Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | ACB143 | 6,003,100 | 0 | A | C | 38.1% | 89.2 / 17.8 | 63 | T257P (ACC→CCC) | CJ019_05617 | putative protein |
Reads supporting (aligned to +/- strand): ref base A (6/33); new base C (24/0); total (30/33) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 3.79e-12 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Rejected as consensus: Frequency below/above cutoff threshold. |
TCGCCGATGTGGTGGCCTTCAAGGGCCCGCACATCAACCACCTGACCCCGCGTACCCTGGATATCGATGCAATCCAGCTCGGCATGCCTGCCAAGGGCATCCCGCCGAAGGCCGTGGTTGAAGGCCCGCCCACCCGCCGCCACCCGATCCTGCTGCGCCAGACCAGCTTCAAGGCCCTGCAGGAAACGGTCGCCTTCCGCGACCAACAAGGCCGCGAAGGCAGCCACACCGCACGCTTTGGCGAAATCGAGCAACGCGGCGCG > ACB143/6002969‑6003231 | tCGCCGATGTGGTGGCCTTCAAGGGCCCGCACATCAACCACCTGACCCCGCGTACCCTGGATATCGATGCAATCCAGCTCGGCATGCCTGCCAAGGGCATCCCGCCGAAGGCCGTGGTTGAAGGCCCGCCCACccgc < 2:415526/137‑1 (MQ=255) tCGCCGATGTGGTGGCCTTCAAGGGCCCGCACATCAACCACCTGACCCCGCGTACCCTGGATATCGATGCAATCCAGCTCGGCATGCCTGCCAAGGGCATCCCGCCGAAGGCCGTGGTTGAAGGCCCGCCCACccgc < 2:408671/137‑1 (MQ=255) aTGTGGTGGCCTTCAAGGGCCCGCACATCAACCACCTGACCCCGCGTACCCTGGATATCGATGCAATCAAGCTCGGCATGCCTGCCAAGGGCATCCCGCCGAAGGCCGTGGTTGAAGGCCCGCCCACCCGCCGCCAc > 6:171614/1‑137 (MQ=255) gtggtgGCCTTCAAGGGCCCGCACATCAACCACCTGACCCCGCGTACCCTGGATATCGATGCAATCCAGCTCGGCATGCCTGCCAAGGGCATCCCGCCGAAGGCCGTGGTTGAAGGCCCGCCCACCCGCCGCCAccc < 1:225128/137‑1 (MQ=255) ggCCTTCAAGGGCCCGCACATCAACCACCTGACCCCGCGTACCCTGGATATCGATGCAATCCAGCTCGGCATGCCTGCCAAGGGCATCCCGCCGAAGGCCGTGGTTGAAGGCCCGCCCACCCGCCGCCACCCGATcc < 5:15395/137‑1 (MQ=255) ccTTCAAGGGCCCGCACATCAACCACCTGACCCCGCGTACCCTGGATATCGATGCAATCCAGCTCGGCATGCCTGCCAAGGGCATCCCGCCGAAGGCCGTGGTTGAAGGCCCGCCCCCCCCCCGCCCCCCCCTCctg > 4:360076/1‑137 (MQ=255) aaGGGCCCGCACATCAACCACCTGACCCCGCGTACCCTGGATATCGATGCAATCCAGCTCGGCATGCCTGCCAAGGGCATCCCGCCGAAGGCCGTGGTTGAAGGCCCGCCCCCCCGCCGCCCCCCCATCCTGCTgcg > 7:132034/1‑137 (MQ=255) gggCCCGCACATCAACCCCCTGACCCCGCGTACCCTGGATATCGATGCAATCCAGCTCGGCATGCCTGCCAAGGGCATCCCGCCGAAGGCCGTGGTTGAAGGCCCGCCCCCCCGCCGCCCCCCCAACctgctggggc > 1:13952/1‑133 (MQ=255) gCCCGCACATCAACCACCTGACCCCGCGTACCCTGGATATCGATGCAATCCAGCTCGGCATGCCTGCCAAGGGCATCCCGCCGCAGGCCGTGGTTGAAGGCCCGCCCACCCGCCGCCACCCGATCCTGCTGCGCCAg < 1:366829/137‑1 (MQ=255) gCCCGCACATCAACCACCTGACCCCGCGTACCCTGGATATCGATGCAATCCAGCTCGGCATGCCTGCCAAGGGCATCCCGCCGAAGGCCGTGGTTGAAGGCCCGCCCACCCGCCGCCACCCGATCCTGCTGCGCCAg < 6:340158/137‑1 (MQ=255) gCCCGCACATCAACCACCTGACCCCGCGTACCCTGGATATCGATGCAATCCAGCTCGGCATGCCTGCCAAGGGCATCCCGCCGAAGGCCGTGGTTGAAGGCCCGCCCACCCGCCGCCACCCGATCCTGCTGCGCCAg < 1:358890/137‑1 (MQ=255) gCCCGCACATCAACCACCTGACCCCGCGTACCCTGGATATCGATGCAATCCAGCTCGGCATGCCTGCCAAGGGCATCCCGCCGAAGGCCGTGGTTGAAGGCCCGCCCACCCGCCGCCACCCGATCCTGCTGCGCCAg < 4:281117/137‑1 (MQ=255) gCCCGCACATCAACCACCTGACCCCGCGTACCCTGGATATCGATGCAATCCAGCTCGGCATGCCTGCCAAGGGCATCCCGCCGAAGGCCGTGGTTGAAGGCCCGCCCACCCGCCGCCACCCGATCCTGCTGCGCCAg < 3:157576/137‑1 (MQ=255) gCCCGCACATCAACCACCTGACCCCGCGTACCCTGGATATCGATGCAATCCAGCTCGGCATGCCTGCCAAGGGCATCCCGCCGAAGGCCGTGGTTGAAGGCCCGCCCACCCGCCGCCACCCGATCCTGCTGCGCCAg < 5:14846/137‑1 (MQ=255) gCCCGCACATCAACCACCTGACCCCGCGTACCCTGGATATCGATGCAATCCAGCTCGGCATGCCTGCCAAGGGCATCCCGCCGAAGGCCGTGGTTGAAGGCCCGCCCACCCGCCGCCACCCGATCCTGCTGCGCCAg < 1:289878/137‑1 (MQ=255) gCCCGCACATCAACCACCTGACCCCGCGTACCCTGGATATCGATGCAATCCAGCTCGGCATGCCTGCCAAGGGCATCCCGCCGAAGGCCGTGGTTGAAGGCCCGCCCACCCGCCGCCACCCGATCCTGCTGCGCCAg < 7:458162/137‑1 (MQ=255) acaTCAACCACCTGACCCCGCGTACCCTGGATATCGATGCAATCCAGCTCGGCATGCCTGCCAAGGGCATCCCGCCGAAGGCCGTGGTTGAAGGCCCGCCCCCCCGCCGCCACCCGAACCTGCTGCGCCAGACCAGc > 6:209837/1‑137 (MQ=255) acaTCAACCACCTGACCCCGCGTACCCTGGATATCGATGCAATCCAGCTCGGCATGCCTGCCAAGGGCATCCCGCCGAAGGCCGTGGTTGAAGGCCCGCCCCCCCGCCCCCCCCCCCAACTGCGGCGCCAGAccccc > 7:470315/1‑134 (MQ=255) acaTCAACCACCTGACCCCGCGTACCCTGGATATCGATGCAATCCAGCTCGGCATGCCTGCCAAGGGCATCCCGCCGAAGGCCGTGGTTGAAGGCCCGCCCCCCCCCCCCCCCCCGATCCTGCTGCGCCAGAGCacc > 1:167837/1‑135 (MQ=255) acaTCAACCACCTGACCCCGCGTACCCTGGATATCGATGCAATCCAGCTCGGCATGCCTGCCAAGGGCATCCCGCCGAAGGCCGTGGTTGAAGGCCCCGCCCCCCCCCCCCACCCCCTACAGCTGCGCCAGCCCacc > 7:463516/1‑135 (MQ=255) caTCAACCACCTGACCCCGCGTACCCTGGATATCGATGCAATCCAGCTCGGCATGCCTGCCAAGGGCATCCCGCCGAAGGCCGTGGTTGAAGGCCCGCCCACCCGCCGCCACCCGATCCTGCTGCGCCAGACCAGCt < 6:112969/137‑1 (MQ=255) cAACCTCCTGACCCCGCGTTCCCTGGATATCGATGCAATCCAGCTCGGCATGCCTGCCAAGGGCATCCCGCCGAAGGCCGTGGTTGAAGGCCCGCCCACCCGCCGCCACCCGATCCTGCTGCGCGAGACCAGCTTCa < 2:285899/137‑1 (MQ=255) accaccTGACCCCGCGTACCCTGTATATCGATGCAATCCAGCTCGGCATGCCTGCCAAGGGCATCCCGCCGAAGGCCGTGGTTGAAGGCCCGCCCACCCGCCGCCACCCGATCCTGCTGCGCCAGACCAGCTTCAAg < 1:290894/137‑1 (MQ=255) accaccTGACCCCGCGTACCCTGGATATCGATGCAATCCAGCTCGGCATGCCTGCCAAGGGCATCCCGCCGAAGGCCGTGGTTGAAGGCCCGCCCACCCGCCGCCACCCGATCCTGCTGCGCCAGACCAGCTTCAAg < 1:151404/137‑1 (MQ=255) accaccTGACCCCGCGTACCCTGGATATCGATGCAATCCAGCTCGGCATGCCTGCCAAGGGCATCCCGCCGAAGGCCGTGGTTGAAGGCCCGCCCACCCGCCGCCACCCGATCCTGCTGCGCCAGACCAGCTTCAAg < 3:294438/137‑1 (MQ=255) ccaccTGACCCCGCGTACCCTGGATATCGATGCAATCCAGCTCGGCATGCCTGCCAAGGGCATCCCGCCGAAGGCCGTGGTTGAAGGCCCCCCCCCCCCCCCCCCCCCCCTCCCGCAGCGGCCGACCCCCGTCAAgg > 1:186097/1‑137 (MQ=255) gACCCCGCGTACCCTGGATATCGATGCAATCCAGCTCGGCATGCCTGCCAAGGGCATCCCGCCGAAGGCCGTGGTTGAAGGCCCGCCCCCCCCCCGCCCCCCCAACCAGCTGCGCCAGACCAGCGTCAAGGCCCGGc > 3:78445/1‑137 (MQ=255) ccccGCGTACCCTGGATATCGATGCAATCCAGCTCGGCATGCCTGCCAAGGGCATCCCGCCGAAGGCCGTGGTTGAAGGCCCCCCCACCCCCCCCCCCCCCCAACAGCTGCGCCACACCCGCGTGAACGCCCCGCac > 3:438136/1‑136 (MQ=255) ccGCGTACCCTGGATATCGATGCAATCCAGCTCGGCATGCCTGCCAAGGGCATCCCGCCGAAGGCCGTGGTTGAAGGCCCGCCCCCCCCCCCCCCCCCCCTCCTGCTGCGCCCGACCAGCGTCCAGGCCCGGCAggg > 3:312295/1‑136 (MQ=255) ccGCGTACCCTGGATATCGATGCAATCCAGCTCGGCATGCCTGCCAAGGGCATCCCGCCGAAGGCCGTGGTTGAAGGCCCGCCCCCCCCCCCCCCCCCCCACCTGCGGCGCCAGACCAGCGTAAAGGCCCGGCACGa > 1:312978/1‑137 (MQ=255) tACCCTGGATATCGATGCAATCCAGCTCGGCATGCCTGCCAAGGGCATCCCGCCGAAGGCCGTGGTTGAAGGCCCGCCCCACCGCCCCCCCCCCATACCGCGGCGCCACCCCACCTTAAAGGGCCGGCAGGAAAAgg > 2:65592/1‑137 (MQ=255) aCCCTGGATATCGATGCAATCCAGCTCGGCATGCCTGCCAAGGGCATCCCGCCGAAGGCCGTGGTTGAAGGCCCCCCCCCCCCCCCCCCCCCCAAACAGCTGCGCCAGCCCCCCTTAAAAGCCCAGCAGGGAACCGt > 3:222911/1‑137 (MQ=255) tGGATATCGATGCAATCCAGCTCGGCATGCCTGCCAAGGGCATCCCGCCGAAGGCCGTGGTTGAAGGCCCGCCCCCCCCCCCCCCCCCCAAACGTCTGCCCCAGCCCCGCGTCACGGCCCGGCAGGAAAAGGTAGcc > 8:28217/1‑137 (MQ=255) tGGATATCGATGCAATCCAGCTCGGCATGCCTGCCAAGGGCATCCCGCCGAAGGCCGTGGTTGAAGGCCCGCCCACCCGTCGCCACCCGATCCTGCTGCGCCAGACCAGCTTCAAGGcc > 1:341563/1‑119 (MQ=255) tGGATATCGATGCAATCCAGCTCGGCATGCCTGCCAAGGGCATCCCGCCGAAGGCCGTGGTTGAAGGCCCGCCCACCCGTCGCCACCCGATCCTGCTGCGCCAGACCAGCTTCAAGGcc < 2:341563/119‑1 (MQ=255) gATATCGATGCAATCCAGCTCGGCATGCCTGCCAAGGGCATCCCGCCGAAGGCCGTGGTTGAAGGCCCGCCCACCCGCCGCCACCCGATCCTGCTGCGCCAGACCAGCTTCAAGGCCCTGCAGGAAACGGTCGCCtt < 5:207080/137‑1 (MQ=255) gATATCGATGCAATCCAGCTCGGCATGCCTGCCAAGGGCATCCCGCCGAAGGCCGTGGTTGAAGGCCCCCCCCCCCCCCCCCACCCCCTCCTGACGCCGCACCCCCGCTGCAACGCCCAGCCGGAAACGGTCGccgt > 3:14814/1‑135 (MQ=255) tatCGATGCAATCCAGCTCGGCATGCCTGCCAAGGGCATCCCGCCGAAGGCCGTGGTTGAAGGCCCGCCCACCCGCCGCCACCCGATCCTGCTGCGCCAGACCAGCTTCAAGGCCCTGCAGGAAACGGTCGCCTTcc < 3:78693/137‑1 (MQ=255) tatCGATGCAATCCAGCTCGGCATGCCTGCCAAGGGCATCCCGCCGAAGGCCGTGGTTGAAGGCCCGCCCACCCGCCGCCACCCGATCCTGCTGCGCCAGACCAGCTTCAAGGCCCTGCAGGAAACGGTCGCCTTcc < 5:143393/137‑1 (MQ=255) atCGATGCAATCCAGCTCGGCATGCCTGCCAAGGGCATCCCGCCGAAGGCCGTGGTTGAAGGCCCGCCCACCCGCCGCCACCCGATCCTGCTGCGCCAGACCAGCTTCAAGGCCCTGCAGGAAAAGGTAGCCTTccc > 1:291262/1‑136 (MQ=255) atCGATGCAATCCAGCTCGGCATGCCTGCCAAGGGCATCCCGCCGAAGGCCGTGGTTGAAGGCCCGCCCACCCCCCGCCCCCCGCAACAGCTGCGCCCGCCCCCCGTGAATGCCCGGCCGGTAACCGTCGccatacc > 2:439762/1‑132 (MQ=255) gCAATCCTGCTCGGCTGGCCTGCCAAGGGCAGCCCGCCGAAGGCGGTGGTGGCCGGCCGGCCCACCCGCCGCCACCCGATCGTGCTGCGCGAGACCAGCTTCAAGGCCCTGCCGTAAACGGTCGCCTTCCGCGACca < 4:221641/137‑1 (MQ=255) gCAATCCAGCTCGGCATGCCTGCCAAGGGCATCCCGCCGAAGGCCGTGGTTGAAGGCCCGCCCCCCCCCCGCCCCCCCATCCAGGTGGGCCAGCCCAGCTTCAAGGCCCGTCAGGAAACGGTACCCTTACGCCAccc > 7:489847/1‑136 (MQ=255) gCAATCCAGCTCGGCATGCCTGCCAAGGGCATCCCGCCGAAGGCCGTGGTTGAAGGCCCGCCCACCCGCCGCCACCCGATCCTGCTGCGCCAGACCAGCTTCAAGGCCCTGCAGGAAACGGTCGCCTTCCGCGACca < 2:109216/137‑1 (MQ=255) gCAATCCAGCTCGGCATGCCTGCCAAGGGCATCCCGCCGAAGGCCGTGGTTGAAGGCCCGCCCACCCCCCCCCACCCGATACAGGTGCGCCACACCAGCATAAACGCCCGGCAGGAAACCGTAGCCATCCCCGcccc > 6:207080/1‑136 (MQ=255) aaTCCAGCTCGGCATGCCTGCCAAGGGCATCCCGCCGAAGGCCGTGGTTGAAGGCCCGCCCACCCGCCGCCCCCCGATCCTGCTGCGCCAGACCAGCTTCAAGGCCCTGCAGGAAACGGTCGCCTTCCGCGACcaac < 4:353609/137‑1 (MQ=255) tCCAGCTCGGCATGCCTGCCAAGGGCATCCCGCCGAAGGCCGTGGTTGAAGGCCCGCCCCCCCCCCCCCCCCCCCTACAGGTGCGCCCCCCCACCTTCAAAGCCCCGCAGGAAACCGTAGCCGTACCCGCCccacaa > 6:53737/1‑137 (MQ=255) cAGCTCGGCATGCCTGCCAAGGGCATCCCGCCGAAGGCCGTGGTTGAAGGCCCGCCCACCCGCCGCCACCCGATCCTGCTGCGCCAGACCAGCTTCAAGGCCCTGCAGGAAACGGTCGCCTTCCGCGACCAACAAgg < 1:65592/137‑1 (MQ=255) gCTCGGCATGCCTGCCAAGGGCATCCCGCCGAAGGCCGTGGTTGAAGGCCCGCCCACCCGCCGCCACCCGATCCTGCTGCGCCAGACCAGCTTCAAGGCCCTGCAGGAAACGGTCGCCTTCCGCGACCAACAAGGcc < 4:189623/137‑1 (MQ=255) tCGGCATGCCTGCCAAGGGCATCCCGCCGAAGGCCGTGGTTGAAGGCCCGCCCACCCGCCGCCACCCGATCCTGCTGCGCCAGACCAGCTTCAAGGCCCTGCAGGAAACGGTCGCCTTCCGCGACCAACAAGGCcgc < 7:122777/137‑1 (MQ=255) tCGGCATGCCTGCCAAGGGCATCCCGCCGAAGGCCGTGGTTGAAGGCCCGCCCACCCGCCGCCACCCGATCCTGCTGCGCCAGACCAGCTTCAAGGCCCTGCAGGAAACGGTCGCCTTCCGCGACCAACAAGGCcgc < 3:166363/137‑1 (MQ=255) ggCATGCCTGCCAAGGGCATCCCGCCGAAGGCCGTGGTTGAAGGCCCGCCCCCCCGCCGCCCCCCGATCCTGCTGCGCCAGACCAGCTTAAAGGCCCGGCAGGAAACGGTCGCCTTCCGCGCCCAACAAGGCCGCGa > 6:469347/1‑137 (MQ=255) ggCATGCCTGCCAAGGGCATCCCGCCGAAGGCCGTGGTTGAAGGCCCCCCCCCCCGCCGCCCCCCGAACCAGCGGCGCCAGACCCGCTTCAAGGCCCGGGAGGCAACCGTCGCCGTGCGCGTCCAACAACGGcgcgg > 5:82069/1‑136 (MQ=255) gcctgccAAGGGCATCCCGCCGAAGGCCGTGGTTGAAGGCCCGCCCCCCCCCCGCCCCCCGCTACTGGGGCGGCAGCCCCGGGTGAAGGGCCTGCAGGAAAACGTCGCCGGCCGCGACCCACCACGCcgcggcggcc > 8:353251/1‑131 (MQ=255) gcctgccAAGGGCATCCCGCCGAAGGCCGTGGTTGAAGGCCCCCCCCCCCGCCGCCCCCCCCACCAGCTGCGGCCGCCCCGCGTGCAGGCCCGGCCGGGAACCGTCGCCGTACGCGGCCCACCACGCcgcgcacggc > 8:464378/1‑131 (MQ=255) cctgccAAGGGCATCGCGCCGAAGGCGGTGGTTGAAGGCCCGCCCACCCGCCGCCACCCGTTCCTGCTGCGCCAGACCAGCTTCAAGGCCCTGCTGGAAACGGTCGCCTTCCGCGACCAACAAGGCCGCGAAGGCAg < 2:442220/137‑1 (MQ=255) aaGGGCATCCCGCCGAAGGCCGTGGTTGAAGGCCCGCCCACCCGCCGCCACCCGATCCTGCTGCGCCAGACCAGCTTCAAGGCCCTGCAGGAAACGGTCGCCTTCCGCGACCAACAAGGCCGCGAAGGCAGCCacac < 7:464378/137‑1 (MQ=255) aGGGCATCCCGCCGAAGGCCGTGGTTGAAGGCCCGCCCCCCCCCCGCCACCCCATACGGCTGCGCCAGGCCAGCGTCAAGGCCCGGCCGGGAACAGTCGCCCTCCCCGACCAAAAACGCCCCGAAGGGCGCCAcccc > 4:303381/1‑137 (MQ=255) cgccgAAGGCCGTGGTTGAAGGCCCGCCCCCCCCCCGCCCCCCGAACCGGCTGCGGCCGCCCCGCTTCAAGGCCCGGGAGGAAACGGTCGCCTTCCGCGCCCCCCCACGCCGCGCAGGCCGCCCCCCCCCCCGCttt > 6:82847/1‑137 (MQ=255) tGAAGGCCCGCCCCCCCGCCGCCACCCGCTACTGCTGCGCCAGACCAGCTTCAACGCCCTGGAGGAAACGGTCGCCTTCCGCGCCCCACAACGCCGCGCAGGCAGCCACCCCGCCCGCTTTGGCGAAATAGAGCAAc > 7:15829/1‑137 (MQ=255) ggCCCGCCCACCCGCCGCGACCCGATCCTGCTGCGCCAGACCAGCTTCAAGCCCCTGCAGTAAACGGTCGCCTTCCGCGACCAACAAGGCCGCGAAGGCAGCCACACCGCACGCTTTGGCGAAATCGAGCAAcgcgg < 4:198688/137‑1 (MQ=255) cgcccACCCGCCGCCACCCGATCCTGCTGCGCCAGACCAGCTTCAAGGCCCTGCAGGAAACGGTCGCCTTCCGCGACCAACAAGGCCGCGAAGGCAGCCACACCGCACGCTTTGGCGAAATCGAGCAAcgcggcgcg < 8:463516/137‑1 (MQ=255) cgcccACCCGCCGCCACCCGATCCTGCTGCGCCAGACCAGCTTCAAGGCCCTGCAGGAAACGGTCGCCTTCCGCGACCAACAAGGCCGCGAAGGCAGCCACACCGCACGCTTTGGCGAAATCGAGCAAcgcggcgcg < 8:470315/137‑1 (MQ=255) | TCGCCGATGTGGTGGCCTTCAAGGGCCCGCACATCAACCACCTGACCCCGCGTACCCTGGATATCGATGCAATCCAGCTCGGCATGCCTGCCAAGGGCATCCCGCCGAAGGCCGTGGTTGAAGGCCCGCCCACCCGCCGCCACCCGATCCTGCTGCGCCAGACCAGCTTCAAGGCCCTGCAGGAAACGGTCGCCTTCCGCGACCAACAAGGCCGCGAAGGCAGCCACACCGCACGCTTTGGCGAAATCGAGCAACGCGGCGCG > ACB143/6002969‑6003231 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |