Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 4,669,221 C→T 7.8% G318R (GGG→AGG)  adiA ← Biodegradative arginine decarboxylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1434,669,2210CT 7.8% 163.1 / 4.0 51G318R (GGG→AGG) adiABiodegradative arginine decarboxylase
Reads supporting (aligned to +/- strand):  ref base C (23/24);  new base T (2/2);  total (25/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

CATAGGCAAACCAGGCCTCGTCGAAGTGCAGCACCTCGACACTGGCACCCAGGGCCTGCTTGATCAGCCCGGCGTGGTAACACAGCCCGTCGTAGGTGGAGTTGGTCACTACCGCCAGCTTGATACGCTGCCCACGATCACGGGCAAGGGGGTTGGCCTGAATCTTCGCGGCGATCGCCTCGGGGCTGAACTCGCTGAGCGGGATCGGGCCAATGATGCCCAGTTCGTTGCGCTCCGGGCACAGGTACAACGGGATGGCACCCGTCA  >  ACB143/4669089‑4669355
                                                                                                                                    |                                                                                                                                      
cATAGGCAAACCAGGCCTCGTCGAAGTGCAGCACCTCGACACTGGCACCCAGGGCCTGCTTGATCAGCCCGGCGTGGTAACACAGCCCGTCGTAGGTGGAGTTGGTCACTACCGCCAGCTTGATACGCTGCCCAc                                                                                                                                      >  8:38105/1‑135 (MQ=255)
cATAGGCAAACCAGGCCTCGTCGAAGTGCAGCACCTCGACACTGGCACCCAGGGCCTGCTTGATCAGCCCGGCGTGGTAACACAGCCCGTCGTAGGTGGAGTTGGTCACTACCGCCAGCTTGATACGCTGCCCAc                                                                                                                                      >  1:226662/1‑135 (MQ=255)
cATAGGCAAACCAGGCCTCGTCGAAGTGCAGCACCTCGACACTGGCACCCAGGGCCTGCTTGATCAGCCCGGCGTGGTAACACAGCCCGTCGTAGGTGGAGTTGGTCACTACCGCCAGCTTGATACGCTGCCCACg                                                                                                                                     <  3:127840/136‑1 (MQ=255)
  tAGGCAAACCAGGCCTCGTCGAAGTGCAGCACCTCGACACTGGCACCCAGGGCCTGCTTGATCAGCCCGGCGTGGTAACACAGCCCGTCGTAGGTGGAGTTGGTCACTACCGCCAGCTTGATACGCTGCCCACGAt                                                                                                                                   <  5:64858/136‑1 (MQ=255)
     gCAAACCAGGCCTCGTCGAAGTGCAGCACCTCGACACTGGCACCCAGGGCCTGCTTGATCAGCCCGGCGTGGTAACACAGCCCGTCGTAGGTGGAGTTGGTCACTACCGCCAGCTTGATACGCTGCCCACGATCAc                                                                                                                                <  4:96491/136‑1 (MQ=255)
      cAAACCAGGCCTCGTCGAAGTGCAGCACCTCGACACTGGCACCCAGGGCCTGCTTGATCAGCCCGGCGTGGTAACACAGCCCGTCGTAGGTGGAGTTGGTCACTACCGCCAGCTTGATACGCTGCCCACGATCACg                                                                                                                               >  8:249874/1‑136 (MQ=255)
              gCCTCGTCGAAGTGCAGCACCTCGACACTGGCACCCAGGGCCTGCTTGATCAGCCCGGCGTGGTAACACAGCCCGTCGTAGGTGGAGTTGGTCACTACCGCCAGCTTGATACGCTGCCCACGATCACGGGCAAggg                                                                                                                       <  5:211169/136‑1 (MQ=255)
                      gAAGTGCAGCACCTCGACACTGGCACCCAGGGCCTGCTTGATCAGCCCGGCGTGGTAACACAGCCCGTCGTAGGTGGAGTTGGTCACTACCGCCAGCTTGATACGCTGCCCACGATCACGGGCAAGGGGGTTGGc                                                                                                                <  2:56555/135‑1 (MQ=255)
                               caCCTCGACACTGGCACCCAGGGCCTGCTTGATCAGCCCGGCGTGGTAACACAGCCCGTCGTAGGTGGAGTTGGTCACTACCGCCAGCTTGATACGCTGCCCACGATCACGGGCAAGGGGGTTGGCCTGAATCTTc                                                                                                      >  3:183720/1‑136 (MQ=255)
                                     gACACTGGCACCCAGGGCCTGCTTGATCAGCCCGGCGTGGTAACACAGCCCGTCGTAGGTGGAGTTGGTCACTACCGCCAGCTTGATACGCTGCCCACGATCACGGGCAAGGGGGTTGGCCTGAATCTTCgcggc                                                                                                 <  4:104800/135‑1 (MQ=255)
                                     gACACTGGCACCCAGGGCCTGCTTGATCAGCCCGGCGTGGTAACACAGCCCGTCGTAGGTGGAGTTGGTCACTACCGCCAGCTTGATACGCTGCCCACGATCACGGGCAAGGGGGTTGGCCTGAATCTTCgcggc                                                                                                 <  3:43069/135‑1 (MQ=255)
                                       cacTGGCACCCAGGGCCTGCTTGATCAGCCCGGCGTGGTAACACAGCCCGTCGTAGGTGGAGTTGGTCACTACCGCCAGCTTGATACGCTGCCCACGCTCACGGGCAAGGGGGTTGGCCTGAATCTTCGCGGCGAt                                                                                              >  5:34604/1‑136 (MQ=255)
                                       cacTGGCACCCAGGGCCTGCTTGATCAGCCCGGCGTGGTAACACAGCCCGTCGTAGGTGGAGTTGGTCACTACCGCCAGCTTGATACGCTGCCCACGATCACGGGCAAGGGGGTTGGCCTGAATCTTCGCGGCGAt                                                                                              >  5:106678/1‑136 (MQ=255)
                                       cacTGGCACCCAGGGCCTGCTTGATCAGCCCGGCGTGGTAACACAGCCCGTCGTAGGTGGAGTTGGTCACTACCGCCAGCTTGATACGCTGCCCACGATCACGGGCAAGGGGGTTGGCCTGAATCTTCGCGGCGAt                                                                                              >  5:251584/1‑136 (MQ=255)
                                       cacTGGCACCCAGGGCCTGCTTGATCAGCCCGGCGTGGTAACACAGCCCGTCGTAGGTGGAGTTGGTCACTACCGCCAGCTTGATACGCTGCCCACGATCACGGGCAAGGGGGTTGGCCTGAATCTTCGCGGCGAt                                                                                              >  3:126717/1‑136 (MQ=255)
                                         cTGGCACCCAGGGCCTGCTTGATCAGCCCGGCGTGGTAACACAGCCCGTCGTAGGTGGAGTTGGTCACTACCGCCAGCTTGATACGCTGCCCACGATCACGGGCAAGGGGGTTGGCCTGAATCTTCGCGGCGATCg                                                                                            <  7:666/136‑1 (MQ=255)
                                         cTGGCACCCAGGGCCTGCTTGAGCAGCCCGGCGTGGTAACACAGCCCGTCGTAGGGGGAGGTGGTCACTACCGCCAGCTTGATACGCTGCCCACGATCACGGGCAAGGGGGTTGGCCTGACTCTTCGCGGCGATCg                                                                                            <  1:224744/136‑1 (MQ=255)
                                            gCACCCAGGGCCTGCTTGATCAGCCCGGCGTGGTAACACAGCCCGTCGTAGGTGGAGTTGGTCACTACCGCCAGCTTGATACGCTGCCCACGATCACGGGCAAGGGGGTTGGCCTGAATCTTCGCGGCGATCGCCt                                                                                         >  3:80922/1‑136 (MQ=255)
                                                   gggCCTGCTTGATCAGCCCGGCGTGGTAACACAGCCCGTCGTAGGTGGAGTTGGTCACTACCGCCAGCTTGATACGCTGCCCACGATCACGGGCAAGGGGGTTGGCCTGAATCTTCGCGGCGATCGCCTCGGGGCt                                                                                  <  1:63145/136‑1 (MQ=255)
                                                    ggCCTGCTTGATCAGCCCGGCGTGGTAACACAGCCCGTCGTAGGTGGAGTTGGTCACTACCGCCAGCTTGATACGCTGCCCACGATCACGGGCAAGGGGGTTGGCCTGAATCTTCGCGGCGATCGCCTCGGGGCt                                                                                  >  7:235594/1‑135 (MQ=255)
                                                             gATCAGCCCGGCGTGGTAACACAGCCCGTCGTAGGTGGAGTTGGTCACTACCGCCAGCTTGATACGCTGCCCACGATCACGGGCAAGGGGGTTGGCCTGAATCTTCGCGGCGATCGCCTCGGGGCTGAACTCGCt                                                                         <  5:13969/135‑1 (MQ=255)
                                                             gATCAGCCCGGCGTGGTAACACAGCCCGTCGTAGGGGGAGTTGGTCACTACCGCCAGCTTGATACGCTGCCCACGATCACGGGCAAGGGGGTTGGCCTGAATCTTCGCGGCGATCGCCTCGGGGCTGAACTCGCt                                                                         <  4:183720/135‑1 (MQ=255)
                                                                 aGCCCGGCGTGGTAACACAGCCCGTCGGAGGTGGAGTTGGTCAATCCCGCCAGCTTGATACGCTGCCCACGATCACGGGCAAGGGGGTTGGCCTGAATCTTCGCGGCGATCGCCTCGGGGCTGAACTCGCTGAGCg                                                                    >  8:125471/1‑136 (MQ=255)
                                                                  gCCCGGCGTGGTAACACAGCCCGTCGTAGGTGGAGTTGGTCACTACCGCCAGCTTGATACGCTGCCCACGATCACGGGCAAGGGGGTTGGCCTGAATCTTCGCGGCGATCGCCTCGGGGCTGAACTCGCTGAGCgg                                                                   <  8:57005/136‑1 (MQ=255)
                                                                  gCCCGGCGTGGTAACACAGCCCGTCGTAGGTGGAGTTGGTCACTACCGCCAGCTTGATACGCTGCCCACGATCACGGGCAAGGGGGTTGGCCTGAATCTTCGCGGCGATCGCCTCGGGGCTGAACTCGCTGAGCgg                                                                   <  3:301565/136‑1 (MQ=255)
                                                                  gCCCGGCGTGGTAACACAGCCCGTCGTAGGTGGAGTTGGTCACTACCGCCAGCTTGATACGCTGCCCACGATCACGGGCAAGGGGGTTGGCCTGAATCTTCGCGGCGATCGCCTCGGGGCTGAACTCGCTGAGCgg                                                                   <  2:1963/136‑1 (MQ=255)
                                                                      ggCGTGGTAACACAGCCCGTCGTAGGTGGAGTTGGTCACTACCGCCAGCTTGATACGCTGCCCACGATCACGGGCAAGGGGGTTGGCCTGAATCTTCGCGGCGATCGCCTCGGGGCTGAACTCGCTGAGCGGGATc                                                               >  3:101634/1‑136 (MQ=255)
                                                                      ggCGTGGTAACACAGCCCGTCGTAGGTGGAGTTGGTCACTACCGCCAGCTTGATACGCTGCCCACGATCACGGGCAAGGGGGTTGGCCCGAATCTTCGCGGCGATCGc                                                                                           <  1:181471/108‑1 (MQ=255)
                                                                      ggCGTGGTAACACAGCCCGTCGTAGGTGGAGTTGGTCACTACCGCCAGCTTGATACGCTGCCCACGATCACGGGCAAGGGGGTTGGCCCGAATCTTCGCGGCGATCGc                                                                                           >  2:181471/1‑108 (MQ=255)
                                                                       gCGTGGTAACACAGCCCGTCGTAGGTGGAGTTGGTCACTACCGCCAGCTTGATACGCTGCCCACGATCACGGGCAAGGGGGTTGGCCTGAATCTTCGCGGCGATCGCCTCGGGGCTGAACTCGCTGAGCGGGATCg                                                              <  8:153459/136‑1 (MQ=255)
                                                                       gCGTGGTAACACAGCCCGTCGTAGGTGGAGTTGGTCACTACCGCCAGCTTGATACGCTGCCCACGATCACGGGCAAGGGGGTTGGCCTGAATCTTCGCGGCGATCGCCTCGGGGCTGAACTCGCTGAGCGGGATCg                                                              <  2:119934/136‑1 (MQ=255)
                                                                           ggTAACACAGCCCGTCGTAGGTGGAGTTGGTCACTACCGCCAGCTTGATACGCTGCCCACGATCACGGGCAAGGGGGTTGGCCTGAATCTTCGCGGCGATCGCCTCGGGGCTGAACTCGCTGAGCGGGATCGGGc                                                           >  3:162882/1‑135 (MQ=255)
                                                                            gTAACACAGCCCGTCGTAGGTGGAGTTGGTCACTACCGCCAGCTTGATACGCTGCCCACGATCACGGGCAAGGGGGTTGGCCTGAATCTTCGCGGCGATCGCCTCGGGGCTGAACTCGCTGAGCGGGATCGGGCCa                                                         <  4:204680/136‑1 (MQ=255)
                                                                                cacaGCCCGTCGTAGGTGGAGTTGGTCACTACCGCCAGCTTGATACGCTGCCCACGATCACGGGCAAGGGGGTTGGCCTGAATCTTCGCGGCGATCGCCTCGGGGCTGAACTCGCTGAGCGGGATCGGGCCAatga                                                     >  1:154817/1‑136 (MQ=255)
                                                                                        gtcgtAGGTGGAGTTGGTCACTACCGCCAGCTTGATACGCTGCCTACGATCACGGGCAAGGGGGTTGGCCTGAATCTTCGCGGCGATCGCCTCGGGGCTGAACTCGCTGAGCGGGATCGGGCCAATGATGccc                                                <  1:141247/133‑1 (MQ=255)
                                                                                        gtcgtAGGTGGAGTTGGTCACTACCGCCAGCTTGATACGCTGCCTACGATCACGGGCAAGGGGGTTGGCCTGAATCTTCGCGGCGATCGCCTCGGGGCTGAACTCGCTGAGCGGGATCGGGCCAATGATGccc                                                <  1:136809/133‑1 (MQ=255)
                                                                                        gtcgtAGGTGGAGTTGGTCACTACCGCCAGCTTGATACGCTGCCTACGATCACGGGCAAGGGGGTTGGCCTGAATCTTCGCGGCGATCGCCTCGGGGCTGAACTCGCTGAGCGGGATCGGGCCAATGATGccc                                                >  2:141247/1‑133 (MQ=255)
                                                                                        gtcgtAGGTGGAGTTGGTCACTACCGCCAGCTTGATACGCTGCCTACGATCACGGGCAAGGGGGTTGGCCTGAATCTTCGCGGCGATCGCCTCGGGGCTGAACTCGCTGAGCGGGATCGGGCCAATGATGccc                                                >  2:136809/1‑133 (MQ=255)
                                                                                        gtcgtAGGTGGAGTTGGTCACTACCGCCAGCTTGATACGCTGCCCACGATCACGGGCAAGGGGGTTGGCCTGAATCTTCGCGGCGATCGCCTCGGGGCt                                                                                  >  4:118784/1‑99 (MQ=255)
                                                                                        gtcgtAGGTGGAGTTGGTCACTACCGCCAGCTTGATACGCTGCCCACGATCACGGGCAAGGGGGTTGGCCTGAATCTTCGCGGCGATCGCCTCGGGGCt                                                                                  <  3:118784/99‑1 (MQ=255)
                                                                                        gtcgtAGGTGGAGTTGGTCACTACCGCCAGCTTGATACGCTGCCCACGATCACGGGCAAGGGGGTTGGCCTGAATCTTCGCGGCGATCGCCTCGGGGCTGAACTCGCTGAGCGGGATCGGGCCAATGATGCCCAGt                                             >  4:115096/1‑136 (MQ=255)
                                                                                        gtcgtAGGTGGAGTTGGTCACTACCGCCAGCTTGATACGCTGCCCACGATCACGGGCAAGGGGGTTGGCCTGAATCTTCGCGGCGATCGCCTCGGGGCTGAACTCGCTGAGCGGGATCGGGCCAATGATGCCCAGt                                             >  7:24258/1‑136 (MQ=255)
                                                                                        gtcgtAGGTGGAGTTGGTCACTACCGCCAGCTTGATACGCTGCCCACGATCACGGGCAAGGGGGTTGGCCTGAATCTTCGCGGCGATCGCCTCGGGGCTGAACTCGCTGAGCGGGATCGGGCCAATGATGCCCAGt                                             >  7:51784/1‑136 (MQ=255)
                                                                                                      tGGTCACTACCGCCAGCTTGATACGCTGCCCACGa                                                                                                                                    <  2:147154/35‑1 (MQ=255)
                                                                                                      tGGTCACTACCGCCAGCTTGATACGCTGCCCACGa                                                                                                                                    >  1:147154/1‑35 (MQ=255)
                                                                                                        gTCACTACCGCCAGCTTGATACGCTGCCCACGATCACGGGCAAGGGGGTTGGCCTGAATCTTCGCGGCGATCGCCTCGGGGCTGAACTCGCTGAGCGGGATCGGGCCAATGATGCCCAGTTCGTTGCGCTCCggg                              <  6:72671/135‑1 (MQ=255)
                                                                                                                cGCCAGCTTGATACGCTGCCCACGATCACGGGCAAGGGGGTTGGCCTGAATCTTCGCGGCGATCGCCTCGGGGCTGAACTCGCTGAGCGGGATCGGGCCAATGATGCCCAGTTCGTTGCGCTCCGGGCACAGGTa                      >  1:266327/1‑135 (MQ=255)
                                                                                                                     gCTTGATACGCTGCCCACGATCACGGGCAAGGGGGTTGGCCTGAATCTTCGCGGCGATCGCCTCGGGGCTGAACTCGCTGAGCGGGATCGGGCCAATGATGCCCAGTTCGTTGCGCTCCGGGCACAGGTACAACgg                >  4:268982/1‑136 (MQ=255)
                                                                                                                          aTACGCTGCCCACGATCACGGGCAAGGGGGTTGGCCTGAATCTTCGCGGCGATCGCCTCGGGGCTGAACTCGCTGAGCGGGATCGGGCCAATGATGCCCAGTTCGTTGCGCTCCGGGCACAGGTACAACGGGATgg           >  4:41946/1‑136 (MQ=255)
                                                                                                                          aTACGCTGCCCACGATCACGGGCAAGGGGGTTGGCCTGAATCTTCGCGGCGATCGCCTCGGGGCTGAACTCGCTGAGCGGGATCGGGCCAATGATGCCCAGTTCGTTGCGCTCCGGGCACAGGTACAACGGGATgg           <  1:284606/136‑1 (MQ=255)
                                                                                                                           tACGATGCCCACGATCACGGGCAAGGGGGTTGGCCTGAATCTTCGCGGCGATCGCCTCGGGTCTGAACTCGCTGAGCGGGTTCGGGCCAATGATGCCCAGTTCGTTGCGCTCCGGGCACAGGTACAACGGGATgg           <  8:88470/135‑1 (MQ=255)
                                                                                                                                   ccACGATCACGGGCAAGGGGGTTGGCCTGAATCTTCGCGGCGATCGCCTCGGGGCTGAACTCGCTGAGCGGGATCGGGCCAATGATGCCCAGTTCGTTGCGCTCCGGGCACAGGTACAACGGGATGGCACCCGTc   >  6:227931/1‑135 (MQ=255)
                                                                                                                                   ccACGATCACGGGCAAGGGGGTTGGCCTGAATCTTCGCGGCGATCGCCTCGGGGCTGAACTCGCTGAGCGGGATCGGGCCAATGATGCCCAGTTCGTTGCGCTCCGGGCACAGGTACAACGGGATGGCACCCGTCa  >  7:228552/1‑136 (MQ=255)
                                                                                                                                   ccACGATCACGGGCAAGGGGGTTGGCCTGAATCTTCGCGGCGATCGCCTCGGGGCTGAACTCGCTGAGCGGGATCGGGCCAATGATGCCCAGTTCGTTGCGCTCCGGGCACAGGTACAACGGGATGGCACCCGTCa  >  3:289601/1‑136 (MQ=255)
                                                                                                                                    cACGATCACGGGCAAGGGGGTTGGCCTGAATCTTCGCGGCGATCGCCTCGGGGCTGAACTCGCTGAGCgg                                                                   <  1:225766/70‑1 (MQ=255)
                                                                                                                                    cACGATCACGGGCAAGGGGGTTGGCCTGAATCTTCGCGGCGATCGCCTCGGGGCTGAACTCGCTGAGCgg                                                                   >  2:225766/1‑70 (MQ=255)
                                                                                                                                    |                                                                                                                                      
CATAGGCAAACCAGGCCTCGTCGAAGTGCAGCACCTCGACACTGGCACCCAGGGCCTGCTTGATCAGCCCGGCGTGGTAACACAGCCCGTCGTAGGTGGAGTTGGTCACTACCGCCAGCTTGATACGCTGCCCACGATCACGGGCAAGGGGGTTGGCCTGAATCTTCGCGGCGATCGCCTCGGGGCTGAACTCGCTGAGCGGGATCGGGCCAATGATGCCCAGTTCGTTGCGCTCCGGGCACAGGTACAACGGGATGGCACCCGTCA  >  ACB143/4669089‑4669355

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑