Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	KT2440	5,844,716	A→C	22.9%	E529A (GAG→GCG)	ilvD →	Dihydroxy‑acid dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	5,844,716	0	A	C	22.9%	91.7 / 4.2	67	E529A (GAG→GCG)	ilvD	Dihydroxy‑acid dehydratase
Reads supporting (aligned to +/- strand):  ref base A (32/17);  new base C (6/12);  total (38/29)
Fisher's exact test for biased strand distribution p-value = 2.67e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCCGGGCATGCAAGAAATGCTCTACCCGACCTCGTACCTCAAGTCCAAAGGCCTGGGCAAGGCCTGCGCCCTGCTCACCGACGGTCGCTTCTCGGGCGGTACCTCGGGCCTGTCGATCGGCCACGCCTCGCCAGAGGCCGCGGCGGGCGGCGCCATCGGCCTGGTGCGCGACGGCGACAAGGTACTGATCGACATCCCTAACCGCTCGATCAACCTGCTGGTCAGCGATGAAGAGCTGGCGCAACGCCGTGTCGAGCAGGACAAGAA  >  KT2440/5844582‑5844848                                                                                                                                       |                                                                                                                                     cccGGGCATGCAAGAAATGCTCTACCCGACCTCGTACCTCAAGTCCAAAGGCCTGGGCAAGGCCTGCGCCCTGCTCACCGACGGTCGCTTCTCGGGCGGTACCTCGGGCCTGTCGATCGGCCACGCCTCGCCaga                                                                                                                                      >  2:98131/1‑135 (MQ=255) cccGGGCATGCAAGAAATGCTCTACCCGACCTCGTACCTCAAGTCCAAAGGCCTGGGCAAGGCCTGCGCCCTGCTCACCGACGGTCGCTTCTCGGGCGGTACCTCGGGCCTGTCGATCGGCCACGCCTCGCCaga                                                                                                                                      >  1:48819/1‑135 (MQ=255)   cGGGCATGCAAGAAATGCTCTACCCGACCTCGTACCTCAAGTCCAAAGGCCTGGGCAAGGCCTGCGCCCTGCTCCCCGACTGTCGCTTCGCGGGCGGTACCTCGGGCCTGTCGATCGGCCACGCCGCGCCAGAgg                                                                                                                                    >  2:121194/1‑135 (MQ=255)   cGGGCATGCAAGAAATGCTCTACCCGACCTCGTACCTCAAGTCCAAAGGCCTGGGCAAGGCCTGCGCCCTGCTCACCGACGGTCGCTTCTCGGGCGGTACCTCGGGCCTGTCGATCGGCCACGCCTCGCCAGAgg                                                                                                                                    >  8:44549/1‑135 (MQ=255)         tGCAAGAAATGCTCTACCCGACCTCGTACCTCAAGTCCAAAGGCCTGGGCAAGGCCTGCGCCCTGCTCACCGACGGTCGCTTCTCGGGCGGTACCTCGGGCCTGTCGATCGGCCACGCCTCGCCAGAGGCCgcgg                                                                                                                              >  2:277652/1‑135 (MQ=255)          gCAAGAAATGCTCTACCCGACCTCGGACCTCAAGTCCAAAGGCCTGGGCAAGGCCTGCGCCCTGCTCAGCGCCGGTCGCTTCTCGGGCGGTCCCTCAGGCCTGTCGATAGGCCACGCCTCGCCAGAGGCCgcggc                                                                                                                             >  7:2498/1‑135 (MQ=255)              gAAATGCTCTACCCGACCTCGTACCTCAAGTCCAAAGGCCTGGGCAAGGCCTGCGCCCTGCTCACCGACGGTCGCTTCTCGGGCGGTACCTCGGGCCTGTCGATCGGCCACGCCTCGCCAGCGGCCGCggcgggc                                                                                                                         >  6:265387/1‑135 (MQ=255)              gAAATGCTCTACCCGACCTCGTACCTCAAGTCCAAAGGCCTGGGCAAGGCCTGCGCCCTGCTCACCGACGGTCGCTTCTCGGGCGGTACCTCGGGCCTGTCGATCGGCCACGCCTCGCCAGAGGCCGCggcgggc                                                                                                                         >  6:169515/1‑135 (MQ=255)                       tACCCGACCTCGTACCTCAAGTCCAAAGGCCTGGGCAAGGCCTGCGCCCTGCTCACCGACGGTCGCTTCTCGGGCGGTACCTCGGGCCTGTCGATCGGCCACGCCTCGCCAGCGGCCGCGGCGGGCGGCGCCaac                                                                                                                >  6:354994/1‑133 (MQ=255)                       tACCCGACCTCGTACCTCAAGTCCAAAGGCCTGGGCAAGGCCTGCGCCCTGCTCACCGACGGTCGCTTCTCGGGCGGTACCTCGGGCCTGTCGATCGGCCACGCCTCGCCAGCGGCCGCGGCGGGCGGCGCCAta                                                                                                                >  8:296067/1‑134 (MQ=255)                         cccGACCTCGTACCTCAAGTCCAAAGGCCTGGGCAAGGCCTGCGCCCTGCTCACCGACGGTCGCTTCTCGGGCGGTACCTCGGGCCTGTCGATCGGCCACGCCTCGCCAGAGGCCGCGGCGCGCGGCGcccacgc                                                                                                              >  1:332203/1‑130 (MQ=255)                            gACCTCGTACCTCAAGTCCAAAGGCCTGGGCAAGGCCTGCGCCCTGCTCACCGACGGTCGCTTCTCGGGCGGTACCTCGGGCCTGTCGATCGGCCACGCCTCTCCAGAGGCCGCGGCGGGCGGCGCCATCGGCCt                                                                                                           >  2:303017/1‑135 (MQ=255)                                 cGTACCTCAAGTCCAAAGGCCTGGGCAAGGCCTGCGCCCTGCTCACCGACGGTCGCTTCTCGGGCGGTACCTCGGGCCTGTCGATCGGCCACGCCTCGCCAGAGGCCCCGGCGGGCGGCGCCCTCGGCCGGGTgc                                                                                                      >  5:84225/1‑135 (MQ=255)                                 cGTACCTCAAGTCCAAAGGCCTGGGCAAGGCCTGCGCCCTGCTCACCGACGGTCGCTTCTCGGGCGGTACCTCGGGCCTGTCGATCGGCCACGCCTCGCCAGACGCCGCGGCGGGCGGCGCCAACGGCCGGGTgc                                                                                                      >  2:332977/1‑135 (MQ=255)                                 cGTACCTCAAGTCCAAAGGCCTGGGCAAGGCCTGCGCCCTGCTCACCGACGGTCGCTTCTCGGGCGGTACCTCGGGCCTGTCGATCGGCCACCCCGCGCCACAGGCCCCGGCGGGCGGCGCCCACCGCCCGGTgc                                                                                                      >  2:170914/1‑135 (MQ=255)                                 cGTACCTCAAGTCCAAAGGCCTGGGCAAGGCCTGCGCCCTGCTCACCGACGGTCGCTTCTCGGGCGGTACCTAGGGCCTGTCGATCGGCCCCGCCTCGCCAGAGGCCGCGGCGGGCGGCGCCATCGGCCTGGTgcg                                                                                                     >  3:361059/1‑136 (MQ=255)                                 cGTACCTCAACGCCAACGGCCTGGGCAAGGCCTGCGCCCTGCTCACCGACGGTCGCGTCGCGGGCGGTACCTCGGGCCTGGCGAGCGGCCACGCCTCGCCAGAGGCCGCGGCGGGCGGCGCCATCGGCCTGGTgcg                                                                                                     <  3:302526/136‑1 (MQ=255)                                    aCCTCAAGTCCAAAGGCCTGGGCAAGGCCTGCGCCCTGCTCACCGACGGTCGCTTCTCGGGCGGTACCTCGGGCCTGTCGATCGGCCACGCCTCGCCAGAGGCCGCGGCGGGCGGCGCCATCGGCCGGGTGcgcg                                                                                                   >  8:69741/1‑135 (MQ=255)                                     ccTCACGCCCAACGCCCGGGGCACGGCCTGCGCCCTGCTCACCGAGGGGCGCTTCGCGGGCGGTACCTCGGGCCTGTCGATCGGCCACGCCCCGCCAGAGGCCGCGGCGGGCGGCGCCATCGGCCTGGTGCGCGa                                                                                                  <  1:7777/135‑1 (MQ=255)                                      cTCAAGTCCAAAGGCCTGGGCAAGGCCTGCGCCCTGCTCACCGACGGTCGCTTCTCGGGCGGTACCTCGGGCCAGTCGATCGGCCACGCCTCGCCAGAGGCCGCGGCGGGCGGCGCGCTCGGCCTGGTGCGCCAc                                                                                                 >  1:211810/1‑135 (MQ=255)                                         aaGGCCAACGGCCGGGGCACGGCCTGCGCCCTGCGCACCGAGGGTCGCTTCTCGGGCGGTACCGCGGGCGTGTCGATCGGCCACGCCTCGCCAGAGGCCGCGGCGGGCGGCGCCATCGGCCTGGTGCGCGACgg                                                                                               <  3:365220/134‑1 (MQ=255)                                            tCCAAAGGCCTGGGCAAGGCCTGCGCCCTGCTCACCGACGGTCGCTTCTCGGGCGGTACCTCGGGCCTGTCGATCGGCCACGCCTCGCCAGCGGCCGCGGCGGGCGGCGCCCACCGCCCGGGGCgcggcggcgc                                                                                            >  5:365053/1‑133 (MQ=255)                                              cAAAGGCCTGGGCAAGGCCTGCGCCCTGCTCACCGACGGTCGCTTCTCGGGCGGTACCTCGGGCCTGTCGATCGGCCACGCCTCGCCAGAGGCCGCGGCGGGCGGCGCCATCGGCCTGGTGCGCGCCGGCGACAAg                                                                                        >  4:156850/1‑136 (MQ=255)                                              cAAAGGCCTGGGCAAGGCCTGCGCCCTGCTCACCGACGGGCGCTTCTCGGGCGGTACCTCGGGCCTGTCGATCGGCCACGCCTCGCCAGAGGCCGCGGCGGGCGGCGCCATCGGCCTGGTGCGCGACGGCGACAAg                                                                                        <  3:177475/136‑1 (MQ=255)                                               aaaTGCCGGGCCAAGGCCTGCGCCCTGAGCACCTACGGGCGCTGCTCGGGCGGTACCTGGGGCCTGTCTATCGGCAACGCCTCGCAATAGGCCGCGGCTGGCGGCGCCATCGGCCTGGTGCGCGACGGCGACAAg                                                                                        <  7:215030/135‑1 (MQ=255)                                                      ggggCAAGGCCTGCGCCCTGCTCACCGACGGTCGCTTCGCGGGCGGTACCTCGGGCCTGTCGAGCGGCCACGCCTCGCCAGAGGCCGCGGCGGGCGGCGCCATCGGCCTGGTGCGCGACGGCGACAAGGTACTGAt                                                                                <  4:85934/135‑1 (MQ=255)                                                        ggaacggCGTGCGCCCTGCTCCCCGACGGGCGCTTGTCGGGCGTTACCTGGGGCCTGTGGATCGGCCACGCCTCGCCAGAGGCCGCGGCGGGCGGCGCCATCGGCCTGGTGCGCGACGGCGACAAGGTACTGATc                                                                               <  2:52885/130‑1 (MQ=255)                                                         gCAAGGCCTGCGCCCTGCTCACCGACGGTCGCTTCTCGGGCGGTACCTCGGGCCTGTCGATCGGCCACGCCTCGCCAGAGGCCGCGGCGGGCGGCGCCATCGGCCTGGTGCGCGCCGGCGACAAGGTCCTGATCg                                                                              >  5:82147/1‑135 (MQ=255)                                                         gCAAGGCCTGCGCCCTGCTCACCGACGGTCGCTTCTCGGGCGGTACCTCGGGCCTGTCGATCGGCCACGCCTCGCCAGAGGCCGCGGCGGGCGGCGCCATCGGCCTGGTGCGCGACGGCGACAAGGTACTGAt                                                                                <  6:174885/133‑1 (MQ=255)                                                         gCAAGGCCTGCGCCCTGCTCACCGACGGTCGCTTCTCGGGCGGTACCTCGGGCCTGTCGATCGGCCACGCCTCGCCAGAGGCCGCGGCGGGCGGCGCCATCGGCCTGGTGCGCGACGGCGACAAGGTACTGATCg                                                                              >  6:140119/1‑135 (MQ=255)                                                           aaGGCCTGCGCCCTGCTCACCGCCGGTCGCTTCTAGGGCGGGTCCTCGGGCCTGTCGCTCGGCCCCGCCTCGCCAGCCGCCGCGGCGGGCGGCGCCATCGGCCTGGTGCGCGTCGGCGCCAAGGTTCTGAGCgcc                                                                            >  3:213008/1‑133 (MQ=255)                                                           aaGGCCTGCGCCCTGCTCACCGACGGTCGCTTCTCTGGCGGTACCTCGGGCCTGTCGATCGGCCACGCCTCGCCAGAGGCCGCGGCGGGCGGCGCCATCGGCCTGGTGCGCGACGGCGACAAGGTACTGATCGCCa                                                                           >  3:168055/1‑136 (MQ=255)                                                                cTGCGCCCTGCTCACCGACGGTCGCTTCTCGGGCGGTACCTCGGGCCTGTCGATCGGCCACGCCTCGCCAGAGGCCGCGGCGGGCGGCGCCATCGGCCTGGTGCGCGACGGCGACAAGGTACTGATCGACATccc                                                                       >  3:139473/1‑135 (MQ=255)                                                                     cccTGCTCACCGACGGTCGCTTCTCGGGCGGTACCTCGGGCCTGTCGATCGGCCACGCCTCGCCAGCGGCCGCGGCGGGCGGCGCCATCGGCCTGGTGCGCGACGGCGACAAGGTACTGATCGACATCCCTAACCg                                                                 >  3:68787/1‑136 (MQ=255)                                                                     cccTGCTCACCGACGGTCGCTTCTCGGGCGGTACCTCGGGCCTGTCGATCGGCCACGCCTCGCCAGAGGCCGCGGCGGGCGGCGCCATCGGCCTGGTGCGCGACGGCGACAAGGTACTGATCGACATCCCTAAcc                                                                  >  2:253195/1‑135 (MQ=255)                                                                     cccTGCTCACCGACGGTCGCTTCTCGGGCGGGACCTCGGGCCTGTCGCTCGGCCACGCCTCGCCAGAGGCCGCGGCGGGCGGCCCCCAGGGCCGGGGGGGGGCCGGCGGCAAGGGTCTGATGGGCATCCCGAAcc                                                                  >  1:339129/1‑135 (MQ=255)                                                                           tCACCGACGGTCGCTTCTCGGGCGGTACCTCGGGCCTGTCGATCGGCCACGCCTCGCCAGAGGCCGCGGCGGGCGGCGCCATCGGCCTGGTGCGCGACGGCGACAAGGTACTGATCGACATCCCTAACCGCTCGAt                                                           >  4:175131/1‑136 (MQ=255)                                                                                gACGGTCGCTTCTCGGGCGGTACCTCGGGCCTGTCGATCGGCCACGCCTCGCCAGAGGCCGCGGCGGGCGGCGCCATCGGCCTGGTGCGCGACGGCGACAAGGTACTGATCGACATCCCTAACCGCTCGATCaa                                                        >  1:220741/1‑134 (MQ=255)                                                                                  cGGTCGCTTCTCGGGCGGTACCTCGGGCCTGTCGATCGGCCACGCCTCGCCAGAGGCCGCGGCGGGCGGCGCCATCGGCCTGGTGCGCGACGGCGCCAAGGTACTGATCGACCTCCCTAACCGCTCGCTCAACct                                                     >  8:195096/1‑135 (MQ=255)                                                                                  cGGTCGCTTCTCGGGCGGTACCTCGGGCCTGTCGATCGGCCACGCCTCGCCAGAGGCCGCGGCGGGCGGCGCCATCGGCCTGGTGCGCGACGGCGACAAGGTACTGATCGACATCCCTAACCGCTCGATCAACct                                                     >  2:68667/1‑135 (MQ=255)                                                                                          tctcGGGCGGTACCTCGGGCCTGTCGATCGGCCACGCCTCGCCAGAGGCCGCGGCGGGCGGCCCCATCGGCCTGGTGCGCGACGGCGACAAGGTACTGATCGACATCCCTAACCGCTCGATCAACCTGCTGGTCa                                             >  8:110587/1‑135 (MQ=255)                                                                                             ggggCGGTACCTCGGGCGTGTCGAGCGGCCACGCCGCGCCAGCGGCCGCGGCGGGCGGCGCCATCGGCCTGGTGCGCGACGGCGACAAGGTACTGATCGACATCCCTAACCGCTCGATCAACCTGCTGGTCAGCg                                          <  7:69741/134‑1 (MQ=255)                                                                                               ggCGGTACCTCGGGCCTGTCGATCGGCCACGCCTCGCCAGAGGCCGCGGCGGGCGGCGCCATCGGCCTGGTGCGCGACGGCGACAAGGTACTGATCGACATCCCTAACCGCTCGATCAACCTGCTGGTCAGCg                                          <  5:66822/133‑1 (MQ=255)                                                                                                   gaaCCGCGGGCCTGGCGATCGGCCACGCCTCGCCAGAGGCCGCGGCGGGCGGCGCCATCGGCCTGGTGCGCGACGGCGACAAGGTACTGATCGACATCCCTAACCGCTCGATCAACCTGCTGGTCAGCGATGAag                                    <  7:44549/133‑1 (MQ=255)                                                                                                   gTACCTCGGGCCTGGCGAGCGGCCACGCCGCGCCCGCGGCCGCGGCGGGCGGCGCCATCGGCCTGGTGCGCGACGGCGACAAGGTACTGATCGACATCCCTAACCGCTCGATCAACCTGCTGGGCAGCGATTAag                                    <  1:121194/135‑1 (MQ=255)                                                                                                   gTACCGGGGGCGTGGCGACCGCCCACGCCTCGCCAGCGGCCGCGGCGGGCGGCGCCATCGGCCTGGTGCGCGACGGCGACAAGGTACTGATCGACATCCCTAACCGCTCGATCAACCTGCTGGTCAGCGATGAag                                    <  1:113620/135‑1 (MQ=255)                                                                                                   gTACCGGGGGCCTGTCGATGGGCCCCGCCGCGCCAGAGGCCGCGGCGGGCGGCGCCATCGGCCTGGTGCGCGACGGCGACAAGGTACTGATCGACATCCCTAACCGCTCGATCAACCTGCTGGTCAGCGATGAag                                    <  2:332203/135‑1 (MQ=255)                                                                                                   gTACCGGGGGCCTGTCGAGCGGCCACGCCGCGCCAGCGGCCGCGGCGGGCGGCGCCATCGGCCTGGTGCGCGACGGCGACAAGGTACTGATCGACATCCCTAACCGCTCGATCAACCTGCTGGTCAGCGATGAag                                    <  1:118617/135‑1 (MQ=255)                                                                                                   gTACCGCGGGCCTGGCGAGCGGCCACGCCTCGCCCGCGGCCGCGGCGGGCGGCGCCATCGGCCTGGTGCGCGACGGCGACAAGGTACTGATCGACATCCCTAACCGCTCGATCAACCTGCTGGTCAGCGATGAag                                    <  5:183498/135‑1 (MQ=255)                                                                                                      ccTCGGGCCTGTCGATCGGCCACGCCTCGCCAGAGGCCGCGGCGGGCGGCGCCATCGGCCTGGTGCGCGACGGCGACCAGGTACTGATCGACATCCCTAACCGCTCGATGAACCAGCTGGTCAGCGATGAAgcgc                                 >  2:223473/1‑135 (MQ=255)                                                                                                       cTCGGGCCGGGCGCGCGGCCACGCCGCGCCCGCGGCCGCGGCGGGCGGCGCCATCGGCCTGGTGCGCGACGGCGACAAGGTACTGATCGACATCCCTAACCGCTCGATCAACCTGCTGGTCAGCGATGAAGAGCTg                               <  4:361059/136‑1 (MQ=255)                                                                                                        gcGGGCCTGCCGAGCGGCCACGCCGCGCCCGAGGCCGCGGCGGGCGGCGCCATCGGCCTGGTGCGCGACGGCGACAAGGTACTGATCGACATCCCTAACCGCTCGATCAACCTGCTGGTCAGCGATGAAGAGCTg                               <  8:320303/134‑1 (MQ=255)                                                                                                             ccTGTCGATCGGCCACGCCTCGCCAGAGGCCGCGGCGGGCGGCGCCATCGGCCTGGTGCGCGACGGCGACAAGGTACTGATCGACATCCCTAACCGCTCGATCAACCTGCTGGTCAGCGATGAAGAGCTGGCGCa                          >  6:165026/1‑135 (MQ=255)                                                                                                               ggTCGCTCGGCCCCGCCTCGCCGGCGGCCGCGGCGGGCGGCGCCATCGGCCTGGTGCGCGACGGCGACAAGGTACTGATCGACATCCCTAACCGCTCGATCAACCTGCTGGTCAGCGATGAAGAGCTGGCGCAAc                        <  2:157098/134‑1 (MQ=255)                                                                                                                  cGATCGGCCACGCCGCGCCAGAGGCCGCGGCGGGCGGCTCCATCGGCCTGGTGCGCGACGGCGACAAGGTTCTGATCGACATCCCTAACCGCTCGATCAACCTGCTGGTCAGCGATGAAGAGCTGGCGCAACGcc                     <  2:269829/135‑1 (MQ=255)                                                                                                                   gagcGGCCCCGCCGCGCCCGCGGCCGCGGCGGGCGGCGCCATCGGCCTGGTGCGCGACGGCGACAAGGTACTGATCGACATCCCTAACCGCTCGATCAACCTGCTGGTCAGCGATGAAGAGCTGGCGCAACGCCg                    <  1:303017/132‑1 (MQ=255)                                                                                                                   gATCGGCCACGCCTCGCCAGAGGCCGCGGCGGGCGGCGCCATCGGCCTGGTGCGCGCCGGCGACAAGGTACTGATCGACCTCCCTAACCGCTCGAGCAACCTGCTGGTCAGCGATGAAGAGCTGGCGCAACGCCgt                   >  3:265975/1‑136 (MQ=255)                                                                                                                   gATCGGCCACGCCTCGCCAGAGGCCGCGGCGGGCGGCGCCATCGGCCTGGTGCGCGACGGCGACAAGGTACTGATCGACATCCCTAACCGCTCGATCAACCTGCTGGTCAGCGATGAAGAGCTGGCGCCACGCCg                    >  7:320303/1‑135 (MQ=255)                                                                                                                   gATCGGCCACGCCTCGCCAGAGGCCGCGGCGGGCGGCGCCATCGGCCTGGTGCGCGACGGCGACAAGGTACTGATCGACATCCCTAACCGCTCGATCAACCTGCTGGTCAGCGATGAAGAGCTGGCGCAACGCCg                    >  7:208431/1‑135 (MQ=255)                                                                                                                    aTCGGCCACGCCTCGCCAGAGGCCGCGGCGGGCGGCGCCATCGGCCTGGTGCGCGACGGCGACAAGGTACTGATCGACATCCCTAACCGCTCGATCAACCTGCTGGTCAGCGATGAAGAGCTGGCGCGACGCCgt                   >  8:186566/1‑135 (MQ=255)                                                                                                                    aTCGGCCACGCCTCGCCAGAGGCCGCGGCGGGCGGCGCCATCGGCCTGGTGCGCGACGGCGACAAGGTACTGATCGACATCCCTAACCGCTCGATCAACCTGCTGGTCAGCGATGAAGAGCTGGCGCAACGCCgt                   >  6:97736/1‑135 (MQ=255)                                                                                                                       ggCCACGCCTCGCCCGCGGCCGCGGCGGGCGGCGCCATCGGCCTGGTGCGCGACGGCGACAAGGTACTGATCGACATCCCTAACCGCTCGATCAACCTGCTGGTCAGCGATGAAGAGCTGGCGCAACGCCGTGTc                <  5:169515/135‑1 (MQ=255)                                                                                                                       ggCCACGCCTCGCCAGAGGCCGCGGCGGGCGGCGCCATCGGCCTGGTGCGCGACGGCGACAAGGTACTGATCGACATCCCTAACCGCTCGATCAACCTGCTGGTCAGCGATGAAGAGCTGGCGCAACGCCGTGTc                <  1:253195/135‑1 (MQ=255)                                                                                                                       ggCCACGCCTCGCCAGAGGCCGCGGCGGGCGGCGCCATCGGCCTGGTGCGCGACGGCGACAAGGTACTGATCGACATCCCTAACCGCTCGATCAACCTGCTGGTCAGCGATGAAGAGCTGGCGCAACGCCGTGTc                <  6:365053/135‑1 (MQ=255)                                                                                                                       ggCCACGCCTCGCCAGAGGCCGCGGCGGGCGGCGCCATCGGCCTGGTGCGCGACGGCGACAAGGTACTGATCGACATCCCTAACCGCTCGATCAACCTGCTGGTCAGCGATGAAGAGCTGGCGCAACGCCGTGTc                <  5:97736/135‑1 (MQ=255)                                                                                                                       ggCCACGCCGCGCCCGCGGCCGCGGCGGGCGGCGCCATCGGCCTGGTGCGCGACGGCGACAAGGTACTGATCGACATCCCTAACCGCTCGATCAACCTGCTGGGCAGCGATGAAGAGCTGGCGCAACGCCGTGTc                <  3:159290/135‑1 (MQ=255)                                                                                                                          cccgccgcGCCCGCGGCCGCGGCGGGCGGCGCCAGCGGCCTGGTGCGCGACGGCGACAAGGTACTGATCGACATCCCTAACCGCTCGATCAACCTGCTGGTCAGCGATGAAGAGCTGGCGCAACGCCGTGTCGAg             <  8:321540/133‑1 (MQ=255)                                                                                                                             gCCGCGCCAGCGGCCGCGGCGGGCGGCGCCATCGGCCGGGTGCGCGACGGCGACAAGGTACTGATCGACATCCCTAACCGCTCGATCAACCTGCTGGTCAGCGATGAAGAGCTGGCGCAACGCCGTGTCGAGCAg          <  6:84225/135‑1 (MQ=255)                                                                                                                                  gCCAGAGGCCGCGGCGGGCGGCGCCATCGGCCTGGTGCGCGACGGCGACAAGGTACTGATCGACATCCCTAACCGCTCGATCAACCTGCTGGTCAGCGATGAAGAGCTGGCGCAACGCCGTGTCGAGCAGGACa      <  3:318285/134‑1 (MQ=255)                                                                                                                                     agagGCCGCGGCGGGCGGCGCCATCGGCCTGGTGCGCGACGGCGACAAGGTACTGATCGACATCCCTAACCGCTCGATCAACCTGCTGGTCAGCGATGAAGAGCTGGCGCAACGCCGTGTCGAGCAGGACaagaa  >  2:104925/1‑135 (MQ=255)                                                                                                                                       |                                                                                                                                     CCCGGGCATGCAAGAAATGCTCTACCCGACCTCGTACCTCAAGTCCAAAGGCCTGGGCAAGGCCTGCGCCCTGCTCACCGACGGTCGCTTCTCGGGCGGTACCTCGGGCCTGTCGATCGGCCACGCCTCGCCAGAGGCCGCGGCGGGCGGCGCCATCGGCCTGGTGCGCGACGGCGACAAGGTACTGATCGACATCCCTAACCGCTCGATCAACCTGCTGGTCAGCGATGAAGAGCTGGCGCAACGCCGTGTCGAGCAGGACAAGAA  >  KT2440/5844582‑5844848

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑