Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	ACB143	1,311,935	G→T	D36Y (GAT→TAT)	aaeA_2 →	p‑hydroxybenzoic acid efflux pump subunit AaeA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB143	1,311,935	0	G	T	100.0%	241.9 / NA	65	D36Y (GAT→TAT)	aaeA_2	p‑hydroxybenzoic acid efflux pump subunit AaeA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (41/24);  total (41/24)

AAAGGAGTGTTTCGATGCGTGCGGCCGTACGTATTCTGGTGACCCTGTGTGTGGTGGCCCTGGCCGTGTTGGCCGGCTACCAGCTGTGGCAGTACTACATGCTCACCCCATGGACGCGTGATGCCCGTGTGCGCGCCGATGTGGTGGTGATTGCGCCTGATGTGTCCGGCTGGGTTCGCGAACTCAAGGTCCATGACAACCAGCAGGTCAAGGCTGGCGACTTGTTGATGAGTATTGACCGCGAGCGC  >  ACB143/1311816‑1312063                                                                                                                        |                                                                                                                                 aaaGGAGTGTTTCGATGCGTGCGGCCGTACGTATTCTGGTGACCCTGTGTGTGGTGGCCCTGGCCGTGTTGGCCGGCTACCAGCTGTGGCAGTACTACATGCTCACCCCATGGACGCGTTATGCCCGTGTgcgcg                                                                                                                   >  6:354693/1‑135 (MQ=255) aaaGGAGTGTTTCGATGCGTGCGGCCGTACGTATTCTGGTGACCCTGTGTGTGGTGGCCCTGGCCGTGTTGGCCGGCTACCAGCTGTGGCAGTACTACATGCTCACCCCATGGACGCGTTATGCCCGTGTgcgcg                                                                                                                   >  4:349331/1‑135 (MQ=255) aaaGGAGTGTTTCGATGCGTGCGGACGTACGTATTCTGGTGACCCTGTGTGTGGTGGCCCTGGCCGTGTTGGCCGGCTACCAGCTGTGGCAGTACTACATGCTCACCCCATGGACGCGTTATGCCCGTGTgcgcg                                                                                                                   >  6:334699/1‑135 (MQ=255)         gtTTCGATGCGTGCGGCCGTACGTATTCTGGTGACCCTGTGTGTGGTGGCCCTGGCCGTGTTGGCCGGCTACCAGCTGTGGCAGTACTACATGCTCACCCCATGGACGCGTTATGCCCGTGTGCGCGCCGAtgtg                                                                                                           <  3:142346/135‑1 (MQ=255)           ttCGATGCGTGCGGCCGTACGTATTCTGGTGACCCTGTGTGTGGTGGCCCTGGCCGTGTTGGCCGGCTACCAGCTGTGGCAGTACTACATGCTCACCCCATGGACGCGTTATGCCCGTGTGCGCGCCGATgtggt                                                                                                         >  4:341673/1‑135 (MQ=255)             cGATGCGTGCGGCCGTACGTATTCTGGTGACCCTGTGTGTGGTGGCCCTGGCCGTGTTGGCCGGCTACCAGCTGTGGCAGTACTACATGCTCACCCCATGGACGCGTTATGCCCGTGTGCGCGCCGATgtggtgg                                                                                                       >  5:67819/1‑135 (MQ=255)             cGATGCGTGCGGCCGTACGTATTCTGGTGACCCTGTGTGTGGTGGCCCTGGCCGTGTTGGCCGGCTACCAGCTGTGGCAGTACTACATGCTCACCCCATGGACGCGTTATGCCCGTGTGCGCGCCGATgtggtgg                                                                                                       >  3:183434/1‑135 (MQ=255)             cGATGCGTGCGGCCGTACGTATTCTGGTGACCCTGTGTGTGGTGGCCCTGGCCGTGTTGGCCGGCTACCAGCTGTGGCAGTACTACATGCTCACCCCATGGACGCGTTATGCCCGTGTGCGCGCCGATgtggtgg                                                                                                       >  6:342878/1‑135 (MQ=255)               aTGCGTGCGGCCGTACGTATTCTGGTGACCCTGTGTGTGGTGGCCCTGGCCGTGTTGGCCGGCTACCAGCTGTGGCAGTACTACATGCTCACCCCATGGACGCGTTATGCCCGTGTGCGCGCCGATgtggtggt                                                                                                      <  4:295544/134‑1 (MQ=255)               aTGCGTGCGGCCGTACGTATTCTGGTGACCCTGTGTGTGGTGGCCCTGGCCGTGTTGGCCGGCTACCAGCTGTGGCAGTACTACATGCTCACCCCATGGACGCGTTATGCCCGTGTGCGCGCCGATgtggtggt                                                                                                      <  1:125695/134‑1 (MQ=255)                  cgtgcgGACGTACGTATTCTGGTGACCCTGTGTGTGGTGGCCCTGGCCGTGTTGGCCGGCTACCAGCTGTGGCAGTACTACATGCTCACCCCATGGACGCGTTATGCCCGTGTGCGCGCCGATGTGGTGGTGAtt                                                                                                  >  8:40367/1‑135 (MQ=255)                     gcgGCCGTACGTATTCTGGTGACCCTGTGTGTGGTGGCCCTGGCCGTGTTGGCCGGCTACCAGCTGTGGCAGTACTACATGCTCACCCCATGGACGCGTTATGCCCGTGTGCGCGCCGATGTGGTGGTGATTgcg                                                                                               >  5:151056/1‑135 (MQ=255)                       ggCCGTACGTATTCTGGTGACCCTGTGTGTGGTGGCCCTGGCCGTGTTGGCCGGCTACCAGCTGTGGCAGTACTACATGCTCACCCCATGGACGCGTTATGCCCGTGTGCGCGCCGATGTGGTGGTGATTGCGcc                                                                                             >  3:311465/1‑135 (MQ=255)                            tacgtaTTCTGGTGACCCTGTGTGTGGTGGCCCTGGCCGTGTTGGCCGGCTACCAGCTGTGGCAGTACTACATGCTCACCCCATGGACGCGTTATGCCCGTGTGCGCGCCGATGTGGTGGTGATTGCGCCTGAtg                                                                                        <  4:354537/135‑1 (MQ=255)                                      ggTGACCCTGTGTGTGGTGGCCCTGGCCGTGTTGGCCGGCTACCAGCTGTGGCAGTACTACATGCTCACCCCATGGACGCGTTATGCCCGTGTGCGCGCCGATGTGGTGGTGATTGCGCCTGATGTGTCCGGCTg                                                                              >  7:87536/1‑135 (MQ=255)                                         gACCCTGTGTGTGGTGGCCCTGGCCGTGTTGGCCGGCTACCAGCTGTGGCAGTACTACATGCTCACCCCATGGACGCGTTATGCCCGTGTGCGCGCCGATGTGGTGGTGATTGCGCCTGATGTGTCCGGCTGGGt                                                                           <  6:276563/135‑1 (MQ=255)                                         gACCCTGTGTGTGGTGGCCCTGGCCGTGTTGGCCGGCTACCAGCTGTGGCAGTACTACATGCTCACCCCATGGACGCGTTATGCCCGTGTGCGCGCCGATGTGGTGGTGATTGCGCCTGATGTGTCCGGCTGGGt                                                                           >  4:311921/1‑135 (MQ=255)                                          aCCCTGTGTGTGGTGGCCCTGGCCGTGTTGGCCGGCTACCAGCTGTGGCAGTACTACATGCTCACCCCATGGACGCGTTATGCCCGTGTGCGCGCCGATGTGGTGGTGATTGCGCCTGATGTGTCCGGCTGGGtt                                                                          >  5:300935/1‑135 (MQ=255)                                           cccTGTGTGTGGTGGCCCTGGCCGTGTTGGCCGGCTACCAGCTGTGGCAGTACTACATGCTCACCCCATGGACGCGTTATGCCCGTGTGCGCGCCGATGTGGGGGTGATTGCGCCTGATGTGTCCGGCTGGGTTc                                                                         >  7:137016/1‑135 (MQ=255)                                             cTGTGTGTGGTGGCCCTGGCCGTGTTGGCCGGCTACCAGCTGTGGCAGTACTACATGCTCACCCCATGGACGCGTTATGCCCGTGTGCGCGCCGATGTGGTGGTGATTGCGCCTGATGTGTCCGGCTGGGTTcg                                                                        >  1:247802/1‑134 (MQ=255)                                                 gtgtgGTGGCCCTGGCCGTGTTGGCCGGCTACCAGCTGTGGCAGTACTACATGCTCACCCCATGGACGCGTTATGCCCGTGTGCGCGCCGATGTGGTGGTGATTGCGCCTGATGTGTCCGGCTGGGTTCGCGAAc                                                                   <  2:305381/135‑1 (MQ=255)                                                 gtgtgGTGGCCCTGGCCGTGTTGGCCGGCTACCAGCTGTGGCAGTACTACATGCTCACCCCATGGACGCGTTATGCCCGTGTGCGCGCCGATGTGGTGGTGATTGCGCCTGATGTGTCCGGCTGGGTTCGCGAAc                                                                   <  1:92840/135‑1 (MQ=255)                                                 gtgtgGTGGCCCTGGCCGTGTTGGCCGGCTACCAGCTGTGGCAGTACTACATGCTCACCCCATGGACGCGTTATGCCCGTGTGCGCGCCGATGTGGTGGTGATTGCGCCTGATGTGTCCGGCTGGGTTCGCGAAc                                                                   <  4:59127/135‑1 (MQ=255)                                                 gtgtgGTGGCCCTGGCCGTGTTGGCCGGCTACCAGCTGTGGCAGTACTACATGCTCACCCCATGGACGCGTTATGCCCGTGTGCGCGCCGATGTGGTGGTGATTGCGCCTGATGTGTCCGGCTGGGTTCGCGAAc                                                                   <  1:257496/135‑1 (MQ=255)                                                   gtggttGCCCTGGCCGTGTTGGCCGGCTACCAGCTGTGTCAGTACTACATGCTCACCCCATGGACGCGTTATGCCCGTGTGCGCGCCGATGTGGTGGTGATTGCGCCTGATGTGTCCGGCGGGGTTCGCGAAATc                                                                 >  6:52422/1‑135 (MQ=255)                                                    tggtggCCCTGGCCGTGTTGGCCGGCTACCAGCTGTGGCAGTACTACATGCTCACCCCATGGACGCGTTATGCCCGTGTGCGCCCCGATGTGGTGGTGATTGCGCCTGATGTGTCCGGCTGGGTTCGCGCACt                                                                  >  5:44742/1‑133 (MQ=255)                                                        ggCCCTGGCCGTGTTGGCCGGCTACCAGCTGTGGCAGTACTACATGCTCACCCCATGGACGCGTTATGCCCGTGTGCGCGCCGATGTGGTGGTGATTGCGCCTGATGTGTCCGGCTGGGTTCGCGAACTCAAGGt                                                            <  4:173462/135‑1 (MQ=255)                                                         gCCCTGGCCGTGTTGGCCGGCTACCAGCTGTGGCAGTACTACATGCTCACCCCATGGACGCGTTATTCCCGTGTGCGCGCCGATGTGGTGGTGATTGCGCCTGATGTGTCCGGCTGGGTTCGCGAACTCAAGGt                                                            >  7:121509/1‑134 (MQ=255)                                                         gCCCTGGCCGTGTTGGCCGGCTACCAGCTGTGGCAGTACTACATGCTCACCCCATGGACGCGTTATGCCCGTGTGCGCGCCGATGTGGTGGTGATTGCGCCt                                                                                            <  8:88796/102‑1 (MQ=255)                                                         gCCCTGGCCGTGTTGGCCGGCTACCAGCTGTGGCAGTACTACATGCTCACCCCATGGACGCGTTATGCCCGTGTGCGCGCCGATGTGGTGGTGATTGCGCCt                                                                                            >  7:88796/1‑102 (MQ=255)                                                         gCCCTGGCCGTGTTGGCCGGCTACCAGCTGTGGCAGTACTACATGCTCACCCCATGGACGCGTTATGCCCGTGTGCGCGCCGATGTGGTGGTGATTGCGCCTGATGTGTCCGGCTGGGTTCGCGAACTCAAGGt                                                            >  5:145336/1‑134 (MQ=255)                                                         gCCCTGGCCGTGTTGGCCGGCTACCAGCTGTGGCAGTACTACATGCTCACCCCATGGACGCGTTATGCCCGTGTGCGCGCCGATGTGGTGGTGATTGCGCCTGAGGTGTCCGGCTGGGTTCGCGAACTCAAGGt                                                            >  7:21327/1‑134 (MQ=255)                                                             tGGCCGTGGTGGCCGGCTACCAGCTGTGGCAGTACTACATGCTCACCCCATGGACGCGTTATGCCCGTGTGCGCGCCGATGTGGTGGTGATTGCGCCTGATGTGTCCGGCTGGGTTCGCGAACTCAAGGTCCATg                                                       <  4:51018/135‑1 (MQ=255)                                                               gCCGTGTTGGCCGGCTACCAGCTGTGGCAGTACTACATGCTCACCCCATGGACGCGTTATGCCCGTGTGCGCGCCGATGTGGTGGTGATTGCGCCTGATGTGTCCGGCTGGGTTCGCGAACTCAAGGTCCATGa                                                      >  7:68179/1‑134 (MQ=255)                                                               gCCGTGTTGGCCGGCTACCAGCTGTGGCAGTACTACATGCTCACCCCATGGACGCGTTATGCCCGTGTGCGCGCCGATGTGGTGGTGATTGCGCCTGATGTGTCCGGCTGGGTTCGCGAACTCAAGGTCCATGa                                                      >  7:63120/1‑134 (MQ=255)                                                                 cGTGTTGGCCGGCTACCAGCTGTGGCAGTACTACATGCTCACCCCATGGACGCGTTATGCCCGTGTGCGCGCCGATGTGGTGGTGATTGCGCCTGATGTGTCCGGCTGGGTTCGCGAACTCAAGGTCCATGACaa                                                   >  4:300495/1‑135 (MQ=255)                                                                 cGTGTTGGCCGGCTACCAGCTGTGGCAGTACTACATGCTCACCCCATGGACGCGTTATGCCCGTGTGCGCGCCGATGTGGTGGTGATTGCGCCTGATGTGTCCGGCTGGGTTCGCGAACTCAAGGTCCATGACaa                                                   >  4:173595/1‑135 (MQ=255)                                                                       ggCCGGCTACCAGCTGTGGCAGTACTACATGCTCACCCCATGGACGCGTTATGCCCGTGTGCGCGCCGATGTGGTGGTGATTGCGCCTGATGTGTCCGGCTGGGTTCGCGAACTCAAGGTCCATGACAACcagca                                             >  6:21029/1‑135 (MQ=255)                                                                                  aGCTGTGGCAGTACTACATGCTCACCCCATGGACGCGTTATGCCCGTGTGCGCGCCGATGTGGTGGTGATTGCGCCTGATGTGTCCGGCTGGGTTCGCGAACTCAAGGTCCATGACAACCAGCAGGTCAAGGCTg                                  <  3:166793/135‑1 (MQ=255)                                                                                        ggCAGTACTACATGCTCACCCCATGGACGCGTTATGCCCGTGTGCGCGCCGATGTGGTGGTGATTGCGCCTGATGTGTCCGGCTGGGTTCGCGAACTCAAGGGCCATGACAACCAGCAGGTCAAGGCTGGCGAc                             <  8:287252/134‑1 (MQ=255)                                                                                           aGTACTACATGCTCACCCCATGGACGCGTTATGCCCGTGTGCGCGCCGATGTGGTGGTGATTGCGCCTGATGTGTCCGGCTGGGTTCGCGAACTCAAGGTCCATGACAACCAGCAGGTCAAGGCTGGCGACttgt                         <  2:243823/135‑1 (MQ=255)                                                                                            gTACTACATGCTCACCCCCTGGACGCGTTATGCCCGTGTGCGCGCCGATGTGGTGGTGATTGCGCCTGATGTGTCCGGCTGGGTTCGCGAACTCAAGGTCCATGACAACCAGCAGGTCAAGGCTGGCGACttgtt                        <  3:30021/135‑1 (MQ=255)                                                                                            gTACTACATGCTCACCCCCTGGACGCGTTATGCCCGTGTGCGCGCCGATGTGGTGGTGATTGCGCCTGATGTGTCCGGCTGGGTTCGCGAACTCAAGGTCCATGACAACCAGCAGGTCAAGGCTGGCGACttgtt                        <  8:104783/135‑1 (MQ=255)                                                                                            gTACTACATGCTCACCCCATGGACGCGTTATGCCCGTGTGCGCGCCGATGTGGTGGTGATTGCGCCTGATGTGTCCGGCTGGGTTCGCGAACTCAAGGTCCATGACAACCAGCAGGTCAAGGCTGGCGACttgtt                        <  4:82037/135‑1 (MQ=255)                                                                                            gTACTACATGCTCACCCCATGGACGCGTTATGCCCGTGTGCGCGCCGATGTGGTGGGGATTGCGCCTGATGTGTCCGGCTGGGTTCGCGAACTCAAGGTCCATGACAACCAGCAGGTCAAGGCTGGCGACttgtt                        <  3:195297/135‑1 (MQ=255)                                                                                                tacATGCTCACCCCATGGACGCGTTATGCCCGTGTGCGCGCCGATGTGGTGGTGATTGCGCCTGATGTGTCCGGCTGGGTTCGCGAACTCAAGGTCCATGACAACCAGCAGGTCAAGGCTGGCGACTTGTtga                      >  6:161077/1‑133 (MQ=255)                                                                                                tacATGCTCACCCCATGGACGCGTTATGCCCGTGTGCGCGCCGATGTGGTGGTGATTGCGCCTGATGTGTCCGGCTGGGTTCGCGAACTCAAGGTCCATGACAACCAGCAGGTCAAGGCTGGCGACTTGTtga                      >  2:256300/1‑133 (MQ=255)                                                                                                tacATGCTCACCCCATGGACGCGTTATGACCGTGTGCGCGCCGATGTGGTGGTGATTGCGCCCGATGTGTCCGGCTGGGTTCGCGAACTCAAGGTCCATGACACCCAGCAGGTCAAGGCTGGCGACTTGTtga                      >  5:195422/1‑133 (MQ=255)                                                                                                  cATGCTCACCCCATGGACGCGTTATGCCCGTGTGCGCGCCGATGTGGTGGTGATTGCGCCTGATGTGTCCGGCTGGGTTCGCGAACTCAAGGTCCATGACAACCAGCAGGTCAAGGCTGGCGACTTGTTGATGAg                  >  6:327526/1‑135 (MQ=255)                                                                                                    tGCTCACCCCATGGACGCGTTATGCCCGTGTGCGCGCCGATGTGGTGGTGATTGCGCCTGATGTGTCCGGCTGGGTTCGCGAACTCAAGGTCCATGACAACCAGCAGGTCAAGGCTGGCGACTTGGTGATGAGTa                >  8:134661/1‑135 (MQ=255)                                                                                                        cACCCCATGGACGCGTTATGCCCGTGTGCGCGCCGATGTGGTGGTGATTGCGCCTGATGTGTCAGGCTGGGTTCGCGAACTCAAGGTCCATGACAACCAGCAGGTGAAGGCTGGCGACTTGTTGATGAGTATTGa            >  8:188497/1‑135 (MQ=255)                                                                                                          ccccATGGACGCGTTATGCCCGTGTGCGCGCCGATGTGGTGGTGATTGCGCCTGATGTGTCCGGCTGGGTTCGCGAACTCAAGGTCCATGACAACCAGCAGGTCAAGGCTGGCGACTTGTTGATGAGTATTGAcc          >  1:15157/1‑135 (MQ=255)                                                                                                          ccccATGGACGCGTTATGCCCGTGTGCGCGCCGATGTGGTGGTGATTGCGCCTGATGTGTCCGGCTGGGTTCGCGAACTCAAGGTCCATGACAACCAGCAGGTCAAGGCTGGCGACTTGTTGATGAGTATTGAcc          >  8:252111/1‑135 (MQ=255)                                                                                                            ccATGGACGCGTTATGCCCGTGTGCGCGCCGATGTGGTGGTGATTGCGCCTGATGTGTCCGGCTGGGTTCGCGAACTCAAGGTCCATGACAACCAGCAGGTCAAGGCTGGCGACTTGTTGATGAGTATTGACcgc        <  7:253579/135‑1 (MQ=255)                                                                                                            ccATGGACGCGTTATGCCCGTGTGCGCGCCGATGTGGTGGTGATTGCGCCTGATGTGTCCGGCTGGGTTCGCGAACTCAAGGTCCATGACAACCAGCAGGTCAAGGCTGGCGACTTGTTGATGAGTATTGACcgc        <  8:124982/135‑1 (MQ=255)                                                                                                            ccATGGACGCGTTATGCCCGTGTGCGCGCCGATGTGGTGGTGATTGCGCCTGATGTGTCCGGCTGGGTTCGCGAACTCAAGGTCCATGACAACCAGCAGGTCAAGGCTGGCGACTTGTTGATGAGTATTGACcgc        <  5:21029/135‑1 (MQ=255)                                                                                                            ccATGGACGCGTTATGCCCGTGTGCGCGCCGATGTGGTGGTGATTGCGCCTGATGTGTCCGGCTGGGTTCGCGAACTCAAGGTCCATGACAACCAGCAGGTCAAGGCTGGCGACTTGTTGATGAGTATTGACcgc        >  3:178297/1‑135 (MQ=255)                                                                                                            ccATGGACGCGTTATGCCCGTGTGCGCGCCGATGTGGTGGTGATTGCGCCTGATGTGTCCGGCTGGGTTCGCGAACTCAAGGTCCATGACAACCAGCAGGTCAAGGCTGGCGACTTGTTGATGAGTATTGACcg         >  4:365959/1‑134 (MQ=255)                                                                                                            ccATGGACGCGTTATGCCCGTGTGCGCGCCGATGGGGTGGTGATTGCGCCTGTTGTGACCGGCTGGGTTCGCGAACTCAAGGTCCATGACAACCAGCAGGTCAAGGCTGGCGACTTGTTGATGAGTATTGACcgc        <  7:84709/135‑1 (MQ=255)                                                                                                             cATGGACGCGTTATGCCCGTGTGCGCGCCGATGTGGTGGTGATTGCGCCTGATGTGTCCGGCTGGGTTCGCGAACTCAAGGTCCATGACAACCAGCAGGTCAAGGCTGGCGACTTGTTGATGAGTATTGACcg         >  5:320185/1‑133 (MQ=255)                                                                                                                  aCGCGTTATGCCCGTGTGCGCGCCGATGTGGTGGTGATTGCGCCTGATGTGTCCGGCTGGGTTCGCGAAc                                                                   >  6:212667/1‑70 (MQ=255)                                                                                                                  aCGCGTTATGCCCGTGTGCGCGCCGATGTGGTGGTGATTGCGCCTGATGTGTCCGGCTGGGTTCGCGAAc                                                                   <  5:212667/70‑1 (MQ=255)                                                                                                                  aCGCGTTATGCCCGTGTGCGCGCCGATGTGGTGGTGATTGCGCCTGATGTGTCCGGCTGGGTTCGCGAACTCAAGGTCCATGACAACCAGCAGGTCAAGGCTGGCGACTTGTTTATGAGTATTGACAGCGAgcgc  >  7:6374/1‑135 (MQ=255)                                                                                                                  aCGCGTTATGCCCGTGTGCGCGCCGATGTGGTGGTGATTGCGCCTGATGTGTCCGGCTGGGTTCGCGAACTCAAGGTCCATGACAACCAGCAGGTCAAGGCTGGCGACTTGTTGATGAGTATTGACCGCGAgcgc  >  4:182817/1‑135 (MQ=255)                                                                                                                  aCGCGTTATGCCCGTGTGCGCGCCGATGTGGTGGTGATTGCGCCTGATGTGTCCGGCTGGGTTCGCGAACTCAAGGTCCATGACAACCAGCAGGTCAAGGCTGGCGACTTGTTGATGAGTATTGACCGCGAgcgc  >  3:127654/1‑135 (MQ=255)                                                                                                                        |                                                                                                                                 AAAGGAGTGTTTCGATGCGTGCGGCCGTACGTATTCTGGTGACCCTGTGTGTGGTGGCCCTGGCCGTGTTGGCCGGCTACCAGCTGTGGCAGTACTACATGCTCACCCCATGGACGCGTGATGCCCGTGTGCGCGCCGATGTGGTGGTGATTGCGCCTGATGTGTCCGGCTGGGTTCGCGAACTCAAGGTCCATGACAACCAGCAGGTCAAGGCTGGCGACTTGTTGATGAGTATTGACCGCGAGCGC  >  ACB143/1311816‑1312063

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑