Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB143	844,922	C→T	6.2%	A217A (GCC→GCT)	hemA →	Glutamyl‑tRNA reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB143	844,922	0	C	T	6.2%	214.6 / 3.5	64	A217A (GCC→GCT)	hemA	Glutamyl‑tRNA reductase
Reads supporting (aligned to +/- strand):  ref base C (29/31);  new base T (2/2);  total (31/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGATTTTCGCCGACCTGGGCCGCAGCCAGGCCCTGCTGATCGGTGCCGGCGAAACCATCACCCTGGTCGCCCGCCACCTGCACGAGCAGGGCGTACGCCGTATCGTGGTGGCCAACCGCACCCTGGAGCGCGCCAGCATCCTGGCCGAGCAGTTCGGCGCGCATGCCGTGCTGCTGGCAGACATCCCCCAGGAACTGGCCAACAGTGACATCGTGATCAGTTCCACCGCCAGCCAGTTGCCGATTCTGGGCAAGGGCGCGGTCGAGAG  >  ACB143/844789‑845056                                                                                                                                      |                                                                                                                                       aGATTTTCGCCGACCTGGGGCGCAGCCAGGCCCTGCTGATCGGTGCCGGCGAAACCATCACCCTGGTCGCCCGCCACCTGCACGAGCAGGGCGTACGCCGTATCGTGGTGGCCAACCGCACCCTGGAGCGCGCCAg                                                                                                                                      <  1:205801/136‑1 (MQ=255) aGATTTTCGCCGACCTGGGCCGCAGCCAGGCCCTGCTGATCGGTGCCTGCGAAACCATCACCCTGGTCGCCCGCCACCTGCACGAGCAGGGCGTACGCCGTATCGTGGTGGCCAACCGCACCCTGGAGCGCGCCAg                                                                                                                                      <  2:165019/136‑1 (MQ=255) aGATTTTCGCCGACCTGGGCCGCAGCCAGGCCCTGCTGATCGGTGCCGGCGAAACCATCACCCTGGTCGCCCGCCACCTGCACGAGCAGGGCGTACGCCGTATCGTGGTGGCCAACCGCACCCTGGAGCGCGCCAg                                                                                                                                      <  8:255648/136‑1 (MQ=255) aGATTTTCGCCGACCTGGGCCGCAGCCAGGCCCTGCTGATCGGTGCCGGCGAAACCATCACCCTGGTCGCCCGCCACCTGCACGAGCAGGGCGTACGCCGTATCGTGGTGGCCAACCGCACCCTGGAGCGCGCCAg                                                                                                                                      >  8:172917/1‑136 (MQ=255)   aTTTTCGCCGACCTGGGCCGCAGCCAGGCCCTGCTGATCGGTGCCGGCGACACCATCCCCCTGGTCGCCCGCCACCTGCACGAGCAGGGCGTACGCCGTATCGTGGTGGCCAACCGCACCCTGGAGCGCGCCAGCa                                                                                                                                    >  1:248111/1‑136 (MQ=255)   aTTTTCGCCGACCTGGGCCGCAGCCAGGCCCTGCTGATCGGTGCCGGCGAAACCATCACCCTGGTCGCCCGCCACCTGCACGAGCAGGGCGTACGCCGTATCGTGGTGGCCAACCGCACCCTGGAGCGCGCCAGCa                                                                                                                                    >  5:209398/1‑136 (MQ=255)       tCGCCGACCTGGGCCGCAGCCAGGCCCTGCTGATCGGTGCCGGCGAAACCATCACCCTGGTCGCCCGCCACCTGCACGAGCAGGGCGTACGCCGTATCGTGGTGGCCAACCGCACCCTGGAGCGCGCCAGCATcc                                                                                                                                 <  5:43456/135‑1 (MQ=255)       tCGCCGACCTGGGCCGCAGCCAGGCCCTGCTGATCGGTGCCGGCGAAACCATCACCCTGGTCGCCCGCCACCTGCACGAGCAGGGCGTACGCCGTATCGTGGTGGCCAACCGCACCCTGGAGCGCGCCAGCATCCt                                                                                                                                >  7:92710/1‑136 (MQ=255)         gCCGACCTGGGCCGCAGCCAGGCCCTGCTGATCGGTGCCGGCGAAACCATCACCCTGGTCGCCCGCCACCTGCACGAGCAGGGCGTACGCCGTATCGTGGTGGCCAACCGCACCCTGGAGCGCGCCAGCATCCTg                                                                                                                               <  5:216455/135‑1 (MQ=255)                  ggCCGCAGCCAGGCCCTGCTGATCGGTGCCGGCGAAACCATCACCCTGGTCGCCCGCCACCTGCACGAGCAGGGCGTACGCCGTATCGTGGTGGCCAACCGCACCCTGGAGCGCGCCAGCATCCTGGCCGAGCAg                                                                                                                      <  8:293614/135‑1 (MQ=255)                          ccAGGCCCTGCTGATCGGTGCCGGCGAAACCATCACCCTGGTCGCCCGCCACCTGCACGAGCAGGGCGTACGCCGTATCGTGGTGGCCAACCGCACCCTGGAGCGCGCCAGCATCCTGGCCGAGCAGTTCGgcgcg                                                                                                             >  5:134069/1‑136 (MQ=255)                                     tgATCGGTGCCGGCGAAACCATCACCCTGGTCGCCCGCCACCTGCACGAGCAGGGCGTACGCCGTATCGTGGTGGCCAACCGCACCCTGGAGCGCGCCAGCATCCTGGCCGAGCAGTTCGGCGCGCATGCCGtgct                                                                                                  >  4:292548/1‑136 (MQ=255)                                     tgATCGGTGCCGGCGAAACCATCACCCTGGTCGCCCGCCACCTACACGAGCAGGGCGTACGCCGTATCGTGGTGGCCAACCGCACCCTGGAGCGCGCCAGCATCCTGGCCGAGCAGTTCGGCGCGCATGCCGtgct                                                                                                  >  8:260664/1‑136 (MQ=255)                                       aTCGGTGCCGGCGAACCCATCACCCTGGTCGCCCGCCACCTGCACGAGCAGGGCGTACGCCGTATCGTGGTGGCCAACCGCACCCTGGAGCGCGCCAGCATCCTGGCCGAGCAGTTCGGCGCGCATGCCGtgctgc                                                                                                >  4:137921/1‑136 (MQ=255)                                           gTGCCGGCGAAACCATCACCCTGGTCGCCCGCCACCTGCACGAGCAGGGCGTACGCCGTATCGTGGTGGCCAACCGCACCCTGGAGCGCGCCAGCATCCTGGCCGAGCAGTTCGGCGCGCATGCCGTGCTGCTGGc                                                                                            <  8:30944/136‑1 (MQ=255)                                              ccGGCGAAACCATCACCCTGGTCGCGCGCCACCTGCACGAGCAGGGCGTACGCCGTATCGTGGTGGCCTACCGCACCCTGGAGCGCGCTAGCATCCTGGCCGAGCAGTTCGGCGCGCTTGCCGTGCTGCTGGCAGa                                                                                         <  7:163668/136‑1 (MQ=255)                                              ccGGCGAAACCATCACCCTGGTCGCCCGCCACCTGCACGAGCAGGGCGTACGCCGTATCGTGGTGGCCTACCGCACCCTGGAGCGCGCTAGCATCCTGGCCGAGCAGTTCGGCGCGCATGCCGTGCTGCTGGCAGa                                                                                         <  7:163669/136‑1 (MQ=255)                                              ccGGCGAAACCATCACCCTGGTCGCCCGCCACCTGCACGAGCAGGGCGTACGCCGTATCGTGGTGGCCAACCGCACCCTGGAGCGCGCCAGCATCCTGGCCGAGCAGTTCGGCGCGCATGCCGTGCTGCTGGCAGa                                                                                         <  6:213419/136‑1 (MQ=255)                                              ccGGCGAAACCATCACCCTGGTCGCCCGCCACCTGCACGAGCAGGGCGTACGCCGTATCGTGGTGGCCAACCGCACCCTGGAGCGCGCCAGCATCCTGGCCGAGCAGTTCGGCGCGCATGCCGTGCTGCTGGCAGa                                                                                         <  1:51316/136‑1 (MQ=255)                                              ccGGCGAAACCATCACCCTGGTCGCCCGCCACCTGCACGAGCAGGGCGTACGCCGTATCGTGGTGGCCAACCGCACCCTGGAGCGCGCCAGCATCCTGGCCGAGCAGTTCGGCGCGCATGCCGTGCTGCTGGCAGa                                                                                         <  7:266686/136‑1 (MQ=255)                                               cGGCGAAACCATCACCCTGGTCGCCCGCCACCTGCACGAGCAGGGCGTACGCCGTATCGTGGTGGCCAACCGCACCCTGGAGCGCGCCAGCATCCTGGCCGAGCAGTTCGGCGCGCATGCCGTGCTGCTGGCAGa                                                                                         >  8:133587/1‑135 (MQ=255)                                               cGGCGAAACCATCACCCTGGTCGCCCGCCACCTGCACGAGCAGGGCGTACGCCGTATCGTGGTGGCCAACCGCACCCTGGAGCGCGCCAGCATCCTGGCCGAGCAGTTCGGCGCGCATGCCGTGCTGCTGGCAGAc                                                                                        <  1:64871/136‑1 (MQ=255)                                                    aaaCCATCACCCTGGTCGCCCGCCACCTGCACGAGCAGGGCGTACGCCGTATCGTGGTGGCCAACCGCACCCTGGAGCGCGCCAGCATCCTGGCCGCGCAGTTCGGCGCGCATGCCGTGCTCCTGGCAGACATccc                                                                                   >  7:163922/1‑136 (MQ=255)                                                         aTCACCCTGGTCGCCCGCCCCCTGCACGAGCAGGGCGTACGCCGTATCGTGGTGGCCAACCGCACCCTGGAGCGCGCCAGCATCCTGGCCGAGCAGTTCGGCGCGCATGCCGTGCTGCTGGCAGACATCCCCCAg                                                                               >  5:34231/1‑135 (MQ=255)                                                         aTCACCCTGGTCGCCCGCCACCTGCACGAGCAGGGCGTACGCCGTATCGTGGTGGCCAACCGCACCCTGGAGCGCGCTAGCATCCTCGCCGAGCAGTTCGGCGCGCATGCCGTGCAGCTGGCAGACATCCCCCAg                                                                               >  8:163668/1‑135 (MQ=255)                                                         aTCACCCTGGTCGCCCGCCACCTGCACGAGCAGGGCGTACGCCGTATCGTGGTGGCCAACCGCACCCTGGAGCGCGCCAGCATCCTGGCCGAGCAGTTCGGCGCGCATGCCGTGCTGCTGGCAGCCATCCCCCAg                                                                               >  4:10800/1‑135 (MQ=255)                                                         aTCACCCTGGTCGCCCGCCACCTGCACGAGCAGGGCGTACGCCGTATCGTGGTGGCCAACCGCACCCTGGAGCGCGCCAGCATCCTGGCCGAGCAGTTCGGCGCGCATGCCGTGCTGCTGGCAGACATCCCCCAg                                                                               >  2:206832/1‑135 (MQ=255)                                                         aTCACCCTGGTCGCCCGCCACCTGCACGAGCAGGGCGTACGCCGTATCGTGGTGGCCAACCGCACACTGGAGCGCGCTAGCATCCTGGCAGAGCAGTTCGGCGCGCATTCCGTGAAGCTGGCAGACATCCACCAg                                                                               >  8:163669/1‑135 (MQ=255)                                                                  gTCGCCCGCCACCTGCACGAGCAGGGCGTACGCCGTATCGTGGTGGCCAACCGCACCCTGGAGCGCGCCAGCATCCTGGCCGAGCAGTTCGGCGCGCATGCCGTGCTGCTGGCAGACATCCCCCAGGAACTGGc                                                                       <  6:13637/134‑1 (MQ=255)                                                                    cgcccgccACCTGCACGAGCAGGGCGTACGCCGTATCGTGGTGGCCAACCGCACCCTGGAGCGCGCCAGCATCCTGGCCGAGCAGTTCGGCGCGCATGCCGTGCTGCTGGCAGACATCCCCCAGGAACTGGCCaa                                                                    >  4:298720/1‑135 (MQ=255)                                                                     gcccgccACCTGCACGAGCAGGGCGTACGCCGTATCGTGGTGGCCAACCGCACCCTGGAGCGCGCCAGCCTCCTGGCCGAGCAGTTCGGCGCGCATGCCGTGCTGCTGGCAGACATCCCCCAGGAACTGGCCaa                                                                    >  1:214026/1‑134 (MQ=255)                                                                     gcccgccACCTGCACGAGCAGGGCGTACGCCGTATCGTGGTGGCCAACCGCACCCTGGAGCGCGCCAGCATCCTGGCCGAGCAGTTCGGCGCGCATGCCGTGCTGCTGGCAGACATCCCCCAGGAACTGGCCaa                                                                    >  2:11013/1‑134 (MQ=255)                                                                     gcccgccACCTGCACGAGCAGGGCGTACGCCGTATCGTGGTGGCCAACCGCACCCTGGAGCGCGCCAGCATCCTGGCCGAGCAGTTCGGCGCGCATGCCGTGCTGCTGGCAGACATCCCCCAGGAACTGGCCaa                                                                    >  4:205968/1‑134 (MQ=255)                                                                             ccTGCACGAGCAGGGCGTACGCCGTATCGTGGTGGCCAACCGCACCCTGGAGCGCGCCAGCATCCTGGCCGAGCAGTTCGGCGCGCATGCCGTGCTGCTGGCAGACATCCCCCAGGAACTGGCCAACAGTGACATc                                                          <  1:108337/136‑1 (MQ=255)                                                                                 cACGATCAGTGCGTACGCCGTATCGTGGTGGCCAACCGCACCGTGGAGCGCGCCAGCATCCTGGCCGAGCAGTTCGGCGCGCATGCCGTGCTGCTGGCAGACATCCCCCAGGAACTGGCCAACAGTGACATCGt                                                        <  8:283172/134‑1 (MQ=255)                                                                                 cACGAGCAGGGCGTACGCCGTATCGTGGTGGCCAACCGCACCCTGGAGCGCGCCAGCATCCTGGCCGAGCAGTTCGGCGCGCATGCCGTGCTGCTGGCAGACATCCCCCAGGAACTGGCCAACAGTGACATCGt                                                        <  3:294275/134‑1 (MQ=255)                                                                                 cACGAGCAGGGCGTACGCCGTATCGTGGTGGCCAACCGCACCCTGGAGCGCGCCAGCATCCTGGCCGAGCAGTTCGGCGCGCATGCCGTGCTGCTGGCAGACATCCCCCAGGAACTGGCCAACAGTGACATCGt                                                        <  3:152008/134‑1 (MQ=255)                                                                                 cACGAGCAGGGCGTACGCCGTATCGTGGTGGCCAACCGCACCCTGGAGCGCGCCAGCATCCTGGCCGAGCAGTTCGGCGCGCATGCCGTGCTGCTGGCAGACATCCCCCAGGAACTGGCCAACAGTGACATCGt                                                        <  1:112281/134‑1 (MQ=255)                                                                                 cACGAGCAGGGCGTACGCCGTATCGTGGTGGCCAACCGCACCCTGGAGCGCGCCAGCATCCTGGCCGAGCAGTTCGGCGCGCATGCCGTGCTGCTGGCAGACATCCCCCAGGAACTGGCCAACAGTGACATCGt                                                        <  2:259021/134‑1 (MQ=255)                                                                                 cACGAGCAGGGCGTACGCCGTATCGTGGTGGCCAACCGCACCCTGGAGCGCGCCAGCACCCTGGCCGAGCAGTTCGGCGCGCATGCCGTGCTGCTGGCAGACATCCCCCAGGAACTGGCCAACAGTGACATCGt                                                        <  4:92281/134‑1 (MQ=255)                                                                                    gAGCAGGGCGTACGCCGTATCGTGGTGGCCAACCGCACCCTGGAGCGCGCCAGCATCCTGGCCGAGCAGTTCGGCGCGCATGCCGTGCTGCTGGCAGACATCCCCCAGGAACTGGCCAACAGTGACATCGTGAt                                                     >  2:265560/1‑134 (MQ=255)                                                                                    gAGCAGGGCGTACGCCGTATCGTGGTGGCCAACCGCACCCTGGAGCGCGCCAGCATCCTGGCCGAGCAGTTCGGCGCGCATGCCGTGCTGCTGGCAGACATCCCCCAGGAACTGGCCAACAGTGACATCGTGAt                                                     >  1:22287/1‑134 (MQ=255)                                                                                              tACGCCGTATCGTGGTGGCCAACCGCACCCTGGAGCGCGCCAGCATCCTGGCCGAGCAGTTCGGCGCGCATGCCGTGCTGCTGGCAGACATCCCCCAGGAACTGGCCAACAGTGACATCGTGATCAGTTCCACCgc                                         >  2:271469/1‑136 (MQ=255)                                                                                                   cGTATCGTGGTGGCCAACCGCACCCTGGAGCGCGCCAGCATCCTGGCCGAGCAGTTCGGCGCGCATGCCGTGCTGCTGGCAGACATCCCCCAGGAACTGGCCAACAGTGACATCGTGATCAGTTCCACCgccagc                                     >  7:45763/1‑135 (MQ=255)                                                                                                       tCGTGGTGGCCAACCGCACCCTGGAGCGCGCCAGCATCCTGGCCGAGCAGTTCGGCGCGCATGCCGTGCTGCTGGCAGACATCCCCCAGGAACTGGCCAACAGTGACATCGTGATCAGTTCCACCGCCAGCCAGtt                                >  2:59511/1‑136 (MQ=255)                                                                                                       tCGTGGTGGCCAACCGCACCCTGGAGCGCGCCAGCATCCTGGCCGAGCAGTTCGGCGCGCATGCCGTGCTGCTGGCAGACATCCCCCAGGAACTGGCCAACAGTGACATCGTGATCAGTTCCACCGCCAGCCAGt                                 <  7:247810/135‑1 (MQ=255)                                                                                                        cGTGGTGGCCAACCGCACCCTGGAGCGCGCCAGCATCCTGGCCGAGCAGTTCGGCGCGCATGCCGTGCTGCTGGCAGACATCCCCCAGGAACTGGCCAACAGTGACATCGTGATCAGTTCCACCGCCAGCCAGTTg                               >  2:89332/1‑136 (MQ=255)                                                                                                        cGTGGTGGCCAACCGCACCCTGGAGCGCGCCAGCATCCTGGCCGAGCAGTTCGGCGCGCATGCCGTGCTGCTGGCAGACATCCCCCAGGAACTGGCCAACAGTGACATCGTGATCAGTTCCACCGCCAGCCAGTTg                               >  6:220196/1‑136 (MQ=255)                                                                                                         gtggtgGCCAACCGCACCCTGGAGCGCGCCAGCATCCTGGCCGAGCAGTTCGGCGCGCATGCCGTGCTGCTGGCAGACATCCCCCAGGAACTGGCCAACAGTGACATCGTGATCAGTTCCACCGCCAGCCAGTTGc                              >  6:190304/1‑136 (MQ=255)                                                                                                            gtggCCAACCGCACCCTGGAGCGCGCCAGCATCCTGGCCGAGCAGTTCGGCGCGCATGCCGTGCTGCTGGCAGACATCCCCCAGGAACTGGCCAACAGTGACATCGTGATCAGTTCCACCGCCAGCCAGTTGCCg                            <  7:260664/135‑1 (MQ=255)                                                                                                             tggCCAACCGCACCCTGGAGCGCGCCAGCATCCTGGCCGAGCAGTTCGGCGCGCATGCCGTGCTGCTGGCAGACATCCCCCAGGAACTGGCCAACAGTGACATCGTGATCAGTTCCACCGCCAGCCAGTTGCCGAt                          >  5:205236/1‑136 (MQ=255)                                                                                                                  aaCCGCACCCTGGAGCGCGCCAGCATCCTGGCCGAGCAGTTCGGCGCGCATGCCGTGCTGCTGGCAGACATCCCCCAGGAACTGGCCAACAGTGACATCGTGATCAGTTCCACCGCCAGCCAGTTGCCGATTCTgg                     >  6:186499/1‑136 (MQ=255)                                                                                                                       cACCCTGGAGCGCGCCAGCATCCTGGCCGAGCAGTTCGGCGCGCATGCCGTGCTGCTGGCAGACATCCCCCAGGAACTGGCCAACAGTGACATCGTGATCAGTTCCACCGCCAGCCAGTTGCCGATTCTGGGCAAg                >  6:203817/1‑136 (MQ=255)                                                                                                                        ccccTGGAGCGCGCCAGCATCCTGGCCGAGCAGTTCGGCGCGCATGCCGTGCTGCTGGCAGACATCCCCCAGGAACTGGCCAACAGTGACATCGTGATCAGTTCCACCGCCAGCCAGTTGCCGATTCTGGGCAAgg               <  4:305989/135‑1 (MQ=255)                                                                                                                        aCCCTGGAGCGCGCCAGCATCCTGGCCGAGCAGTTCGGCGCGCATGCCGTGCTGCTGGCAGACATCCCCCAGGAACTGGCCAACAGTGACATCGTGATCAGTTCCACCGCCAGCCAGTTGCCGATTCTGGGCAAgg               <  7:172917/136‑1 (MQ=255)                                                                                                                        aCCCTGGAGCGCGCCAGCATCCTGGCCGAGCAGTTCGGCGCGCATGCCGTGCTGCTGGCAGACATCCCCCAGGAACTGGCCAACAGTGACATCGTGATCAGTTCCACCGCCAGCCAGTTGCCGATTCTGGGCAAgg               <  3:292548/136‑1 (MQ=255)                                                                                                                        aCCCTGGAGCGCGCCAGCATCCTGGCCGAGCAGTTCGGCGCGCATGCCGTGCTGCTGGCAGACATCCCCCAGGAACTGGCCAACAGTGACATCGTGATCAGTTCCACCGCCAGCCAGTTGCCGATTCTGGGCAAgg               <  8:245916/136‑1 (MQ=255)                                                                                                                        aCCCTGGAGCGCGCCAGCATCCTGGCCGAGCAGTTCGGCGCGCATGCCGTGCTGCTGGCAGACATCCCCCAGGAACTGGCCAACAGTGACATCGTGATCAGTTCCACCGCCAGCCAGTTGCCGATTCTGGGCAAgg               <  1:59511/136‑1 (MQ=255)                                                                                                                          ccTGGAGCGCGCCAGCATCCTGGCCGAGCAGTTCGGCGCGCATGCCGTGCTGCTGGCAGACATCCCCCAGGAACTGGCCAACAGTGACATCGTGATCAGTTCCACCGCCAGCCAGTTGCCGATTCTGGGCAAGGgc             <  7:34293/136‑1 (MQ=255)                                                                                                                              gAGCGCGCCAGCATCCTGGCCGAGCAGTTCGGCGCGCATGCCGTGCTTCTGGCAGACATCCCCCAGGAACTGGCCAACAGTGACATCGTGATCAGTTCCACCGCCAGCCAGTTGCCGATTCTGGGCAAGGgcgc           <  5:33612/134‑1 (MQ=255)                                                                                                                              gAGCGCGCCAGCATCCTGGCCGAGCAGTTCGGCGCGCATGCCGTGCTGCTGGCAGACATCCCCCAGGAACTGGCCAACAGTGACATCGTGATCAGTTCCACCGCCAGCCAGTTGCCGATTCTGGGCAAGGgcgc           <  3:318345/134‑1 (MQ=255)                                                                                                                                    gcCAGCATCCTGGCCGAGCAGTTCGGCGCGCATGCCGTGCTGCTGGCAGACATCCCCCAGGAACTGGCCAACAGTGACATCGTGATCAGTTCCACCGCCAGCCAGTTGCCGATTCTGGGCAAGGGCGCGGTCgaga   <  1:257369/136‑1 (MQ=255)                                                                                                                                    gcCAGCATCCTGGCCGAGCAGTTCGGCGCGCATGCCGTGCTGCTGGCAGACATCCCCCAGGAACTGGCCAACAGTGACATCGTGATCAGTTCCACCGCCAGCCAGTTGCCGATTCTGGGCAAGGGCGCGGTCga     >  7:211765/1‑134 (MQ=255)                                                                                                                                    gcCAGCATCCTGGCCGAGCAGTTCGGCGCGCATGCCGAGCTGCTGGCAGACATCCCCCAGGAACTGGCCAACAGTGACATCGTGATCAGTTCCACCGCCAGCCAGTTGCCGATTCTGGGCAAGGGCGCGGTCgaga   <  5:92159/136‑1 (MQ=255)                                                                                                                                     ccAGCATCCTGGCCGAGCAGTTCGGCGCGCATGCCGTGCTGCTGGCAGACATCCCCCAGGAACTg                                                                         <  2:155842/65‑1 (MQ=255)                                                                                                                                     ccAGCATCCTGGCCGAGCAGTTCGGCGCGCATGCCGTGCTGCTGGCAGACATCCCCCAGGAACTg                                                                         >  1:155842/1‑65 (MQ=255)                                                                                                                                     ccAGCATCCTGGCCGAGCAGTTCGGCGCGCATGCCGTGCTGCTGGCAGACATCCCCCAGGAACTGGCCAACAGTGACATCGTGATCAGTTCCACCGCCAGCCAGTTGCCGATTCTGGGCAAGGGCGCGGTCgagcg  >  6:165752/1‑134 (MQ=255)                                                                                                                                     ccAGCATCCTGGCCGAGCAGTTCGGCGCGCATGCCGTGCTGCTGGCAGACATCCCCCAGGAACTGGCCAACAGTGACATCGTGATCAGTTCCACCGCCAGCCAGTTGCCGATTCTGGGCAAGGGCGCGGTCGagag  >  5:204515/1‑136 (MQ=255)                                                                                                                                      |                                                                                                                                       AGATTTTCGCCGACCTGGGCCGCAGCCAGGCCCTGCTGATCGGTGCCGGCGAAACCATCACCCTGGTCGCCCGCCACCTGCACGAGCAGGGCGTACGCCGTATCGTGGTGGCCAACCGCACCCTGGAGCGCGCCAGCATCCTGGCCGAGCAGTTCGGCGCGCATGCCGTGCTGCTGGCAGACATCCCCCAGGAACTGGCCAACAGTGACATCGTGATCAGTTCCACCGCCAGCCAGTTGCCGATTCTGGGCAAGGGCGCGGTCGAGAG  >  ACB143/844789‑845056

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑