Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 5,245,005 C→A 29.5% L177M (CTG→ATG)  gltC_4 → HTH‑type transcriptional regulator GltC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1435,245,0050CA29.5% 91.3 / 37.5 51L177M (CTG→ATG) gltC_4HTH‑type transcriptional regulator GltC
Reads supporting (aligned to +/- strand):  ref base C (11/25);  new base A (4/11);  total (15/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.63e-01

TCGAACAGGCCGTCAGAAGTGGCGAACTGGAACTGGGCGGCTGTTTGACGCCCACCGACGAGGCATTCGACTACCAGCCCTTCTGCAACGAACCGCTGGATGCCTTGCTGCCGGCTGGCCATGCTCTGGCGGGCCTGGACGCCGTGGGCCTGGGGCAACTGGCCGATACACCGTTCCTGCTGTACCAGCGCAGTTTCGTGCTCAACGACCGGCTGCTGAAGGCGTGCCAGCAGCAGGGCTTCACCCCCAAGGAAGGCGGGCGCAGCG  >  ACB143/5244871‑5245137
                                                                                                                                      |                                                                                                                                    
tCGAACAGGCCGTCAGACGTGGCGAACTGGAACTGGGCGGCTGTTTGACGCCCACCGACGAGGCAATCGACTACCAGCCCTTCTGCAACGAACCGCTGGATGCCTTGCTGCCGGCTGGCCATGCTCTGGCGGGCCt                                                                                                                                     <  8:38524/136‑1 (MQ=255)
tCGAACAGGCCGTCAGAAGTGGCGAACTGGACCTGCGCGTCTGTTTGACGCCCACCGACGAGGCATTCGACTACCAGCCCTTCTGCAACGAACCGCTGGATGCCTTGCTGCCGGCTGGCCATGCTCTGGCGGGCCt                                                                                                                                     <  7:168872/136‑1 (MQ=255)
tCGAACAGGCCGTCAGAAGTGGCGAACTGGAACTGGGCGGCTGTTTGACGCCCACCGACGAGGCATTCGACTACCAGCCCTTCTGCAACGAACCGCTGGATGCCTTGCTGCCGGCTGGCCATGCTCTGGCGGGCCt                                                                                                                                     <  3:99163/136‑1 (MQ=255)
 gtaaCAGGCCGTCAGTTGTGGCGAACTGTAACGGGGCGGCTGTTGGACGCCCACCGACGAGGCATTCGACTACCAGCCCTTCTGCAACGAACCGCTGGATGCCTTGCTGCCGGCTGGCCATGCTCTGGCGGGCCt                                                                                                                                     <  8:137074/133‑1 (MQ=255)
     cAGGCCGTCAGAAGTGGCGAACTGGAACTGGGCGGCTGTTTGACGCCCACCGACGAGGCATTCGACTACCAGCCCTTCTGCAACGAACCGCTGGATGCCTTGCTGCCGGCTGGCCATGCTCTGGCGGGCATGGACg                                                                                                                                <  8:238836/136‑1 (MQ=255)
      aGGCCTTCAGAAGTGGCTAACTGGAACTGGGCGGCTGTTTGACGCCCACCGACGAGGCATTCGACTACCAGCCCTTCTGCAACGAACCGCTGGATGCCTTGCTGCCTGCTGGCCATGCTCTGGCGGGCCTGGACGc                                                                                                                               <  8:255327/136‑1 (MQ=255)
      aGGCCGTCAGAAGTGGCGAACTGGAACTGGGCGGCTGTTTGACGCCCACCGACGAGGCATTCGACTACCAGCCCTTCTGCAACGAACCGCTGGATGCCTTGCTGCCGGCTGGCCATGCTCTGGCGGGCCTGGACGc                                                                                                                               <  6:140358/136‑1 (MQ=255)
           gTCAGAAGGGGCGAACTGTAACGGGGCGGCGGTTTGACGCCCACCGACGAGGCATTCGACTACCAGCCCTTCTGCAACGAACCGCTGGATGCCTTGCTGCCGGCTGGCCATGCTCTGGCGGGCCTGGACGCCGTgg                                                                                                                          <  2:118000/136‑1 (MQ=255)
             cAGAAGTGGCGAACTGGAACTGGGCGGCTGTTTGACGCCCACCGACGAGGCATTCGACTACCAGCCCTTCTGCAACGAACCGCTGGATGCCTTGCTGCCGGCTGGCCATGCTCTGGCGGGCCTGGACGCCGTGGGc                                                                                                                        >  6:199631/1‑136 (MQ=255)
              aGAAGTGGCGAACTGGAACTGGGCGGCTGTTTGACGCCCACCGACGAGGCATTCGACTACCAGCCCTTCTGCAACGAACCGCTGGATGCCTTGCTGCCGGCTGGCCATGCTCTGGCGGGCCTGGACGCCGTGGGcc                                                                                                                       <  4:19423/136‑1 (MQ=255)
                 aggggCGAACTGGAACTGGGCGGCTGTTTGACGCCCACCGACGAGGCATTCGACTACCAGCCCTTCTGCAACGAACCGCTGGATGCCTTGCTGCCGGCTGGCCATGCTCTGGCGGGCCTGGACGCCGTGGGCCTgg                                                                                                                    <  2:77521/133‑1 (MQ=255)
                 aGTGGCGAACTGGAACTGGGCGGCTGTTTGACGCCCACCGACGAGGCATTCGACTACCAGCCCTTCTGCAACGAACCGCTGGATGCCTTGCTGCCGGCTGGCCATGCTCTGGCGGGCCTGGACGCCGTGGGCCTgg                                                                                                                    <  7:156567/136‑1 (MQ=255)
                   tGGCGAACTGGAACTGGGCGGCTGTTTGACGCCCACCGACGAGGCATTCGACTACCAGCCCTTCTGCAACGAACCGCTGGATGCCTTGCTGCCGGCTGGCCATGCTCTGGCGGGCATGGACGCCGTGGGCCTgggg                                                                                                                  <  4:207475/136‑1 (MQ=255)
                   tGGCGAACTGGAACGGGGCGGCTGTTTGACGCCCACCGACGAGGCATTCGACTACCAGCCCTTCTGCAACGAACCGCTGGATGCCTTGCTGCCGGCTGGCCATGCTCTGGCGGGCATGGACGCCGTGGGCCTgggg                                                                                                                  <  7:229025/136‑1 (MQ=255)
                   tGGCGAACGGGACCTGGGAGGCTGTTGTCCGCCCACCGACGATGCATTCGACTACCAGCACTTCTGCAACGAACCGCTGGATGCCTTGCTGCCGGCTGGCCATGCTCTGGCGGGCATGGACGCCGTGGGCCTgggg                                                                                                                  <  2:224814/136‑1 (MQ=255)
                     tctacctggaactggGGGGGTGTTTGACGCCCACCGACGAGGCATTCGACTACCAGCCCTTCTGCAACGAACCGCTGGATGCCTTGCTGCAGGCTGGCCATGCTCTGGCGGGCCTGGACGCCGTGGGCCTGGGGCa                                                                                                                <  3:80743/131‑1 (MQ=255)
                        aactggaactggGCGGCGGTTTGACGCCCACCGACGAGGCATTCGACTACCAGCCCTTCTGCAACGAACCGCTGGATGCCTTGCTGCCGGCTGGCCATGCTCTGGCGGGCATGGACGCCGTGGGCCTGGGGCAACt                                                                                                             <  3:286321/136‑1 (MQ=255)
                              accggggCGGCTGTTTGACGCCCACCGACGAGGCATTCGACTACCAGCCCTTCTGCAACGAACCGCTGGATGCCTTGCTGCCGGCTGGCCATGCTCTGGCGGGCCTGGACGCCGTGGGCCTGGGGCAACTGGCCGa                                                                                                       <  5:1092/132‑1 (MQ=255)
                              aactggGCGGCTGTTTGACGCCCACCGACGAGGCATTCGACTACCAGCCCTTCTGCAACGAACCGCTGGATGCCTTGCTGCCGGCTGGCCATGCTCTGGCGGGCCTGGACGCCGTGGGCCTGGGGCAACTGGCCGa                                                                                                       <  5:307342/136‑1 (MQ=255)
                                ctggGCGGCTGTTTGACGCCCACCGACGAGTCATTCGACTACCAGCCCTTCTGCAACGAACCGCTGGATGCCTTGCTGCCGGCTGGCCATGCTCTGGCGGGCCTGGACGCCGTGGGCCTGGGGCAACTGGCCGATa                                                                                                     <  6:268221/136‑1 (MQ=255)
                                   ggcggcTGTTTGACGCCCACCGACGAGGCATTCGACTACCAGCCCTTCTGCAACGAACCGCTGGATGCCTTGCTGCCGGCTGGCCATGCTCTGGCGGGCCTGGACGCCGTGGGCCTGGGGCAACTGGCCGATacac                                                                                                  <  4:63113/136‑1 (MQ=255)
                                    gcggcTGTTTGACGCCCACCGACGAGGCATTCGACTACCAGCCCTTCTGCAACGAACCGCTGGATGCCTTGCTGCCGGCTGGCCATGCTCTGGCGGGCCTGGACGCCGTGGGCCTGGGGCAACTGGCCGATACAcc                                                                                                 <  6:16075/136‑1 (MQ=255)
                                    gcggaggTTTGACGCCCACCGACGAGGCATTCGACTACCAGCCCTTCTGCAACGAACCGCTGGATGCCTTGCTGCCGGCTGGCCATGCTCTGGCGGGCATGGACGCCGTGGGCCTGGGGCAACTGGCCGATACAcc                                                                                                 <  1:112056/130‑1 (MQ=255)
                                         tGTTTGACGCCCACCGACGAGGCATTCGACTACCAGCCCTTCTGCAACGAACCGCTGGATGCCTTGCTGCCGGCTGGCCATGCTCTGGCGGGCCGGGACGCCGTGGGCCTGGGGCAACTGGCCGATACACCGTTcc                                                                                            <  8:88732/136‑1 (MQ=255)
                                           tttGACGCCCACCGACGAGGCATTCGACTACCAGCCCTTCTGCAACGAACCGCTGGATGCCTTGCTGCCGGCTGGCCATGCTCTGGCGGGCCTGGACGCCGTGGGCCTGGGGCAACTGGCCGATACACCGTTCctg                                                                                          >  3:86182/1‑136 (MQ=255)
                                           tttGACGCCCACCGACGAGGCATTCGACTACCAGCCCTTCTGCAACGAACCGCTGGATGCCTTGCTGCCGGCTGGCCATGCTCTGGCGGGCATGGACGCCGTGGGCCTGGGGCAACTGGCCGATACACCGTTCctg                                                                                          >  1:91921/1‑136 (MQ=255)
                                              gACGCCCCCCGACGAGGCATTCGACTACCAGCCCTTCTGCAACGAACCGCTGGATGCCTTGCTGCCGGCTGGCCATGCTCTGGCGGGCCTGGACGCCGTGGGCCTGGGGCAACTGGCCGATACACCGTTCctgctg                                                                                       <  5:31717/136‑1 (MQ=255)
                                                      ccGACGAGGCATTCGACTACCAGCCCTTCTGCAACGAACCGCTGGATGCCTTGCTGCCGGCTGGCCATGCTCTGGCGGGCCTGGACGCCGTGGGCCTGGGGCAACTGGCCGATACACCGTTCCTGCTGTACCa                                                                                  <  5:101510/133‑1 (MQ=255)
                                                      ccGACGAGGCATTCGACTACCAGCCCTTCTGCAACGAACCGCTGGATGCCTTGCTGCCGGCTGGCCATGCTCTGGCGGGCCTGGACGCCGTGGGCCTGGGGCAACTGGCCGATACACCGTTCCTGCTGTACCa                                                                                  >  6:101510/1‑133 (MQ=255)
                                                        gacgaGGCATTCGACTACCAGCCCTTCTGCAACGAACCGCTGGATGCCTTGCTGCCGGCTGGCCATGCTCTGGCGGGCATGGACGCCGTGGGCCTGGGGCAACTGGCCGATACACCGTTCCTGCTGTACCAgcg                                                                               >  4:53798/1‑134 (MQ=255)
                                                        gacgaGGCATTCGACTACCAGCCCTTCTGCAACGAACCGCTGGATGCCTTGCTGCCGGCTGGCCATGCTCTGGCGGGCATGGACGCCGTGGGCCTGGGGCAACTGGCCGATACACCGTTCCTGCTGTACCAgcg                                                                               >  8:64559/1‑134 (MQ=255)
                                                           gaGGCATTCGACTACCAGCCCTTCTGCAACGAACCGCTGGATGCCTTGCTGCCGGCTGGCCATGCTCTGGCGGGCCTGGACGCCGTGGGCCTGGGGCAACTGGCCGATACACCGTTCCTGCTGTACCAGCGCAGtt                                                                          <  8:42370/136‑1 (MQ=255)
                                                           gaGGCATTCGACTACCAGCCCTTCTGCAACGAACCGCTGGATGCCTTGCTGCCGGCTGGCCATGCTCTGGCGGGCATGGACGCCGTGGGCCTGGGGCAACTGGCCGATACACCGTTCCTGCTGTACCAGCGCAGtt                                                                          <  8:116359/136‑1 (MQ=255)
                                                                         ccAGCCCAACTCCAACGAACCGCTGGAAGCCTTGCTGCCGTCTGGCCATGCACTGGCGGGCCTGGACGCCGTGGGCCTGGGGCAACTGGCCGATACACCGTTCCTGCTCTACCAGCGCAGTTTCGTGCTCAACGAc                                                            <  2:298351/136‑1 (MQ=255)
                                                                                    gCAACGAACCGCTGGATGCCTTGCTGCCGGCTGGCCATGCTCTGGCGGGCCTGGACGCCGTGGGCCTGGGGCAACTGGCCGATACACCGTTCCTGCTGTACCAGCGCAGTTTCGTGCTCAACGACCGGctgctg                                                   >  1:178647/1‑134 (MQ=255)
                                                                                    gCAACGAACCGCTGGATGCCTTGCTGCCGGCTGGCCATGCTCTGGCGGGCCTGGACGCCGTGGGCCTGGGGCAACTGGCCGATACACCGTTCCTGCTGTACCAGCGCAGTTTCGTGCTCAACGACCGGctgctg                                                   >  7:122231/1‑134 (MQ=255)
                                                                                    gCAACGAACCGCTGGATGCCTTGCTGCCGGCTGGCCATGCTCTGGCGGGCATGGACGCCGTGGGCCTGGGGCAACTGGCCGATACACCGTTCCTGCTGTACCAGCGCAGTTTCGTGCTCAAc                                                               >  2:36173/1‑122 (MQ=255)
                                                                                    gCAACGAACCGCTGGATGCCTTGCTGCCGGCTGGCCATGCTCTGGCGGGCATGGACGCCGTGGGCCTGGGGCAACTGGCCGATACACCGTTCCTGCTGTACCAGCGCAGTTTCGTGCTCAAc                                                               <  1:36173/122‑1 (MQ=255)
                                                                                     cAACGAACCGCTGGATGTCTTGCTGCCGGCTGGCCATGCTCTGGCGGGCATGGACGCCGTGGGCCTGGGGCAACTGGCCGATACACCGTTCCTGCTGTACCAGCGCAGTTTCGTGCTCAACGACCGGCTGCTGaa                                                 <  3:128450/135‑1 (MQ=255)
                                                                                         gAACCGCTGGATGCCTTGCTGCCGGCTGGCCATGCTCTGGCGGGCCTGGACGCCGTGGGCCTGGGGCAACTGGCCGATACACCGTTCCTGCTGTACCAGCGCAGTTTCGTGCTCAACGACCGGCTGCTGAAGGCGt                                            <  2:314232/136‑1 (MQ=255)
                                                                                             cGCTGGATGCCTTGCTGCCGGCTGGCCATGCTCTGGCGGGCCTGGACGCCGTGGGCCTGGGGCAACTGGCCGATACACCGTTCCTGCTGTACCAGCGCAGTTTCGTGCTCAACGACCGGCTGCTGAAGGCGTGc                                          >  4:217212/1‑134 (MQ=255)
                                                                                               cTGGATGCCTTGCTGCCGGCTGGCCATGCTCTGGCGGGCCTGGACGCCGTGGGCCTGGGGCAACTGGCCGATACACCGATCCTGCTGTACCAGCGCAGTTTCGTGCTCAACGACCGGCTGCTGAAGGCGTGCcagc                                      <  8:185523/136‑1 (MQ=255)
                                                                                                      ccTTGCTGCCGGCTGGCCATGCTCTGGCGGGCATGGACGCCGTGGGCCTGGGGCAACTGGCCGATACACCGTTCCTGCTGTACCAGCGCAGTTTCGTGCTCAACGACCGGCTGCTGAAGGCGTGCCAGCAGCAggg                               <  8:137872/136‑1 (MQ=255)
                                                                                                      ccTTGCTGCCGGCTGGCCATGCTCTGGCGGGCATGGACGCCGTGGGCCTGGGGCAACTGGCCGATACACCGTTCCTGCTGTACCAGCGCAGTTTCGTGCTCAACGACCGGCTGCTGAAGGCGTGCCAGCAGCAggg                               <  6:148896/136‑1 (MQ=255)
                                                                                                          gctgcCGGCTGGCCATGCTCTGGCGGGCCTGGACGCCGTGGGCCTGGGGCAACTGGCCGATACACCGTTCCTGCTGTACCAGCGCAGTTTCGTGCTCAACGACCGGCTGCTGAAGGCGTGCCAGCAGCAGGGCtt                            >  8:58371/1‑135 (MQ=255)
                                                                                                            tgcCGGCTGGCCATGCTCTGGCGGGCCTGGACGCCGTGGGCCTGGGGCAACTGGCCGATACACCGTTCCTGCTGTACCAGCGCAGTTTCGTGCTCAACGACCGGCTGCTGAAGGCGTGCCAGCAGCAGGGCTTCAc                         >  7:118888/1‑136 (MQ=255)
                                                                                                              ccGGCTGGCCATGCTCTGGCGGGCCTGGACGCCGTGGGCCTGGGGCAACTGGCCGATACACCGTTCCTGCTGTACCAGCGCAGTTTCGTGCTCAACGACCGGCTGCTGAAGGCGTGCCAGCAGCAGGGCTTCAc                         <  4:302413/134‑1 (MQ=255)
                                                                                                                           ctctGGCGGGCCTGGACGCCGTGGGCCTGGGGCAACTGGCCGATACACCGTTCCTGCTGTACCAGCGCAGTTTCGTGCTCAACGACCGGCTGCTGAAGGCGTGCCAGCAGCAGGGCTTCACCCCCAAGGAAggcgg          <  2:257030/136‑1 (MQ=255)
                                                                                                                                   ggCCTGGACGCCGTGGGCCTGGGGCAACTGGCCGATACACCGTTCCTGCTGTACCAGCGCAGTTTCGTGCTCAACGACCGGCTGCTGAAGGCGTGCCAGCAGCAGGGCTTCACCCCCAAGGAAGGCGGGCGCAGCg  >  3:161717/1‑136 (MQ=255)
                                                                                                                                   ggCCTGGACGCCGTGGGCCTGGGGCAACTGGCCGATACACCGTTCCTGCTGTACCAGCGCAGTTTCGTGCTCAACGACCGGCTGCTGAAGGCGTGCCAGCAGCAGGGCTTCACCCCCAAGGAAGGCGGGCGCAGCg  >  3:52475/1‑136 (MQ=255)
                                                                                                                                   ggCCTGGACGCCGTGGGCCTGGGGCAACTGGCCGATACACCGTTCCTGCTGTACAAGCGCAGTTTCGTGCTCAACGACCGGCTGCTGAAGGCGTGCCAGCAGCAGGGCTTCACCCCCAAGGAAGGCGGGCGCAGCg  >  8:282366/1‑136 (MQ=255)
                                                                                                                                      |                                                                                                                                    
TCGAACAGGCCGTCAGAAGTGGCGAACTGGAACTGGGCGGCTGTTTGACGCCCACCGACGAGGCATTCGACTACCAGCCCTTCTGCAACGAACCGCTGGATGCCTTGCTGCCGGCTGGCCATGCTCTGGCGGGCCTGGACGCCGTGGGCCTGGGGCAACTGGCCGATACACCGTTCCTGCTGTACCAGCGCAGTTTCGTGCTCAACGACCGGCTGCTGAAGGCGTGCCAGCAGCAGGGCTTCACCCCCAAGGAAGGCGGGCGCAGCG  >  ACB143/5244871‑5245137

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑