Predicted mutation
evidence seq id position mutation annotation gene description
RA ACB143 44,301 (T)9→8 intergenic (+132/‑359) oprD_1 → / → czcR_2 Porin D/Transcriptional activator protein CzcR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB14344,2930T.98.5% 298.9 / ‑1.3 66intergenic (+124/‑367)oprD_1/czcR_2Porin D/Transcriptional activator protein CzcR
Reads supporting (aligned to +/- strand):  ref base T (1/0);  major base . (24/40);  minor base G (1/0);  total (26/40)
Fisher's exact test for biased strand distribution p-value = 3.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GATTCTGTAATTCGCTAACTGGGCCAGTTATCCATTGGCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAATGTGAGCGACCTCGCCGTCATCTGAGCGGAAC  >  ACB143/44160‑44415
                                                                                                                                     |                                                                                                                          
gATTCTGTAATTCGCTAACTGGGCCAGTTATCCATTGGCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCtttt                                                                                                                         >  4:327670/1‑137 (MQ=255)
gATTCTGTAATTCGCTAACTGGGCCAGTTATCCATTGGCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCtttt                                                                                                                         >  6:232910/1‑137 (MQ=255)
gATTCTGTAATTCGCTAACTGGGCCAGTTATCCATTGGCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCtttt                                                                                                                         >  2:384976/1‑137 (MQ=255)
      ttAATTCGCTAACTGGGCCTGTTATCCATTGGCGGGTCCTGTTACTAAAACGAGCATTTCGCATTCACCTTGAGTGTCTTAAGTTCTCCTTTGGTTTAAGGTGGATATTTGGGGCCCATTGGGCGCCttttttttgc                                                                                                                   <  4:179811/136‑3 (MQ=255)
        aaTTCGCTAACTGGGCCAGTTATCCATTGGCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCttttttttgcct                                                                                                                 >  1:377624/1‑133 (MQ=255)
        aaTTCGCTAACTGGGCCAGTTATCCATTGGCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCttttttttgcat                                                                                                                 >  8:205507/1‑133 (MQ=255)
         aTTCGCTAACTGGGCCAGTTATCCATTGGCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTa                                                                                                               <  1:154287/137‑1 (MQ=255)
             gCTAACTGGGCCAGTTATCCATTGGCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAAt                                                                                                           >  8:177079/1‑137 (MQ=255)
              cTAACTGGGCCAGTTATCCATTGGCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGTATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAAtt                                                                                                          <  1:191765/137‑1 (MQ=255)
               tAACTGGGCCAGTTATCCATTGGCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAAtta                                                                                                         >  4:339029/1‑136 (MQ=255)
                aaCTGGGCCAGTTATCCATTGGCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCa                                                                                                        <  6:412723/137‑1 (MQ=255)
                       ccAGTTATCCATTGGCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGc                                                                                                 <  5:1394/137‑1 (MQ=255)
                         aGTTATCCATTGGCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGctt                                                                                               >  7:283170/1‑135 (MQ=255)
                              tCCATTGGCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTTCCTAAAATTAAATTAGGCGTTAGAt                                                                                          >  6:396028/1‑137 (MQ=255)
                                cATTGGCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGAttt                                                                                        >  7:459304/1‑136 (MQ=255)
                                cATTGGCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTg                                                                                        >  7:5594/1‑137 (MQ=255)
                                 aTTGGCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGTTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGt                                                                                       <  7:340173/137‑1 (MQ=255)
                                 aTTGGCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGt                                                                                       <  7:205507/137‑1 (MQ=255)
                                    ggCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTAcg                                                                                    <  8:459304/137‑1 (MQ=255)
                                     gCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTAcgc                                                                                   <  4:182807/137‑1 (MQ=255)
                                           ccTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTAcgcatcgca                                                                             >  5:22210/1‑137 (MQ=255)
                                                tAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCg                                                                        <  3:114141/137‑1 (MQ=255)
                                                tAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCg                                                                        <  6:98152/137‑1 (MQ=255)
                                                tAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCg                                                                        <  3:327670/137‑1 (MQ=255)
                                                tAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCg                                                                        <  1:384976/137‑1 (MQ=255)
                                                tAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCg                                                                        <  4:300072/137‑1 (MQ=255)
                                                tAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCg                                                                        <  2:61094/137‑1 (MQ=255)
                                                       aCGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGt                                                                 >  5:88979/1‑137 (MQ=255)
                                                        cGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGtt                                                                <  5:6346/137‑1 (MQ=255)
                                                         gAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTc                                                               >  7:130104/1‑137 (MQ=255)
                                                          aGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCa                                                              >  4:417118/1‑137 (MQ=255)
                                                          aGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCa                                                              <  5:26241/137‑1 (MQ=255)
                                                           gCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCAt                                                             >  7:79673/1‑137 (MQ=255)
                                                            cATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATg                                                            >  7:86030/1‑137 (MQ=255)
                                                             aTTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGa                                                           <  1:486370/137‑1 (MQ=255)
                                                                 cGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTac                                                       <  2:12912/137‑1 (MQ=255)
                                                                 cGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTac                                                       <  5:311498/137‑1 (MQ=255)
                                                                      tCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCaa                                                  <  2:444018/137‑1 (MQ=255)
                                                                        aCCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATg                                                <  7:177079/137‑1 (MQ=255)
                                                                        aCCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATg                                                <  2:13264/137‑1 (MQ=255)
                                                                        aCCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATg                                                <  5:243080/137‑1 (MQ=255)
                                                                         ccTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGGCTAAAATTCAAGTAGGCGTTGGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATgt                                               >  3:341483/1‑136 (MQ=255)
                                                                         ccTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGGCTAAAATTCAAGTAGGCGTTCGATTGTAAGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGa                                               >  2:458419/1‑137 (MQ=255)
                                                                          cTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGAc                                              <  4:453330/137‑1 (MQ=255)
                                                                          cTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGAc                                              <  6:150238/137‑1 (MQ=255)
                                                                           ttGATTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGAcc                                             <  7:61604/137‑1 (MQ=255)
                                                                           ttGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGAcc                                             <  8:410602/137‑1 (MQ=255)
                                                                           ttGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGAcc                                             <  1:85765/137‑1 (MQ=255)
                                                                           ttGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTATTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGAcc                                             <  1:369111/137‑1 (MQ=255)
                                                                            tGAGGGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCa                                            <  5:396028/137‑1 (MQ=255)
                                                                             gAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAg                                           <  3:339029/137‑1 (MQ=255)
                                                                                tGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGtct                                        >  6:367646/1‑137 (MQ=255)
                                                                                tGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGtct                                        <  2:167331/137‑1 (MQ=255)
                                                                                tGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTAGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGtct                                        >  2:394201/1‑137 (MQ=255)
                                                                                    ttAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCGTTTTTTTTTCCTAAAATTAAAGTTGGCGTTGGATTGTTCGCATAGCATGCAGGTGAAGTTCATTTCTACACCAATGACCAggcacgt                                     >  5:71096/1‑131 (MQ=255)
                                                                                     tAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGtt                                                                                              <  8:226036/78‑1 (MQ=255)
                                                                                     tAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGtt                                                                                              >  7:226036/1‑78 (MQ=255)
                                                                                     tAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTAcgca                                                                                  <  1:470529/90‑1 (MQ=255)
                                                                                     tAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTAcgca                                                                                  >  2:470529/1‑90 (MQ=255)
                                                                                       aGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAAt                                 >  3:431147/1‑137 (MQ=255)
                                                                                         tGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAAtgt                               <  5:218050/137‑1 (MQ=255)
                                                                                             ccTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTAATGACTAAAACAATGACCAGTCTCTTCAATGTTAGc                           >  7:255211/1‑137 (MQ=255)
                                                                                              cTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAATGTGAGCg                          >  3:324887/1‑137 (MQ=255)
                                                                                               tttGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCg                                                                        <  1:217140/90‑1 (MQ=255)
                                                                                               tttGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCg                                                                        >  2:217140/1‑90 (MQ=255)
                                                                                               tttGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTAGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCGCTTAAATGTgagaga                         >  8:114671/1‑137 (MQ=255)
                                                                                                ttGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAATGTGAGCGAc                        >  7:251697/1‑137 (MQ=255)
                                                                                                   gTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAATGTGAGCGACCTc                     <  3:25959/137‑1 (MQ=255)
                                                                                                             gATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAATGTGAGCGACCTCGCCGTCATCt           <  3:306743/137‑1 (MQ=255)
                                                                                                              atatTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAATGTGAGCGACCTCGCCGTCATCTg          <  6:64382/137‑1 (MQ=255)
                                                                                                              atatTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAATGTGAGCGACCTCGCCGTCATCTg          <  6:39390/137‑1 (MQ=255)
                                                                                                                      cgcCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAATGTGAGCGACCTCGCCGTCATCTGAGCGGAAc  >  3:432412/1‑137 (MQ=255)
                                                                                                                                     |                                                                                                                          
GATTCTGTAATTCGCTAACTGGGCCAGTTATCCATTGGCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAATGTGAGCGACCTCGCCGTCATCTGAGCGGAAC  >  ACB143/44160‑44415

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: