Predicted mutation
evidence seq id position mutation annotation gene description
RA ACB143 690,150 T→G intergenic (+27/‑2432) bauC → / → PP_RS08750 Putative 3‑oxopropanoate dehydrogenase/tRNA‑Val

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB143690,1500TG76.5% 11.7 / ‑0.9 18intergenic (+27/‑2432)bauC/PP_RS08750Putative 3‑oxopropanoate dehydrogenase/tRNA‑Val
Reads supporting (aligned to +/- strand):  ref base T (4/0);  new base G (13/0);  total (18/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.82e-01

CGGATGGTGTGCGCTTCTATACCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGAGGCCGTCA‑GG‑CC‑TCCCCGC‑TTTG‑TTTTTGGGGGT‑TGGTTGTTGCTGTTGGGGAGGAACTGTTTTGAG  >  ACB143/690023‑690211
                                                                                                                                |                                                                   
cGGATGGTGTGCGCTTCTATACCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGGCGGCA‑gggcgcc                                                           >  3:114763/1‑132 (MQ=255)
     ggTGTGCGCTTCTATACCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGTGGCCGTGA‑GC‑CC‑Gcgccgcc                                                    >  8:250686/1‑136 (MQ=255)
     ggTGTGCGCTTCTATACCCGTCGCAAGGCCATTACCAAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGAATACCCTAGCCTGTAAGGGATGTAGATGAAGC‑GGAGAGGCCGTCA‑GG‑CC‑TCCCCGcg                                                    >  6:452860/1‑136 (MQ=255)
            gcTTCTATACCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGCGCCGGAC‑GG‑CC‑CCCCCCC‑TTTG‑tttt                                           >  8:117142/1‑137 (MQ=255)
                    aCCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGGCGGGC‑GG‑CC‑GGCCCGCGTGTG‑TTTTTgggggg                                    >  6:416778/1‑136 (MQ=255)
                        gTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGCCGGGA‑GG‑CCGCCCCCCC‑TTTG‑TTTTTGGgggt‑gggt                               >  5:202670/1‑137 (MQ=255)
                           gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGGCGTCA‑CG‑CC‑GCCCCGC‑GTTGTTTTTTGGGGGT‑GGgttgt                            >  6:95204/1‑137 (MQ=255)
                           gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGGCCGCA‑GG‑CC‑CGCCCCC‑GTGG‑TTTTTGtgggt‑ggggtttt                           >  2:471824/1‑132 (MQ=255)
                           gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGGCCCGA‑GG‑CC‑CACCCCC‑CCTG‑TTTTTTTGGGT‑GTgttgtt                           >  3:199295/1‑137 (MQ=255)
                           gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGCCGGGA‑GG‑CC‑GCCCCCC‑GGTT‑TTTTTTTGGGG‑TGgttgtt                           >  5:253028/1‑137 (MQ=255)
                           gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGCCGGCA‑GG‑CC‑GCCCCGCGGTTG‑TTTTTTTGggt‑gggtttt                            >  1:268070/1‑135 (MQ=255)
                             aaGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGGGGGCCGGCG‑GC‑CC‑GCCCCGG‑GTGT‑TTTTTTGGGGG‑GGGtttttg                          >  7:59561/1‑137 (MQ=255)
                             aaGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGAGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGTGGCGGAA‑CG‑CCATCCCCCG‑GTGG‑GTTTTGGTGTT‑TGGttgttg                          >  6:72910/1‑137 (MQ=255)
                                             gTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGGGGGCCGGCG‑GGCCC‑ACCCCGG‑TGTT‑TTTTTGGGGTTGTGGGT‑TGGGTGTTGGggagggtt           >  7:202668/1‑134 (MQ=255)
                                             gTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGGCGGGA‑GG‑CC‑GCCCCGC‑GTTT‑TTTTTGGGGGT‑TGGTTGTTGGTGTTTGGGGGGGACtt         >  2:45241/1‑136 (MQ=255)
                                               tGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGGGGGGA‑GG‑CC‑GCCCCCCGGTGG‑TTTTTGGGGGT‑GGGGTTTGGGGGTTGGgggggactttt        >  2:109724/1‑132 (MQ=255)
                                               tGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGGCGGCAGGG‑CG‑CCCCCGG‑GTGG‑TTTTTGGGGG‑‑TGGGTGTTGGGGGTGGGGTGGGACGGtt       >  2:342970/1‑137 (MQ=255)
                                                    gCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGA‑GGGGAGGCCCTCC‑CG‑CC‑ACCCCCC‑CGTGTTTTTTTGGGGT‑GGGTTTTTG‑TGTTTGGGAGGTACAGTTTTgat  >  8:369248/1‑136 (MQ=255)
                                                                                                                                |                                                                   
CGGATGGTGTGCGCTTCTATACCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGAGGCCGTCA‑GG‑CC‑TCCCCGC‑TTTG‑TTTTTGGGGGT‑TGGTTGTTGCTGTTGGGGAGGAACTGTTTTGAG  >  ACB143/690023‑690211

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑