| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB143 | 5,096,415 | C→G | 25.2% | intergenic (+28/‑162) | CJ019_04792 → / → CJ019_04793 | Putative pterin‑4‑alpha‑carbinolaminedehydratase/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB143 | 5,096,415 | 0 | C | G | 25.2% | 81.6 / 7.9 | 61 | intergenic (+28/‑162) | CJ019_04792/CJ019_04793 | Putative pterin‑4‑alpha‑carbinolaminedehydratase/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (22/22); new base G (10/4); total (32/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.21e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CACCGTGACCTGGTGGAGCCACTCGATCAAAGGCCTGCACCGCAACGACTTCATCATGTGTGCACGCACTGACAAGGTGGCGGAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCCTTTTTTCATGGGGCATATGTGCGCTGTTCGCCCGCAAAACCGACAAACGACCGGGAAAAAAACCAAACTGTCATCCAGACTTGTGTATCCTGCCCGAGCTTTTTGAAGCTACAAACGCGCCTGGCGCAGAC > ACB143/5096282‑5096546 | cACCGTGACCTGGTGGAGCCACTCGATCAAAGGCCTGCACCGCAACGACTTCATCATGTGTGCACGCACTGACAAGGTGGCGGAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGccccgt > 2:383215/1‑134 (MQ=255) gttGACCTGGTGGAGGCACTCTATCAAAGGCATGAACCGCATCTACTTCTTCATGTGTGCACGCAATTACTTGGTGGCGGAAACGGCTGAAGTCCGGAAGTAAACACCTGAAGGATGGGGCCAGCGGCCCCttttt < 6:140997/134‑1 (MQ=255) cGTGACCTGGTGGAGCCACTCGATCAAAGGCCTGCACCGCAACGACTTCATCATGTGTGCACGCACTGACAAGGTGGCGGAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCCttttt < 2:145011/136‑1 (MQ=255) cGTGACCTGGTGGAGCCACTCGATCAAAGGCCTGCACCGCAACGACTTCATCATGTGTGCACGCACTGACAAGGTGGCGGAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCCttttt < 2:222485/136‑1 (MQ=255) cGTGACCTGGTGGAGCCACTCGATCAAAGGCCTGCACCGCAACGACTTCATCATGTGTGCACGCACTGACAAGGTGGCGGAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCCttttt < 4:383989/136‑1 (MQ=255) cGTGACCTGGGGGAGCCACTCGATCAAAGGCCTGCACCGCAACGACTTCATCATGTGTGAACGCACTGACAAGGTGGCGGAAACGGCTGAAGGCCGGAAGTAAAAACCAGAAGGAAGGGGCCAGAGGCCCCttttt < 4:233616/136‑1 (MQ=255) tGACCTGGTGGAGCCACTCGATCAAAGGCCTGCACCGCAACGACTTCATCATGTGTGCACGCACTGACAAGGTGGCGGAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCCTTTTTTc < 2:9105/136‑1 (MQ=255) tGACCTGGTGGAGCCACTCGATCAAAGGCCTGCACCGCAACGACTTCATCATGTGTGCACGCACTGACAAGGTGGCAGAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCCtttttt > 3:387933/1‑135 (MQ=255) ccTGGGGGAGCCACTCTATCAACGGCCTGCACCGCAACGACTTCATCATGTGTGCACGCACTGACACGGGGGCGGAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCCTTTTTTCATg < 4:10475/136‑1 (MQ=255) aGCCACTCGATCAAAGGCCTGCACCGCAACGACTTCATCATGTGTGCACGCACTGACAAGGTGGCGGAAACGGCTGAATGCCGGAAGTAAACACCAGAAGGAAGGGGTCAGAGGCCCCTTTTTTAATGGGGCatat > 6:182005/1‑136 (MQ=255) aGCCACTCGATCAAAGGCCTGCACCGCAACGACTTCATCATGTGTGCACGCACTGACAAGGTGGCGGAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCCTTTTTTAATGGGGCatat > 2:224992/1‑136 (MQ=255) aGCCACTCGATCAAAGGCCTGCACCGCAACGACTTCATCATGTGTGCACGCACTGACAAGGTGGCGGAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCCATTTTTCATGGGGCatat > 7:241003/1‑136 (MQ=255) aCTCGATCAAAGGCCTGCACCGCAACGACTTCATCATGTGTGCACGCACTGACAAGGTGGCGGAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCCTTTTTTAATGGGGCatattttc > 7:245306/1‑132 (MQ=255) cGATCAAAGGCCTGCACCGCAACGACTTCATCATGTGTGCACGCACTGACAAGGTGGCGGAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCCTTTTTTCATGGGGCATATGTGCGCt > 6:383413/1‑136 (MQ=255) aTCAAAGGCCTGCACCGCAACGACTTCATCATGTGTGCACGCACTGACAAGGTGGCGGAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCCTTTTTTCATTGGGCATATGTGCGCTGt > 2:210175/1‑136 (MQ=255) aTCAAAGGCCTGCACCGCAACGACTTCATCATGTGTGCACGCACTGACAAGGTGGCGGAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCCTTTTTTCATGGGGCATATGTGAGCTGt > 2:362163/1‑136 (MQ=255) cACCGCAACGACTTCATCATGTGTGCACGCACTGACAAGGTGGCGGAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGTAAGGGGCCAGAGGCCCCTTTTTTCATGGGGCATATGTGCGCTGTTCGCCCGCaa < 6:213057/134‑1 (MQ=255) cGCAACTAATTAATCATGTGTGCACGCACTTACATGGTGGCGGAAAGGGCTTAAGGCCGTAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCGTTTTTTCATGGGGCATATGTGCGCTGTTCGCCCGAAAAACCg < 4:193595/136‑1 (MQ=255) cGACTTCATCATGTGTGCACGCACTGACAAGGTGGCGGAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCGGTTTTTGATGGGGCATATGTGCGCTGTTCGCCCGCAAAACCGACaaa > 3:32993/1‑136 (MQ=255) cGACTTCATCATGTGTGCACGCACTGACAAGGTGGCGGAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCCGTTTTTGATGGGGCATATGTGCGCTGTTCGCCCGCAAAACCGCCaa > 8:89454/1‑135 (MQ=255) cGACTTCATCATGTGTGCACGCACTGACAAGGTGGAGGAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGACAGAGGCCCGTTTTTTGATGGGGCATATGTGCGGTGTTGGACCGCTAAACCGTCaat > 6:62585/1‑135 (MQ=255) tgCACGCACTGAAAAGGTGGCGTAAACGGCTTAAGGCCGTAAGTAAACACCATATGGAAGGGGCATGAGGCCCGTTTTTTCATGGGGATTATGTGCGCTGTTCGCCCGAAAAACCGACAAACGACCGGGaaaaaaa < 5:314643/136‑1 (MQ=255) cacgcacTTACATGGTGGGTTAAACGGCTTATGGCGGTAAGTAAACACCACACGGACGGGGCCAGAGGCCCGTTTTTTCATGGGGCATATGTGGGCTGTTCGCCCGCAAAACCGACAAACGACCGGGAAAAAAAcc < 5:288219/136‑1 (MQ=255) cacgcacTGACAAGGTGGCGGAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCGTTTTTTCATGGGGCATATGTTCGCTGTTCCCCCGCAACACCGACAAACGACCGGGAAAAAAAc > 2:384415/1‑135 (MQ=255) cacgcacTGACAAGGTGGCGGAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCGTTTTTTAATGGGGCATATGTGCGCTGTTCGCCCGCAAAACCGACAAACGACCGGGAAAAAAAc > 3:340437/1‑135 (MQ=255) acgcacTGACAAGGTGGCGGAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCCTTTTTTAATTGGGCATATGTGCGCTGTTCGCCCGAAAAACCGAAAAACGACCGGGaaaaaaaac > 1:114553/1‑133 (MQ=255) gcacTGACAAGGTGGCGGAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCGGTTTTTCATGGGGCATATGTGCGCTGTTCGCCCGCAAACCCGACAAACGACCGGGAAAAAAACCaa > 7:270811/1‑135 (MQ=255) gACAAGGTGGCGGAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCCTTTTTTCATGGGGCATATGTGCGCTGTTCGCCCGCAAAACCGACAAACGACCGGGAAAAAAACCAAACTGt > 8:12506/1‑135 (MQ=255) gACAAGGTGGCGGAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCCTTTTTTCATGGGGCATATGTGCGCTGTTCGCCCGCAAAACAGACAAACGACCGGGAAAAAAACAAAACTGt > 2:79586/1‑135 (MQ=255) gACAAGGTGGCGGAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCCTTTTTTAATGGGGCAAATTTGCGCTGTTCGCCCGCAAAACCGACAAACGACCGGGAAAAAAAACAAACTGt > 6:69399/1‑135 (MQ=255) aCAAGGTGGCGTAAAGGGCTGAAGGCCGGAAGTAAACACCAGAAGTAAGGGGCCAGAGGCCCCTTTTTTCATGGGGCATATGTGCGCTGTTCGCCCGCAAAACCGACAAACGACCGGGAAAAAAACCAAACTGTc < 7:121491/135‑1 (MQ=255) cAAGGTGGCGTAACCGGCTGAAGGCCGGAAGTAAACACCAGAAGTAAGGGGCCAGAGGCCCCTTTTTTCATGGGGCATATGTGCGCTGTTCGCCCGCAAAACCGACAAACGACCGGGAAAAAAACCAAACTGTCAt < 3:390847/136‑1 (MQ=255) cAAGGTGCCTGAAACGGTTTAAGGCCGTAATTAAACACCAGAAGGAAGGGGCCAGAGGCCCGTTTTTTCATGGGGCATATGTGCTCTGTTGGCCCGCAAAACCGACAAACGACCGGGAAAAAAACCAAACTGTCAt < 3:59446/136‑1 (MQ=255) cAAGGGGTCGTAAACGGCTGAAGGCGGTATTTAAACACCAGAAGGAAGGGGCCAGAGGCCCCTTTTTTCATGGGGCATTTGTGCGCTGTTCGCCCGCAAAACCGACAAACGACCGGGAAAAAAACCAAACTGTCAt < 7:218275/136‑1 (MQ=255) aaaGGTGGCGTAACCGGCTCAAGCCCGTAAGAAAACACCAGACGGACGGGGCCAGCGGCCCCTTTTTTCATGGGGCATATGTGCGCTGTTCGCCCGAAAAACCGACAAACGACCGGGAAAAAAACCAAACTGTCAt < 7:338725/135‑1 (MQ=255) aaGGTGGCGGAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCTAGAGGCCCGTTTTTTAATGGGGCATATGTGCGCTGTTCGCCCGCCAAACCGCCAAACGACCCGGAAAAAAACCAAACTGTCAta > 7:338045/1‑135 (MQ=255) cgttGGCGTAAACGGATGAAGGCCGTATGAAAACACATCAAGGACGGGCCCAGATCCCCTTTTTTTAAGGGGTCATATGTGCGCTGTTCGCCCGAAAAACCCACAAACGACCGGGAAAAAAACCAAACTGTCATcc < 4:81288/133‑1 (MQ=255) cggTGGCGTAAACGGCTGATGGCCGGAAGTAAACACCAGACGGCCGGGGCCAGAGGCCCCTTTTTTCATGGGGCATATGTGCGCTGTGCGCCCGAAAACCCGACAAACGACCGGGAAAAAAACCAAACTGTCATcc < 3:215793/135‑1 (MQ=255) aGGTGGCGGAACCGGGTGATGGCGGTAAGTAAACACCACACGCCGGGGGCCAGAGGCCCTTTTTTTCATGGGGCATATGTGCGCTGTGCGCCCGCAAACCCCACAAACGACCGGGAAAAAAACCAAACTGTCATcc < 1:132681/136‑1 (MQ=255) aGGTGGCGGAAACGGCTGAAGGCCGGAATTAAACACCAGAAGGAAGGGGCCAGAGGCCCCTTTTTTCATGGGGCATATGTGCGCTGTTCGCCCGCAAAACCGACAAACGACCGGGAAAAAAACCAAACTGTCATcc < 1:49399/136‑1 (MQ=255) aGGGGGCGGAAACGGCGGAAGGCCGGAAGTAACCACCAGAAGTAAGGGGCCAGAGGCGCCTTTTTTCATGTGGCATATGTGCGCTGGTCGCCGGCAAAACCGACAAACGACCGGGAAAAAAACCAAACTGTCATcc < 6:267621/136‑1 (MQ=255) gggggCGTAAACGGCTGACGGCGGTAAGAAAACACCACACGCATGGGGCCCGCGGCCCCTTTTTTCATGGGGCATATGTGCGCTGTTCGCCCCAAAAACCGACAAACGACCGGGAAAAAAACCAAACTGTCATCCa < 8:164239/133‑1 (MQ=255) ggCGGAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCCTTTTTTCATGGGGCATATGAGCGCTGTTCGCCCGCAAAACCGACAAACGCCAGGGAAAAAAACCAAACTGTCAACCacac > 8:160353/1‑136 (MQ=255) gAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCGGTTTTTCATGGGGCATATGTGCGCTGTTCGCCCGCAAAACCGACAAACGACCGGGAAAAAAACCAAACTGTCATCCAGACTtgt > 8:139473/1‑136 (MQ=255) gAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCCttttt > 5:372592/1‑57 (MQ=255) gAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCCttttt < 6:372592/57‑1 (MQ=255) aaaCGGCTGACGGCGGGATGTAAACACCATATGGAAGGGGCCAGCGGCCCCTTTTTTCATGGGGAATATGTGCGCGTTTCGCCCGAAAACCCGACAAACGACCGGGAAAAAAACCAAACTGTCATCCAGACTtgtg < 1:267283/136‑1 (MQ=255) aaCGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCCTTTTTTCATGGGGCATATGTGCGCTGTTCGCCCGCAAAACACACAAACGACCGGGAAAAAAACCAAACTGTCATCCAGACTTgtgt > 6:171199/1‑136 (MQ=255) gCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCGGTTTTTGATGGGGCATATGTGCGCTGTTCGCCCGCAAAACCGACAAACGACCGGGAAAACAACCAAACTGTCATCCAGACTTGTGTATCCTGccc > 6:319157/1‑134 (MQ=255) aGTAACCCCCAGAAGGAAGGGGCCAGAGGCCCCTTTTTTCATGGGGCATATGTGCGCGGTGCGCCCGCAAAACCGACAAACGACCGGGAAAAAAACCAAACTGTCATCCAGACTTGTGTATCCTGCCCGAGCtttt < 7:79527/136‑1 (MQ=255) acCAGAAGGAAGGGGCCAGAGGCCCGTTTTTTAAAGGGGCATATTTGCGCTGTTGGCCCCCCAAACCGACAAACGACCGGGAAAAAAACAAAACAGTCAAACAGACTTGTTTATACTGCCCGCGCGTTTTTaacg > 6:20392/1‑133 (MQ=255) aaggaaggGGCCAGAGGCCCCTTTTTTCATGGGGCATATGTGCGCTGTTCGCCCGCAAAACCGACAAACGACCGGGAAAAAAACCCAACTGTCATCCAGACTTGTGTATCCGGCCCGCGCTTTTTGAAGCTACaa > 7:179319/1‑135 (MQ=255) ggacggGGCCCGAGGCCCCTTTTTTCATGGGGCATATGTGCGCTGTTCGCCCGCAAAACCGACAAACGACCGGGAAAAAAACCAAACTGTCATCCAGACTTGTGTATCCTGCCCGAGCTTTTTGAAGCTACAAAcg < 3:304047/136‑1 (MQ=255) ggacggGGCCCGAGCCCCCTTTTTTCTTGGGGCATATGTGCGCGGTGCCCCCGCAAACCCGACAAACGACCGGGAAAAAAACCAAACTGTCATCCAGACTTGTGTATCCTGCCCGAGCTTTTTGAAGCTACAAAcg < 4:318610/136‑1 (MQ=255) cacgggcCCACAGCCCCATTTTTTATTGGGGCATATGGGCGCTGTCCGCCCGCAAACCCCAAAAACGACCGGGAAAAAAACCAAACTGTCATCCAGACTTGTGTATCCTGCCCGAGCTTTTTGAAGCTACAAAcgc < 5:111594/133‑1 (MQ=255) ggCCAGAGGCCCCTTTTTTCATGGGGCATATGTGCGCTGTTCGCCCGCAAAACCGACAAACGCCCGGGAAAAAAACCAAACTGTCATCCAGACTTGTGTATCCTGCCCGAGCTTTTTGAAGCTACAAACGCGccgg > 2:39088/1‑134 (MQ=255) ggCCAGAGGCCCCTTTTTTCATGGGGCATATGTGCGCTGTTCGCCCGCAAAACCGACAAACGACCGGGAAAAAAACCAAACTGTCATcc < 7:243698/89‑1 (MQ=255) ggCCAGAGGCCCCTTTTTTCATGGGGCATATGTGCGCTGTTCGCCCGCAAAACCGACAAACGACCGGGAAAAAAACCAAACTGTCATcc > 8:243698/1‑89 (MQ=255) ggCCAGAGGCCCCTTTTTTCATGGGGCATATGTGCGCTGTTCGCCCGCAAAACCGACAAACGACCGGGAAAAAAACCAAACTGTCATCCAGACTTGTGTATCCTGCCCGCGCTTTTTGAAGCGACAAACGCGCCTg > 2:65699/1‑136 (MQ=255) ccAGAGGCCCGGTTTTTGATGGGGCATATTTGCGGGGTTCGCCCGCAAAACCGACAAACGACCGGGAAAAAAACCAAACTGTCATCCAGACTTGTGTATCCTGCCCGAGCTTTTTGAAGCTACAAACGCGCCGGgc > 4:317096/1‑136 (MQ=255) ccAGAGGCCCCTTTTTTTATGGGGCATATGTGCGCAGTTCGCCCGCCAAAACGACAAACGACCGGGAAAAAAACCAAACTGGCATCCAGAAGTGTGGATACTGCCCGAGCGTTTTGATGAGTCAAACGCGCCAGgc > 2:323226/1‑136 (MQ=255) gCCCCGTTTTTCATGGGGCATATGTGCGCGGTTCGCCCGCAGAACCGCCAAACGCCCCGGAAAAAAACCAAACTGTCATCCCGACTTGTTATTCCTGCCCGACCGTTTTGAAGCTACAAAATCGCCGGGCGCAgcc > 8:308857/1‑134 (MQ=255) | CACCGTGACCTGGTGGAGCCACTCGATCAAAGGCCTGCACCGCAACGACTTCATCATGTGTGCACGCACTGACAAGGTGGCGGAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCCTTTTTTCATGGGGCATATGTGCGCTGTTCGCCCGCAAAACCGACAAACGACCGGGAAAAAAACCAAACTGTCATCCAGACTTGTGTATCCTGCCCGAGCTTTTTGAAGCTACAAACGCGCCTGGCGCAGAC > ACB143/5096282‑5096546 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |