Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 2,908,411 A→G 67.9% Y1464C (TAC→TGC)  CJ019_02759 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1432,908,4110AG67.9% 69.9 / 44.7 53Y1464C (TAC→TGC) CJ019_02759hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (9/8);  new base G (15/21);  total (24/29)
Fisher's exact test for biased strand distribution p-value = 5.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.64e-01

CCTGCTACCGGCCGGCGACCTGGCCTTCCAGAGCGCTGATGGCACTTCGTTCAAGGTGTCGCCAGACCTGGCCGGGCTGAGCCTGCGGGTCAAGGCGATCTATCAGGATGCCCATGGCACCACCGAAGTGCTGTACTCGCAGCCTACCACCGTGGTGCAACCGGGCGCGCCCGTGGTGCCGACAGTGGCCACCCCGGTGGTGGATGCCAGTGACGGCGGTGCCGGCCTGCACATGGTGCGCTCCGACCTGAACTTCATCCTC  >  ACB143/2908277‑2908538
                                                                                                                                      |                                                                                                                               
ccTGCTACCGGCCGGCGACCTGGCCTTCCAGAGCGCTGATGGCACTTCGTTCAAGGTGTCGCCAGACCTGGCCGGGCTGAGCCTGCGGGTCAAGGCGATCTATCAGGATGCCCATGGCACCACCGAAGTGCTGTAc                                                                                                                                <  7:163050/136‑1 (MQ=255)
ccTGCTACCGGCCGGCGACCTGGCCTTCCAGAGCGCTGATGGCACTTCGTTCAAGGTGTCGCCAGACCTGGCCGGGCTGAGCCTGCGGGTCAAGGCGATCTATCAGGATGCCCATGGCACCACCGAAGTGCTGTAc                                                                                                                                >  6:256839/1‑136 (MQ=255)
ccTGCTACCGGCCGGCGACCTGGCCTTCCAGAGCGCTGATGGCACTTCGTTCAAGGTGTCGCCAGACCTGGCCGGGCTGAGCCTGCGGGTCAAGGCGATCTATCAGGATGCCCATGGCACCACCGAAGTGCTGTAc                                                                                                                                >  2:239581/1‑136 (MQ=255)
     tACCGGCCGGCGACCTGGCCTTCCAGAGCGCTGATGGCACTTCGTTCAAGGTGTCGCCAGACCTGGCCGGGCTGAGCCTGCGGGTCAAGGCGATCTATCAGGATGCCCATGGCACCACCGAAGTGCTGTACTCg                                                                                                                             >  8:224891/1‑134 (MQ=255)
       ccggccggCGACCTGGCCTTCCAGAGCGCTGATGGCACTTCGTTCAAGGTGTCGCCAGACCTGGCCGGGCTGAGCCTGCGGGTCAAGGCGATCTATCAGGATGCCCATGGCACCACCGAAGTGCTGTGCTCGCAGc                                                                                                                         >  4:226157/1‑136 (MQ=255)
       ccggccggCGACCTGGCCTTCCAGAGCGCTGATGGCACTTCGTTCAAGGTGTCGCCAGACCTGGCCGGGCTGAGCCTGCGGGTCAAGGCGATCTATCAGGATGCCCATGGCACCACCGAAGTGCTGTACTCGCAGc                                                                                                                         >  8:218431/1‑136 (MQ=255)
        cggccggcGACCTGGCCTTCCAGAGCGCTGATGGCACTTCGTTCAAGGTGTCGCCAGACCTGGCCGGGCTGAGCCTGCGGGTCAAGGCGATCTATCAGGATGCCCATGGCACCACCGAAGTGCTGTGCTCGCAGcc                                                                                                                        <  3:95644/136‑1 (MQ=255)
          gccggcGACCTGGCCTTCCAGAGCGCTGATGGCACTTCGTTCAAGGTGTCGCCAGACCTGGCCGGGCTGAGCCTGCGGGTCAAGGCGATCTATCAGGATGCCCATGGCACCACCGAAGTGCTGTGCTCGCAGCCTa                                                                                                                      >  1:177651/1‑136 (MQ=255)
            cggcGACCTGGCCTTCCAGAGCGCTGATGGCACTTCGTTCAAGGTGTCGCCAGACCTGGCCGGGCTGAGCCTGCGGGTCAAGGCGATCTATCAGGATGCCCATGGCACCACCGAAGTGCTGTGCTCGCAGCCTac                                                                                                                     >  6:228776/1‑135 (MQ=255)
            cggcGACCTGGCCTTCCAGAGCGCTGATGGCACTTCGTTCAAGGTGTCGCCAGACCTGGCCGGGCTGAGCCTGCGGGTCAAGGCGATCTATCAGGATGCCCATGGCACCACCGAAGTGCTGTGCTCGCAGCCTac                                                                                                                     >  6:224285/1‑135 (MQ=255)
             ggcGACCTGTCCTTCCAGAGCGCTGATGGCTCTTCGTTCAAGGTGTCGCCAGACCTGGCCGGGCTGAGCCTGCGGGTCAAGGCGATCTATCAGGATGCCCATGGCACCACCGAAGTGCTGTGCTCGCAGCCTacca                                                                                                                   <  8:98727/136‑1 (MQ=255)
             ggcGACCTGGCCTTCCAGAGCGCTGATGGCACTTCGTTCAAGGTGTCGCCAGACCTGGCCGGGCTGAGCCTGCGGGTCAAGGCGATCTATCAGGATGCCCATGGCACCACCGAAGTGCTGTGCTCGCAGCCTacca                                                                                                                   >  4:298264/1‑136 (MQ=255)
             ggcGACCTGGCCTTCCAGAGCGCTGATGGCACTTCGTTCAAGGTGTCGCCAGACCTGGCCGGGCTGAGCCTGCGGGTCAAGGCGATCTATCAGGATGCCCATGGCACCACCGAAGTGCTGTGCTCGCAGCCTacca                                                                                                                   <  7:7083/136‑1 (MQ=255)
              gcGACCTGGCCTTCCAGAGCGCTGATGGCACTTCGTTCAAGGTGTCGCCAGACCTGGCCGGGCTGAGCCTGCGGGTCAAGGCGATCTAACAGGATGCCCATGGCACCACCGAAGTGCTGTGCTCGCAGCCTaccac                                                                                                                  <  5:77357/136‑1 (MQ=255)
              gcGACCTGGCCTTCCAGAGCGCTGATGGCACTTCGTTCAAGGTGTCGCCAGACCTGGCCGGGCTGAGCCTGCGGGGCAAGGCGATCTATCAGGATGCCCATGGCACCACCGAAGTGCTGTGCTCGCAGCCTaccac                                                                                                                  <  5:278115/136‑1 (MQ=255)
                    tGGCCTTCCAGAGCGCTGATGGCACTTCGTTCAAGGTGTCGCCAGACCTGGCCGGGCTGAGCCTGCGGGTCAAGGCGATCTATCAGGATGCCCATGGCACCACCGAAGTGCTGTGCTCGCAGCCTACCACCgtggt                                                                                                            >  5:3387/1‑136 (MQ=255)
                           ccAGAGCGCTGATGGCACTTCGTTCAAGGTGTCGCCAGACCTGGCCGGGCTGAGCCTGCGGGGCAAGGCGATCTATCAGGATGCCCATGGCACCACCGAAGTGCTGTGCTCGCAGCCTACCACCGTGGTGCAAcc                                                                                                      <  2:249933/135‑1 (MQ=255)
                             agagCGCTGATGGCACTTCGTTCAAGGTGTCGCCAGACCTGGCCGGGCTGAGCCTGCGGGTCAAGGCGATCTATCAGGATGCCCATGGCACCACCGAAGTGCTGTACTCGCAGCCTACCACCGTGGTGCAACCggg                                                                                                   <  6:93733/136‑1 (MQ=255)
                              gagCGCTGATGGCACTTCGTTCAAGGTGTCGCCAGACCTGGCCGGGCTGAGCCTGCGGGTCAAGGCGATCTATCAGGATGCCCATGGCACCACCGAAGTGCTGTACTCGCAGCCTACCACCGTGGTGCAACCGGgc                                                                                                  >  4:238771/1‑136 (MQ=255)
                               agCGCTGATGGCACTTCGTTCAAGGTGTCGCCAGACCTGGCCGGGCTGAGCCTGCGGGTCAAGGCGATCTATCAGGATGCCCATGGCACCACCGAAGTGCTGTGCTCGCAGCCTACCACCGTGGTGCAACCGGgcg                                                                                                 <  3:289301/136‑1 (MQ=255)
                                 cgcTGATGGCACTTCGTTCAAGGTGTCGCCAGACCTGGCCGGGCTGAGCCTGCGGGTCAAGGCGATCTATCAGGATGCCCATGGCACCACCGAAGTGCTGTGCTCGCAGCCTACCACCGTGGTGCAACCGGgcgcg                                                                                               <  1:66491/136‑1 (MQ=255)
                                 cgcTGATGGCACTTCGTTCAAGGTGTCGCCAGACCTGGCCGGGCTGAGCCTGCGGGTCAAGGCGATCTATCAGGATGCCCATGGCACCACCGAAGTGCTGTACTCGCAGCCTACCACCGTGGTGCAACCGGgcgcg                                                                                               <  8:225729/136‑1 (MQ=255)
                                 cgcTGATGGCACTTCGTTCAAGGTGTCGCCAGACCTGGCCGGGCTGAGCCTGCGGGTCAAGGCGATCTATCAGGATGCCCATGGCACCACCGAAGTGCTGTACTCGCAGCCTACCACCGTGGTGCAACCGGgcgcg                                                                                               <  6:188156/136‑1 (MQ=255)
                                    tGATGGCAATTCGTTCAAGGTGTCGCCAGACCTGGCCGGGCTGACCCTGCGGGTCAACGCGATCTATCAGCATGCCCATGGCCCCACCGAAGTGCTGTGCTCGCAGCCTACCACCGTGGTGCAAGCGGGCGCGccc                                                                                            >  2:298048/1‑136 (MQ=255)
                                               agTTCAAGGTGTCGCCATACCTGGCCGGGCTGAGCCTGCGGGTCAAGGCGATCTATCAGGATGCCCATGGCACCACCGAAGTGCTGTGCGCGCAGCCTACCACCGTGGTGCAACCGGGCGCGCCCGGGGTGCCg                                                                                   <  1:311652/133‑1 (MQ=255)
                                                 ttCAAGGTGTCGCCAGACCTGGCCGGGCTGAGCCTGCGGGTCAAGGCGATCTATCAGGATGCCCATGGCACCACCGAAGTGCTGTACTCGCAGCCTACCACCGTGGTGCAACCGGGCGCGCCCGTGGTGCCGACAg                                                                               >  3:209250/1‑136 (MQ=255)
                                                   cAAGGTGTCGCCAGACCTGGCCGGGCTGAGCCTGCGGGTCAAGGCGATCTATCAGGATGCCCATGGCACCACCGAAGTGCTGTGCTCGCAGCCTACCACCGTGGTGCAACCGGGCGCGCCCGTGGTGCCGACAGt                                                                              <  4:146149/135‑1 (MQ=255)
                                                          tCGCCAGACCTGGCCGGGCTGAGCCTGCGGGTCAAGGCGATCTATCAGGATGCCCATGGCACCACCGAAGTGCTGTGCTCGCAGCCTACCACCGGGGTGCAACCGGGCGCGCCCGTGGTGCCGACAGTGGCCAccc                                                                      <  7:57960/136‑1 (MQ=255)
                                                          tCGCCAGACCTGGCCGGGCTGAGCCTGCGGGTCAAGGCGATCTATCAGGATGCCCATGGCACCACCGAAGTGCTGTACTCGCAGCCTACCACCGTGGTGCAACCGGGCGCGCCCGTGGTGCCGACAGTGGCCAccc                                                                      <  1:306691/136‑1 (MQ=255)
                                                          gcGCCAGACCTGGCCGGGCTGAGCCTGCGGGTCAAGGCGATCTATCAGGATGCCCATGGCACCACCGAAGTGCTGTACTCGCAGCCTACCACCGTGGTGCAACCGGGCGCGCCCGTGGTGCCGACAGTGGCCAccc                                                                      <  5:79242/135‑1 (MQ=255)
                                                             ccAGACCTGGCCGGGCTGAGCCTGCGGGTCAAGGCGATCTATCAGGATGCCCATGGCACCACCGAAGTGCTGTGCTCGCAGCCTACCACCGTGGTGCACCCGGGCGCGCCCGTGGTGCCGACAGTGGCCACCCCgg                                                                   >  8:293965/1‑136 (MQ=255)
                                                             ccAGACCTGGCCGGGCTGAGCCTGCGGGTCAAGGCGATCTATCAGGATGCCCATGGCACCACCGAAGTGCTGTACTCGCAGCCTACCACCGTGGTGCAACCGGGCGCGCCCGTGGTGCCGACAGTGGCCACCCCgg                                                                   >  3:171316/1‑136 (MQ=255)
                                                              cAGACCTGGCCGGGCTGAGCCTGCGGGTCAAGGCGATCTATCAGGATGCCCATGGCACCACCGAAGTGCTGTGCTCGCAGCCTACCACCGTGGTGCAACCGGGCGCGCCCGTGGTGCCGACAGTGGCCACCCCggt                                                                  >  3:126345/1‑136 (MQ=255)
                                                                        cGGGCTGAGCCTGCGGGTCAAGGCGATCTATCAGGATGCCCATGGCACCACCGAAGTGCTGTGCTCGCAGCCTACCACCGTGGTGCAACCGGGCGCGCCCGTGGTGCCGACAGTGGCCACCCCGGTGGTGGATGcc                                                        <  2:41005/136‑1 (MQ=255)
                                                                         gggCTGAGCCTGCGGGTCAAGGCGATCTATCAGGATGCCCATGGCACCACCGAAGTGCTGTGCTCGCAGCCTACCACCGTGGTGCAACCGGGCGCGCCCGTGGTGCCGACAGTGGCCACCCCGGTGGTGGATGc                                                         >  4:264328/1‑134 (MQ=255)
                                                                         gggCTGAGCCTGCGGGTCAAGGCGATCTATCAGGATGCCCATGGCACCACCGAAGTGCTGTACTCGCAGCCTACCACCGTGGTGCAACCGGGCGCGCCCGTGGTGCCGCCAGTGGCCACCCCGGTGGTGGATGc                                                         >  3:84203/1‑134 (MQ=255)
                                                                                  cTGCGGGTCAAGGCGATCTATCAGGATGCCCATGGCACCACCGAAGTGCTGTGCTCGCAGCCTACCACCGTGGTGCAACCGGGCGCGCCCGTGGTGCCGACAGTGGCCACCCCGGTGGTGGATGCCAGTGAcggcg                                              <  2:188217/136‑1 (MQ=255)
                                                                                  cTGCGGGTCAAGGCGATCTATCAGGATGCCCATGGCACCACCGAAGTGCTGTACTCGCAGCCTACCACCGTGGTGCAACCGGGCGCGCCCGTGGTGCCGACAGTGGCCACCCCGGTGGTGGATGCCAGTGAcggcg                                              <  7:224891/136‑1 (MQ=255)
                                                                                  cTGCGGGTCAAGGCGATCTATCAGGATGCCCATGGCACCACCGAAGTGCTGTACTCGCAGCCTACCACCGTGGTGCAACCGGGCGCGCCCGTGGGGCCGACATTGGCCACCCCGGTGGTGGATGCCAGTGAcggcg                                              <  7:181033/136‑1 (MQ=255)
                                                                                    gCGGGTCAAGGCGATCTATCAGGATGCCCATGGCACCACCGAAGTGCTGTGCTCGCAGCCTACCACCGTGGTGCAACCGGGCGCGCCCGTGGTGCCGACAGTGGCCACCCCGGTGGTGGATGCCAGTGACGGCGGt                                            <  1:162737/136‑1 (MQ=255)
                                                                                     cGGGTCAAGGCGATCTATCAGGATGCCCATGGCACCACCGAAGTGCTGTGCTCGCAGCCTACCACCGTGGTGCAACCGGGCGCGCCCGTGGTGCCGACAGTGGCCACCCCGGTGGTGGATGCCAGTGAcggcgg                                             <  3:121343/134‑1 (MQ=255)
                                                                                        gTCAAGGCGATCTATCAGGATGCCCATGGCACCACCGAAGTGCTGTGCTCGCAGCCTACCACCGTGGTGCAACCGGGCGCGCCCGTGGTGCCGACAGTGGCCACCCCGGTGGTGGATGCCAGTGACGGCGGTGCCg                                        >  8:215838/1‑136 (MQ=255)
                                                                                        gTCAAGGCGATCTATCAGGATGCCCATGGCACCACCGAAGTGCTGTGCTCGCAGCCTACCACCGTGGTGCAACCGGGCGCGCCCGTGGTGCCGACAGTGGCCACCCCGGTGGTGGATGCCAGTGACGGCGGTGCCg                                        >  2:36313/1‑136 (MQ=255)
                                                                                        gTCAAGGCGATCTATCAGGATGCCCATGGCACCACCGAAGTGCGGTGCTCGCAGCCTACCACCGTGGTGCAACCGGGCGCGCCCGTGGTGCCTACAGTGGCCACCCCGGTGGTGGATGCCAGTGACGGCGGTGCCg                                        <  2:316945/136‑1 (MQ=255)
                                                                                              gCGATCTATCAGGATGCCCATGGCACCACCGAAGTGCTGTGCTCGCAGCCTACCACCGTGGTGCAACCGGGCGCGCCCGTGGTGCCGACAGTGGCCACCCCGGTGGTGGATGCCAGTGACGGCGGTGCCGGCCTg                                   >  4:228194/1‑135 (MQ=255)
                                                                                              gCGATCTATCAGGATGCCCATGGCACCACCGAAGTGCTGTACTCGCAGCCTACCACCGTGGTGCAACCGGGCGCGCCCGTGGTGCCGACAGTGGCCACCCCGGTGGTGGATGCCAGTGACGGCGGTGCCGGCCTGc                                  >  6:123214/1‑136 (MQ=255)
                                                                                                        aGGATGCCCATGGCACCACCGAAGTGCTGTGCTCGCAGCCTACCACCGTGGTGCAACCGGGCGCGCCCGTGGTGCCGACAGTGGCCACCCCGGTGGTGGATGCCAGTGACGGCGGTGCCGGCCTGCACATGGTgc                         <  7:249285/135‑1 (MQ=255)
                                                                                                         ggATGCCCATGGCACCACCTAAGGGCTGTGCTCGCAGCCTACCCCCGTGGTGCAACCGGGCGCGCCCGTGGTGCTGACAGTGGCCACCCCGGTGGTGGATGCCAGTGACGGCGGTGCCGGCCTGCACATGGTgcgc                       <  3:217126/136‑1 (MQ=255)
                                                                                                         ggATGCCCATGGCACCACCGAAGTGCTGTGCTCGCAGCCTACCACCGTGGTGCAACCGGGCGCGCCCGTGGTGCCGACAGTGGCCACCCCGGTGGTGGATGCCAGTGACGGCGGTGCCGGCCTGCACATGGTgcgc                       <  3:142606/136‑1 (MQ=255)
                                                                                                               ccATGGCACCACCGAAGTGCTGTGCTCGCAGCCTACCACCGTGGTGCAACCGGGCGCGCCCGTGGTGCCGACAGTGGCCACCCCGGTGGTGGATGCCAGTGACGGCGGTGCCGGCCTGCACATGGTGCGCTCCGAc                 <  1:310357/136‑1 (MQ=255)
                                                                                                                 aTGGCACCACCGAAGTGCTGTGCTCGCAGCCTACCACCGTGGTGCAACCGGGCGCGCCCGTGGTGCCGACAGTGGCCACCCCGGTGGTGGATGCCAGTGACGGCGGTGCCGGCCTGCACATGGTGCGCTCCGACCt               >  3:158548/1‑136 (MQ=255)
                                                                                                                        caccGAAGTGCTGTGCTCGCAGCCTACCACCGTGGTGCAACCGGGCGCGCCCGTGGTGCCGACAGTGGCCACCCCGGTGGTGGATGCCAGTGACGGCGGTGCCGGCCTGCACATGGTGCGCTCCGACCTGAACtt         >  4:272184/1‑135 (MQ=255)
                                                                                                                              aGTGCTGTGCTCGCAGCCTACCACCGTGGTGCAACCGGGCGCGCCCGTGGTGCCGACAGTGGCCACCCCGGTGGTGGATGCCAGTGACGGCGGTGCCGGCCTGCACATGGTGCGCTCCGACCTGAACTTCATCCTc  <  6:28303/136‑1 (MQ=255)
                                                                                                                                      |                                                                                                                               
CCTGCTACCGGCCGGCGACCTGGCCTTCCAGAGCGCTGATGGCACTTCGTTCAAGGTGTCGCCAGACCTGGCCGGGCTGAGCCTGCGGGTCAAGGCGATCTATCAGGATGCCCATGGCACCACCGAAGTGCTGTACTCGCAGCCTACCACCGTGGTGCAACCGGGCGCGCCCGTGGTGCCGACAGTGGCCACCCCGGTGGTGGATGCCAGTGACGGCGGTGCCGGCCTGCACATGGTGCGCTCCGACCTGAACTTCATCCTC  >  ACB143/2908277‑2908538

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑