Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 2,175,073 A→G 6.5% intergenic (‑8/‑174) CJ019_02064 ← / → CJ019_02065 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1432,175,0730AG 6.5% 456.2 / 15.2 139intergenic (‑8/‑174)CJ019_02064/CJ019_02065hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (63/67);  new base G (3/6);  total (66/73)
Fisher's exact test for biased strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.86e-01

Only 100 of 145 total aligned reads displayed.

GAGTACTCACCTTTCTCCAAATTTCTGATGTACCTGACGCTGGCGGCAGGATCAAAAGCTTCCTGCGTAAGGCCTCGCTGAACACGCAGTTGGCGGAATACGCGGCCAAAGGCAATTTTGTAATTCATCACGCGTACGCGTCCAAAAGGAAGCATTCAACCTATTGCGTCGAGGCACGAACAGGCAGTATGCTGCCGGTTACGTCATTTTCTGCGTTTTCGCCTTTCCGAAATCTGAGTGCTCCCGCCTTTAGAGAGTCCTCGGGAA  >  ACB143/2174938‑2175204
                                                                                                                                       |                                                                                                                                   
gagTACTCACCTTTCTCCAAATTTCTGATGTACCTGACGCTGGCGGCAGGATCAAAAGCTTCCTGCGTAAGGCCTCGCTGAACACGCAGTTGGCGGAATACGCGGACAAAGGCAATTTTGTAATTCATCacgcgta                                                                                                                                     >  3:144349/1‑136 (MQ=255)
      tCACCTTTCTCCAAATTTCTGATGTACCTGACGCTGGCGGCAGGATCAAAAGCTTCCTGCGTAAGGCCTCGCTGAACACGCAGTTGGCGGAATACGCGGCCAAAGGCAATTTTGTAATTCATCACGCGTACGCGTc                                                                                                                               >  5:208314/1‑136 (MQ=255)
      tCACCTTTCTCCAAATTTCTGATGTACCTGACGCTGGCGGCAGGATCAAAAGCTTCCTGCGTAAGGCCTCGCTGAACACGCAGTTGGCGGAATACGCGGCCAAAGGCAATTTTGTAATTCATCACGCGTACGCGTc                                                                                                                               >  3:291405/1‑136 (MQ=255)
      tCACCTTTCTCCAAATTTCTGATGTACCTGACGCTGGAGGCAGGATAAAAAGCTTCCTGCGTAAGGCCTCCCTGAACACGCAGTAGGCGGAAAACGCGGCCAAAGGCAATTTAGTAATTAATCacgcgtacgctac                                                                                                                               >  6:215033/1‑133 (MQ=255)
      tCACCTTTCTCCAAATTTCTGATGTACCTGAAGCTGGCGGCAGGATCAAAAGCTTCCTGCGTAAGGACTCGCTGCACACGCGGTTGGCGGAATACGCGGCAAAAGGCAATTTTGTAATTAATCACGCGTACGCGTc                                                                                                                               >  1:75050/1‑136 (MQ=255)
        aCCTTTCTCCAAATTTCTGATGTACCTGACGCTGGCGGCAGGATCAAAAGCTTCCTGCGTAAGGCCTCGCTGAACACGCAGTTGGCGGAATACGCGGCCAAAGGCAATTTTGTAATTCATCACGCGTACGCGTcc                                                                                                                              <  1:101422/135‑1 (MQ=255)
        aCCTTTCTCCAAATTTCTGATGTACCTGACGCTGGCGGCAGGATCAAAAGCTTCCTGCGTAAGGCCTCGCTGAACACGCAGTTGGCGGAATACGCGGCCAAAGGCAATTTTGTAATTCATCACGCGTACGCGTcc                                                                                                                              >  1:50925/1‑135 (MQ=255)
           tttCTCCAAATTTCTGATGTACCTGACGCTGGCGGCAGGATCAAAAGCTTCCTGCGTAAGGCCTCGCTGAACACGCAGTTGGCGGAATACGCGGCCAAAGGCAATTTTGTAATTCATCACGCGTACGCGTCaaa                                                                                                                            >  8:220692/1‑134 (MQ=255)
           tttCTCCAAATTTCTGATGTACCTGACGCTGGCGGCAGGATCAAAAGCTTCCTGCGTAAGGCCTCGCTGAACACGCAGTTGGCGGAATACGCGGCCAAAGGCAATTTTGTAATTCATCACGCGTACGCGTCCaa                                                                                                                            >  5:47823/1‑134 (MQ=255)
             tctcCAAATTTCTGATGTACCTGACGCTGGCGGCAGGATCAAAAGCTTCCTGCGTAAGGCCTCGCTGAACACGCAGTTGGCGGAATACGCGGCCAAAGGCAATTTTGTAATTCATCACGCGTACGCGTCCaaaa                                                                                                                          <  5:147402/134‑1 (MQ=255)
             tctcCAAATTTCTGATGTACCTGACGCTGGCGGCAGGATCAAAAGCTTCCTGCGTAAGGCCTCGCTGAACACGCAGTTGGCGGAATACGCGGCCAAAGGCAATTTTGTAATTCATCACGCGTACGCGTCCAAAAg                                                                                                                         <  3:186496/135‑1 (MQ=255)
                ccAAATTTCTGATGTACCTGACGCTGGCGGCAGGATCAAAAGCTTCCTGCGTAAGGCCTCGCTGAACACGCAGTTGGCGGAATACGCGGCCAAAGGCAATTTTGTAATTCATCACGCGTACGCGTCCAAAAGGAAg                                                                                                                     >  1:311924/1‑136 (MQ=255)
                ccAAATTTCTGATGTACCTGACGCTGGCGGCAGGATCAAAAGCTTCCTGCGTAAGGCCTCGCTGAACACGCAGTTGGCGGAATACGCGGCCAAAGGCAATTTTGTAATTCATCACGCGTACGCGTCCAAAAGGAAg                                                                                                                     >  1:140850/1‑136 (MQ=255)
                ccAAATTTCTGATGTACCTGACGCTGGCGGCAGGATCAAAAGCTTCCTGCGTAAGGCCTCGCTGAACACGCAGTTGGCGGAATACGCGGCCAAAGGCAATTTTGTAATTCATCACGCGTACGCGTCCAAAAGGAAg                                                                                                                     >  7:10303/1‑136 (MQ=255)
                ccAAATTTCTGATGTACCTGACGCTGGCGGCAGGATCAAAAGCTTCCTGCGTAAGGCCTCGCTGAACACGCAGTTGGCGGAATACGCGGCCAAAGGCAATTTTGTAATTCATCACGCGTACGCGTCCAAAAGGAAg                                                                                                                     >  7:186267/1‑136 (MQ=255)
                ccAAATTTCTGATGTACCTGACGCTGGCGGCAGGATCAAAAGCTTCCTGCGTAAGGCCTCGCTGAACACGCAGTTGGCGGAATACGCGGCCAAAGGCAATTTTGTAATTCATCACGCGTACGCGTCCAAAAGGAAg                                                                                                                     >  2:202297/1‑136 (MQ=255)
                ccAAATTTCTGATGTACCTGACGCTGGCGGCAGGATCAAAAGCTTCCTGCGTAAGGCATCGCTGAACACGCAGTTGGCGGAATACGCGGCCAAAGGCAATTTTGTAATTCATCACGCGTACGCGTCCAAAAGGAAg                                                                                                                     >  3:252268/1‑136 (MQ=255)
                ccAAATTTCTGATGTACCTGACGCTGGCGGCAGGATCAAAAGCTTCCTGCGAAAGGCCTCGCTGAACACGCAGTTTGCGGAATACGCGGCCAAAGGCAATTTTGTAATTCATCACGCTTACGCGTCCAAAAGGaat                                                                                                                     >  4:80477/1‑135 (MQ=255)
                 cAAATTTCTGATGTACCTGACGCTGGCGGCAGGATCAAAAGCTTCCTGCGTAAGGCCTCGCTGAACACGCAGTTGGCGGAATACGCGGCCAAAGGCAATTTTGTAATTCATCACGCGTACGCGTCCAAAAGGAAg                                                                                                                     <  6:104318/135‑1 (MQ=255)
                 cAAATTTCTGATGTACCTGACGCTGGCGGCAGGATCAAAAGCTTCCTGCGTAAGGCCTCGCTGAACACGCAGTTGGCGGAATACGCGGCCAAAGGCAATTTTGTAATTCATCACGCGTACGCGTCCAAAAGGAAGc                                                                                                                    <  1:308752/136‑1 (MQ=255)
                   aaTTTCTTATGTACCTGACGCTTGCGGCAGGATGAAAAGCTTCCTGCGTTAGGCCTCGCTGAACACGCAGTTGGCGGAATACGCGGCCAAAGGGAATTTTGTAATTCATCACGCGTACGCTTCCAAAAGGAAGCAt                                                                                                                  <  4:189482/136‑1 (MQ=255)
                   aaTTTCTGATGTACCTGACGCTGGCGGCAGGATCAAAAGCTTCCTGCGTAAGGCCTCGCTGAACACGCAGTTGGCGGAATACGCGGCCAAAGGCAATTTTGTAATTCATCACGCGTACGCGTCCAAAAGGGAGCAt                                                                                                                  <  8:221746/136‑1 (MQ=255)
                           aTGTACCTGACGCTGGCGGCAGGATCAAAAGCTTCCTGCGTAAGGCCTCGCTGAACACGCAGTTGGCGGAATACGCGGCCAAAGGCAATTTTGTAATTCATCACGCGTACGCGTCCAAAAGGAAGCATTCAAc                                                                                                             <  7:20755/133‑1 (MQ=255)
                           aTGTACCTGACGCTGGCGGCAGGATCAAAAGCTTCCTGCGTAAGGCCTCGCTGAACACGCAGTTGGCGGAATACGCGGCCAAAGGCAATTTTGTAATTCATCACGCGTACGCGTCCAAAAGGAAGCATTCAAc                                                                                                             <  5:259272/133‑1 (MQ=255)
                            tGTACCTGACGCTGGCGGCAGGATCAAAAGCTTCCTGCGTAAGGCCTCGCTGAACACGCAGTTGGCGGAATACGCGGCCAAAGGCAATTTTCTAATTCATCACGCGTACGCCTCCAAAAGGAAGCATTAAAACTAt                                                                                                         >  3:291457/1‑136 (MQ=255)
                                ccTGACGCTGGCGGCAGGATCAAAAGCTTCCTGCGTAAGGCCTCGCTGAACACGCAGTTGGCGGAATACGCGGCCAAAGGCAATTTTGTAATTCATCACGCGTACGCGTCCAAAAGGAAGCATTCAACCTATTGc                                                                                                      <  4:9173/135‑1 (MQ=255)
                                ccTGACGCTGGCGGCAGGATCAAAAGCTTCCTGCGTAAGGCCTCGCTGAACACGCAGTTGGCGGAATACGCGGCCAAAGGCAATTTTGTAATTCATCACGCGTACGCGTCCAAAAGGAAGCATTCAACCTATTGc                                                                                                      <  4:205253/135‑1 (MQ=255)
                                   gACGCTGGCGGCAGGATCAAAAGCTTCCTGCGAAAGGCCTCGCTGAACACGCAGTTGGCGGAATACGCGGCCAAAGGCAATTTTGTAATTCATCACGCGTACGCGTCCAAAAGGAAGCATTCAACCTATTGCGTc                                                                                                   >  6:213794/1‑135 (MQ=255)
                                    aCGCTGGCGGCAGGATCAAAAGCTTCCTGCGTAAGGCCTCGCTGAACACGCAGTTGGCGGAATACGCGGCCAAAGGCAATTTTGTAATTCATCACGCGTACGCGTCCAAAAGGAACATTCAACCTATTGCGTCGAg                                                                                                <  4:307177/136‑1 (MQ=255)
                                       cTGGCGGCAGGATCAAAAGCTTCCTGCGTAAGGCCTCGCTGAACACGCAGTTGGCGGAATACGCGGCCAAAGGCAATTTTGTAATTCATCACACGTACGCGTCCAAAAGGAAGCATTCAACCTATTGCGTCGAGGc                                                                                              <  5:40091/136‑1 (MQ=255)
                                        tGGCGGCAGGATCAAAAGCTTCCTGCGTAAGGCCTCGCTGAACACGCAGTTGGCGGAGTACGCGGCCAAAGGCAATTTTGTAATTCATCACGCGTACGCGTCCAAAAGGAAGCATTCAACCTATTGCGTCGAGGCa                                                                                             >  8:178629/1‑136 (MQ=255)
                                        tGGCGGCAGGATCAAAAGCTTCCTGCGTAAGGCCTCGCTGAACACGCAGTTGGCGGAATACGCGGCCAAAGGCAATTTTGTAATTCATCACGCGTACGCGTCCAAAAGGAAGCATTCAACCTATTGCGTCGAGGCa                                                                                             >  6:29084/1‑136 (MQ=255)
                                          gcggcAGGATCAAAAGCTTCCTGCGTAAGGCCTCGCTGAACACGCAGTTGGCGGAATACGCGTCCAAAGGCAATTTTGTAATTCATCACGCGTACGCGTCCAAAAGGAAGCATTCAACCTATTGCGTCGAGGCAc                                                                                            <  1:282160/135‑1 (MQ=255)
                                          gcggcAGGATCAAAAGCTTCCTGCGTAAGGCCTCGCTGAACACGCAGTTGGCGGAATACGCGGCCAAAGGCAATTTTGTAATTCATCACGCGTACGCGTCCAAAAGGAAGCATTCAACCTATTGCGTCGAGGCACg                                                                                           <  1:286999/136‑1 (MQ=255)
                                          gcggcAGGATCAAAAGCTTCCTGCGTAAGGCCTCGCTGAACACGCAGTTGGCGGAATACGCGGCCAAAGGCAATTTTGTAATTCATCACGCGTACGCGTCCAAAAGGAAGCATTCAACCTATTGCGTCGAGGCACg                                                                                           <  5:64057/136‑1 (MQ=255)
                                          gcggcAGGATCAAAAGCTTCCTGCGTAAGGCCTCGCTGAACACGCAGTTGGCGGAATACGCGGCCAAAGGCAATTTTGTAATTCATCACGCGTACGCGTCCAAAAGGAAGCATTCAACCTATTGCGTCGAGGCACg                                                                                           <  4:14656/136‑1 (MQ=255)
                                          gcggcAGGATCAAAAGCTTCCTGCGTAAGGCCTCGCTGAACACGCAGTTGGCGGAATACGCGGCCAAAGGCAATTTTGTAATTCATCACGCGTACGCGTCCAAAAGGAAGCATTCAACCTATTGCGTCGAGGCACg                                                                                           <  2:269859/136‑1 (MQ=255)
                                           cggcAGGATCAAAAGCTTCCTGCGTAAGGCCTCGCTGAACACGCAGTTGGCGGAATACGCGGCCAAAGGCAATTTTGTAATTCATCACGCGTACGCGTCCATAAGGAAGCATTCAACCTATTGCGTCGAGGCACg                                                                                           >  4:281430/1‑135 (MQ=255)
                                            ggcAGGATCAAAAGCTTCCTGCGTAAGGCCTCGCTGAACACGCAGTTGGCGGAATACGCGGCCAAAGGCAATTTTGTAATTCATCACGCGTACGCGTCCAAAAGGAAGCATTCAACCTATTGCGTCGAGGCACGag                                                                                         >  4:23391/1‑135 (MQ=255)
                                            ggcAGGATCAAAAGCTTCCTGCGTAAGGCCTCGCTGAACACGCAGTTGGCGGAATACGCGGCCAAAGGCAATTTTGTAATTCATCACGCGTACGCGTCCAAAAGGAAGCATTCAACCTATTGCGTCGAGGCACGaa                                                                                         >  1:255651/1‑136 (MQ=255)
                                             gcAGGATCAAAAGCTTCCTGCGTAAGGCCTCGCTGAACACGCAGTTGGCGGAATACGCGGCCAAAGGCAATTTTGTAATTCATCACGCGTACGCGTCCAAAAGGAAGCATTCAACCTATTGCGTCGAGGCACGaa                                                                                         >  7:8494/1‑135 (MQ=255)
                                             gcAGGATCAAAAGCTTCCTGCGTAAGGCCTCGCTGAACACGCAGTTGGCGGAATACGCGGCCAAAGGCAATTTTGTAATTCATCACGCGTACGCGTCCAAAAGGAAGCATTCAACCTATTGCGTCGAGGCACGAAc                                                                                        <  6:152065/136‑1 (MQ=255)
                                             gcAGGATCAAAAGCTTCCTGCGTAAGGCCTCGCTGAACACCCAGTTGGCGGAATACGCGGCCAAAGGCAATTTTGTAATTCATCACGCGTACGCGTCCAAAAGGAAGCATTCAACCTATTGCGTCGAGGCACGAAc                                                                                        >  5:226363/1‑136 (MQ=255)
                                                 taTCAAAAGCTTACTGCGTAAGGCCTCGCTGAACACGCAGTTTGCGGAATACGCGGCCAAAGGCAATTTTGTAATTAATCACTCGTACGAGTCCAAAAGGAAGCATTCAACCTATTGAGTCGAGGCAAGAACAGGc                                                                                    <  7:117642/135‑1 (MQ=255)
                                                 gATCAAAAGCTTCCTGCGTAAGGCCTCGCTGAACACGCAGTTGGCGGAATACGCGGCCAAAGGCAATTTTGTAATTCATCACGCGTACGCGTCCAAAAGGAAGCATTCAACCTATTGCGTCGAGGCACGAACAGGc                                                                                    <  7:146995/136‑1 (MQ=255)
                                                   tCAAAAGCTTCCTGCGTAAGGCCTCGCTGAACACGCAGTTGGCGGAATACGCGGCCAAAGGCAATTTTGTAATTCATCACGCGTACGCGTCCAAAAGGAAGCATTCAACCTATTGCGTCGAGGCACGAACAGGCAg                                                                                  >  2:239639/1‑136 (MQ=255)
                                                     aaaaGCTTCCTGCGTAAGGCCTCGCTGAACACGCAGTTGGCGGAATACGCGGCCAAAGGCAATTTTGTAATTCATCACGCGTACGCGTCCAAAAGGAAGCATTCAACCTATTGCGTCGAGGCACGAACAGGCAGt                                                                                 <  2:256114/135‑1 (MQ=255)
                                                      aaaGCTTCCTGCGTAAGGCCTCGCTGAACACGCAGTTGGCGGAATACGCGGCCAAAGGCAATTTTGTAATTCATCACGCGTACGCGTCCAAAAGGAAGCATTCAACCTATTGCGTCGAGGCACGAACAGGCAGTAt                                                                               <  7:62290/136‑1 (MQ=255)
                                                              cTGCGTAAGGCCTCGCTGAACACGCAGTTGGCGGAATACGCGGCCAAAGGCAATTTTGTAATTCATCACGCGTACGCGTCCAAAAGGAAGCATTCAACCTATTGCGTCGAGGCACGAACAGGCAGTATGCTGCCgg                                                                       >  3:232716/1‑136 (MQ=255)
                                                               tGCGTAAGGCCTCGCTGAACACGCAGTTGGCGGAATACGCGGCCAAAGGCAATTTTGTAATTCATCACGCGTACGCGTCCaaa                                                                                                                           <  6:209740/83‑1 (MQ=255)
                                                               tGCGTAAGGCCTCGCTGAACACGCAGTTGGCGGAATACGCGGCCAAAGGCAATTTTGTAATTCATCACGCGTACGCGTCCAAAAGGAAGCATTCAACCTATTGCGTCGAGGCACGAACAGGCAGTATGCTGCCGGt                                                                      >  6:308599/1‑136 (MQ=255)
                                                               tGCGTAAGGCCTCGCTGAACACGCAGTTGGCGGAATACGCGGCCAAAGGCAATTTTGTAATTCATCACGCGTACGCGTCCAAAAGGAAGCATTCAACCTATTGCGTCGAGGCACGAACAGGCAGTATGCTGCCGGt                                                                      >  1:55499/1‑136 (MQ=255)
                                                                gCGTAAGGCCTCGCTGAACACGCAGTTGGCGGAATACGCGGCCAAAGGCAATTTTGTAATTCATCACGCGTACGCGTCCAAAAGGAAGCATTCAACCTATTGCGTCGAGGCACGAACAGGCAGTATGCTGCCGGt                                                                      >  5:39238/1‑135 (MQ=255)
                                                                     aGGCCTCGCTGAACACGCAGTTGGCGGAATACGCGGCCAAAGGCAATTTTGTAATTCATCACGCGTACGCGTCCAAAAGGAAGCATTCAACCTATTGCGTCGAGGCACGAAc                                                                                        >  1:236136/1‑112 (MQ=255)
                                                                      ggCCTCGCTGAACACGCAGTTGGCGGAATACGCGGCCAAAGGCAATTTTGTAATTCATCACGCGTACGCGTCCAAAAGGAAGCATTCAACCTATTGCGTCGAGGCACGAACAGGCAGTATGCTGCCGGTTACGTCa                                                               >  3:219455/1‑136 (MQ=255)
                                                                      ggCCTCGCTGAACACGCAGTTCGCGGAATACGCGGCCAAAGGCAATTTTGTAATTAATCACGCGTGCGCGTCCAAAAGGAAGCAGTCAACCTATTTCGTCGCGGCTCGAACAGGAAGTATGCTGCGGGTTAGGTCa                                                               >  2:236483/1‑136 (MQ=255)
                                                                       gCCTCGCTGAACACGCAGTTGGCGGAATACGCGGCCAAAGGCAATTTTGTAATTCATCACGCGTACGCGTCCAAAAGGAAGCATTCAACCTATTGCGTCGAGGCACGAACAGGCAGTATGCTGCCGGTTACGTCAt                                                              >  6:191887/1‑136 (MQ=255)
                                                                         cTCGCTGAACACGCAGTTGGCGGAATACGCGGCCAAAGGCAATTTTGTAATTCATCACGCGTACGCGTCCAAAAGGAAGCATTCAACCTATTGCGTCGAGGCACGAACAGGCAGTATGCTGCCGGTTACGTCAttt                                                            <  2:311924/136‑1 (MQ=255)
                                                                              tGAACACGCAGTTGGCGGAATACGCGGCCAAAGGCAATTTTGTAATTCATCACGCGTACGCGTCCAAAAGGAAGCAt                                                                                                                  <  2:261359/77‑1 (MQ=255)
                                                                              tGAACACGCAGTTGGCGGAATACGCGGCCAAAGGCAATTTTGTAATTCATCACGCGTACGCGTCCAAAAGGAAGCATTCAACCTATTGCGTCGAGGCACGAACAGGCAGTATGCTGCCGGTTACGTCATTTTCTGc                                                       >  7:295858/1‑136 (MQ=255)
                                                                              tGAACACGCAGTTGGCGGAATACGCGGCCAAAGGCAATTTTGTAATTCATCACGCGTACGCGTCCAAAAGGAAGCATTCAACCTATTGCGTCGAGGCACGAACAGGCAGTATGCTGCCGGTTACGTCATTTTCTGc                                                       >  5:122962/1‑136 (MQ=255)
                                                                                    cGCAGTTGGCGGAATACGCGGCCAAAGGCAATTTTGTAATTCATCACGCGTACGCGTCAAAAAGGAAGCATTCAACCTATTGCGTCGAGGCACGAACAGGCAGTATGCTGCCGGTTACGTCATTTTCTGCGTTTTc                                                 <  6:208314/136‑1 (MQ=255)
                                                                                     gCAGTAGGCGGAATACCCGGCCAAACGCAATTTTGAAATTCATCACGCGAACGCGTCCAAAAGGAAGCATTCAACCTATTCCGTCGAGGCACGAACAGGCAGTATGCTGCCGGTAACGTAATTTTCTGCGATTTct                                                >  2:153385/1‑135 (MQ=255)
                                                                                         ttGGCGGAATACGCGGCCAAAGGCAATTTTGTAATTCATCACGCGTACGCGTCCAAAAGGAAGCATTCAACCTATTGCGTCGAGGCACGAACAGGCAGTATGCTGCCGGTGACGTCATTTTCTGCGTTTTCGCCtt                                            >  1:203612/1‑136 (MQ=255)
                                                                                          tGGCGGAATACGCGGCCAAAGGCAATTTTGTAATTCATCACGCGTACGCGTCCaaa                                                                                                                           <  5:21121/56‑1 (MQ=255)
                                                                                           ggCGGAATACGCGGCCAAAGGCAATTTTGTAATTCATCACGCGTGCGCGTCCAAAAGGAAGCATTCAACCTATTGCGTCGAGGCACGAACAGGCAGTATGCTGCCGGTTACGTCATTTTCTGCGTTTTCGCCttt                                           <  7:111722/135‑1 (MQ=255)
                                                                                           ggCGGAATACGCGGCCAAAGGCAATTTTGTAATTCATCACGCGTACGCGTCCAAAAGGAAGCATTCAACCTATTGCGTCGAGGCACGAACAGGCAGTATGCTGCCGGTTACGTCATTTTCTGCGTTTTCGCCttt                                           <  1:138379/135‑1 (MQ=255)
                                                                                           ggCGGAATACGCGGCCAAAGGCAATTTTGTAATTCATCACGCGTACGCGTCCAAAAGGAAGCATTCAACCTATTGCGTCGAGGCACGAACAGGCAGTATGCTGCCGGTTACGTCATTTTCTGCGTTTTCGCCTTTc                                          >  1:214776/1‑136 (MQ=255)
                                                                                           ggCGGAATACGCGGCCAAAGGCAATTTTGTAATTCATCACGCGTACGCGTCCAAAAGGAAGCATTCAACCTATTGCGTCGAGGCACGAACAGGCAGTATGCTGACGGTTACGTCATTTTCTGCGTTTTCGCCttt                                           <  5:31662/135‑1 (MQ=255)
                                                                                                  tACGCGGCCAAAGGCAATTTTGTAATTCATCACGCGTGCGCGTCCAAAAGGAAGCATTCAACCTATTGCGTCGAGGCACGAACAGGCAGTATGCTGCCGGTTACGTCATTTTCTGCGTTTTCGCCTTTCCGAAATc                                   <  5:175930/136‑1 (MQ=255)
                                                                                                  tACGCGGCCAAAGGCAATTTTGTAATTCATCACGCGTACGCGTCCAAAAGGAAGCATTCAACCTATTGCGTCGAGGCACGAACAGGCAGTATGCTGCCGGTTACGTCATTTTCTGCGTTTTCGCCTTTCCGAAATc                                   <  2:19965/136‑1 (MQ=255)
                                                                                                    cgcgGCCAAAGGCAATTTTGTAATTCATCACGCGTACGCGTCCAAAAGGAAGCATTCAACCTATTGCGTCGAGGCACGAACAGGCAGTATGCTGCCGGTTACGTCATTTTCTGCGTTTTCGCCTTTCCGAAATCt                                  <  8:55649/135‑1 (MQ=255)
                                                                                                    cgcgGCCAAAGGCAATTTTGTAATTCATCACGCGTACGCGTCCAAAAGGAAGCATTCAACCTATAGCGTCGAGGCACGAACAGGCAGTATGCTGCCGGGTACGTCATTTTCTGCGTTTTCGCCTTTCCGAAATCt                                  >  1:159154/1‑135 (MQ=255)
                                                                                                         ccAAAGGCAATTTTGTAATTCATCACGCGTGCGCGTCCAAAAGGAAGCATTCAACCTATTGCGTCGAGGCACGAACAGGCAGTATGCTGCCGGTTACGTCATTTTCTGCGTTTTCGCCTTTCCGAAATCTGAGTGc                            <  5:69842/136‑1 (MQ=255)
                                                                                                         ccAAAGGCAATTTTGTAATTCATCACGCGTACGCGTCCAAAAGGAAGCATTCAACCTATTGCGTCGAGGCACGAACAGGCAGTATGCTGCCGGTTACGTCATTTTCTGCGTTTTCGCCTTTCCGAAATCTGAGTGc                            <  5:141298/136‑1 (MQ=255)
                                                                                                         ccAAAGGCAATTTTGTAATTCATCACGCGTACGCGTCCAAAAGGAAGCATTCAACCTATTGCGTCGAGGCACGAACAGGCAGTATGCTGCCGGTTACGTCATTTTCTGCGTTTTCGCCTTTCCGAAATCTGAGTGc                            <  5:294138/136‑1 (MQ=255)
                                                                                                         ccAAAGGCAATTTTGTAATTCATCACGCGTACGCGTCCAAAAGGAAGCATTCAACCTATTGCGTCGAGGCACGAACAGGCAGTATGCTGCCGGTTACGTCATTTTCTGCGTTTTCGCCTTTCCGAAATCTGAGTGc                            <  6:4467/136‑1 (MQ=255)
                                                                                                         ccAAAGGCAATTTTGTAATTCATCACGCGTACGCGTCCAAAAGGAAGCATTCAACCTATTGCGTCGAGGCACGAACAGGCAGTATGCTGCCGGTTACGTCATTTTCTGCGTTTTCGCCTTTCCGAAATCTGAGTGc                            <  6:65114/136‑1 (MQ=255)
                                                                                                         ccAAAGGCAATTTTGTAATTCATCACGCGTACGCGTCCAAAAGGAAGCATTCAACCTATTGCGTCGAGGCACGAACAGGCAGTATGCTGCCGGTTACGTCATTTTCTGCGTTTTCGCCTTTCCGAAATCTGAGTGc                            <  7:85812/136‑1 (MQ=255)
                                                                                                         ccAAAGGCAATTTTGTAATTCATCACGCGTACGCGTCCAAAAGGAAGCATTCAACCTATTGCGTCGAGGCACGAACAGGCAGTATGCTGCCGGTTACGTCATTTTCTGCGTTTTCGCCTTTCCGAAATCTGAGTGc                            <  3:118736/136‑1 (MQ=255)
                                                                                                         ccAAAGGCAATTTTGTAATTCATCACGCGTACGCGTCCAAAAGGAAGCATTCAACCTATTGCGTCGAGGCACGAACAGGCAGTATGCTGCCGGTTACGTCATTTTCTGCGTTTTCGCCTTTCCGAAATCTGAGTGc                            <  1:277542/136‑1 (MQ=255)
                                                                                                         ccAAAGGCAATTTTGTAATTCATCACGCGTACGCGTCCAAAAGGAAGCATTCAACCTATTGCGTCGAGGCACGAACAGGCAGTATGCTGCCGGTTACGTCATTTTCTGCGTTTTCGCCTTTCCGAAATCTGAGTGc                            <  7:64426/136‑1 (MQ=255)
                                                                                                         ccAAAGGCAATTTTGTAATTCATCACGCGTACGCGTCCAAAAGGAAGCATTCAACCTATTGCGTCGAGGCACGAACAGGCAGTATGCTGCCGGTTACGTCATTTTCTGCGTTTTCGCCTTTCCGAAATCTGAGTGc                            <  7:182940/136‑1 (MQ=255)
                                                                                                         ccAAAGGCAATTTTGTAATTCATCACGCGTACGCGTCCAAAAGGAAGCATTCAACCTATTGCGTCGAGGCACGAACAGGCAGTATGCTGCCGGTTACGTCATTTTCTGCGTTTTCGCCTTTCCGAAATCTGAGTGc                            <  7:64220/136‑1 (MQ=255)
                                                                                                         ccAAAGGCAATTTTGTAATTCATCACGCGTACGCGTCCAAAAGGAAGCATTCAACCTATTGCGTCGAGGCACGAACAGGCAGTATGCTGCCGGCTACGTCATTTTCTGCGTTTTCGCCTTTCCGAAATCTGAGTGc                            <  1:218640/136‑1 (MQ=255)
                                                                                                          cAAAGGCAATTTTGTAATTCATCACGCGTACGCGTCCAAAAGGAAGCATTCAACCTATTGCGTCGAGGCACGAACAGGCAGTATGCTGCCGGTTACGTCATTTTCTGCGTTTTCGCCTTTCCGAAATCTGAGAGCt                           >  6:176084/1‑136 (MQ=255)
                                                                                                           aaaGGCAATTTTGTAATTCATCACGCGTACGCGTCCAAAAGGAAGCATTCAACCTATTGCGTCGAGGCACGAACAGGCAGTATGCTGCCGGTTACGTCATTTTCTGCGTTTTCGCCTTTCCGAAATCTGAGTGCTc                          <  7:27151/136‑1 (MQ=255)
                                                                                                            aaGGCAATTTTGTAATTCATCACGCGTGCGCGTCCAAAAGGAAGCATTCAACCTATTGCGTCGAGGCACGAACAGGCAGTATGCTGCCGGTTACGTCATTTTCTGCGTTTTCGCCTTTCCGAAATCTGAGTGCTcc                         <  4:159806/136‑1 (MQ=255)
                                                                                                                   ttttGTAATTCATCACGCGTACGCGTCCAAAAGGAAGCATTCAACCTATTGCGTCGAGGCACGAACAGGCAGTATGCTGCCGGTTACGTCATTTTCTGCGTTTTCGCCTTTCCGAAATCTGAGTGCTCCCGCCttt                  <  7:202003/136‑1 (MQ=255)
                                                                                                                      tgcaaTTCATCACGCGTACGCGTCCAAAAGGAAGCATTCAACCTATTGCGTCGAGGCACGAACAGGCAGTATGCTGCCGGTTACGTCATTTTCTGCGTTTTCGCCTTTCCGAAATCTGAGTGCTCCCGCCTTTa                 >  2:39586/4‑134 (MQ=255)
                                                                                                                      tGTAATTCATCACGCGTACGCGTCCAAAAGGAAGCATTCAACCTATTGCGTCGAGGCACGAACAGGCAGTATGCTGCCGGTTACGTCATTTTCTGCGTTTTCGCCTTTCCGAAATCTGAGTGCTCCCGCCTTTa                 >  2:224720/1‑134 (MQ=255)
                                                                                                                      tGTAATTCATCACGCGTACGCGTCCAAAAGGAAGCATTCAACCTATTGCGTCGAGGCACGAACAGGCAGTATGCTGCCGGTTACGTCATTTTCTGCGTTTTCGCCTTTCCGAAATCTGAGTGCTCCCGCCTTTa                 >  7:101044/1‑134 (MQ=255)
                                                                                                                      tGTAATTCATCACGCGTACACGTCCAAAAGGAAGCATTCAACCTATTGCGTCGAGGCACGAACAGGCAGTATGCTGCCGGTTACGTCATTTTCTGCGTTTTCGCCTTTCCGAAATCTGAGTGCTCCCGCCtt                   >  4:150738/1‑132 (MQ=255)
                                                                                                                        tAATTCATCACGCGTGCGCGTCCAAAAGGAAGCATTCAACCTATTGCGTCGAGGCACGAACAGGCAGTATGCTGCCGGTTACGTCATTTTCTGCGTTTTCGCCTTTCCGAAATCTGAGTGCTCCCGCCTTTagaga             <  8:60298/136‑1 (MQ=255)
                                                                                                                          aTTCATCACGCGTACGCGTCCAAAAGGAAGCATTCAACCTATTGCGTCGAGGCACGAACAGGCAGTATGCTGCCGGTTACGTCATTTTCTGCGTTTTCGCCTTTCCGAAATCTGAGTGCTCCCGCCTTTagagag            <  2:140850/135‑1 (MQ=255)
                                                                                                                          aTTCATCACGCGTACGCGTCCAAAAGGAAGCATTCAACCTATTGCGTCGAGGCACGAACAGGCAGTATGCTGCCGGTTACGTCATTTTCTGCGTTTTCGCCTTTCCGAAATCTGAGTGCTCCCGCCTTTagagag            <  4:252268/135‑1 (MQ=255)
                                                                                                                           ttCATCACGCGTACGCGTCCAAAAGGAAGCATTCAACCTATTGCGTCGAGGCACGAACAGGCAGTATGCTGCCGGTTACGTCATTTTCTGCGTTTTCGCCTTTCCGAAATCTGAGTGCTCCCGCCTTTAGAGAGTc          <  3:105156/136‑1 (MQ=255)
                                                                                                                                caCGCGTACGCGTCCAAAAGGAAGCATTCAACCTATTGCGTCGAGGCACGAACAGGCAGTATGCTGCCGGTTACGTCATTTTCTGCGTTTTCGCCGTTCCGAAATCTGAGTGCTCCCGCCTTTAGAGAGTGCTAgg     >  3:198626/1‑136 (MQ=255)
                                                                                                                                  cgcgtacgcgtCCAAAAGGAAGCATTCAACCTATTGCGTCGAGGCACGAACAGGCAGTATGCTGCCGGTTACGTCATTTTCTGCGTTTTCGCCTTTCCGAAATCTGAGTGCTCCCGCCTTTAGAGAGTCCTCGGGa   <  2:158670/136‑1 (MQ=255)
                                                                                                                                   gcgtacgcgtCCAAAAGGAAGCATTCAACCTATTGCGTCGAGGCACGAACAGGCAGTATGCTGCCGGTTACGTCATTTTCTGCGTTTTCGCCTTTCCGAAATCTGAGTGCTCCCGCCTTTAGAGAGTCCTCGGGag  >  8:118795/1‑135 (MQ=255)
                                                                                                                                       |                                                                                                                                   
GAGTACTCACCTTTCTCCAAATTTCTGATGTACCTGACGCTGGCGGCAGGATCAAAAGCTTCCTGCGTAAGGCCTCGCTGAACACGCAGTTGGCGGAATACGCGGCCAAAGGCAATTTTGTAATTCATCACGCGTACGCGTCCAAAAGGAAGCATTCAACCTATTGCGTCGAGGCACGAACAGGCAGTATGCTGCCGGTTACGTCATTTTCTGCGTTTTCGCCTTTCCGAAATCTGAGTGCTCCCGCCTTTAGAGAGTCCTCGGGAA  >  ACB143/2174938‑2175204

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: