Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 5,693,153 T→G 34.3% R228R (CGT→CGG)  pyrC' → Dihydroorotase‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1435,693,1530TG34.3% 13.6 / 4.5 31R228R (CGT→CGG) pyrC'Dihydroorotase‑like protein
Reads supporting (aligned to +/- strand):  ref base T (8/12);  new base G (6/5);  total (14/17)
Fisher's exact test for biased strand distribution p-value = 4.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

GCCCAGGGCGGCCTGGCCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGG‑CCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTC  >  ACB143/5693028‑5693287
                                                                                                                             |                                                                                                                                       
gCCCAGGGCGGCCTGGCCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTc                                                                                                                                <  5:224005/135‑1 (MQ=255)
   cAGGGCGGCCTGGCCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCCGc                                                                                                                             >  8:222906/1‑135 (MQ=255)
         ggcCTGGCCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTAAGCCAGATcc                                                                                                                      >  7:210282/1‑135 (MQ=255)
         ggcCTGGCCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGGGCGCCCTTCAGCCCGATCa                                                                                                                      >  1:33724/1‑136 (MQ=255)
             tGGCCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAATCCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGGGGGGGCCCTTCAGCCCGATCAcccg                                                                                                                  >  2:93087/1‑134 (MQ=255)
                       aGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTCGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGGGCGGGGGGCCGTGAGCCACGACACCCGCGCCCGGGgc                                                                                                        >  5:281654/1‑136 (MQ=255)
                         gTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCTAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGgcg                                                                                                       <  7:271037/135‑1 (MQ=255)
                             cATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGGGCCCTTCAGCCCGATAACCAGCGCCCCTGGCGCGCgg                                                                                                  >  8:271312/1‑136 (MQ=255)
                               tGGCCCGCTCCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGGGGCCCTGGCGCGCAACCTGCTGCGGGGGGACCAGCGCGGCGTGCGTGCGCACTTCAGCCACATCACCAGCGCCCGTGGCGCGCGGCt                                                                                                <  5:72742/136‑1 (MQ=255)
                               gggCCTGCTTCCTCGGCCTGCCGGGCAACCCGGCGCGCCCCGAAACCGGGCCCCTGGCGCGCAACCTGCTGCGGGTGGAACTCTGCGGCGTGCGTGCGCACTTCCGCCAGATCACCCGCGCCCGGGGCGCGCGGCt                                                                                                <  2:178455/135‑1 (MQ=255)
                                   cAGCTTCCTCGGCCTGCCGGGCAGCCCGGAGAGCGCGGAAACCGTGGCCCTGCCGCGCAACCTGCTGCGGGTGGAACAGTGCGGGGTGCGGGCGCACTTCAGCCAGATCCCCAGCGCCCGGGGCGCGCGGCTGATc                                                                                            <  1:246852/136‑1 (MQ=255)
                                    cgCTGCCTGGCCCGGCCGGGCACCCCGCCGAGCGCCTAAACCGGGGCCCTGGGGCGAAACCGGCTGCGGGGGCAACATAGCGGGGGGCGGGCGCACTACCGCCCTACCACCCGCGCCCGTGGCGCGCGGCTGATCg                                                                                           <  4:131217/135‑1 (MQ=255)
                                    aGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCCCGTAAGGCAGATCACCAGCGCCCGTGGCGCGCGGCTGCTCg                                                                                           >  5:250253/1‑136 (MQ=255)
                                         ccTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCGGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCgcccag                                                                                      <  3:199148/136‑1 (MQ=255)
                                          cTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCCGCGCCCGTGGCGCGCGGCTGATCgcccagg                                                                                     <  5:249729/136‑1 (MQ=255)
                                          cTCGGCCGGCCGGGCATCCGGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCATATCACCAGCGCCCGTGGCGCGCGGCTGATCgcccagg                                                                                     <  1:12616/136‑1 (MQ=255)
                                              gCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGGGCGGACTTCAGCCCGATCACCAGCGCCCGGGGCGCGCGGCTGATCgccccgg‑cccc                                                                                >  8:303671/1‑135 (MQ=255)
                                                            ccGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGGGGGCCCTTCAGCCAGATAACCCCCGCCCGTGGCGCGCGGCTGATCGCCCCGG‑CCCCGGCCGTGGGCCGGc                                                                  >  1:60833/1‑136 (MQ=255)
                                                             cGGCGAGCGCCGAAACCGTGGCCCTGGCGCGAAACCTGCTGCTGGTGGAACAGAGCGGCGTGGGTGCGCACTTCAGCCAGATCACCAGCGCCGGTGGCGCGCGGCTGATCGCCCAGG‑CCCAGGCCTTGGGCCTGcc                                                                 <  2:198054/136‑1 (MQ=255)
                                                                   gcgcCTAAACGGTGGCCGTGGCGCGCAACGTGGTGCTGGTGTAACAGAGCGGGGTGCGTGCGCACTTCCGCCAGATCACCCGCGCCCGTGGCGCGCGGCTGATCGCCCAGG‑CCCAGGCCTTGGGCCTGCCGGTAAc                                                           <  3:132783/136‑1 (MQ=255)
                                                                   gcgcCGAAACCGTGGCCCGGGCGCGCAACCTGCTGCTGGTGCAACAGCGCGGCGTGCGGGCGCACTTCCGCCAGACCCCACGCGCCCGGGGCGCGCGGCTGATCGCCCAGG‑CCCAGGCCTTGGGCCTGCCGGTAAc                                                           <  1:40490/136‑1 (MQ=255)
                                                                       cGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCCATTGAGCCAGATACCCCGCGCCCCGGGCGCGCGGCTGATCGCCCCGGCCCCAGGCC‑TGGGGCTGCCGGGAAccgcc                                                       >  8:209830/1‑136 (MQ=255)
                                                                        gAAACCGTGGCCCGGGCGCGCAACCTGCGGCTGGTGCAACAGAGCGGCGTGCGGGCGCACTTCAGCCAGAACACCCGCGCCGGTGGCGCGCGGCTGATCGCCCAGG‑CCCAGGCCTTGGGCCTGCCGGTAAccgccg                                                      <  5:168995/136‑1 (MQ=255)
                                                                             ggTGGCCCTGGGGGGCAACCTGCGGGTGGTGTAACAGAGGGGGGTGCTTGGGCACTTCAGCCAGATCACCAGCGCCCGTGGGGCGCGGCTGATCGCCCAGG‑CTCAGTAATTGGGCCTGCCGGTAACCGCCGATGTa                                                 <  8:23965/135‑1 (MQ=255)
                                                                                         gcgcAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCCCTTCAGCCAGCTACCCCGCGCCCGTGGCGCGCGGCTGATCGCCCCCG‑CCCCGGCCGTGGGGCTGCCGGTAACCGCCGATGTAGCGCTGTACCAg                                     >  1:26804/1‑136 (MQ=255)
                                                                                          cgcAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCCCTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCCGG‑CCCCGGCCGTGGGCCAGCCGGTTACCGCCGCTGTAGCGCTGTACCCGc                                    >  6:59934/1‑136 (MQ=255)
                                                                                                ctgcggctgGTGGAACAGAGGGGGGGGCGTGCGCACTTCAGCCAGACCACCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGG‑CCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATcc                              <  3:289432/136‑1 (MQ=255)
                                                                                                                   gcggcgTGCGGGCGCACTTCCCCCAGATCCCCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGG‑CCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTgcg           <  2:222178/136‑1 (MQ=255)
                                                                                                                    cggcgTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCgcccagg                                                                                     >  8:84244/1‑62 (MQ=255)
                                                                                                                    cggcgTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGGGGCGCGCGGCTGATCgcccagg                                                                                     <  7:84244/62‑1 (MQ=255)
                                                                                                                     ggcgTGGGGGCGCACATCAGCCCGATCACCACCGCCCCTGGGGCGCGGCGGCTAGCCCCCG‑CCCACGCCGTGGGGCGGCCGGGTACCGCCGATGTAGCGCTGTACCACCTGATACGCACCGCCCAGTCGCGGcgcg         >  8:21910/1‑136 (MQ=255)
                                                                                                                      gcgtgcgtGCGCACTTCAGCCACATAACCAGCGCCCGTGGCGCGCGGCTGCTACCCCAGG‑CCCAGGCCATTGGCCTGCCGGTAACCGCCGATGTTGCGCAGTACCAGCAGATACTCACCGACCAGTAGCAgcgc          >  6:133403/1‑134 (MQ=255)
                                                                                                                        gggcgtgcgCACTTCCGCCACAACACCCGCGCCCGTGGCGCGCGGCTGATCGCCCAGG‑CCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt      <  1:289330/134‑1 (MQ=255)
                                                                                                                        gggcgggcgCACTCCCCCCAGAGCACCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGG‑CCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt      <  3:307708/134‑1 (MQ=255)
                                                                                                                            gtgcgCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCCGG‑CCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCAGTACCAGCTGATCCTCACGGCCGAGTCGCTGCGCGCGGtctc  >  7:274257/1‑136 (MQ=255)
                                                                                                                             |                                                                                                                                       
GCCCAGGGCGGCCTGGCCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGG‑CCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTC  >  ACB143/5693028‑5693287

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑