Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1432,094,8080AC44.0% 11.6 / 16.8 50L186R (CTG→CGG) CJ019_01995hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (22/6);  new base C (11/11);  total (33/17)
Fisher's exact test for biased strand distribution p-value = 4.18e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CCGGGGCCATGCGGTCCAGTACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGGCCGGGC‑TGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACAGCGGGCAAGAA  >  ACB143/2094680‑2094938
                                                                                                                                |                                                                                                                                   
ccGGGGCCATGCGGTCCAGTACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACATAAGCCTACGGCTGCTTGCGCAAATACTGCACCCCGCGCTCGAAGGCCAGGCGCGcc                                                                                                                             >  7:171009/1‑137 (MQ=255)
     gCCATGCGGTCCAGTACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGAGCAAATACTTCACGCCGCGCTCGAAGGCCAGGCGCGCCTGGcc                                                                                                                        >  6:348788/1‑137 (MQ=255)
       cctGCGTTCCAGACCCGGGCCATGGGCCGGGGCCAACAGTATCTAAAGATACGCCTGCTGCTCTTCATCCACCTTCGGCGCCGGCTGCTTGCCCAAATACGGCACGCCGCGCGCGACGGCCAGGCGCGCCTGGCCgg                                                                                                                      <  4:213044/135‑1 (MQ=255)
        aTGCGGTCCAGTACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCCGGCGCGCCTGGCCggg                                                                                                                     >  5:353632/1‑137 (MQ=255)
                 aGTACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCCGGCCCGCCTGGCCGGGC‑GGGCCgtt                                                                                                           >  4:399119/1‑137 (MQ=255)
                 aGTACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCACGCGCGCCGGGCCGGGG‑TGGCCgtt                                                                                                           >  8:262562/1‑137 (MQ=255)
                   tACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCCCGCGCGCCGGGCCGGGC‑GGGCGttttt                                                                                                         >  1:257005/1‑137 (MQ=255)
                         ggCATGGGCCCGGGCCAACAGTTTCTCAAGATCCGCCCGCTGCTCTTCATACCCCTTCGGCTCCGGCTGCTTGCGCAAATACTCCCCGCCGCGCTCGAAGGCCAGGCGCGCCTGGCCGGGC‑TGGCCTTTTTGCAggg                                                                                                   <  8:347108/137‑1 (MQ=255)
                           cATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCCGGCGCGCCGGGCCCGGCGGGGCGTTTTTGCggggg                                                                                                  >  7:116281/1‑136 (MQ=255)
                           cATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGGCCGGGC‑GGGGCGTTTTGCAGGGcc                                                                                                 >  7:388012/1‑137 (MQ=255)
                           cATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGATTGCGCAAAAACTGCACGCCGCGCTCGAAGGCCAGGCCCGCCGGGCCGGGC‑TGGCCGTTTTTCAGGGcg                                                                                                 >  7:488675/1‑136 (MQ=255)
                                gCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGACGGCCCCGCGCGCCTGGCCGGGC‑TGGCCGTTTTGCAGGGCCTGTTg                                                                                            >  6:149142/1‑137 (MQ=255)
                                   cGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGGCCGGGC‑TGGCCGTTTTGCAGGGCCAGTTGGcc                                                                                         >  2:88880/1‑137 (MQ=255)
                                        cAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCCCGCGCGCCGGGCCGGGC‑GGGGCGTTTTGCAGGGCCGGTTGGGCGAggg                                                                                    >  1:79904/1‑136 (MQ=255)
                                        cAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCAGCGGCGCCAGGCCGGGC‑TGGGCGTTTTTCAGGGGCGGGTGGGCGAggg                                                                                    >  1:452813/1‑136 (MQ=255)
                                                 ctaactaTACGAAAGCTGCCATACATACACCTTTGGTTCGCGCTGAGTGCCCAAATACTGCACTCCGCGATCGAAGGCAAGGCGCCCAGGGCCTGGC‑TGGCCTTTTTGCAGGGACTGTTGGCCGATGTTGTTgaaga                                                                           <  6:458322/131‑1 (MQ=255)
                                                   cAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCCCGCGCCGCTGCCCCCGC‑TGGCCCGTTTGCCGTGCCGGTTTTCCGAGCGTGGTgaagaaa                                                                         >  1:444623/1‑136 (MQ=255)
                                                   cAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCACGCGCGCCTGGCCGGGC‑TGGCCGTTTTGCAGGGGCTGTTGGCCGAGGGTGTTGATGAAc                                                                         >  4:423393/1‑137 (MQ=255)
                                                   cAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCACGCGCCCCTGGCCGGGC‑TGGGCTTTTTTAAGGGCCTGTTGGGAGAGGTTGTTttagaaa                                                                         >  4:448174/1‑136 (MQ=255)
                                                         aCGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCAGGCGCGCCAGGCCGGGC‑TGGCCTTTTTTCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCtata                                                                   >  5:393386/1‑137 (MQ=255)
                                                           ttCAGCTGCTCTTAATCCAGCTTCGGCGCGGGCTGGTTCCGAAAATACTGCCCGCCGCGCTCGAAGGCCCGGCGCGCCTGGCCGGGC‑TGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATg                                                                 <  5:391773/136‑1 (MQ=255)
                                                            tCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCCGGCGCGCCTGGCCGGGC‑TGGCCGTTTTGCAGGGCCGGTTGGCCGAGGTTGTTGAAGAACGCTATATgg                                                                >  2:214129/1‑136 (MQ=255)
                                                            gccggtgctCTTAAAACACCTTGGGCTCGGTGTGCTTGCGCAAATAGGGCTCGCCGCGCTCGAAGGCGCGGCGCGCGTGGCCGGGC‑TGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATgc                                                                <  2:301309/132‑1 (MQ=255)
                                                                 ggctCTTTATCCACCTACGGCTCGGGGTGCTTCTCCAATTAGTCCCCGGCGCGCTCGAAGCCCCGGCGCGCCGGGCCGGGC‑TGGCCTTTTTGGAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATgcagcag                                                           <  2:373071/136‑1 (MQ=255)
                                                                 ggctCTTCATACCCGGTCGCCGCGGGCTGCTTGCGCAAAACGCGCCCGCCGCGCGCGAAGGCCAGCCGCGCCGGGCCGGGC‑TGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATgcagcag                                                           <  6:298861/136‑1 (MQ=255)
                                                                        cATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCCCGCGCGCCAGGCCGGGC‑TGGCCGTTTTGCAGGGCCTGTTTGCCCAGGTTGTTGAAGAACTCTATATTCAGCAGCACCCgg                                                    >  5:281020/1‑137 (MQ=255)
                                                                           acacCTTCGGCTCCGGCTGCTTCCGAAAAAACTGCACGCCGCGCGCGACGGCCAGGCGCGCCTGGCCGGGC‑TGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGata                                                 <  8:457491/137‑1 (MQ=255)
                                                                                 tCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGGCCGGGC‑TGGCCGTTTTGCAGGGCCTGTTGGGCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCgg                                           >  8:185391/1‑137 (MQ=255)
                                                                                   ggCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGGCCGGGC‑TGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATTTGCAGGAGCACCAGGATATGGCGGAt                                         >  4:424202/1‑137 (MQ=255)
                                                                                   ggCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGGCCGGGC‑TGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCgggt                                         >  5:252855/1‑135 (MQ=255)
                                                                                   ggCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAACGCCACGCCCGCCAGGACGGGC‑TGGCCGTTTTGGAGGGCCAGTTGGCCGACGTTGTTTAAGAACACTATAAGAAGCAGCACCAGGATATGGCggtt                                         >  2:295011/1‑135 (MQ=255)
                                                                                     cgccGGCTGCTTGCCCAAATACTCCCCGCCGCGCGCGACGCCCCGGCGCGCCTGGCCGGGC‑TGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGAttt                                       <  2:122276/135‑1 (MQ=255)
                                                                                     cTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCCGGCGCGCCTGGCCGGGC‑TGGCCTTTTTGCAGGGCCTGTTGGCCGCGGTTGTTGTAGAACTCTTTATGCAGCAGCCCCAGGATATGGCGGAttt                                       >  7:390057/1‑137 (MQ=255)
                                                                                     cTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGGCCGGGC‑TGGCCTTTTTGCAGGGGCGGTTGGGCGAGGTTGTTGTAGAACTCTATATGCAGCAGCACCAGGATATGGCGGAttt                                       >  2:60539/1‑137 (MQ=255)
                                                                                     cTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGGCCGGGC‑TGGCCTTTTTGCAGGGCCTGTTGGCCGCGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGAttt                                       >  4:4546/1‑137 (MQ=255)
                                                                                     cTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGGCCCGGC‑TGGGCGTTTTGCAGGGGCTGGTTGCCGAGGTTGTTGAAGAACTCTATATTCAACAGCACCCGGATATGGCggctgt                                       >  8:281127/1‑133 (MQ=255)
                                                                                            tgctTGCGCAAATACTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGGCCGGGC‑TGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGAc                                >  6:72721/1‑137 (MQ=255)
                                                                                                tGCGCAAATACTGCACGCCGCGCGCGACGGCCCGGCGCGCCTGGCCGGGC‑TGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCaa                            <  8:388012/137‑1 (MQ=255)
                                                                                                ggcgaAAATACTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGGCCGGGC‑TGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCaa                            <  8:116281/136‑1 (MQ=255)
                                                                                                  ccaaaaTACGCCCCGCCGCGCTCGACGGCCCGGCGCGCCGGGCCGGGC‑TGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATg                          <  5:430001/134‑1 (MQ=255)
                                                                                                             cccgccgcGCGCGCCCGCCCGCCCCGCCGGGCCGGGC‑TGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGa               <  7:262562/135‑1 (MQ=255)
                                                                                                             cACGCCGCGCTCGAAGGCCCGGCGCGCCGGGCCGGGC‑TGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGa               <  2:272504/137‑1 (MQ=255)
                                                                                                               cgccgcGCTCGAAGGCCAGGCGCGCCTGGCCGGGC‑TGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACa             >  1:28782/1‑137 (MQ=255)
                                                                                                               cgccgcGCTCGAAGGCCAGGCGCGCCTGGCCGGGC‑TGGCCTTTTTGCAGGGCCTGGTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACa             >  4:297155/1‑137 (MQ=255)
                                                                                                                gccgcGCTCGAAGGCCCCGCGCGCCTGGCCGGGC‑TGGCCGTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTATATATGAAGCAGCCCCATGATTAGGAGGTTTTCCTTGCACCCATGCTTGGCCGAGACAg            >  2:222741/1‑137 (MQ=255)
                                                                                                                gccgcGCTCGAAGGCCAGGCGCGCCTGGCCGGGC‑TGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGCACTCTATATGCAGCCGCACCAGGATATGGCGGTTTTCCTTGACCCAATGCTTGGCCTCGACAg            >  2:218387/1‑137 (MQ=255)
                                                                                                                     gcTCGAAGGCCAGGCGCGCCTGGCCGGGC‑TGGGCTTTTTGCCGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATTCAGCCGCACCAGGATTTGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACAGCGGGc       >  5:464658/1‑137 (MQ=255)
                                                                                                                        cgccggCCCGGCGCGCCTGGCCGGGC‑TGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACAGCGGGCaag    <  5:149142/133‑1 (MQ=255)
                                                                                                                        cccaGGCCCGGCGCGCCTGGCCGGGC‑TGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACAGCGGGCaag    <  7:404301/134‑1 (MQ=255)
                                                                                                                          ccggCCCGGCGCGCCGGGCCGGGC‑TGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACAGCGGGCaagaa  <  4:169322/135‑1 (MQ=255)
                                                                                                                                |                                                                                                                                   
CCGGGGCCATGCGGTCCAGTACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGGCCGGGC‑TGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACAGCGGGCAAGAA  >  ACB143/2094680‑2094938

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑