| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB143 | 4,003,911 | G→A | 28.6% | R113C (CGC→TGC) | pobA ← | p‑hydroxybenzoate hydroxylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB143 | 4,003,911 | 0 | G | A | 28.6% | 52.2 / 23.9 | 35 | R113C (CGC→TGC) | pobA | p‑hydroxybenzoate hydroxylase |
| Reads supporting (aligned to +/- strand): ref base G (11/14); new base A (5/5); total (16/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01 | |||||||||||
AGCCGGCGATATAGTCGCAGTCAATCCGCTGCACCCGGCCATCCTTTTCGAACGTCAGGTAGGGCTTCTCGCCTTTCAATTCATGCGGCTGAACGTTGGCGGCTGAATAAATGATCGGCGCACCACTGGCTTCGCGGGCCTGCATCAGGTCACGGGTGACTTCGGTCTGGCCGTAGACCATCACCGTCTTGCCGCCGGTCAGGGCTTTGAGATCCAGACGCTGGCGGCGCCCGCCAACCAGCAGTTCAACCCCCTCGTGCACCAGGCCTT > ACB143/4003776‑4004045 | aGCCGGCGATATAGTCGCAGTCAATCCGCTGCACCCGGCCATCCTTTTCGAACGTCAGGTAGGGCTTCTCGCCTTTCAATTCATGCGGCTGAACGTTGGCGGCTGAATAAATGATCGGCGCACCACTGGCTTcgcg < 7:88341/136‑1 (MQ=255)aGCCGGCGATATAGTCGCAGTCAATCCGCTGCACCCGGCCATCCTTTTCGAACGTCAGGTAGGGCTTCTCGCCTTTCAATTCATGCGGCTGAACGTTGGCGGCTGAATAAATGATCGGCGCACCACTGGCTTcgcg < 6:248740/136‑1 (MQ=255) cGATATAGTCGCAGTCAATCCGCTGCACCCGGCCATCCTTTTCGAACGTCAGGTAGGGCTTCTCGCCTTTCAATTCATGCGGCTGAACGTTGGCGGCTGAATAAATGATCGGCGCACCACTGGCTTCGCGGGCCTg < 1:146070/136‑1 (MQ=255) cGATATAGTCGCAGTCAATCCGCTGCACCCGGCCATCCTTTTCGAACGTCAGGTAGGGCTTCTCGCCTTTCAATTCATGCGGCTGAACGTTGGCGGCTGAATAAATGATCGGCGCACCACTGGCTTCGCAGGCCTg < 6:255800/136‑1 (MQ=255) cGATATAGTCGCAGTCAATCCGCTGCACCCGGCCATCCTTTTCGAACGTCAGGTAGGGCTTCTCGCCTTTCAATTCATGCGGCTGAACGTTGGCGGCTGAATAAATGATCGGCGCACCACTGGCTTCGCAGGCCTg < 3:18888/136‑1 (MQ=255) cAGTCAATCCGCTGCACCCGGCCATCCTTTTCGAACGTCAGGTAGGGCTTCTCGCCTTTCAATTCATGCGGCTGAACGTTGGCGGCTGAATAAATGATCGGCGCACCACTGGCTTCGCGGGCCTGCATCAGGTCAc > 5:311631/1‑136 (MQ=255) gTCAATCCGCTGCACCCGGCCATCCTTTTCGAACGTCAGGTAGGGCTTCTCGCCTTTCAATTCATGCGGCTGAACGTTGGCGGCTGAATAAATGATCGGCGCACCACTGGCTTCGCGGGCCTg > 1:74200/1‑123 (MQ=255) gTCAATCCGCTGCACCCGGCCATCCTTTTCGAACGTCAGGTAGGGCTTCTCGCCTTTCAATTCATGCGGCTGAACGTTGGCGGCTGAATAAATGATCGGCGCACCACTGGCTTCGCGGGCCTg < 2:74200/123‑1 (MQ=255) aaTCCGCTGCACCCGGCCATCCTTTTCGAACGTCAGGTAGGGCTTCTCGCCTTTCAATTCATGCGGCTGAACGTTGGCGGCTGAATAAATGATCGGCGCACCCCTGGCTTCGCAGGCCTGCATCAGGTCCCGGGTg > 7:273073/1‑136 (MQ=255) gCTGCACCCGGCCATCCTTTTCGAACGTCAGGTAGGGCTTCTCGCCTTTCAATTCATGCGGCTGAACGTTGGCGGCTGAATAAATGATCGGCGCACCACTGGCTTCGCGGGCCTGCATCAGGTCACGGGTGACTTc < 7:294607/136‑1 (MQ=255) gCTGCACCCGGCCATCCTTTTCGAACGTCAGGTAGGGCTTCTCGCCTTTCAATTCATGCGGCTGAACGTTGGCGGCTGAATAAATGATCGGCGCACCACTGGCTTCGCGGGCCTGCATCAGGTCACGGGTGACTTc < 4:186950/136‑1 (MQ=255) cTGCACCCGGCCATCCTTTTCGAACGTCAGGTAGGGCTTCTCGCCTTTCAATTCATGCGGCTGAACGTTGGCGGCTGAATAAATGATCGGCGCACCACTGGCTTCGCGGGCCTGCATCAGGTCACGGGTGACTTCg < 8:65836/136‑1 (MQ=255) cACCCGGCCATCCTTTTCGAACGTCAGGTAGGGCTTCTCGCCTTTCAATTCATGCGGCTGAACGTTGGCGGCTGAATAAATGATCGGCGCCCCACTGGCTTCGCGGGCCTGCATCAGGTCACGGGTGACTTCGGTc > 7:151137/1‑136 (MQ=255) cACCCGGCCATCCTTTTCGAACGTCAGGTAGGGCTTCTCGCCTTTCAATTCATGCGGCTGAACGTTGGCGGCTGAATAAATGATCGGCGCACCACTGGCTTCGCGGGCCTGCATCAGGTCACGGGTGACTTCGGTc > 2:206752/1‑136 (MQ=255) cccGGCCATCCTTTTCGAACGTCAGGTAGGGCTTCTCGCCTTTCAATTCATGCGGCTGAACGTTGGCGGCTGAATAAATGATCGGCGCACCACTGGCTTCGCAGGCCTGCATCAGGTCACGGGTGACTTCGGTCTg > 2:29782/1‑136 (MQ=255) ccGGCCATCCTTTTCGAACGTCAGGTAGGGCTTCTCGCCTTTCAATTCATGCGGCTGAACGTTGGCGGCTGAATAAATGATCGGCGCACCACTGGCTTCGCGGGCCTGCATCAGGTCATGGGTGACTTCGGTCTgg < 3:263987/136‑1 (MQ=255) cGGCCATCCTTTTCGAACGTCAGGTAGGGCTTCTCGCCTTTCAATTCATGCGGCTGAACGTTGGCGGCTGAATAAATGATCGGCGCACCACTGGCTTCGCAGGCCTGCATCAGTTCACGGGTGACTTCGGTCTGGc > 2:229358/1‑136 (MQ=255) tctcGCCTTTCAATTCATGCGGCTGAACGTTGGCGGCTGAATAAATGATCGGCGCACCACTGGCTTCGCGGGCCTGCATCAGGTCACGGGTGACTTCGGTCTGGCCGTAGACCATCACCGTCTTGCCGCCGGTCAg > 8:101637/1‑136 (MQ=255) gCCTTTCAATTCATGCGGCTGAACGTTGGCGGCTGAATAAATGATCGGCGCACCACTGGCTTCGCGGGCCTGCATCAGGTCACGGGTGACTTCGGTCTGGCCGTAGACCATCACCGTCTTGCCGCCGGTCAGGGCt > 7:303898/1‑136 (MQ=255) gCCTTTCAATTCATGCGGCTGAACGTTGGCGGCTGAATAAATGATCGGCGCACCACTGGCTTCGCGGGCCTGCATCAGGTCACGGGTGACTTCGGTCTGGCCGTAGACCATCACCGTCTTGCCGCCGGTCAGGGCt > 7:114656/1‑136 (MQ=255) cTTTCAATTCATGCGGCTGAACGTTGGCGGCTGAATAAATGATCGGAGCACCACTGGCTTCGCGGGCCTGCATCAGGTCACGGGTGACTTCGCTCTGGACGTAGACAATCACAGTCTTGCCGCCGGTCAGGGCttt > 6:209479/1‑136 (MQ=255) tCAATTCATGCGGCTGAACGTTGGCGGCTGAATAAATGATCGGCGCACCACTGGCTTCGCGGGCCTGCATCAGGTCACGGGTGACTTCGGTCTGGCCGTAGACCATCACCGTCTTGCCGCCGGTCAGGGCttt > 2:221894/1‑133 (MQ=255) gCTGAACGTTGGCGGCTGAATAAATGATCGGCGCACCACTGGCTTCGCAGGCCTGCATCAGGTCACGGGTGACTTCGGTCTGGCCGTAGACCATCACCGTCTTGCCGCCGGTCAGGGCTTTGAGATCCAGACGCTg > 1:222201/1‑136 (MQ=255) gCTGAACGTTGGCGGCTGAATAAATGATCGGCGCACCACTGGCTTCGCAGGCCTGCATCAGGTCACGGGTGACTTCGGTCTGGCCGTAGACCATCACCGTCTTGCCGCCGGTCAGGGCTTTGAGATCCAGACGCTg > 8:198685/1‑136 (MQ=255) aaCGTTGGCGGCTGAATAAATGATCGGCGCACCACTGGCTTCGCAGGCCTGCATCAGGTCACGGGTGACTTCGGTCTGGCCGTAGACCATCACCGTCTTGCCGCCGGTCAGGGCTTTGAGATCCAGACGCTggc < 8:220422/134‑1 (MQ=255) gTTGGCGGCTGAATAAATGATCGGCGCACCACTGGCTTCGCGGGCCTGCATCAGGTCACGGGTGACTTCGGTCTGGCCGTAGACCATCACCGTCTTGCCGCCGGTCAGGGCGTTGAGATCCAGACGCTGGCGgcgc < 8:262019/136‑1 (MQ=255) ggcggcTGAATAAATGCTCGGCGCACCACTGGCTTCGCAGGCCTGCATCAGGTCACGGGTGACTTCGGTCTGGCCGTAGACCATCACCGTCTTGCCGCCGGTCAGGGCTTTGAGATCCAGACGCTGGCGGcgccc < 4:91772/135‑1 (MQ=255) gcTGAATAAATGATCGGCGCACCACTGGCTTCGCGGGCCTg < 7:295909/41‑1 (MQ=255) gcTGAATAAATGATCGGCGCACCACTGGCTTCGCGGGCCTg > 8:295909/1‑41 (MQ=255) aaTAAATGATCGGCGCACCACTGGCTTCGCGGGCCTGCATCAGGTCACGGGTGACTTCGGTCTGGCCGTAGACCATCACCGTCTTGCCGCCGGTCAGGGCTTTGAGATCCAGACGCTGGCGGCGCCCGCCAAcc < 7:172962/134‑1 (MQ=255) tAAATGATCGGCGCACCACTGGCTTCGCGGGCCTGCATCAGGTCACGGGTGACTTCGGTCTGGCCGTAGACCATCACCGTCTTGCCGCCGGTCAGGGCTTTGAGATCCAGACGCTGGCGGCGCCCGCCAACcagca < 6:72696/136‑1 (MQ=255) aTCGGCGCACCACTGGCTTCGCGGGCCTGCATCAGGTCACGGGTGACTTCGGTCTGGCCGTAGACCATCACCGTCTTGCCGCCGGTCAGGGCTTTGAGATCCAGACGCTGGCGGCGCCCGCCAACCAGCAGTTCa < 6:311631/135‑1 (MQ=255) ggCGCACCACTGGCTTCGCGGGCCTGCATCAGGTCACGGGTGACTTCGGTCTGGCCGTAGACCATCACCGTCTTGCCGCCGGTCAGGGCTTTGAGATCCAGACGCTGGCGGCGCCCGCCAACCAGCAGTTCAAccc < 4:16603/136‑1 (MQ=255) cgcACCACTGGCTTCGCGGGCCTGCATCAGGTCACGGGTGACTTCGGTCTGGCCGTAGCCCATCCCCGTCTTGCCGCCGGTCAGGGCTTTGAGATCCAGACGCTGGCGGCGCCCGCCCCCCAGCAGGTCAAccccc > 7:59832/1‑136 (MQ=255) cacTGGCTTCGCGGGCCTGCATCAGGTCACGGGTGACTTCGGTCTGGCCGTAGACCATCACCGTCTTGCCGCCGGTCAGGGCTTTGAGATCCAGACGCTGGCGGCGCCCGCCAACCAGCAGTTCAACCCCCTCGTg < 1:90540/136‑1 (MQ=255) cTGGCTTCGCGGGCCTGCATCAGGTCACGGGTGACTTCGGTCTGGCCGTAGACCATCACCGTCTTGCCGCCGGTCAGGGCTTTGAGATCCAGACGCTGGCGGCGCCCGCCAACCAGCAGTTCAACCCCCTCGTGCa < 1:44628/136‑1 (MQ=255) cTGGCTTCGCAGGCCTGCATCAGGTCACGGGTGACTTCGGTCTGGCCGTAGACCATCACCGTCTTGCCGCCGGTCAGGGCTTTGAGATCCAGACGCTGGCGGCGCCCGCCAACCAGCAGTTCAACCCCCTCGTGCa < 1:29782/136‑1 (MQ=255) gcgGGCCTGCATCAGGTCACGGGTGACTTCGGTCTGGCCGTAGACCATCACCGTCTTGCCGCCGGTCAGGGCTTTGAGATCCAGACGCTGGCGGCGCCCGCCAACCAGCAGTTCAACCCCCTCGTGCACCAGGCCt < 7:101637/136‑1 (MQ=255) cgGGCCTGCATCAGGTCACGGGTGACTTCGGGCTGGCCGTAGACCATCACCGTCTTGCCGCCGGTCAGGGCTTTGAGATCCAGACGCTGGCGGGGCCCGCGAACCAGCAGTTCAACCCCCTCGTGCACCAGGCGtt < 6:33997/136‑1 (MQ=255) | AGCCGGCGATATAGTCGCAGTCAATCCGCTGCACCCGGCCATCCTTTTCGAACGTCAGGTAGGGCTTCTCGCCTTTCAATTCATGCGGCTGAACGTTGGCGGCTGAATAAATGATCGGCGCACCACTGGCTTCGCGGGCCTGCATCAGGTCACGGGTGACTTCGGTCTGGCCGTAGACCATCACCGTCTTGCCGCCGGTCAGGGCTTTGAGATCCAGACGCTGGCGGCGCCCGCCAACCAGCAGTTCAACCCCCTCGTGCACCAGGCCTT > ACB143/4003776‑4004045 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |