Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	ACB143	5,386,514	T→C	intergenic (‑523/+1128)	CJ019_05070 ← / ← nptII	hypothetical protein/neomycin phosphotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB143	5,386,514	0	T	C	100.0%	73.9 / NA	20	intergenic (‑523/+1128)	CJ019_05070/nptII	hypothetical protein/neomycin phosphotransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (7/13);  total (7/13)

TTTTTTATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCCGT‑‑CGT‑CA‑GGATC‑‑A‑‑TCCGGGCGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTGGG  >  ACB143/5386412‑5386605                                                                                                               |                                                                                            ttttttATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTGTACGGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGcc                                                                 >  1:7438/1‑135 (MQ=255)            tAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTGTACGGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGAt                                                       <  5:337162/134‑1 (MQ=255)                   aaTTCATCGAATTCTATCATTTAAGACCCAAAAATGACAACTCGGCCGGCTTGTCGACGACGGCGGTCTCAGTGGAGT‑AC‑GGTAC‑‑AACCCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTcc                                               <  7:343788/135‑1 (MQ=255)                     ttGATCGAATTCTTTCATTTAACACCCTAATATGCCATCTCGGCCGGCTTGTCGACTACGGCGGTCTCAGTGGGGT‑AC‑GGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCgg                                             <  5:218344/135‑1 (MQ=255)                     ttGAGCGAATGCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTGTACGGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCgg                                             <  6:244554/135‑1 (MQ=255)                                 tttCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTGTACGGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTa                                  <  2:206841/134‑1 (MQ=255)                                  ttCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTGTACGGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTAtt                                <  6:334475/135‑1 (MQ=255)                                      tttAAGCCCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTGTACGGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTgcgc                            <  3:303300/135‑1 (MQ=255)                                      tttAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTGTACGGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTgcgc                            <  2:310793/135‑1 (MQ=255)                                      tttAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTGTACGGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTgcgc                            <  5:4928/135‑1 (MQ=255)                                                   tatGCCATCTCGGCCGGCTTGTGGACGACGGCGGTCTCAGT‑‑GGTGTACGGTACAAA‑‑CCCAGTCGCTAGCAACGCATGAGAAAGCGCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCTGGTGCCAgg               <  8:282573/135‑1 (MQ=255)                                                        cATCTCGGCCGGCTTGGCGACGACGGCGGTCTCAGT‑‑GGTGTACGGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTg          <  2:30073/135‑1 (MQ=255)                                                           ctcGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTGTACGGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTTTTGCGCTGCGGGGGCCAGGGCGTGccc       >  5:232818/1‑135 (MQ=255)                                                             cggccggcTTGTCGACGACGGCGGTCTCAGT‑‑GGTGTACGGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCtt     >  5:119186/1‑135 (MQ=255)                                                              ggccggcTTGTCGACGACGGCGGTCTCAGT‑‑GGTGTACGGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTg    >  3:104070/1‑135 (MQ=255)                                                               gccggcTTGTCGACGACGGCGGTCTCAGT‑‑GGTGTACGGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCtt     <  2:18121/133‑1 (MQ=255)                                                               gccggcTTGTCGACGACGGCGGTCTCAGT‑‑GGTGTACGGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCtt     <  1:176249/133‑1 (MQ=255)                                                               gccggcTTGTCGACGACGGCGGTCTCAGT‑‑GGTGTACGGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTgg   >  7:104309/1‑135 (MQ=255)                                                                ccggcTTGTCGACGACGGCGGTCTCAGT‑‑GGTGTACGGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTACGGGGGCTTTTTTATTGCGCTGCGGGTGCCCGGGCGTGCCCTTggg  >  3:328745/1‑135 (MQ=255)                                                                ccggcTTGTCGACGACGGCGGTCTCAGT‑‑GGTGTACGGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATAACCTTACGGGGGCTTTTTTATTTCGGTTCGGGTGCCAGGGCGTGCCCGTggg  >  3:188200/1‑135 (MQ=255)                                                                                                               |                                                                                            TTTTTTATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCCGT‑‑CGT‑CA‑GGATC‑‑A‑‑TCCGGGCGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTGGG  >  ACB143/5386412‑5386605

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑