| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | ACB143 | 5,386,513 | +AA | intergenic (‑522/+1129) | CJ019_05070 ← / ← nptII | hypothetical protein/neomycin phosphotransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB143 | 5,386,512 | 1 | . | A | 95.0% | 65.6 / ‑2.4 | 20 | intergenic (‑521/+1130) | CJ019_05070/nptII | hypothetical protein/neomycin phosphotransferase |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base A (7/12); total (7/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01 | |||||||||||
| * | ACB143 | 5,386,512 | 2 | . | A | 95.0% | 65.7 / ‑2.4 | 20 | intergenic (‑521/+1130) | CJ019_05070/nptII | hypothetical protein/neomycin phosphotransferase |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base A (7/12); total (7/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.53e-01 | |||||||||||
TTTTTTATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCCGT‑‑CGT‑CA‑GGATC‑‑A‑‑TCCGGGCGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTGGG > ACB143/5386412‑5386605 || ttttttATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTGTACGGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGcc > 1:7438/1‑135 (MQ=255) tAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTGTACGGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGAt < 5:337162/134‑1 (MQ=255) aaTTCATCGAATTCTATCATTTAAGACCCAAAAATGACAACTCGGCCGGCTTGTCGACGACGGCGGTCTCAGTGGAGT‑AC‑GGTAC‑‑AACCCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTcc < 7:343788/135‑1 (MQ=255) ttGATCGAATTCTTTCATTTAACACCCTAATATGCCATCTCGGCCGGCTTGTCGACTACGGCGGTCTCAGTGGGGT‑AC‑GGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCgg < 5:218344/135‑1 (MQ=255) ttGAGCGAATGCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTGTACGGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCgg < 6:244554/135‑1 (MQ=255) tttCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTGTACGGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTa < 2:206841/134‑1 (MQ=255) ttCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTGTACGGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTAtt < 6:334475/135‑1 (MQ=255) tttAAGCCCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTGTACGGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTgcgc < 3:303300/135‑1 (MQ=255) tttAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTGTACGGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTgcgc < 2:310793/135‑1 (MQ=255) tttAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTGTACGGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTgcgc < 5:4928/135‑1 (MQ=255) tatGCCATCTCGGCCGGCTTGTGGACGACGGCGGTCTCAGT‑‑GGTGTACGGTACAAA‑‑CCCAGTCGCTAGCAACGCATGAGAAAGCGCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCTGGTGCCAgg < 8:282573/135‑1 (MQ=255) cATCTCGGCCGGCTTGGCGACGACGGCGGTCTCAGT‑‑GGTGTACGGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTg < 2:30073/135‑1 (MQ=255) ctcGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTGTACGGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTTTTGCGCTGCGGGGGCCAGGGCGTGccc > 5:232818/1‑135 (MQ=255) cggccggcTTGTCGACGACGGCGGTCTCAGT‑‑GGTGTACGGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCtt > 5:119186/1‑135 (MQ=255) ggccggcTTGTCGACGACGGCGGTCTCAGT‑‑GGTGTACGGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTg > 3:104070/1‑135 (MQ=255) gccggcTTGTCGACGACGGCGGTCTCAGT‑‑GGTGTACGGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCtt < 2:18121/133‑1 (MQ=255) gccggcTTGTCGACGACGGCGGTCTCAGT‑‑GGTGTACGGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCtt < 1:176249/133‑1 (MQ=255) gccggcTTGTCGACGACGGCGGTCTCAGT‑‑GGTGTACGGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTgg > 7:104309/1‑135 (MQ=255) ccggcTTGTCGACGACGGCGGTCTCAGT‑‑GGTGTACGGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTACGGGGGCTTTTTTATTGCGCTGCGGGTGCCCGGGCGTGCCCTTggg > 3:328745/1‑135 (MQ=255) ccggcTTGTCGACGACGGCGGTCTCAGT‑‑GGTGTACGGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATAACCTTACGGGGGCTTTTTTATTTCGGTTCGGGTGCCAGGGCGTGCCCGTggg > 3:188200/1‑135 (MQ=255) || TTTTTTATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCCGT‑‑CGT‑CA‑GGATC‑‑A‑‑TCCGGGCGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTGGG > ACB143/5386412‑5386605 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |