Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB143	5,154,737	C→T	5.3%	D62D (GAC→GAT)	CJ019_04852 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB143	5,154,737	0	C	T	5.3%	263.1 / 3.1	76	D62D (GAC→GAT)	CJ019_04852	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (41/31);  new base T (2/2);  total (43/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.44e-01

CTGAGTGCCCCGGCCAGTGCTGCTGTCGACGCCAAGCTGCTCGAAATGCTCCGCGCCAATGGCTCGATCAACCAGGCGCAGTACAACGAACTGCAGGGCGACCTGGCAAAAGAAACCAAGGAAAAGGCCGACCAGAAAGCTCAGTCCGAACGTATGAGCTCCTTTGAACAAAAAGTGGCATGGGCCGCCAAGACCCAGATCAAGGGTGACGTGCGCCTGCGTTACGAAGACGTCAACGTCGACGATCCGAACAGCAGCAGCGGC  >  ACB143/5154606‑5154869                                                                                                                                    |                                                                                                                                     cTGAGTGCCCCGGCCAGTGCTGCTGTCGACGCCAAGCTGCTCGAAATGCTCCGCGCCAATGGCTCGATCAACCAGGCGCAGTACAACGAACTGCAGGGCGACCTGGCAAAAGAAACCAAGGAAAAGGCCGACCAg                                                                                                                                   >  6:355739/1‑135 (MQ=255) cTGAGTGCCCCGGCCAGTGCTGCTGTCGACGCCAAGCTGCTCGAAATGCTCCGCGCCAATGGCTCGATCAACCAGGCGCAGTACAACGAACTGCAGGGCGACCTGGCAAAAGAAACCAAGGAAAAGGCCGACCAg                                                                                                                                   >  6:161994/1‑135 (MQ=255) cTGAGTGCCCCGGCCAGTGCTGCTGTCGACGCCAAGCTGCTCGAAATGCTCCGCGCCAATGGCTCGATCAACCAGGCGCAGTACAACGAACTGCAGGGCGACCTGGCAAAAGAAACCAAGGAAAAGGCCGACCAg                                                                                                                                   >  4:87234/1‑135 (MQ=255)       gCCCCGGCCAGTGCTGCTGTCGACGCCAAGCTGCTCGAAATGCTCCGCGCCAATGGCTCGATCAACCAGGCGCAGTACAACGAACTGCAGGGCGACCTGGCAAAAGAAACCAAGGAAAAGGCCGACCAGAAAGCt                                                                                                                             >  7:286272/1‑135 (MQ=255)        ccccGGCCATTGCTGCTGTCGACTCCAAGCTGCTCGAAATGCTCCGCGCCAATGGCTCGATCAACCAGGCGCAGTACAACGAACTGCAGGGCGACCTGGCAAAAGAAACCAAGGAAAAGGCCGACCAGAAAGCTc                                                                                                                            <  3:117020/135‑1 (MQ=255)        ccccGGCCAGTGCTGCTGTCGACGCCAAGCTGCTCGAAATGCTCCGCGCCAATGGCTCGATCAACCAGGCGCAGTACAACGAACTGCAGGGCGACCTGGCAAAAGAAACCAAGGAAAAGGCCGACCAGAAAGCTc                                                                                                                            <  1:293581/135‑1 (MQ=255)         cccGGCCAGTGCTGCTGTCGACGCCAAGCTGCTCGAAATGCTCCGCGCCAATGGCTCGATCAACCAGGCGCAGTACAACGAACTGCAGGGCGACCTGGCAAAAGAAACCAAGGAAAAGGCCGACCAGAAAGCTCAg                                                                                                                          <  4:348723/136‑1 (MQ=255)          ccGGCCAGTGCTGCTGTCGACGCCAAGCTGCTCGAAATGCTCCGCGCCAATGGCTCGATCAACCAGGCGCAGTACAACGAACTGCAGGGCGACCTGGCAAAAGAAACCAAGGAAAAGGCCGACCAGAAAGCTCAGt                                                                                                                         <  7:275437/136‑1 (MQ=255)           cGGCCAGTGCTGCTGTCGACGCCAAGCTGCTCGAAATGCTCCGCGCCAATGGCTCGATCAACCAGGCGCAGTACAACGAACTGCAGGGCGACCTGGCAAAAGAAACCAAGGAAAAGGCCGACCAGAAAGCTCAGTc                                                                                                                        >  3:306546/1‑136 (MQ=255)            ggCCAGTGCTGCTGTCGACGCCAAGCTGCTCGAAATGCTCCGCGCCAATGGCTCGATCAACCAGGCGCAGTACAACGAACTGCAGGGCGACCTGGCAAAAGAAACCAAGGAAAAGGCCGACCAGAAAGCTCAGTcc                                                                                                                       >  2:321750/1‑136 (MQ=255)              ccAGTGCTGCTGTCGACGCCAAGCTGCTCGAAATGCTCCGCGCCTATGGCTCTATCAACCAGGCGCAGTACAACGATCTGCAGGGAGACCTTGCAAAAGAAACCAAGGAAAAGGCCGACCAGAAAGCTCAGTcc                                                                                                                       <  4:241084/134‑1 (MQ=255)                 gTGCTGCTGTCGACGCCAAGCTGCTCGAAATGCTCCGCGCCAATGGCTCGATCAACCAGGCGCAGTACAACGAACTGCAGGGCGACCTGGCAAAAGAAACCAAGGAAAAGGCCGACCAGAAAGCTCAGTCCGAACg                                                                                                                  >  8:9193/1‑136 (MQ=255)                 gTGCTGCTGTCGACGCCAAGCTGCTCGAAATGCTCCGCGCCAATGGCTCGATCAACCAGGCGCAGTACAACGAACTGCAGGGCGACCTGGCAAAAGAAACCAAGGAAAAGGCCGACCAGAAAGCTCAGTCCGAACg                                                                                                                  <  6:350892/136‑1 (MQ=255)                   gctgctGTCGACGCCAAGCTGCTCGAAATGCTCCGCGCCAATGGCTCGATCAACCAGGCGCAGTACAACGAACTGCAGGGCGACCTGGCTAAAGAAACCAAGGAAAAGGCCGACCAGAAAGCTCAGTCCGAACGTa                                                                                                                >  5:361731/1‑136 (MQ=255)                     tgctgTCGACGCCAAGCTGCTCGAAATGCTCCGCGCCAATGGCTCGATCAACCAGGCGCAGTACAACGAACTGCAGGGCGACCTGGCAAAAGAAACCAAGGAAAAGGCCGACCAGAAAGCTCAGTCCGAACGTAt                                                                                                               >  4:261399/1‑135 (MQ=255)                     tgctgTCGACGCCAAGCTGCTCGAAATGCTCCGCGCCAATGGCTCGATCAACCAGGCGCAGTACAACGAACTGCAGGGCGACCTGGCAAAAGAAACCAAGGAAAAGGCCGACCAGAAAGCTCAGTCCGAACGTATg                                                                                                              >  3:214707/1‑136 (MQ=255)                          tCGACGCCAAGCTGCTCGAAATGCTCCGCGCCAATGGCTCGATCAACCAGGCGCAGTACAACGAACTGCAGGGCGCCCTGGCAAAAGCAACCAAGGAAAAGGCCGACCAGAAAGCTCAGTCCGAACGTATGAGCTc                                                                                                         >  7:180058/1‑136 (MQ=255)                          tCGACGCCAAGCTGCTCGAAATGCTCCGCGCCAATGGCTCGATCAACCAGGCGCAGTACAACGAACTGCAGGGCGACCTGGCAAAAGAAACCAAGGAAAAGGCCGACCAGAAAGCTCAGTCCGAACGTATGAGCTc                                                                                                         >  1:199783/1‑136 (MQ=255)                          tCGACGCCAAGCTGCTCGAAATGCTCCGCGCCAATGGCTCGATCAACCAGGCGCAGTACAACGAACTGCAGGGCGACCTGGCAAAAGAAACCAAGGAAAAGGCCGACCAGAAAGCTCAGTCCGAACGTATGAGCTc                                                                                                         >  6:374565/1‑136 (MQ=255)                          tCGACGCCAAGCTGCTCGAAATGCTCCGCGCCAATGGCTCGATCAACCAGGCGCAGTACAACGAACTGCAGGGCGACCTGGCAAAAGAAACCAAGGAAAAGGCCGACCAGAAAGCTCAGTCCGAACGTATGAGCTc                                                                                                         >  7:99475/1‑136 (MQ=255)                          tCGACGCCAAGCTGCTCGAAATGCTCCGCGCCAATGGCTCGATCAACCAGGCGCAGTACAACGAACTGCAGGGCGACCTGGCAAAAGAAACCAAGGAAAAGGCCGACCAGAAAGCTCAGTCCGAACGTATGAGCTc                                                                                                         >  3:187336/1‑136 (MQ=255)                          tCGACGCCAAGCTGCTCGAAATGCTCCGAGCCAATGGCTCGATCAACCAGGCGAAGTACAAAGAACTCCAGGGCGACCTGGCAAAAGAAACCAAGGAAAAGGCCGACCAGAAAGCTAAGTCCGAACGTATGAGCTc                                                                                                         >  2:243526/1‑136 (MQ=255)                              cGCCAAGCTGCTCGAAATGCTCCGCGCCAATGGCTCGATCAACCAGGCGCAGTACAACGAACTGCAGGGCGACCTGGCAAAAGAAACCAAGGAAAAGGCCGACCAGAAAGCTCAGTCCGAACGTATGAGCTCCttt                                                                                                     >  5:206633/1‑136 (MQ=255)                               gCCAAGCTGCTCGAAATGCTCCGCGCCAATGGCTCGATCAACCAGGCGCAGTACAACGAACTGCAGGGCGACCTGGCAAAAGAAACCAAGGAAAAGGCCGATCAGAAAGCTCAGTCCGAACGTAt                                                                                                               <  2:100159/125‑1 (MQ=255)                               gCCAAGCTGCTCGAAATGCTCCGCGCCAATGGCTCGATCAACCAGGCGCAGTACAACGAACTGCAGGGCGACCTGGCAAAAGAAACCAAGGAAAAGGCCGATCAGAAAGCTCAGTCCGAACGTAt                                                                                                               >  1:105756/1‑125 (MQ=255)                               gCCAAGCTGCTCGAAATGCTCCGCGCCAATGGCTCGATCAACCAGGCGCAGTACAACGAACTGCAGGGCGACCTGGCAAAAGAAACCAAGGAAAAGGCCGATCAGAAAGCTCAGTCCGAACGTAt                                                                                                               <  2:105756/125‑1 (MQ=255)                               gCCAAGCTGCTCGAAATGCTCCGCGCCAATGGCTCGATCAACCAGGCGCAGTACAACGAACTGCAGGGCGACCTGGCAAAAGAAACCAAGGAAAAGGCCGATCAGAAAGCTCAGTCCGAACGTAt                                                                                                               >  1:100159/1‑125 (MQ=255)                               gCCAAGCTGCTCGAAATGCTCCGCGCCAATGGCTCGATCAACCAGGCGCAGTACAACGAACTGCAGGGCGACCTGGCAAAAGAAACCAAGGAAAAGGCCGACCAGAAAGCTCAGTCCGAACGTATGAGCTCCttt                                                                                                     >  4:187911/1‑135 (MQ=255)                                ccAAGCTGCTCGAAATGCTCCGCGCCAATGGCTCGATCAACCAGGCGCAGTACAACGAACTGCAGGGCGACCTGGCAAAAGAAACCAAGGAAAAGGCCGACCAGAAAGCTCAGTCCGAACGTATGAGCTCCTTTGa                                                                                                   <  8:378009/136‑1 (MQ=255)                                ccAAGCTGCTCGAAATGCGCCGCGCCAATGGCTCGATCAACCAGGCGCAGTACAACGAACTGCAGGGCGACCTGGCAAAAGAAACCAAGGAAAAGGCCGACCAGAAAGCTCAGTCCGAACGTATGAGCTCCTTTGa                                                                                                   <  8:21677/136‑1 (MQ=255)                                  aaGCTGCTCGAAATGCTCCGCGCCAATGGCTCGATCAACCAGGCGCAGTACAACGAACTGCAGGGCGACCTGGCAAAAGAAACCAAGGAAAAGGCCGACCAGaaa                                                                                                                                <  5:361195/105‑1 (MQ=255)                                  aaGCTGCTCGAAATGCTCCGCGCCAATGGCTCGATCAACCAGGCGCAGTACAACGAACTGCAGGGCGACCTGGCAAAAGAAACCAAGGAAAAGGCCGACCAGaaa                                                                                                                                >  6:361195/1‑105 (MQ=255)                                  aaGCTGCTCGAAATGCTCCGCGCCAATGGCTCGATCAACCAGGCGCAGTACAACGAACTGCAGGGCGACCTGGCAAAAGAAACCAAGGAAAAGGCCGACCAGAAAGCTCAGTCCGAACGTATGAGCTCCTTTGAAc                                                                                                 <  2:102047/136‑1 (MQ=255)                                           gAAATGCTCCGCGCCAATGGCTCTATCTACCAGGCGCAGTACAACGAACTGCAGGGCGACCTGGCAAAAGAAACCAAGGAAAAGGCCGACCAGAAAGCTCAGTCCGAACGTATGAGCTCCTTTGAACAAAAAGTgg                                                                                        >  2:373651/1‑136 (MQ=255)                                           gAAATGCTCCGCGCCAATGGCTCGATCAACCAGGCGCAGTACAACGAACTGCAGGGCGACCTGGCAAAAGAAACCAAGGAAAAGGCCGACCAGAAAGCTCAGTCCGAACGTATGAGCTCCTTTGAACAAAAAGTgg                                                                                        >  3:320075/1‑136 (MQ=255)                                               tGCTCCGCGCCAATGGCTCGCTCAACCAGGCGCAGTACAACGAACTGCAGGGCGACCTGGCAAAAGAAACCAAGGAAAAGGCCGACCAGAAAGCTCAGTCCGAACGTATGAGCTCCTTTGATCAAAAAGTGGCATg                                                                                    <  4:400199/136‑1 (MQ=255)                                               tGCTCCGCGCCAATGGCTCGATCAACCAGGCGCAGTACAACGAACTGCAGGGCGACCTGGCAAAAGAAACCAAGGAAAAGGCCGACCAGAAAGCTCAGTCCGAACGTATGAGCTCCTTTGAACAAAAAGTGGCATg                                                                                    <  1:283882/136‑1 (MQ=255)                                               tGCTCCGCGCCAATGGCTCGATCAACCAGGCGCAGTACAACGAACTGCAGGGCGACCTGGCAAAAGAAACCAAGGAAAAGGCCGACCAGAAAGCTCAGTCCGAACGTATGAGCTCCTTTGAACAAAAAGTGGCATg                                                                                    <  7:144231/136‑1 (MQ=255)                                                      cgcCAATGGCTCGATCAACCAGGCGCAGTACAACGAACTGCAGGGCGACCTGGCAAAAGAAACCAAGGAAAAGGCCGACCAGAAAGCTCAGTCCGAACGTATGAGCTCCTTTGAACAAAAAGTGGCATGGgccgc                                                                              >  7:140261/1‑135 (MQ=255)                                                         cAATGGCTCGATCAACCAGGCGCAGTACAACGAACTGCAGGGCGACCTGGCAAAAGAAACCAAGGAAAAGGCCGACCAGAAAGCTCAGTCCGAACGTATGAGCTCCTTTGAACAAAAAGTGGCATGGGCCGCCAAg                                                                          <  5:340895/136‑1 (MQ=255)                                                         cAATGGCTCGATCAACCAGGCGCAGTACAACGAACTGCAGGGCGACCTGGCAAAAGAAACCAAGGAAAAGGCCGACCAGAAAGCTCAGTCCGAACGTATGAGCTCCTTTGAACAAAAAGTGGCATGGGCCGCCAAg                                                                          <  6:341800/136‑1 (MQ=255)                                                         cAATGGCTCGATCAACCAGGCGCAGTACAACGAACTGCAGGGCGACCTGGCAAAAGAAACCAAGGAAAAGGCCGACCAGAAAGCTCAGTCCGAACGTATGAGCTCCTTTGAACAAAAAGTGGCATGGGCCGCCAAg                                                                          <  8:340391/136‑1 (MQ=255)                                                            tGGCTCGATCAACCAGGCGCAGTACAACGAACTGCAGGGCGACCTGGCAAAAGAAACCAAGGAAAAGGCCGACCAGAAAGCTCAGTCCGAACGTATGAGCTCCTTTGAACAAAAAGTGGCATGGGCCGCCAAGAcc                                                                       <  2:58191/136‑1 (MQ=255)                                                              gatCGATCAACCAGGCGCAGTACAACGAACTGCAGGGCGAGCTGGCAAAAGTCACCAACTAAAAGGCCGACCAGAAAGCTGAGTCCGAAGGTATGAGCTCATTTGAACAAAAAGTGGCATGGGCCGCCAAGACCCa                                                                     <  8:139034/134‑1 (MQ=255)                                                              gCTCGATCAACCAGGCGCAGTACAACGAACTGCAGGGCGACCTGGCAAAAGAAACCAAGGAAAAGGCCGACCAGTAAGCTCAGTCCGAACGTATGAGCTCCTTTGAACAAAAAGTGGCATGGGCCGCCAAGACCCa                                                                     <  1:147167/136‑1 (MQ=255)                                                              gCTCGATCAACCAGGCGCAGTACAACGAACTGCAGGGCGACCTGGCAAAAGAAACCAAGGAAAAGGCCGACCAGAAAGCTCAGTCCGAACGTATGAGCTCCTTTGAACAAAAAGTGGCATGGGCCGCCAAGACCCa                                                                     <  4:248214/136‑1 (MQ=255)                                                                      aaCCAGGCGCAGTACAACGAACTGCAGGGCGACCTGGCAAAAGAAACCAAGGAAAAGGCCGACCAGAAAGCTCAGTCCGAACGTATGAGCTCCTTTGAACAAAAAGTGGCATGGGCCGCCAAGACCCAGATCAAgg                                                             >  4:177626/1‑136 (MQ=255)                                                                              gcAGTACAACGAACTGCAGGGCGACCTGGCAAAAGAAACCAAGGAAAAGGCCGACCAGAAAGCTCAGTCCGAACGTATGAGCTCCTTTGAACAAAAAGTGGCATGGGCCGCCAAGACCCAGATCAAGGGTGACGTg                                                     <  2:188927/136‑1 (MQ=255)                                                                                aGTACAACGAACTGCAGGGCGACCTGGCAAAAGAAACCAAGGAAAAGGCCGACCAGAAAGCTCAGTCCGAACGTATGAGCTCCTTTGAACAAAAAGTGGCATGGGCCGCCAAGACCCAGATCAAGGGTGACGTgcg                                                   >  7:271/1‑136 (MQ=255)                                                                                aGTACAACGAACTGCAGGGCGACCTGGCAAAAGAAACCAAGGAAAAGGCCGACCAGAAAGCTCAGTCCGAACGTATGAGCTCCTTTGAACAAAAAGTGGCATGGGCCGCCAAGACCCAGATCAAGGGTGACGTgcg                                                   >  7:135656/1‑136 (MQ=255)                                                                                        gAACTGCAGGGCGACCTGGCAAAAGACACCAAGGAAAAGGCCGACCAGAAAGCTCAGTCCGAACGTATGAGCTCCTTTGAACAAAAAGTGGCATGGGCCGCCAAGACCCAGATCAAGGGTGACGTGCGCCTGCGtt                                           <  5:348066/136‑1 (MQ=255)                                                                                          aCTGCAGGGCGACCTGGCAAAAGAAACCAAGGAAAAGGCCGACCAGAAAGCTCAGTCCGAACGTATGAGCTCCTTTGAACAAAAAGTGGCATGGGCCGCCAAGACCCAGATCAAGGGTGACGTGCGCCTGCGTTAc                                         <  7:332019/136‑1 (MQ=255)                                                                                             gCAGGGCGACCTGGCAAAAGAAACCAAGGAAAAGGCCGACCAGAAAGCTCAGTCCGAACGTATGAGCTCCTTTGAACAAAAAGTGGCATGGGCCGCCAAGACCCAGATCAAGGGTGACGTGCGCCTGCGTTACg                                        >  1:388691/1‑134 (MQ=255)                                                                                             gCAGGGCGACCTGGCAAAAGAAACCAAGGAAAAGGCCGACCAGAAAGCTCAGTCCGAACGTATGAGCTCCTTTGAACAAAAAGTGGCATGGGCCGCCAAGACCCAGATCAAGGGTGACGTGCGCCTGCGTTACg                                        >  4:269040/1‑134 (MQ=255)                                                                                               aGGGCGACCTGGCAAAAGAAACCAAGGAAAAGGCCGACCAGAAAGCTCAGTCCGAACGTATGAGCTCCTTTGAACAAAAAGTGGCATGGGCCGCCAAGACCCAGATCAAGGGTGACGTGCGCCTGCGTTACGAAGa                                    >  3:171315/1‑136 (MQ=255)                                                                                               aGGGCGACCTGGCAAAAGAAACCAAGGAAAAGGCCGACCAGAAAGCTCAGTCCGAACGTATGAGCTCCTTTGAACAAAAAGTGGCATGGGCCGCCAAGACCCAGATCAAGGGTGACGTGCGCCTGCGTTACGAAGa                                    >  4:215643/1‑136 (MQ=255)                                                                                               aGGGCGACCTGGCAAAAGAAACCAAGGAAAAGGCCGACCAGAAAGCTCAGTCCGAACGTATGAGCTCCTTTGAACAAAAAGTGGCATGGGCCGCCAAGACCCAGATCAAGGGTGACGTGCGCCTGCGTTACGAAGa                                    >  7:239242/1‑136 (MQ=255)                                                                                               aGGGCGACCTGGCAAAAGAAACCAAGGAAAAGGCCGACCAGAAAGCTCAGTCCGAACGTATGAGCTCCTTTGAACAAAAAGTGGCATGGGCCGCCAAGACCCAGATCAAGGGTGACGTGCGCCTGCGTTACGAAGa                                    >  4:273356/1‑136 (MQ=255)                                                                                               aGGGCGACCTGGCAAAAGAAACCAAGGAAAAGGCCGACCAGAAAGCTCAGTCCGAACGTATGAGCTCCTTTGAACAAAAAGTGGCATGGGCCGCCAAGACCCAGATCAAGGGTGACGTGCGCCTGCGTTACGAAGa                                    >  6:58908/1‑136 (MQ=255)                                                                                               aGGGCGACCTGGCAAAAGAAACCAAGGAAAAGGCCGACCAGAAAGCTCAGTCCGAACGTATGAGCTCCTTTGAACAAAAAGTGGCATGGGCCGCCAAGACCCAGATCAAGGGTGACGTGCGCCTGCGTTACGAAGa                                    >  4:355899/1‑136 (MQ=255)                                                                                                 ggCGACCTGGCAAAAGAAACCAAGGAAAAGGCCGACCAGAAAGCTCAGTCCGAACGTATGAGCTCCTTTGAACAAAAAGTGGCATGGGCCGCCAAGACCCAGATCAAGGGTGACGTGCGCCTGCGTTACGAAGACg                                  >  1:76898/1‑136 (MQ=255)                                                                                                    gACCTGGCAAAAGAAACCAAGGAAAAGGCCGCCCAGAAAGCTCAGTCCGAACGTATGAGCTCCTTTGAACAAAAAGTGGCATGGGCCGCCAAGACCCAGATCAAGGGTGACGTGCGCCTGCGTTACGAAGACGtg                                >  1:80210/1‑134 (MQ=255)                                                                                                      ccTGGCAAAAGAAACCAAGGAAAAGGCCGACCAGAAAGCTCAGTCCGAACGTATGAGCTCCTTTGAACAAAAAGTGGCATGGGCCGCCAAGACCCAGATCAAGGGTGACGTGCGCCTGCGTTACGAAGACGTCAAc                             >  3:16669/1‑136 (MQ=255)                                                                                                      ccTGGCAAAAGAAACCAAGGAAAAGGCCGACCAGAAAGCTCAGTCCGAACGTATGAGCTCCTTTGAACAAAAAGTGGCATGGGCCGCCAAGACCCAGATCAAGGGTGACGTGCGCCTGCGTTACGAAGACGTCAAc                             >  4:85940/1‑136 (MQ=255)                                                                                                       cTGGCAAAAGAAACCAAGGAAAAGGCCGACCAGAAAGCTCAGTCCGAACGTATGAGCTCCTTTGAACAAAAAGTGGCATGGGCCGCCAAGACCCAGATCAAGGGTGACGTGCGCCTGCGTTACGAAGACGTCAACg                            <  5:161994/136‑1 (MQ=255)                                                                                                       cTGGCAAAAGAAACCAAGGAAAAGGCCGACCAGAAAGCTCAGTCCGAACGTATGAGCTCCTTTGAACAAAAAGTGGCATGGGCCGCCAAGACCCAGATCAAGGGTGACGTGCGCCTGCGTTACGAAGACGTCAACg                            <  3:87234/136‑1 (MQ=255)                                                                                                         ggCAAAAGAAACCAAGGAAAAGGCCGACCAGAAAGCTCAGTCCGAACGTATGAGCTCCTTTGAACAAAAAGTGGCATGGGCCGCCAAGCCCCAGATCAAGGGTGACGTGCGCCTGCGTTACGAAGACGTCAACGTc                          >  5:179115/1‑136 (MQ=255)                                                                                                               aGAAACCAAGGAAACGGCCGACCAGAAAGCTCAGTCCGAACGTCTGAGCTCCTTTGAACAAAAAGTGGCAGGGGCCGCCAAGACTCAGATCAAGGGTGACGTGCGCCTGCGTTACGAAGACGTCAACGTCGACGAt                    <  2:301948/136‑1 (MQ=255)                                                                                                               aGAAACCAAGGAAAAGGCCGACCAGAAAGCTCAGTCCGAACGTATGAGCTCCTTTGAACAAAAAGTGGCATGGGCCGCCAAGACCCAGATCAAGGGTGACGTGCGCCTGCGTTACGAAGACGTCAACGTCGACGAt                    <  6:167446/136‑1 (MQ=255)                                                                                                               aGAAACCAAGGAAAAGGCCGACCAGAAAGCTCAGTCCGAACGTATGAGCTCCTTTGAACAAAAAGTGGCATGGGCCGCCAAGACCCAGATCAAGGGTGACGTGCGCCTGCGTTACGAAGACGTCAACGTCGACGAt                    <  7:272938/136‑1 (MQ=255)                                                                                                                 aaaCCAAGGAAAAGGCCGACCAGAAAGCTCAGTCCGAACGTATGAGCTCCTTTGAACAAAAAGTGGCATGGGCCGCCAAGACCCAGATCAAGGGTGACGTGCGCCTGCGTTACGAAGACGTCAACGTCGACGATcc                  <  2:12898/136‑1 (MQ=255)                                                                                                                 aaaCCAAGGAAAAGGCCGACCAGAAAGCTCAGTCCGAACGTATGAGCTCCTTTGAACAAAAAGTGGCATGGGCCGCCAAGACCCAGATCAAGGGTGACGTGCGCCTGCGTTACGAAGACGTCAACGTCGACGATcc                  <  3:188357/136‑1 (MQ=255)                                                                                                                       aGGAAAAGGCCGACCAGAAAGCTCAGTCCGAACGTATGAGCTCCTTTGAACAAAAAGAGGCATGGGCCGCCAAGACCCAGATCAAGGGTGACGTGCGCCTGCGTTACGAAGACGTCAACGTCGACGATCCGAAcag            >  7:161671/1‑136 (MQ=255)                                                                                                                        ggAAAAGGCCGACCAGAAAGCTCAGTCCGAACGTATGAGCTCCTTTGAACAAAAAGTGGCATGGGCCGCCAAGACCCAGATCAAGGGTGACGTGCGCCTGCGTTACGAAGACGTCAACGTCGACGATCCGAAcagc           <  4:384827/136‑1 (MQ=255)                                                                                                                               gCCGACCAGAAAGCTCAGTCCGAACGTATGAGCTCCTTTGAACAAAAAGTGGCATGGGCCGCCAAGACCCAGATCAAGGGTGACGTGCGCCTGCGTTACGAAGACGTCAACGTCGACGATCCGAACAGCAGCAGCg    >  2:243883/1‑136 (MQ=255)                                                                                                                                  gACCAGAAAGCTCAGTCCGAACGTATGAGCTCCTTTGAACAAAAAGTGGCATGGGCCGCCAAGACCCAGATCAAGGGTGACGTGCGCCTGCGTTACGAAGACGTCAACGTCGACGATCCGAACAGCAGCAGCGGc  >  1:397200/1‑135 (MQ=255)                                                                                                                                    |                                                                                                                                     CTGAGTGCCCCGGCCAGTGCTGCTGTCGACGCCAAGCTGCTCGAAATGCTCCGCGCCAATGGCTCGATCAACCAGGCGCAGTACAACGAACTGCAGGGCGACCTGGCAAAAGAAACCAAGGAAAAGGCCGACCAGAAAGCTCAGTCCGAACGTATGAGCTCCTTTGAACAAAAAGTGGCATGGGCCGCCAAGACCCAGATCAAGGGTGACGTGCGCCTGCGTTACGAAGACGTCAACGTCGACGATCCGAACAGCAGCAGCGGC  >  ACB143/5154606‑5154869

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑