Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	ACB143	5,390,241	G→A	G112D (GGC→GAC)	ligH →	10‑formyltetrahydrofolate synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB143	5,390,241	0	G	A	100.0%	102.3 / NA	33	G112D (GGC→GAC)	ligH	10‑formyltetrahydrofolate synthetase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (12/21);  total (12/21)

CGGCCGGCGAAGGCAAGACCACGACCACCGTGGGCCTGGCCGACGGCCTCAACCGCATCGGCAAGAAGACCGTGGCGGCGCTGCGCGAGCCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAGGTCGTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGACTTCCATGCCATCACCTCGGCGAACAACCTGCTCGCCGCGCTGATCGACAACCACATCTACTGGGGCAACAA  >  ACB143/5390109‑5390370                                                                                                                                     |                                                                                                                                  cggccggcGAAGGCAAGACCACGACCACCGTGGGCCTGGCCGACGGCCTCAACCGCATCGGCAAGAAGACCGTGGCGGCGCTGCGCGAGCCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCcgacg                                                                                                                                 >  1:269830/1‑135 (MQ=255)    ccggcGAAGGCAAGACCACGACCACCGTGGGCCTGGCCGACGGCCTCAACCGCATCGGCAAGAAGACCGTGGCGGCGCTGCGCGAGCCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCcgacggcg                                                                                                                              <  8:76465/135‑1 (MQ=255)    ccggcGAAGGCAAGACCACGACCACCGTGGGCCTGGCCGACGGCCTCAACCGCATCGGCAAGAAGACCGTGGCGGCGCTGCGCGAGCCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCcgacggcg                                                                                                                              <  2:241755/135‑1 (MQ=255)          aaGGCAAGACCACGCCCACCGTGGGCCTGGCCGACGGCCTCAACCGCATCGGCAAGAAGACCGTGGCGGCGCTGCGCGAGCCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGACGGCGGCTATg                                                                                                                        <  8:21478/135‑1 (MQ=255)              cAAGACCACGACCACCGTGGGCCTGGCCGACGGCCTCAACCGCATCGGCAAGAAGACCGTGGCGGCGCTGCGCGAGCCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGACGGCGGCTATGCGCa                                                                                                                    <  7:202359/135‑1 (MQ=255)                        accaccGTGGGCCTGGCCGACGGCCTCAACCGCATCGGCAAGAAGACCGTGGCGGCTCTGCGCGAGCCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGACGGCGGCTATGCGCAGGTCGTGCCg                                                                                                          <  6:65224/135‑1 (MQ=255)                          caccGTGGGCCTGGCCGACGGCCTCAACCGCATCTGCAAGAAGACCGTTGCGGCGCTGCGCGAGCCTTCGCTCGGCCCCGGTTTCGGCGTGAAGGGCGGCGCGGCCGACGGCGGCTATGAGCAGGTCGTGCCGAt                                                                                                        >  7:323053/1‑135 (MQ=255)                          caccGTGGGCCTGGCCGACGGCCTCAACCGCATCGGCAAGAAGACCGTGGCGGCGCTGCGCGAGCCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGACGGCGGCTATGCGCAGGTCGTGCCGAt                                                                                                        >  5:336552/1‑135 (MQ=255)                          caccGTGGGCCTGGCCGACGGCCTCAACCGCATCGGCAAGAAGACCGTGGCGGCGCTGCGCGAGCCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGACGGCGGCTATGCGCAGGTCGTGCCGAt                                                                                                        >  5:297357/1‑135 (MQ=255)                           accGTGGGCCTGGCCGACGGCCTCAACCGCATCGGCAAGAAGACCGTGGCGGCGCTGCGCGAGCCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGACGGCGGCTATGCGCAGGTCGTGCCGATg                                                                                                       <  3:250380/135‑1 (MQ=255)                             cGTGGGCCTGGCCGACGGCCTCAACCGCATCGGCAAGAAGACCGTGGCGGCGCTGCGCGAGCCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGACGGCGGCTATGCGCAGGTCGTGCCGATgga                                                                                                     <  7:210500/135‑1 (MQ=255)                                gggCCTGGCCGACGGCCTCAACCGCAACGGCAAGAAGACCGTGGCGGCGCTGAGCGCGCCTTCGCTCGTCCCCTGTATCCGCCTGACGGCCGGCGCGGCCGACCCCCGCAATGCGCAGGACTAGCCGATggagg                                                                                                   >  8:79164/1‑134 (MQ=255)                                    cTGGCCGACGGCCTCAACCGCATCGGCAAGATGAGCGTGGCGGCGCTGCGCGAGCCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGACGGCGGCTATGCGCAGGTCGTGCCGATGGAGGACATc                                                                                              <  8:104861/135‑1 (MQ=255)                                          gACGGCCTCAACCGCATCGGCAAGAAGACCGTGGCGGCGCTGCGCGAGCCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGACGGCGGCTATGCGCAGGTCGTGCCGATGGAGGACATCAACct                                                                                         >  6:128075/1‑134 (MQ=255)                                              gCCTCAACCGCATCGGCAAGAAGACCGTGGCGGCGCTGCGCGAGCCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGACGGCGGCTATGCGCAGGTCGTGCCGATGGAGGACATCAACcttcact                                                                                    <  1:113396/135‑1 (MQ=255)                                                         aTCGGCAAGAAGACCGTGGCGGCGCTGCGCGAGCCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGACGGCGGCTATGCGCAGGTCGTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGAc                                                                         <  8:133950/135‑1 (MQ=255)                                                          tCGGCAAGAAGACCGTGGCGGCGCTGCGCGAGCCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGACGGCGGCTATGCGCAGGTCGTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGACt                                                                        <  1:236199/135‑1 (MQ=255)                                                           cGGCAAGAAGACCGTGGCGGCGCTGCGCGAGCCTTCGCTCGGCCGCTGTTTCGGCGTGAAGGGCGGCGCGGCCGACGGCGGCTATGCGCAGGTCGTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGACtt                                                                       >  4:21361/1‑135 (MQ=255)                                                                                    cacgAGCCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGACGGCGGCTATGCGCAGGTCGTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGACTTCCATGCCATCACCTCGGCGaacaac                                              >  5:86410/3‑135 (MQ=255)                                                                                           cTTCGCGCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGACGGCGGCTATGCGCAGGTCGTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGACTTCCATGCCATCACCTCGGCGAACAACCTGCTcg                                       <  1:321190/135‑1 (MQ=255)                                                                                               gCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGACGGCGGCTATGCGCAGGTCGTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGACTTCCATGCCATCACCTCGGCGAACAACCTGCTcgccg                                    <  8:258256/134‑1 (MQ=255)                                                                                               gCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGACGGCGGCTATGCGCAGGTCGTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGACTTCCATGCCATCACCTCGGCGAACAACCTGCTcgcc                                     <  6:336552/133‑1 (MQ=255)                                                                                                     ccccTGTTTCGGCGTGAAGGGCGGCGCGGCCGACGGCGGCTATGCGCAGGTCGTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGACTTCCATGCCATCACCTCGGCGAACAACCTGCTCGCCGCGCTGAt                             <  5:268234/135‑1 (MQ=255)                                                                                                       ccTGTTTCGGCGTGAAGGGCGGCGCGGCCGACGGCGGCTATGCGCAGGTCGTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGACTTCCATGCCATCACCTCGGCGAACAACCTGCTCGCCGCGCTGATCg                           <  6:181577/135‑1 (MQ=255)                                                                                                       ccTGTTTCGGCGTGAAGGGCGGCGCGGCCGACGGCGGCTATGCGCAGGTCGTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGACTTCCATGCCATCACCTCGGCGAACAACCTGCTCGCCGCGCTGATCg                           <  7:148560/135‑1 (MQ=255)                                                                                                        cTGTTTCGGCGTGAAGGGCGGCGCGGCCGACGGCGGCTATGCGCAGGTCGTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGACTTCCATGCCATCACCTCGGCGAACAACCTGCTCGCCGCGCTGATCg                           >  4:54646/1‑134 (MQ=255)                                                                                                        cTGTTTCGGCGTGAAGGGCGGCGCGGCCGACGGCGGCTATGCGCAGGTCGTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGACTTCCATGCCATCACCTCGGCGAACAACCTGCTCGCCGCGCTGATCg                           >  4:266427/1‑134 (MQ=255)                                                                                                         tttttCGGCGTGAAGGGCGGCTCGGCCGACGGCGGCTATGCGCAGTTCGTGCCGATGGAGGACATCAACCTTCACTTCACCGTTGACTTCCATGCCATCACATCGGCGAACAACCTTCTCGCCGCGCTGATCGAc                         <  8:250213/133‑1 (MQ=255)                                                                                                         tGTTTCGGCGTGAAGGGCGGCGCGGCCGACGGCGGCTATGCGCAGGTCGTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGACTTTCATGCCATCACCTCGGCGAACAACCTGCTCGCCGCGCTGATCGAc                         <  3:345736/135‑1 (MQ=255)                                                                                                            ttCGGCGTGAAGGGCGGCGCGGCCGACGGCGGCTATGCGCAGGTCGTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGACTTCCATGCCATCACCTCGGCGAACAACCTGCTCGCCGCGCTGATCTACAAc                      <  4:202373/135‑1 (MQ=255)                                                                                                            ttCGGCGTGAAGGGCGGCGCGGCCGACGGCGGCTATGCGCAGGTCGTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGACTTCCATGCCATCACCTCGGCGAACAACCTGCTCGCCGCGCTGATCGACAAc                      <  1:362054/135‑1 (MQ=255)                                                                                                                  gTGAAGGGCGGCGCGGCCGACGGCGGCTATGCGCAGGTCGTGCCGATGGAGGACATCAACCTTCACTTCACCTGTGACTTCCATGCCATCACCTCGGCGAACAACCTGCTCGCCGCGCTGATCGACAACCACAt                 >  1:313419/1‑134 (MQ=255)                                                                                                                  gTGAAGGGCGGCGCGGCCGACGGCGGCTATGCGCAGGTCGTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGACTTCCATGCCATCACCTCGGCGAACAACCTGCTCGCCGCGCTGATCGACAACCACAt                 >  3:11689/1‑134 (MQ=255)                                                                                                                     aaGGGCGGCGCGGCCGACGGCGGCTATGCGCAGGTCGTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGACTTCCATGCCATCACCTCGGCGAACAACCTGCTCGCCGCGCTGATCGACAACCACATCTAc             <  8:342132/135‑1 (MQ=255)                                                                                                                      aGGGCGGCGCGGCCGACGGCGGCTATGCGCAGGTCGTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGACTTCCATGCCATCACCTCGGCGAACAACCTGCTCGCCGCGCTGATCGACAACCACATCTAc             <  2:322668/134‑1 (MQ=255)                                                                                                                             cgcggcCGACGGCGGCTATGCGCAGGTCGTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGACTTCCATGCCATCACCTCGGCGAACAACCTGCTCGCCGCGCTGATCGACAACCACATCTACTGGGGca      >  3:310113/1‑134 (MQ=255)                                                                                                                                ggccgacGGCGGCTATGCGCAGGTCGTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGACTTCCATGCCATCACCTCGGCGAACAACCTGCTCGCCGCGCTGATCGACAACCACATCTACTGGGGcaacaa  <  8:238965/135‑1 (MQ=255)                                                                                                                                     |                                                                                                                                  CGGCCGGCGAAGGCAAGACCACGACCACCGTGGGCCTGGCCGACGGCCTCAACCGCATCGGCAAGAAGACCGTGGCGGCGCTGCGCGAGCCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAGGTCGTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGACTTCCATGCCATCACCTCGGCGAACAACCTGCTCGCCGCGCTGATCGACAACCACATCTACTGGGGCAACAA  >  ACB143/5390109‑5390370

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑