Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB143	5,667,963	C→T	7.0%	Q91* (CAG→TAG)	CJ019_05320 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB143	5,667,963	0	C	T	7.0%	233.0 / 4.7	71	Q91* (CAG→TAG)	CJ019_05320	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (40/26);  new base T (2/3);  total (42/29)
Fisher's exact test for biased strand distribution p-value = 3.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.84e-01

ATCCAGGTCTACACCGACAATGCGCAGCCATACGCCAGGCTGCCCGTCACCGTCCACCTGCTGGACGAGGCCACGCGCCAGGCATGGAATGCCCCGCACGGTTATCACTTTCGCAGCAAGCATGTGCTGTTGCGCCAGGTACTGCAGCAACACCCGCTGGCAGTGCTGATCGACACCGACACCTTCTTCCGCACTTCGCCGTTGCATCTGTTCGCCCGCGTGGCGCCCAACCGCCTGCTGTGCAACGCCATCGGCACGCGCTACGGCG  >  ACB143/5667828‑5668095                                                                                                                                        |                                                                                                                                     aTCCAGGTCTACACCGACAATGCGCAGCCATACGCCAGGCTGCCCGTCACCGTCCACCTGCTGGACGAGGCCACGCGCCAGGCATGGAATGCCCCGCACGGTTATCACTTTCGCAGCAAGCATGTGCTGTTGCGcc                                                                                                                                      <  2:266917/136‑1 (MQ=255)    cAGGTCTACACCGAGAATGCGCAGACATACTCCAGGCTGCCCGTCACCGTCCACCTGCTGGACGAGGCCACGCGCCAGGCATGGAATGCCCCGCACGGTTATCACTTTCGCAGCAAGCATGTGCTGTTGCGCTAgg                                                                                                                                   <  7:122966/136‑1 (MQ=255)     aGGTCTACACCGACAATGCGCAGCCATACGCCAGGCTGCCCGTCACCGTCCACCTGCTGGACGAGGCCACGCGCCAGGCATGGAATGCCCCGCACGGTTATCACTTTCGCAGCAAGCATGTGCTGTTGCGCCAgg                                                                                                                                   >  6:21005/1‑135 (MQ=255)      ggTCTACACCGACAATGCGCAGCCATACGCCAGGCTGCCCGTCACCGTCCACCTGCTGGACGAGGCCACGCGCCAGGCATGGAATGCCCCGCACGGTTATCACTTTCGCAGCAAGCATGTGCTGTTGCGCCAGGTa                                                                                                                                 >  7:75049/1‑136 (MQ=255)      ggTCTACACCGACAATGCGCAGCCATACGCCAGGCTGCCCGTCACCGTCCACCTGCTGGACGAGGCCACGCGCCAGGCATGGAATGCCCCGCACGGTTATCACTTTCGCAGCAAGCATGTGCTGTTGCGCCAGGTa                                                                                                                                 >  8:174239/1‑136 (MQ=255)        tCTACACCGACAATGCGCAGCCATACGCCAGGCTGCCCGTCACCGTCCACCTGCTGGACGAGGCCACGCGCCAGGCATGGAATGCCCCGCACGGTTATCACTTTCGCAGCAAGCATGTGCTGTTGCGCCAGGTACt                                                                                                                               <  8:223415/136‑1 (MQ=255)             acCGACAATGCGCAGCCATACGCCAGGCTGCCCGTCCCCGTCCACCTGCTGGACGAGGCCACGCGCCAGGCATGGAATGCCCCGCACGGTTATCACTTTCGCAGCAAGCATGTGCTGTTGCGCCAGGTACTgcag                                                                                                                           >  5:125281/1‑135 (MQ=255)             acCGACAATGCGCAGCCATACGCCAGGCTGCCCGTCACCGTCCACCTGCTGGACGAGGCCACGCGCCAGGCATGGAATGCCCCGCACGGTTATCACTTTCGCAGCAAGCATGTGCTGTTGCGCCAGGTACTgcag                                                                                                                           >  5:158116/1‑135 (MQ=255)             acCGACAATGCGCAGCCATACGCCAGGCTGCCCGTCACCGTCCACCTGCTGGACGAGGCCACGCGCCAGGCATGGAATGCCCCGCACGGTTATCACTTTCGCAGCAAGCATGTGCTGTTGCGCCAGGTACTgcag                                                                                                                           >  4:131467/1‑135 (MQ=255)               cGACAATGCGCAGCCATACGCCAGGCTGCCCGTCACCGTCCACCTGCTGGACGAGGCCACGCGCCAGGCATGGAATGCCCCGCACGGTTATCACTTTCGCAGCAAGCATGTGCTGTTGCGCCAGGTACTGCAGCaa                                                                                                                        >  3:84701/1‑136 (MQ=255)                 aCAATGCGCAGCCATACGCCAGGCTGCCCGTCACCGTCCACCTGCTGGACGAGGCCACGCGCCAGGCATGGAATGCCCCGCACGGTTATCACTTTCGCAGCAAGCATGTGCTGTTGCGCCAGGTACTGCAGCAaca                                                                                                                      <  8:128609/136‑1 (MQ=255)                    aTGCGCAGCCATACGCCAGGCTGCCCGTCACCGTCCACCTGCTGGACGAGGCCACGCGCCAGGCATGGAATGCCCCGCACGGTTATCACTTTCGCAGCAAGCATGTGCTGTTGCGCCAGGTACTGCAGCAACAccc                                                                                                                   >  6:207201/1‑136 (MQ=255)                    aTGCGCAGCCATACGCCAGGCTGCCCGTCACCGTCCACCTGCTGGACGAGGCCACGCGCCAGGCATGGAATGCCCCGCACGGTTATCACTTTCGCAGCAAGCATGTGCTGTTGCGCCAGGTACTGCAGCAACAccc                                                                                                                   >  3:41461/1‑136 (MQ=255)                    aTGCGCAGCCATACGCCAGGCTGCCCGTCACCGTCCACCTGCTGGACGAGGCCACGCGCCAGGCATGGAATGCCCCGCACGGTTATCACTTTCGCAGCAAGCATGTGCTGTTGCGCCAGGTACTGCAGCAACAccc                                                                                                                   >  6:23949/1‑136 (MQ=255)                    aTGCGCAGCCATACGCCAGGCTGCCCGTCACCGTCCACCTGCTGGACGAGGCCACGCGCCAGGCATGGAATGCCCCGCACGGTTATCACTTTCGCAGCAAGCATGTGCTGTTGCGCCAGGTACTGCAGCAACAccc                                                                                                                   >  8:37365/1‑136 (MQ=255)                    aTGCGCAGCCATACGCCAGGCTGCCCGTCACCGTCCACCTGCTGGACGAGGCCACGCGCCAGGCATGGAATGCCCCGCACGGTTATCACTTTCGCAGCAAGCATGTGCTGTTGCGCCAGGTACTGCAGCAACAccc                                                                                                                   >  5:131432/1‑136 (MQ=255)                    aTGCGCAGCCATACGCCAGGCTGCCCGTCACCGTCCACCTGCTGGACGAGGCCACGCGCCAGGCATGGAATGCCCCGCACGGTTATCACTTTCGCAGCAAGCATGTGCTGTTGCGCCAGGTACTGCAGCAACAccc                                                                                                                   >  1:272209/1‑136 (MQ=255)                    aTGCGCAGCCATACGCCAGGCTGCCCGTCACCGTCCACCTGCTGGACGAGGCCACGCGCCAGGCATGGAATGCCCCGCACGGTTATCACTTTCGCAGCAAGCATGTGCTGTTGCGCCAGGTACTGCAGCAACAccc                                                                                                                   >  2:193916/1‑136 (MQ=255)                    aTGCGCAGCCATACGCCAGGCTGCCCGTCACCGTCAACCTGCTGGACGAGGCCACGCGCCAGGCATGGAATGCCCCGCACGGTTATCACTTTCGCAGCAAGCATGTGCTGTTGCGCCAGGTACTGCAGCAACAccc                                                                                                                   >  7:146909/1‑136 (MQ=255)                             cATACGCCAGGCTGCCCGTCACCGTCCACCTGCTGGACGAGGCCACGCGCCAGGCATGGAATGCCCCGCACGGTTATCACTTTCGCAGCAAGCATGTGCTGTTGCGCCAGGTACTGCAGCAACACCCGCTGGCAGt                                                                                                          >  2:4909/1‑136 (MQ=255)                                 cGCCAGGCTGCCCGTCACCGTCCACCTGCTGGACGAGGCCACGCGCCAGGCATGGAATGCCCCGCACGGTTATCACTTTCGCAGCAAGCATGTGCTGTTGCGCCAGGTACTGCAGCAACACCCGCTGGCAGTGCTg                                                                                                      <  2:195420/136‑1 (MQ=255)                                  gCCAGGCTGCCCGTCACCGTCCACCTGCTGGACGAGGCCACGCGCCAGGCATGGAATGCCCCGCACGGTTATCACTTTCGCAGCAAGCATGTGCTGTTGCGCCAGGTACTGCAGCAACACCCGCTGGCAGTGCTGa                                                                                                     >  2:300740/1‑136 (MQ=255)                                       gCTGCCCGTCACCGTCCACCTGCTGGACGAGGCCACGCGCCAGGCATGGAATGCCCCGCACGGTTATCACTTTCGCAGCAAGCATGTGCTGTTGCGCCAGGTACTGCAGCAACACCCGCTGGCAGTGCTGATcga                                                                                                 >  3:37201/1‑135 (MQ=255)                                         tGCCCGTCACCGTCCACCTGCTGGACGAGGCCACGCGCCAGGCATGGAATGCCCCGCACGGTTATCACTTTCGCAGCAAGCATGTGCTGTTGCGCTAGGTACTGCAGCAACACCCGCTGGCAGTGCTGATcgacac                                                                                              >  1:212994/1‑136 (MQ=255)                                         tGCCCGTCACCGTCCACCTGCTGGACGAGGCCACGCGCCAGGCATGGAATGCCCCGCACGGTTATCACTTTCGCAGCAAGCATGTGCTGTTGCGCCAGGTACTGCAGCAACACCCGCTGGCAGTGCTGATcgacac                                                                                              >  5:191891/1‑136 (MQ=255)                                         tGCCCGTCACCGTCCACCTGCTGGACGAGGCCACGCGCCAGGCATGGAATGCCCCGCACGGTTATCACTTTCGCAGCAAGCATGTGCTGTTGCGCCAGGTACTGCAGCAACACCCGCTGGCAGTGCTGATcgacac                                                                                              >  7:90380/1‑136 (MQ=255)                                         tGCCCGTCACCGTCCACCTGCTGGACGAGGCCACGCGCCAGGCATGGAATGCCCCGCACGGTTATCACTTTCGCAGCAAGCATGTGCTGTTGCGCCAGGTACTGCAGCAACACCCGCTGGCAGTGCTGATcgacac                                                                                              >  7:115244/1‑136 (MQ=255)                                         tGCCCGTCACCGTCCACCTGCTGGACGAGGCCACGCGCCAGGCATGGAATGCCCCGCACGGTTATCACTTTCGCAGCAAGCATGTGCTGTTGCGCCAGGTACTGCAGCAACACCCGCTGGCAGTGCTGATcgacac                                                                                              >  6:271989/1‑136 (MQ=255)                                         tGCCCGTCACCGTCCACCTGCTGGACGAGGCCACGCGCCAGGCATGGAATGCCCCGCACGGTTATCACTTTCGCAGCAAGCATGTGCTGTTGCGCCAGGTACTGCAGCAACACCCGCTGGCAGTGCTGATcgacac                                                                                              >  7:178343/1‑136 (MQ=255)                                         tGCCCGTCACCGTCCACCTGCTGGACGAGGCCACGCGCCAGGCATGGAATGCCCCGCACGGTTATCACTTTCGCAGCAAGCATGTGCTGTTGCGCCAGGTACTGCAGCAACACCCGCTGGCAGTGCTGATcgacac                                                                                              >  1:225372/1‑136 (MQ=255)                                         tGCCCGTCACCATCCACCTGCTGGACGAGGCCACGCGCCAGGCATGGAATGCCCCGCACGGTTATCACTTTCGCAGCAAGCATGTGCTGTTGCGCCAGGTACTGCAGCAACACCCGCTGGCAGTGCTGATcgacac                                                                                              >  7:1864/1‑136 (MQ=255)                                           cccGTCACCGTCCACCTGCTGGACGAGGCCACGCGCCAGGCATGGAATGCCCCGCACGGTTATCACTTTCGCAGCAAGCATGTGCTGTTGCGCCAGGTACTGCAGCAACACCCGCTGGCAGTGCTGATcgacaccg                                                                                            <  2:120055/136‑1 (MQ=255)                                             cGTCACCGTCCACCTGCTGGACGAGGCCACGCGCCAGGCATGGAATGCCCCGCACGGTTATCACTTTCGCAGCAAGCATGTGCTGTTGCGCCAGGTACTGCAGCAACACCCGCTGGCAGTGCTGATcgacaccgac                                                                                          <  4:305970/136‑1 (MQ=255)                                             cGTCACCGTCCACCTGCTGGACGAGGCCACGCGCCAGGCATGGAATGCCCCGCACGGTTATCACTTTCGCAGCAAGCATGTGCTGTTGCGCCAGGTACTGCAGCAACACCCGCTGGCAGTGCTGATcgacaccgac                                                                                          <  4:102220/136‑1 (MQ=255)                                              gTCACCGTCCACCTGCTGGACGAGGCCACGCGCCAGGCATGGAATGCCCCGCACGGTTATCACTTTCGCAGCAAGCATGTGCTGTTGCGCCAGGTACTGCAGCAACACCCGCTGGCAGTGCTGATcgacaccgaca                                                                                         >  1:266660/1‑136 (MQ=255)                                                   cGTCCACCTGCTGGACGAGGCCACGCGCCAGGCATGGAATGCCCCGCACGGTTATCACTTTCGCAGCAAGCATGTGCTGTTGCGCCAGGTACTGCAGCAACACCCGCTGGCAGTGCTGATCGACACCGACACcttc                                                                                    <  7:159942/136‑1 (MQ=255)                                                       cACCTGCTGGACGAGGCCACGCGCCAGGCATGGAATGCCCCGCACGGTTATCACTTTCGCAGCAAGCATGTGCTGTTGCGCCAGGTACTGCAGCAACACCCGCTGGCAGTGCTGATCGACACCGACACCTTCTTcc                                                                                <  8:6715/136‑1 (MQ=255)                                                        aCCTGCTGGACGAGGCCACGCGCCAGGCATGGAATGCCCCGCACGGTTATCACTTTCGCAGCAAGCATGTGCTGTTGCGCCAGGTACTGCAGCAACACCCGCTGGCAGTGCTGATCGACACCGACACCTTCTTCCg                                                                               >  3:206778/1‑136 (MQ=255)                                                        aCCTGCTGGACGAGGCCACGCGCCAGGCATGGAATGCCCCGCACGGTTATCACTTTCGCAGCAAGCATGTGCTGTTGCGCCAGGTACTGCAGCAACACCCGCTGGCAGTGCTGATCGACACCGACACCTTCTTCCg                                                                               >  4:37488/1‑136 (MQ=255)                                                             ctgGACGAGGCCACGCGCCAGGCATGGAATGCCCCGCACGGTTATCACTTTCGCAGCAAGCATGTGCTGTTGCGCCAGGTACTGCAGCAACACCCGCTGGCAGTGCTGATCGACACCGACACCTTCTTCCGCAc                                                                            <  4:37201/134‑1 (MQ=255)                                                                   gAGGCCACGCGCCAGGCATGGAATGCCCCGCACGGTTATCACTTTCGCAGCAAGCATGTGCTGTTGCGCCAGGTACTGCAGCAACACCCGCTGGCAGTGCTGATCGACACCGACACCTTCTTCCGCACTTCGCCGt                                                                    >  1:289697/1‑136 (MQ=255)                                                                      gCCACGCGCCAGGCATGGAATGCCCCGCACGGTTATCACTTTCGCAGCAAGCATGTGCTGTTGCGCCAGGTACTGCAGCAACACCCGCTGGCAGTGCTGATCGACACCGACACCTTCTTCCGCACTTCGCCGTTg                                                                  <  6:88383/135‑1 (MQ=255)                                                                         aCGCGCCAGGCATGGAATGCCCCGCACGGTTATCACTTTCGCAGCAAGCATGTGCTGTTGCGCTAGGTACTGCAGCAACACCCGCTGGCAGTGCTGATCGACACCGACACCTTCTTCCGCACTTCGCCGTTGCATc                                                              >  8:175894/1‑136 (MQ=255)                                                                                               cGCACGGTTATCACTTTCGCAGCAAGCATGTGCTGTTGCGCCAGGTACTGCAGCACCACCCGCTGGCAGTGCTGATCGACACCGACACCTTCTTCCGCACTTCGCCGTTGCATCTGTTCGCCCGCGTGGCGCCCaa                                        >  7:214212/1‑136 (MQ=255)                                                                                                   cGGTTATCACTTTCGCAGCAAGCATGTGCTGTTGCGCTAGGTACTGCAGCAACACCCGCTGGCAGTGCTGATCGACACCGACACCTTCTTCCGCACTTCGCCGTTGCATCTGTTCGCCCGCGTGGCGCCCAACCGc                                    <  4:214174/136‑1 (MQ=255)                                                                                                   cGGTTATCACTTTCGCAGCAAGCATGTGCTGTTGCGCCAGGTACTGCAGCAACACCCGCTGGCAGTGCTGATCGACACCGACACCTTCTTCCGCACTTCGCCGTTGCATCTGTTCGCCCGCGTGGCGCCCAACCGc                                    <  6:22730/136‑1 (MQ=255)                                                                                                   cGGTTATCACTTTCGCAGCAAGCATGTGCTGTTGCGCCAGGTACTGCAGCAACACCCGCTGGCAGTGCTGATCGACACCGACACCTTCTTCCGCACTTCGCCGTTGCATCTGTTCGCCCGCGTGGCGCCCAACCGc                                    <  7:303525/136‑1 (MQ=255)                                                                                                   cGGTTATCACTTTCGCAGCAAGCATGTGCTGTTGCGCCAGGTACTGCAGCAACACCCGCTGGCAGTGCTGATCGACACCGACACCTTCTTCCGCACTTCGCCGTTGCATCTGTTCGCCCGCGTGGCGCCCAACCGc                                    <  5:82327/136‑1 (MQ=255)                                                                                                    ggTTATCACTTTCGCAGCAAGCATGTGCTGTTGCGCCAGGTACTGCAGCAACACCCGCTGGCAGTGCTGATCGACACCGACACCTTCTTCCGCACTTCGCCGTTGCATCTGTTCGCCCGCGTGGCGCCCAACCGcc                                   >  4:179907/1‑136 (MQ=255)                                                                                                         tCACTTTCGCAGCAAGCATGTGCTGTTGCGCCAGGTACTGCAGCAACACCCGCTGGCAGTGCTGATCGACACCGACACCTTCTTCCGCACTTCGCCGTTGCATCTGTTCGCCCGCGTGGCGCCCAACCGCctgctg                              >  7:171491/1‑136 (MQ=255)                                                                                                           aCTTTCGCAGCAAGCATGTGCTGTTGCGCTAGGTACTGCAGCAACACCCGCTGGCAGTGCTGATCGACACCGACACCTTCTTCCGCACTTCGCCGTTGCATCTGTTCGCCCGCGTGGCGCCCAACCGCCTGCtgt                             <  1:13300/135‑1 (MQ=255)                                                                                                           aCTTTCGCAGCAAGCATGTGCTGTTGCGCCAGGTACTGCAGCAACACCCGCTGGCAGTGCTGATCGACACCGACACCTTCTTCCGCACTTCGCCGTTGCATCTGTTCGCCCGCGTGGCGCCCAACCGCCTGCtgtg                            <  7:76833/136‑1 (MQ=255)                                                                                                           aCTTTCGCAGCAAGCATGTGCTGTTGCGCCAGGTACTGCAGCAACACCCGCTGGCAGTGCTGATCGACACCGACACCTTCTTCCGCACTTCGCCGTTGCATCTGTTCGCCCGCGTGGCGCCCAACCGCCTGCtgt                             <  5:266761/135‑1 (MQ=255)                                                                                                              ttCGCAGCAAGCATGTGCTGTTGCGCCAGGTACTGCAGCAACACCCGCTGGCAGTGCTGATCGACACCGACACCTTCTTCCGCACTTCGCCGTTGCATCTGTTCGCCCGCGTGGCGCCCAACCGCCTGCTGTGCaa                         >  7:153947/1‑136 (MQ=255)                                                                                                                 gcagcaAGCATGTGCTGTTGCGCCAGGTACTGCAGCAACACCCGCTGGCAGTGCTGATCGACACCGACACCTTCTTCCGCACTTCGCCGTTGCATCTGTTCGCGCGCGTGGCGCCCAACCGCCTGCTGTGCAACGc                      >  3:164292/1‑136 (MQ=255)                                                                                                                   agcaagcaTGTGCTGTTGCGCCAGGTACTGCAGCAACACCCGCTGGCAGTGCTGATCGACACCGACACCTTCTTCCGCACTTCGCCGTTGCATCTGTTCGCCCGCGTGGCGCCCAACCGCCTGCTGTGCTAGGCCa                    >  8:240081/1‑136 (MQ=255)                                                                                                                   agcaagcaTGTGCTGTTGCGCCAGGTACTGCAGCAACACCCGCTGGCAGTGCTGATCGACACCGACACCTTCTTCCGCACTTCGCCGTTGCATCTGTTCGCCCGCGTGGCGCCCAACCGCCTGCTGTGCAACGCCa                    >  5:208511/1‑136 (MQ=255)                                                                                                                   agcaagcaTGTGCTGTTGCGCCAGGTACTGCAGCAACACCCGCTGGCAGTGCTGATCGACACCGACACCTTCTTCCGCACTTCGCCGTTGCATCTCTTCGCCCGCGTGGCGCCCAACCGCCTGCTGTGCAACGCCa                    >  4:80681/1‑136 (MQ=255)                                                                                                                     caagcaTGTGCTGTTGCGCCAGGTACTGCAGCAACACCCGCTGGCAGTGCTGATCGACACCGACACCTTCTTCCGCACTTCGCCGTTGCATCTGTTCGCCCGCGTGGCGCCCAACCGCCTGCTGTGCAACGCCATc                  <  5:312223/136‑1 (MQ=255)                                                                                                                      aagcaTGTGCTGTTGCGCCAGGTACTGCAGCAACACCCGCTGGCAGTGCTGATCGACACCGACACCTTCTTCCGCACTTCGCCGTTGCATCTGTTCGCCCGCGTGGCGCCCAACCGCCTGCTGTGCAACGCCATCg                 <  3:36721/136‑1 (MQ=255)                                                                                                                      aagcaTGTGCTGTTGCGCCAGGTACTGCAGCAACACCCGCTGGCAGTGCTGATCGACACCGACACCTTCTTCCGCACTTCGCCGTTGCATCTGTTCGCCCGCGTGGCGCCCAACCGCCTGCTGTGCAACGCCATCg                 <  2:52785/136‑1 (MQ=255)                                                                                                                      aagcaTGTGCTGTTGCGCCAGGTACTGCAGCAACACCCGCTGGCAGTGCTGATCGACACCGACACCTTCTTCCGCACTTCGCCGTTGCATCTGTTCGCCCGCGTGGCGCCCAACCGCCTGCTGTGCAACGCCATCg                 <  2:282963/136‑1 (MQ=255)                                                                                                                      aagcaTGTGCTGTTGCGCCAGGTACTGCAGCAACACCCGCTGGCAGTGCTGATCGACACCGACACATTCTTCCGCACTTCGCCGTTGCATCTGTTCGCCCGCGTGGCGCCCAACCGCCTGCTGTGCAACGCCATCg                 <  6:51601/136‑1 (MQ=255)                                                                                                                             tgCTGTTGCGCCAGGTACTGCAGCAACACCCGCTGGCAGTGCTGATCGACACCGACACCTTCTTCCGCACTTCGCCGTTGCATCTGTTCGCCCGCGTGGCGCCCAACCGCCTGCTGTGCAACGCCATCGGCAcgcg          <  8:144451/136‑1 (MQ=255)                                                                                                                             tgCTGTTGCGCCAGGTACTGCAGCAACACCCGCTGGCAGTGCTGATCGACACCGACACCTTCTTCCGCACTTCGCCGTTGCATCTGTTCGCCCGCGTGGCGCCCAACCGCCTGCTGTGCAACGCCATCGGCAcgcg          <  1:4909/136‑1 (MQ=255)                                                                                                                                tGTTGCGCCAGGTACTGCAGCAACACCCGCTGGCAGTGCTGATCGACACCGACACCTTCTTCCGCACTTCGCCGTTGCATCTGTTCGCCCGCGTGGCGCCCAACCGCCTGCTGTGCAACGCCATCGGCACGCGCTa       >  7:45815/1‑136 (MQ=255)                                                                                                                                  ttGCGCCAGGTACTGCAGCAACACCCGCTGGCAGTGCTGATCGACACCGACACCTTCTTCCGCACTTCGCCGTTGCATCTGTTCGCCCGCGTGGCGCCCAACCGCCTGCTGTGCAACGCCATCGGCACGCGCTACg     <  6:22941/136‑1 (MQ=255)                                                                                                                                  ttGCGCCAGGTACTGCAGCAACACCCGCTGGCAGTGCTGATCGACACCGACACCTTCTTCCGCACTTCGCCGTTGCATCTGTTCGCCCGCGTGGCGCCCAACCGCCTGCTGTGCAACGCCATCGGCACGCGCTACg     >  3:290956/1‑136 (MQ=255)                                                                                                                                  ttGCGCCAGGTACTGCAGCAACACCCGCTGGCAGTGCTGATCGACACCGACACCTTCTTCCGCACTTCGCCGTTGCATCTGTTCGCCCGCGTGGCGCCCAACCGCCTGCTGTGCAACGCCATCGGCACGCGCTACg     <  3:286706/136‑1 (MQ=255)                                                                                                                                  ttGCGCCAGGTACTGCAGCAACACCCGCTGGCAGTGCTGATCGACACCGACACCTTCTTCCGCACTTCGCCGTTGCATCTGTTCGCCCGCGTGGCGCCCAACCGCCTGCTGTGCAACGCCATCGGCACGCGCTACg     <  1:133541/136‑1 (MQ=255)                                                                                                                                     cgcCAGGTACTGCAGCAACACCCGCTGGCAGTGCTGATCGACACCGACACCTTCTTCCGCACTTCGCCGTTGCATCTGTTCGCCCGCGTGGCGCCCAACCGCCTGCTGTGCAACGCCATCGGCACGCGCTACGgc   <  6:47946/135‑1 (MQ=255)                                                                                                                                     cgcCAGGTACTGCAGCAACACCCGCTGGCAGTGCTGATCGACACCGACACCTTCTTCCGAACTTCGCCGTTGCATCTGTTCGCCCGCGTGGCGCCCAACCGCCTGCTGTGCAACGCCATCGGCACGCGCTACGgcg  >  8:125834/1‑136 (MQ=255)                                                                                                                                        |                                                                                                                                     ATCCAGGTCTACACCGACAATGCGCAGCCATACGCCAGGCTGCCCGTCACCGTCCACCTGCTGGACGAGGCCACGCGCCAGGCATGGAATGCCCCGCACGGTTATCACTTTCGCAGCAAGCATGTGCTGTTGCGCCAGGTACTGCAGCAACACCCGCTGGCAGTGCTGATCGACACCGACACCTTCTTCCGCACTTCGCCGTTGCATCTGTTCGCCCGCGTGGCGCCCAACCGCCTGCTGTGCAACGCCATCGGCACGCGCTACGGCG  >  ACB143/5667828‑5668095

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑