Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1433,440,3710TG50.0% 33.2 / 10.7 40Y33D (TAT→GAT) CJ019_03266hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (9/10);  new base G (19/0);  total (30/10)
Fisher's exact test for biased strand distribution p-value = 3.91e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGAAGGCTGCAGCAGCAGCGCTCGATCAGCTGGCTGAAGAACTGGAGGCGCGGC  >  ACB143/3440239‑3440506
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cAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCGGGCAGCCGGGGTGTGGCAGGCCAACGCCGATaa                                                                                                                                     >  3:462832/1‑137 (MQ=255)
 aaGGCCCTCTACAAGCGATAGATAGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCCGGCCAACGCCGATAAg                                                                                                                                    >  7:71739/1‑137 (MQ=255)
        tctACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCGGGGAGCCGGGGTGTGGCAGGCCCACGCCGATAAGgcgccgg                                                                                                                             >  4:373014/1‑134 (MQ=255)
           aCAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCCCGCACGCGGCGCCCGCGCCGCCGCGGTGAGGGAGGCGCACCCCAATAATgcgccccgcg                                                                                                                          >  1:172847/1‑131 (MQ=255)
                  agcgCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCGGGCCGCCTGGGGGTGGCGGGCCACCGCCGATAAGGCGCAGCTCGCGGCTCa                                                                                                                   >  5:436210/1‑137 (MQ=255)
                  agcgCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCCCGCTGGCGGCCCCCGGGCAGCCAGGGTGTGGCAGGCCAACGCCAATAAGGCGCCGCGCCCTGATCa                                                                                                                   >  4:354601/1‑137 (MQ=255)
                  agcgCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCACCCCGGGCGGCGCCCCGGCCGCCTGGGTGTGGGGCGGCAACGCCTATACGGCGCCGCTCGCTGATcg                                                                                                                   >  1:160009/1‑136 (MQ=255)
                  agcgCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCCCCCGCGCGGGCGGCGCCCCGGCAGCCGGGGTGTGGCAGGCCCACCCCGATAAGGCGCACCTCGCTGATCa                                                                                                                   >  8:33577/1‑137 (MQ=255)
                     gcTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCCGGCAGCCTGGGTGTGGCAGGCCCACGCCTATAACGCGCACCTCGCTGCTCCggg                                                                                                                >  6:217913/1‑136 (MQ=255)
                     gcTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCGGGCAGCCGGGGTGTGGCAGGCCACCCCCGATAAGGCGCCGCACGCGGCTAAGGc                                                                                                                >  6:486029/1‑137 (MQ=255)
                     gcTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCCGGCAGCCTGGGTGTGGGAGGCCCACGCCGATAATGCGCAGCTCGCTGATCAGGc                                                                                                                >  4:130472/1‑137 (MQ=255)
                       tCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCCCTGGCCGCGCCCGGGCAGCCTGGGTGTGGCAGGCCCACCCCGAATAAGCGCAGCTCGCCGATGAGGccc                                                                                                              >  4:400882/1‑136 (MQ=255)
                       tCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCGGGCGGCGCCCCGGCCGCCGGGGTGTGGCAGGCCAACCCCGATAAGGCGCAGCTCGCTGGTCAGGccc                                                                                                              >  6:389127/1‑136 (MQ=255)
                                         cgcTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCCGCCTGGGGGTGGGAGGCCACCGCCGATAAGGCGCAGCGCGCTGGTCAGGCCGCTGGCTTCGGCAAGCAGt                                                                                            >  3:453530/1‑137 (MQ=255)
                                          gcTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCGGGCCGCCTGGGGGTGGCCGGCCCACCCCGATAAGGCGCAGCTCGCTGATAAGGGCGCGGAATTCGGGAAGCAGtt                                                                                           >  7:19690/1‑137 (MQ=255)
                                          gcTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACCCCGATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGtt                                                                                           >  8:223772/1‑137 (MQ=255)
                                          gcTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCACCGCTGGCGGCGCCCCGGCCGCCCGGGGGTGGCAGGCCAACCCCGATAACGCGCAGCTCGCTGATCAGGCCGCAGCCATCCGCAAGCACtt                                                                                           >  7:368520/1‑137 (MQ=255)
                                             tGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCCGGGCCACGCCGATAAGGCGCAGCGCGCTGCTCAGGCCGGTGTCTTCGGGAAGCAGtttgg                                                                                        >  2:13904/1‑134 (MQ=255)
                                               ggCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCCCCCCCGCGGGCGGCGCCCGCGCCGCCGGGGGGTGGCAGGCCACACCCGATAAAGCCCCGCGCGCTGATCAGGCCGCTGACGTCGGCCAGCAGTTGGCgg                                                                                      >  4:145111/1‑137 (MQ=255)
                                                      cGCCTGGTCGCCGTGGCGCTGCTGCTGGGCGCCCGCGCGGGCGGCGCCCGGGCCGCCGGGGTGGGGGAGGCCCACCCCGATAAGGCGCCGCTCGCGGCTCAGGCCGATGACTTCGGCCCGCACTTGGGGGCGCAggg                                                                               >  8:377880/1‑136 (MQ=255)
                                                      cGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCGGGCCGCCGGGGTGTGGCAGGCCCACGCCGATAAGGCGCAGCTCGCGGATCAGGCCGATGACGTCGGCAAGCAGTTGGCGGGGAAggg                                                                               >  8:331881/1‑136 (MQ=255)
                                                       gCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCCGGCCGCCGGGGGGTGGCAGGCCCACGCCGATAAGGCGCAGCGCCGGGGTCAGGCCGATGACTTCGGCAAGCAGTTGGCGGCGAAGGAt                                                                              >  4:392573/1‑137 (MQ=255)
                                                           gggcggcGTGGCGGTGCGGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATcgcg                                                                          <  7:72084/131‑1 (MQ=255)
                                                                            ggggggCCGCCGGGGGGGGGGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCgg                                                         <  3:56857/133‑1 (MQ=255)
                                                                            ggcgggCCGCCCGCGCGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCgg                                                         <  8:71739/133‑1 (MQ=255)
                                                                                        gcgcGGGCGGCGCCCGGGCCGCCGGGGGGTGGCAGGCCCACGCCGATAAGGCGCCGCTCGCTGATCAGGCCGATGACTTCGGCAAGCACTTGGGGGCGAAGGCTCGCGCGCACGGTCTGGCGCGGGAGAAGGCTgcc                                             >  8:380749/1‑136 (MQ=255)
                                                                                            gggcggcGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGAAGGCTgcagcag                                         <  5:406415/136‑1 (MQ=255)
                                                                                              gcggcgCCCGGGCAGGCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGAAGGCTgcagcagca                                       >  3:335781/1‑137 (MQ=255)
                                                                                              gcggcgCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGAAGGCTgcagcagca                                       <  4:200714/137‑1 (MQ=255)
                                                                                                           tgCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGAGCGCGCGCACGGTCTGGAGCGGGAGAAGGCTGCAGCAGCAGCGCTCGATCAgc                          <  2:172847/136‑1 (MQ=255)
                                                                                                                      ggCATGCCAACGACTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGAAGGCTGCAGCAGCAGCGCTCGATCAGCTGGCTgaagaa               <  4:302658/137‑1 (MQ=255)
                                                                                                                          ggCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGaa                                                    <  7:140887/96‑1 (MQ=255)
                                                                                                                          ggCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGaa                                                    >  8:140887/1‑96 (MQ=255)
                                                                                                                          ggCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGAAGGCTGCAGCAGCAGCGCTCGATCAGCTGGCTGAAGAACTgg           >  3:278750/1‑137 (MQ=255)
                                                                                                                          ggCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGAAGGCTGCAGCAGCAGCGCTCGATCAGCTGGCTGAAGAACTgg           >  4:107052/1‑137 (MQ=255)
                                                                                                                          ggCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGAAGGCTGCAGCAGCAGCGCTCGATCAGCTGGCTGAAGAACTgg           >  4:137350/1‑137 (MQ=255)
                                                                                                                          ggCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGAAGGCTGCAGCAGCAGAGCTCGATCAGCTGGCTGAAGAACTgg           >  3:283585/1‑137 (MQ=255)
                                                                                                                                cGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGAAGGCTGCAGCAGCAGCGCTCGATCAGCTGGCTGAAGAACTGGAggcgc     >  6:253034/1‑137 (MQ=255)
                                                                                                                                 gCCTATAAGGAGCAGCTCGCTGATCAGGCCGATGACTTCGTCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGAAGGCTGCAGCAGCAGCGCTCGATCTGCTGGCTGAAGAACTGGAggcgcg    <  1:246261/137‑1 (MQ=255)
                                                                                                                                   cTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGAAGGCTGCAGCAGCAGCGCTCGATCAGCTGGCTGAAGAACTGGAggcgcggc  <  1:404326/137‑1 (MQ=255)
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CAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGAAGGCTGCAGCAGCAGCGCTCGATCAGCTGGCTGAAGAACTGGAGGCGCGGC  >  ACB143/3440239‑3440506

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑