Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	ACB143	4,627,056	C→T	G100D (GGC→GAC)	gacA ←	Response regulator GacA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB143	4,627,056	0	C	T	100.0%	163.6 / NA	48	G100D (GGC→GAC)	gacA	Response regulator GacA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/24);  total (24/24)

CCCCTGCGGCTGGAACGGTTTCAGCGCCAACTGCTGGGCGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGGTCAGGTAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCG  >  ACB143/4626929‑4627181                                                                                                                                |                                                                                                                              ccccTGCGGCTGGAACGGTTTCAGCGCCAACTGCTGGGCGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGGTCAGGTAGTCGGCAgc                                                                                                                        >  3:75582/1‑135 (MQ=255)   ccTGCGGCTGGAACGGTTTCAGCGCCAACTGCTGGGCGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGGTCAGGTAGTCGGCAgcgc                                                                                                                      <  1:265893/135‑1 (MQ=255)     tGCGGCTGGAACGGTTTCAGCGCCAACTGCTGGGCGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGGTCAGGTAGTCGGCAGCGCCg                                                                                                                    <  4:328630/135‑1 (MQ=255)        ggCTGGAACGGTTTCAGCGCCAACTGCTGGGCGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGGTCAGGTAGTCGGCAGCGCCGGcc                                                                                                                 >  4:69217/1‑135 (MQ=255)              aaCGGTTTCAGCGCCAACTGCTGGGCGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGGTCAGGTAGTCGGCAGCGCCGGCCTGcaac                                                                                                           <  7:210086/135‑1 (MQ=255)               aCGGTTTCAGCGCCAACTGCTGGGCGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGGTCAGGTAGTCGGCAGCGCCGGCCTGcaa                                                                                                            >  2:321641/1‑133 (MQ=255)                cGGTTTCAGCGCCAACTGCTGGGCGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGGTCAGGTAGTCGGCAGCGCCGGCCTGcaacaa                                                                                                         >  8:242292/1‑135 (MQ=255)                  gTTGCAGCGCCAACTGCTGGGCGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGGTCAGGTAGTCGGCAGCGCCGGCCTGCAACAAGc                                                                                                       <  5:105417/135‑1 (MQ=255)                         cgcCAACTGCTGGGCGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGGTCAGGTAGTCGGCAGCGCCGGCCTGCAACAAGCGTGTggg                                                                                                <  6:326028/135‑1 (MQ=255)                              aCTGCTGGGCGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGGTCAGGTAGTCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACg                                                                                           <  5:98840/135‑1 (MQ=255)                                    gggCGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGGTCAGGTAGTCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCtt                                                                                     >  5:180611/1‑135 (MQ=255)                                             gCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGGTCAGGTAGTCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGcaca                                                                            >  6:37298/1‑135 (MQ=255)                                             gCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGGTCAGGTAGTCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGcaca                                                                            >  5:129782/1‑135 (MQ=255)                                             gCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGGTCAGGTAGTCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGcaca                                                                            >  3:302427/1‑135 (MQ=255)                                                       ataGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGGTCAGGTAGTCGGCAGCGCCGGCCTGCAACACGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCCCCg                                                                   >  4:198508/1‑134 (MQ=255)                                                       ataGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGGTCAGGTAGTCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCg                                                                   <  7:84014/134‑1 (MQ=255)                                                       ataGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGGTCAGGTAGTCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCg                                                                   <  5:69185/134‑1 (MQ=255)                                                            gcTGGCCGGCAAAGGCCAGACGAATTGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGGTCAGGTAGTCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTa                                                             <  7:199298/135‑1 (MQ=255)                                                             cTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGGTCAGGTAGTCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTAc                                                            <  8:119862/135‑1 (MQ=255)                                                                 ccGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGGTCAGGTAGTCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCtt                                                         <  8:195528/134‑1 (MQ=255)                                                                   ggCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGGTCAGGTAGTCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGAt                                                      >  5:222864/1‑135 (MQ=255)                                                                   ggCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGGTCAGGTAGTCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGAt                                                      >  6:220933/1‑135 (MQ=255)                                                                   ggCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGGTCAGGTAGTCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTGGAt                                                      >  8:331713/1‑135 (MQ=255)                                                                   ggCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGGTCAGGTAGTCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCGTCCTCGCACACCGTCACCGCCACTACCTTGAt                                                      >  7:159049/1‑135 (MQ=255)                                                                   ggCAAAGGCCAGACGAATGGCCTGGACCATATCGACAAGGCCTGCACCCTTGGTCAGGTAGTCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTACTCGCACACCGTCACCGCCACTACCTGGAt                                                      >  7:50065/1‑135 (MQ=255)                                                                          gCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGGTCAGGTAGTCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAggg                                               <  8:213267/135‑1 (MQ=255)                                                                          gCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGGTCAGGTAGTCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAggg                                               <  8:211657/135‑1 (MQ=255)                                                                             aGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGGTCAGGTAGTCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTgg                                            >  4:329013/1‑135 (MQ=255)                                                                               aCGAATGGACTGGACCAATTCGGCAAGGCCTGCACCCTTGGTCAGGTAGTCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGGCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCt                                          <  5:269199/135‑1 (MQ=255)                                                                                  actGGCCGGGACCATTTCGTCAAGGCCTGCACCCTTGGTCAGGTAGTCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTgcg                                       <  8:215643/133‑1 (MQ=255)                                                                                  aaTGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGGTCAGGTAGTCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTgc                                        >  6:48545/1‑134 (MQ=255)                                                                                       ccTGGACCATTTCGTCAAGGCCTGCACCCTTGGTCAGGTAGTCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCgcagca                                  >  6:142392/1‑135 (MQ=255)                                                                                       ccTGGACCATTTCGTCAAGGCCTGCACCCTTGGTCAGGTAGTCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCgcagca                                  >  4:339702/1‑135 (MQ=255)                                                                                         tGGACCATTTCGTCAAGGCCTGCACCCTTGGTCAGGTAGTCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGt                                <  2:172027/135‑1 (MQ=255)                                                                                            aCCATTTCGTCAAGGCCTGCACCCTTGGTCAGGTAGTCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACCCCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTg                             >  5:35280/1‑135 (MQ=255)                                                                                            aCCATTTCGTCAAGGCCTGCACCCTTGGTCAGGTAGTCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTg                             <  6:46086/135‑1 (MQ=255)                                                                                            aCCATTTCGTCAAGGCCTGCACCCTTGGTCAGGTAGTCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTg                             <  2:331063/135‑1 (MQ=255)                                                                                              cttttCGTCAAGGCCTGCAACCTTTGTCAGGTAGTCGGCAGCGCCGGGCTGCATGAAGCGTGTGGGGAACTGGTCTTCCTCGCACAGCGTCACCGCCACTAGCTTGATGTCAGGGTGGCTTCGCAGGAGTTTGCg                           <  6:68748/133‑1 (MQ=255)                                                                                              cATTTCGTCAAGGCCTGCACCCTTGGTCAGGTAGTCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCg                           <  3:339702/135‑1 (MQ=255)                                                                                              cATTTCGTCAAGGCCTGCACCCTTGGTCAGGTAGTCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCg                           <  5:249484/135‑1 (MQ=255)                                                                                              cATTTCGTCAAGGCCTGCACCCTTGGTCAGGTAGTCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCg                           <  5:80548/135‑1 (MQ=255)                                                                                              cATTTCGGCAAGGCCTGCACCCTTGGTCAGGTAGTCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCg                           <  6:335488/135‑1 (MQ=255)                                                                                                   cGTCAAGGCCTGCACCCTTGGTTAGGTAGTCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTgg                      >  6:301624/1‑135 (MQ=255)                                                                                                   cGTCAAGGCCTGCACCCTTGGTCAGGTAGTCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTgg                      >  1:288448/1‑135 (MQ=255)                                                                                                    gTCAAGGCCTGCACCCTTGGTCAGGTAGTCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGc                     >  6:271347/1‑135 (MQ=255)                                                                                                        aGGCCTGCACCCTTGGTCAGGTAGTCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTcc                 <  7:329250/135‑1 (MQ=255)                                                                                                                       gTCAGGTAGTCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCCCTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCg  >  2:131133/1‑135 (MQ=255)                                                                                                                       gTCAGGTAGTCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCg  >  4:141468/1‑135 (MQ=255)                                                                                                                                |                                                                                                                              CCCCTGCGGCTGGAACGGTTTCAGCGCCAACTGCTGGGCGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGGTCAGGTAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCG  >  ACB143/4626929‑4627181

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑