Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1434,927,1850AC52.0% ‑2.6 / 10.5 27W194G (TGG→GGG) srp54Signal recognition particle 54 kDa protein
Reads supporting (aligned to +/- strand):  ref base A (8/4);  new base C (3/10);  total (11/16)
Fisher's exact test for biased strand distribution p-value = 4.72e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.04e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

AGCTTGGCCAGGGTGGTGGTCTTGCCCATGCCCGCCGGGCCGACCATGGCGATCACCCCACCCTCTTCGATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCAGGCCTGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCGGGCGAT‑CGGCCCGGACAGGCCGATGCGCTGCAGGCGGCGCCAAAGGTTGGCCTGCTGCGGCTTGCTGCCCTGCA  >  ACB143/4927058‑4927307
                                                                                                                               |                                                                                                                           
aGCTTGGCCAGGGTGGTGGTCTTGCCCATGCCCGCCGGGCCGACCATGGCGATCACCCCACCCTCTTCGATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCAGGCCTgg                                                                                                                      >  2:276986/1‑135 (MQ=255)
            gggggggtCTTCCCCATGCCCGCCGGGCCGACCATGGCGATCACCCCACCCTCTTCGAGCGGCTCTATTTCAGGCACTTCCATCATCCGTCCCAAGTGAGCCAGCAGCATGCGCCAGCCCTGGCGCGGCTCTTc                                                                                                           <  6:74670/129‑1 (MQ=255)
              ggtggtCTTGACCATGCACGCCGGGCCGACCATGGCGATCACCCCCCCCTCTTCGATCGGCTCGATTTCAGGCACTTCGATCATACGTGACAAGTGAGCCAGCAGCATGCCCCCAGCCTGGCGCCGccctgggct                                                                                                        >  6:48057/1‑130 (MQ=255)
                        cccATGCCCGCGGGGCCGACCTGGGCTATCACCCCACCCTGTTCGATCGGCTCTATTTCAGGCACTTCGAACATCCTTGCCAAGTGAGCCTGCAGAATGCCCCAGGCGTGGCGCGGCTCTTCGATCTcggcg                                                                                                 <  6:58074/132‑1 (MQ=255)
                        cccATGCCCGCCGGGCCGACCATGGCGATCACCCCACCCTCTTCGATCGGCTCGATTTCAGGCACTTAGATCATCCGTGCAAAGTGAGCCAGCAGCATGCGCCAGGGCTGGAGCGGCTCGTAGATCTgggagg                                                                                                >  4:271928/1‑133 (MQ=255)
                        cccAGGCCCGCCGGGCCGACCTTGGCGACCCCCCCCCCCTCTTCTATCGGCGCGATTTCAGCCACTTCGATCAGCCGTCCCAAGGGACCCAGCAGCAGGCGCCTGGCCGGGCGCGGCTCTTCGATCTcggcg                                                                                                 <  6:259368/132‑1 (MQ=255)
                               ccgccgGGCCGACCATGGCGATCACCCCACCCTCTTCGATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCAGGCCTGGCGCGGGACGTCGCTCTCGGCGGTGAggggca                                                                                       >  3:200725/1‑131 (MQ=255)
                                cgccgGGCCGACCATGGCGATCACCCCACCCTCTTCGATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCCCGCCAGGGGCGGCTCGTCGGTGTGGGCGGGCGGGTGcag                                                                                      >  5:18982/1‑135 (MQ=255)
                                cgccgGGCCGACCATGGCGATCACCCCACCCTCTTCGATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCAGGCCTGGCGCGGCGCGTCGATCTCGGCGGTCAGGTCca                                                                                       >  1:246418/1‑134 (MQ=255)
                                            cATGGCGACCCCCCCCCCCTCTGCGATCGGCTCTATTCCAGCCACTTCTATCATCCGTCCCAAGTGCCCCCGCACCAGGCGCCCGGCCGGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCggg                                                                           <  2:246418/134‑1 (MQ=255)
                                                gCGACCCCCCCCCCCTCTGCGCGCGGCGCGATTTCCGGCCCTTCGCTCAGCCGGCCCCCGTGCGCCCCCCGCCGGCGCCCGGCCGGCCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCGGGCGAT‑c                                                                     <  2:202316/135‑1 (MQ=255)
                                                  gATCACCCCACCCTCTTCGATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCCCGCCAGGCGCGGCTCTTCGATCTACGCGGTCAGGTCCAGCAGCACGCGGGCGAT‑Cgg                                                                   >  1:87954/1‑135 (MQ=255)
                                                     ccccccccccTCTTCGTGGGGCTGGATTTCCCGCACTGCCATCCGCCGCGCCAAGTGAGCCAGCAGCAGGCGCCCGGCCGGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCGGGCGAT‑CGGccc                                                                <  6:299313/133‑1 (MQ=255)
                                                     caccccccccTCTTCTATCGCCGCTATTCCAGCCACTGCTACCCCCCGGGCCACGGGCCCCCGCAGCCGGCGCCAGGCCGGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCGGGCGAT‑CGGccc                                                                <  2:106865/135‑1 (MQ=255)
                                                            cccTCTACGCTCGCCCCTATTTCCGCCACTCCGAACACCCGCCCCACGTCCCCCCCCCGCAGGCCCCCGGCGGGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCGGGCGAT‑CGGCCCGGACAgg                                                         <  2:159992/135‑1 (MQ=255)
                                                            cccTCCTCGAGCGGCTCGATTTCAGGCACTTCCCACAACCGCGCCAAGGGAGCCAGCCGCCTCCGCCCGGCCGGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCGGGCGAT‑CGGCCCGGACAgg                                                         <  1:36432/135‑1 (MQ=255)
                                                               tctTCGATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTTAGCAAGAAGCATGCGCCAGGCATGCCCCGGCTCTTCTATAACTCAGGTAACGTCCAGCAACAACCGGGCGAT‑CTGCACCGCCACGCCg                                                      >  4:97696/1‑135 (MQ=255)
                                                               gctTCGAGCGTCTAGATTGCCGGCAGTCGGCTCATCCGGCCCCTGTGCGCCAGCCGCAGGCGCCCGGCTGGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCGGGCGAT‑CGGCCCGGACAGGCCg                                                      <  6:312426/134‑1 (MQ=255)
                                                                     aTCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCAGGCCTGGCGCGGCTCTTCGATATAGGCGGTCAGGTCCAGCACCTCCCGGGCGATACGGCCC‑GACAGGCCGCAgcgc                                                >  4:161942/1‑135 (MQ=255)
                                                                         gCTCGATTCCCCCCACTCCCATCACCCGCCCCACGTGACCCACCACCAGCCCCCCGGCGGGCCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCGGGCGAT‑CGGCCCGGACAGGCCGATGCGCTGCa                                            <  1:325145/135‑1 (MQ=255)
                                                                                 tCAGCCACTCCGATCATCCGCCCAATCGGCGCCCGCCGCATGCGCCTGGCCGGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCGGGCGAT‑CGGCCCGGACAGGCCGATGCGCTGCAGgcggcg                                     <  5:25848/134‑1 (MQ=255)
                                                                                    ggCACTTCGACCCTCCGGCCCAAGTGCCCCAGCAGCTTCCGCCCGGCCTGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCGGGCGAT‑CGGCCCGGACAGGCCGATGCGCTGCAGGCGGCGCCaa                                 <  8:108619/135‑1 (MQ=255)
                                                                                                      gCCAAGTGAGCCAGCAGCATGCGCCAGGCCTGGCGCGGCGCTTAGATCTCGGCGGTCAGGTACAGCAGCTCGCGGGCGCT‑CGGCCCGGACAGGCCGCTGCGCGGCAGGCGGCGCCAAACGTTGGCCTGCTGCGGc               >  7:9571/1‑135 (MQ=255)
                                                                                                       ccAAGTGCCCCCGCCGCCGGCGCCCGGCGGGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCGGGCGAT‑CGGCCCGGACAGGCCGATGCGCTGCAGGCGGCGCCAAAGGTTGGCCTGCTGCGGCt              <  8:333376/135‑1 (MQ=255)
                                                                                                        cAAGTGAGCCAGCAGCATGCGCCAGGCCTGGCGCGGCTCTTCGATCTAGGCGGTCAGGTCCAGCAGCTCGCGGGCGAT‑CGGCCCGGACAGGCCGCTGCGCTGCAGGCGGCGCCAAAGGTTTGGCAGCTGCGGCtt             >  3:67980/1‑135 (MQ=255)
                                                                                                                   gccgcctGCGCCAGGCCTGGCGCGGCTCTTCGATCTCGGCGGGCAGGTCCAGCAGCTCGCGGGCGAT‑CGGCCCGGACAGGCCGATGCGCTGCAGGCGGCGCCAAAGGTTGGCCTGCTGCGGCTTGCTGCCCTGCa  <  5:232870/129‑1 (MQ=255)
                                                                                                                   gcagccTGCGCCCGGCCGGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCGGGCGAT‑CGGCCCGGACAGGCCGATGCGCTGCAGGCGGCGCCAAAGGTTGGCCTGCTGCGGCTTGCTGCCCTGCa  <  8:235522/135‑1 (MQ=255)
                                                                                                                               |                                                                                                                           
AGCTTGGCCAGGGTGGTGGTCTTGCCCATGCCCGCCGGGCCGACCATGGCGATCACCCCACCCTCTTCGATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCAGGCCTGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCGGGCGAT‑CGGCCCGGACAGGCCGATGCGCTGCAGGCGGCGCCAAAGGTTGGCCTGCTGCGGCTTGCTGCCCTGCA  >  ACB143/4927058‑4927307

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑