Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 3,039,492 Δ1 bp 5.7% coding (68/786 nt) pstB3 → Phosphate import ATP‑binding protein PstB 3

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24403,039,4920C. 5.7% 212.0 / 6.7 70coding (68/786 nt)pstB3Phosphate import ATP‑binding protein PstB 3
Reads supporting (aligned to +/- strand):  ref base C (31/34);  new base . (2/2);  total (34/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCCAAGTATTGAGAAAGGGAGCATCCAACTTGAACGTATCCACTGCGCAAAGAGCCGCTCCCATGGTCAGCGAAGCCCCCATCGTCATGGACTGCAAGCTCGACAAGATTTTCTACGGTAACTTCATGGCCGTGCGTGACAGCCATGTGCCGATCAGGAAAAACGAGATCACCGGTTTCATCGGCCCTTCGGGCTGCGGCAAGAGTACCGTGCTGCGTAGCTTGAACCGTATGAATGACCTGGTGAAGGGCTTCCGCTTCGAAG  >  KT2440/3039363‑3039626
                                                                                                                                 |                                                                                                                                      
cccAAGTATTGAGAAAGGGAGCATCCAACTTGAACGTATCCACTGCGCAAAGAGCCGCTCCCATGGTCAGCGAAGCCCCCATCGTCATGGACTGCAAGCTCGACAAGATTTTCTACGGTAACTTCATGGCcgtgcg                                                                                                                                  <  3:182344/136‑1 (MQ=255)
cccAAGTATTGAGAAAGGGAGCATCCAACTTGAACGTATCCACTGCGCAAAGAGCCGCTCCCATGGTCAGCGAAGCCCACATCGTCATGGACTGCAAGCTCGACAAGATTTTCTACGGTAACTTCATGGCcgtgcg                                                                                                                                  <  3:129721/136‑1 (MQ=255)
 ccAAGTATTGAGAAAGGGAGCATCCAACTTGAACGTATCCACTGCGCAAAGAGCCGCTCCCATGGTCAGCGAAGCCCCCATCGTCATGGACTGCAAGCTCGACAAGATTTTCTACGGTAACTTCATGGCcgtgcg                                                                                                                                  <  7:324690/135‑1 (MQ=255)
  cAAGTATTGAGAAAGGGAGCATCCAACTTGAACGTATCCACTGCGCAAAGAGCCGCTCCCATGGTCAGCGAAGCCCCCATCGTCATGGACTGCAAGCTCGACAAGATTTTCTACGGTAACTTCATGGCcgtgcgt                                                                                                                                 >  2:28161/1‑135 (MQ=255)
  cAAGTATTGAGAAAGGGAGCATCCAACTTGAACGTATCCACTGCGCAAAGAGCCGCTCCCATGGTCAGCGAAGCCCCCATCGTCATGGACTGCAAGCTCGACAAGATTTTCTACGGTAACTTCATGGCcgtgcg                                                                                                                                  >  2:82368/1‑134 (MQ=255)
  cAAGTATTGAGAAAGGGAGCATCCAACTTGAACGTATCCACTGCGCAAAGAGCCGCTCCCATGGTCAGCGAAGCCCCCATCGTCATGGACTGCAAGCTCGACAAGATTTTCTACGGTAACTTCATGGCcgtgcg                                                                                                                                  >  1:42929/1‑134 (MQ=255)
  cAAGTATTGAGAAAGGGAGCATCCAACTTGAACGTATCAACTGCGCAAAGAGCCGCTCCAATGGTCAGCGAAGCCCCCATCGTCATGGACTGCAAGCTCGACAAGATTTTCTACGGTAACTTCATGGCcgtgag                                                                                                                                  >  2:35559/1‑132 (MQ=255)
             aaaGGGAGCATCCAACTTGAACGTATCCACTGCGCAAAGAGCCGCTCCCATGGTCAGCGAAGCCCCCATCGTCATGGACTGCAAGCTCGACAAGATTTTCTACGGTAACTTCATGGCCGTGCGTGACAGCCAtgtg                                                                                                                     >  3:234593/1‑136 (MQ=255)
             aaaGGGAGCATCCAACTTGAACGTATCCACTGCGCAAAGAGCCGCTCCCATGGTCAGCGAAGCCCCCATCGTCATGGACTGCAAGCTCGACAAGATTTTCTACGGTAACTTCATGGCCGTGCGTGACAGCCAtgt                                                                                                                      >  2:57820/1‑135 (MQ=255)
                gggAGCATCCAACTTGAACGTATCCACTGCGCAAAGAGCCGCTCCCATGGTCAGCGAAGCCCCCATCGTCATGGACTGCAAGCTCGACAAGATTTTCTACGGTAACTTCATGGCCGTGCGTGACAGCCATGTGcc                                                                                                                   <  1:71440/135‑1 (MQ=255)
                   aGCATCCAACTTGAACGTATCCACTGCGCAAAGAGCCGCTCCCATGGTCAGCGAAGCCCCCATCGTCATGGACTGCAAGCTCGACAAGATTTTCTACGGTAACTTCATGGCCGTGCGTGACAGCCATGTGCCGa                                                                                                                 >  8:109868/1‑134 (MQ=255)
                          aaCTTGAACGTATCCACTGCGCAAAGAGCCGCTCCCATGGTCAGCGAAGCCCCCATCGTCATGGACTGCAAGCTCGACAAGATTTTCTACGGTAACTTCATGGCCGTGCGTGACAGCCATGTGCCGATCAGGaa                                                                                                          >  1:150536/1‑134 (MQ=255)
                          aaCTTGAACGTATCCACTGCGCAAAGAGCCGCTCCCATGGTCAGCGAAGCCCCCATCGTCATGGACTGCAAGCTCGACAAGATTTTCTACGGTAACTTCATGGCCGTGCGTGACAGCCATGTGCCGATCAGGaa                                                                                                          >  5:294999/1‑134 (MQ=255)
                                   gTATCCACTGCGCAAAGAGCCGCTCCCATGGTCAGCGAAGCCCCCATCGTCATGGACTGCAAGCTCGACAAGATTTTCTACGGTAACTTCATGGCCGTGCGTGACAGCCATGTGCCGATCAGGAAAAACGAGATc                                                                                                >  8:186889/1‑135 (MQ=255)
                                      tCCACTGCGCAAAGAGCCGCTCCCATGGTCAGCGAAGCCCCCATCGTCATGGACTGCAAGCTCGACAAGATTTTCTACGGTAACTTCATGGCCGTGCGTGACAGCCATGTGCCGATCAGGAAAAACGAGATCAcc                                                                                             <  3:40601/135‑1 (MQ=255)
                                         aCTGCGCAAAGAGCCGCTCCCATGGTCAGCGAAGCCCCCATCGTCATGGACTGCAAGCTCGACAAGATTTTCTACGGTAACTTCATGGCCGTGCGTGACAGCCATGTGCCGATCAGGAAAAACGAGATCACCGGt                                                                                          >  1:270986/1‑135 (MQ=255)
                                             cgcAAAGAGCCGCTCCCATGGTCAGCGAAGCCCCCATCGTCATGGACTGCAAGCTCGACAAGATTTTCTACGGTAACTTCATGGCCGTGCGTGACAGCCATGTGCCGATCAGGAAAAACGAGATCACCGGTTTCa                                                                                      <  4:234593/135‑1 (MQ=255)
                                              gcAAAGAGCCGCTCCCATGGTCAGCGAAGCCCCCATCGTCATGGACTGCAAGCTCGACAAGATTTTCTACGGTAACTTCATGGCCGTGCGTGACAGCCATGTGCCGATCAGGAAAAACGAGATCACCGGTTTCAt                                                                                     >  8:78747/1‑135 (MQ=255)
                                                aaaGAGCCGCTCCCATGGTCAGCGAAGCCCCCATCGTCATGGACTGCAAGCTCGACAAGATTTTCTACGGTAACTTCATGGCCGTGCGTGACAGCCATGTGCCGATCAGGAAAAACGAGATCACCGGTTTCATCg                                                                                   >  6:39164/1‑135 (MQ=255)
                                                aaaGAGCCGCTCCCATGGTCAGCGAAGCCCCCATCGTCATGGACTGCAAGCTCGACAAGATTTTCTACGGTAACTTCATGGCCGTGCGTGACAGCCATGTGCCGATCAGGAAAAACGAGATCACCGGTTTCATCg                                                                                   >  7:33582/1‑135 (MQ=255)
                                                 aaGAGCCGCTCCCATGGTCAGCGAAGCCCCCATCGTCATGGACTGCAAGCTCGACAAGATTTTCTACGGTAACTTCATGGCCGTGCGTGACAGCCATGTGCCGATCAGGAAAAACGAGATCACCGGTTTCATCgg                                                                                  >  8:41715/1‑135 (MQ=255)
                                                        gCTCCCATGGTCAGCGAAGCCCCCATCGTCATGGACTGCAAGCTCGACAAGATTTTCTACGGTAACTTCATGGCCGTGCGTGACAGCCATGTGCCGATCAGGAAAAACGAGATCACCGGTTTCATCGGCCCTTCgg                                                                          <  4:277195/136‑1 (MQ=255)
                                                        gCTCCCATGGTCAGCGAAGCCCCCATCGTCATGGACTGCAAGCTCGACAAGATTTTCTACGGTAACTTCATGGCCGTGCGTGACAGCCATGTGCCGATCAGGAAAAACGAGATCACCGGTTTCATCGGCCCTTCg                                                                           <  8:128080/135‑1 (MQ=255)
                                                          tCCCATGGTCAGCGAAGCCCCCATCGTCATGGACTGCAAGCTCGACAAGATTTTCTACGGTAACTTCATGGCCGTGCGTGACAGCCATGTGCCGATCAGGAAAAAc                                                                                                      >  7:331653/1‑106 (MQ=255)
                                                          tCCCATGGTCAGCGAAGCCCCCATCGTCATGGACTGCAAGCTCGACAAGATTTTCTACGGTAACTTCATGGCCGTGCGTGACAGCCATGTGCCGATCAGGAAAAAc                                                                                                      <  8:331653/106‑1 (MQ=255)
                                                          tCCCATGGTCAGCGAAGCCCCCATCGTCATGGACTGCAAGCTCGACAAGATTTTCTACGGTAACTTCATGGCCGTGCGTGACAGCCATGTGCCGATCAGGAAAAACGAGATCACCGGTTTCATCGGCCCTTCggg                                                                         >  8:144326/1‑135 (MQ=255)
                                                            ccATGGTCAGCGAAGCCCCCATCGTCATGGACTGCAAGCTCGACAAGATTTTCTACGGTAACTTCATGGCCGTGCGTGACAGCCATGTGCCGATCAGGAAAAACGAGATCACCGGTTTCATCGGCCCTTCGGGCt                                                                       >  2:46163/1‑135 (MQ=255)
                                                            ccATGGTCAGCGAAGCCCCCATCGTCATGGACTGCAAGCTCGACAAGATTTTCTACGGTAACTTCATGGCCGTGCGTGACAGCCATGTGCCGATCAGGAAAAACGAGATCACCGGTTTCATCGGCCCTTCGGGCTg                                                                      >  3:311766/1‑136 (MQ=255)
                                                             cATGGTCAGCGAAGCCCCCATCGTCATGGACTGCAAGCTCGACAAGATTTTCTACGGTAACTTCATGGCCGTGCGTGACAGCCATGTGCCGATCAGTAAAAACGAGATCACCGGTTTCATCGGCCCTTCGGGc                                                                        <  7:344377/133‑1 (MQ=255)
                                                             cATGGTCAGCGAAGCCCCCATCGTCATGGACTGCAAGCTCGACAAGATTTTCTACGGTAACTTCATGGCCGTGCGTGACAGCCATGTGCCGATCAGGAAAAACGAGATCACCGGTTTCATCGGCCCTTCGGGc                                                                        <  6:294999/133‑1 (MQ=255)
                                                             cATGGTCAGCGAAGCCCCCATCGTCATGGACTGCAAGCTCGACAAGATTTTCTACGGTAACTTCATGGCCGTGCGTGACAGCCATGTGCCGATCAGGAAAAACGAGATCACCGGTTTCATCGGCCCTTCGGGc                                                                        <  2:150536/133‑1 (MQ=255)
                                                                gggcAGCGAAGCCCCCATCGTCATGGACTGCAAGCTCGACAAGATTTTCTACGGTAACTTCATGGCCGTGCGTGACAGCCATGTGCCGATCAGGAAAAACGAGATCACCGGTTTCATCGGCCCTTCGGGCTGCgg                                                                   <  2:4653/132‑1 (MQ=255)
                                                                ggTCAGCGAAGCCCCCATCGTCATGGACTGCAAGCTCGACAAGATTTTCTACGGTAACTTCATGGCCGTGCGTGACAGCCATGTGCCGATCAGGAAAAACGAGATCACCGGTTTCATCGGCCCTTCGGGCTGCgg                                                                   <  7:230987/135‑1 (MQ=255)
                                                                 gTCAGCGAAGCCCCCATCGTCATGGACTGCAAGCTCGACAAGATTTTCTACGGTAACTTCATGGCCGTGCGTGACAGCCATGTGCCGATCAGGAAAAACGCGATCACCGGTTTCATCGGCCCTTCGGGCTGCGGc                                                                  >  4:361438/1‑135 (MQ=255)
                                                                     gCGAAGCCCCCATCGTCATGGACTGCAAGCTCGACAAGATTTTCTACGGTAACTTCATGGCCGTGCGTGACAGCCATGTGCCGATCAGGAAAAACGAGATCACCGGTTTCATCGGCCCTTCGGGCTGCGGCAaga                                                              <  8:341705/135‑1 (MQ=255)
                                                                      cGAAGCACCCATCGTCATGGACTGCAAGCTCGACAAGATTTTCTACGGTAACTTCATGGCCGTGCGTGACAGCCATGTGCCGATCAGGAAAAACGAGATCACCGGTTTCATCGGCCCTTCGGGCTGCGGCAAGAGt                                                            <  3:217990/136‑1 (MQ=255)
                                                                       gAAGCCCCCATCGTCATGGACTGCAAGCTCGACAAGATTTTCTACGGTAACTTCATGGCCGTGCGTGACAGCCATGTGCCGATCAGGAAAAACga                                                                                                    <  7:314115/95‑1 (MQ=255)
                                                                       gAAGCCCCCATCGTCATGGACTGCAAGCTCGACAAGATTTTCTACGGTAACTTCATGGCCGTGCGTGACAGCCATGTGCCGATCAGGAAAAACga                                                                                                    >  8:314115/1‑95 (MQ=255)
                                                                       gAAGCCCCCATCGTCATGGACTGCAAGCTCGACAAGATTTTCTACGGTAACTTCATGGCCGTGCGTGACAGCCATGTGCCGATCAGGAAAAACGAGATCACCGGTTTCATCGGCCCTTCGGGCTGCGGCAAGAGt                                                            >  7:281721/1‑135 (MQ=255)
                                                                       gAAGCCCCCATCGTCATGGACTGCAAGCTCGACAAGATTTTCTACGGTAACTTCATGGCCGTGCGTGACAGCCATGTGCCGATCAGGAAAAACGAGATCACCGGTTTCATCGGCCCTTCGGGCTGCGGCAAGAGTa                                                           >  4:278085/1‑136 (MQ=255)
                                                                         aGCCCCCATCGTCATGGACTGCAAGCTCGACAAGATTTTCTACGGTAACTTCATGGCCGTGCGTGACAGCCATGTGCCGATCAGGAAAAACGAGATCACCGGTTTCATCGGCCCTTCGGGCTGCGGCAAGAGTAc                                                          >  1:334987/1‑135 (MQ=255)
                                                                          gCCCCCATCGTCATGGACTGCAAGCTCGACAAGATTTTCTACGGTAACTTCATGGCCGTGCGTGACAGCCATGTGCCGATCAGGAAAAACGAGATCACCGGTTTCATCGGCCCTTCGGGCTGCGGCAAGAGTAcc                                                         <  7:109868/135‑1 (MQ=255)
                                                                              ccATCGTCATGGACTGCAAGCTCGACAAGATTTTCTACGGTAACTTCATGGCCGTGCGTGACAGCCATGTGCCGATCAGGAAAAACGAGATCACCGGTCTCATCGGCCCTTCGGGCTGCGGCAAGAGTACCGtgc                                                     <  2:42929/135‑1 (MQ=255)
                                                                               cATCGTCATGGACTGCAAGCTCGACAAGATTTTCTACGGTAACTTCATGGCCGTGCGTGACAGCCATGTGCCGATCAGGAAAAACGAGATCACCGGTTTCATCGGCCCTTCGGGCTGCGGCAAGAGTACCGtgct                                                    <  7:413/135‑1 (MQ=255)
                                                                                   gTCATGGACTGCAAGCTCGACAAGATTTTCTACGGTAACTTCATGGCCGTGCGTGACAGCCATGTGCCGATCAGGAAAAACgaga                                                                                                  >  4:285738/1‑85 (MQ=255)
                                                                                   gTCATGGACTGCAAGCTCGACAAGATTTTCTACGGTAACTTCATGGCCGTGCGTGACAGCCATGTGCCGATCAGGAAAAACgaga                                                                                                  <  3:285738/85‑1 (MQ=255)
                                                                                        ggACTGCAAGCTCGACAAGATTTTCTACGGTAACTTCATGGcc                                                                                                                                       >  1:161576/1‑43 (MQ=255)
                                                                                        ggACTGCAAGCTCGACAAGATTTTCTACGGTAACTTCATGGcc                                                                                                                                       <  2:161576/43‑1 (MQ=255)
                                                                                        ggACTGCAAGCTCGACAAGATTTTCTACGGTAACTTCATGGCCGTGCGTGACAGCCATGTGCCGATCAGGAAAAACGAGATCACCGGTTTCATCGGCCCTTCGGGCTGCGGCAAGAGTACCGTGCTGCGTAGCtt                                           <  1:82368/135‑1 (MQ=255)
                                                                                         gACTGCAAGCTCGACAAGATTTTCTACGGTAACTTCATGGCCGTGCGTGACAGCCATGTGCCGATCAGGAAAAACGAGATCCCCGGTTTCATCGGCCCTTCGGGCt                                                                       >  6:285837/1‑106 (MQ=255)
                                                                                         gACTGCAAGCTCGACAAGATTTTCTACGGTAACTTCATGGCCGTGCGTGACAGCCATGTGCCGATCAGGAAAAACGAGATCACCGGTTTCATCGGCCCTTCGGGCt                                                                       <  5:285837/106‑1 (MQ=255)
                                                                                         gACTGCAAGCTCGACAAGATTTTCTACGGTAACTTCATGGCCGTGCGTGACAGCCATGTGCCGATCAGGAAAAACGAGATCACCGGTTTCATCGGCCCTTCGGGCTGCGGCAAGAGTACCGTGCTGCGTAGCtt                                           <  4:247180/134‑1 (MQ=255)
                                                                                           cTGCAAGCTCGACAAGATTTTCTACGGTAACTTCATGGCCGTGCGTGACAGCCATGTGCCGATCAGGAAAAACGAGATCACCGGTATCATCGGCCCTTCGGGCTGCGGCAAGAGTACCGTGCTGCGTAGCTTGaa                                        <  2:334987/135‑1 (MQ=255)
                                                                                             gCAAGCTCGACAAGATTTTCTACGGTAACTTCATGGACGTGCGTGACAGCCATCTGCCGATCAGGAAACACGAGAGCACCGGTTTCATCGGCCCTTCGTGCTGCCGCAAGAGTACCGAGCTGGGTGGCTTGAAcc                                      >  1:256917/1‑135 (MQ=255)
                                                                                                aGCTCGACAAGATTTTCTACGGTAACTTCATGGCCGTGCGTGACAGCCATGTGCCGATCAGGAAAAACGAGATCACCGGTTTCATCGGCCCTTCGGGCTGCGGCAAGAGTACCGTGCTGCGTAGCTTGAACCGTa                                   <  6:149493/135‑1 (MQ=255)
                                                                                                 gCTCGACAAGATTTTCTACGGTAACTTCATGGCCGTGCGTGACAGCCATGTGCCGATCAGGAAAAACGAGATCACCGGTTTCATCGGCCCTTCGGGCTGCGGCAAGAGTACCGtgc                                                     >  4:168464/1‑116 (MQ=255)
                                                                                                 gCTCGACAAGATTTTCTACGGTAACTTCATGGCCGTGCGTGACAGCCATGTGCCGATCAGGAAAAACGAGATCACCGGTTTCATCGGCCCTTCGGGCTGCGGCAAGAGTACCGtgc                                                     <  3:168464/116‑1 (MQ=255)
                                                                                                 gCTCGACAAGATTTTCTACGGTAACTTCATGGCCGTGCGTGACAGCCATGTGCCGATCAGGAAAAACGAGATCACCGGTTTCATCGGCCCTTCGGGCTGCGGCAAGAGTACCGTGCTGCGTAGCTTGAACCGTa                                   >  6:328754/1‑134 (MQ=255)
                                                                                                  cTCGACAAGATTTTCTACGGTAACTTCATGGCCGTGCGTGACAGCCATGTGCCGATCAGGAAAAACGAGATCACCGGTTTCATCGGCCCTTCGGGCTGCGGCAAGAGTACCGTGCTGCGTAGCTTGAACCGTatg                                 <  7:144326/135‑1 (MQ=255)
                                                                                                     gagaaGATTTTCTACGGTAACTTGATGGCGGTGGGTGACAGCCATGTGCCGATCAGGAAACACGAGATCACCGGTTTCATCGGCCCTTCGGGCTGCGGCAAGAGTACCGTGCTGCGTAGCTTGAACCGTatgaat                              <  1:57820/132‑1 (MQ=255)
                                                                                                     gaaaaGATTTTCTACGGTAACTTCATGGCCGTGCGTGACAGCCATGTGCCGATCAGGAAAAACGAGATCACCGGTTTCATCGGCCCTTCGGGCTGCGGCAAGAGTACCGTGCTGCGTAGCTTGAACCGTatgaat                              <  7:41715/132‑1 (MQ=255)
                                                                                                      aCAAGATTTTCTACGGTAACTTCATGGCCGTGCGTGACAGCCATGTGCCGATCAGGAAAAACGAGATCACCGGTTTCATCGGCCCTTCGGGCTGCGGCAAGAGTACCGTGCTGCGTAGCTTGAACCGTatgaat                              <  7:66803/134‑1 (MQ=255)
                                                                                                         aGATTTTCTACGGTAACTTCATGGCCGTGCGTGACAGCCATGTGCCGATCAGGAAAAACGAGATCACCGGTTTCATCGGCCCTTCGGGCTGCGGCAAGAGTACCGTGCTGCGTAGCTTGAACCGTATGAATGAcc                          >  5:345421/1‑135 (MQ=255)
                                                                                                         aGATTTTCTACGGTAACTTCATGGCGTGCGTGACAGCCATGTGCCGATCAGGAAAAACGAGATCACCGGTTTCATCGGCCCTTCGGGCTGCGGCAAGAGTACCGTGCTGCGTAGCTTGAACCGTatgaat                              <  8:204080/130‑1 (MQ=255)
                                                                                                         aGATTTTCTACGGTAACTTCATGGCGTGCGTGACAGCCATGTGCCGATCAGGAAAAACGAGATCACCGGTTTCATCGGCCCTTCGGGCTGCGGCAAGAGTACCGTGCTGCGTAGCTTGAACCGTatgaat                              <  8:209355/130‑1 (MQ=255)
                                                                                                         aGATTTTCTACGGTAACTTCATGGCGTGCGTGACAGCCATGTGCCGATCAGGAAAAACGAGATCACCGGTTTCATCGGCCCTTCGGGCTGCGGCAAGAGTACCGTGCTGCGTAGCTTGAACCGTatgaat                              >  7:204080/1‑130 (MQ=255)
                                                                                                         aGATTTTCTACGGTAACTTCATGGCGTGCGTGACAGCCATGTGCCGATCAGGAAAAACGAGATCACCGGTTTCATCGGCCCTTCGGGCTGCGGCAAGAGTACCGTGCTGCGTAGCTTGAACCGTatgaat                              >  7:209355/1‑130 (MQ=255)
                                                                                                              ttCTACGGTAACTTCATGGCCGTGCGTGACAGCCATGTGCCGATCAGGAAAAACGAGATCACCGGTTTCATCGGCCCTTCGGGCTGCGGCAAGAGTACCGTGCTGCGTAGCTTGAACCGTATGAATGACCTGGTg                     <  4:336369/135‑1 (MQ=255)
                                                                                                               tCTACGGTAACTTCATGGCCGTGCGTGACAGCCATGTGCCGATCAGGAAAAACGAGATCACCGGTTTCATCGGCCCTTCGGGCTGCGGCAAGAGTACCGTGCTGCGTAGCTTGAACCGTATGAATGACCTGGTg                     <  8:114048/134‑1 (MQ=255)
                                                                                                                  aCGGTAACTTCATGGCCGTGCGTGACAGCCATGTGCCGATCAGGAAAAACGAGATCACCGGTTTCATCGGCCCTTCGGGCTGCGGCAAGAGTACCGTGCTGCGTAGCTTGAACCGTatgaat                              <  3:131893/122‑1 (MQ=255)
                                                                                                                  aCGGTAACTTCATGGCCGTGCGTGACAGCCATGTGCCGATCAGGAAAAACGAGATCACCGGTTTCATCGGCCCTTCGGGCTGCGGCAAGAGTACCGTGCTGCGTAGCTTGAACCGTatgaat                              >  4:131893/1‑122 (MQ=255)
                                                                                                                            cATGGCCGTGCGTGACAGCCATGTGCCGATCAGGAAAAACGAGATCACCGGTTTCATCGGCCCTTCGGGCTGCGGCAAGAGTACCGTGCTGCGTAGCTTGAACCGTATGAATGACCTGGTGAAGGGCTTCCGCtt       >  1:333084/1‑135 (MQ=255)
                                                                                                                                 ccGTGCGTGACAGCCATGTGCCGATCAGGAAAAACGAGATCACCGGTTTCATCGGCCCTTCGGGCTGCGGCAAGAGTACCGTGCTGCGTAGCTTGAACCGTATGAATGACCTGGTGAAGGGCTTCCGCTTCGAAg  <  2:270537/135‑1 (MQ=255)
                                                                                                                                 |                                                                                                                                      
CCCAAGTATTGAGAAAGGGAGCATCCAACTTGAACGTATCCACTGCGCAAAGAGCCGCTCCCATGGTCAGCGAAGCCCCCATCGTCATGGACTGCAAGCTCGACAAGATTTTCTACGGTAACTTCATGGCCGTGCGTGACAGCCATGTGCCGATCAGGAAAAACGAGATCACCGGTTTCATCGGCCCTTCGGGCTGCGGCAAGAGTACCGTGCTGCGTAGCTTGAACCGTATGAATGACCTGGTGAAGGGCTTCCGCTTCGAAG  >  KT2440/3039363‑3039626

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: