Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1434,805,2340AT28.8% 58.5 / 11.4 55intergenic (+161/‑588)dsbG/CJ019_04513Thiol:disulfide interchange protein DsbG/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (4/33);  new base T (8/7);  total (14/41)
Fisher's exact test for biased strand distribution p-value = 2.26e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GCCGTGAAGCAGGCGACGTGGGGCATGGCACCGGCTACGCCGGTGTTCGCGGGTGAACCCGCTCCTACGGGGCTCGCGCTAACGGCTGGGTTTTGCGCCAGGCCCGTGATCGCAAGGCCTGCGCGGTCGGCCGTAGGAGC‑‑GGGTTTACCCGCGAAAGGGCCGGACCTGCTGGCCAATTTCCACCAGCCGTTCACCGTTGCCGCCGCCACTGTATTCGCGGGTAAACCCGCTCCTACGCTGATCGGGGGTGGAGACACATTGGTGATTGG  >  ACB143/4805100‑4805368
                                                                                                                                      |                                                                                                                                            
gcCGTGAAGCAGGCGACGTGGGGCATGGCACCGGCTACGCCGGTGTTCGCGGGTGAACCCGCTCCTACGGGGCTCGCGCTAACGGCTGGGTTTTGCGCCAGGCCCGTGATCGCAAGGCCTGCGCGGTCGGCCGTa                                                                                                                                              <  1:166045/135‑1 (MQ=255)
gcCGTGAAGCAGGCGACGTGGGGCATGGCACCGGCTACGCCGGTGTTCGCGGGTGAACCCGCTCCTACGGGGCTCGCGCTAACGGCTGGGTTTTGCGCCAGGCCCGTGATCGCAAGGCCTGCGCGGTCGGCCGTa                                                                                                                                              >  6:309550/1‑135 (MQ=255)
gcCGTGAAGCAGGCGACGTGGGGCATGGCACCGGCTACGCCGGTGTTCGCGGGTGAACCCGCTCCTACGGGGCTCGCGCTAACGGCTGGGTTTTGCGCCAGGCCCGTGATCGCAAGGCCTGCGCGGTCGGCCGTa                                                                                                                                              <  4:135308/135‑1 (MQ=255)
gcCGTGAAGCAGGCGACGTGGGGCATGGCACCGGCTACGCCGGTGGTCGCGGGTGAACCCGCTCCTACGGGGCTCGCGCTAACGGCTGGGTTTTGCGCCAGGCCCGTGATCGCAAGGCCTGCGCGGTCGGCCGTa                                                                                                                                              <  6:327475/135‑1 (MQ=255)
gcCGGGAAGCAGGCGACGTGGGGCATGGCACCGGCTACGCCGGTGTTCGCGGGTGAACCCGCTCCTACGGGGCTCGCGCTAACGGCTGGGTTTTGCGCCAGGCCCGTGATCGCAAGGCCTGCGCGGTCGGCCGTa                                                                                                                                              <  7:106193/135‑1 (MQ=255)
gcCGGGAAGCAGGCGACGTGGGGCATGGCACCGGCTACGCCGGTGTTCGCGGGTGAACCCGCTCCTACGGGGCTCGCGCTAACGGCTGGGTTTTGCGCCAGGCCCGTGATCGCAAGGCCTGCGCGGTCGGCCGTa                                                                                                                                              <  6:128218/135‑1 (MQ=255)
 ccGTGAAGCCGGCGAGGTGTGGCAGGTCACCGGCTACGCCGGTGTTCGCGGGTGAACCCGCTCCTACGGGGCTCTCGCTATCTGCTTTGTTTTTCTCCATGACCTTTATCGCAAGGCCTGCGCGGTCGGCCGAAg                                                                                                                                             <  8:347509/135‑1 (MQ=21)
  cgggacgCAGGCGACGTGGGGCATGGCACCGGCTACGCCGGTGTTCGCGGGTGAACCCGCTCCTACGGGGCTCGCGCTAACGGCTGGGTTTTGCGCCAGGCCCGTGATCGCAAGGCCTGCGCGGTCGGCCGTAgg                                                                                                                                            <  6:270004/129‑1 (MQ=255)
       aGCAGGCGACGTGGGGCATGGCACCGGCTACGCCGGTGTTCGCGGGTGAACCCGCTCCTACGGGGCTCGCGCTAACGGCTGGGTTTTGCGCCAGGCCCGTGATCGCAAGGCCTGCGCGGTCGGCCGTAGGTGC‑‑gg                                                                                                                                   >  5:265710/1‑135 (MQ=255)
               aCGTGGGGCATGGCACCGGCTACGCCGGTGTTCGCGGGTGAACCCGCTCCTACGGGGCTCGCGCTAACGGCTGGGTTTTGCGCCAGGCCCGTGATCGCAAGGCCTGCGCGGTCGGCCGTAGGGGA‑‑GGGgtttaac                                                                                                                           >  8:327327/1‑131 (MQ=255)
                            cACCGGCTACGCCGGTGTTCGCGGGTGAACCCGCTCCTACGGGGCTCGCGCTAACGGCTGGGTTTTGCGCCAGGCCCGTGATCGCAAGGCCTGCGCGGTCGGCCGTTGGGGCGGGGGTTACCCCGCAAAGGGCCg                                                                                                              >  7:49677/1‑135 (MQ=38)
                             aCCGGCTACGCCGGTGTTCGCGGGTGAACCCGCTCCTACGGGGCTCGCGCTAACGGCTGGGTTTTGCGCCAGGCCCGTGATCGCAAGGCCTGCGCGGTCGGCCGTTGGGGCC‑‑GGGTTTACCCCCGCAAGGGCCgg                                                                                                             >  4:171815/1‑135 (MQ=38)
                              ccGGCTACGCCGGTGTTCGCGGGGCACCCCGCTCCTACGGGGCGCGCGCTACCGGCGGGGTTTTGCGCCAGGCCCGTGATCGCAAGGCCTGCGCGGGCGGCCGTAGGCGC‑‑GGGTTTACCCGCGAAAGGGCCGGAc                                                                                                           <  4:251745/135‑1 (MQ=38)
                                  cTACGCCGGTGTTCGCGGGTGAACCCGCTCCTACGGGGCTCGCGCTAACGGCTGGGTTTTGCGCCAGGCCCGTGATCGCAAGGCCTGCGCGGTCGGCCCTTGGCGGC‑‑GGGTTTCCCCCCCAAAGGGCCgcccagc                                                                                                        >  5:142472/1‑129 (MQ=37)
                                    aCGCCGGTGTTCGCGGGTGAACCCGCTCCTACGGGGCTCGCGCTAACGGCTGGGTTTTGCGCCAGGCCCGTGATCGCAAGGCCTGCGCGGTCGGCCCTTGGGGCGGGGGTTAACCCGCCAAGGGCCGGccccggg                                                                                                      >  4:283929/1‑131 (MQ=37)
                                              tCGCGGGTGAACCCGCTCCTACGGGGCTCGCGCTAACGGCTGGGTTTTGCGCCAGGCCCGTGATCGCACGGCCTGCGCGGTCGGCCGTGGGGGCC‑‑GGGTTTACCCCGCGAAGGGCCGCCCCGGCGGGCCAAttag                                                                                            >  5:172058/1‑133 (MQ=255)
                                              tCGCGGGTGAACCCGCTCCTACGGGGCTCGCGCTAACGGCTGGGTTTTGCGCCAGGCCCGTGATCGCAAGGCCTGCGCGGTCGGCCGTTGGGGCGGGGTTTACCCCGGAAGGGGCGGGCCGGGGGGGCCAAttgc                                                                                            >  5:147501/1‑133 (MQ=255)
                                              tCGCGGGTGAACCCGCTCCTACGGGGCTCGCGCTAACGGCTGGGTTTTGCGCCAGGCCCGTGATCGCAAGGCCTGCGCGGTCGGCCGTTGGAGC‑‑GGGGTTAACCGCGAAATGGCCGGACCTGCGGGCCCAtttac                                                                                           >  6:171858/1‑133 (MQ=255)
                                                              gcctcgggggCTCGCGCTACCGGCTGGGTTTTGCGCCAGGCCCGTGATCGCAAGGCCGGCGGGGTCGGCGGTAGGAGC‑‑GGGTTTACCCGCGAAAGGGCCGGACCTGCTGGCCAATTTCCACCAGCCGTTCACCGt                                                                          <  5:102909/129‑1 (MQ=25)
                                                                 tACGGGGCTCGCGCTAACGGCTGGGTTTTGCGCCAGGCCCGTGATCGCAAGGCCTGCGCGGTCGGCCGTGGGGGG‑‑GGGGTTGCCCCCGCAATGGGCGGCCCTGGTGGGCCATTTCCACCCGCCGGTGACCGGTgg                                                                       >  6:328567/1‑134 (MQ=255)
                                                                   cGGGGCGCCGGCGAAGGGCTGGGTTATGCGCAAGGCCCTGTATCGCACGGCCTGCGCGTTCGGCCGTAGCTGC‑‑GGGTTTACCCGCGAAAGGGCCGGACCTGCTGGCCAATTTCCACCAGCCGTTCACCGTTg                                                                        <  8:173723/132‑1 (MQ=17)
                                                                    ggggCTCGCGCTACGGGCTGGGTTTGGCGCCAGGCCGGTGATCGCACGGCCGGCGCGGTCGGCCGTAGGAGG‑‑GGGTTTACCCGCGAAAGGGCCGGACCTGCTGGCCAATTTCCACCAGCCGTTCACCGTTgccgc                                                                    <  2:249942/135‑1 (MQ=21)
                                                                    ggggCTCGCGCTAACGGCTGGGTTTTCCGCCAGGCCCGTCTACCCACGGCGGGGGGGGTCGGCCGTTGTAGG‑‑GGGTTTACCCGCGAAAGGGCCGGACCTGCTGGCCAATTTCCACCAGCCGTTCACCGTTgccgc                                                                    <  3:46567/135‑1 (MQ=18)
                                                                    ggggCGCGCGCTAACGGGGGGGTTTTGCGCCAGGCCCGTGATCGCACGGCCGGGGCGGGCGGCCGTAGGAGC‑‑GGGTTAACCCGCGAAAGGGCCGGACCTGCTGGCCAATTTCCACCAGCCGTTCACCGTTgccgc                                                                    <  3:232299/135‑1 (MQ=21)
                                                                     gggCTCGCGCTAACGGCTGGGTTTTGCGCCAGGCCCGTGATCGCAAGGCCTGCGCGGTCGGCCGTAGGAGC‑‑GGGTTTACCCGCGAAAGGGCCGGACCTGCTGGCCAATTTCCACCAGCCGTTCACCGTTgc                                                                       >  2:135278/1‑131 (MQ=255)
                                                                     gggCTCGCGCTAACGGCTGGGTTTTGCGCCAGGCCCGTGATCGCAAGGCCTGCGCGGTCGGCCGTAGGAGC‑‑GGGTTTACCCGCGAAAGGGCCGGACCTGCTGGCCAATTTCCACCAGCCGTTCACCGTTgc                                                                       <  1:135278/131‑1 (MQ=255)
                                                                      ggCTCGGGCTAAGGTCTGGTTTTTGGCCCTGGCCCGTGTTCGCATGGGCTGCGCGGTCGGCCTTAGGAGC‑‑GGTTTTACCCGCGAAAGGGCCGGACCTGCTGGCCAATTTCCACCAGCCGTTCACCGTTgccgccg                                                                  <  2:6955/135‑1 (MQ=17)
                                                                      ggCTCGCGCTAACGGCTGGGTTTTGCGCCAGGCCCGTGATCGCAAGGCCTGCGCGGTCGGCCGTAGGAGC‑‑GGGTTTACCCGCGAAAGGGCCGGACCTGCTGGCCAATTTCCACCAGCCGTTCACCGTTgccgccg                                                                  <  2:282389/135‑1 (MQ=255)
                                                                      gccTCGCGCAAACGGCGGGGTTTTGCGCCAGGCCCGTGAACGCACGGCGTGCGCGGGCGGCCGTAGGAGC‑‑GGGTTTACCCGCGAAAGGGCCGGACCTGCTGGCCAATTTCCACCAGCCGTTCACCGTTgccgccg                                                                  <  5:307438/133‑1 (MQ=21)
                                                                           gcgcTAACGGCTGGGTTTTGCGCCAGGCCCGTGATCGCAAGGCCTGCGCGGTCGGCCGTTGGGGCGGGGTTTAACCCCGAAGGGGCGGGCCGGCGGGGCCATTTACACCACCCGGTGACCGGTTgccccccccca                                                              >  2:286299/1‑133 (MQ=255)
                                                                             gcTAACGGCTGGGTTTTGCGCCAGGCCCGTGATCGCAAGGCCTGCGCGGTCGGCCGTTGGGGC‑‑GGGGTTTCCCCCCCAAGGGCCGGCCCAGGGGGCCAAATTCCCCCCCCCGGTGACCCGTGCCCCCGCCCCTgg                                                           >  1:222111/1‑134 (MQ=255)
                                                                               tAACGGGGGGGTTTGGCCCCAGCCCCGTCACCCCACGCCCTGCGGGGGCGGCCGATGGAGC‑‑GGGTTTACCCGCGAAAGGGCCGGACCTGCTGGCCAATTTCCACCAGCCGTTCACCGTTGCCGCCGCCACTGTAt                                                         <  1:358082/135‑1 (MQ=17)
                                                                               aaagggCGGGGTTTTGCGCCCGGCCCGTTATCGCACGGCCGGCGGGGGCGGCCGTAGGAGG‑‑GGGTTTACCCGCGAAAGGGCCGGACCTGCTGGCCAATTTCCACCAGCCGTTCACCGTTGCCGCCGCCACTGTAt                                                         <  1:273826/131‑1 (MQ=18)
                                                                                    gcgggtttttGCGCCAGGCCCGTGATCGCACGGCCGGCGCGGTCGGCCTTAGGAGC‑‑GGGTTTACCCGCGAAAGGGCCGGACCTGCTGGCCAATTTCCACCAGCCGTTCACCGTTGCCGCCGCCACTGTATTcgcg                                                    <  8:171149/129‑1 (MQ=18)
                                                                                    gcggggTTTTGCGCCCGGCCCGTTAGCGCACGGCGTGCGCGGCCGGCGGTTGTAGG‑‑GGTTTTACCCGCGAAAGGGCCGGACCTGCTGGCCAATTTCCACCAGCCGTTCACCGTTGCCGCCGCCACTGTATTcgcg                                                    <  3:98345/132‑1 (MQ=18)
                                                                                         ttttgGCGCCTGGCCGGTGATCCCATGCCCGGCGGGGTGGGCGTTAGGCGG‑‑GGTTTTCCCCGCGAAAGGGCCGGACCTGCTGGCCAATTTCCACCAGCCGTTCACCGTTGCCGCCGCCACTGTATTCGCGGGTaa                                               <  7:336652/134‑1 (MQ=17)
                                                                                         gTTTTGCGCCAGGCCGGGTATCGCATGGCCGGCGGGGGCGGCCGTAGGAGG‑‑GGGTTAACCCGCGAAAGGGCCGGACCTGCTGGCCAATTTCCACCAGCCGTTCACCGTTGCCGCCGCCACTGTATTCGCGGGTaa                                               <  7:1310/135‑1 (MQ=17)
                                                                                         gTTTTGCGCCAGGCCCGTGATCGCAAGGCCTGCGCGGGCGGCCGTAGGAGC‑‑GGGTTTACCCGCGAAAGGGCCGGACCTGCTGGCCAATTTCCACCAGCCGTTCACCGTTGCCGCCGCCACTGTATTCGCGGGTaa                                               <  1:55060/135‑1 (MQ=255)
                                                                                         gTTTTGCCCCCGCCCGGTTACCGCACGGCCGGCGGGGTCGGCGGTAGTAGG‑‑GGTTTAACCCGCGAAAGGGCCGGACCTGCTGGCCAATTTCCACCAGCCGTTCACCGTTGCCGCCGCCACTGTATTCGCGGGTaa                                               <  3:147940/135‑1 (MQ=14)
                                                                                         gTTTTGCCCCAGGCCCGTTATCGCCCGGCGGGGGGGGTCGCCGGTTGGCGG‑‑GGTTTTACCCGCGAAAGGGCCGGACCTGCTGGCCAATTTCCACCAGCCGTTCACCGTTGCCGCCGCCACTGTATTCGCGGGTaa                                               <  8:69753/135‑1 (MQ=14)
                                                                                         gTTTTGCCCCAGGCCCGTGATGGAAGGCCCTGCGCGGTCGGCGGTTGGTCG‑‑GGTTTTACCCGCGAAAGGGCCGGACCTGCTGGCCAATTTCCACCAGCCGTTCACCGTTGCCGCCGCCACTGTATTCGCGGGTaa                                               <  8:228792/135‑1 (MQ=17)
                                                                                         gTTTTCCCCCTGCCCCGTGATCGCAACGCCTGCCGGGTGGGCCTTAGGCGC‑‑GGGTTAACCCGCGAAAGGGCCGGACCTGCTGGCCAATTTCCACCAGCCGTTCACCGTTGCCGCCGCCACTGTATTCGCGGGTaa                                               <  2:248835/135‑1 (MQ=17)
                                                                                         gTTTGGCCCCAGGCCGGTTATCGCACGGCCTGCGGGGGCGCCCGTAGGCGG‑‑GGGTTTACCCGCGAAAGGGCCGGACCTGCTGGCCAATTTCCACCAGCCGTTCACCGTTGCCGCCGCCACTGTATTCGCGGGTaa                                               <  3:37961/135‑1 (MQ=14)
                                                                                            ttGCGCCAGGCCGGTGATCGCAAGGCCTGGGGGGGCGGCCTTAGGAGC‑‑GGTTTAACCCGCGAAAGGGCCGGACCTGCTGGCCAATTTCCACCAGCCGTTCACCGTTGCCGCCGCCACTGTATTCGCGGGTAAAc                                             <  1:240293/134‑1 (MQ=18)
                                                                                            tggcgcCCGGCCGGTTATCCCATGCCCGGCGCGGGCGGCCTTAGGCGG‑‑GGGTTTACCCGCGAAAGGGCCGGACCTGCTGGCCAATTTCCACCAGCCGTTCACCGTTGCCGCCGCCACTGTATTCGCGGGTAAAc                                             <  1:321501/132‑1 (MQ=14)
                                                                                                   aGGCCCGTGATCGCACGGCCTGCGCGGGCGGCCGTAGGAGC‑‑GGGTTTACCCGCGAAAGGGCCGGACCTGCTGGCCAATTTCCACCAGCCGTTCACCGTTGCCGCCGCCACTGTATTCGCGGGTAAACCCGCTCCt                                     <  2:289461/135‑1 (MQ=255)
                                                                                                        ggttagcGCAGGGCGGGCGCGGGCGGCCGTCGGAGC‑‑GGGTTTACCCGCGAAAGGGCCGGACCTGCTGGCCAATTTCCACCAGCCGTTCACCGTTGCCGCCGCCACTGTATTCGCGGGTAAACCCGCTCCTACGCt                                <  7:269621/129‑1 (MQ=14)
                                                                                                        cgttaccGCACGGCCGGGGCGGGCGGCGTTAGTAGC‑‑GGGTTTACCCGCGAAAGGGCCGGACCTGCTGGCCAATTTCCACCAGCCGTTCACCGTTGCCGCCGCCACTGTATTCGCGGGTAAACCCGCTCCTACGCt                                <  3:358991/129‑1 (MQ=14)
                                                                                                        cGTGATCCCATGGCGTGGGCGGGCGGCCGTTGGTGG‑‑GGGTTTACCCGCGAAAGGGCCGGACCTGCTGGCCAATTTCCACCAGCCGTTCACCGTTGCCGCCGCCACTGTATTCGCGGGTAAACCCGCTCCTACGCt                                <  7:335522/135‑1 (MQ=17)
                                                                                                          ttaTCGCAAGGCCTGCGCGGTCGGCCTTAGGAGG‑‑GGGTTTACCCGCGAAAGGGCCGGACCTGCTGGCCAATTTCCACCAGCCGTTCACCGTTGCCGCCGCCACTGTATTCGCGGGTAAACCCGCTCCTACGCTGa                              <  8:223498/133‑1 (MQ=255)
                                                                                                          tGATCGCAAGGCCTGCGCGGTCGGCCTTAGGAGC‑‑GGGTTTACCCGCGAAAGGGCCGGACCTGCTGGCCAATTTCCACCAGCCGTTCACCGTTGCCGCCGCCACTGTATTCGCGGGTAAACCCGCTCCTACGCTGa                              <  1:217818/135‑1 (MQ=255)
                                                                                                          tGATCCCACGGCGTGCGGGGGCGGCCGTAGGCGG‑‑GGTTTAACCCGCGAAAGGGCCGGACCTGCTGGCCAATTTCCACCAGCCGTTCACCGTTGCCGCCGCCACTGTATTCGCGGGTAAACCCGCTCCTACGCTGa                              <  3:80108/135‑1 (MQ=17)
                                                                                                                 atggCCTGCGCGGGCGGCCGTAGGAGG‑‑GGGTTTACCCGCGAAAGGGCCGGACCTGCTGGCCAATTTCCACCAGCCGTTCACCGTTGCCGCCGCCACTGTATTCGCGGGTAAACCCGCTCCTACGCTGATCggggg                       <  6:82298/133‑1 (MQ=255)
                                                                                                                   ggCCTGCGCGGTCGGCCGTAGGAGC‑‑GGGTTTACCCGCGAAAGGGCCGGACCTGCTGGCCAATTTCCACCAGCCGTTCACCGTTGCCGCCGCCACTGTa                                                          <  5:257820/98‑1 (MQ=255)
                                                                                                                   ggCCTGCGCGGTCGGCCGTAGGAGC‑‑GGGTTTACCCGCGAAAGGGCCGGACCTGCTGGCCAATTTCCACCAGCCGTTCACCGTTGCCGCCGCCACTGTa                                                          >  6:257820/1‑98 (MQ=255)
                                                                                                                   gccgtGCGCGGTCGGCCGTAGTAGC‑‑GGGTTTACCCGCGAAAGGGCCGGACCTGCTGGCCAATTTCCACCAGCCGTTCACCGTTGCCGCCGCCACTGTATTCGCGGGTAAACCCGCTCCTACGCTGATCGGGGGTg                     <  1:258390/131‑1 (MQ=255)
                                                                                                                    gcgtGCGCGGGCGGCCGTAGCAGC‑‑GGGTTTACCCGCGATAGGGCCGGACCTGCTGGCCAATTTCCACCAGCCGTTCACCGTTGCCGCCGCCACTGTATTCGCGGGTAAACCCGCTCCTACGCTGATCGGGGGTgg                    <  5:152337/132‑1 (MQ=255)
                                                                                                                    gCCTGCGGGGCGGGCCGTTGGCGC‑‑GGGTTTACCCGCGAAAGGGCCGGACCTGCTGGCCAATTTCCACCAGCCGTTCACCGTTGCCGCCGCCACTGTATTCGCGGGTAAACCCGCTCCTACGCTGATCGGGGGTgg                    <  3:247989/135‑1 (MQ=17)
                                                                                                                    gCCGGCGCGGTCGGCCGTAGGTGC‑‑GGGTTTACCCGCGAAAGGGCCGGACCTGCTGGCCAATTTCCACCAGCCGTTCACCGTTGCCGCCGCCACTGTATTCGCGAGTAAACCCGCTCCTACGCTGATCGGGGGTgg                    <  2:306446/135‑1 (MQ=255)
                                                                                                                       tGCGCGGGCGGCCGTAGGAGC‑‑GGGTTTACCCGCGAAAGGGCCGGACCTGCTGGCCAATTTCCACCAGCCGTTCACCGTTGCCGCCGCCACTGTATTCGCGGGTAAACCCGCTCCTACGCTGATCGGGGGTGgaga                 <  8:38726/135‑1 (MQ=255)
                                                                                                                                ggCGGTAGGTGC‑‑GGGTTTACCCGCGAAAGGGCCGGACCTGCTGGCCAATTTCCACCAGCCGTTCACCGTTGCCGCCGCCACTGTATTCGCGGGTAAACCCGCTCCTACGCTGATCGGGGGTGGAGACACATTGGt        <  3:282399/135‑1 (MQ=255)
                                                                                                                                      aGGAGC‑‑GGGTTTACCCGCGAAAGGGCCGGACCTGCTGGCCAATTTCCACCAGCCGTTCACCGTTGCCGCCGCCACTGTATTCGCGGGTAAACCCGCTCCTACGCTGATCGGGGGGGGAGACACATTGGTGATTgg  <  1:290415/135‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                            
GCCGTGAAGCAGGCGACGTGGGGCATGGCACCGGCTACGCCGGTGTTCGCGGGTGAACCCGCTCCTACGGGGCTCGCGCTAACGGCTGGGTTTTGCGCCAGGCCCGTGATCGCAAGGCCTGCGCGGTCGGCCGTAGGAGC‑‑GGGTTTACCCGCGAAAGGGCCGGACCTGCTGGCCAATTTCCACCAGCCGTTCACCGTTGCCGCCGCCACTGTATTCGCGGGTAAACCCGCTCCTACGCTGATCGGGGGTGGAGACACATTGGTGATTGG  >  ACB143/4805100‑4805368

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: