Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 5,390,208 G→A 28.0% G101D (GGC→GAC)  ligH → 10‑formyltetrahydrofolate synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1435,390,2080GA28.0% 57.6 / 21.7 36G101D (GGC→GAC) ligH10‑formyltetrahydrofolate synthetase
Reads supporting (aligned to +/- strand):  ref base G (14/11);  new base A (6/4);  total (21/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

TGATCCTCGTCACCGCGATCAACCCGACGCCGGCCGGCGAAGGCAAGACCACGACCACCGTGGGCCTGGCCGACGGCCTCAACCGCATCGGCAAGAAGACCGTGGCGGCGCTGCGCGAGCCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAGGTCGTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGACTTCCATGCCATCACCTCGGCGAACAACCTGCTC  >  ACB143/5390079‑5390332
                                                                                                                                 |                                                                                                                            
tGATCCTCGTCACCGCGATCAACCCGACGCCGGCCGGCGAAGGCAAGACCACGACCACCGTGGGCCTGGCCGACGGCCTCCACCGCATCGGCTAGAAGACAGTGGCGGCGCTGCGCGCGCCGTCGCTCGTCCCCTg                                                                                                                        >  4:240913/1‑136 (MQ=255)
   gccTCGTCACCGCTATCAACCCCACGCCGGCCGGCGAAGGAAAGACCACGACCACCGTGGGCCTGGCCGACGGCCTCAACCGCATCGGCAAGAAGACCGTGGCGGCGCTGCGCGAGCCTTCGCTCGGCCCCTGttt                                                                                                                     <  7:204523/135‑1 (MQ=255)
     cTCGTCACCGCGATCAACCCGACGCCGGCCGGCGAAGGCAAGACCACGACCACCGTGGGCCTGGCCGACGGCCTCAACCGCATCGGCAAGAAGACCGTGGCGGCGCTGCGCGAGCCTTCGCTCGGCCCCTGTTTCg                                                                                                                   >  5:108507/1‑136 (MQ=255)
                           cGCCGGCCGGCGAAGGCAAGACCACGACCACCGTGGGCCTGGCCGACGGCCTCAACCGCATCGGCAAGAAGACCGTGGCGGCGCTGCGCGAGCCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGcggccgg                                                                                             <  4:101592/136‑1 (MQ=255)
                           cGCCGGCCGGCGAAGGCAAGACCACGACCACCGTGGGCCTGGCCGACGGCCTCAACCGCATCGGCAAGAAGACCGTGGCGGCGCTGCGCGAGCCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGcggccgg                                                                                             <  3:131413/136‑1 (MQ=255)
                             ccggccggCGAAGGCAAGACCACGACCACCGTGGGCCTGGCCGACGGCCTCAACCGCATCGGCAAGAAGACCGTGGCGGCGCTGCGCGAGCCTTCGCTCGACCCCTGTTTCGGCGTGAAGGGCGGCGCGGCcggcg                                                                                           >  4:166769/1‑136 (MQ=255)
                              cggccggcGAAGGCAAGACCACGACCACCGTGGGCCTGGCCGACGGCCTCAACCGCATCGGCAAGAAGACCGTGGCGGCGCTGCGCGAGCCTTCGCTCGACCCCTGTTTCGGCGTGAAGGGCGGCGCGGCcggcgg                                                                                          <  3:166769/136‑1 (MQ=255)
                                gccggcGAAGGCAAGACCACGACCACCGTGGGCCTGGCCGACGGCCTCAACCGCATCGGCAAGAAGACCGTGGCGGCGCTGCGCGAGCCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCcggcggcg                                                                                        <  3:154433/136‑1 (MQ=255)
                                   ggcGAAGGCAAGACCACGACCACCGTGGGCCTGGCCCACGGCCTCAACCGCAACGGCAAGAAGACCGTGGCGGCGCTGCGCCACCCATCGCACGGCCCCAGTTTCGGCGTGAAGGGCGGCGCGGCCggcggcggc                                                                                      >  8:265898/1‑135 (MQ=255)
                                                      ccaccGTGGGCCTGGCCGACGGCCTCAACCGCATCGGCAAGAAGACCGTGGCGGCGCTGCGCGAGCCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAGGTCGTGCCGag                                                                  >  4:156663/1‑135 (MQ=255)
                                                      ccaccGTGGGCCTGGCCGACGGCCTCAACCGCATCGGCAAGAAGACCGTGGCGGCGCTGCGCGAGCCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAGGTCGTGCCGAt                                                                  >  6:296787/1‑136 (MQ=255)
                                                      ccaccGTGGGCCTGGCCGACGGCCTCAACCGCATCGGCAAGAAGACCGTGGCGGCGCTGCGCGAGCCTTCGCTCGACCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAGGTCGTGCCGAt                                                                  >  2:4639/1‑136 (MQ=255)
                                                             gggCCTGGCCGACGGCCTCAACCGCATCGGCAAGAAGACCGTGGCGGCGCTGCGCGAGCCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAGGTCGTGCCGATggaggcc                                                           >  3:6200/1‑134 (MQ=255)
                                                                ccTGGCCGACGGCCTCAACCGCATCGGCAAGAAGACCGTGGCGGCGCTGCGCGAGCCTTCGCTCGACCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAGGTCGTGCCGATGGAGGACATc                                                        <  4:100582/136‑1 (MQ=255)
                                                                 cTGGCCGACGGCCTCAACCGCATCGGCAAGAAGACCGTGGCGGCGCTGCGCGAGCCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAGGTCGTGCCGATGGAGGACATc                                                        >  4:488/1‑135 (MQ=255)
                                                                    gCCGACGGCCTCAACCGCATCGGCAAGAAGACCGTGGCGGCGCTGCGCGAGCCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAGGTCGTGCCGATGGAGGACATCAAc                                                     <  1:321587/135‑1 (MQ=255)
                                                                     ccGACGGCCTCAACCGCATCGGCAAGAAGACCGTGGCGGCGCTGCGCGAGCCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAGGTCGTGCCGATGGAGGACATCAACct                                                   <  4:260951/136‑1 (MQ=255)
                                                                       gACGGCCTCAACCGCATCGGCAAGAAGACCGTGGCGGCGCTGCGCGAGCCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAGGTCGTGCCGATGGAGGACATCAACctt                                                  >  5:118575/1‑135 (MQ=255)
                                                                       gACGGCCTCAACCGCATCGGCAAGAAGACCGTGGCGGCGCTGCGCGAGCCGTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCGATGCGCAGGTCGGGCCGATGGAGGACATCAACctt                                                  >  1:316053/1‑135 (MQ=255)
                                                                        aCGGCCTCAACCGCATCGGCAAGAAGACCGTGGCGGCGCTGCGCGAGCCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAGGTCGTGCCGATGGAGGACATCAACcttca                                                <  8:92310/136‑1 (MQ=255)
                                                                            ccTCAACCGCATCGGCAAGAAGACCGTGGCGGCGCTGCGCGAGCCTTCGCTCGACCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAGGTCGTGCCGATGGAGGACATCAACcttcactt                                             >  1:145697/1‑135 (MQ=255)
                                                                                  ccGCATCGGCAAGAAGACCGTGGCGGCGCTGCGCGAGCCTTCGCTCGGCCCCTGTTTCGGCGTGTAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAGGTCGTGCCGATGGAGGACATCAACCTTCACTTCACCGGt                                      >  3:123323/1‑136 (MQ=255)
                                                                                      aTCGGCAAGAAGACCGTGGCGGCGCTGCGCGAGCCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAGGTCGTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGACt                                  <  7:13679/136‑1 (MQ=255)
                                                                                      aTCGGCAAGAAGACCGTGGCGGCGCTGCGCGAGCCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAGGTCGTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGACt                                  <  7:265898/136‑1 (MQ=255)
                                                                                         ggCAAGAAGTCCGTGGCGGCGCTGCGCGAGCCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAGGTCGTGCCGATGGAGGAGATCAACCTTCACTTCACCGGTGACtt                                 <  2:263707/134‑1 (MQ=255)
                                                                                                   ccGTGGCGGCGCTGCGCGAGCCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAGGTCGTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGACTTCCATGCCATCAc                     <  8:64893/136‑1 (MQ=255)
                                                                                                   ccGTGGCGGCGCTGCGCGAGCCTTCGCTCGACCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAGGTCGTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGACTTCCATGCCATCAc                     <  2:12456/136‑1 (MQ=255)
                                                                                                       ggcggcGCTGCGCGAGCCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAGGTCGTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGACTTCCATGCCATCACCTCg                 >  7:287669/1‑136 (MQ=255)
                                                                                                       ggcggcGCTGCGCGAGCCTTCGCTCGACCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAGGTCGTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGACTTCCATGCCATCACCTCg                 >  1:199265/1‑136 (MQ=255)
                                                                                                       ggcggcGCTGCGCGAGCCTTCGCTCGACCCCTGTTTCGGCGTGAAGGGCGGCGCGGACGGCGGCGGCTATGCGCAGGTCGTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGACTTCCATGCCATCACCTCg                 >  7:222206/1‑136 (MQ=255)
                                                                                                              cTGCGCGAGCCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAGGTCGTGCCGATGGAGGACCTCCACCTTCAATTAACCGGTGACTTCCATGCCATCACCTCGGCGaac           >  2:298944/1‑135 (MQ=255)
                                                                                                               tGCGCGAGCCTTCGCTCGACCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAGGTCGTGCCGATGGAGGACATCACCCTTCACTTCACCGGTGACTTCCATGCCATCACCTCGGCGaacaa         >  3:270380/1‑136 (MQ=255)
                                                                                                                 cgcgAGCCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAGGTCGTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGACTTCCATGCCATCACCTCGGCGAACAAcc       >  8:88031/1‑136 (MQ=255)
                                                                                                                    gcgCCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAGGTCGTGCCGATGGAGGCCATCCACCTTCACTTCACCGGTGACTTCCATGCCATCCCCGCGGCGAACAACCTGc    >  4:3229/3‑136 (MQ=255)
                                                                                                                    gAGCCTTCGCTCGACCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAGGTCGTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGACTTCCATGCCATCACCTCGGCGAACAACCTGc    <  7:113955/136‑1 (MQ=255)
                                                                                                                      gCCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAGGTCGTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGACTTCCATGCCATCACCTCGGCGAACAACCTGCTc  >  3:213387/1‑136 (MQ=255)
                                                                                                                                 |                                                                                                                            
TGATCCTCGTCACCGCGATCAACCCGACGCCGGCCGGCGAAGGCAAGACCACGACCACCGTGGGCCTGGCCGACGGCCTCAACCGCATCGGCAAGAAGACCGTGGCGGCGCTGCGCGAGCCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAGGTCGTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGACTTCCATGCCATCACCTCGGCGAACAACCTGCTC  >  ACB143/5390079‑5390332

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑