Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 699,520 Δ1 bp 6.2% coding (1947/2301 nt) yebT → Intermembrane transport protein YebT

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT2440699,5200T. 6.2% 194.0 / 6.6 64coding (1947/2301 nt)yebTIntermembrane transport protein YebT
Reads supporting (aligned to +/- strand):  ref base T (30/29);  new base . (2/2);  total (32/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTGCAGCCAGCAGCCAAGAACCGTGGGCCACAGCGCGATTTCATCGCCCTGGCCGAAGCACCGGAGGTAGCAGGGCCAGAGGTCGGCCTGCCATTGACGCTCAGTGCGCCACGCCGTGGCTCGATCAAGCCGGGTGTGCCGGTTACCTATCGTGAGGTTACGGTGGGCAAGGTGACAGGCTTCGAGCTGGGCCAGAGCGCTGACCGCGTGCTGATTCATATCCTCATCGAGCCGCGCTATGCCGCCTTGGTGCGCAGCGGTAGCCGCTTC  >  KT2440/699386‑699655
                                                                                                                                      |                                                                                                                                       
gTGCAGCCAGCAGCCAAGAACCGTGGGCCACAGCGCGATTTCATCGCCCTGGCCGAAGCACCGGAGGTAGCAGGGCCAGAGGGCGGCCTGCCATTGACGCTCAGTGCGCCACGCCGTGGCTCGATCAAGCCGGgtg                                                                                                                                        <  3:158914/136‑1 (MQ=255)
    agccagcagccaAGAACCGTGGGCCACAGCGCGATTTCATCGCCCTGGCCGAAGCACCGGAGGTAGCAGGGCCAGAGGTCGGCCTGCCATTGACGCTCAGTGCGCCACGCCGTGGCTCGATCAAGCCGGGtgtg                                                                                                                                      <  2:4743/134‑1 (MQ=255)
    agccagcagccaAGAACCGTGGGCCACAGCGCGATTTCATCGCCCTGGCCGAAGCACCGGAGGTAGCAGGGCCAGAGGTCGGCCTGCCATTGACGCTCAGTGCGCCACGCCGTGGCTCGATCAAGCCGGGTGTGcc                                                                                                                                    >  4:95143/1‑136 (MQ=255)
    agccagcagccaAGAACCGTGGGCCACAGCGCGATTTCATCGCCCTGGCCGAAGCACCGGAGGTAGCAGGGCCAGAGGTCGGCCTGCCATTGACGCTCAGTGCGCCACGCCGTGGCTCGATCAAGCCGGGTGTGc                                                                                                                                     >  8:90911/1‑135 (MQ=255)
    agccagcagccaAGAACCGTGGGCCACAGCGCGATTTCATCGCCCTGGCCGAAGCACCGGAGGTAGCAGGGCCAGAGGTCGGCCTGCCATTGACGCTCAGTGCGCCACGCCGTGGCTCGATCAAGCCGGGTGTGc                                                                                                                                     >  2:137220/1‑135 (MQ=255)
    agccagcagccaAGAACCGTGGGCCACAGCGCGATTTCATCGCCCTGGCCGAAGCACCGGAGGTAGCAGGGCCAGAGGTCGGCCTGCCATTGACGCTCAGTGAGCCACGCCGTGGCTCGATCAAGCCGGGTGTGcc                                                                                                                                    >  4:105459/1‑136 (MQ=255)
      ccagcagccaAGAACCGTGGGCCACAGCGCGATTTCATCGCCCTGGCCGAAGCACCGGAGGTAGCAGGGCCAGAGGTCGGCCTGCCATTGACGCTCAGTGCGCCACGCCGTGGCTCGATCAAGCCGGGTGTGCCg                                                                                                                                   >  6:358767/1‑135 (MQ=255)
        agcagacaAGAACAGTGGGCCAGAGCGCGATTTCATCGCCCTGGCCGAAGCACCGGATGTAGCAGGGCCAGAGGTCGGCCTGCCATTGACGCTCAGTGCGCCACGCCGTGGCTCGATCAAGCCGGGTGTGCCGGt                                                                                                                                 <  2:101192/135‑1 (MQ=255)
             ccaAGAACCGTGGGCCACAGCGCGATTTCATCGCCCTGGCCGAAGCACCGGAGGTAGCAGGGCCAGAGGTCGGCCTGCCATTGACGCTCAGTGCGCCACGCCGTGGCGCGATCAAGCCGGGTGTGCCGGTTACCTa                                                                                                                           <  3:277000/136‑1 (MQ=255)
              caAGAACCGTGGGCCACAGCGCGATTTCATCGCCCTGGCCGAAGCACCGGAGGTAGCAGGGCCAGAGGTCGGCCTGCCATTGACGCTCAGTGCGCCACGCCGTGGCTCGATCAAGCCGGGTGTGCCGGTTACCTa                                                                                                                           <  7:94404/135‑1 (MQ=255)
              caAGAACCGTGGGCCACAGCGCGATTTCATCGCCCTGGCCGAAGCACCGGAGGTAGCAGGGCCAGAGGTCGGCCTGCCATTGACGCGCAGTGCGCCACGCCGTGGCTCGATCAAGCCGGGTGTGCCGGTTACCTa                                                                                                                           <  6:207734/135‑1 (MQ=255)
               aaGAACCGTGGGCCACAGCGCGATTTCATCGCCCTGGCCGAAGCACCGGAGGTAGCAGGGCCAGAGGTCGGCCTGCCATTGACGCTCAGTGCGCCACGCCGTGGCTCGATCAAGCCGGGTGTGCCGGTTACCTAt                                                                                                                          <  7:71157/135‑1 (MQ=255)
                          gCCACAGCGCGATTTCATCGCCCTGGCCGAAGCACCGGAGGTAGCAGGGCCAGAGGTCGGCCTGCCATTGACGCTCAGTGCGCCACGCCGTGGCTCGATCAAGCCGGGTGTGCCGGTTACCTATCGTGAGGTTAc                                                                                                               >  7:283760/1‑135 (MQ=255)
                          gCCACAGCGCGATTTCATCGCCCTGGCCGAAGCACCGGAGGTAGCAGGGCCAGAGGTCGGCCTGCCATTGACGCTCAGTGCGCCACGCCGTGGCTCGATCAAGCCGGGTGTGCCGGTTACCTATCGTGAGGTTAc                                                                                                               >  5:358615/1‑135 (MQ=255)
                          gCCACAGCGCGATTTCATCGCCCTGGCCGAAGCACCGGAGGTAGCAGGGCCAGAGGTCGGCCTGCCATTGACGCTCAGTGCGCCACGCCGTGGCTCGATCAAGCCGGGTGTGCCGGTTACCTATCGTGAGGTTACg                                                                                                              >  3:335241/1‑136 (MQ=255)
                          gCCACAGCGCGATTTCATCGCCCTGGCCGAAGCACCGGAGGTAGCAGGGCCAGAGGTCGGCCTGCCATTGACGCTCAGTGCGCCACGCCGTGGCTCGATCAAGCCGGGTGTGCCGGTTACCTATCGTGAGGTTACg                                                                                                              >  4:183834/1‑136 (MQ=255)
                          gCCACAGCGCGATTTCATCGCCCTGGCCGAAGCACCGGAGGTAGCAGGGCCAGAGGTCGGCCTGCCATTGACGCTCAGTGCGCCACGCCGTGGCTCGATCAAGCCGGGTGTGCCGGTTACCTATCGTGAGGTTACg                                                                                                              >  3:252925/1‑136 (MQ=255)
                              caGCGCGATTTCATCGCCCTGGCCGAAGCACCGGAGGTAGCAGGGCCAGAGGTCGGCCTGCCATTGACGCTCAGTGCGCCACGCCGTGGCTCGATCAAGCCGGGTGTGCCGGTTACCTATCGTGAGGTTACGGTg                                                                                                           <  6:208559/135‑1 (MQ=255)
                               aGCGCGATTTCATCGCCCTGGCCGAAGCACCGGAGGTAGCAGGGCCAGAGGTCGGCCTGCCATTGACGCTCAGTGCGCCACGCCGTGGCTCGATCAAGCCGGGTGTGCCGGTTACCTATCGTGAGGTTACGGTgg                                                                                                          >  2:291371/1‑135 (MQ=255)
                                  gcgATTTCATCGCCCTGGCCGAAGCACCGGAGGTAGCAGGGCCAGAGGGCGGCCTGCCATTGCCGCTCAGTGCGCCACGCCGTGGCTCGCTCACGCCGG‑‑GTGCCGGTTACCTAGCGTGAGGTTACGGTGGGCAAgg                                                                                                    >  4:46221/1‑136 (MQ=255)
                                  gcgATTTAATCGCCCTGGCCGAATCACCGGAGGTAGCAGGGCCAGAGGTCGGCCTGCCATTGACGCTCAGTGCGCCACGCCGTGGCTAGATCAAGCCGG‑‑GTGCCGGTTACCTATCGTGAGGTTACGGTGGGCAAgg                                                                                                    >  4:51069/1‑136 (MQ=255)
                                      tttCATCGCCCTGGCCGAAGCACCGGAGGTAGCAGGGCCAGAGGTCGGCCTGCCATTGACGCTCAGTGCGCCACGCCGTGGCTCGATCAAGCCGGGTGTGCCGGTTACCTATCGTGAGGTTACGGTGGGCAAGGt                                                                                                   <  8:283760/135‑1 (MQ=255)
                                      tttCATCGCCCTGGCCGAAGCACCGGAGGTAGCAGGGCCAGAGGTCGGCCTGCCATTGACGCTCAGTGCGCCACGCCGTGGCTCGATCAAGCCGGGTGTGCCGGTTACCTATCGTGAGGTTACGGTGGGCAAGGt                                                                                                   <  1:104666/135‑1 (MQ=255)
                                           tCGCCCTGGCCGAAGCACCGGAGGTAGCAGGGCCAGAGGTCGGCCTGCCATTGACGCTCAGTGCGCCACGCCGTGGCTCGATCAAGCCGGGTGTGCCGGTTACCTATCGTGAGGTTACGGTGGGCAAGGTGACAgg                                                                                             <  3:364129/136‑1 (MQ=255)
                                            cGCCCTGGCCGAAGCACCGGAGGTAGCAGGGCCAGAGGTCGGCCTGCCATTGACGCTCAGTGCGCCACGCCGTGGCTCGATCAAGCCGGGTGTGCCGGTTACCTATCGTGAGGTTACGGTGGGCAAGGTGACAgg                                                                                             <  1:142940/135‑1 (MQ=255)
                                              cccTGGCCGAAGCACCGGAGGTAGCAGGGCCAGAGGTCGGCCTGCCATTGACGCTCAGTGCGCCACGCCGTGGCTCAATCAAGCCGGGTGTGCCGGTTACCTATCGTGAGGTTACGGTGGGCAAGGTGACAGGCt                                                                                           >  5:100668/1‑135 (MQ=255)
                                                     cGAAGCACCGGAGGTAGCAGGGCCAGAGGTCGGCCTGCCATTGACGCTCAGTGCGCCACGCCGTGGCTCGATCAAGCCGGGTGTGCCGGTTACCTATCGTGAGGTTACGGTGGGCAAGGTGACAGGCTTCGAGCt                                                                                    >  2:296923/1‑135 (MQ=255)
                                                       aaGCACCGGAGGTAGCAGGGCCAGAGGTCGGCCTGCCATTGACGCTCAGTGCGCCACGCCGTGGCTCGATCAAGCCGGGTGTGCCGGTTACCTATCGTGAGGTTACGGTGGGCAAGGTGACAGGCTTCGAGCTgg                                                                                  <  8:189107/135‑1 (MQ=255)
                                                           aCCGGAGGTAGCAGGGCCAGAGGTCGGCCTGCCATTGACGCTCAGTGCGCCACGCCGTGGCTCGATCAAGCCGGGTGTGCCGGTTACCTATCGTGAGGTTACGGTGGGCAAGGTGACAGGCTTCGAGCTggg                                                                                 >  7:123827/1‑132 (MQ=255)
                                                           aCCGGAGGTAGCAGGGCCAGAGGTCGGCCTGCCATTGACGCTCAGTGCGCCACGCCGTGGCTCGATCAAGCCGGGTGTGCCGGTTACCTATCGTGAGGTTACGGTGGGCAAGGTGACAGGCTTCGAGCTGGGCCa                                                                              >  7:178945/1‑135 (MQ=255)
                                                           aCCGGAGGTAGCAGGGCCAGAGGTCGGCCTGCCATTGACGCTCAGTGCGCCACGCCGTGGCTCGATCAAGCCGGGTGTGCCGGTTACCTATCGTGAGGTTACGGTGGGCAAGGTGACAGGCTTCGAGCTGGCCCa                                                                              >  5:167234/1‑135 (MQ=255)
                                                            ccGGAGGTAGCAGGGCCAGAGGTCGGCCTGCCATTGACGCTCAGTGCGCCACGCCGTGGCTCGATCAAGCCGGGTGTGCCGGTTACCTATCGTGAGGTTACGGTGGGCAAGGTGACAGGCTTCGAGCTGGGcc                                                                               >  5:108061/1‑133 (MQ=255)
                                                             cGGAGGTAGCAGGGCCAGAGGTCGGCCTGCCATTGACGCTCAGTGCGCCACGCCGTGGCTCGATCTCGCCGGGTGTGCCGGTTACCTATCGTGAGGTTAGGGTGGGCAAGTTTACAGGCTTCGAGCGGGGCCaga                                                                            <  1:49935/135‑1 (MQ=255)
                                                                aGGTAGCAGGGCCAGAGGTCGGCCTGCCATTGACGCTCAGTGCGCCACGCCGTGGCTCGATCAAGCCGGGTGTGCCGGTTACCTATCGTGAGGTTACGGTGGGCAAGGTGACAGGCTTCGAGCTGGGCCAGAgcg                                                                         >  2:14535/1‑135 (MQ=255)
                                                                  gTAGCAGGGCCAGAGGTCGGCCTTCCATTGACGCTCAGTGCGCCACGCCGTGGCTCGATCAAGCCGGGTGTGCCGGTTACATATCGTGAGGTTACGGTGGGCAAGGTGACAGGCTTCGAGCTGGGCCAGAGCGCTg                                                                      <  4:12163/136‑1 (MQ=255)
                                                                   tAGCAGGGCCAGAGGTCGGCCTGCCATTGACGCTCAGTGCGCCACGCCGTGGCTCGATCAAGCCGGGTGTGCCGGTTACCTATCGTGAGGTTACGGTGGGCAAGGTGACAGGCTTCGAGCTGGGCCAGAGCGCTg                                                                      <  2:244760/135‑1 (MQ=255)
                                                                          gCCAGAGGTCGGCCTGCCATTGACGCTCAGTGCGCCACGCCGTGGCTCGATCAAGCCGGGTGTGCCGGTTACCTATCGTGAGGTTACGGTGGGCAAGGTGACAGGCTTCGAGCTGGGCCAGAGCGCTGACCGCGt                                                               <  2:234972/135‑1 (MQ=255)
                                                                          gCCAGAGGTCGGCCTGCCATTGACGCTCAGTGCGCCACGCCGTGGCGCGATCAAGCCGGGTGTGCCGGTTACCTATCGTGAGGTTACGGTGGGCAAGGTGACAGGCTTCGAGCTGGGCCAGAGCGCTGACCGCGt                                                               <  2:226410/135‑1 (MQ=255)
                                                                            cAGAGGTCGGCGTGCCATTGACGCTCAGTGCGCCACGCCGTGGCTAGATCAAGCCGGGTGTGCCGGTTACCTATCGTGAGGt                                                                                                                  <  6:338349/82‑1 (MQ=255)
                                                                            cAGAGGTCGGCCTGCCATTGACGCTCAGTGCGCCACGCCGTGGCTCGATCAAGCCGGGTGTGCCGGTTACCTATCGTGAGGt                                                                                                                  >  5:338349/1‑82 (MQ=255)
                                                                            cAGAGGTCGGCCTGCCATTGACGCTCAGTGCGCCACGCCGTGGCTCGATCAAGCCGGGTGTGCCGGTTACCTATCGTGAGGTTACGGTGGGCAAGGTGACAGGCTTCGAGCTGGGCCAGAGCGCTGACCGCGTGc                                                             >  7:38310/1‑135 (MQ=255)
                                                                            cAGAGGTCGGCCTGCCATTGACGCTCAGTGCGCCACGCCGTGGCTCGATCAAGCCGGGTGTGCCGGTTACCTATCGTGAGGTTACGGTGGGCAAGGTGACAGGCTTCGAGCTGGGCCAGAGCGCTGACCGCGTGCt                                                            >  3:46756/1‑136 (MQ=255)
                                                                              gagGTCGGCCTGCCATTGACGCTCAGTGCGCCACGCCGTGGCTCGATCAAGCCGGGTGTGCCGGTTACCTATCGTGAGGTTACGGTGGGCAAGGTGACAGGCTTCGAGCTGGGCCAGAGCGCTGACCGCGTGCTg                                                           <  1:305298/135‑1 (MQ=255)
                                                                                   cGGCCTGCCATTGACGCTCAGTGCGCCACGCCGTGGCTCGATCAAGCCGGGTGTGCCGGTTACCTATCGTGAGGTTACGGTGGGCAAGGTGACAGGCTTCGAGCTGGGCCAGAGCGCTGACCGCGTGCTGATTCa                                                      <  1:296923/135‑1 (MQ=255)
                                                                                       cTGCCATTGACGCTCAGTGCGCCACGCCGTGGCTCGATCAAGCCGGGTGTGCCGGTTACCTATCGTGAGGTTACGGTGGGCAAGGTGACAGGCTTCGAGCTGGGCCAGAGCGCTGACCGCGTGCTGATTCATATc                                                  >  1:66013/1‑135 (MQ=255)
                                                                                       cTGCCATTGACGCTCAGTGCGCCACGCCGTGGCTCGATCAAGCCGGGTGTGCCGGTTACCTATCGTGAGGTTACGGTGGGCAAGGTGACAGGCTGCGAGCTGGGCCAGAGCGCTGACCGCGTGCTGATTCATATcc                                                 >  4:217806/1‑136 (MQ=255)
                                                                                        tGCCATTGACGCTCAGTGCGCCACGCCGTGGCTCGATCAAGCCGGGTGTGCCGGGTACCTATCGTGAGGTTACGGTGGGCTAGGTGACAGGCTTCGAGCTGGGCCAGAGCGCTGACCGCGTGCTGATTCATATcc                                                 <  6:178413/135‑1 (MQ=255)
                                                                                                ccGCTCAGGGCGCCACGCCGTGGCGCGAGCAAGCCGGGGGCCGGTTACCTATCGTGAGGGTACGGGGGGCAAGGTGACAGGCTTCGAGCTGGGCCAGAGCGCTGACCGCGTGCTGATTCATATCCTCATCGAGcc                                       <  3:46221/134‑1 (MQ=255)
                                                                                                aCGCTCAGTGCGCCACGCCGTGGCTCGATCAAGCCGG‑‑GTGCCGGTTACCTATCGTGAGGTTACGGTGGGCAAGGTGACAGGCTTCGAGCTGGGCCAGAGCGCTGACCGCGTGCTGATTCATATCCTCATCGAGcc                                       <  3:51069/135‑1 (MQ=255)
                                                                                                 cGCTCAGTGCGCCACGCCGTGGCTCGATCAAGCCGGGTGTGCCGGTTACCTATCGTGAGGTTACGGTGGGCAAGGTGACAGGCTTCGAGCTGGGCCAGAGCGCTGACCGCGTGCTGATTCATATCCTCATCGAGcc                                       <  4:252925/136‑1 (MQ=255)
                                                                                                       gTGCGCCACGCCGTGGCGCGATCACGCCGGGTGTGCCGGTTACCTATCGTGAGGTTACGGTGGGCAAGGTGACAGGCTTCGAGCTGGGCCAGAGCGCTGACCGCGTGCTGATTCATATCCTCATCGAGCCGCGCt                                  <  7:233765/135‑1 (MQ=255)
                                                                                                          cgcCACGCCGTGGCTCGATCAAGCCGGGTGTGCCGGTTACCTATCGTGAGGTTACGGTGGGCAAGGTGACAGGCTTc                                                                                         <  6:341286/77‑1 (MQ=255)
                                                                                                          cgcCACGCCGTGGCTCGATCAAGCCGGGTGTGCCGGTTACCTATCGTGAGGTTACGGTGGGCAAGGTGACAGGCTTc                                                                                         >  5:341286/1‑77 (MQ=255)
                                                                                                             cACGCCGTGGCTCAATCAAGCCGGGTGTGCCGGTTACCTATCGTGAGGTTACGGTGGGCAAGGTGACAGGCTTCGAGCTGGGCCAGAGCGCTGACCGCGTGCTGATTCATATCCTCATCGAGCCGCGCTATgccg                            <  6:100668/135‑1 (MQ=255)
                                                                                                                 ccGTGGCTCGATCAAGCCGGGTGTGCCGGTTACCTATCGTGAGGTTACGGTGGGCAAGGTGACAGGCTTCGAGCTGGGCCAGAGCGCTGACCGCGTGCTGATTCATATCCTCATCGAGCCGCGCTATGCCGCCtt                        >  7:149203/1‑135 (MQ=255)
                                                                                                                  cGTGGCTCGATCAAGCCGGGTGTGCCGGTTACCTATCGTGAGGTTACGGTGGGCAAGGTGACAGGCTTCGAGCTGGGCCAGAGCGCTGACCGCGTGCTGATTCATATCCTCATCGAGCCGCGCTATGCCGCCTTg                       <  6:318776/135‑1 (MQ=255)
                                                                                                                   gTGGCTCGATCAAGCCGGGTGTGCCGGTTACCTATCGTGAGGTTACGGTTGGCAAGGTGACAGGCTTCGAGCTGGTCCAGAGCGCTGTCCGCGTGCTGATTCATATCCTCCTCGAGCCGCGCTATGCCGCCTTGGt                     <  3:77760/136‑1 (MQ=255)
                                                                                                                   gTGGCTCGATCAAGCCGGGTGTGCCGGTTACCTATCGTGAGGTTACGGTGGGCAAGGTGACAGGCTTCGAGCTGGGCCAGAGCGCTGACCGCGTGCTGATTCATATCCTCATCGAGCCGCGCTATGCCGCCTTGGt                     <  4:253299/136‑1 (MQ=255)
                                                                                                                     ggCTCGATCAAGCCGGGTGTGCCGGTTACCTATCGTGAGGTTACGGTGGGCAAGGTGACAGGCTTCg                                                                                        <  7:197828/67‑1 (MQ=255)
                                                                                                                     ggCTCGATCAAGCCGGGTGTGCCGGTTACCTATCGTGAGGTTACGGTGGGCAAGGTGACAGGCTTCg                                                                                        >  8:197828/1‑67 (MQ=255)
                                                                                                                       cTCGATCAAGCCGGGTGTGCCGGTTACCTATCGTGAGGTTACGGTgg                                                                                                          <  3:158545/47‑1 (MQ=255)
                                                                                                                       cTCGATCAAGCCGGGTGTGCCGGTTACCTATCGTGAGGTTACGGTgg                                                                                                          >  4:158545/1‑47 (MQ=255)
                                                                                                                             cAAGCCGGGTGTGCCGGTTACCTATCGTGAGGTTACGGTGGGCAAGGTGACAGGCTTCGAGCTGGGCCAGAGCGCTGACCGCGTGCTGATTCATATCCTCATCGAGCCGCGCTATGCCGCCTTGGTGCGCAGCg             >  8:112026/1‑134 (MQ=255)
                                                                                                                              aaGCCGGGTGTGCCGGTTACCTATCGTGAGGTTACGGTGGGCAAGGTGACAGGCTTCGAGCTGGGCCAGAGCGCTGACCGCGTGCTGATTCATATCCTCATCGAGCCGCGCTATGCCGCCTTGGTGCGCAGCg             >  8:114325/1‑133 (MQ=255)
                                                                                                                              aaGCCGGGGGGGCCGGTTACCTATCGTGAGGTTACGGTGGGCAAGGTGACAGGCTTCGAGCTGGGCCAGAGCGCTGACCGCGTGCTGATTCATATCCTCATCGAGCCGCGCTATGCCGCCTTGGTGCGCAGCGGTa          <  4:57065/136‑1 (MQ=255)
                                                                                                                               aGCCGGGTGTGCCGGTTACCTATCGTGAGGTTACGGTGGGCAAGGTGACAGGCTTCGAGCTGGGCCAGAGCGCTGCCCGCGTGCTGATTCATATCCTCATCGAGCCGCGCTATGCCGCCTTGGTGCGCAGCGGTa          >  7:103995/1‑135 (MQ=255)
                                                                                                                                      tgtgCCGGTTACCTATCGTGAGGTTACGGTGGGCAAGGTGACAGGCTTCGAGCTGGGCCAGAGCGCTGACCGCGTGCTGATTCATATCCTCATCGAGCCGCGCTATGCCGCCTTGGTGCGCAGCGGTAGCCGCTTc  <  4:46756/136‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                       
GTGCAGCCAGCAGCCAAGAACCGTGGGCCACAGCGCGATTTCATCGCCCTGGCCGAAGCACCGGAGGTAGCAGGGCCAGAGGTCGGCCTGCCATTGACGCTCAGTGCGCCACGCCGTGGCTCGATCAAGCCGGGTGTGCCGGTTACCTATCGTGAGGTTACGGTGGGCAAGGTGACAGGCTTCGAGCTGGGCCAGAGCGCTGACCGCGTGCTGATTCATATCCTCATCGAGCCGCGCTATGCCGCCTTGGTGCGCAGCGGTAGCCGCTTC  >  KT2440/699386‑699655

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: