| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB143 | 910,666 | A→G | 38.5% | intergenic (‑50/+149) | CJ019_00872 ← / ← oprD_4 | hypothetical protein/Porin D |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB143 | 910,666 | 0 | A | G | 38.5% | 25.5 / 43.5 | 39 | intergenic (‑50/+149) | CJ019_00872/oprD_4 | hypothetical protein/Porin D |
| Reads supporting (aligned to +/- strand): ref base A (13/11); new base G (10/5); total (23/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.17e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.49e-01 | |||||||||||
AGGCAACTGCCCAGGTTGAGGATCAGCTCGGGGTGCGACCTGTAGACTGCGAAACGCGGGGATTGGGTGGGCATGCTCGCTACTGCGTGGGCAGGGCCATTCGCGGCGGCGATATTCTAGGGAAGCGGGCATTGTCGGACAAGATTTGTCCGACGGAAGCACGGTGTACCGTACTTTTCGATTTGCCGTGGACCCTTGTAGGAGCGGGTTTACCCGCGAACACCGGCGAAGCCTTCTTCGCGGGTAACC > ACB143/910543‑910791 | aggcaaCTGCCCAGGTTGAGGATCAGCTCGGGGTGCGACCTGTAGACTGCGAAACGCGGGGATTGGGTGGGCATGCTCGCTACTGCGTGGGCAGGGCCATTCGCGGCGGCGATATTCTAGGGAGGCGGGCATTGTc < 7:242304/136‑1 (MQ=255)aggcaaCTGCCCAGGTTGAGGATCAGCTCGGGGTGCGACCTGTAGACTGCGAAACGCGGGGATTAGGTGGGCATGCTCGCTACTGCGTGGGCAGGGCCATTCGCGGCGGCGATATTCTAGGGAGGCGGGCATTGTc < 3:119977/136‑1 (MQ=255) aCTGCCCAGGTTGAGGATCAGCTCGGGGTGCGACCTGTAGACTGCGAAACGCGGGGATTGGGTGTGCATGCTCGCTACTGCGTGGGCAGGGCCATTCGCGGCGGCGATATTCTAGGGAAGCGGGCATTGTCGGACa < 8:44543/136‑1 (MQ=255) ggAGCAGCTCGGGGTGCGACCTGTAGACTGCGAAACGCGGGGATTGGGGGGGCATGCTCGCTACTGCGTGGGCAGGGCCATTCGCGGCGGCGATATTCTAGGGAAGCGGGCATTGTCGGACAAGATTTGTCCGACg < 3:131927/136‑1 (MQ=255) tCAGCTCGGGGTGCGACCTGTAGACTGCGAAACGCGGGGATTGGGTGGGCATGCTCGCTACTGCGTGGGCAGGGCCATTCGCGGCGGCGATATTCTAGGGAAGCGGGCATTGTCGGACAAGATTTGTCCGACGGaa < 4:287152/136‑1 (MQ=255) aGCTCGGGGTGCGACCTGTAGACTGCGAAACGCGGGGATTGGGTGGGCATGCTCGCTACTGCGTGGGCAGGGCCATTCGCGGCGGCGATATTCTAGGGAGGCGGGCATTGTCGGACAAGATTTGTCCGACGGAAGc < 2:87422/136‑1 (MQ=255) aGCTCGGGGTGCGACCTGTAGACTGCGAAACGCGGGGATTGGGTGGGCATGCTCGCTACTGCGTGGGCAGGGCCATTCGCGGCGGCGATATTCTAGGGAAGCGGGCATTGTCGGACAAGATTTGTCCGACGGAAGc < 1:103830/136‑1 (MQ=255) aGCTCGGGGTGCGACCTGTAGACTGCGAAACGCGGGGATTGGGTGGGCATGCTCGCTACTGCGTGGGCAGGGCCATTCGCGGCGGCGATATTCTAGGGAAGCGGGCATTGTCGGACAAGATTTGTCCGACGGAAGc < 6:111374/136‑1 (MQ=255) aGCTCGGGGAGCGACCTGTAGACTGCGAAACGCGGGGATTGGGGGGGCATGCGCGCTACTGCGTGGGCAGGGCCATTCGCGGCGGCGATATTCTAGGGAAGCGGGCATTGTCGGACAAGATTTGTCCGACGGAAGc < 3:40197/136‑1 (MQ=255) tCGGGGTGCGACCTGTAGACTGCGAAACGCGGGGATTGGGTGGGCATGCTCGCTACTGCGTGGGCAGGGCCATTCGCGGCGGCGATATTCTAGGGAAGCGGGCATTGTCGGACAAGATTTGTCCGACGGaa > 6:257905/1‑131 (MQ=255) tCGGGGTGCGACCTGTAGACTGCGAAACGCGGGGATTGGGTGGGCATGCTCGCTACTGCGTGGGCAGGGCCATTCGCGGCGGCGATATTCTAGGGAAGCGGGCATTGTCGGACAAGATTTGTCCGACGGaa < 5:257905/131‑1 (MQ=255) tCGGGGTGCGACCTGTAGACTGCGAAACGCGGGGATTGGGTGGGCATGCTCGCTACTGCGTGGGCAGGGCCATTCGCGGCGGCGATATTCTAGGGAAGCGGGCATTGTCGGACAAGATTTGTCCGACGGAAGCACg > 7:35285/1‑136 (MQ=255) tGCGACCTGTAGACTGCGAAACGCGGGGATTGGGTGGGCATGCTCGCTACTGCGTGGGCAGGGCCATTCGCGGCGGCGATATTCTAGGGAGGCGGGCATTGTCGGACAAGATTTGTCCGACGGAAGCACGGTGTAc > 3:135430/1‑136 (MQ=255) gCGACCTGTAGACTGCGAAACGCGGGGATTGGGTGGGCATGCTCGCTACTGCGTGGGCAGGGCCATTCGCGGCGGCGATATTCTAGGGAAGCGGGCATTGTCGGACAAGATTTGTCCGACGGAAGCACGGTGTAcc > 6:73441/1‑136 (MQ=255) ccTGTAGACTGCGAAACGCGGGGATTGGGTGGGCATGCTCGCTACTGCGTGGGCAGGGCCATTCGCGGCGGCGATATTCTAGGGAGGCGGGCATTGTCGGACAAGATTTGTCCGACGGAAGCACGGTGTACCGTAc > 6:278834/1‑136 (MQ=255) ccTGTAGACTGCGAAACGCGGGGATTGGGTGGGCATGCTCGCTACTGCGTGGGCAGGGCCATTCGCGGCGGCGATATTCTAGGGAAGCGGGCATTGTCGGACAAGATTTGTCCGACGGAAGCACGGTGTACCGTAc > 7:242357/1‑136 (MQ=255) ccTGTAGACTGCGAAACGCGGGGATTGGGTGGGCATGCTCGCTACTGCGTGGGCAGGGCCATTCGCGGCGGCGATATTCTAGGGAAGCGGGCATTGTCGGACAAGATTTGTCCGACGGAAGCACGGTGTACCGTAc > 8:113178/1‑136 (MQ=255) cTGTAGACTGCGAAACGCGGGGATTGGGTGGGCATGCTCGCTACTGCGTGGGCAGGGCCATTCGCGGCGGCGATATTCTAGGGAAGCGGGCATTGTCGGACAAGATTTGTCCGACGGAAGCACGGTTTACCGTACt > 8:261465/1‑136 (MQ=255) gACTGCGAAACGCGGGGATTGGGTGGGCATGCTCGCTACTGCGTGGGCAGGGCCATTCGCAGCGGCGATATTCTAGGGAAGCGGGCATTGTCGGACAAGATTTGTCCGACGGAAGCACGGTGTACCGTACTTTTc > 7:180337/1‑135 (MQ=255) gAAACGCGGGGATTGGGTGGGCATGCTCGCTACTGCGTGGGCAGGGCCATTCGCGGCGGCGAGATTCTAGGGAGGCGGGCATTGTCGGACAAGATTTGTCCGACGGAAGCACGGTGTACCGTACTTTTCGATTTGc > 1:17188/1‑136 (MQ=255) aaaCGCGGGGATTGGGTGGGCATGCTCGCTACTGCGTGGGCAGGGCCATTCGCGGCGGCGATATTCTAGGGAAGCGGGCATTGTCGGACAAGATTTGTCCGACGGAAGCACGGTGTACCGTACTTTTCGATTTGcc < 2:145543/136‑1 (MQ=255) aaCGCGGGGATTGGGTGGGCATGCTCGCTACTGCGTGGGCAGGGCCATTCGCGGCGGCGATATTCTAGGGAGGCGGGCATTGTCGGACAAGATTTGTCCGACGGAAGCACGGTGTACCGTACTTTTCGATTTGCCg > 8:225984/1‑136 (MQ=255) aaCGCGGGGATTGGGTGGGCATGCTCGCTACTGCGTGGGCAGGGCCATTCGCGGCGGCGATATTCTAGGGAGGCGGGCATTGTCGGACAAGATTTGTCCGACGGAAGCACGGTGTACCGTACTTTTCGATTTGCCg > 2:175087/1‑136 (MQ=255) aaCGCGGGGATTGGGTGGGCATGCTCGCTACTGCGTGGGCAGGGCCATTCGCGGCGGCGATATTCTAGGGAGGCGGGCATTGTCGGACAAGATTTGTCCGACGGAAGCACGGTGTACCGTACTTTTCGATTTGCCg < 3:167192/136‑1 (MQ=255) aaCGCGGGGATTGGGTGGGCATGCTCGCTACTGCGTGGGCAGGGCCATTCGCGGCGGCGATATTCTAGGGAAGCGGGCATTTTCCGACAACATTTGTCCGACCGAAGCACCGTGGACCGGACTTTTCGATTTGCCg > 8:105318/1‑136 (MQ=255) ttGGGTGGGCATGCTCGCTACTGCGTGGGCAGGTCCATTCGCGGCGGCGATATTCTAGGGAGGCGGGCATTGTCGGACAAGATTTGTCCGACGGAAGCACGGTGTACCGTACTTTTCGATTTGCCGTGGACCCTTg > 5:84040/1‑136 (MQ=255) aTGCTCGCTACTGCGTGGGCAGGGCCATTCGCGGCGGCGATATTCTAGGGAGGCGGGCATTGTCGGACAAGATTTGTCCGACGGAAGCACGGTGTACCGTACTTTTCGATTTGCCGTGGACCCTTGTAGGAGCggg > 1:189066/1‑136 (MQ=255) gCTCGCTACTGCGTGGGCAGGGCCATTCGCGGCGGCGATATTCTAGGGAAGCGGGCATTGTCGGACAAGATTTGTCCGACGGAAGCACGGTGTACCGTACTTTTCGATTTGCCGTGGACCCTTGTAGGAGCGGGtt > 6:44991/1‑136 (MQ=255) gggCAGGGCCATTCGCGGCGGCGATATTCTAGGGAAGCGGGCATTGTCGGACAAGATTTGTCCGACGGaa < 5:244058/70‑1 (MQ=255) gggCAGGGCCATTCGCGGCGGCGATATTCTAGGGAAGCGGGCATTGTCGGACAAGATTTGTCCGACGGaa > 6:244058/1‑70 (MQ=255) ggCAGGGCCATTCGCGGCGGCGATATTCTAGGGAAGCGGGCATTGTCGGACAAGATTTGTCCGACGGAAGCACGGTGTACCGTACTTTTCGATTTGCCGTGGACCCTTGTAGGCGCGGGTTTACCCGCGAACACCg > 1:42104/1‑136 (MQ=255) cATTCGCGGCGGCGATATTCTAGGGAGGCGGGCATTGTCGGACAAGATTTGTCCGACGGAAGCACGGTGTACCGTACTTTTCGATTTGCCGTGGACCCTTGTAGGCGCGGGTTTTCCCGCGAACACCGGCGCAGcc > 4:279841/1‑136 (MQ=255) aTTCGCGGCGGCGATATTCTAGGGAAGCGGGCATTGTCGGACAAGATTTGTCCGACGGAAGCACGGTGTACCGTACTTTTCGATTTGCCGTGGACCCTTGTAGGAGCGGGTTTACCCGCGGACACCGGCGCAGccg > 3:134279/1‑135 (MQ=255) gcggcggcgATATTCTAGGGAGGCGGGCATTGTCGGACAAGATTTGTCCGACGGAAGCACGGTGTACCGTACTTTTCGATTTGCCGTGGACCCTTGTAGGAGCGGGTTTACCCGCGAACACCGGCGAAGCcttct > 7:52504/1‑135 (MQ=255) cgATATTCTAGGGAGGCGGGCATTGTCGGACAAGATTTGTCCGACGGAAGCACGGTGTACCGTACTTTTCGATTTGCCGTGGACCCTTGTAGGAGCCGGTTTAACCGCGAACACCCGCGAAGCCTTATTCGCggg > 6:93141/1‑135 (MQ=255) cgATATTCTAGGGAAGCGGGCATTGTCGGACAAGATTTGTCCGACGGAAGCACGGTGTACCGTACTTTTCGATTTGCCGTGGACCCTTGTAGGAGCGGGTTTACCCGCGAACACCGGCGAAGCCTTCTTCGCGGGt < 7:284847/136‑1 (MQ=255) cgATATTCTAGGGAAGCGGGCATTGTCGGACAAGATTTGTCCGACGGAAGCACGGTGTACCGTACTTTTCGATTTGCCGTGGACCCTTGTAGGAGCGGGTTTACCCGCGAACACCGGCGAAGCCTTCTTCGCGGGt < 1:234026/136‑1 (MQ=255) cgATATTCTAGGGAAGCGGGCATTGTCGGACAAGATTTGTCCGACGGAAGCACGGTGTACCGTACTTTTCGATTTGCCGTGGACCCTTGTAGGAGCGGGTTTACCCCCGAACACCCCCCAAGACGTCTTAGCggg > 3:74708/1‑135 (MQ=255) ttCTAGGGAGGCGGGCATTGTCGGACAAGATTTGTCCGACGGAAGCACGGTGTACCGTACTTTTCGATTTGCCGTGGACCCTTGTAGGAGCGGGTTTACCCGCGAACACCGGCGAAGCCTTCTTCGCGGGTAAcc < 3:274759/135‑1 (MQ=255) aaGCGGGCATTGTCGGACAAGATTTGTCCGACGGaa < 7:232642/36‑1 (MQ=255) aaGCGGGCATTGTCGGACAAGATTTGTCCGACGGaa > 8:232642/1‑36 (MQ=255) | AGGCAACTGCCCAGGTTGAGGATCAGCTCGGGGTGCGACCTGTAGACTGCGAAACGCGGGGATTGGGTGGGCATGCTCGCTACTGCGTGGGCAGGGCCATTCGCGGCGGCGATATTCTAGGGAAGCGGGCATTGTCGGACAAGATTTGTCCGACGGAAGCACGGTGTACCGTACTTTTCGATTTGCCGTGGACCCTTGTAGGAGCGGGTTTACCCGCGAACACCGGCGAAGCCTTCTTCGCGGGTAACC > ACB143/910543‑910791 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |