGGGGCCACTGGCCTGGTTCGGCCTGGATGACCGCCTGCGCGACGATGCCCCGGCCCTGCTCGCTGCCTGCAAGGCGCGGGGCTGGCACACCCTGCTGCTGTCGGGCGACAGCTCGCCGATGGTTGcc                        >  8:247251/1‑135 (MQ=255)
                                                                                                                        cAGGGGCCACTGGCCTGGTTCGGCCTGGATGACCGCCTGCGCGACGATGCCCCGGCCCTGCTCGCTGCCTGCAAGGCGCGGGGCTGGCACACCCTGCTGCTGTCGGGCGACAGCTCGCCGATGGTTGCCGAAGTgg                 <  4:356280/136‑1 (MQ=255)
                                                                                                                         aGGGGCCACTGGCCTGGTTCGGCCTGGATGACCGCCTGCGCGACGATGCCCCGGCCCTGCTCGCTGCCTGCAAGGCGCGGGGCTGGCACACCCTGCTGCTGTCGGGCGACAGCTCGCCGATGGTTGCCGAAGTGGc                >  8:344369/1‑136 (MQ=255)
                                                                                                                          ggggCGACTGGCCTGGTTCGGCGTGGATGACCGCCTGCGCGACGATACCCCGGCCCTGCTCGCTGCCTGCTAGGCGCGGGGCTGGCACACCCTGCTGCTGTCGGGCGACAGCTCGCCGATGGTTGCCGAAGTGGcc               <  1:190210/136‑1 (MQ=255)
                                                                                                                          ggggCCACTGGCCTGGTTCGGCCTGGATGACCGCCTGCGCGACGATGCCCCGGCCCTGCTCGCTGCCTGCAAGGCGCGGGGCTGGCACACCCTGCTGCTGTCGGGCGACAGCTCGCCGATGGTTGCCGAAGTGGcc               <  7:25974/136‑1 (MQ=255)
                                                                                                                          ggggCCACTGGCCTGGTTCGGCCTGGATGACCGCCTGCGCGACGATGCCCCGGCCCTGCTCGCTGCCTGCAAGGCGCGGGGCTGGCACACCCTGCTGCTGTCGGGCGACAGCTCGCCGATGGTTGCCGAAGTGGcc               <  6:367610/136‑1 (MQ=255)
                                                                                                                          ggggCCACTGGCCTGGTGCGGCCTGGATGACCGCCTGCGCGACGATGCCCCGGCCCTGCGCGCGGCCTGCAAGGCGCGGGGCTGGCACACCCTGCTGCTGTCGGGCGACAGCTCGCCGATGGTTGCCGAAGTGGcc               <  6:245244/136‑1 (MQ=255)
                                                                                                                          ggggCCACTGGCCGGGTTCGGCCTGGATGACCGCCTGCGCGACGATGCCCCGGCCCTGCTCGCTGCCTGCAAGGCGCGGGGCTGGCACACCCTGCTGCTGTCGGGCGACAGCTCGCCGATGGTTGCCGAAGTGGcc               <  3:293034/136‑1 (MQ=255)
                                                                                                                          ggggCCACTGGCCGGGTGCGGCCTGGATGACCGCCTGCGCGACGATGCCCCGGCCCTGCTCGCTGCCTGCAAGGCGCGGGGCTGGCACACCCTGCTGCTGTCGGGCGACAGCTCGCCGATGGTTGCCGAAGTGGcc               <  7:233788/136‑1 (MQ=255)
                                                                                                                           gggCCACTGGCCTGGTGCGGCCTGGATGACCGCCTGCGCGACGATGCCCCGGCCCTGCTCGCTGCCTGCAAGGCGCGGGGCTGGCACACCCTGCTGCTGTCGGGCGACAGCTCGCCGATGGTTGCCGAAGTGGcc               <  4:34473/135‑1 (MQ=255)
                                                                                                                            ggCCACTGGCCTGGTTCGGCCTGGATGACCGCCTGCGCGACGATGCCCCGGCCCTGCTCGCTGCCTGCAAGGCGCGGGGCTGGCACCCCCTGCTGCTGTCGGGCGACAGCTCGCCGATGGTTGCCGAAGTGGCcgc             >  4:52036/1‑136 (MQ=255)
                                                                                                                              ccACTGGCCTGGTGCGGCCGGGATGCCCCCCTGCGCGACGATGCCCCGGCCCTGCCCGCTCCCGGCAAGGCGCGGGGCTGGCACACCCTGCTGCTGTCGGGCGACAGCTCGCCGATGGTTGCCGAAGTGGCCgcgc           <  6:45125/136‑1 (MQ=255)
                                                                                                                               cACTGGCCTGGTTCGGCCTGGATGACCGCCTGCGCGACGATGCCCCGGCCCTGCTCGCTGCCTGCAAGGCGCGGGGCTGGCACACCCTGCTGCTGTCGGGCGACAGCTCGCCGATGGTTGCCGAAGTGGCCGCGCa          <  6:309658/136‑1 (MQ=255)
                                                                                                                               cACTGGCCTGGTTCGGCCTGGATGACCGCCTGCGCGACGATGCCCCGGCCCTGCTCGCTGCCTGCAAGGCGCGGGGCTGGCACACCCTGCTGCTGTCGGGCGACAGCTCGCCGATGGTTGCCGAAGTGGCCGCGCa          <  6:262982/136‑1 (MQ=255)
                                                                                                                                 cTGGCCTGGTTCGGCCTGGATGACCGCCTGCGCGACGATGCCCCGGCCCTGCTCGCTGCCTGCAAGGCGCGGGGCTGGCACACCCTGCTGCTGTCGGGCGACAGCTCGCCGATGGTTGCCGAAGTGGCCGCGCAGc        >  1:287227/1‑136 (MQ=255)
                                                                                                                                  tGGCCTGGTTCGGCCTGGATGACCGCCTGCGCGACGATGCCCCGGCCCTGCTCGCTGCCTGCAAGGCGCGGGGCTGGCACACCCTGCTGCTGTCGGGCGACAGCTCGCCGATGGTTGACGAAGTGGCCGCGCAGCt       >  4:77775/1‑136 (MQ=255)
                                                                                                                                    gcatGGTTAGGCCTGGATGACCGCCTTCGCGACGATGCACCTTCCCTGCTCGCTGCCTGCAAGTCGCCGGGCTGGCACACACTGCTGCTGTCTGGCTACAGCTCGCCTCAGGTTGCAGAAGTGGCCGCGCATCTgc     >  8:236029/4‑135 (MQ=255)
                                                                                                                                    gCCTGGTTCGGCCTGGATGACCGCCTGCGCGACGATGCCCCGGCCCTGCTCGCTGCCTGCAAGGCGCGGGGCTGGCACACCCTGCTGCTGTCGGGCGACAGCTCGCCGATGGTTGCCGAAGTGGCCGCGCAGCTgg     >  1:322650/1‑136 (MQ=255)
                                                                                                                                    gCCGGGTTCGGCCTGGATGACCGCCTGCGCGACGATGCCCCGGCCCTGCTCGCTGCCTGCAAGGCGCGGGGCTGGCACACCCTGCTGCTGTCGGGCGACAGCTCGCCGATGGTTGCCGAAGTGGCCGCGCAGCTgg     <  5:221976/136‑1 (MQ=255)
                                                                                                                                       tGGTTCGGCCTGGATGACCGCCTGCGCGACGATGCCCCGGCCCTGCTCGCTGCCTGCAAGGCGCGGGGCTGGCACACCCTGCTGCTGTCGGGCGACAGCTCGCCGATGGTTGCCGAAGTGGCCGCGCAGCTGGGTa  >  8:105385/1‑136 (MQ=255)
                                                                                                                                       |                                                                                                                                       
GGGGTGGTCGACGGCCAGCGCCTGCGCATTGGTCAGGCTACCTTCGTCTGCGCCTTGAGTGGTGCGGAAATCCCCGCTGTACCCGAGCCGCGCGGCCAGTGGCTGCTGCTGGGCGACCGCCAGGGGCCACTGGCCTGGTTCGGCCTGGATGACCGCCTGCGCGACGATGCCCCGGCCCTGCTCGCTGCCTGCAAGGCGCGGGGCTGGCACACCCTGCTGCTGTCGGGCGACAGCTCGCCGATGGTTGCCGAAGTGGCCGCGCAGCTGGGTA  >  ACB143/4840289‑4840559

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: