Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB143916,6960TG31.4% 95.9 / 11.5 70Q516P (CAG→CCG) ltxBLeukotoxin export ATP‑binding protein LtxB
Reads supporting (aligned to +/- strand):  ref base T (9/39);  new base G (21/1);  total (30/40)
Fisher's exact test for biased strand distribution p-value = 6.73e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GGTCGAGGTGGGCCATGGCGATGCCGTCCAGGCTGATCTCGCCCTGCACCAGATCCATGGCCCCACCCAGCGCCTGCAGCAAGGTTGATTTGCCGGCGCCGTTGCGGCCCAGCACGGCAATGCGCTCGCCCGGCTGGATATCCAGCCGGCCAATGTTCAGCACTGGCGGGTAGTCTTCGCTGTAGCGGAAGTTGGCCTGGCGCAGGCGGTACTCGCCGTGAATGGCCGGCAGGTGCACGCGGGCTTCGCCTTCGGGGTGATCGACC  >  ACB143/916562‑916827
                                                                                                                                      |                                                                                                                                    
ggTCGAGGTGGGCCATGGCGATGCCGTCCAGGCTGATCTCGCCCTGCACCAGATCCATGGCCCCACCCAGCGCCTGCAGCAAGGTTGATTTGCCGGCGCCGTTGCGGCCCAGCACGGCAATGCGCTCGCCCGGCggg                                                                                                                                    >  6:162902/1‑137 (MQ=255)
ggTCGAGGTGGGCCATGGCGATGCCGTCCAGGCTGATCTCGCCCTGCACCAGATCCATGGCCCCACCCAGCGCCTGCAGCAAGGTTGATTTGCCGGCGCCGTTGCGGCCCAGCACGGCAATGCGCTCGCCCGGCTgg                                                                                                                                    <  3:298348/137‑1 (MQ=255)
ggTCGAGGTGGGCCATGGCGATGCCGTCCAGGCTGATCTCGCCCTGCACCAGATCCATGGCCCCACCCAGCGCCTGCAGCAAGGTTGATTTGCCGGCGCCGTTGCGGCCCAGCACGGCAATGCGCTCGCCCGGCTgg                                                                                                                                    <  3:304213/137‑1 (MQ=255)
   cGAGGTGGGCCATGGCGATGCCGTCCAGGCTGATCTCGCCCTGCACCAGATCCATGGCCCCACCCAGCGCCTGCAGCAAGGTTGATTTGCCGGCGCCGTTGCGGACCAGCACGGCAATGCGCTCGCCCGGCGGGatt                                                                                                                                 >  5:243567/1‑136 (MQ=255)
     cggTGGGCCAGGGCGATGCCGGCCAGGCTGCTCTCGCCCTGCACCCGATCCATGGCCCCACCCAGCGCCTGCAGCAAGGTTGATTTGCCGGCGCCTTTGCGGCCCAGCACGGCAATGCTCTCGCCCGGCTGGATATc                                                                                                                               <  6:339154/136‑1 (MQ=255)
     aGGTGGGCCATGGCGATGCCGTCCAGGCTGATCTCGCCCTGCACCAGATCCATTGCCCCACCCAGCGCCTGCAGCAAGGTTGATTTGCCGGCGCCGTTGCGGCCCAGCACGGCAATGCGCTCGCCCGGCTGGATATc                                                                                                                               <  4:429792/137‑1 (MQ=255)
     aGGTGGGCCATGGCGATGCCGTCCAGGCTGATCTCGCCCTGCACCAGATCCATGGCCCCACCCAGCGCCTGCAGCAAGGTTGATTTGCCGGCGCCGTTGCGGCCCAGCACGGCAATGCGCTCGCGCGGCGGGctatg                                                                                                                               >  5:488596/1‑136 (MQ=255)
     aGGTGGGCCATGGCGATGCCGTCCAGGCTGATCTCGCCCTGCACCAGATCCATGGCCCCACCCAGCGCCTGCAGCAAGGTTGATTTGCCGGCGCCGTTGCGGCCCAGCACGGCAATGCGCTCGCCCGGCTGGatata                                                                                                                               >  6:223829/1‑136 (MQ=255)
     aGGTGGGCCATGGCGATGCCGTCCAGGCTGATCTCGCCCTGCACCAGATCCATGGCCCCACCCAGCGCCTGCAGCAAGGTTGATTTGCCGGCGCCGTTGCGGCCCAGCACGGCAATGCGCTCGCCCGGCTGGATATc                                                                                                                               <  4:450011/137‑1 (MQ=255)
     aGGTGGGCCATGGCGATGCCGTCCAGGCTGATCTCGCCCTGCACCAGATCCATGGCCCCACCCAGCGCCTGCAGCAAGGTTGATTTGCCGGCGCCGTTGCGGCCCAGCACGGCAATGCGCTCGCCCGGCTGGATATc                                                                                                                               <  3:89981/137‑1 (MQ=255)
     aGGTGGGCCATGGCGATGCCGTCCAGGCTGATCTCGCCCTGCACCAGATCCATGGCCCCACCCAGCGACTGCAGCAAGGTTGATTTGCCGGCGCCGTTGCGGCCCAGCACGGCAATGCGCTCGCCCGGCGGGaaata                                                                                                                               >  7:79441/1‑136 (MQ=255)
          ggCCATGGCGATGCCGTCCAGGCTGATCTCGCCCTTCACCAGATCCATGGCCCCAGCCAGCGCCTGCAGCAAGGTTGATTTGCCGGCGCCGTTGCGGCCCAGCACGGCAATGCGCTCGCGCGGCTGGATATCCAGcc                                                                                                                         <  6:385254/137‑1 (MQ=255)
          ggCCATGGCGATGCCGTCCAGGCTGATCTCGCCCTGCACCAGATCCATGGCCCCACCCAGCGCCTGCAGCAAGGTTGATTTGCCGGCGCCGTTGCGGCCCAGCACGGCAATGCGCTCGCCCGGCTGGATATCCAGcc                                                                                                                         <  8:166771/137‑1 (MQ=255)
          ggCCATGGCGATGCCGTCCAGGCTGATCTCGCCCTGCACCAGATCCATGGCCCCACCCAGCGCCTGCAGCAAGGTTGATTTGCCGGCGCCGTTGCGGCCCAGCACGGCAATGCGCTCGCCCGGCTGGATATCCAGcc                                                                                                                         <  7:411343/137‑1 (MQ=255)
           gCCATGGCGATGCCGTCCAGGCTGATCTCGCCCTGCACCAGATCCATGGCCCCACCCAGCGCCTGCAGCAAGGTTGATTTGCCGGCGCCGTTGCGGCCCAGCACGGCAATGCGCTCGCCCGGCTGGATATCCAGCCg                                                                                                                        <  8:255067/137‑1 (MQ=255)
             cATGGCGATGCCGTCCAGGCTGATCTCGCCCTGCACCAGATCCATGGCCCCACCCAGCGCCTGCAGCAAGGTTGATTTGCCGGCGCCGTTGCGGCCCAGCACGGCAATGCGCTCGCCCGGCGGGATATACACCCGGc                                                                                                                      >  4:280135/1‑137 (MQ=255)
               tGGCGATGCCGTCCAGGCTGATCTCGCCCTGCACCAGATCCATGGCCCCACCCAGCGCCTGCAGCAAGGTTGATTTGCCGGCGCCGTTGCGGCCCAGCACGGCAATGCGCTCGCCCGGCGGGCTTTGCACCCGGCCa                                                                                                                    >  7:406624/1‑137 (MQ=255)
                 gCGATGCCGTCCAGGCTGATCTCGCCCTGCACCAGATCCATGGCCCCACCCAGCGCCTGCAGCAAGGTTGATTTGCCGGCGCCGTTGCGGCCCAGCACGGCAATGCGCTCGCCCGGCTGGATATCCAGCCGGCCAAt                                                                                                                  <  2:302768/137‑1 (MQ=255)
                  cGATGCCGTCCAGGCTGATCTCGCCCTGCACCAGATCCATGGCCCCACCCAGCGCCTGCAGCAAGGTTGATTTGCCGGCGCCGTTGCGGCCCAGCACGGCAATGCGCTCGCGCGGGGGGGTTTGCAGCCGGcccagg                                                                                                                 >  7:32228/1‑133 (MQ=255)
                  cGATGCCATCCAGGCTGATCTCGCCCTGCACCAGATCCATGGCCCCACCCAGCGCCTGCAGCAAGGTTGATTTGCCGGCGCCGTTGCGGCCCAGCACGGCAATGCGCTCGCCCGGCTGGGTTTACAGCCGGCCAAtt                                                                                                                 >  7:300609/1‑136 (MQ=255)
                    aTGCCGTCCAGGCTGATCTCGCCCTGCACCAGATCCATGGCCCCACCCAGCGCCTGCAGCAAGGTTGATTTGCCGGCGCCGTTGCGGCCCAGCACGGCAATGCGCTCGCCCGGCTGGATATCCAGCCGGCCAATGtt                                                                                                               <  6:270433/137‑1 (MQ=255)
                      gCCGTCCAGGCTGATCTCGCCCTGCACCAGATCCATGGCCCCACCCAGCGCCTGCAGCAAGGTTGATTTGCCGGCGCCGTTGCGGCCCAGCACGGCAATGCGCTCGCCCGGCTGGATATCCAGCCGGCCAATGTTCa                                                                                                             <  2:202813/137‑1 (MQ=255)
                       ccGTCCAGGCTGATCTCGCCCTGCACCAGATCCATGGCCCCACCCAGCGCCTGCAGCAAGGTTGATTTGCCGGCGCCGTTGCGGCCCAGCACGGCAATGCGCTCGCCCGGCGGGGGATACAGCCGGCCCATGTTCAg                                                                                                            >  1:301766/1‑137 (MQ=255)
                         gTCCAGGCTGATCTCGCCCTGCACCAGATCCATGGCCCCACCCAGCGCCTGCAGCAAGGTTGATTTGCCGGCGCCGTTGCGGCCCAGCCCGGCAATGCGCTCGCCCGGCGGGATATCCAGCCGGCCAATGTTCAGcc                                                                                                          >  8:385809/1‑136 (MQ=255)
                             aGGCTGATCTCGCCCTGCACCAGATCCATGGCCCCACCCAGCGCCTGCAGCAAGGTTGATTTGCCGGCGCCGTTGCGGCCCAGCACGGCAATGCGCTCGCCCGGCTGGATATCCACCCGGCCAAAGTTCCGCACTgg                                                                                                      >  2:263930/1‑137 (MQ=255)
                             aGGCTGATCTCGCCCTGCACCAGATCCATGGCCCCACCCAGCGCCTGCAGCAAGGTTGATTTGCCGGCGCCGTTGCGGCCCAGCACGGCAATGCGCTCGCCCGGCGGGATATACACCCCGCCCAAGTTAACCACAgg                                                                                                      >  6:148928/1‑137 (MQ=255)
                              ggCTGATCTCGCCCTGCACCAGATCCATGGCCCCACCCAGCGCCTGCAGCAAGGTTGATTTGCCGGCGCCGTTGCGGCCCAGCACGGCAATGCGCTCGCCCGGCTGGATATCCAGCCGGCCAATGTTCAGCACTGGc                                                                                                     <  4:435578/137‑1 (MQ=255)
                               gcggATCTCGCCCTGCACCAGATCCATGGCCCCACCCAGCGCCTGCAGCAAGGTTGATTTGCCGGCGCCGTTGCGGCCCAGCACGGCAATGCGCTCGCCCGGCTGGATATCCAGCCGGCCAATGTTCAGCACTGGCg                                                                                                    <  4:392563/134‑1 (MQ=255)
                               gCTGATCTCGCCCTGCACCATATCCATGGCCCCAGCCAGCTCCTGCAGCAAGGTGGATTTGCCGGCGCCGTTGCGGCCCTGCACGGCAATGCGCTCGCCCGGCTGGATATCCTGCCGGCCAATGTTCAGCACTGGCg                                                                                                    <  2:221974/137‑1 (MQ=255)
                                      tcGCCCTGCACCAGATCCATTGCCCCACCCAGCGCCTGCAGCAAGGTTGATTTGCCGGCGCCGTTGCGGCCCAGCACGGCAATGCGCTCGCCCGGGGTGCTATACACCCCGCCCAAGTTGAGCAAAGGCggggtgtg                                                                                             >  4:391768/1‑132 (MQ=255)
                                      tcGCCCTGCACCAGATCCATGGCCCCACCCAGCGCCTGCAGCAAGGTTGATTTGCCGGCGCCGTTGCGGCCCAGCACGGCAATGCGCTCGCCCGGCTGGGTATACAGCCGGCCAAAGTTGAGCACGGGCGGGTTGtg                                                                                             >  6:269422/1‑136 (MQ=255)
                                       cGCCCTGCACCAGATCCATGGCCCCACCCAGCGCCTGCAGCAAGGTTGATTTGCCGGCGCCGTTGCGGCCCAGCACGGCAATGCGCTCGCCCGGCGGGATATACACCCCGCCAATGTTGAGCACTGGCGGGGAGTCt                                                                                            >  6:238281/1‑137 (MQ=255)
                                        gCCCTGCACCAGATCCATGGCCCCACCCAGCGCGTGGAGCAAGTTTGATTTGCGGGCGCCGTTGCGGCCCAGCACGGCAATGCGCTCGCCCGGCTGGATATCGAGCCGGCCAATGTTCAGCACTGGCGGGTAGTCtt                                                                                           <  3:355195/137‑1 (MQ=255)
                                         cccTGCACCAGATCCATGGCCCCACCCAGCGCCTGCAGCAAGGTTGATTTGCCGGCGCCGTTGCGGCCCAGCACGGCAATGCGCTCGCCCGGCTGGATATCCAGCCGGCCAATGTTCAGCACTGGCGGGTAGTCTTc                                                                                          <  3:215996/137‑1 (MQ=255)
                                         cccTGCACCAGATCCATGGCCCCACCCAGCGCCTGCAGCAAGGTTGATTTGCCGGCGCCGTTGCGGCCCAGCACGGCAATGCGCTCGCCCGGCTGGATATCCAGCCGGCCAATGTTCAGCACTGGCGGGTAGTCTTc                                                                                          <  5:104748/137‑1 (MQ=255)
                                            tGCACCAGATCCATGGCCCCACCCAGCGCCTGCAGCAAGGTTGATTTGCCGGCGCCGTTGCGGCCCAGCACGGCAATGCGCTCGCCCCGCGGGGTATCCAGCCCGCCAAAGTTGAGCACGGGCGGGGTGTCTTAGCt                                                                                       >  7:23092/1‑137 (MQ=255)
                                             tcACCAGATCCATGGCCCCACCCAGCGCCTGCAGCAAGGTTGATTTGCCGGCGCCGTTGCGGCCCAGCACGGCAATGCGCTCGCCCGGCTGGATATCCAGCCGGCCAATGTTCAGCACTGGCGGGTAGTCTTCGCTg                                                                                      <  3:104615/136‑1 (MQ=255)
                                                ccAGATCCATGGCCCCACCCAGCGCCTGCAGCAAGGTTGATTTGCCGGCGCCGTTGCGGCCCAGCACGGCAATGCGCTCGCCCGGCGGGGTATCCACCCCGCCAAAGGTGAGCACGCGCGGGGTGTCTTCGCGGTAg                                                                                   >  8:54945/1‑137 (MQ=255)
                                                     tCCATGGCCCCACCCAGCGCCTGCAGCAAGGTTGATTTGCCGGCGCCGTTGCGGCCCAGCACGGCAATGCGCTCGCCCGGCGGGATAAACAGCCGGGCAATGTTCAGCACAGGGGGGTAGTGTTCGCTGGAGCggta                                                                              >  2:166338/1‑135 (MQ=255)
                                                     tCCATGGCCCCACCCAGCGCCTGCAGCAAGGTTGATTTGCCGGCGCCGTTGCGGCCCAGCACGGCAATCCGCTCGCCCCCCGCGAGAATCAGCACCCCAAATTTCACGACAGGCGGGTAGTGATCGGTGTATCggga                                                                              >  4:355195/1‑135 (MQ=255)
                                                       cATGGCCCCACCCAGCGCCTGCAGCAAGGTTGATTTGCCGGCGCCGTTGCGGCCCAGCACGGCAATGCGCTCGCCCGGGGGGATATACAGACCGGCCATGTTGAGGACAGGGGGGTTGTGGTCGGGGGAGCGGaatt                                                                            >  5:162823/1‑135 (MQ=255)
                                                         tGGCCCCACCCAGCGCCTGCAGCAAGGTTGATTTGCCGGCGCCGTTGCGGCCCAGCACGGCAATGCGCTCGCCCGGCTGGATATCCAGCCGGCCAATGTTCAGCACTGGCGGGTAGTCTTCGCTGTAGCGGAAGTTg                                                                          <  8:208359/137‑1 (MQ=255)
                                                           gCCCCACCCAGCGCCTGCAGCAAGGTTGATTTGCCGGCGCCGTTGCGGCCCAGCACGGCAATGCGCTCGCCCGGCTGGATATCCAGCCGGCCAATGTTCAGCACTGGCGGGTAGTCtt                                                                                           <  3:312253/118‑1 (MQ=255)
                                                           gCCCCACCCAGCGCCTGCAGCAAGGTTGATTTGCCGGCGCCGTTGCGGCCCAGCACGGCAATGCGCTCGCCCGGCTGGATATCCAGCCGGCCAATGTTCAGCACTGGCGGGTAGTCtt                                                                                           >  4:312253/1‑118 (MQ=255)
                                                           gCCCCACCCAGCGCCTGCAGCAAGGTTGATTTGCCGGCGCCGTTGCGGCCCAGCACGGCAATGCGCTCGCCCGGCGGGATATCCAGCCGGCCCATGTTGATCACTGGCGGGGTGTCTTCGCTGGAGCGGAAGTTGGc                                                                        >  8:456283/1‑137 (MQ=255)
                                                            ccccACCCAGCGCCTGCAGCAAGGTTGATTTGCCGGCGCCGTTGCGGCCCAGCACGGCAATGCGCTCGCCCGGCGGGATTTCCAGCCGGCCAATGTTGAGCGCGGGCGGGGTGTGTTCGCGGTAGCGGGTGGTGGcc                                                                       >  4:91967/1‑137 (MQ=255)
                                                                         cggcagcaAGGTTGATTTGCCGGCGCCGTTGCGGCCCAGCACGGCAATGCGCTCGCCCGGCTGGATATCCAGCCGGCCAATGTTCAGCACTGGCGGGTAGTCTTCGCTGTAGCGGAAGTTGGCCTGGCGCAGGCGGt                                                          <  6:485841/135‑1 (MQ=255)
                                                                         cTGCAGCAAGGTTGATTTGCCGGCGCCGTTGCGGCCCAGCACGGCAATGCGCTCGCCCGGCTGGATATACATCCGGCCAATGTTCAGCACTGGCGGGTAGTCTTCGCTGTAGCGGCAGTTGGCCTGGCGCAGGCGGt                                                          >  6:332615/1‑137 (MQ=255)
                                                                             agcaAGGTTGATTTGCCGGCGCCGTTGCGGCCCAGCACGGCAATGCGCTCGCCCGGCGGGGTTTACAGCCGGCCCATGTTGAGCACGGGGGGGGTGTGTTCGCTGTTGCGGAAGTTGGGCGGGCGCCGGCGGTACta                                                      >  7:124850/1‑136 (MQ=255)
                                                                              gcaAGGTTGATTTGCCGGCGCCGTTGCGGCCCAGCACGGCAATGCGCTCGCCCGGCGGGATATCCAGCCCGCCCATGTTAAGCACTGGCGGGGTGTGTTAGGTGTAGCGGGAGTTGGCCTGGGGCAGGCGGTGCTCg                                                     >  4:270608/1‑137 (MQ=255)
                                                                                      gATTTGCCGGCGCCGATGCGGCCCAGCACGGCAATGCGCTCGCCCGGCTGGATATCCAGCCGGCCAATGTTCAGCACTGGCGGGTAGTCTTCGCTGTAGCGGAAGTTGGCCTGGCGCAGGCGGTACTCGCCGTGAAt                                             <  4:91463/137‑1 (MQ=255)
                                                                                          tGCCGGCGCCGTTGCGGCCCAGCACGGCAATGCGCTCGCGCGGCGGGCTATGCAGCCGGCCCAAGTTGAGCCCTGGCGGGTTGTGTTAGCGGTAGCGGAAGTTGGCCTGGGGCCGGCGGTAGGCGCCGTGGATGGcc                                         >  4:395171/1‑137 (MQ=255)
                                                                                          tGCCGGCGCCGTTGCGGCCCAGCACGGCAATGCGCTCGCCCGGCTGGATATCCAGCCGGCCAATGTTCAGCACTGGCGGGTAGTCTTCGCTGTAGCGGAAGTTGGCCTGGCGCAGGCGGTACTCGCCGTGAATGGcc                                         <  5:148928/137‑1 (MQ=255)
                                                                                          tGCCGGCGCCGTTGCGGCCCAGCACGGCAATGCGCTCGCCCGGCTGGATATCCAGCCGGCCAATGTTCAGCACTGGCGGGTAGTCTTCGCTGTAGCGGAAGTTGGCCTGGCGCAGGCGGTACTCGCCGTGAATGGcc                                         <  8:117057/137‑1 (MQ=255)
                                                                                                   cGTTGCGGCCCAGCACGGCAATGCGCTCGCCCGGCTGGATATCCAGCCGGCCAATGTTCAGCACTGGCGGGTAGTCTTCGCTGTAGCGGAAGTTGGCCTGGCGCAGGCGGTACTCGCCGTGAATGGCCGGCAGGTGc                                <  5:269422/137‑1 (MQ=255)
                                                                                                   cGTTGCGGCCCAGCACGGCAATGCGCTCGCCCGGCTGGATATCCAGCCGGCCAATGTTCAGCACTGGCGGGTAGTCTTCGCTGTAGCGGAAGTTGGCCTGGCGCAGGCGGTACTCGCCGTGAATGGCCGGCAGGTGc                                <  8:434788/137‑1 (MQ=255)
                                                                                                   cGTTGCGGCCCAGCACGGCAATGCGCTCGCCCGGCTGGATATCCAGCCGGCCAATGTTCAGCACTGGCGGGTAGTCTTCGCTGTAGCGGAAGTTGGCCTGGCGCAGGCGGTACTCGCCGTGAATGGCCGGCAGGTGc                                <  4:409239/137‑1 (MQ=255)
                                                                                                          gCCCAGCACGGCAATGCGCTCGCCCGGCTGTATATCCAGCCGGCCAATGTTCAGCACTGGCGGGTAGTCTTCGCTGTAGCGGAAGTTGGCCTGGCGCAGGCGGTACTCGCCGTGAATGGCCGGCAGGTGCACGCggg                         <  8:23092/137‑1 (MQ=255)
                                                                                                          gCCCAGCACGGCAATGCGCTCGCCCGGCTGGATATCCAGCCGGCCAATGTTCAGCACTGGCGGGTAGTCTTCGCTGTAGCGGAAGTTGGCCTGGCGCAGGCGGTACTCGCCGTGAATGGCCGGCAGGTGCACGCggg                         <  1:7432/137‑1 (MQ=255)
                                                                                                          gCCCAGCACGGCAATGCGCTCGCCCGGCTGGATATCCAGCCGGCCAATGTTCAGCACTGGCGGGTAGTCTTCGCTGTAGCGGAAGTTGGCCTGGCGCAGGCGGTACTCGCCGTGAATGGCCGGCAGGTGCACGCggg                         <  3:426118/137‑1 (MQ=255)
                                                                                                          gCCCAGCACGGCAATGCGCGCGCCCGGCTGGATATCCAGCCGGCCAATGTTCAGCACTGGCGGGTAGTCTTCGCTGTAGCGGAAGTTGGCCTGGCGCAGGCGGTACTCGCCGTGAATGGCCGGCAGGTGCACGCggg                         <  4:438800/137‑1 (MQ=255)
                                                                                                                cACGGCAATGCGCTCGCCCGGCTGGATATCCAGCCGGCCAATGTTCAGCACTGGCGGGTAGTCTTCGCTGTAGCGGAAGTTGGCCTGGCGCAGGCGGTACTCGCCGTGAATGGCCGGCAGGTGCACGCGGGCTTCGc                   <  2:399014/137‑1 (MQ=255)
                                                                                                                  cGGCAATGCGCTCGCCCGGCTGGATATCCAGCCGGCCAATGTTCAGCACTGGCGGGTAGTCTTCGCTGTAGCGGAAGTTGGCCTGGCGCAGGCGGTACTCGCCGTGAATGGCCGGCAGGTGCACGCGGGCTTCGCCt                 <  2:301766/137‑1 (MQ=255)
                                                                                                                  cGGCAATGCGCTCGCCCGGCTGGATATCCAGCCGGCCAATGTTCAGCACTGGCGGGTAGTCTTCGCTGTAGCGGAAGTTGGCCTGGCGCAGGCGGTACTCGCCGTGAATGGCCGGCAGGTGCACGCGGGCTTCGCCt                 <  8:406624/137‑1 (MQ=255)
                                                                                                                   ggCAATGCGCTCGCCCGGCTGGATATCCAGTCGGCCAATGTTCAGCACTGGCGGGTAGTCTTCGCTGTAGCGGAAGTTGGCCTGGCGCAGGCGGTACTCGCCGTGAATGGCCGGCAGGTGCACGCggg                         >  8:288760/1‑128 (MQ=255)
                                                                                                                   ggCAATGCGCTCGCCCGGCTGGATATCCAGTCGGCCAATGTTCAGCACTGGCGGGTAGTCTTCGCTGTAGCGGAAGTTGGCCTGGCGCAGGCGGTACTCGCCGTGAATGGCCGGCAGGTGCACGCggg                         <  7:288760/128‑1 (MQ=255)
                                                                                                                        tGCGCTCGCCCGGCTGGATATACAGCCGGCCAATGTTGAGCACTGGGGGGTTGTCTTAGGTGTTGCGGATGTTTGGCTGGGGCAGGCGGGACTCGCCGGGAATGGCCGGCCGGTGCACGCGGGCTTCGCCGTGgggg           >  8:307955/1‑137 (MQ=255)
                                                                                                                         gcgcTCGCCCGGCTGGATATCCAGCCGGCCAATGTTCAGCACTGGCGGGTAGTCTTCGCTGTAGCGGAAGTTGGCCTGGCGCAGGCGGTACTCGCCGTGAATGGCCGGCAGGTGCACGCGGGCTTCGCCTTCGGGGt          <  2:24243/137‑1 (MQ=255)
                                                                                                                         gcgcTCGCCCGGCTGGATATCCAGCCGGCCAATGTTCAGCACTGGCGGGTAGTCTTCGCTGTAGCGGAAGTTGGCCTGGCGCAGGCGGTACTCGCCGTGAATGGCCGGCAGGTGCACGCGGGCTTCGCCTTCGGGGt          <  7:307955/137‑1 (MQ=255)
                                                                                                                         gcgcTCGCCCGGCGGGATATCCAGCCGGCCAATGTTCAGCACTGGCGGGTAGTCTTCGCTGTAGCGGAAGTTGGCCTGGCGCAGGCGGTACTCGCCGTGAATGGCCGGCAGGTGCACGCGGGCTTCGCCTTCGGGGt          <  4:344815/137‑1 (MQ=255)
                                                                                                                                 cccgCTGGATATACAGCCGGCCAATGTTCAGCACTGGCGGGTAGTCATCGCTGTAGGGGTAGTTGGCCTGGCGCAGGCGGTACTCGCCGTGAATGGCCGGCAGGTGCACGCGGGCTTCGCCTTCGGGGTGATCGccc  >  6:442998/4‑137 (MQ=255)
                                                                                                                                      |                                                                                                                                    
GGTCGAGGTGGGCCATGGCGATGCCGTCCAGGCTGATCTCGCCCTGCACCAGATCCATGGCCCCACCCAGCGCCTGCAGCAAGGTTGATTTGCCGGCGCCGTTGCGGCCCAGCACGGCAATGCGCTCGCCCGGCTGGATATCCAGCCGGCCAATGTTCAGCACTGGCGGGTAGTCTTCGCTGTAGCGGAAGTTGGCCTGGCGCAGGCGGTACTCGCCGTGAATGGCCGGCAGGTGCACGCGGGCTTCGCCTTCGGGGTGATCGACC  >  ACB143/916562‑916827

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: