Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	KT2440	5,947,570	T→G	15.6%	L169R (CTG→CGG)  Q423P (CAG→CCG)	CJ019_05568 → CJ019_05569 ←	hypothetical protein hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	5,947,570	0	T	G	15.6%	131.3 / 3.1	58	L169R (CTG→CGG)  Q423P (CAG→CCG)	CJ019_05568 CJ019_05569	hypothetical protein hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (30/17);  new base G (2/9);  total (32/26)
Fisher's exact test for biased strand distribution p-value = 8.15e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTTGAACGGTATTGGCGCGGGCGTCTGGGCAGTGGCCCTGGGCGCCGCCGCCTACCACTTCGGCGCCATCCTCGAAGGCTTGCTGGGCAACGTCAAGCGTTACGAGCTATGGGTGCTTGGCGGCCTGGTGGCGCTGGGCGGCCTGCTGTGGCTGCGCCGGCGCTTCCGTACTCTGCGCGCGGAGCGCAGGGCGGCGAGCCAGGCCCAGCCCCCAGAGGCTGAGCAGGCTGTAAGCCACGAGCAGAAGCCAGGTCAGCGGCACGACCACC  >  KT2440/5947436‑5947704                                                                                                                                       |                                                                                                                                       gTTGAACGGTATTGGCGCTGGCGTCTGTGCAGTGGCCCTGGGCGCCGCCGCCTACCACTTAGGCGCCATCCTCGCAGGCTTGCTGGGCAACGGCCAGCGTTACGAGCTATGTGTGCGTGGCGGCCTGGTGGCGCt                                                                                                                                        >  6:106619/1‑135 (MQ=255)   tGAACGGTATTGGCGCGGGCGTCTGGGCAGTGGCCCTGGGCGCCGCCGCCTACCACTTCGGCGCCATCCTCGAAGGCTTGCTGGGCAACGTCAAGCGTTACGAGCTATGGGTGCTTGTCGGCCTGGTGGCGCTgg                                                                                                                                      >  8:172836/1‑135 (MQ=255)      aCGGTATTGGCGCGGGCGTCTGGGCAGTGGCCCTGGGCGCCGCCGCCTACCACTTCGGCGCCATCCTCGAAGGCTTGCTGGGCAACGTCAAGCGTTACGAGCTATGGGTGCTTGGCGGCCTGGTGGCGCTGggc                                                                                                                                    <  7:307428/134‑1 (MQ=255)      aCGGTATGGGCGCGTGCGTCGTGGCAGTGGCACTGGGCGCCGCCGCCTACCACTTCGGCGCCATCCTCGCAGGCTTGCTGGGCAACGTCAAGCGTTACGAGCTATGGGGGCTTGGCGGCCTGGTGGCGCTGggc                                                                                                                                    <  7:70233/134‑1 (MQ=255)               gcgcGGGCGTCTGGGCAGTGGCCCTGGGCGCCGCCGCCTACCACTTCGGCGCCATCCTCGCAGGCTTGCTGGGCAACGTCAAGCGTTACGAGCTATGGGTGCTTGGCGGCCTGGTGGCGCTGGGCGGCCTGCtgtg                                                                                                                         >  4:105172/1‑136 (MQ=255)                      cGTCTGGGCAGTGGCCCTGGGCGCCGCCGCCTACCACTTCGGCGCCATCCTCGAAGGCTTGCTGGGCAACGTCAAGCGTTACGAGCTATGGGTGCTTGGCGGCCTGGTGGCGCTGGGCGGCCTGCTGTGGCTgcgc                                                                                                                  <  3:233718/136‑1 (MQ=255)                       gTCTGGGCAGTGGCCCTGGGCGCCGCCGCCTACCCCTTCGGCGCCCTCCTCGAAGGCTTGCTGGGCAACGGCAAGCGGTTCGCGCGATGGGTGCTTGGCGGCCTGGTGGCGCTGGGCGGCCTGCTGTGGCTgcgc                                                                                                                  >  5:28742/1‑135 (MQ=255)                       gTCTGGGCAGTGGCCCTGGGCGCCGCCGCCTACCCCTTCGGCGCCATCCTCGCAGGCGTGCTGGGCAACGTCAAGCGTTACGAGCTATGGGTGCGTGGCGGCCTGGTGGCGCTGGGCGGCCTGCTGTGGCTgcgc                                                                                                                  >  7:340087/1‑135 (MQ=255)                       gTCTGGGCAGTGGCCCTGGGCGCCGCCGCCTACCCCTTCGGCGCCATCCGCGCAGGCGTGGTGGGCCACGTCAAGCGTTCCGAGCTATGGGTGGTTGGCGGCCTGGTGGCGCTGGGCGGCCTGCTGTGGCTgcgc                                                                                                                  >  5:256402/1‑135 (MQ=255)                       gTCTGGGCAGTGGCCCTGGGCGCCGCCGCCTACCACTTCGGCGCCATCCTCGAAGGCTTGCTGGGCAACGTCAAGCGTTACGAGCTATGGGTGCTTGGCGGCCTGGTGGCGCTGGGCGGCCTGCTGTGGCTgcgc                                                                                                                  >  6:187593/1‑135 (MQ=255)                       gTCTGGGCAGTGGCCCTGGGCGCCGCCGCCTACCACTTCGGCGCCATCCTCGAAGGCTTGCTGGGCAACGTCAAGCGTTACGAGCTATGGGTGCTTGGCGGCCTGGTGGCGCTGGGCGGCCTGCTGTGGCTgcgc                                                                                                                  <  7:29482/135‑1 (MQ=255)                       gTCTGGGCAGTGGCCCTGGGCGCCGCCGCCTACCACTTCGGCGCCATCCTCGAAGGCTTGCTGGGCAACGTCAAGCGTTACGAGCTATGGGTGCTTGGCGGCCTGGTGGCGCTGGGCGGCCTGCTGTGGCTGCGcc                                                                                                                 >  4:84502/1‑136 (MQ=255)                       gTCTGGGCAGTGGCCCTGGGCGCCGCCGCCTACCACTTCGGCGCCATCCTCGAAGGCGTGCTGGGCACCGTCAAGCGTTACGAGCTATGGGTGCTTGGCGGCCTGGTGGCGCTGGGCGGCCTGCTGTGGCTGCGcc                                                                                                                 >  3:305102/1‑136 (MQ=255)                       gTCTGGGCAGTGGCCCTGGGCGCCGCCGCCTACCACTTCGGCGCCATCCTCGAACGCTTGCGGGGCAACCTCAAGCGTTACGAGCGATGGGTGGTTGGCGGCCGGGTGGCGCTGGGCGGCCGGCGGTGGCTgcgc                                                                                                                  >  5:265482/1‑135 (MQ=255)                       gTCTGGGCAGTGGCCCTGGGCGCCGCCGCCTACCACTTCGGCGCCATACTCGCAGGCGTGCTGGGCAACCTCTAGCGTGACGCGCTATGGGTGCTTGGCGGCCTGGTGGCGCTGGGCGGCCTGCTGTGGCTgcgc                                                                                                                  >  7:354439/1‑135 (MQ=255)                       gTCTGGGCAGTGGCCCTGGGCGCCGCCGCCGACCCCTTGGGCGCCCTCCGCGAAGGCTTGCTGGGCAACGGCACGGGGTACGCGGTATGGGTGCTTTGCGGCCTGGGGGCGCGGGGCGGCCGGCTGTGGCTgcgc                                                                                                                  >  1:144442/1‑135 (MQ=255)                       gTCTGGGCAGTGGCCCTGGGCGCCGCCGCCGACCACTTCGGCGCCCTCCTCCAAGGCGTGCTGGGCAACGTCAAGCGGTACGAGCTATGGGTGCTTGGCGGCCGGGTGGCGGGGGGCGGCCTGCTGTGGCTgcgc                                                                                                                  >  5:9329/1‑135 (MQ=255)                        tCTGGGCAGTGGCCCTGGGCGCCGCCGCCTACCACTTCGGCGCCATCCTCGAAGGCTTGCTGGGCAACGTCAAGCGTTACGAGCTATGGGTGCTTGGCGGCCTGGTGGCGCTGGGCGGCCTGCTGTGGCTGCGCCg                                                                                                                <  3:15302/136‑1 (MQ=255)                        tCTGGGCAGGGGCCCTGGGCGCCGCCGCCTACCACTTCGGCGCCATCCTCGAAGGCTTGCTGGGCAACGTCAAGCGTTACGAGCTATGGGTGCTTGGCGGCCTGGTGGCGCTGGGCGGCCTGCTGTGGCTGCGCCg                                                                                                                <  4:119862/136‑1 (MQ=255)                         cTGGGCAGTGGCCCTGGGCGCCGCCGCCTACCACTTCGGCGCCATCCTCGAAGGCTTGCTGGGCAACGTCAAGCGTTACGAGCTATGGGTGCTTGGCGGCCTGGTGGCGCTGGGCGGCCTGCTGTGGCTGCGCCg                                                                                                                <  6:261382/135‑1 (MQ=255)                         cTGGGCAGTGGCCCTGGGCGCCGCCGCCTACCACTTCGGCGCCATCCTCGAAGGCTTGCTGGGCAACGTCAAGCGTTACGAGCTATGGGTGCTTGGCGGCCTGGGGGCGCTGGGCGGCCTGCTGTGGCTGCGCCg                                                                                                                <  6:331902/135‑1 (MQ=255)                          tGGGCAGTGGCCCTGGGCGCCGCCGCCTACCACTTCGGCGCCATCCTCGAAGGCTTGCTGGGCAACGTCAAGCGTTACGAGCTATGGGTGCTTGGCGGCCTGGTGGCGCTGGGCGGCCTGCTGTGGCTGCGCCgg                                                                                                               <  5:187593/135‑1 (MQ=255)                                  ggCCCTGGGCGCCGCCGCCTACCACTTCGGCGCCATCCTCGAAGGCTTGCTGGGCAACGTCAAGCGTTACGAGCTATGGGTGCTTGGCGGCCTGGTGGCGCTGGGCGGCCTGCTGTGGCTGCGCCGGCGCTTcc                                                                                                        >  2:113043/1‑134 (MQ=255)                                    cccTGGGCGCCGCCGCCTACCACTTCGGCGCCATCCTCGAAGGCTTGCTGGGCAACGTCAAGCGTTACGAGCTATGGGTGCTTGGCGGCCTGGTGGCGCTGGGCGGCCTGCTGTGGCTGCGCCGGCGCTTCCGTa                                                                                                     >  7:354628/1‑135 (MQ=255)                                    cccTGGGCGCCGCCGCCTACCACTTCGGCGCCATCCTCGAAGGCTTGCTGGGCAACGTCAAGCGTTACGAGCTATGGGTGCTTGGCGGCCTGGTGGCGCTGGGCGGCCTGCTGTGGCTGCGCCGGCGCTTCCGTa                                                                                                     >  2:266364/1‑135 (MQ=255)                                    cccTGGGCGCCGCCGCCTACCACTTCGGCGCCATCCTCGAAGGCTTGCTGGGCAACGTCAAGCGTTACGAGCTATGGGTGCTTGGCGGCCTGGTGGCGCTGGGCGGCCTGCTGTGGCTGCGCCGGCGCTTCCGTa                                                                                                     >  5:307634/1‑135 (MQ=255)                                          gcgcCGCCGCCTACCACTTCGGCGCCATCCTCGAAGGGTTGCTGGGAAACGTCAAGCGTTACGAGCTATGGGTGCTTGGCGGCCGGGTGGCGCTGGGCGGCCTGCTGTGGCTGCGCGGGCGCTTCCGTACTCTgcg                                                                                              <  4:249759/136‑1 (MQ=255)                                          gcgcCGCCGCCTACCACTTCGGCGCCAGCCTCGAAGGCTTGCTGGGCAACGTCAAGCGTTACGAGCTATGGGTGCTTGGCGGCCTGGTGGCGCTGGGCGGCCTGCTGTGGCTGCGCCGGCGCTTCCGTACTCTgc                                                                                               <  8:325666/135‑1 (MQ=255)                                            gccgccgccTACCACTTCGGCGCCATCCTCGAAGGCTTGGTGGGCAACGTCAAGCGTTACGAGCTATGGGGGCTTGGCGGCGTGGGGGCGCTGGGCGGCCTGCGGTGGCTGCGCCGGCGCTTCCGTACTCTgcgc                                                                                             <  7:172836/135‑1 (MQ=255)                                            gccgccgccTACCACTTCGGCGCCATCCTCGAAGGCTTGCTGGGCAACGTCAAGCGTTACGAGCGAGGGGTGCTTGGCGGCCTGGTGGCGCTGGGCGGCCTGCTGTGGCTGCGCCGGCGCTTCCGTACTCTgcgc                                                                                             >  7:111971/1‑135 (MQ=255)                                                                   cATCCTCGAAGGCTTGCTGGGCAACGTCAAGCGTTACGAGCTATGGGTGCTTGGCGGCCTGGTGGCGCTGGGCGGCCTGCTGTGGCTGCGCCGGCGCTTCCGTACTCTGCGCGCGGAGCGCAGGGCGGCGAGCCa                                                                      >  2:6625/1‑135 (MQ=255)                                                                   cATCCTCGAAGGCTTGCTGGGCAACGTCAAGCGTTACGAGCTATGGGTGCTTGGCGGCCTGGTGGCGCTGGGCGGCCTGCTGTGGCTGCGCCGGCGCTTCCGTACTCTGCGCGCGGAGCGCAGGGCGGCGAGCCa                                                                      >  2:234505/1‑135 (MQ=255)                                                                   cATCCTCGAAGGCTTGCTGGGCAACGTCAAGCGTTACGAGCTATGGGTGCTTGGCGGCCTGGTGGCGCTGGGCGGCCTGCTGTGGCTGCGCCGGCGCTTCCGTACTCTGCGCGCGGAGCGCAGGGCGGCGAGCCAg                                                                     >  4:222328/1‑136 (MQ=255)                                                                    aTCCGCGAAGGCTGGCTGGGCAACGTCAAGCGTTACGAGCTATGGGGGCTGGGCGGCCGGGTGGCGCGGGGCGGCCTGCTGTGGCGGCGCCGGCGCTTCCGTACTCTGCGCGCGGAGCGCAGGGCGGCGAGCCAg                                                                     <  6:1071/135‑1 (MQ=255)                                                                       cTCGACGGCTTCCGGGGCACCGCCACGCGTACCGCGCTATGGGTGCTGGGCGGCCGGGGGGCGCGGGGCGGCCTGCGGGGGCTGCGCCGGCGCTCCCGTCCGCTGCGCGCGGCGCGCAGGGCGGCGAGCCAGGcc                                                                  <  5:184179/135‑1 (MQ=255)                                                                        tCGACGGCTTGCGGGGCAACGGCAAGCGTTACGAGCTATGGGGGCTTGGCGGCCGGGTGGCGCTGGGCGGCCGGCTGGGGCGGCGCCGGCGCTTCGGTACTCCGCGCGCGCCGCGCAGGGCGGCGAGCCAGGcc                                                                  <  2:89952/134‑1 (MQ=255)                                                                              gCTGGCGGGGCAACGCCATGGGTTCCGCGCTAGGGGGGCTGGGCGGCGGGGGGGCGCGGGGCGGCGGGCGGGGGCTGCGCCGGCGCTCCCGTCCTCGGCGCGCGGCGCGCAGGGCGGCGAGCCAGGCCCAGcccc                                                           <  6:337550/135‑1 (MQ=255)                                                                                  gCTGGGCAACGTCAAGCGTTACGAGCTATGGGTGCTTGGCGGCCTGGTGGCGCTGGGCGGCCTGCTGTGGCTGCGCCg                                                                                                                <  2:110817/78‑1 (MQ=255)                                                                                  gCTGGGCAACGTCAAGCGTTACGAGCTATGGGTGCTTGGCGGCCTGGTGGCGCTGGGCGGCCTGCTGTGGCTGCGCCg                                                                                                                >  1:110817/1‑78 (MQ=255)                                                                                  gCTGGGCAACGTCAAGCGTTACGAGCTATGGGTGCTTGGCGGCCTGGTGGCGCTGGGCGGCCTGCTGTGGCTGCGCCGGCGCTTCCGTACTCTGCGCGCGGAGCGCAGGGCGGCGAGCCAGGCCCAGCCCCCaga                                                       >  7:22625/1‑135 (MQ=255)                                                                                    tGGGCAACGTCAAGCGTTACGAGCTATGGGTGCTTGGCGGCCTGGTGGCGCTGGGCGGCCTGCTGTGGCTGCGCCGGCGCTTCCGTACTCTgc                                                                                               <  4:81679/93‑1 (MQ=255)                                                                                    tGGGCAACGTCAAGCGTTACGAGCTATGGGTGCTTGGCGGCCTGGTGGCGCTGGGCGGCCTGCTGTGGCTGCGCCGGCGCTTCCGTACTCTgc                                                                                               >  3:81679/1‑93 (MQ=255)                                                                                     gggCAACGCCACGCGTTACGAGCTAGGGGTGCTGGGCGGCCTGGGGGCGCGGGGCGGCCGGCTGGGGCGGCGCGGGCGCTGCCGTACTCTGCGCGCGGCGCGCAGGGCGGCGAGCCAGGCCCAGCCCCCAGAGGCt                                                   <  4:57035/136‑1 (MQ=255)                                                                                       gaaaCGTCAAGCGTTACGAGCTAGGGGGGCTGGGCGGCCTGGGGGCGGGGGGCGGCCGGCTGTGGCTGCGCCGGCGCTGCCGTACTCTGCGCGCGGAGCGCAGGGCGGCGAGCCAGGCCCAGCCCCCAGAGGCTg                                                  <  8:354628/133‑1 (MQ=255)                                                                                       gCAACGTCACGCGTTACGAGCTATGGGGGCTTGGCGGCCTGGGGGCGCGGGGCGGCCTGCTGTGGCTGCGCCGGCGCTTCCGAACTCTGCGCGCGGAGCGCAGGGCGGCGAGCCAGGCCCAGCCCCCAGAGGCTg                                                  <  2:239670/135‑1 (MQ=255)                                                                                              cAAGCGTTACGAGCTATGGGTGCTTGACGGCCTGGTGGCGCTGGGCGGCCTGCTGTGGCTGCGCCGGCGCTTCAGTACTCTGCGCGCGGAGCGCACGGCGGCGAGCCAGGCCCAGCCCCCAGACGCTCAGCAGGCt                                          >  3:26451/1‑136 (MQ=255)                                                                                                          gCTTTGGGTGCGTGGCGGCCGGGGGGCGCTGGGCGGCCGGCGGGGGCGGCGCCGGCGCTTCCGTACTCTGCGCGCGGAGCGCAGGGCGGCGAGCCAGGCCCAGCCCCCAGAGGCTGAGCAGGCTGTAAGCCACGa                               <  2:38601/135‑1 (MQ=255)                                                                                                               gggTGCTTGGCGGCCTGGTGGCGCTGGGCGGCCTGCTGTGGCTGCGCCGGCGCTTCCGTACTCTGCGCGCGGAGCGCAGGGCGGCGAGCCAGGCCCAGCCCCCAGAGGCTGAGCAGGCTGTAAGCCACGAGCa                            >  6:266481/1‑133 (MQ=255)                                                                                                               gggTGCTTGGCGGCCTGGTGGCGCTGGGCGGCCTGCTGTGGCTGCGCCGGCGCTTCCGTACTCTGCGCGCGGAGCGCAGGGCGGCGAGCCAGGCCCAGCCCCCAGAGGCTGAGCAGGCTGTAAGACACGAGCa                            >  5:343550/1‑133 (MQ=255)                                                                                                                   gCTTGGCGGCCTGGTGGCGCTGGGCGGCCTGCTGTGGCTGCGCCGGCGCTTCCGTACTCTGCGCGCGGAGCGCAGGGCGGCGCGCCAGGCCCAGCCCCCAGAGGCTGAGCAGGCTGTAAGCCACGAGCAGAAGc                       >  4:300229/1‑134 (MQ=255)                                                                                                                       ggcggcCTGGTGGCGCTGGGCGGCCTGCTGTGGCTGCGCCGGCGCTTCCGTACTCTGCGCGCGGAGCGCAGGGCGGCGAGCCAGGCCCAGCCCCCAGAGGCTGAGCAGGCTGTAAGCCACGAGCAGAAGCCAgg                   >  7:104475/1‑134 (MQ=255)                                                                                                                       ggcggcCTGGTGGCGCTGGGCGGCCTGCTGTGGCTGCGCCGGCGCTTCCGTACTCTGCGCGCGGAGCGCAGGGCGGCGAGCCAGGCCCAGCCCCCAGAGGCTGAGCAGGCTGTAAGCCACGAGCAGAAGCCAgg                   >  7:311650/1‑134 (MQ=255)                                                                                                                       ggcggcCTGGTGGCGCTGGGCGGCCTGCTGTGGCTGCGCCGGCGCTTCCGTACTCTGCGCGCGGAGCGCAGGGCGGCGAGCCAGGCCCAGCCCCCAGAGGCTGAGCAGGCTGTAAGCCACGAGCAGAAGCCAgg                   >  2:313999/1‑134 (MQ=255)                                                                                                                       ggcggcCTGGTGGCGCTGGGCGGCCTGCTGTGGCTGCGCCGGCGCTTCCGTACTCTGCGCGCGGAGCGCAGGGCGGCGAGCCAGGCCCAGCCCCCAGAGGCTGAGCAGGCTGTAAGCCACGAGCAGAAGCCAgg                   >  1:60317/1‑134 (MQ=255)                                                                                                                        gcggcCGGGGGGGGGGGGGGGGCGTGGTGTGGCGGCGCGGGCGCTCCGGTCCTCTGCGCGCGCCGGGCAGGGCGGCGAGCCAGGCCCAGCCCCCAGAGGCTGAGCAGGCTGTAAGCCACGAGCAGAAGCCAGGTc                 <  1:113043/135‑1 (MQ=255)                                                                                                                           gcCGGGGGGCGGGGGGCGGCCGGCGGGGGCGGCGCCGGCGCTGCCGTACGCGCCGCGCGGAGCGCAGGGCGGCGAGCCAGGCCCAGCCCCCAGAGGCTGAGCAGGCTGTAAGCCACGAGCAGAAGCCAGGTCAGc              <  6:307634/135‑1 (MQ=255)                                                                                                                               ggtggCGCTGGGCGGCCTGCTGTGGCTGCGCCGGCGCTTCCGTACTCTGCGCGCGGAGCGCAGGGCGGCGAGCCAGGCCCAGCCCCCAGAGGCTGAGCAGGCTGTAAGCCACGAGCAGAAGCCAGGTCAGCGGCAc         >  4:231969/1‑136 (MQ=255)                                                                                                                                ggggCGCGGGGCGGCCGGCGGTGGCGGCGCCGGCGCTCCCGTACCCGGCGCGCGGAGCGCAGGGCGGCGAGCCAGGCCCAGCCCCCAGAGGCTGAGCAGGCTGTAAGCCACGAGCAGAAGCCAGGTCAGCGGCAc         <  8:340087/133‑1 (MQ=255)                                                                                                                                      cTGGGCGGCCTGCTGTGGCTGCGCCGGCGCTGCCGTACTCTGCGCGCGGCGCGCAGGGCGGCGAGCCAGGCCCAGCCCCCAGAGGCTGAGCAGGCTGTAAGCCACGAGCAGAAGCCAGGTCAGCGGCACGaccacc  <  3:105172/136‑1 (MQ=255)                                                                                                                                       |                                                                                                                                       GTTGAACGGTATTGGCGCGGGCGTCTGGGCAGTGGCCCTGGGCGCCGCCGCCTACCACTTCGGCGCCATCCTCGAAGGCTTGCTGGGCAACGTCAAGCGTTACGAGCTATGGGTGCTTGGCGGCCTGGTGGCGCTGGGCGGCCTGCTGTGGCTGCGCCGGCGCTTCCGTACTCTGCGCGCGGAGCGCAGGGCGGCGAGCCAGGCCCAGCCCCCAGAGGCTGAGCAGGCTGTAAGCCACGAGCAGAAGCCAGGTCAGCGGCACGACCACC  >  KT2440/5947436‑5947704

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑