Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1434,927,1900TG46.4% ‑2.3 / 11.3 30Q192P (CAG→CCG) srp54Signal recognition particle 54 kDa protein
Reads supporting (aligned to +/- strand):  ref base T (10/5);  new base G (0/13);  total (12/18)
Fisher's exact test for biased strand distribution p-value = 2.51e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.15e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

AGCTTGGCCAGGGTGGTGGTCTTGCCCATGCCCGCCGGGCCGACCATGGCGATCACCCCACCCTCTTCGATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCAGGCCTGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCGGGCGAT‑CGGCCCGGACAGGCCGATGCGCTGCAGGCGGCGCCAAAGGTTGGCCTGCTGCGGCTTGCTGCCCTGCAGCTGATTCCAGGCCAGC  >  ACB143/4927058‑4927324
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aGCTTGGCCAGGGTGGTGGTCTTGCCCATGCCCGCCGGGCCGACCATGGCGATCACCCCACCCTCTTCGATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCAGGCCTgg                                                                                                                                       >  2:276986/1‑135 (MQ=255)
            gggggggtCTTCCCCATGCCCGCCGGGCCGACCATGGCGATCACCCCACCCTCTTCGAGCGGCTCTATTTCAGGCACTTCCATCATCCGTCCCAAGTGAGCCAGCAGCATGCGCCAGCCCTGGCGCGGCTCTTc                                                                                                                            <  6:74670/129‑1 (MQ=255)
              ggtggtCTTGACCATGCACGCCGGGCCGACCATGGCGATCACCCCCCCCTCTTCGATCGGCTCGATTTCAGGCACTTCGATCATACGTGACAAGTGAGCCAGCAGCATGCCCCCAGCCTGGCGCCGccctgggct                                                                                                                         >  6:48057/1‑130 (MQ=255)
                        cccATGCCCGCGGGGCCGACCTGGGCTATCACCCCACCCTGTTCGATCGGCTCTATTTCAGGCACTTCGAACATCCTTGCCAAGTGAGCCTGCAGAATGCCCCAGGCGTGGCGCGGCTCTTCGATCTcggcg                                                                                                                  <  6:58074/132‑1 (MQ=255)
                        cccATGCCCGCCGGGCCGACCATGGCGATCACCCCACCCTCTTCGATCGGCTCGATTTCAGGCACTTAGATCATCCGTGCAAAGTGAGCCAGCAGCATGCGCCAGGGCTGGAGCGGCTCGTAGATCTgggagg                                                                                                                 >  4:271928/1‑133 (MQ=255)
                        cccAGGCCCGCCGGGCCGACCTTGGCGACCCCCCCCCCCTCTTCTATCGGCGCGATTTCAGCCACTTCGATCAGCCGTCCCAAGGGACCCAGCAGCAGGCGCCTGGCCGGGCGCGGCTCTTCGATCTcggcg                                                                                                                  <  6:259368/132‑1 (MQ=255)
                               ccgccgGGCCGACCATGGCGATCACCCCACCCTCTTCGATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCAGGCCTGGCGCGGGACGTCGCTCTCGGCGGTGAggggca                                                                                                        >  3:200725/1‑131 (MQ=255)
                                cgccgGGCCGACCATGGCGATCACCCCACCCTCTTCGATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCCCGCCAGGGGCGGCTCGTCGGTGTGGGCGGGCGGGTGcag                                                                                                       >  5:18982/1‑135 (MQ=255)
                                cgccgGGCCGACCATGGCGATCACCCCACCCTCTTCGATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCAGGCCTGGCGCGGCGCGTCGATCTCGGCGGTCAGGTCca                                                                                                        >  1:246418/1‑134 (MQ=255)
                                            cATGGCGACCCCCCCCCCCTCTGCGATCGGCTCTATTCCAGCCACTTCTATCATCCGTCCCAAGTGCCCCCGCACCAGGCGCCCGGCCGGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCggg                                                                                            <  2:246418/134‑1 (MQ=255)
                                                gCGACCCCCCCCCCCTCTGCGCGCGGCGCGATTTCCGGCCCTTCGCTCAGCCGGCCCCCGTGCGCCCCCCGCCGGCGCCCGGCCGGCCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCGGGCGAT‑c                                                                                      <  2:202316/135‑1 (MQ=255)
                                                  gATCACCCCACCCTCTTCGATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCCCGCCAGGCGCGGCTCTTCGATCTACGCGGTCAGGTCCAGCAGCACGCGGGCGAT‑Cgg                                                                                    >  1:87954/1‑135 (MQ=255)
                                                     ccccccccccTCTTCGTGGGGCTGGATTTCCCGCACTGCCATCCGCCGCGCCAAGTGAGCCAGCAGCAGGCGCCCGGCCGGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCGGGCGAT‑CGGccc                                                                                 <  6:299313/133‑1 (MQ=255)
                                                     caccccccccTCTTCTATCGCCGCTATTCCAGCCACTGCTACCCCCCGGGCCACGGGCCCCCGCAGCCGGCGCCAGGCCGGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCGGGCGAT‑CGGccc                                                                                 <  2:106865/135‑1 (MQ=255)
                                                            cccTCTACGCTCGCCCCTATTTCCGCCACTCCGAACACCCGCCCCACGTCCCCCCCCCGCAGGCCCCCGGCGGGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCGGGCGAT‑CGGCCCGGACAgg                                                                          <  2:159992/135‑1 (MQ=255)
                                                            cccTCCTCGAGCGGCTCGATTTCAGGCACTTCCCACAACCGCGCCAAGGGAGCCAGCCGCCTCCGCCCGGCCGGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCGGGCGAT‑CGGCCCGGACAgg                                                                          <  1:36432/135‑1 (MQ=255)
                                                               tctTCGATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTTAGCAAGAAGCATGCGCCAGGCATGCCCCGGCTCTTCTATAACTCAGGTAACGTCCAGCAACAACCGGGCGAT‑CTGCACCGCCACGCCg                                                                       >  4:97696/1‑135 (MQ=255)
                                                               gctTCGAGCGTCTAGATTGCCGGCAGTCGGCTCATCCGGCCCCTGTGCGCCAGCCGCAGGCGCCCGGCTGGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCGGGCGAT‑CGGCCCGGACAGGCCg                                                                       <  6:312426/134‑1 (MQ=255)
                                                                     aTCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCAGGCCTGGCGCGGCTCTTCGATATAGGCGGTCAGGTCCAGCACCTCCCGGGCGATACGGCCC‑GACAGGCCGCAgcgc                                                                 >  4:161942/1‑135 (MQ=255)
                                                                         gCTCGATTCCCCCCACTCCCATCACCCGCCCCACGTGACCCACCACCAGCCCCCCGGCGGGCCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCGGGCGAT‑CGGCCCGGACAGGCCGATGCGCTGCa                                                             <  1:325145/135‑1 (MQ=255)
                                                                                 tCAGCCACTCCGATCATCCGCCCAATCGGCGCCCGCCGCATGCGCCTGGCCGGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCGGGCGAT‑CGGCCCGGACAGGCCGATGCGCTGCAGgcggcg                                                      <  5:25848/134‑1 (MQ=255)
                                                                                    ggCACTTCGACCCTCCGGCCCAAGTGCCCCAGCAGCTTCCGCCCGGCCTGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCGGGCGAT‑CGGCCCGGACAGGCCGATGCGCTGCAGGCGGCGCCaa                                                  <  8:108619/135‑1 (MQ=255)
                                                                                                      gCCAAGTGAGCCAGCAGCATGCGCCAGGCCTGGCGCGGCGCTTAGATCTCGGCGGTCAGGTACAGCAGCTCGCGGGCGCT‑CGGCCCGGACAGGCCGCTGCGCGGCAGGCGGCGCCAAACGTTGGCCTGCTGCGGc                                >  7:9571/1‑135 (MQ=255)
                                                                                                       ccAAGTGCCCCCGCCGCCGGCGCCCGGCGGGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCGGGCGAT‑CGGCCCGGACAGGCCGATGCGCTGCAGGCGGCGCCAAAGGTTGGCCTGCTGCGGCt                               <  8:333376/135‑1 (MQ=255)
                                                                                                        cAAGTGAGCCAGCAGCATGCGCCAGGCCTGGCGCGGCTCTTCGATCTAGGCGGTCAGGTCCAGCAGCTCGCGGGCGAT‑CGGCCCGGACAGGCCGCTGCGCTGCAGGCGGCGCCAAAGGTTTGGCAGCTGCGGCtt                              >  3:67980/1‑135 (MQ=255)
                                                                                                                   gccgcctGCGCCAGGCCTGGCGCGGCTCTTCGATCTCGGCGGGCAGGTCCAGCAGCTCGCGGGCGAT‑CGGCCCGGACAGGCCGATGCGCTGCAGGCGGCGCCAAAGGTTGGCCTGCTGCGGCTTGCTGCCCTGCa                   <  5:232870/129‑1 (MQ=255)
                                                                                                                   gcagccTGCGCCCGGCCGGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCGGGCGAT‑CGGCCCGGACAGGCCGATGCGCTGCAGGCGGCGCCAAAGGTTGGCCTGCTGCGGCTTGCTGCCCTGCa                   <  8:235522/135‑1 (MQ=255)
                                                                                                                               tggCCGGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCGGGCGAT‑CGGCCCGGACAGGCCGATGCGCTGCAGGCGGCGCCAAAGGTTGGCCTGCTGCGGCTTGCTGCCCTGCAGCTGATTCCAgg       <  8:178236/134‑1 (MQ=255)
                                                                                                                               cggCCTGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCGGGCGAT‑CGGCCCGGACAGGCCGATGCGCTGCAGGCGGCGCCAAAGGTTGGCCTGCTGCGGCTTGCTGCCCTGCAGCTGATTCCAgg       <  8:217907/134‑1 (MQ=255)
                                                                                                                                 gCCTGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCGGGCGAT‑CGGCCCGGACAGGCCGATGCGCTGCAGGCGGCGCCAAAGGTTGGCCTGCGGCGGCTTGCTGCCCTGCAGCTGATTCCAgg       >  3:240484/1‑133 (MQ=255)
                                                                                                                                    tGGCTCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCGGGCGAT‑CGGCCCGGACAGTCCGATGCGCTGCAGGCGGCTACAAAGGTTGGCCTGCTGCGGCTTGCTGCCCTGCAGCTGATTCCAGGCCAGc  <  6:262119/135‑1 (MQ=255)
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AGCTTGGCCAGGGTGGTGGTCTTGCCCATGCCCGCCGGGCCGACCATGGCGATCACCCCACCCTCTTCGATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCAGGCCTGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCGGGCGAT‑CGGCCCGGACAGGCCGATGCGCTGCAGGCGGCGCCAAAGGTTGGCCTGCTGCGGCTTGCTGCCCTGCAGCTGATTCCAGGCCAGC  >  ACB143/4927058‑4927324

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑