Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB143	394,879	0	T	G	39.3%	53.1 / 11.6	62	intergenic (+356/+73)	CJ019_00362/yibF	IS110 family transposase ISPpu11/putative GST‑like protein YibF
Reads supporting (aligned to +/- strand):  ref base T (26/11);  new base G (24/0);  total (51/11)
Fisher's exact test for biased strand distribution p-value = 2.22e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

ACAGTGTGGGCGAGTCCATACATAGACAGTGTGGGCGAGTCCATACATAGGAGCGGGCTTGCCCGCGAACACACATAATGGGGTGTCAGGCACCGGCTTCGCCGGTGTTCGCGGGCACGCCCGCTCCCAAAAGCCCACCACAGATAGCGAACACTGTGGAAAACCTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCCTCAGGCCACTGGCGCTGTAGCGACCATCGATGGCCGCTTGACCACCTCGGCATACCACGCCG  >  ACB143/394755‑395013                                                                                                                             |                                                                                                                                       aCAGGGTGGGCGAGTCCATACATAGACAGTGTGGGCGAGTCCATACATAGGAGCGGGCTTGCCCGCGAACACACATAATGGGGTGTCAGGCACCGGCTTCGCCGGTGTTCGCGGGCACGCCCGCTCCCAAAAGcc                                                                                                                              <  4:70217/135‑1 (MQ=255)      gtgGGCGAGTCCATACATAGACAGTGTGGGCGAGTCCATACATAGGAGCGGGCTTGCCCGCGAACACACATAATGGGGTGTCAGGCACCGGCTTCGCCGGTGTTCGCGGGCACGCCCGCTCCCAAAAGCccacca                                                                                                                         <  1:246794/135‑1 (MQ=255)      gtcggCGAGTCCAAACATAGACAGGGTGGTCGAGTCCATACATAGGCGCGGGCTTGCCCGCGAACACACATAATGGGGTGTCAGGCACCGGCTTCGCCGGTGTTCGCGGGCACGCCCGCTCCCAAAAGCccacca                                                                                                                         <  6:251189/132‑1 (MQ=38)        gggCGAGTCCATACATAGACAGTGTGGGCGAGTCCATACATAGGAGCGGGCTTGCCCGGGAACCCACATAATTGGGTGTCAGGCACCGGCTTCGCCGGTGTTCGCGGGCACGCCCCCGCCCAAAAGCCCACcaca                                                                                                                       >  3:287176/1‑135 (MQ=39)        gggCGAGTCCATACATAGACAGTGTGGGCGAGTCCATACATAGGAGCGGGCTTGCCCGCGAACACACATAATGGGGTGTCAGGCACCGGCTTCGCCGGTGTTCGCGGGCACGCCCGCGCCCAAAAGcccccc                                                                                                                          >  1:83569/1‑132 (MQ=255)        gggCGAGTCCATACATAGACAGTGTGGGCGAGTCCATACATAGGAGCGGGCTTGCCCGCGAACACACATAATGGGGTGTCAGGCACCGGCTTCGCCGGGGTTCGCGGGCCCGCCCGCGCCCAAAAGcccccccaa                                                                                                                       >  7:156968/1‑132 (MQ=38)        gggCGAGTCCATACATAGACAGTGTGGGCGAGTCCATACATAGGAGCGGGCTTGCCCGCGAACACACAAAATGGGGTGTCAGGCACCGGCTTAGCCGGTGTTCGCGGGCCCGCCCGCACCCAAAAGcccccccc                                                                                                                        >  3:292514/1‑132 (MQ=38)          gCGAGTCCATACATAGACAGTGTGGGCGAGTCCATACATAGGAGCGGGCTTGGCCGCGCACACACATAATGGGGTGTCAGGCACCCGCCTCGGCGGGTTTGGCGGGGCCCGCCGCGACCCAAAGCccacccccc                                                                                                                      >  1:144404/1‑130 (MQ=21)               tCCATAATTAGACTGGTTGGGGGAGTCCATACATAGGAGCGGGCTTGCCGGCGAACACACATAATGGGGGGTCAGGCACCGGCTTCGCCGGTGTTCGCGGGCACGCCCGCTCCCAAAAGCCCACCACAGATAGc                                                                                                                 <  6:47381/134‑1 (MQ=37)                ccATACATAGACAGTGTGGGCGAGTCCATACATAGGAGCGGGCTTGCCCGCGAACACACATAATGGGGTGTCAGGCACCGGCTTCGCCGGTGTTCGCGGGCACGCCCGCTCCCAAAAGCCCACCACAGATAGCGa                                                                                                               <  4:147307/135‑1 (MQ=255)                  atacataGACAGTGTGGGCGAGTCCATACATAGGAGCGGGCTTGCCCGCGAACACACATAATGGGGTGTCAGGCACCGGCTTCGCCGGTGTTCGCGGGCACGCCCGCTCCCAAAAGCCCACCACACATAGCGAac                                                                                                             >  8:112970/1‑135 (MQ=255)                  atacataGACAGTGTGGGCGAGTCCATACATAGGAGCGGGCTTGCCCGCGAACACACATAATGGGGTGTCAGGCACCGGCTTAGCCGGTGTTCGCCGGCACCCCCGCGCCCAAAACCCCACCCCACATAGccca                                                                                                              >  3:229612/1‑131 (MQ=37)                  atacataGACAGTGTGGGCGAGTCCATACATAGGAGCGGGCTTGCCCGCGAACACACATAATGGGGTGTCAGGCACCGGCGTAGCCGGTGTTAGCGGGCACCCCCCCGGCCCAAAGCCCACCACCCATAGCGaa                                                                                                              >  3:318996/1‑134 (MQ=37)                           cAGTGTGGGCGAGTCCATACATAGGAGCGGGCTTGCCCGCGAACACACATAATGGGGTGTCAGGCACCGGCTTCGCCGGTGTTCGCGGGCACGCCCGCTCCCCAAAGCCCACCACAGATAGCGAACACTGTGGa                                                                                                     >  2:20598/1‑134 (MQ=255)                           cAGTGTGGGCGAGTCCATACATAGGAGCGGGCTTGCCCGCGAACACACATAATGGGGTGTCAGGCACCGGCTTCGCCGGTGTTAGCGGGCCCCCCCCCTCCCAAAAGCCCACCACACAAAGCGAACACTGTGGa                                                                                                     >  2:336175/1‑134 (MQ=38)                           cAGTGTGGGCGAGTCCATACATAGGAGCGGGCTTGCCCGCGAACACACATAATGGGGTGTCAGGCACCGGCTGCGCCGGTGTTCGCGGGCCCGCCCGCGCGCAAAAGCCCACCCCCGCTAGCGCTCACtgttgg                                                                                                     >  3:219209/1‑131 (MQ=37)                             gtgtGGGCGAGTCCATACATAGGAGCGGGCTTGCCCGCGAACACCCATAATGGGGTGGCAGGCACCCGCTTCGCCGGTGTTCGCGGGCACGCCCGCTCCCAAAAGCCCCCCACCGATAGCGGACACTGGGGaaaa                                                                                                  >  8:78533/1‑135 (MQ=38)                             gtgtGGGCGAGTCCATACATAGGAGCGGGCTTGCCCGCGAACACACATAATGGGGTGTCAGGCACCGGCTTCGCCGGTGTTGGCGGGGCCGCCCCCGCCCAAAAGCCCCCCACCACAAGCGCACACAGTGGaaaa                                                                                                  >  3:177887/1‑135 (MQ=37)                             gtgtGGGCGAGTCCATACATAGGAGCGGGCTTGCCCGCGAACACACATAATGGGGTGTCAGGCACCGGCTTCGCCGGTGTTCGCGGGCCCGCCCGCTCCCAAAAGCCCACCACAGATAGCGAAACCTGTGGaaaa                                                                                                  >  8:70915/1‑135 (MQ=255)                              tgtgGGCGAGTCCATACATAGGAGCGGGCTTGCCCGCGAACACACATAATGGGGTGTCAGGCACCGGCTTCGCCGGTGTTCGCGGGCCCGCCCGCGCCCAAAAGCCCACCACAGATAGCGAACACTGGGGaaaa                                                                                                  >  1:135735/1‑134 (MQ=255)                                 gggCGAGTCCATACATAGGAGCGGGCTTGCCCGCGAACACACATAATGGGGTGTCAGGCACCGGCTTCGCCGGTGTTCGCGGGCCCGCCCGCTCCCAAAAGCCCCCCACCGAAAGCGAACACTTTGGAAAAACtt                                                                                              >  6:63006/1‑135 (MQ=38)                                 gggCGAGTCCATACATAGGAGCGGGCTTGCCCGCGAACACACATAATGGGGTGTCAGGCACCGGCTTCGCCGGTGTTCGCGGGCCCGCCCGCGCCCAAAAGCCCCCCACAGATAGCGCACACTGTGGAAAAccgt                                                                                              >  4:308070/1‑133 (MQ=39)                                 gggCGAGTCCATACATAGGAGCGGGCTTGCCCGCGAACACACATAATGGGGTGTCAGGCACCGGCTTCGCCGGTGTTCGCGGGCACGCCCGCTCCCAAAAGCCCACCCCAGCTAGCGAACACTGTGGAAAACCtt                                                                                              >  3:292879/1‑135 (MQ=255)                                 gggCGAGTCCATACATAGGAGCGGGCTTGCCCGCGAACACACATAATGGGGTGTCAGGCACCGGCTTCGCCGGTGTTAGCGGGGCCCCCCCCGCCCCAAAGCCCACCCCACAATCCCAACACAGTGGCAAAccgt                                                                                              >  3:172338/1‑133 (MQ=255)                                 gggCGAGTCCATACATAGGAGCGGGCTTGCCCGCGAACACACATAATGGGGTGTCAGGCACCGGCTTCGCCGGTGTTAGCGGGCCCCCCCGCGCCCCAAAGCCCACCACAGCTAGCGAACACTGTGGAAAACCtt                                                                                              >  7:156509/1‑135 (MQ=255)                                 gggCGAGTCCATACATAGGAGCGGGCTTGCCCGCGAACACACATAATGGGGTGTCAGGCACCGGCTTCGCCGGTGTTAGCGGGCACGCCCCCTCCCCAAAGCCCACCCCAGCAAGCGAACACTGGGGAAAACCtt                                                                                              >  6:287457/1‑135 (MQ=38)                                 gggCGAGTCCATACATAGGAGCGGGCTTGCCCGCGAACACACATAATGGGGTGTCAGGCACCGGCTTCGCCCGTGTTCGCGGGCACCCCCGCGCCCAAAACCCCAACACAGCAACCGAACCCTGTGGAAAAccgt                                                                                              >  5:185863/1‑133 (MQ=37)                                 gggCGAGTCCATACATAGGAGCGGGCTTGCCCGCGAACACACATAATGGGGTGTCAGGCACCGGCGTCGCCGGTGTTTGCGGGCCCGCCCCCGCCCCAAAGCCCCCCCCCGAGATCGAACACAGTGGGAAAccag                                                                                              >  6:22731/1‑133 (MQ=255)                                 gggCGAGTCCATACATAGGAGCGGGCTTGCCCGCGAACACACATAATGGGGGGTCAGGCACCGGCTTCGCCGGTGTTCGCGGGCACGCCCGCTCCCAAAAGCCCCCCCCACATAGCGAACACAGTGGAAAACCtt                                                                                              >  5:90400/1‑135 (MQ=38)                                   gCGAGTCCATACATAGGAGCGGGCTTTCCCGCGAACACACATAATGGGGTGTCAGGCACCGGCTTCGCCGGTGTTGGCGGGCCCCCCCCCGCCCCAAAGCCCCCCCCCACTAACGAACCCAGTGGTAAACCgtgt                                                                                            >  4:67514/1‑135 (MQ=255)                                   gCGAGTCCATACATAGGAGCGGGCTTGCCCGCGAACCCACATAATGGGGTGTCAGGCACCGGCTTCGCCGGTGTTCGCGGGCCCGCCCGGGCCCCAAAGCCCACCACAGATAGCGAACACTGTGGGAAACCTTGt                                                                                            >  4:292529/1‑135 (MQ=39)                                   gCGAGTCCATACATAGGAGCGGGCTTGCCCGCGAACACACATAATGGGGTGTCAGGCACCGGCTTCGCCGGTGTTCGCGGGCACGCCCGGGCCCCAAAGCCCCCCCCCTAGAGCGAACACAGTGGAAAAccgttg                                                                                            >  4:62368/1‑131 (MQ=37)                                     gAGTCCATACATAGGAGCGGGCTTGCCCGCGAACACACAAAATGGGGTGTCAGGCACCGGCTTCGCCGGTGTTCGCGGGCACGCCCGCTCCCAAAAGCCCACCACAGATAGCGCCCACGGTGGCAAAccgagga                                                                                           >  3:110982/1‑129 (MQ=37)                                         ccATACATAGGAGCGGGCTTGCCCGCGAACCCACAGAATTGGGTGTCAGGCAACGGCTTCGCCGGTGTTCGCCGGCACGCCCGCTCCCAAAAGCCCACCACACATAGCGAACACAGTTGAAAACCATGTATGAGc                                                                                      >  8:288530/1‑135 (MQ=37)                                           atacataGGAGCGGGCTTTCCCGCGAACACAAATAATGTGGTGTCAGGCACCCGCTTAGCCGGTGTTAGCGGGCAAGCCCGCTCCCAAAAGACCAACAAATATATCGAACACATTGGCAAAACTTTTATGAGCgg                                                                                    >  2:198396/1‑135 (MQ=19)                                           atacataGGAGCGGGCTTGCCCGCGAACACACATAATGGGGTGTCAGGCACCGGCTTCGCCGGTGTTCGCGGGCCCGCCCGCTCCCAAAAGCCCACCCCAGCTAGCGAACACTGTGGAAAACCTTGTAGGAGCgg                                                                                    >  7:330300/1‑135 (MQ=255)                                           atacataGGAGCGGGCTTGCCCGCGAACACACATAATGGGGTGTCAGGCACCGGCTTCGCCGGTGTTCGCGGGCACGCCCGCTCCCAAAAGCCCACCACAGATAGCGAACACTGTGGAAAACCTTGTAGGAGCgg                                                                                    >  1:206702/1‑135 (MQ=255)                                           atacataGGAGCGGGCTTGCCCGCGAACACACATAATGGGGTGTCAGGCACCGGCTTCGCCGGTGTTCGCGGGCACGCCCGCTCCCAAAAGCCCACCACAGATAGCGAACACTGTGGAAAACCTTGTAGGAGCgg                                                                                    >  5:254265/1‑135 (MQ=255)                                               ataGGAGCGGGCTTGCCCGCGAACACACATAATGGGGTGTCAGGCACCGGCTTCGCCGGTGTTCGCGGGCACCCCCGCGCCCAAAACCCCACCACAGATAGCGCACCCTGTGGCAAACCATGGAGGCGCGGGtt                                                                                 >  3:7457/1‑134 (MQ=37)                                                    cgCGGGCTTGCCCGCGACCACACATAATGGGGTGTCAGGCACCGGGTTGGCCGGTTTTCGCGGGCACGCCCGCGCCCAAAATCACACCACACATAGCGACAACTTGTGAAAACATTGTAGGAGAGGGgtttaacc                                                                           >  8:330180/2‑130 (MQ=255)                                                      cGGGCTTGCCCGCGAACACACATAATGGGGTGTCAGGCACCGGCTTCGCCGGTGTTAGCGGGGCCCCCCGCGGCCCAAAGCCCCCCCCACAAAGCGAACACAGGGGAAAACCTTGTTGGAGCGGGTTTAccccc                                                                          >  4:179708/1‑132 (MQ=255)                                                           ttGCCCGCGAACACACATAATGGGGTGTCAGGCACCCGCTTCGCCGGTGTTGGCGGGGCCCCCCGCGCCCCAAAGCCCACCACAGATATCGAACACAGTGGAAAAACTTGGAGGAGCCGGTTTTACCGCGAAggg                                                                    >  4:329432/1‑135 (MQ=255)                                                            tGCCCGCGAACACACATAATGGGGTGTCAGGCACCGGCTTCGCCGGTGTTCGCGGGCACGCCCGCGCCCAAAAGCCCACCACAGATAGCGAACACTGTGGAAAACCGTGTAGGAGCGGGTTTAACCGCGAAgggg                                                                   >  3:299168/1‑135 (MQ=255)                                                            tGCCCGCGAAAACACATAATGGGGTGTCAGGCACCGGCTTCGCCGGTGTTCGCCGGCCCGCCCCCGCCCAAAAGCCCACCACACATAGCGAACACTGTGGAAAACCATGTAGGAGCGGGTTTACCCGCGAAcggg                                                                   >  6:238806/1‑135 (MQ=255)                                                              cccGCGAACACACATAATGGGGGGTCAGGCACCGGCTTGGCCGGTGTTCGCGGGCACGCCCGCTCCCAAAAGCCCACCACAGATAGCGAACACTGTGGAAAACCTTGTAGGAGCGGGTTTACCCGCGAAGGGGc                                                                  <  7:292441/134‑1 (MQ=255)                                                                     acacacATAATGGGGTGGCAGGCACCGGCTTCGCCGGTGTTCGCGGGCACGCCCGCTCCCAAAAGCCCACCACAGATAGCGAACACTGTGGAAAACCTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCCTCAGGc                                                          <  2:270929/135‑1 (MQ=255)                                                                          caTAATGGGGGGTCAGGCACCGGCTGCGCCGGTGTTCGCGGGCACGCCCGCTCCCAAAAGCCCACCACAGATAGCGAACACTGTGGAAAACCTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCCTCAGGCCACTg                                                     <  4:295281/135‑1 (MQ=255)                                                                                   gtgtCAGGCACCGGCTTCGCCGGTGTTCGCGGGCACGCCCGCTCCCAAAAGCCCACCACAGATAGCGAACACTGTGGAAAACCTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCCTCAGGCCACTGGCGCTGTAg                                            <  3:174769/135‑1 (MQ=255)                                                                                      tCAGGCACCGGCTTCGCCGGTGTTCGCGGGCACGCCCGCTCCCAAAAGCccacca                                                                                                                         <  4:333349/55‑1 (MQ=255)                                                                                      tCAGGCACCGGCTTCGCCGGTGTTCGCGGGCACGCCCGCTCCCAAAAGCccacca                                                                                                                         >  3:333349/1‑55 (MQ=255)                                                                                      tCAGGCACCGGCTTCGCCGGTGTTCGCGGGCACGCCCGCTCCCAAAAGCCCACCACAGATAGCGAACACTGTGGAAAACCTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCCTCAGGCCACTGGCGCTGTAGCGa                                         >  1:25246/1‑135 (MQ=255)                                                                                      tCAGGCACCGGCTTCGCCGGTGTTCGCGGGCACGCCCGCTCCCAAAAGCCCACCACAGATAGCGAACACTGTGGAAAACCTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCCTCAGGCCACTGGCGCTGTAGCGa                                         >  7:170844/1‑135 (MQ=255)                                                                                      tCAGGCACCGGCTTCGCCGGTGTTCGCGGGCACGCCCGCTCCCAAAAGCCCACCACAGATAGCGAACACTGTGGAAAACCTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCCTCAGGCCACTGGCGCTGTAGCGa                                         >  4:317036/1‑135 (MQ=255)                                                                                        aGGCACCGGCTTCGCCGGTGTTCGCGGGCACGCCCGCTCCCAAAAGCCCACCACAGATAGCGAACACTGTGGAAAACCTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCCTCAGGCCACTGGCGCTGTAGCGAcc                                       >  6:313531/1‑135 (MQ=255)                                                                                        aGGCACCGGCTTCGCCGGTGTTCGCGGGCACGCCCGCTCCCAAAAGCCCACCACAGATAGCGAACACTGTGGAAAACCTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCCTCAGGCCACTGGCGCTGTAGCGAcc                                       >  7:165895/1‑135 (MQ=255)                                                                                        aGGCACCGGCTTCGCCGGTGTTCGCGGGCACGCCCGCTCCCAAAAGCCCACCACAGATAGCGAACACTGTGGAAAACCTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCCTCAGGCCACTGGCGCTGTAGCGAcc                                       >  5:192154/1‑135 (MQ=255)                                                                                        aGGCACCGGCTTCGCCGGTGTTCGCGGGCACGCCCGCTCCCAAAAGCCCACCACAGATAGCGAACACTGTGGAAAACCTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCCTCAGGCCACTGGCGCTGTAGCGAcc                                       >  5:103390/1‑135 (MQ=255)                                                                                        aGGCACCGGCTTCGCCGGTGTTCGCGGGCACGCCCGCGCCCAAAAGCCCCCCCCAGATAGCGCACACAGTGGAAAACCGGGGTGGGGCGGGTTTACCCGCGAACGGGCCCACCGGCCACGGGCGCTGTAGCgcc                                        >  4:147878/1‑132 (MQ=25)                                                                                                   tCGCCGGTGTTCGCGGGCACGCCCGCTCCCAAAAGCCCACCACAGATAGCGAACACTGTGGAAAACCTGGTAGGAGCGGGTTTACCCGCGAAGGGGCCCTCAGGCCACTGGCGCTGGAGCGGCCCTCGATGGCCg                            >  3:191680/1‑135 (MQ=255)                                                                                                     gCCGGTGTTCGCGGGCACGCCCGCTCCCAAAAGCCCACCACAGATAGCGAACACTGTGGAAAACCTTGTAGGAGCGGGGTTACCCGCGAAGGGGCCCTCAGGCCACTGGCGCTGTAGCGACCATCGATGGCCGc                           <  6:90400/134‑1 (MQ=255)                                                                                                             tCGCGGGCACGCCCGCTCCCAAAAGCCCACCACAGATAGCGAACACTGTGGAAAACCTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCCTCAGGCCACTGGCGCTGTAGCGACCATCGATGGCCGCTTGaccacc                  >  6:330312/1‑135 (MQ=255)                                                                                                                 gggCACGCCCGCTCCCAAAAGCCCACCACAGATAGCGAACACTGTGGAAAACCTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCCTCAGGCCACTGGCGCTGTAGCGACCATCGATGGCCGCTTGACCACCTCg               >  7:349649/1‑134 (MQ=255)                                                                                                                             tCCCAAAAGCCCACCACAGATAGCGAACACTGTGGAAAACCTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCCTCAGGCCACTGGCGCTGTAGCGACCATCGATGGCCGCTTGACCACCTCGGCATACCAcgccg  <  8:347960/135‑1 (MQ=255)                                                                                                                             |                                                                                                                                       ACAGTGTGGGCGAGTCCATACATAGACAGTGTGGGCGAGTCCATACATAGGAGCGGGCTTGCCCGCGAACACACATAATGGGGTGTCAGGCACCGGCTTCGCCGGTGTTCGCGGGCACGCCCGCTCCCAAAAGCCCACCACAGATAGCGAACACTGTGGAAAACCTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCCTCAGGCCACTGGCGCTGTAGCGACCATCGATGGCCGCTTGACCACCTCGGCATACCACGCCG  >  ACB143/394755‑395013

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑