| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | ACB143 | 5,386,510 | 2 bp→TA | intergenic (‑519/+1131) | CJ019_05070 ← / ← nptII | hypothetical protein/neomycin phosphotransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB143 | 5,386,510 | 0 | A | T | 89.5% | 34.6 / ‑2.4 | 19 | intergenic (‑519/+1132) | CJ019_05070/nptII | hypothetical protein/neomycin phosphotransferase |
| Reads supporting (aligned to +/- strand): ref base A (0/2); new base T (8/9); total (8/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.85e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01 | |||||||||||
| * | ACB143 | 5,386,511 | 0 | T | A | 94.7% | 55.8 / NA | 19 | intergenic (‑520/+1131) | CJ019_05070/nptII | hypothetical protein/neomycin phosphotransferase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base A (8/10); minor base C (0/1); total (8/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCCGTCGT‑‑‑CA‑GGA‑TC‑‑ATCCGGGCGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTGGGCTCC > ACB143/5386417‑5386609 ||| tATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGTGGTG‑‑TACGGT‑ACAAACCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGaa < 7:84131/137‑1 (MQ=255) tAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGTGGTG‑‑TACGGT‑ACAAACCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCAc > 5:354002/1‑137 (MQ=255) aaaaTTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGTGGTG‑‑TACGGT‑ACAAACCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTcc < 4:441254/137‑1 (MQ=255) aaTTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGTGGTG‑‑TACGGT‑ACAAACCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCgg < 6:237848/137‑1 (MQ=255) aaTTGATCCAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGTGGTGTACG‑GGACAA‑‑ACCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCgg < 6:278733/137‑1 (MQ=255) aaTTCATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGTGGTG‑‑TACGGT‑ACAAACCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCgg < 5:275831/137‑1 (MQ=255) caaTTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGTGGTG‑‑TACGGT‑ACAAACCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCtttt < 8:101961/136‑1 (MQ=255) tttAATACCCTAATATGACATCTCGGCCGGATTGTAGACGACGGCGGTCTCAGTGGTG‑‑TACGGT‑ACAAACCCCGACGCTAGCAACGCAGGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTg < 8:34957/137‑1 (MQ=255) aaTATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGTGGTG‑‑TACGGT‑ACAAACCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAgg < 6:334671/137‑1 (MQ=255) aaTATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGTGGTG‑‑TACGGT‑ACAAACCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAgg < 7:401381/137‑1 (MQ=255) aaTATCACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGTGGTG‑‑TACGGT‑ACAAACCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAgg < 5:302790/137‑1 (MQ=255) tatGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGTGGTG‑‑TACGGT‑ACAAACCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCAGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGc > 4:304534/1‑137 (MQ=255) atGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGTGGTG‑‑TACGGT‑ACAAACCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGTGCTGCGGGTGCCAGGGCg > 5:388394/1‑137 (MQ=255) atgcccgccggcTTGTCGACGACGGCGGTCTCAGTGGTG‑‑TACTGA‑CCAAACCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGccc < 2:442809/131‑1 (MQ=255) tctcGGCCGGCTTGTCGACGACGGCGGTCTCAGTGGTG‑‑TACGGT‑ACAAACCCGGACGCTAGCAACGCATGAGAAAGGCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCt > 5:121602/1‑137 (MQ=255) ctcGGCCGGCTTGTCGACGACGGCGGTCTCAGTGGTG‑‑TACGGT‑ACAAACCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGCTCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCtt > 1:392942/1‑137 (MQ=255) ctcGGCCGGCTTGTCGACGACGGCGGTCTCAGTGGTG‑‑TACGGT‑ACAAACCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCtt > 5:178865/1‑137 (MQ=255) ggccggcTTGTCGACGACGGCGGTCTCAGTGGTG‑‑TACGGT‑ACAAACCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTggg > 4:57059/1‑137 (MQ=255) ggcTTGTCGACGACGGCGGTCTCAGTGGTG‑‑TACGGT‑ACAAACCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTTCGCTGCGGGTGCCAGGGCGTGCCCTTGGGCTcc > 5:58552/1‑137 (MQ=255) ||| TATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCCGTCGT‑‑‑CA‑GGA‑TC‑‑ATCCGGGCGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTGGGCTCC > ACB143/5386417‑5386609 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |