Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1435,693,1750AC54.5% ‑0.8 / 25.0 55S236R (AGC→CGC) pyrC'Dihydroorotase‑like protein
Reads supporting (aligned to +/- strand):  ref base A (10/15);  new base C (9/21);  total (19/36)
Fisher's exact test for biased strand distribution p-value = 5.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.97e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GGCCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCAGC  >  ACB143/5693042‑5693307
                                                                                                                                     |                                                                                                                                    
ggCCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGGGTGCGCACTTCCGCCAGATCACCAg                                                                                                                                     >  6:176709/1‑135 (MQ=255)
     aCGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGGCCTTCAGCCAGATCACCCGCGccc                                                                                                                                >  8:348217/1‑135 (MQ=255)
     aCGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCCCGTGAGCCAGATCACCCCCGccc                                                                                                                                >  1:18285/1‑135 (MQ=255)
     aCGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGACGTGCGTGCGCACTTCAGCCAGCTCACCAGCGccc                                                                                                                                >  4:305168/1‑135 (MQ=255)
        acggTGCCATGGCCAGCTGCCTCGGCCTGCCGGGAATCCCGGAGAGCGCCGAAACGGTGCCCATGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGAACACCCGCGCCCGTg                                                                                                                             <  2:44005/133‑1 (MQ=255)
        aaGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCGGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGGGCGCACTTCAGCCAGATCACCCGCGCCCGTg                                                                                                                             <  6:249485/135‑1 (MQ=255)
         aGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGGGGGTGGGGCCGTCACCCCGAACACCACCGccccggg                                                                                                                            >  2:319962/1‑131 (MQ=255)
            tGCCATGGCCAGCTGCCTCGGCCTGCCGGGCAGCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGGGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGgcg                                                                                                                          <  7:343666/134‑1 (MQ=255)
              ccATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTTCGTGCGCGCTTAAGCCAGATCACCCCCGCCCCTGgcgcgc                                                                                                                       >  5:138927/1‑135 (MQ=255)
              ccATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGGTCGCCCGTAAGCCCGATCACCCGCGCCCCTGgcgcgc                                                                                                                       >  1:30263/1‑135 (MQ=255)
               cATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGcgcgcg                                                                                                                      >  2:256885/1‑135 (MQ=255)
                 tGGCCAGCTGCCTGGGCCGGCCGGGCATCCCGGCGCGCGCCGAAACCGGGGCCCTGGCGGGCAACCTGCTGCTGGGGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGACCCCCCGCGCCCGGGGCGCGCGGc                                                                                                                    <  6:101514/135‑1 (MQ=255)
                 gggCCAGCTGCCGCGGCCGGCCGGGCATCCCGGCGCGCGCCGAACCGGGGGCCCTGGCGCGCAACCTGCGGCGGGTGCAACAGCGCGGCGGGCGTGCGCACTCCCCCCAGACCCCCCGCGCCCGGGGCGCGCGGc                                                                                                                    <  2:9902/134‑1 (MQ=255)
                  ggCCAGCGTCAGCGGCCTGCCGGGCATCCCGGCGCGCGCCGAACCCGGGGCCCTGGCGCGCAACCTGCGCGGGGGGGAACATTGGGGCGTGCGTGCTCACTTCACCCAGCTCACCAGCGCCCGTGGCGCGCGGCt                                                                                                                   <  7:18356/135‑1 (MQ=255)
                      aGCTGCCGCGGCCGGCCGGCCACCCCGGAGAGCGCCTAAACCGGGGCCCTGGCGCGCAACCTGCTGCTGGGGCAACAGCGCGGGGGGCGTGCGCACTTCCCCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATc                                                                                                               <  6:303534/135‑1 (MQ=255)
                           ccTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGACCAGAGCGGCGTGCGTGCGCCCTTCCGCCAGATCACCCGCGCCCGTGGCGCGCGGCTGATCgcccc                                                                                                          >  1:275344/1‑134 (MQ=255)
                            cgcGGCCTGCGGGGCACCCCGGAGCGCGCCTAACCCGGGGCCCGGGCGCGCAACCTGGTGCTGGTGGAACAGAGCGGGGTGCGTGCGCACTTCACCCACATCACCCGCGCCCGTGGCGCGCGGCTGATCgcccag                                                                                                         <  6:104143/133‑1 (MQ=255)
                            cTCGGCCTGCCGGGCAGCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGCAACAGAGGGGGGGGCGGGCGCACTTCAGCCACAACCCCAGCGCCCGGGGCGCGCGGCTGATCgcccag                                                                                                         <  2:130773/135‑1 (MQ=255)
                            cTCGGCCGGCCGGGCAGCCCGGAGCGCGCCGAAACGGGGCCCCGGGCGCGCACCGTGGGGCGGGGGCAACAGCGGGGGGGGGGGGCGCACTACCCCCACACCCCCCGCGCCCGGGGCGCGCGGCTGATCgcccag                                                                                                         <  4:182526/135‑1 (MQ=255)
                             tCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGGGCGCACTTCCCCCAGATCACCCGCGCCCGTGGCGCGCGGCTGATCgcccagg                                                                                                        <  7:52169/135‑1 (MQ=255)
                             tCGGCCGGCCGGGCATCCCGGAGAGCGCCGAAACGGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGGGTGCGCACTACAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCgcccagg                                                                                                        <  1:204224/135‑1 (MQ=255)
                                   tGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGGGGGCCCGTCAGCCCGATCCCCAGCGCCCGGGGCGCGCGGCGGATCGcccccgccccgg                                                                                                  >  6:292627/1‑135 (MQ=255)
                                   tGCCGGGCACCCCGGAGCGCGCCGAAACCGGGGCCCGGGCGCGCACCCTGCTGCTGGTGGACCAGAGCGGCGGGCGTGCGCACTCCACCCAGACCCCCCGCGCCCGGGGCGCGCGGCTGATCGcccaggcccagg                                                                                                  <  2:215472/135‑1 (MQ=255)
                                                ggAGAGCGCCGAAACCGGGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGGGCGCACTTCAGCCAGATCACCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGcc                                                                                     <  2:207852/135‑1 (MQ=255)
                                                  agagCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGGGCCCGTCCGCCCGATCACCCGCGCCCGGGGCGCGCGGCGGGTCGCCCCGGCCCCGGCCGTGGGCCGGGCgg                                                                                   >  5:168622/1‑135 (MQ=255)
                                                      cgcCGAACCGGTGGCCCTGGCGCGCAACCTGCGGTTGGGGGAACAGCGCGGGGGGCGTGCGCAATTCAGCCAGATCACCCGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAc                                                                               <  2:18383/135‑1 (MQ=255)
                                                      cgcCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCGGGGGGAACAGCGCGGCGGGCGTGCGCACTTCAGCCAGAACACCAGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAc                                                                               <  6:307345/135‑1 (MQ=255)
                                                      cgcCGAAACCGTGGCCCGGGCGCGCACCCTGCGGCGGGTGGACCAGCGCGGCGTGCGTGCGCACTTCAGCCAGATCCCCAGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAc                                                                               <  5:164629/135‑1 (MQ=255)
                                                         cGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCCGCTCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCCCGCCCACGCCTTGGGCCTGCCGGTTAccgc                                                                            >  8:31572/1‑135 (MQ=255)
                                                         cGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCCGCGCCCGTGGCGCGCGGCTGATCGCCCCGGCCCCGGCCGTGGGCCTGCCGGTAAccgc                                                                            >  6:355806/1‑135 (MQ=255)
                                                           aacccGGGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGCGCGGCGGGCGGGCGCACTTCAGCCAGATCACCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAccgccg                                                                          <  1:272143/132‑1 (MQ=255)
                                                           aaaCCGTGGCCCTGGCGCGCAACCTGCTGCGGGTGGAACCGAGCGGCGTGCGTGCGAACTTCAGCCAGATCACCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAccgccg                                                                          <  1:30427/135‑1 (MQ=255)
                                                           aaaCCGGGGCCCTGGCGCGCAACCTGCGGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCATATCCCCCGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAccgccg                                                                          <  5:133754/135‑1 (MQ=255)
                                                                gTGGCCCTGGCGCGCAACGTGCTGCTGGTGGAACAGCGCGGCGGGCGTGCGCACTTCCGCCAGATCCCCAGCGCCGGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                                     <  8:104240/135‑1 (MQ=255)
                                                                    cccTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGGGTGCGCCCTTGCGCCAGCTCACCCGCGCCCGGGGCGCGCGGCGGATCGCCCAGGCCCACGCCGTGGGCCGGCCGGGAACCGCCGATGTAgcgc                                                                 >  6:71092/1‑135 (MQ=255)
                                                                    cccTGGCGCGCAACCTGCTGCTGGTGCAACAGAGCGGGGGGCGGGCGCACTTCAGCCAGATCACCAGCGCCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAgcgc                                                                 <  2:18285/135‑1 (MQ=255)
                                                                    cccGGGCGCGAACCGTGCTGCGGGTGGAACTGCGGGGCGGGGGTGCGCACTTCCGCCAGAACCCCCGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAgcgc                                                                 <  7:317679/135‑1 (MQ=255)
                                                                            cgcAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCCGCTAACCCTCGCCCCTGGCGCGCGGCGGCTCGCCCAGGCACCGCCCTTGGGCCTGCCGGGTACCGCCCCAGTATCGCTGTGcccg                                                         >  8:326704/1‑133 (MQ=255)
                                                                                        gtggtgcACCAGGGGGGGGGGCGGGCGCACTTCAGCCAGATCACCCGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACg                                             <  4:180331/134‑1 (MQ=255)
                                                                                              gAACAGAGCGGCGTGCGGGCGCACTTCACCCAGCTCACCACCGCCCGTGGCGCGCGGCTGATCTCCCCCGCCCCGGCCTTGGGCCTGGCGGTTACCCCCGCTGTATCGCTGTAGCAGCTGATAATCACGGCCGAg                                       >  2:301389/1‑135 (MQ=255)
                                                                                               aaCAGCGCGGCGGGCGTGCGCACTTCCGCCAGAGCCCCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGt                                      <  2:250702/135‑1 (MQ=255)
                                                                                                  agagCGGCGTGCGGGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGc                                   <  3:102699/135‑1 (MQ=255)
                                                                                                      cggcgTGCGTGCGCACTTCAGCCAGACCCCCAGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTgcg                               <  2:71070/135‑1 (MQ=255)
                                                                                                       ggcgTGCGGGCGCACTTCAGCCACATCCCCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTgcgc                              <  1:41890/135‑1 (MQ=255)
                                                                                                           ggcgtgcgCACTGCAGCCAGAACACCAGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt                          <  4:232266/134‑1 (MQ=255)
                                                                                                           ggcgggcgCACTTCAGCCAGACCACCCGCGCCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt                          <  2:275344/134‑1 (MQ=255)
                                                                                                            gcgtgcgCACTTCCGCCAGACCACCCGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGtt                         <  5:314264/135‑1 (MQ=255)
                                                                                                             ggtgcgCACTTCCGCCAGAGCACCCCCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTtc                        <  1:194885/134‑1 (MQ=255)
                                                                                                                gcgcACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTcc                     <  1:256885/135‑1 (MQ=255)
                                                                                                                  gcACTTCAGCCAGATCACCCGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAg                   <  8:171383/135‑1 (MQ=255)
                                                                                                                         aGCCTGATCACCCGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTa             <  6:138927/134‑1 (MQ=255)
                                                                                                                                   ccAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGCGTTCTCCAGCCTGTACCCCGTGCAgc  >  7:327595/1‑135 (MQ=255)
                                                                                                                                   ccAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCAgc  >  3:183674/1‑135 (MQ=255)
                                                                                                                                   ccAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTGCTCCAGCCTGTACCACGTGCAgc  >  8:343375/1‑135 (MQ=255)
                                                                                                                                   ccAGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCAgc  <  2:11637/135‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                    
GGCCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCAGC  >  ACB143/5693042‑5693307

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑