Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 634,781 Δ1 bp 7.8% coding (121/1089 nt) ydjJ ← putative zinc‑type alcohol dehydrogenase‑likeprotein YdjJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT2440634,7810C. 7.8% 177.2 / 8.2 51coding (121/1089 nt)ydjJputative zinc‑type alcohol dehydrogenase‑likeprotein YdjJ
Reads supporting (aligned to +/- strand):  ref base C (19/28);  new base . (2/2);  total (21/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01

GATGTGGCCGCAGAATTCGTGGCCGAGGATGCACTGGCCCTGAATGCCGGTCAGCGGGTGCGGGGCCTCTACCGGGATGAACACCGGGCCGGCAACATATTCGTGCAGGTCGGAGCCGCAGATGCCGCACCAGTCCACCTTGATCTGCACCCAGCCCGGCGCAGGGTCGGCCGGCAAAGGTACCTGTTCGACACGAATATCGTGGCGGCCATGCCAGACGGCCGCGCGCATGTGGGTGTGGCTCAGGTCATTCATTGTTGTTCTCC  >  KT2440/634647‑634912
                                                                                                                                      |                                                                                                                                   
gATGTGGCCGCAGAATTCGTGGCCGAGGATGCACTGGCCCTGAATGCCGGTCAGCGGGTGCGGGGCCTCTACCGGGATGAACACCGGGCCGGCAACATATTCGTGCAGGTCGGAGCCGCAGATGCCGCACCAGTc                                                                                                                                     >  7:245635/1‑135 (MQ=255)
 aTGTGGCCGCAGAATTCGTGGCCGAGGATGCACTGGCCCTGAATGCCGGTCAGCGGGTGCGGGGCCTCTACCGGGATGAACACCGGGCCGGCAACATATTCGTGCAGGTCGGAGCCGCAGATGCCGCACCAGTcc                                                                                                                                    <  5:325426/135‑1 (MQ=255)
 aTGTGGCCGCAGAATTCGTGGCCGAGGATGCACTGGCCCTGAATGCCGGTCAGCGGGTGCGGGGCCTCTACCGGGATGAACACCGGGCCGGCAACATATTCGTGCAGGTCGGAGCCGCAGATGCCGCACCAGTcc                                                                                                                                    >  7:125875/1‑135 (MQ=255)
      gCCGCAGAATTCGTGGCCGAGGATGCACTGGCCCTGAATGCCGGTCAGCGGGTGCGGGGCCTCTACCGGGATGAACACCGGGCCGGCAACATATTCGTGCAGGTCGGAGCCGCAGATGCCGCACCAGTCCACCtt                                                                                                                               <  1:295849/135‑1 (MQ=255)
       ccGCAGAATTCGTGGCCGAGGATGCACTGGCCCTGAATGCCGGTCAGCGGGTGCGGGGCCTCTACCGGGATGAACACCGGGCCGGCAACATATTCGTGCAGGTCGGAGCCGCAGATGCCGCACCAGTCCACCTTg                                                                                                                              >  7:163814/1‑135 (MQ=255)
              aTTCGTGGCCGAGGATGCACTGGCCCTGAATGCCGGTCAGCGGGTGCGGGGCCTCTACCGGGATGAACACCGGGCCGGCAACATATTCGTGCAGGTCGGAGCCGCAGATGCCGCACCAGTCCTCCTTGATCTGCa                                                                                                                       <  8:189172/135‑1 (MQ=255)
                 cGTGGCCGAGGATGCACTGGCCCTGAATGCCGGTCAGCGGGTGCGGGGCCTCTACCGGGATGAACACCGGGCCGGCAACATATTCGTGCAGGTCGGAGCCGCAGATGCCGCACCAGTCCACCTTGATCTGCACCCa                                                                                                                   <  4:16638/136‑1 (MQ=255)
                  gTGGCCGAGGATGCACTGGCCCTGAATGCCGGGCAGCGGGTGCGGGGCCTCTACCGGGATGAACACCGGGCCGGCAACATATTCGTGCAGGTCGGAGCCGCAGATGCCGCACCAGTCCACCTTGATCTGCACCCa                                                                                                                   <  3:274631/135‑1 (MQ=255)
                            aTGCACTGGCCCTGAATGCCGGTCAGCGGGTGCGGGGCCTCTACCGGGATGAACACCGGGCCGGCAACATATTCGTGCAGGTCGGAGCCGCAGATGCCGCACCAGTCCACCTTGATCTGCACCCAGCCCGGCGCa                                                                                                         >  7:202686/1‑135 (MQ=255)
                                      ccTGAATGCCGGTCAGCGGGTGCGGGGCCTCTACCGGGATGAACACCGGGCCGGCAACATATTCGTGCAGGTCGGAGCCGCAGATGCCGCACCAGTCCACCTTGATCTGCACCCAGCCCGGCGCAGGGTcggccg                                                                                               >  6:63733/1‑135 (MQ=255)
                                       cTGAATGCCGGTCAGCGGGTGCGGGGCCTCTACCGGGATGAACACCGGGCCGGCGACATATTCGTGCAGGTCGGAGCCGCAGATGCCGCACCAGTCCACCTTGATCTGCACCCAGCCCGGCGCAGGGTcggccg                                                                                               >  1:125099/1‑134 (MQ=255)
                                       cTGAATGCCGGTCAGCGGGTGCGGGGCCTCTACCGGGATGAACACCGGGCCGGCAACATATTCGTGCAGGTCGGAGCCGCAGATGCCGCACCAGTCCACCTTGATCTGCACCCAGCCCGGCGCAGGGTcggccg                                                                                               >  8:273647/1‑134 (MQ=255)
                                       cTGAATGCCGGTCAGCGGGTGCGGGGCCTCTACCGGGATGAACACCGGGCCGGCAACATATTCGTGCAGGTCGGAGCCGCAGATGCCGCACCAGTCACCTTGATCTGCACCCAGCCCg                                                                                                              >  3:76785/1‑118 (MQ=255)
                                       cTGAATGCCGGTCAGCGGGTGCGGGGCCTCTACCGGGATGAACACCGGGCCGGCAACATATTCGTGCAGGTCGGAGCCGCAGATGCCGCACCAGTCACCTTGATCTGCACCCAGCCCg                                                                                                              >  3:81854/1‑118 (MQ=255)
                                       cTGAATGCCGGTCAGCGGGTGCGGGGCCTCTACCGGGATGAACACCGGGCCGGCAACATATTCGTGCAGGTCGGAGCCGCAGATGCCGCACCAGTCACCTTGATCTGCACCCAGCCCg                                                                                                              <  4:81854/118‑1 (MQ=255)
                                       cTGAATGCCGGTCAGCGGGTGCGGGGCCTCTACCGGGATGAACACCGGGCCGGCAACATATTCGTGCAGGTCGGAGCCGCAGATGCCGCACCAGTCACCTTGATCTGCACCCAGCCCg                                                                                                              <  4:76785/118‑1 (MQ=255)
                                             gCCGGTCAGCGGGTGCGGGGCCTCTACCGGGATGAACACCGGGCCGGCAACATATTCGTGCAGGTCGGAGCCGCAGATGCCGCACCAGTCCACCTTGATCTGCACCCAGCCCGGCGCAGGGTCGGCCGGCAAAg                                                                                         >  5:352423/1‑134 (MQ=255)
                                                 gTCAGCGGGTGCGGGGCCTCTACCGGGATGAACACCGGGCCGGCAACATATTCGTGCAGGTCGGAGCCGCAGATGCCGCACCAGTCCACCTTGATCTGCACCCAGCCCGGCGCAGGGTCGGCCGGCAAAGGTAcc                                                                                    >  6:45466/1‑135 (MQ=255)
                                                  tCAGCGGGTGCGGGGCCTCTACCGGGATGAACACCGGGCCGGCAACATATTCGTGCAGGTCGGAGCCGCAGATGCCGCACCAGTCCACCTTGATCTGCACCCAGCCCGGCGCAGGGTCGGCCGGCAAAGGTACCt                                                                                   >  8:49312/1‑135 (MQ=255)
                                                   cAGCGGGTGCGGGGCCTCTACCGGGATGAACACCGGGCCGGCAACATATTCGTGCAGGTCGGAGCCGCAGATGCCGCACCAGTCCACCTTGATCTGCACCCAGCCCGGCGCAGGGTCGGCCGGCAAAGGTACCTg                                                                                  <  8:40778/135‑1 (MQ=255)
                                                   cAGCGGGTGCGGGGCCTCTACCGGGATGAACACCGGGCCGGCAACATATTCGTGCAGGTCGGAGCCGCAGATGCCGCACCAGTCCACCTTGATCTGCACCCAGCCCGGCGCAGGGTCGGCCGGCAAAGGTACCTg                                                                                  <  5:101672/135‑1 (MQ=255)
                                                     gCGGGTGCGGGGCCTCTACCGGGATGAACACCGGGCCGGCAACATATTCGTGCAGGTCGGAGCCGCAGATGCCGCACCAGTCCACCTTGATCTGCACCCAGCCCGGCGCAGGGTCGGCCGGCAAAGGTACCTGtt                                                                                <  5:182913/135‑1 (MQ=255)
                                                     gCGGGTGCGGGGCCTCTACCGGGATGAACACCGGGCCGGCAACATATTCGTGCAGGTCGGAGCCGCAGATGCCGCACCAGTCCACCTTGATCTGCACCCAGCCCGGCGCAGGGTCGGCCGGCAAAGGTACCTGtt                                                                                <  7:273647/135‑1 (MQ=255)
                                                     gCGGGTGCGGGGCCTCTACCGGGATGAACACCGGGCCGGCAACATATTCATGCAGGTCGGAGCCGCAGATGCCGCACCAGTCCACCTTGATCTGCACCCAGCCCGGCGCAGGGTCGGCCGGCAAAGGTACCTGtt                                                                                <  5:67054/135‑1 (MQ=255)
                                                             ggggCCTCTACCGGGATGAACACCGGGCCGGCAACATATTCGTGCAGGTCGGAGCCGCAGATGCCGCACCAGTCCACCTTGATCTGCACCCAGCCCGGCGCAGGGTCGGCCGGCAAAGGTACCTGTTCGACACGa                                                                        <  1:322256/135‑1 (MQ=255)
                                                                  ctctACCGGGATGAGCACCGGGCCGGCAACATATTCGTGCAGGTCGGAGCCGCAGATGCCGCACCAGTCCACCTTGATCTGCACCCAGCCCGGCGCAGGGTCGGCCGGCAAAGGTACCTGTTCGACACGAATATc                                                                   <  5:230056/135‑1 (MQ=255)
                                                                       ccGGGATGAACACCGGGCCGGCAACATATTCGTGCAGGTCGGAGCCGCAGATGCCGCACCAGTCCACCTTGATCTGCACCCAGCCCGGCGCAGGGTCGGCCGGCAAAGGTACCTGTTCGACACGAATATCGTggc                                                              >  8:158417/1‑135 (MQ=255)
                                                                        cGGGATGAACACCGGGCCGGCAACATATTCGTGCAGGTCGGAGCCGCAGATGCCGCACCAGTCCACCTTGATCTGCACCCAGCCCGGCGCAGGGTCGGCCGGCAAAGGTACCTGTTCGACACGAATATCGTggcg                                                             >  6:180264/1‑135 (MQ=255)
                                                                         gggATGAACACCGGGCCGGCAACATATTCGTGCAGGTCGGAGCCGCAGATGCCGCACCAGTCCACCTTGATCTGCACCCAGCCCGGCGCAGGGTCGGCCGGCAAAGGTACCTGTTCGACACGAATATCGTggcgg                                                            <  2:125099/135‑1 (MQ=255)
                                                                                       gCCGGCAACATATTCGTGCAGGTCGGAGCCGCAGATGCCGCACCAGTCCACCTTGATCTGCACCCAGCCCGGCGCAGGGTCGACCGGCAAAGGTACCTGTTCGACACGAATATCGTGGCGGCCATGCCAGACGGc                                              <  5:345833/135‑1 (MQ=255)
                                                                                         cGGCAACATATTCGTGCAGGGCGGAGCCGCAGATGCCGCACCAGTCCACCTTGATCTGCACCCAGCCCGGCGCAGGGTCGGCCGGCAAAGGTACCTGTTCGACACGAATATCGTGGCGGCCATGCCAGACGGCcg                                            <  5:127560/135‑1 (MQ=255)
                                                                                          ggCAACATATTCGTGCAGGGCGGAGCCGCAGATGCCGCACCAGTCCACCTTGATCTGCACCCAGCCCGGCGCAGGGTCGGCCGGCAAAGGTACCTGTTCGACACGAATATCGTGGCGGCCATGCCAGACGGCcgc                                           <  2:303378/135‑1 (MQ=255)
                                                                                            cAACATATTCGTGCAGGGCGGAGCCGCAGATGCCGCACCAGTCCACCTTGATCTGCACCCAGCCCGGCGCAGGGTCGGCCGGCAAAGGTACCTGTTCGACACGAATATCGTGGCGGCCATGCCAGACGGCcgcgcg                                        <  4:292475/136‑1 (MQ=255)
                                                                                               cATATTCGTGCAGGTCGGAGCCGCAGATGCCGCACCAGTCCACCTTGATCTCCACCCAGCCCGGCGCAGGGTCGGCCGGCAAAGGTACCTGTTCGACACGAATATCGTGGCGGCCATGCCAGACGGCCGCGCGCa                                      <  5:303991/135‑1 (MQ=255)
                                                                                                   ttCGTGCAGGTCGGAGCCGCAGATGCCGCACCAGTCCACCTTGATCTGCACCCAGCCCGGCGCAGGGTCGGCCGGCAAAGGTACCTGTTCGACACGAATATCGTGGCGGCCATGCCAGACGGCCGCGCGCAtgtg                                  <  4:48574/135‑1 (MQ=255)
                                                                                                         cAGGGCGGAGCCGCAGATGCCGCACCAGTCCACCTTGATCTGCACCCAGCCCGGCGCAGGGTCGGCCGGCAAAGGTACCTGTTCGACACGAATATCGTGGCGGCCATGCCAGACGGCCGCGCGCATGTGGGtgtg                            <  1:43643/135‑1 (MQ=255)
                                                                                                              cGGAGCCGCAGATGCCGCACCAGTCCACCTTGATCTGCACCCAGCCCGGCGCAGGGTCGGCCGGCAAAGGTACCTGTTCGACACGAATATCGTGGCGGCCATGCCAGACGGCCGCGCGCATGTGGGTGTGGCTCAg                      <  3:199500/136‑1 (MQ=255)
                                                                                                                 aGCCGCAGATGCCGCACCATGCCACCTTGATCTGCCCCCAGCCCGGCGCAGGGTCGGCCGGCAAAGGTACCTGTTCGACACGAATATCGTGGCGGCCAGGCCAGACGGCCGCGCGCATGTGGGTGTGGCTCAgggc                   <  4:291374/136‑3 (MQ=255)
                                                                                                                 aGCCGCAGATGCCGCACCAGTCCACCTTGATCTGCACCCAGCCCGGCGCAGGGTCGGCCGGCAAAGGTACCTGTTCGACACGAATATCGTGGCGGCCATGCCAGACGGCCGCGCGCATGTGGGTGTGGCTCAgg                     >  5:133108/1‑134 (MQ=255)
                                                                                                                 aGCCGCAGATGCCGCACCAGTCCACCTTGATCTGCACCCAGCCCGGCGCAGGGTCGGCCGGCAAAGCTACCTGTTCGACACGAATATCGTGGCGGCCATGCCAGACGGCCGCGCGCATGTGGGTGTGGCTCAgg                     >  2:258425/1‑134 (MQ=255)
                                                                                                                  gCCGCAGATGCCGCACCAGTCCACCTTGATCTGCACCCAGCCCGGCGCAGGGTCGGCCGGCAAAGGTACCTGTTCGACACGAATATCGTGGCGGCCATGCCAGACGGCCGCGCGCATGTGGGTGTGGCTCAGGtc                   <  6:289703/135‑1 (MQ=255)
                                                                                                                  gCCGCAGATGCCGCACCAGTCCACCTTGATCTGCACCCAGCCCGGCGCAGGGTCGGCCGGCAAAGGTACCTGTTCGACACGAATATCGTGGCGGCCATGCCAGACGGCCGCGCGCATGTGGGTGTGGCTCAGGtc                   <  5:317408/135‑1 (MQ=255)
                                                                                                                     gCAGATGCCGCACCAGTCCACCTTGATCTGCACCCAGCCCGGCGCAGGGTCGGCCGGCAAAGGTACCTGTTCGACACGAATATCGTGGCGGCCATGCCAGACGGCCGCGCGCATGTGGGTGTGGCTCAGGtcatt                <  7:158417/135‑1 (MQ=255)
                                                                                                                         aTGCCGCACCAGTCCCCCTTGATCTGCACCCAGCCCGGCGCAGGGTCGGCCGGCAAAGGTACCTGTTCGACACGAATATCGTGGCGGCCATGCCAGACGGCCGCGCGCATGTGGGTGTGGCTCAGGTcattcatt            >  6:21485/1‑135 (MQ=255)
                                                                                                                         aTGCCGCACCAGTCCACCTTGATCTGCACCCAGCCCGGCGCAGGTTCGGCCGGCAAAGGTACCTGTTCGACACGAATATCGTGGCGGCCATGCCAGACGGCCGCGCGCATGTGGGTGTGGCTCAGGTCATTCAttg           <  4:306244/136‑1 (MQ=255)
                                                                                                                         aTGCCGCACCAGTCCACCTTGATCTGCACCCAGCCCGGCGCAGGGTCGGCCGGCAAAgg                                                                                        >  1:298594/1‑59 (MQ=255)
                                                                                                                         aTGCCGCACCAGTCCACCTTGATCTGCACCCAGCCCGGCGCAGGGTCGGCCGGCAAAgg                                                                                        <  2:298594/59‑1 (MQ=255)
                                                                                                                         aTGCCGCACCAGTCCACCTTGATCTGCACCCAGCCCGGCGCAGGGTCGGCCGGCAAAGGTACCTGTTCGACACGAATATCGTGGCGGCCATGCCAGACGGCCGCGCGCATGTGGGTGTGGCTCAGGTcattcatt            >  5:168198/1‑135 (MQ=255)
                                                                                                                         aTGCCGCACCAGTCCACCTTGATCTGCACCCAGCCCGGCGCAGGGTCGGCCGGCAAAGGTACCTGTTCGACACGAATATCGTGGCGGCCATGCCAGACGGCCGCGCGCATGTGGGTGTGGCTCAGGTCATTCAttg           >  3:239804/1‑136 (MQ=255)
                                                                                                                         aTGCCGCACCAGTCCACCTTGATCTGCACCCAGCCCGGCGCAGGGTCGGCCGGCAAAGGTACCTGTTCGACACGAATATCGTGGCGGCCATGCCAGACGGCCGCGCGCATGTGGGTGTGGCTCAGGTCATTCAttg           <  4:36111/136‑1 (MQ=255)
                                                                                                                         aTGCCGCACCAGTCCACCTTGATCTGCACCCAGCCCGGCGCAGGGTCGGCCGGCAAAGGTACCTGTTCGACACGAATATCGTGGCGGCCATGCCAGACGGCCGCGCGCATGTGGGTGTGGCTCAGGTCATTCAttg           >  4:329367/1‑136 (MQ=255)
                                                                                                                         aTGCCGCACCAGTCCACCTTGATCTGCACCCAGCCCGGCGCAGGGTCGGCCGGCAAAGGTACCTGTTCGACACGAATATCGTGGCGGCCATGCCAGACGGCCGCGCGCATGTGGGTGTGGCTAATGTcattcatt            >  5:161857/1‑135 (MQ=255)
                                                                                                                                  cAGTCCACCTTGATCTGCACCCAGCCCGGCGCAGGGTCGGCCGGCAAAGGTACCTGTTCGACACGAATATCGTGGCGGCCATGCCAGACGGCCGCGCGCATGTGGGTGTGGCTCAGGTCATTCATTGTTGTtct    >  6:154340/1‑134 (MQ=255)
                                                                                                                                   aGTCTACCTTGATCTGCACCCAGCCCGGCGCAGGGTCGGCCGGCAAAGGTACCTGTTCGACACGAATATCGTGGCGGCCATGCCAGACGGCCGCGCGCATGTGGGTGTGGCTCAGGTCATTCATTGTTGTTCTcc  <  1:88656/135‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                   
GATGTGGCCGCAGAATTCGTGGCCGAGGATGCACTGGCCCTGAATGCCGGTCAGCGGGTGCGGGGCCTCTACCGGGATGAACACCGGGCCGGCAACATATTCGTGCAGGTCGGAGCCGCAGATGCCGCACCAGTCCACCTTGATCTGCACCCAGCCCGGCGCAGGGTCGGCCGGCAAAGGTACCTGTTCGACACGAATATCGTGGCGGCCATGCCAGACGGCCGCGCGCATGTGGGTGTGGCTCAGGTCATTCATTGTTGTTCTCC  >  KT2440/634647‑634912

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: