| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB143 | 6,043,196 | 0 | C | A | 36.8% | 91.2 / 35.8 | 87 | A37S (GCC→TCC) | puuD_3 | Gamma‑glutamyl‑gamma‑aminobutyrate hydrolasePuuD |
| Reads supporting (aligned to +/- strand): ref base C (29/26); new base A (0/32); total (29/58) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.35e-08 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCGCCATTGTAATGATGCGGCTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGG‑C‑AAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCGTGTGCAGGTAC > ACB143/6043069‑6043327 | gcgcCATTGTAATGATGCGGCTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGG‑C‑AAGATCAGTGGCAAGCCGGCAAA‑gccg > 2:197559/1‑135 (MQ=255)gcgcCATTGTAATGATGCGGATCTATATTTGAAGGCGAGCCGGTAAAAACAATGACGTGCAGGCGAGCCAGCAGCGCTTGAGTGTCGCTGCCGCCATCACGGGCCGG‑C‑AAGATCAGTGGCAAGCCGGCAAA‑gccg > 1:348472/1‑135 (MQ=255) ccATTGTAATGATGCGGCTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGG‑C‑AAGATCAGTGGCAAGCCGGCAAA‑gccggcc > 5:238698/1‑135 (MQ=255) gTAATGATGCGGCTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGG‑C‑AAGATCAGTGGCAAGCCGGCAAA‑GCCGgccgccgg > 5:232050/1‑133 (MQ=255) tgatgCGGCTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGG‑C‑AAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGAc > 8:343023/1‑134 (MQ=255) atgCGGCTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGG‑C‑AAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATa > 2:77743/1‑135 (MQ=255) tgCGGCTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGG‑C‑AAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATAc > 6:339182/1‑135 (MQ=255) cGGCTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGG‑C‑AAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACtt > 3:8980/1‑135 (MQ=255) cTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGG‑C‑AAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTc > 1:142144/1‑135 (MQ=255) cTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGG‑C‑AAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTc > 8:254539/1‑135 (MQ=255) cGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGG‑C‑AAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGc > 2:17563/1‑135 (MQ=255) cGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGG‑C‑AAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGc > 1:319376/1‑135 (MQ=255) cGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGG‑C‑AAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGc > 6:114073/1‑135 (MQ=255) tatatTTCAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGGCGCTGCCGCCATCACGGGCCGG‑C‑AAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGcc < 6:81627/134‑1 (MQ=255) cggCGCGCCGGTAAAAACACTGCCGGGCAGGCGAGCCAGCCGCGCTTGCGTGTCGCGGCCGCCACCACGGGCCGG‑C‑AAGATCAGTGCCAAGCCGGAAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCgtgt < 7:154172/133‑1 (MQ=255) acccGGTAAAAACAATGCCGTCCCGGCGAGCCAGCACGGCTTGCGTGGCGCTGCCGCCATCCCGGGCCGG‑C‑AAGATCAGGGCAAAGCCGGAAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCgagg < 2:328647/133‑1 (MQ=255) gCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGG‑C‑AAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCgagga > 5:71019/1‑135 (MQ=255) gCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGG‑C‑AAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCgagga > 2:77906/1‑135 (MQ=255) gCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGG‑C‑AAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCgagga > 2:265580/1‑135 (MQ=255) gCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGG‑C‑AAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCgagga > 1:116337/1‑135 (MQ=255) gCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGG‑C‑AAGATCAGTGGCAAGCCGGAAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACctg < 5:250644/135‑1 (MQ=255) ccggCGTGCAAGCTGCGCTTGGGTGTCGCTGCCGCAACCCGGGGCCGG‑C‑AACATCTGTGGCACGCCGCAAAA‑CCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACg < 2:316874/133‑1 (MQ=255) cAGGCGAGCCAGCAGCGCTGGGGTGGCGCGGCCGCCAGCCCGGGCGGC‑A‑AATATCCGGGGCACGCGGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACg < 1:310418/135‑1 (MQ=255) aGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGG‑C‑AAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGCCATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACg > 1:87460/1‑134 (MQ=255) aGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGG‑C‑AAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACg > 7:84071/1‑134 (MQ=255) aGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGG‑C‑AAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACg > 4:261358/1‑134 (MQ=255) aGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGG‑C‑AAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACg > 1:82147/1‑134 (MQ=255) aGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGG‑C‑AAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACg < 5:339182/134‑1 (MQ=255) cagcagCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGG‑C‑AAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGGGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCt > 6:177488/1‑135 (MQ=255) gccgcgtTTGCGGGTCGAGGCCGCCAGACCGGGCCGC‑A‑ATGATCTGTGGCAAGCCGGAAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGa < 6:194669/132‑1 (MQ=255) gcagcGTTGGGGTGTCGCGGCCGCCTACAGGGGCCGG‑C‑ATGATCGGGGGCACGCGGCAAAA‑GCGGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGa < 3:343149/135‑1 (MQ=255) gcagcGCTTGCGTGTCGCTGCCGCAATCACGGGCCGG‑A‑AAGATCTGTGGCAAGCCGTAAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGa < 6:324242/135‑1 (MQ=255) gCGTGTCGCTGCCGCCATCACGGGCCGG‑C‑AAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATc > 5:324242/1‑134 (MQ=255) gCGTGTCGCTGCCGCCATCACGGGCCGG‑C‑AAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATc > 7:220396/1‑134 (MQ=255) gtgtCGCTGCCGCCATCACGGGCCGG‑C‑AAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCaaa > 2:38938/1‑135 (MQ=255) gtCGCTGCCGCAATC‑CGGGTCGGTC‑TAGATAATTGGCAAGCCGTAAAA‑GCGGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAgg < 1:100048/135‑1 (MQ=255) cTGCCGCCATCACGGGCCGG‑C‑AAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGCCGGCAGGCGCTGACCCCGATCAAAGGGAcc > 2:140546/1‑135 (MQ=255) ggccgccAGCACGGGCCGG‑C‑AAGATCTGGGGCACGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCg < 8:291182/134‑1 (MQ=255) cgccATCACGGGCCGG‑C‑AAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCAt < 8:27845/135‑1 (MQ=255) ccATCACGGGCCGG‑C‑AAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCAttt < 1:77743/135‑1 (MQ=255) aTCACGGGCCGG‑C‑AAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgc > 3:164526/1‑135 (MQ=255) ccggggcggg‑c‑ACCACCAGTGCCATCCCGCCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 2:115472/132‑1 (MQ=255) cccGGGCCGG‑C‑AATATCTGTGGCACGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 4:203788/133‑1 (MQ=255) cccGGGCCGG‑C‑AACATCAGTGCCACGCCGGAAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 1:39524/133‑1 (MQ=255) caggggcggg‑c‑ATGAGCTGGGGAACGCGGGAAAA‑GCGGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 8:309397/132‑1 (MQ=255) caggggcggg‑c‑AAGAGCAGTGGCAAGCCGCAAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 2:320657/132‑1 (MQ=255) caggggCGGG‑A‑ATGATCTTTGCCAAGCCGGAAAA‑GCGGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 2:58808/132‑1 (MQ=255) caggggCCGG‑C‑AAGATCTGTGGCAAGCCGGAAAA‑GCGGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 4:55032/132‑1 (MQ=255) caggggCCGC‑A‑AACATCCGGGCCACGCCGGAAAA‑CCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 2:177287/132‑1 (MQ=255) cACGGGCGGG‑C‑ACTAGCAGTGGCACGCCGGCAAA‑GCGGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 2:347473/135‑1 (MQ=255) cACGGGCCGG‑C‑AAGATCCGGGGCACCCCGGAAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 7:144592/135‑1 (MQ=255) cACGGGCCGG‑C‑AAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 8:277753/135‑1 (MQ=255) cACGGGCCGG‑C‑AAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 8:190475/135‑1 (MQ=255) cACGGGCCGG‑C‑AAGATCAGTGGCAAGCCGGAAAA‑GCGGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 7:40452/135‑1 (MQ=255) cACGGGCCGG‑C‑AAGATCAGTGGCAAGCCGGAAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 1:77906/135‑1 (MQ=255) cACGGGCCGG‑C‑AAGATCAGTGGCAAGCCGGAAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 6:238698/135‑1 (MQ=255) cACGGGCCGG‑C‑AAGATCAGTGGCAAGCCGGAAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 1:197559/135‑1 (MQ=255) cACGGGCCGG‑C‑AAGATCAGTGGCAAGCCGCCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 8:278638/135‑1 (MQ=255) cACGGGCCGG‑C‑AAGATCAGTGCCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 1:286995/135‑1 (MQ=255) cACGGGCCGG‑C‑AAGATCAGGGGCACGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 6:82620/135‑1 (MQ=255) cACGGGCCGG‑C‑AACATCAGTTGCATGCGGGAAAA‑GCGGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTTCTGACGGCAGGCGCTGACACCTATCAAATGGACCGCATTTgcgc < 7:269215/135‑1 (MQ=255) cACGGGCCGG‑A‑ATGATCAGTGGCAAGCCGTCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 1:283749/135‑1 (MQ=255) cACGGGCCGG‑A‑AATATCTGTGGCAAGCCGGAAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 5:59930/135‑1 (MQ=255) cACGGGCCGG‑A‑AATATCCGTGGCAAGCCGGAAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 4:199086/135‑1 (MQ=255) cACGGGCCGG‑A‑AATATCAGTGGCAAGCCGGAAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 2:70502/135‑1 (MQ=255) cACGGGCCGG‑A‑AATATCAGTGGCAAGCCGGAAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 4:59632/135‑1 (MQ=255) cACGGGCCGG‑A‑AAGATCGGGGCCAGGCCGGAAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 4:304372/135‑1 (MQ=255) accGGGCCGG‑C‑AATATCAGTGGCAAGCCGGAAAA‑GCGGGCCGCCTCGACATACTTGGCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 8:244680/133‑1 (MQ=255) ccGGGGCGG‑CAAAGATCAGT‑GCAACGCGGCAAACGCCGCCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 6:33479/134‑1 (MQ=255) cggg‑C‑AAGATCCGTGGCACGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATgg < 3:297380/133‑1 (MQ=255) cGG‑C‑AAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATggg < 2:281284/135‑1 (MQ=255) g‑C‑AAGATCAGTGGCAAGCCGGCAAA‑GCGGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCt < 2:116337/135‑1 (MQ=255) aaataTCAGTGGAACGCCGGAAAC‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCtt < 2:326310/131‑1 (MQ=255) gATCAGTGGCAAGCCGGAAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTaca < 7:32189/135‑1 (MQ=255) aGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCgt > 5:306936/1‑135 (MQ=255) gTGGCAAGCCGGAAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCgt < 1:2615/134‑1 (MQ=255) tGGCACGCCGGAAAC‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCgtgt < 5:292739/135‑1 (MQ=255) tGGCAAGCGGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCgtgt < 1:78015/135‑1 (MQ=255) tGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCgtgt < 7:118094/135‑1 (MQ=255) ggCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCgtgt < 3:156382/134‑1 (MQ=255) ggCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGATTACACCgtgt < 1:286705/134‑1 (MQ=255) cacgCCGGAAAA‑GCGGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCGTGTGCa < 8:216829/132‑1 (MQ=255) aGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCGTGTGCAgg < 8:105659/135‑1 (MQ=255) cGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGTGACCGCATTTGCGCTCATGGGCTTACACCGTGTGCAGGtta < 8:84071/135‑3 (MQ=255) cGGAAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCGTGTGCAGGTAc < 5:137053/135‑1 (MQ=255) ggCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 5:55052/107‑1 (MQ=255) ggCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc > 6:55052/1‑107 (MQ=255) | GCGCCATTGTAATGATGCGGCTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGG‑C‑AAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCGTGTGCAGGTAC > ACB143/6043069‑6043327 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |