Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1432,171,2710GC32.7% 202.9 / 41.4 129V150V (GTG→GTC) CJ019_02060IS1182 family transposase ISPpu16
Reads supporting (aligned to +/- strand):  ref base G (62/12);  new base C (0/36);  total (63/66)
Fisher's exact test for biased strand distribution p-value = 5.40e-19
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 130 total aligned reads displayed.

AAACCATCGCCGACTTTCGCAAGGACAACGGTAAAGCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACC‑TCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAA‑GCA‑GT‑GAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTGGCCATGGCGAAGG  >  ACB143/2171135‑2171404
                                                                                                                                            |                                                                                                                                     
aaaCCATCGACGACATTCGCAAGGACAACGCTAAAGCCATTCGTAGCGTCTGCCGTCAGTTAGTGATCCTCTGCCGTAACA‑TCAAACTTTTCTCTGAGTCGATAATTGCCATCTACGGCAGCAAGTTCAAA‑GCA‑GT‑g                                                                                                                                       >  6:440631/1‑137 (MQ=255)
 aaCCATCGCCGACTTTCGCAAGGACAACGGTAAAGCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACC‑TCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAA‑GCA‑GT‑Ga                                                                                                                                      >  5:92899/1‑137 (MQ=255)
         ccGACTTTCGCAAGGACAACGGTAAAGCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACC‑TCAACCTTTACTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAA‑GCA‑GT‑GAACAATcgc                                                                                                                              >  1:53727/1‑137 (MQ=255)
                tCGCAAGGACAACTGTAAAGCCTTTCTAAGCGTCTGCGGTCAGTTCGTGATCCTCTGCCGTAACC‑TCAACTTTTTGTCTGAGGCTATAATTGCCATCTAGGGCAGCAAGTTAAAA‑GCA‑GT‑GAACAACCGCGACCGTa                                                                                                                       <  8:264945/137‑1 (MQ=255)
                tCGCAAGGACAACGGTAAAGCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACC‑TCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAA‑GCA‑GT‑GAACAATCGCGACCGTa                                                                                                                       >  6:144478/1‑137 (MQ=255)
                     cggACAACTGAAAAGCCTTTCGTCGCGGCGGCCGTCAGTTCGTTATCCTCTGCCGTAACC‑ACAACCTTTTGTCTGAGTCTATCATTCCCATCGCCGGCAGAAAGTACAAA‑GCA‑GT‑CAACAATCGCGACCGTAACTTc                                                                                                                  <  4:353798/136‑1 (MQ=255)
                          aaCGTTAAAGCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACC‑TCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAA‑GCA‑GT‑GAACAATCGCGACCGTAACTTCACCCa                                                                                                             <  8:389513/137‑1 (MQ=255)
                          aaCGGTAACGCCATTCGTAGCGTCTGCCGTCAGTTCGTGAACCTCTGCCGAAACC‑TCAACCTTTTCTCTGAGTCGATCATTGCCAGCGACGGCAGAAAGTACAAA‑GCA‑GT‑CAACAATCGCGACCGTAACTTCACCCa                                                                                                             <  2:351886/137‑1 (MQ=255)
                            ggtaaaaGCAATTCGTAGCGTCTGCGTTCATTTGGTTAGCATCTGCCTAAACC‑TCAACCTTTTCTCTGAGCCGATCATTGCCATCGAGGGCAGCAATTTAAAA‑GCT‑GT‑AAAAAACCGCGACCGTAACTTCACCCAgg                                                                                                           <  6:278868/133‑1 (MQ=255)
                            cGGTAAACCAATCCGTCCCGGCGGCCGTCTGGTCGTGATCCTCTCCCGACACC‑ACAACCTTTTCGCTGCGCCGATAATTCCCTCCCACGTCAGAAAGTACAAC‑CCC‑GT‑AAAAAACCGCGACCGTAACTTCACCCAgg                                                                                                           <  4:264199/137‑1 (MQ=255)
                            cGGAAAAGCAATTCGTAGCGGCTGCCGTCTGTTCGTTATCCTCTGCCGTAACC‑TAAACCTTTTCTCTGAGTCTATCATTGCCATCGACGGAAGAAAGTTCAAA‑GCA‑GT‑CAACAAGCGCGACCGTAACTTCACCCAgg                                                                                                           <  6:170717/137‑1 (MQ=255)
                                 aaGCCATTCTTCGCGTCTGCCTTCAGTTGGTGACCCTCTGCCGAAACC‑AAAAATTTTTCTCTTTGTCTATAATTCCCATCGCCGGCACAAATTTCAAC‑GCA‑TT‑AAACAACCGCGACCGTAACTTCACCCAGGCCAAg                                                                                                      <  2:118316/137‑1 (MQ=255)
                                    ccATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACC‑TCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAA‑GCA‑GT‑GAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTg                                                                                                   >  3:442352/1‑137 (MQ=255)
                                     cATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACC‑TCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAA‑GCA‑GT‑GAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGa                                                                                                  >  5:81237/1‑137 (MQ=255)
                                     cATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACC‑TCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAA‑GCA‑GT‑GAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGa                                                                                                  >  3:253107/1‑137 (MQ=255)
                                     cATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACC‑TCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAA‑GCA‑GT‑GAACAATCGCGACCGTAACTTCACACAGGCCAAGGTGa                                                                                                  >  1:395068/1‑137 (MQ=255)
                                     cATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACC‑TCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAA‑GCA‑GT‑GAACAATCGCGACCGTAACTTAACCCAGGCCAAGGTgt                                                                                                  >  3:232/1‑136 (MQ=255)
                                       ttCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACC‑TCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAA‑GCA‑GT‑GAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAg                                                                                                >  2:87371/1‑137 (MQ=255)
                                       ttCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACC‑TCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAA‑GCA‑GT‑GAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAg                                                                                                >  3:435841/1‑137 (MQ=255)
                                         cGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACC‑TCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAA‑GCA‑GT‑GAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGc                                                                                              >  8:192469/1‑137 (MQ=255)
                                          gtggCGGCTGCAGTCATTTGGTTTTCCTCT‑CCGTAACCATCAACCTTTGGTGTGCGTGGTTTTTTGAGAGCGAGGGCACCATGTTCAAA‑GAT‑GT‑GAACAAGCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGcc                                                                                             <  4:74807/134‑1 (MQ=255)
                                          gTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACC‑TCCACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAA‑GCA‑GT‑GAACAATCGAGCCCGTAACTTCACCCAGGCCAAGGTGCAGGcc                                                                                             >  8:242472/1‑137 (MQ=255)
                                          gTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACC‑TCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAA‑GCA‑GT‑GAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGcc                                                                                             >  8:53165/1‑137 (MQ=255)
                                          gTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACC‑TCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAA‑GCA‑GT‑GAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGcc                                                                                             >  5:33333/1‑137 (MQ=255)
                                          gTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACC‑TCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAA‑GCA‑GT‑GAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGcc                                                                                             >  1:118316/1‑137 (MQ=255)
                                          gTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACC‑TCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAA‑GCA‑GT‑GAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGcc                                                                                             >  5:418520/1‑137 (MQ=255)
                                            cccggcTGCCGTCAGTGCGTGATCCTCTGCCGAAACC‑TCACCCTTTTCTCGGAGTCCATCATTCCCATCGACGGCAGCAAGTTCAAA‑GCA‑GT‑CAAAAAGCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCg                                                                                           <  6:84976/132‑1 (MQ=255)
                                            aGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACC‑TCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAA‑GCA‑GT‑GAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGcccc                                                                                           >  8:350463/1‑136 (MQ=255)
                                            aGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACC‑TCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTACAAC‑GCA‑GT‑CAACAAGCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCg                                                                                           <  1:312100/137‑1 (MQ=255)
                                            aGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACC‑TCAACATTTTCTCTGACTCGATCAATGCCATGCACGGCAGCAAGTGAAAA‑GCA‑GT‑GAACAATAGCGACCCTAACTACACACAGGCCAAGGTGAAGGccag                                                                                           >  1:367418/1‑135 (MQ=255)
                                               ggcggCCGTCTTTTCGTTATCCTGCCCCTTAACC‑TAAACATTTTCTGTGAGTCCATCATTGCCATCCCCGGCAGCAATTACAAA‑GCC‑GT‑CAACAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCAt                                                                                        <  4:100488/133‑1 (MQ=255)
                                               ggcTGCCGTCAGTTCGTGATCCTCTGCCTAAACC‑TCAACCTTTTCTCTGAGTCGATCATTGCCATCGCCGGCAGAAAGTTCAAA‑GCA‑GT‑CAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCAt                                                                                        <  8:49626/135‑1 (MQ=255)
                                               gTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACC‑TCAACCTTTTCTCTGCGACGATCATTCCCAGCGACGGCAGCAAGTTCAAA‑GCA‑GT‑CAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCAt                                                                                        <  7:367184/137‑1 (MQ=255)
                                                 cTGCCGTCAGTTCGTGATCCTCTGCCGTAACC‑TCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAA‑GCA‑GT‑GAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATgc                                                                                      >  3:387884/1‑137 (MQ=255)
                                                      ttctgTTCGTGACCCTCGGCCGTAACC‑TAAACTTTTTGTCTGAGTCGATCATGGCCATCGACGGCAGAAAGTACAAA‑GCA‑GT‑CAACAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCaa                                                                                 <  6:33333/133‑1 (MQ=255)
                                                          gTTCGTGATCCTCTGCCGTAACC‑TCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAA‑GCA‑GT‑GAACAATCGCGACCGTAACTTCACCC‑GGGCAAGGTTAAGGCCCGCATGCAGCCAATccg                                                                            >  3:457777/1‑135 (MQ=255)
                                                           ttggttaTCCTCTGCCGTAACT‑TCAACTTTTTGTCTGAGGCGATAATTGCCATCGACGGCACCAAGTTCAAA‑GCT‑GT‑CAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCga                                                                            <  7:350463/131‑1 (MQ=255)
                                                           ttCGTGATCCTCTGCCGAAACC‑TCAACCTTTTCTCTGAGTCGATATTTGCCATCTACGGCAGCAATTTCAAC‑GCA‑GT‑GAACAAGCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCga                                                                            <  8:311138/137‑1 (MQ=255)
                                                           ttCGTCATCCTCGGCCTAAACC‑TCAACCTTTTCTCTGAGTCTAGCATTGCCATCGACGGCAGCAAGTACAAC‑GCA‑GT‑TAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCga                                                                            <  6:207765/137‑1 (MQ=255)
                                                           gtCGTGATCCTCTGCCTTAACC‑TCAAACTTTTCTCTGAGTCTATCATTCCCATCCAGGGCACCAAGTTCAAA‑GCA‑GT‑CAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCga                                                                            <  8:159305/136‑1 (MQ=255)
                                                                     tctGCCGTAACC‑TCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAATTTCAAA‑GCA‑GT‑TAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCCTGCAGCAAATCGAGCAGAGCAtt                                                                  >  1:216973/1‑137 (MQ=255)
                                                                       tcccttAACC‑TCACCCTTTTCTCTGCGTCCATCATTGCCAGCGCCGGAAG‑AATTTACAA‑CCA‑CTCCAAAAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa                                                                <  5:27527/132‑1 (MQ=255)
                                                                       tcccGTAACC‑ACAACCTTTTCTCTGAGTCGATAATTGCCATCGACGGCAGAAAGTACAAC‑GCA‑GT‑CAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa                                                                <  4:100715/135‑1 (MQ=255)
                                                                       tcccGAAACC‑TAAACCTTTTCTCTGAGTCCATCATTGCCAGCGCCGGCAGAAATTTAAAA‑GCA‑GT‑CAACAAGCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa                                                                <  5:144478/135‑1 (MQ=255)
                                                                       tGCCGAAACC‑ACAACTTTTTCTCTGAGTCGAAAATTGCCATCGACGGCACAAAGTACAAA‑GCA‑TT‑CAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa                                                                <  7:440362/137‑1 (MQ=255)
                                                                       ggcctaaaCC‑TAAACATTTTCTGTGAGGCTATAATTCCCATCGACGGAAGAAATTTAAAA‑CCA‑GT‑CAACAAGCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa                                                                <  7:149877/131‑1 (MQ=255)
                                                                       ggCCGTAACC‑ACAACCTTTTCTCTGTGTCTATCATTCCCAGCGCCGGCAGCAAGTCCAAA‑GCA‑GT‑TAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa                                                                <  8:61042/136‑1 (MQ=255)
                                                                       ggCCGAACCC‑TCAACATTTTCTCTGAGTCCATAATTGCAATCGACGGCACCAATTTAAAA‑GCA‑GT‑AAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa                                                                <  5:90564/136‑1 (MQ=255)
                                                                       gccggaaaCC‑TAAACGTTTTGTGTTCGCCGATCATTCCCATGGACGGCAGCAAGTACAAC‑CCC‑GT‑TAACAAGCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa                                                                <  4:253107/131‑1 (MQ=255)
                                                                       gcccctAACC‑TCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAC‑GCA‑TA‑CAACAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa                                                                <  5:361727/132‑1 (MQ=255)
                                                                       gcccGTAACC‑ACAACCTTTTCTCTGAGTCGATCATGGCCATCTCCGGAAGCAAGTTCAAA‑GCC‑GT‑GAAAACTCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa                                                                <  8:291230/135‑1 (MQ=255)
                                                                       ccccctAACC‑TAAACATTTGCTCTCTGTCTATCATTCCCACCCACGGCA‑CAAAGTAAAA‑CCACGT‑CACCAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa                                                                <  1:189360/132‑1 (MQ=255)
                                                                        cccGTAACC‑CCAACCTTTTCTCTGAGTCTAGCATTCCCAGCGCCGGCACCAAGTGCAAA‑CCA‑GT‑CAACAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAg                                                               <  3:414804/136‑1 (MQ=255)
                                                                          cGTAACC‑TCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAA‑GCA‑GT‑GAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAgcc                                                             >  7:159305/1‑136 (MQ=255)
                                                                               cc‑TCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAA‑GCA‑GT‑GAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCAtt                                                                  >  5:198768/1‑127 (MQ=255)
                                                                               cc‑TCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAA‑GCA‑GT‑GAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCAtt                                                                  <  6:198768/127‑1 (MQ=255)
                                                                               cc‑TCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAA‑GCA‑GT‑GAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTAcc                                                        >  5:423054/1‑137 (MQ=255)
                                                                                ccacAACCTTTTCTCTGAGTCGATAATTCCCAGCGACGGAAGCAAGTACAAA‑GCA‑TT‑CAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTAcc                                                        <  8:118899/134‑1 (MQ=255)
                                                                                       cTTTTCTCTGTTTCTATCTTTCACCCCGACGGCAGCAATTAAAAA‑GCA‑TT‑TAAAAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGc                                                 <  3:415611/137‑1 (MQ=255)
                                                                                       cTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAA‑GCA‑GT‑GAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGc                                                 >  6:137598/1‑137 (MQ=255)
                                                                                       cTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAA‑GCA‑GT‑GAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGc                                                 >  7:247679/1‑137 (MQ=255)
                                                                                       cTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAA‑GCA‑GT‑GAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGc                                                 >  4:11402/1‑137 (MQ=255)
                                                                                       cTTTTCTCTGAGTCGATATTTGCCATCGACGGCAGAAAGTTCAAA‑GCA‑GT‑TAACAAACGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGc                                                 <  6:418520/137‑1 (MQ=255)
                                                                                          ttCTCTGTGTCGATCATTGCCATCTAGGGCAGCATGTTCAAA‑GCT‑GT‑TAAAAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGAt                                              <  5:103643/137‑1 (MQ=255)
                                                                                            ctctGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAA‑GCA‑GT‑GAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATgg                                            >  8:126262/1‑137 (MQ=255)
                                                                                             tctGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAA‑GCA‑GT‑GAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGa                                           >  7:332318/1‑137 (MQ=255)
                                                                                             tctGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAA‑GCA‑GT‑GAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGa                                           >  5:351973/1‑137 (MQ=255)
                                                                                             tatGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAA‑GCA‑GT‑GAACAATAGCGACAGTAACTTCACCCAGGCCAACCAGAATGCCCGAAAGAAGCAAATCTAGAACAGCATTCAGAGCTACATTACAGCGATGGa                                           >  4:220842/3‑137 (MQ=255)
                                                                                              gtGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAA‑GCA‑GT‑CAACAAACGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGAt                                          <  5:222224/136‑1 (MQ=255)
                                                                                                   tctaTCATTCCCATCGCCGGCAGCAATTTCAAC‑GCA‑GT‑GAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGc                                     <  4:442352/134‑1 (MQ=255)
                                                                                                   tCGATCATTGCCATCGACGGCAGCAAGTTCAAA‑GCA‑GT‑GAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGc                                     >  4:195732/1‑137 (MQ=255)
                                                                                                   tCGATCATTGCCATCGACGGCAGCAAGTTCAAA‑GCA‑GT‑GAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGc                                     >  3:314203/1‑137 (MQ=255)
                                                                                                   tCGATCATTGCCATCGACGGCAGCAAGTTCAAA‑GCA‑GT‑CAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGc                                     <  6:338923/137‑1 (MQ=255)
                                                                                                   gcGATCATTGCAATCGACGGCACCAAGTAAAAA‑CCA‑GT‑CAACAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGc                                     <  2:254820/136‑1 (MQ=255)
                                                                                                   ccGATCATTGCCATCGCGGGCACAAAGTTCAAA‑GCA‑GT‑GAACAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGc                                     <  4:446328/136‑1 (MQ=255)
                                                                                                    cGATCATTGCCATCGACGGCAGCAAGTTCAAA‑GCA‑GT‑GAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCa                                    >  6:185069/1‑137 (MQ=255)
                                                                                                    cGATCATTGCCATCGACGGCAGCAAGTTCAAA‑GCA‑GT‑GAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCa                                    >  2:77808/1‑137 (MQ=255)
                                                                                                    cGATCATTGCCATCGACGGCAGCAAGTTCAAA‑GCA‑GT‑GAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCa                                    >  7:56924/1‑137 (MQ=255)
                                                                                                         atgcccATCGCCGGCACCAAGTCCAAA‑GCC‑GT‑CAACAAGCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCg                               <  4:302087/133‑1 (MQ=255)
                                                                                                         aTTGCCATCGACGGCAGCAAGTTCAAC‑GCA‑GT‑GAACAAACGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGAAAATCGAGCAGAGCATTGAGCCCTACCTTACAGAGATGGATTCGGCAGACCg                               >  6:33894/1‑137 (MQ=255)
                                                                                                         aTTGCCATCGACGGCAGCAAGTTCAAA‑GCA‑GT‑GAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCg                               >  6:387836/1‑137 (MQ=255)
                                                                                                         aTTGCCATCGACGGCAGCAAGTTCAAA‑GCA‑CT‑GAACAAACGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCg                               >  8:185824/1‑137 (MQ=255)
                                                                                                         aTTCCCATCGCCGGCAGCAAGTTAAAC‑GCA‑GT‑CAACAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCg                               <  3:276037/137‑1 (MQ=255)
                                                                                                              cATCGAAGGCAGCAAGTTCAAA‑GCA‑GT‑GAAAAATCGCGAACGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGATCATTGAGCGCTACCTTAGAGCGATGGCTTCGGCAGACCGGGCaa                          >  1:411306/1‑137 (MQ=255)
                                                                                                              cATCCACGGCACCAA‑TTCAAACCCA‑GT‑CAACAAGCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCaa                          <  5:257082/137‑1 (MQ=255)
                                                                                                              cATAGACGGCAGCAAGTTCAAA‑GCA‑GT‑GAACAATCGCGACCGTAACTTCACCCAGGCCAACGTGAAGGCCCGCAAGCACCAAATCGAGCAGAGCATTGAGAGCTACCTTACATCGATGGATTCGGCAGACCGGGCaa                          >  8:182501/1‑137 (MQ=255)
                                                                                                                    gggCAGCAATTTCAAA‑GCA‑GT‑CAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCgg                    <  2:91249/136‑1 (MQ=255)
                                                                                                                    cGGCAGCAAGTTCAAA‑GCA‑GT‑GAACAATCGCGAAAGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGAGATTGATTCGGCAGACCGTGCAATGCCgg                    >  8:216549/1‑137 (MQ=255)
                                                                                                                        agcaAGTTCAAA‑GCA‑GT‑GAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCg                                                                             >  7:432572/1‑76 (MQ=255)
                                                                                                                        agcaAGTTCAAA‑GCA‑GT‑GAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCg                                                                             <  8:432572/76‑1 (MQ=255)
                                                                                                                        agcaAGTTCAAA‑GCA‑GT‑GAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGCGCAATGCCGGAGGt                >  8:155548/1‑137 (MQ=255)
                                                                                                                         gcaAGTTCAAT‑GCT‑GT‑CACCACTCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTg               <  8:395697/137‑1 (MQ=255)
                                                                                                                         ccaAGTTCAAC‑GCA‑GT‑GAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTg               <  8:402854/136‑1 (MQ=255)
                                                                                                                         caaaGCTCAAA‑GCA‑GA‑CAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTg               <  1:121628/135‑1 (MQ=255)
                                                                                                                          cacgTTCAAA‑GCA‑GT‑GAACAATCGCGACCGTAACTTCACCCCGGCCAAGGTGACGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGATACATTACAGCGATGCATTCGGCATACCGGGTACTGGCGGAGGTgg              >  2:461606/4‑137 (MQ=255)
                                                                                                                          caAGTTCAAA‑GCA‑GT‑GAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATAGAGCGCTACCTGACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTgg              >  2:254323/1‑137 (MQ=255)
                                                                                                                                acaacccA‑GT‑AAACAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTGGCCATg         <  1:87371/131‑1 (MQ=255)
                                                                                                                                 aaa‑CCA‑GT‑CAACAAGCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTGGCCATGGc       <  4:314203/137‑1 (MQ=255)
                                                                                                                                       a‑GT‑GAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCTCTACATTACAGCGATGGATTCGGCAGACCGCGCAATGCCGGAGGTGGCCATGGCGAAgg  >  2:326156/1‑137 (MQ=255)
                                                                                                                                       a‑GT‑GAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTGGCCATGGCGAAgg  >  2:118221/1‑137 (MQ=255)
                                                                                                                                            |                                                                                                                                     
AAACCATCGCCGACTTTCGCAAGGACAACGGTAAAGCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACC‑TCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAA‑GCA‑GT‑GAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTGGCCATGGCGAAGG  >  ACB143/2171135‑2171404

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑